MS019014 (gene) Bitter gourd (TR) v1

Overview


Name	MS019014
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold20: 706190 .. 706459 (-)
RNA-Seq Expression	MS019014
Synteny	MS019014

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGCATGGAGGCCTCCTCCCCTGGGGTGTGTGGTGAAGATGAACACGGATGCGGCTGTGTCGACAGAGGGATCGGGTGTTGGTGTTCTGCTTCGTGACGGAAATGCGGAGATTGTGGCGGCCATGGTTGATTTTCATGTGGTGAAGACTCCTCTACTGACTGAAATTTTGGCAATCAGGGAAGGTTTGAGGCTGGCAACAAGGTTGGGGGTCCATCGAGTCATGGTGGAGTCGGATTCACTTGAAGCCCTAAATTTAATTGGGGACAAA

mRNA sequence

AATGCATGGAGGCCTCCTCCCCTGGGGTGTGTGGTGAAGATGAACACGGATGCGGCTGTGTCGACAGAGGGATCGGGTGTTGGTGTTCTGCTTCGTGACGGAAATGCGGAGATTGTGGCGGCCATGGTTGATTTTCATGTGGTGAAGACTCCTCTACTGACTGAAATTTTGGCAATCAGGGAAGGTTTGAGGCTGGCAACAAGGTTGGGGGTCCATCGAGTCATGGTGGAGTCGGATTCACTTGAAGCCCTAAATTTAATTGGGGACAAA

Coding sequence (CDS)

AATGCATGGAGGCCTCCTCCCCTGGGGTGTGTGGTGAAGATGAACACGGATGCGGCTGTGTCGACAGAGGGATCGGGTGTTGGTGTTCTGCTTCGTGACGGAAATGCGGAGATTGTGGCGGCCATGGTTGATTTTCATGTGGTGAAGACTCCTCTACTGACTGAAATTTTGGCAATCAGGGAAGGTTTGAGGCTGGCAACAAGGTTGGGGGTCCATCGAGTCATGGTGGAGTCGGATTCACTTGAAGCCCTAAATTTAATTGGGGACAAA

Protein sequence

NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIREGLRLATRLGVHRVMVESDSLEALNLIGDK

Homology

[Close]

BLAST of MS019014 vs. NCBI nr
Match: XP_022155289.1 (uncharacterized protein LOC111022426 [Momordica charantia])

HSP 1 Score: 177.6 bits (449), Expect = 4.9e-41
Identity = 89/90 (98.89%), Postives = 90/90 (100.00%), Query Frame = 0


Query: 1   NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIR 60
           NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAM+DFHVVKTPLLTEILAIR
Sbjct: 129 NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMIDFHVVKTPLLTEILAIR 188

Query: 61  EGLRLATRLGVHRVMVESDSLEALNLIGDK 91
           EGLRLATRLGVHRVMVESDSLEALNLIGDK
Sbjct: 189 EGLRLATRLGVHRVMVESDSLEALNLIGDK 218

[Close]

BLAST of MS019014 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 149.1 bits (375), Expect = 1.9e-32
Identity = 78/90 (86.67%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 1   NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIR 60
           N WR PPLG VVKMNTDAAVS EGSGVGVLLR+GNAEIV AMVDFH+VKTPLL +ILAIR
Sbjct: 65  NEWR-PPLGGVVKMNTDAAVSEEGSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIR 124

Query: 61  EGLRLATRLGVHRVMVESDSLEALNLIGDK 91
           EGL LATRLGVHRV+VE+DSLEALNLIGDK
Sbjct: 125 EGLSLATRLGVHRVVVETDSLEALNLIGDK 153

[Close]

BLAST of MS019014 vs. NCBI nr
Match: XP_042939527.1 (uncharacterized protein LOC122274566 [Carya illinoinensis])

HSP 1 Score: 64.7 bits (156), Expect = 4.7e-07
Identity = 39/88 (44.32%), Postives = 56/88 (63.64%), Query Frame = 0


Query: 3   WRPPPLGCVVKMNTDAAVSTEGS--GVGVLLRDGNAEIVAAMVDFH-VVKTPLLTEILAI 62
           WRPPP G V+K+NTD A+  E    GVGV+LRD N ++V A  +       P+  E+LAI
Sbjct: 76  WRPPPTG-VLKLNTDGAMFQEQCKIGVGVILRDENGKVVMATCNKEPEFSEPMEVELLAI 135

Query: 63  REGLRLATRLGVHRVMVESDSLEALNLI 88
             GL+L   +G++ ++VE DSL ++N I
Sbjct: 136 LRGLQLCVPMGINDLIVEIDSLLSVNAI 162

[Close]

BLAST of MS019014 vs. NCBI nr
Match: KAG6693248.1 (hypothetical protein I3842_10G159900, partial [Carya illinoinensis])

HSP 1 Score: 64.3 bits (155), Expect = 6.1e-07
Identity = 35/90 (38.89%), Postives = 57/90 (63.33%), Query Frame = 0


Query: 3   WRPPPLGCVVKMNTDAAVSTE--GSGVGVLLRDGNAE-IVAAMVDFHVVKTPLLTEILAI 62
           W PPP+GC +K+N D A  ++   +GVGV+LRD N + +VAA      V + +  E +A+
Sbjct: 581 WHPPPIGC-LKLNVDGATFSDSFSAGVGVILRDHNGDVVVAASKVEREVSSAVFIEAIAL 640

Query: 63  REGLRLATRLGVHRVMVESDSLEALNLIGD 90
             GL+L  + GV ++++ESDSL  +N + +
Sbjct: 641 LRGLQLCVQWGVPKIILESDSLILVNCLNE 669

[Close]

BLAST of MS019014 vs. NCBI nr
Match: KAG6693249.1 (hypothetical protein I3842_10G159900 [Carya illinoinensis])

HSP 1 Score: 64.3 bits (155), Expect = 6.1e-07
Identity = 35/90 (38.89%), Postives = 57/90 (63.33%), Query Frame = 0


Query: 3   WRPPPLGCVVKMNTDAAVSTE--GSGVGVLLRDGNAE-IVAAMVDFHVVKTPLLTEILAI 62
           W PPP+GC +K+N D A  ++   +GVGV+LRD N + +VAA      V + +  E +A+
Sbjct: 577 WHPPPIGC-LKLNVDGATFSDSFSAGVGVILRDHNGDVVVAASKVEREVSSAVFIEAIAL 636

Query: 63  REGLRLATRLGVHRVMVESDSLEALNLIGD 90
             GL+L  + GV ++++ESDSL  +N + +
Sbjct: 637 LRGLQLCVQWGVPKIILESDSLILVNCLNE 665

[Close]

BLAST of MS019014 vs. ExPASy TrEMBL
Match: A0A6J1DPU1 (uncharacterized protein LOC111022426 OS=Momordica charantia OX=3673 GN=LOC111022426 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.4e-41
Identity = 89/90 (98.89%), Postives = 90/90 (100.00%), Query Frame = 0


Query: 1   NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIR 60
           NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAM+DFHVVKTPLLTEILAIR
Sbjct: 129 NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMIDFHVVKTPLLTEILAIR 188

Query: 61  EGLRLATRLGVHRVMVESDSLEALNLIGDK 91
           EGLRLATRLGVHRVMVESDSLEALNLIGDK
Sbjct: 189 EGLRLATRLGVHRVMVESDSLEALNLIGDK 218

[Close]

BLAST of MS019014 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 9.1e-33
Identity = 78/90 (86.67%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 1   NAWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIR 60
           N WR PPLG VVKMNTDAAVS EGSGVGVLLR+GNAEIV AMVDFH+VKTPLL +ILAIR
Sbjct: 65  NEWR-PPLGGVVKMNTDAAVSEEGSGVGVLLREGNAEIVGAMVDFHMVKTPLLAKILAIR 124

Query: 61  EGLRLATRLGVHRVMVESDSLEALNLIGDK 91
           EGL LATRLGVHRV+VE+DSLEALNLIGDK
Sbjct: 125 EGLSLATRLGVHRVVVETDSLEALNLIGDK 153

[Close]

BLAST of MS019014 vs. ExPASy TrEMBL
Match: M8ARB3 (Casein kinase I-2-like protein OS=Aegilops tauschii OX=37682 GN=F775_24143 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.0e-08
Identity = 41/88 (46.59%), Postives = 54/88 (61.36%), Query Frame = 0


Query: 1   NAWRPPPLGCVVKMNTDAAV--STEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILA 60
           + WRPP LG  VK+NTDAAV  S E SG G + R  NA + A     H V  P++ E LA
Sbjct: 790 HGWRPPELG-EVKINTDAAVRHSAEMSGAGGVARSKNALLGAWCKPLHGVTDPMIIEALA 849

Query: 61  IREGLRLATRLGVHRVMVESDSLEALNL 87
           +R+G+  A   G  +VM+E+D LE +NL
Sbjct: 850 LRDGVIFANLRGYAQVMMETDCLEIVNL 876

[Close]

BLAST of MS019014 vs. ExPASy TrEMBL
Match: A0A7J6WZQ1 (RNase H domain-containing protein OS=Thalictrum thalictroides OX=46969 GN=FRX31_007511 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.9e-07
Identity = 28/86 (32.56%), Postives = 52/86 (60.47%), Query Frame = 0


Query: 2  AWRPPPLGCVVKMNTDAAVSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIRE 61
          +W PPP G  + +NTD A+  +G+G   +LR+   +++ A    H   + L  E++A+  
Sbjct: 12 SWEPPPQGW-ISLNTDGALGQQGAGYAGILRNEKGQVLNAYTAGHKSNSVLFLEMIAVER 71

Query: 62 GLRLATRLGVHRVMVESDSLEALNLI 88
          GL LA  +G+ R+MV++DS++   ++
Sbjct: 72 GLELAEHMGLRRIMVQTDSMQVARIL 96

[Close]

BLAST of MS019014 vs. ExPASy TrEMBL
Match: A0A6J1CK80 (uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012047 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.9e-07
Identity = 36/68 (52.94%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 3   WRPPPLGCVVKMNTDAA--VSTEGSGVGVLLRDGNAEIVAAMVDFHVVKTPLLTEILAIR 62
           W+PP  G  VK+N DAA  V  +  GVGVL+RD    I+AA++  H V +PLL EI AIR
Sbjct: 88  WKPPSKG-TVKLNVDAAFDVRNQRGGVGVLVRDDRNSILAALISSHNVNSPLLAEICAIR 147

Query: 63  EGLRLATR 69
           E +RLA R
Sbjct: 148 EAVRLAER 154

The following BLAST results are available for this feature:

BLAST of MS019014 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155289.1	4.9e-41	98.89	uncharacterized protein LOC111022426 [Momordica charantia]	[more]
XP_022158489.1	1.9e-32	86.67	uncharacterized protein LOC111024968 [Momordica charantia]	[more]
XP_042939527.1	4.7e-07	44.32	uncharacterized protein LOC122274566 [Carya illinoinensis]	[more]
KAG6693248.1	6.1e-07	38.89	hypothetical protein I3842_10G159900, partial [Carya illinoinensis]	[more]
KAG6693249.1	6.1e-07	38.89	hypothetical protein I3842_10G159900 [Carya illinoinensis]	[more]

BLAST of MS019014 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS019014 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPU1	2.4e-41	98.89	uncharacterized protein LOC111022426 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DZK3	9.1e-33	86.67	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
M8ARB3	6.0e-08	46.59	Casein kinase I-2-like protein OS=Aegilops tauschii OX=37682 GN=F775_24143 PE=4 ...	[more]
A0A7J6WZQ1	3.9e-07	32.56	RNase H domain-containing protein OS=Thalictrum thalictroides OX=46969 GN=FRX31_...	[more]
A0A6J1CK80	3.9e-07	52.94	uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]

BLAST of MS019014 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 15..87 e-value: 4.9E-11 score: 42.5
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 10..90 e-value: 8.8E-10 score: 40.7
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 2..87
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 2..87
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 15..87 e-value: 5.141E-14 score: 60.4056
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 12..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019014.1	MS019014.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity