MS019010 (gene) Bitter gourd (TR) v1

Overview


Name	MS019010
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold20: 674247 .. 674546 (-)
RNA-Seq Expression	MS019010
Synteny	MS019010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGTCGCCTCTTCTTGCAGAAATCAGATGCATCTTGGAGGGTCTGAAATTTGCAGCTGCTTCAAATTTCACCCATTTAGAAGTCGAATCTGATTCTTTGTTGGCAATTCAATTGATTCGAAATGAGATACACACAAGGGGAGATGAGCAAAATTGGGTGATGGAGATTCAAGCCCTAACCTGCTGCTTTGCCTTCATTTCCTTTTCCCATTCGTCCCGTCAATGCAATCGTGCTGCCCATGGTTTAGCAAAATGGGGGATCACTTCCCCTTCTGCCACATATGCTTGGCTGTTTAATTTC

mRNA sequence

TTGTCGCCTCTTCTTGCAGAAATCAGATGCATCTTGGAGGGTCTGAAATTTGCAGCTGCTTCAAATTTCACCCATTTAGAAGTCGAATCTGATTCTTTGTTGGCAATTCAATTGATTCGAAATGAGATACACACAAGGGGAGATGAGCAAAATTGGGTGATGGAGATTCAAGCCCTAACCTGCTGCTTTGCCTTCATTTCCTTTTCCCATTCGTCCCGTCAATGCAATCGTGCTGCCCATGGTTTAGCAAAATGGGGGATCACTTCCCCTTCTGCCACATATGCTTGGCTGTTTAATTTC

Coding sequence (CDS)

TTGTCGCCTCTTCTTGCAGAAATCAGATGCATCTTGGAGGGTCTGAAATTTGCAGCTGCTTCAAATTTCACCCATTTAGAAGTCGAATCTGATTCTTTGTTGGCAATTCAATTGATTCGAAATGAGATACACACAAGGGGAGATGAGCAAAATTGGGTGATGGAGATTCAAGCCCTAACCTGCTGCTTTGCCTTCATTTCCTTTTCCCATTCGTCCCGTCAATGCAATCGTGCTGCCCATGGTTTAGCAAAATGGGGGATCACTTCCCCTTCTGCCACATATGCTTGGCTGTTTAATTTC

Protein sequence

LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF

Homology

[Close]

BLAST of MS019010 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 207.2 bits (526), Expect = 6.5e-50
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1    LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALT 60
            LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALT
Sbjct: 1340 LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALT 1399

Query: 61   CCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF 101
            CCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF
Sbjct: 1400 CCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF 1439

[Close]

BLAST of MS019010 vs. NCBI nr
Match: XP_018818291.2 (uncharacterized protein LOC108989209 [Juglans regia] >KAF5454765.1 hypothetical protein F2P56_024405 [Juglans regia])

HSP 1 Score: 62.4 bits (150), Expect = 2.6e-06
Identity = 35/87 (40.23%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 3   PLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCC 62
           P+  E+  IL GL+F      +HL +ESDSLL +  ++++  +R  + N V +I+ L   
Sbjct: 89  PIEIEMLAILRGLRFCFPLGISHLVIESDSLLMVNEVQSDSISRSLQGNMVQQIKQLMLR 148

Query: 63  FAFISFSHSSRQCNRAAHGLAK--WGI 88
           F   S  H+SR  N AAHGLAK  W I
Sbjct: 149 FPCCSIQHNSRLGNGAAHGLAKLAWNI 175

[Close]

BLAST of MS019010 vs. NCBI nr
Match: MBA0743320.1 (hypothetical protein [Gossypium gossypioides])

HSP 1 Score: 61.6 bits (148), Expect = 4.4e-06
Identity = 28/83 (33.73%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 12  LEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHS 71
           ++ ++F A S F  +EVE DSL  I+ ++N +  +     +++  + L   F    F H+
Sbjct: 104 IQTIQFGAKSGFLRIEVEGDSLTVIKKLQNNVKDKSKISAYIVNAKRLKSNFVIYKFKHA 163

Query: 72  SRQCNRAAHGLAKWGITSPSATY 95
           SRQ NR AH L+K G+   + TY
Sbjct: 164 SRQTNRVAHNLSKEGLNEEANTY 186

[Close]

BLAST of MS019010 vs. NCBI nr
Match: XP_018822505.1 (uncharacterized protein LOC108992400 [Juglans regia] >KAF5451995.1 hypothetical protein F2P56_027040 [Juglans regia])

HSP 1 Score: 60.8 bits (146), Expect = 7.5e-06
Identity = 35/87 (40.23%), Postives = 49/87 (56.32%), Query Frame = 0


Query: 3   PLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCC 62
           P+  E+  IL GL+F      +HL +ESDSLL +  ++++  +R    N V +I+ L   
Sbjct: 103 PIEIEMLAILRGLQFCFPLGISHLVIESDSLLMVNEVQSDSISRSLHGNLVQQIKQLMLR 162

Query: 63  FAFISFSHSSRQCNRAAHGLAK--WGI 88
           F   S  H+SR  N AAHGLAK  W I
Sbjct: 163 FPCCSIQHNSRLGNGAAHGLAKLAWNI 189

[Close]

BLAST of MS019010 vs. NCBI nr
Match: XP_021727655.1 (uncharacterized protein LOC110694792 [Chenopodium quinoa])

HSP 1 Score: 59.3 bits (142), Expect = 2.2e-05
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 2   SPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTC 61
           SPL AE + I+ GL  A  +    LEVESD L  +++++  +  +   Q  V++  +LT 
Sbjct: 28  SPLEAEAQAIMFGLTLAYDAGLRRLEVESDCLQLVKMLQGSVREKSAAQLTVLDAISLTS 87

Query: 62  CFAFISFSHSSRQCNRAAHGLAK 85
            F + SF+   R CN+AAH +AK
Sbjct: 88  IFEYCSFAFCPRICNKAAHLIAK 110

[Close]

BLAST of MS019010 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.1e-50
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1    LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALT 60
            LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALT
Sbjct: 1340 LSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALT 1399

Query: 61   CCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF 101
            CCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF
Sbjct: 1400 CCFAFISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF 1439

[Close]

BLAST of MS019010 vs. ExPASy TrEMBL
Match: A0A2I4EFW5 (uncharacterized protein LOC108989209 OS=Juglans regia OX=51240 GN=LOC108989209 PE=4 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-06
Identity = 35/87 (40.23%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 3   PLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCC 62
           P+  E+  IL GL+F      +HL +ESDSLL +  ++++  +R  + N V +I+ L   
Sbjct: 89  PIEIEMLAILRGLRFCFPLGISHLVIESDSLLMVNEVQSDSISRSLQGNMVQQIKQLMLR 148

Query: 63  FAFISFSHSSRQCNRAAHGLAK--WGI 88
           F   S  H+SR  N AAHGLAK  W I
Sbjct: 149 FPCCSIQHNSRLGNGAAHGLAKLAWNI 175

[Close]

BLAST of MS019010 vs. ExPASy TrEMBL
Match: A0A7J9C4B9 (RNase H domain-containing protein OS=Gossypium gossypioides OX=34282 GN=Gogos_006014 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-06
Identity = 28/83 (33.73%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 12  LEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHS 71
           ++ ++F A S F  +EVE DSL  I+ ++N +  +     +++  + L   F    F H+
Sbjct: 104 IQTIQFGAKSGFLRIEVEGDSLTVIKKLQNNVKDKSKISAYIVNAKRLKSNFVIYKFKHA 163

Query: 72  SRQCNRAAHGLAKWGITSPSATY 95
           SRQ NR AH L+K G+   + TY
Sbjct: 164 SRQTNRVAHNLSKEGLNEEANTY 186

[Close]

BLAST of MS019010 vs. ExPASy TrEMBL
Match: A0A2I4ESY4 (uncharacterized protein LOC108992400 OS=Juglans regia OX=51240 GN=LOC108992400 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.6e-06
Identity = 35/87 (40.23%), Postives = 49/87 (56.32%), Query Frame = 0


Query: 3   PLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCC 62
           P+  E+  IL GL+F      +HL +ESDSLL +  ++++  +R    N V +I+ L   
Sbjct: 103 PIEIEMLAILRGLQFCFPLGISHLVIESDSLLMVNEVQSDSISRSLHGNLVQQIKQLMLR 162

Query: 63  FAFISFSHSSRQCNRAAHGLAK--WGI 88
           F   S  H+SR  N AAHGLAK  W I
Sbjct: 163 FPCCSIQHNSRLGNGAAHGLAKLAWNI 189

[Close]

BLAST of MS019010 vs. ExPASy TrEMBL
Match: A0A1U8NYQ0 (uncharacterized protein LOC107952360 OS=Gossypium hirsutum OX=3635 GN=LOC107952360 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 4.0e-05
Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 0


Query: 5   LAEIRCILEGLKFAAASNFTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFA 64
           +AE     +GL FA  + FT +EVE DS   I+ I  E   R D  + +++I+++   F 
Sbjct: 388 MAERVACYQGLLFAKETGFTTVEVEGDSRTVIEKINQEGFGRADLDSVILDIKSMGRFFH 447

Query: 65  FISFSHSSRQCNRAAHGLAKWGITSPSATYAWLFNF 101
            + F H  R+ NR AH +A+ GI     T+ W+ +F
Sbjct: 448 QLRFKHVRREANRVAHFIAREGICRSENTF-WMEDF 482

The following BLAST results are available for this feature:

BLAST of MS019010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158377.1	6.5e-50	100.00	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_018818291.2	2.6e-06	40.23	uncharacterized protein LOC108989209 [Juglans regia] >KAF5454765.1 hypothetical ...	[more]
MBA0743320.1	4.4e-06	33.73	hypothetical protein [Gossypium gossypioides]	[more]
XP_018822505.1	7.5e-06	40.23	uncharacterized protein LOC108992400 [Juglans regia] >KAF5451995.1 hypothetical ...	[more]
XP_021727655.1	2.2e-05	37.35	uncharacterized protein LOC110694792 [Chenopodium quinoa]	[more]

BLAST of MS019010 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS019010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DX30	3.1e-50	100.00	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A2I4EFW5	1.2e-06	40.23	uncharacterized protein LOC108989209 OS=Juglans regia OX=51240 GN=LOC108989209 P...	[more]
A0A7J9C4B9	2.1e-06	33.73	RNase H domain-containing protein OS=Gossypium gossypioides OX=34282 GN=Gogos_00...	[more]
A0A2I4ESY4	3.6e-06	40.23	uncharacterized protein LOC108992400 OS=Juglans regia OX=51240 GN=LOC108992400 P...	[more]
A0A1U8NYQ0	4.0e-05	34.38	uncharacterized protein LOC107952360 OS=Gossypium hirsutum OX=3635 GN=LOC1079523...	[more]

BLAST of MS019010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 2..85 e-value: 1.1E-18 score: 67.3
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 1..88 score: 8.75456
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 2..88 e-value: 1.2E-12 score: 50.0
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 5..89
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 2..84 e-value: 9.5457E-19 score: 72.732
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 4..90

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS019010.1	MS019010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity