MS019003 (gene) Bitter gourd (TR) v1

Overview
NameMS019003
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionHeat shock protein DnaJ with tetratricopeptide repeat
Locationscaffold20: 603439 .. 606309 (+)
RNA-Seq ExpressionMS019003
SyntenyMS019003
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGTACGTGAGGGACGCCAGGTGCCTAATCGGCAGCGGAGGAGAGAACGATGTCGTTTCCGCCTTGAATCTTCTCGACGCGGCTCTGGTTCTTTCACCGAGGTGCGAGGACGCGCTGGAGCTCAGGGCTCGGTCGCTGCTGTATCTTCGGCGGTACAAGGACGTCGCCGATATGCTTCAGGAGTACCTGCCGAGTATGAGGACGGCGACCGGCGCCGGCGACGAGTCAGGATCGGACAACTCCTCGCAGCAATTTTCGAGAGAGCAAACGAAACTGCTCTCTCCTTCTTCGTCTTCGTCTTCGTCTTCGTCTTCATCTTCGTCTTCTTCGTTCTCTGAGTTGCAGGATCGCGACTCGTCGTTCAAGTGCTTCTCCGTCGCCGACTTGAAGAAGAAGATCATGGCCGGCCTGCTCTGTAAAAATTGCGACAAACAAAGTCAATGGAGGTAATTTTTCTTTACTTTTGTTTTTATTTTCTTCCTTCTTGAAATTGAAAATGATTCCTTCTAATCGTGAGCGTTTTGGCTATTTTTGGAAACATTTTTTCTTATAGTTTAAAAGTATTTAAATATGCCAAGAGCGAAGACACTTACTCTTAGTTAAATATTTAAATATACTAATTGCAAGAAAATGAGAACTTGTAATATTCAACACAAAAATATGTTTATTAGCCAGAAACTATATTGTACTTATCATCATTAGTTCCATGATAAAGTAATATATATACAGGAAAACAGATATTTAAATTTTTGTTGGTTGTCAACTGTAAACATTGTTTCAACATTTTGTTAATATACATAAAAATTATTAACAGAAACAAAATTGAGTAAAAATTGAAAATACAAATACTATATTTAGACCCTAGTAGTATCTTGTTACTTATCCTAAAGCATTTAATGGTAGAATTCTCATAAATAAGGACTTAAGTATTTTCTAAAAAAAAAAGATCTATTTTTACTTTGTGCATGTGTTGTGTCAATTTTTTTACTAATGGTTGTACGGAACCACTTAATATTATTAATATTTTTATCCCCAAAACTATAAATATATTTTTCTGGGTAAAAACAAGCACTATCTTAGACCAAAGGGGGTTATGTTAGATTAAAAACTTATTTTATTTATTATTCTTTCGACTTTTGTAATTCTTTCGGTTTTATACTTAAAAAATGTAATAATTTTTTTTTCTTCAAATGAACATAATATTGGTAAAATCAAGACAAAATGAATAAATACCTAATTTGACCGTCTTGTATTTTGTACACATCAAAGAGTCCATCAACATGCATTCTTTTCTCTATGTAGACTAATATTAAATGCTTTCTTTTATCAGAAAGTGTATGAACTTTTTTTTTAACTCAAACACATAATTTTTTATATTTTAAATTTAAAAAAAATATAAGAAGAGGGTAGAATAAATCTTTTCAAAAAATACAATAACAAAAAAGNACTTGTTTCGAAAAAAAAATTAGCTATTGAACTTTTAATTGTGTCTAACAAGTCTCTAATCTATTAGAAATTTTAAACTATGTGCAATTTTAAAGTTTAGAAACTTATTAGATACCATCTAAAAATTAAATATATATTATTGCTATTTTGATGTTAAGGAGTAAAAAATTTAAAAACAAGTTGTACATGTTTTTTACATGGAGTTAAATCAGTTTCACTCGCTTGGGTTTCCACAGATACATAGTATTGGGCCAGGCCTGCTGTCATCTGGGCCTATTAGAGGACGCCATGGTTCTCCTCCAGACCGGCAAACGGCTCGCCACTGCCGCCCTACGCCGGGAAAGCACTTTCCGGTCCGACGACCGCTTCCCCCTCCCCAATTTCCCAATTCTGTTCACCTCCGGCCACCCCCATCCCCTCACCGAATCCGAGACCATCTCCTCCCTCCTCACCCATGTGAAGCTCCTCCTCCGCCGCCGCACCGCCGCCCTCGCCGCCCTCGACGCCGGCCTCCACTCCGAGGCCATCCGCCACTTCACCAAGATCCTCGATGGCCGCCGCCCCGCCCCTCAAACCTTCCTCGCCGAGTGCTTCATGCACCGCTCCTCCGCCTACCGCTCCGCCGGCCGGATCGCCGACTCCATCGCCGACTGCAATTGGACCCTCACCCTCGACCCGACCTGCATCCAAGCCCTCGAAACCCGAGCCGAGCTCTTCGAATCCATCGGATGCCTCCCCGATTGCCTCCACGATCTCGAACACCTTAAACTCCTCTACAATTCAATCTTAAGAGACCGGAAACTCCCCGGTCCGGCGTGGAAACGCCACAATGTGCGGTACAGAGAAATCCCCGGGAAGCTCTGTGCGCTAACGGCGAGGATTCAAAAAGTGAAGCAGAGAGTTGCTTCCGGCGAAAATGGTAACGTCGATTACTATAGTTTGATCGGATTGCGACGTGGGTGTTCGAGATCTGAGCTCCAGAGAGCCCATTTGTTGCTCTGTTTGAAGCACACGCCCGACAAGGCCACCGGATTTTTAGACAGATGCGAGTTGGCCGATGATTTTCATCTCGATTCGATTAGAGACAGAGCGAAAATGTCGGCGTTGTTGCTGTATAGGCTGCTGCAGAAGGGGTATTCCAGTATTATGACGGCGATTATGGATGAGGAAGCGGCGGAGAAGCAGAGGAAGAGAGCGGCGGCGGCGGCGGCGCTGCAATCAGCTCCGGCGATCCAATTGCAGCACCAGGAAGATGGTGTTGATGAAATGGAATCGAAATCGGTGGAGATTTCGAGTCATAATAATAATAATAGTAAAGTGGTTTATCAAGGGGTGTTCTGCCGGGATCTGGCCGTCGTCGGGAATCTGCTGTCGCAGGTTGGATTTGGCCGTCCGATTGCGGTGAAGTATGAGGCGTTGAGCTGC

mRNA sequence

AGGTACGTGAGGGACGCCAGGTGCCTAATCGGCAGCGGAGGAGAGAACGATGTCGTTTCCGCCTTGAATCTTCTCGACGCGGCTCTGGTTCTTTCACCGAGGTGCGAGGACGCGCTGGAGCTCAGGGCTCGGTCGCTGCTGTATCTTCGGCGGTACAAGGACGTCGCCGATATGCTTCAGGAGTACCTGCCGAGTATGAGGACGGCGACCGGCGCCGGCGACGAGTCAGGATCGGACAACTCCTCGCAGCAATTTTCGAGAGAGCAAACGAAACTGCTCTCTCCTTCTTCGTCTTCGTCTTCGTCTTCGTCTTCATCTTCGTCTTCTTCGTTCTCTGAGTTGCAGGATCGCGACTCGTCGTTCAAGTGCTTCTCCGTCGCCGACTTGAAGAAGAAGATCATGGCCGGCCTGCTCTGTAAAAATTGCGACAAACAAAGTCAATGGAGATACATAGTATTGGGCCAGGCCTGCTGTCATCTGGGCCTATTAGAGGACGCCATGGTTCTCCTCCAGACCGGCAAACGGCTCGCCACTGCCGCCCTACGCCGGGAAAGCACTTTCCGGTCCGACGACCGCTTCCCCCTCCCCAATTTCCCAATTCTGTTCACCTCCGGCCACCCCCATCCCCTCACCGAATCCGAGACCATCTCCTCCCTCCTCACCCATGTGAAGCTCCTCCTCCGCCGCCGCACCGCCGCCCTCGCCGCCCTCGACGCCGGCCTCCACTCCGAGGCCATCCGCCACTTCACCAAGATCCTCGATGGCCGCCGCCCCGCCCCTCAAACCTTCCTCGCCGAGTGCTTCATGCACCGCTCCTCCGCCTACCGCTCCGCCGGCCGGATCGCCGACTCCATCGCCGACTGCAATTGGACCCTCACCCTCGACCCGACCTGCATCCAAGCCCTCGAAACCCGAGCCGAGCTCTTCGAATCCATCGGATGCCTCCCCGATTGCCTCCACGATCTCGAACACCTTAAACTCCTCTACAATTCAATCTTAAGAGACCGGAAACTCCCCGGTCCGGCGTGGAAACGCCACAATGTGCGGTACAGAGAAATCCCCGGGAAGCTCTGTGCGCTAACGGCGAGGATTCAAAAAGTGAAGCAGAGAGTTGCTTCCGGCGAAAATGGTAACGTCGATTACTATAGTTTGATCGGATTGCGACGTGGGTGTTCGAGATCTGAGCTCCAGAGAGCCCATTTGTTGCTCTGTTTGAAGCACACGCCCGACAAGGCCACCGGATTTTTAGACAGATGCGAGTTGGCCGATGATTTTCATCTCGATTCGATTAGAGACAGAGCGAAAATGTCGGCGTTGTTGCTGTATAGGCTGCTGCAGAAGGGGTATTCCAGTATTATGACGGCGATTATGGATGAGGAAGCGGCGGAGAAGCAGAGGAAGAGAGCGGCGGCGGCGGCGGCGCTGCAATCAGCTCCGGCGATCCAATTGCAGCACCAGGAAGATGGTGTTGATGAAATGGAATCGAAATCGGTGGAGATTTCGAGTCATAATAATAATAATAGTAAAGTGGTTTATCAAGGGGTGTTCTGCCGGGATCTGGCCGTCGTCGGGAATCTGCTGTCGCAGGTTGGATTTGGCCGTCCGATTGCGGTGAAGTATGAGGCGTTGAGCTGC

Coding sequence (CDS)

AGGTACGTGAGGGACGCCAGGTGCCTAATCGGCAGCGGAGGAGAGAACGATGTCGTTTCCGCCTTGAATCTTCTCGACGCGGCTCTGGTTCTTTCACCGAGGTGCGAGGACGCGCTGGAGCTCAGGGCTCGGTCGCTGCTGTATCTTCGGCGGTACAAGGACGTCGCCGATATGCTTCAGGAGTACCTGCCGAGTATGAGGACGGCGACCGGCGCCGGCGACGAGTCAGGATCGGACAACTCCTCGCAGCAATTTTCGAGAGAGCAAACGAAACTGCTCTCTCCTTCTTCGTCTTCGTCTTCGTCTTCGTCTTCATCTTCGTCTTCTTCGTTCTCTGAGTTGCAGGATCGCGACTCGTCGTTCAAGTGCTTCTCCGTCGCCGACTTGAAGAAGAAGATCATGGCCGGCCTGCTCTGTAAAAATTGCGACAAACAAAGTCAATGGAGATACATAGTATTGGGCCAGGCCTGCTGTCATCTGGGCCTATTAGAGGACGCCATGGTTCTCCTCCAGACCGGCAAACGGCTCGCCACTGCCGCCCTACGCCGGGAAAGCACTTTCCGGTCCGACGACCGCTTCCCCCTCCCCAATTTCCCAATTCTGTTCACCTCCGGCCACCCCCATCCCCTCACCGAATCCGAGACCATCTCCTCCCTCCTCACCCATGTGAAGCTCCTCCTCCGCCGCCGCACCGCCGCCCTCGCCGCCCTCGACGCCGGCCTCCACTCCGAGGCCATCCGCCACTTCACCAAGATCCTCGATGGCCGCCGCCCCGCCCCTCAAACCTTCCTCGCCGAGTGCTTCATGCACCGCTCCTCCGCCTACCGCTCCGCCGGCCGGATCGCCGACTCCATCGCCGACTGCAATTGGACCCTCACCCTCGACCCGACCTGCATCCAAGCCCTCGAAACCCGAGCCGAGCTCTTCGAATCCATCGGATGCCTCCCCGATTGCCTCCACGATCTCGAACACCTTAAACTCCTCTACAATTCAATCTTAAGAGACCGGAAACTCCCCGGTCCGGCGTGGAAACGCCACAATGTGCGGTACAGAGAAATCCCCGGGAAGCTCTGTGCGCTAACGGCGAGGATTCAAAAAGTGAAGCAGAGAGTTGCTTCCGGCGAAAATGGTAACGTCGATTACTATAGTTTGATCGGATTGCGACGTGGGTGTTCGAGATCTGAGCTCCAGAGAGCCCATTTGTTGCTCTGTTTGAAGCACACGCCCGACAAGGCCACCGGATTTTTAGACAGATGCGAGTTGGCCGATGATTTTCATCTCGATTCGATTAGAGACAGAGCGAAAATGTCGGCGTTGTTGCTGTATAGGCTGCTGCAGAAGGGGTATTCCAGTATTATGACGGCGATTATGGATGAGGAAGCGGCGGAGAAGCAGAGGAAGAGAGCGGCGGCGGCGGCGGCGCTGCAATCAGCTCCGGCGATCCAATTGCAGCACCAGGAAGATGGTGTTGATGAAATGGAATCGAAATCGGTGGAGATTTCGAGTCATAATAATAATAATAGTAAAGTGGTTTATCAAGGGGTGTTCTGCCGGGATCTGGCCGTCGTCGGGAATCTGCTGTCGCAGGTTGGATTTGGCCGTCCGATTGCGGTGAAGTATGAGGCGTTGAGCTGC

Protein sequence

RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQEYLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDSSFKCFSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATAALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAVKYEALSC
Homology
BLAST of MS019003 vs. NCBI nr
Match: XP_038906072.1 (uncharacterized protein LOC120091962 [Benincasa hispida])

HSP 1 Score: 817.0 bits (2109), Expect = 9.7e-233
Identity = 446/554 (80.51%), Postives = 477/554 (86.10%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+CLI +G ENDVVSALNLLDAALVL P  E ALEL+ARSLLYLRRYKDVADMLQ
Sbjct: 26  RYVKDAKCLIAAGAENDVVSALNLLDAALVLVPGYEQALELKARSLLYLRRYKDVADMLQ 85

Query: 61  EYLPSMRTAT--GAGDESGSDNSSQQFSREQTKLLSPSSSSSSS------SSSSSSSSFS 120
           EYLP M+ AT  G  + SGS+NSSQQFSREQ KLL+ SSSSSSS      SSSS SSS S
Sbjct: 86  EYLPCMKMATKSGGDESSGSENSSQQFSREQAKLLASSSSSSSSCSSSSCSSSSCSSSLS 145

Query: 121 ELQDRDSSFKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQ 180
           ELQDRDSSFKC FSVADLK+KIMAGLLCK  + +S+WRY+VLGQA CHLGLLEDAMVLLQ
Sbjct: 146 ELQDRDSSFKCFFSVADLKRKIMAGLLCKCRENESRWRYVVLGQASCHLGLLEDAMVLLQ 205

Query: 181 TGKRLATAALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRT 240
           TGKRLATAA RREST RSDD F L NFPIL TS       ES+T ++LL+HVKLLLRRRT
Sbjct: 206 TGKRLATAASRRESTGRSDDGFSLHNFPILPTS-----TAESDTTAALLSHVKLLLRRRT 265

Query: 241 AALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWT 300
           AA+AAL+AGL SEAIR FTKILDGRRPAPQTFLA+CF+HRSSA+R+AGR ADSIADCN T
Sbjct: 266 AAIAALEAGLPSEAIRLFTKILDGRRPAPQTFLAQCFLHRSSAFRAAGRTADSIADCNRT 325

Query: 301 LTLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYR 360
           LTLDPTCIQALETRA LFESI CLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNV+YR
Sbjct: 326 LTLDPTCIQALETRAHLFESIRCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVQYR 385

Query: 361 EIPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDK 420
           EIPGKLCALT RIQK+KQRVASGE  NVDYY LIGLRRGCSRSEL+RAHLLLCLKH PDK
Sbjct: 386 EIPGKLCALTTRIQKLKQRVASGETCNVDYYGLIGLRRGCSRSELERAHLLLCLKHAPDK 445

Query: 421 ATGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAA 480
           ATGFL+RCEL DDF LDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKR AA
Sbjct: 446 ATGFLERCELGDDFDLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRTAA 505

Query: 481 AAALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVG 540
              +Q     Q Q  E GVDEME K  EI   N +++  VYQGVFCRDLAVVGNLLSQVG
Sbjct: 506 VIQMQQ----QQQSHEGGVDEMELKLEEIPGSNLSHNTAVYQGVFCRDLAVVGNLLSQVG 565

Query: 541 FGRPIAVKYEALSC 546
           FGRPIAVKYEALSC
Sbjct: 566 FGRPIAVKYEALSC 570

BLAST of MS019003 vs. NCBI nr
Match: XP_008454300.1 (PREDICTED: uncharacterized protein LOC103494740 [Cucumis melo])

HSP 1 Score: 816.2 bits (2107), Expect = 1.7e-232
Identity = 440/546 (80.59%), Postives = 475/546 (87.00%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+ LIG+GGEN+VVSALNLLDAALVL+PR E ALEL+ARSLLYLRRYKDVADMLQ
Sbjct: 29  RYVKDAKYLIGAGGENEVVSALNLLDAALVLAPRYEQALELKARSLLYLRRYKDVADMLQ 88

Query: 61  EYLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDSS 120
           EYLPSMR  +G  + SGSDNSSQQFSREQ KLL   SSSSSSSS+ SSSS SELQDRDSS
Sbjct: 89  EYLPSMR--SGGDESSGSDNSSQQFSREQAKLL--VSSSSSSSSTCSSSSLSELQDRDSS 148

Query: 121 FKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATA 180
           FKC FSVADL++KIMAGLLC  C+ +S+WRY+VLGQA CHLGLLEDAMVLLQTGKRLATA
Sbjct: 149 FKCFFSVADLRRKIMAGLLCNCCENESRWRYVVLGQASCHLGLLEDAMVLLQTGKRLATA 208

Query: 181 ALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALDA 240
           A RREST RSDD F L NFPI+     P    ES+T ++LL+HVKLLLRRRTAA+AAL+A
Sbjct: 209 ASRRESTSRSDDGFSLNNFPIV-----PTTTVESDTTAALLSHVKLLLRRRTAAIAALEA 268

Query: 241 GLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTCI 300
           GL SEAIR FTKILDGRRP PQTFLA+CF+HRSSA+R+AGR ADSIADCN TLTLDPTCI
Sbjct: 269 GLPSEAIRLFTKILDGRRPVPQTFLAQCFLHRSSAFRAAGRTADSIADCNRTLTLDPTCI 328

Query: 301 QALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCA 360
           QALETRA LFESI CL DCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCA
Sbjct: 329 QALETRAHLFESIHCLVDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCA 388

Query: 361 LTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDRC 420
           LTARIQK+KQRVASGE  NVDYY LIGLRRGCSRSEL+RAHLLLCLKH PDKATGFL+RC
Sbjct: 389 LTARIQKLKQRVASGETCNVDYYGLIGLRRGCSRSELERAHLLLCLKHAPDKATGFLERC 448

Query: 421 ELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSAP 480
           EL DDF LDS+RD+AKMSALLLYRLLQKGYSSIMT IMDEEAAEKQRKR  A   +Q   
Sbjct: 449 ELGDDFDLDSVRDKAKMSALLLYRLLQKGYSSIMTTIMDEEAAEKQRKRTTAVIQMQQ-- 508

Query: 481 AIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAVK 540
             Q Q  E GVDEME KS +I   N N++  VYQGVFCRDLAVVGNLLSQVGFGRPIAVK
Sbjct: 509 --QQQSHEGGVDEMELKSEDIVGSNLNHNTSVYQGVFCRDLAVVGNLLSQVGFGRPIAVK 561

Query: 541 YEALSC 546
           YEALSC
Sbjct: 569 YEALSC 561

BLAST of MS019003 vs. NCBI nr
Match: KAA0044409.1 (Heat shock protein DnaJ with tetratricopeptide repeat isoform 1 [Cucumis melo var. makuwa] >TYK29536.1 Heat shock protein DnaJ with tetratricopeptide repeat isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 813.9 bits (2101), Expect = 8.2e-232
Identity = 439/545 (80.55%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 2   YVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQE 61
           YV+DA+ LIG+GGEN+VVSALNLLDAALVL+PR E ALEL+ARSLLYLRRYKDVADMLQE
Sbjct: 20  YVKDAKYLIGAGGENEVVSALNLLDAALVLAPRYEQALELKARSLLYLRRYKDVADMLQE 79

Query: 62  YLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDSSF 121
           YLPSMR  +G  + SGSDNSSQQFSREQ KLL   SSSSSSSS+ SSSS SELQDRDSSF
Sbjct: 80  YLPSMR--SGGDESSGSDNSSQQFSREQAKLL--VSSSSSSSSTCSSSSLSELQDRDSSF 139

Query: 122 KC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATAA 181
           KC FSVADL++KIMAGLLC  C+ +S+WRY+VLGQA CHLGLLEDAMVLLQTGKRLATAA
Sbjct: 140 KCFFSVADLRRKIMAGLLCNCCENESRWRYVVLGQASCHLGLLEDAMVLLQTGKRLATAA 199

Query: 182 LRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALDAG 241
            RREST RSDD F L NFPI+     P    ES+T ++LL+HVKLLLRRRTAA+AAL+AG
Sbjct: 200 SRRESTSRSDDGFSLNNFPIV-----PTTTVESDTTAALLSHVKLLLRRRTAAIAALEAG 259

Query: 242 LHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTCIQ 301
           L SEAIR FTKILDGRRP PQTFLA+CF+HRSSA+R+AGR ADSIADCN TLTLDPTCIQ
Sbjct: 260 LPSEAIRLFTKILDGRRPVPQTFLAQCFLHRSSAFRAAGRTADSIADCNRTLTLDPTCIQ 319

Query: 302 ALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL 361
           ALETRA LFESI CL DCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL
Sbjct: 320 ALETRAHLFESIHCLVDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL 379

Query: 362 TARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDRCE 421
           TARIQK+KQRVASGE  NVDYY LIGLRRGCSRSEL+RAHLLLCLKH PDKATGFL+RCE
Sbjct: 380 TARIQKLKQRVASGETCNVDYYGLIGLRRGCSRSELERAHLLLCLKHAPDKATGFLERCE 439

Query: 422 LADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSAPA 481
           L DDF LDS+RD+AKMSALLLYRLLQKGYSSIMT IMDEEAAEKQRKR  A   +Q    
Sbjct: 440 LGDDFDLDSVRDKAKMSALLLYRLLQKGYSSIMTTIMDEEAAEKQRKRTTAVIQMQQ--- 499

Query: 482 IQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAVKY 541
            Q Q  E GVDEME KS +I   N N++  VYQGVFCRDLAVVGNLLSQVGFGRPIAVKY
Sbjct: 500 -QQQSHEGGVDEMELKSEDIVGSNLNHNTSVYQGVFCRDLAVVGNLLSQVGFGRPIAVKY 551

Query: 542 EALSC 546
           EALSC
Sbjct: 560 EALSC 551

BLAST of MS019003 vs. NCBI nr
Match: XP_022998609.1 (uncharacterized protein LOC111493197 [Cucurbita maxima])

HSP 1 Score: 812.8 bits (2098), Expect = 1.8e-231
Identity = 439/555 (79.10%), Postives = 479/555 (86.31%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+CLIG+G ENDVVSALNLLDAAL+L+PR E ALEL+ARSLL LRRYKDV DMLQ
Sbjct: 26  RYVKDAKCLIGAGRENDVVSALNLLDAALLLAPRFEQALELKARSLLCLRRYKDVVDMLQ 85

Query: 61  EYLPSMRT--ATGAGDE--SGSDNSSQQFSREQTKLLSPSSSSSSSSSSSS-----SSSF 120
           EYLPSMRT  +   GD+  SGS+NSSQQFSREQ KL + SSSSSSSSSSSS     SSS 
Sbjct: 86  EYLPSMRTTLSRSGGDDSSSGSENSSQQFSREQAKLFASSSSSSSSSSSSSSLSYCSSSL 145

Query: 121 SELQDRDSSFKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLL 180
            ELQ  DSSF C FSV+DLK+KI+AGLLCK C+K+SQWRY+VLGQA CHLGLLEDAMVLL
Sbjct: 146 LELQSHDSSFNCFFSVSDLKRKIIAGLLCKRCEKESQWRYVVLGQAACHLGLLEDAMVLL 205

Query: 181 QTGKRLATAALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRR 240
           QTGKRLATAA RREST RS+D F LP+FP+L     P   TE++T ++LL+HVKLLLRRR
Sbjct: 206 QTGKRLATAASRRESTGRSEDGFSLPSFPVL-----PTTTTEADTTAALLSHVKLLLRRR 265

Query: 241 TAALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNW 300
           TAALAALDAGL SEAIR FTKIL+GRRPAPQTFLA+CF+HRSSAYR+AGR ADSIADCN 
Sbjct: 266 TAALAALDAGLPSEAIRLFTKILEGRRPAPQTFLAQCFLHRSSAYRAAGRTADSIADCNR 325

Query: 301 TLTLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRY 360
           TL LDPTCIQALETRA LFESI CLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNV+Y
Sbjct: 326 TLILDPTCIQALETRAHLFESIRCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVQY 385

Query: 361 REIPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPD 420
           REIPGKLCALTA+IQK+KQRVASGE  NVDYY LIGL+RGCSRS+LQR HLLLCLKHTPD
Sbjct: 386 REIPGKLCALTAKIQKLKQRVASGEKNNVDYYGLIGLQRGCSRSDLQRGHLLLCLKHTPD 445

Query: 421 KATGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAA 480
           KATGFLDRCEL +DF ++SIRDRAKMS LLLYRLLQKGYSSIMT IMDEEAAEKQRKRAA
Sbjct: 446 KATGFLDRCELGNDFDMESIRDRAKMSGLLLYRLLQKGYSSIMTTIMDEEAAEKQRKRAA 505

Query: 481 AAAALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQV 540
           A  A Q     Q Q     +DE+ESK  EIS  N +N+K VYQGVFCRDLAVVGNLLSQV
Sbjct: 506 AIQAQQQ----QQQTHAKAIDEIESKQEEISGSNLSNNKSVYQGVFCRDLAVVGNLLSQV 565

Query: 541 GFGRPIAVKYEALSC 546
           GFGRPIAVKYEALSC
Sbjct: 566 GFGRPIAVKYEALSC 571

BLAST of MS019003 vs. NCBI nr
Match: KAG7036648.1 (hypothetical protein SDJN02_00268 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 812.4 bits (2097), Expect = 2.4e-231
Identity = 438/553 (79.20%), Postives = 478/553 (86.44%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+CLIG+G ENDVVSALNLLDAAL+L+PR E ALEL+ARSLL LRRYKDV DMLQ
Sbjct: 26  RYVKDAKCLIGAGRENDVVSALNLLDAALILAPRFEQALELKARSLLCLRRYKDVVDMLQ 85

Query: 61  EYLPSMRT--ATGAGDE--SGSDNSSQQFSREQTKLLSPSSSSSSSSSSS---SSSSFSE 120
           EYLPSMRT  +   GD+  SGS+NSSQQFSREQ KLL+ SSSSSSSSSSS    SSS  E
Sbjct: 86  EYLPSMRTTLSRSGGDDSSSGSENSSQQFSREQAKLLASSSSSSSSSSSSLSYCSSSLLE 145

Query: 121 LQDRDSSFKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQT 180
           LQ+ DSSF C FSVADLK+KIMAGLLCK C+K+SQWRY+VLGQA CHLGLLEDAMVLLQT
Sbjct: 146 LQNHDSSFNCFFSVADLKRKIMAGLLCKRCEKESQWRYVVLGQAACHLGLLEDAMVLLQT 205

Query: 181 GKRLATAALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTA 240
           GKRLATAA RREST RS+D F LP+FP+L     P   TE++T ++LL+HVKLLLRRRTA
Sbjct: 206 GKRLATAASRRESTGRSEDGFSLPSFPVL-----PTTTTEADTTAALLSHVKLLLRRRTA 265

Query: 241 ALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTL 300
           ALAALDAGL SEAIR FTKIL+GRRPAPQTFLA+CF+HRSSAYR+AGR ADSIADCN TL
Sbjct: 266 ALAALDAGLPSEAIRLFTKILEGRRPAPQTFLAQCFLHRSSAYRAAGRTADSIADCNRTL 325

Query: 301 TLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYRE 360
            LDPTCIQALETRA LFESI CLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNV+YRE
Sbjct: 326 ILDPTCIQALETRAHLFESIRCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVQYRE 385

Query: 361 IPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKA 420
           IPGKLCALTARIQK+KQRVASGE  NVDYY LIGL+RGC RS+L+RAHLLLCLKHTPDKA
Sbjct: 386 IPGKLCALTARIQKLKQRVASGEKNNVDYYGLIGLQRGCLRSDLERAHLLLCLKHTPDKA 445

Query: 421 TGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAA 480
           TGFLDRCEL +DF ++SIRDRAKMS L LYRLLQKGYSSIMT IMDEEAAEKQRKRAAA 
Sbjct: 446 TGFLDRCELGNDFDMESIRDRAKMSGLFLYRLLQKGYSSIMTTIMDEEAAEKQRKRAAAI 505

Query: 481 AALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGF 540
              Q     Q Q     +DE+ESK  EIS  N+ ++K VYQGVFCRDLAVVGNLLSQVGF
Sbjct: 506 QTQQQQQ--QQQSHAKAIDEIESKQEEISGSNSTHNKSVYQGVFCRDLAVVGNLLSQVGF 565

Query: 541 GRPIAVKYEALSC 546
           GRPIAVKYEALSC
Sbjct: 566 GRPIAVKYEALSC 571

BLAST of MS019003 vs. ExPASy TrEMBL
Match: A0A1S3BXS6 (uncharacterized protein LOC103494740 OS=Cucumis melo OX=3656 GN=LOC103494740 PE=4 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 8.0e-233
Identity = 440/546 (80.59%), Postives = 475/546 (87.00%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+ LIG+GGEN+VVSALNLLDAALVL+PR E ALEL+ARSLLYLRRYKDVADMLQ
Sbjct: 29  RYVKDAKYLIGAGGENEVVSALNLLDAALVLAPRYEQALELKARSLLYLRRYKDVADMLQ 88

Query: 61  EYLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDSS 120
           EYLPSMR  +G  + SGSDNSSQQFSREQ KLL   SSSSSSSS+ SSSS SELQDRDSS
Sbjct: 89  EYLPSMR--SGGDESSGSDNSSQQFSREQAKLL--VSSSSSSSSTCSSSSLSELQDRDSS 148

Query: 121 FKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATA 180
           FKC FSVADL++KIMAGLLC  C+ +S+WRY+VLGQA CHLGLLEDAMVLLQTGKRLATA
Sbjct: 149 FKCFFSVADLRRKIMAGLLCNCCENESRWRYVVLGQASCHLGLLEDAMVLLQTGKRLATA 208

Query: 181 ALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALDA 240
           A RREST RSDD F L NFPI+     P    ES+T ++LL+HVKLLLRRRTAA+AAL+A
Sbjct: 209 ASRRESTSRSDDGFSLNNFPIV-----PTTTVESDTTAALLSHVKLLLRRRTAAIAALEA 268

Query: 241 GLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTCI 300
           GL SEAIR FTKILDGRRP PQTFLA+CF+HRSSA+R+AGR ADSIADCN TLTLDPTCI
Sbjct: 269 GLPSEAIRLFTKILDGRRPVPQTFLAQCFLHRSSAFRAAGRTADSIADCNRTLTLDPTCI 328

Query: 301 QALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCA 360
           QALETRA LFESI CL DCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCA
Sbjct: 329 QALETRAHLFESIHCLVDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCA 388

Query: 361 LTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDRC 420
           LTARIQK+KQRVASGE  NVDYY LIGLRRGCSRSEL+RAHLLLCLKH PDKATGFL+RC
Sbjct: 389 LTARIQKLKQRVASGETCNVDYYGLIGLRRGCSRSELERAHLLLCLKHAPDKATGFLERC 448

Query: 421 ELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSAP 480
           EL DDF LDS+RD+AKMSALLLYRLLQKGYSSIMT IMDEEAAEKQRKR  A   +Q   
Sbjct: 449 ELGDDFDLDSVRDKAKMSALLLYRLLQKGYSSIMTTIMDEEAAEKQRKRTTAVIQMQQ-- 508

Query: 481 AIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAVK 540
             Q Q  E GVDEME KS +I   N N++  VYQGVFCRDLAVVGNLLSQVGFGRPIAVK
Sbjct: 509 --QQQSHEGGVDEMELKSEDIVGSNLNHNTSVYQGVFCRDLAVVGNLLSQVGFGRPIAVK 561

Query: 541 YEALSC 546
           YEALSC
Sbjct: 569 YEALSC 561

BLAST of MS019003 vs. ExPASy TrEMBL
Match: A0A5D3E0U9 (Heat shock protein DnaJ with tetratricopeptide repeat isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001390 PE=4 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 4.0e-232
Identity = 439/545 (80.55%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 2   YVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQE 61
           YV+DA+ LIG+GGEN+VVSALNLLDAALVL+PR E ALEL+ARSLLYLRRYKDVADMLQE
Sbjct: 20  YVKDAKYLIGAGGENEVVSALNLLDAALVLAPRYEQALELKARSLLYLRRYKDVADMLQE 79

Query: 62  YLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDSSF 121
           YLPSMR  +G  + SGSDNSSQQFSREQ KLL   SSSSSSSS+ SSSS SELQDRDSSF
Sbjct: 80  YLPSMR--SGGDESSGSDNSSQQFSREQAKLL--VSSSSSSSSTCSSSSLSELQDRDSSF 139

Query: 122 KC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATAA 181
           KC FSVADL++KIMAGLLC  C+ +S+WRY+VLGQA CHLGLLEDAMVLLQTGKRLATAA
Sbjct: 140 KCFFSVADLRRKIMAGLLCNCCENESRWRYVVLGQASCHLGLLEDAMVLLQTGKRLATAA 199

Query: 182 LRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALDAG 241
            RREST RSDD F L NFPI+     P    ES+T ++LL+HVKLLLRRRTAA+AAL+AG
Sbjct: 200 SRRESTSRSDDGFSLNNFPIV-----PTTTVESDTTAALLSHVKLLLRRRTAAIAALEAG 259

Query: 242 LHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTCIQ 301
           L SEAIR FTKILDGRRP PQTFLA+CF+HRSSA+R+AGR ADSIADCN TLTLDPTCIQ
Sbjct: 260 LPSEAIRLFTKILDGRRPVPQTFLAQCFLHRSSAFRAAGRTADSIADCNRTLTLDPTCIQ 319

Query: 302 ALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL 361
           ALETRA LFESI CL DCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL
Sbjct: 320 ALETRAHLFESIHCLVDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL 379

Query: 362 TARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDRCE 421
           TARIQK+KQRVASGE  NVDYY LIGLRRGCSRSEL+RAHLLLCLKH PDKATGFL+RCE
Sbjct: 380 TARIQKLKQRVASGETCNVDYYGLIGLRRGCSRSELERAHLLLCLKHAPDKATGFLERCE 439

Query: 422 LADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSAPA 481
           L DDF LDS+RD+AKMSALLLYRLLQKGYSSIMT IMDEEAAEKQRKR  A   +Q    
Sbjct: 440 LGDDFDLDSVRDKAKMSALLLYRLLQKGYSSIMTTIMDEEAAEKQRKRTTAVIQMQQ--- 499

Query: 482 IQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAVKY 541
            Q Q  E GVDEME KS +I   N N++  VYQGVFCRDLAVVGNLLSQVGFGRPIAVKY
Sbjct: 500 -QQQSHEGGVDEMELKSEDIVGSNLNHNTSVYQGVFCRDLAVVGNLLSQVGFGRPIAVKY 551

Query: 542 EALSC 546
           EALSC
Sbjct: 560 EALSC 551

BLAST of MS019003 vs. ExPASy TrEMBL
Match: A0A6J1KES5 (uncharacterized protein LOC111493197 OS=Cucurbita maxima OX=3661 GN=LOC111493197 PE=4 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 8.9e-232
Identity = 439/555 (79.10%), Postives = 479/555 (86.31%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+CLIG+G ENDVVSALNLLDAAL+L+PR E ALEL+ARSLL LRRYKDV DMLQ
Sbjct: 26  RYVKDAKCLIGAGRENDVVSALNLLDAALLLAPRFEQALELKARSLLCLRRYKDVVDMLQ 85

Query: 61  EYLPSMRT--ATGAGDE--SGSDNSSQQFSREQTKLLSPSSSSSSSSSSSS-----SSSF 120
           EYLPSMRT  +   GD+  SGS+NSSQQFSREQ KL + SSSSSSSSSSSS     SSS 
Sbjct: 86  EYLPSMRTTLSRSGGDDSSSGSENSSQQFSREQAKLFASSSSSSSSSSSSSSLSYCSSSL 145

Query: 121 SELQDRDSSFKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLL 180
            ELQ  DSSF C FSV+DLK+KI+AGLLCK C+K+SQWRY+VLGQA CHLGLLEDAMVLL
Sbjct: 146 LELQSHDSSFNCFFSVSDLKRKIIAGLLCKRCEKESQWRYVVLGQAACHLGLLEDAMVLL 205

Query: 181 QTGKRLATAALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRR 240
           QTGKRLATAA RREST RS+D F LP+FP+L     P   TE++T ++LL+HVKLLLRRR
Sbjct: 206 QTGKRLATAASRRESTGRSEDGFSLPSFPVL-----PTTTTEADTTAALLSHVKLLLRRR 265

Query: 241 TAALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNW 300
           TAALAALDAGL SEAIR FTKIL+GRRPAPQTFLA+CF+HRSSAYR+AGR ADSIADCN 
Sbjct: 266 TAALAALDAGLPSEAIRLFTKILEGRRPAPQTFLAQCFLHRSSAYRAAGRTADSIADCNR 325

Query: 301 TLTLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRY 360
           TL LDPTCIQALETRA LFESI CLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNV+Y
Sbjct: 326 TLILDPTCIQALETRAHLFESIRCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVQY 385

Query: 361 REIPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPD 420
           REIPGKLCALTA+IQK+KQRVASGE  NVDYY LIGL+RGCSRS+LQR HLLLCLKHTPD
Sbjct: 386 REIPGKLCALTAKIQKLKQRVASGEKNNVDYYGLIGLQRGCSRSDLQRGHLLLCLKHTPD 445

Query: 421 KATGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAA 480
           KATGFLDRCEL +DF ++SIRDRAKMS LLLYRLLQKGYSSIMT IMDEEAAEKQRKRAA
Sbjct: 446 KATGFLDRCELGNDFDMESIRDRAKMSGLLLYRLLQKGYSSIMTTIMDEEAAEKQRKRAA 505

Query: 481 AAAALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQV 540
           A  A Q     Q Q     +DE+ESK  EIS  N +N+K VYQGVFCRDLAVVGNLLSQV
Sbjct: 506 AIQAQQQ----QQQTHAKAIDEIESKQEEISGSNLSNNKSVYQGVFCRDLAVVGNLLSQV 565

Query: 541 GFGRPIAVKYEALSC 546
           GFGRPIAVKYEALSC
Sbjct: 566 GFGRPIAVKYEALSC 571

BLAST of MS019003 vs. ExPASy TrEMBL
Match: A0A6J1G8X5 (uncharacterized protein LOC111451999 OS=Cucurbita moschata OX=3662 GN=LOC111451999 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.8e-231
Identity = 437/553 (79.02%), Postives = 477/553 (86.26%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DA+CLIG+G ENDVVSALNLLDAAL+L+PR E ALEL+ARSLL LRRYKDV DMLQ
Sbjct: 26  RYVKDAKCLIGAGRENDVVSALNLLDAALILAPRFEQALELKARSLLCLRRYKDVVDMLQ 85

Query: 61  EYLPSMRT--ATGAGDE--SGSDNSSQQFSREQTKLLSPSSSSSSSSSSS---SSSSFSE 120
           EYLPSMRT  +   GD+  SGS+NSSQQFSREQ KLL+ SS SSSSSSSS    SSS  E
Sbjct: 86  EYLPSMRTTLSRSGGDDSSSGSENSSQQFSREQAKLLASSSYSSSSSSSSLSYCSSSLLE 145

Query: 121 LQDRDSSFKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQT 180
           LQ+ DSSF C FSVADLK+KIMAGLLCK C+K+SQWRY+VLGQA CHLGLLEDAMVLLQT
Sbjct: 146 LQNHDSSFNCFFSVADLKRKIMAGLLCKRCEKESQWRYVVLGQAACHLGLLEDAMVLLQT 205

Query: 181 GKRLATAALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTA 240
           GKRLATAA RREST RS+D F LP+FP+L     P   TE++T ++LL+HVKLLLRRRTA
Sbjct: 206 GKRLATAASRRESTGRSEDGFSLPSFPVL-----PTTTTEADTTAALLSHVKLLLRRRTA 265

Query: 241 ALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTL 300
           ALAALDAGL SEAIR FTKIL+GRRPAPQTFLA+CF+HRSSAYR+AGR ADSIADCN TL
Sbjct: 266 ALAALDAGLPSEAIRLFTKILEGRRPAPQTFLAQCFLHRSSAYRAAGRTADSIADCNRTL 325

Query: 301 TLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYRE 360
            LDPTCIQALETRA LFESI CLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNV+YRE
Sbjct: 326 ILDPTCIQALETRAHLFESIRCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVQYRE 385

Query: 361 IPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKA 420
           IPGKLCALTARIQK+KQRVASGE  NVDYY LIGL+RGC RS+L+RAHLLLCLKHTPDKA
Sbjct: 386 IPGKLCALTARIQKLKQRVASGEKNNVDYYGLIGLQRGCLRSDLERAHLLLCLKHTPDKA 445

Query: 421 TGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAA 480
           TGFLDRCEL +DF ++SIRDRAKMS L LYRLLQKGYSSIMT IMDEEAAEKQRKRAAA 
Sbjct: 446 TGFLDRCELGNDFDMESIRDRAKMSGLFLYRLLQKGYSSIMTTIMDEEAAEKQRKRAAAI 505

Query: 481 AALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGF 540
              Q     Q Q     +DE+ESK  EIS  N+ ++K VYQGVFCRDLAVVGNLLSQVGF
Sbjct: 506 QTQQQQQ--QQQSHAKAIDEIESKQEEISGSNSTHNKSVYQGVFCRDLAVVGNLLSQVGF 565

Query: 541 GRPIAVKYEALSC 546
           GRPIAVKYEALSC
Sbjct: 566 GRPIAVKYEALSC 571

BLAST of MS019003 vs. ExPASy TrEMBL
Match: A0A0A0KT92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G003100 PE=4 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.9e-229
Identity = 437/547 (79.89%), Postives = 470/547 (85.92%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           RYV+DARCLI SGGEN+VVSALNLLDAALVL+PR E ALEL+ARSLLYLRRYKDVADMLQ
Sbjct: 26  RYVKDARCLIASGGENEVVSALNLLDAALVLAPRYEQALELKARSLLYLRRYKDVADMLQ 85

Query: 61  EYLPSMRTATGAGDESGSDN-SSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDS 120
           EYLPSMR  +G  + SGS+N SSQQFSREQ KLL    SSSSSSSS+ S S SELQD DS
Sbjct: 86  EYLPSMR--SGGDESSGSENYSSQQFSREQAKLL---VSSSSSSSSTCSISLSELQDHDS 145

Query: 121 SFKC-FSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLAT 180
           SFKC FSVADL++KIMAGLLC  CD +S+WRY+VLGQA CHLGLLEDAMVLLQTGKRLAT
Sbjct: 146 SFKCFFSVADLRRKIMAGLLCNCCDNESRWRYVVLGQASCHLGLLEDAMVLLQTGKRLAT 205

Query: 181 AALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALD 240
           AA RREST RSDD F L NFPIL     P    ES+T ++LL+HVKLLLRRRTAA+AAL+
Sbjct: 206 AASRRESTSRSDDGFSLNNFPIL-----PTTTVESDTTTALLSHVKLLLRRRTAAIAALE 265

Query: 241 AGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTC 300
           AGL SEAIR FTKILDGRRP PQTFLA+CF+HRSSA+R+AGR ADSIADCN TLTLDPTC
Sbjct: 266 AGLPSEAIRLFTKILDGRRPVPQTFLAQCFLHRSSAFRAAGRTADSIADCNRTLTLDPTC 325

Query: 301 IQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLC 360
           IQALETRA L+ESI  L DCLHDLEHLKLLYNSILRDRKLPGP WKRHNVRYREIPGKLC
Sbjct: 326 IQALETRAHLYESINYLADCLHDLEHLKLLYNSILRDRKLPGPVWKRHNVRYREIPGKLC 385

Query: 361 ALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDR 420
           ALTARIQK+KQRVASGE  NVDYY LIGLRRGCSRSEL+RAHLLLCLKH PDKATGFL+R
Sbjct: 386 ALTARIQKLKQRVASGETCNVDYYGLIGLRRGCSRSELERAHLLLCLKHAPDKATGFLER 445

Query: 421 CELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSA 480
           CEL DDF  DS+RD+AKMSAL LYRLLQKGYSSIMT+IMDEEAAEKQRKR  A   +Q  
Sbjct: 446 CELGDDFDPDSVRDKAKMSALFLYRLLQKGYSSIMTSIMDEEAAEKQRKRTTAVIQMQQ- 505

Query: 481 PAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAV 540
              Q Q QE GVDEME KS EI   N N++  VYQGVFCRDLAVVGNLLSQVGFGRPIAV
Sbjct: 506 ---QQQSQEGGVDEMELKSEEIVGSNLNHNTSVYQGVFCRDLAVVGNLLSQVGFGRPIAV 558

Query: 541 KYEALSC 546
           KYEALSC
Sbjct: 566 KYEALSC 558

BLAST of MS019003 vs. TAIR 10
Match: AT4G02100.1 (Heat shock protein DnaJ with tetratricopeptide repeat )

HSP 1 Score: 626.7 bits (1615), Expect = 1.7e-179
Identity = 344/559 (61.54%), Postives = 422/559 (75.49%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           +Y+++A+ LI S   NDV SALNLL++AL +SPR E ALEL+ARSLLYLRRYKDVADMLQ
Sbjct: 20  KYMKEAKSLIASQDPNDVKSALNLLESALSVSPRYELALELKARSLLYLRRYKDVADMLQ 79

Query: 61  EYLPSMRTATGAGDESGSDNSSQQF---SREQTKLLSPSSSSSSSSSSSSSSSFSELQDR 120
           +Y+PS++ A G G++SG  +S   F   SRE  KLL+   S                   
Sbjct: 80  DYIPSLKLA-GGGEDSGIGSSELSFTHSSRESVKLLNDLPSHH--------------HHH 139

Query: 121 DSSFKCFSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLA 180
           DSSFKCFSV+DLKKK+MAG L KNCD+Q QWRY+VLGQACCHLGL+EDAMVLLQTGKRLA
Sbjct: 140 DSSFKCFSVSDLKKKVMAG-LTKNCDEQGQWRYLVLGQACCHLGLMEDAMVLLQTGKRLA 199

Query: 181 TAALRRESTFRSDDRFPL----------PNFPILFTSGHPHPLTESETISSLLTHVKLLL 240
           TAA RR+S   SDD F L          P+  ++ ++  P     SE+I+ +L+H+KLLL
Sbjct: 200 TAAFRRQSICWSDDSFILFSSEDGGSSPPSSVVVTSASQP----RSESIAHVLSHIKLLL 259

Query: 241 RRRTAALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIAD 300
           RRR AALAALDAGL++E+IRHF+KILD RR APQ FLA+CFMHR+SAYRSAGRIA+SIAD
Sbjct: 260 RRRAAALAALDAGLYTESIRHFSKILDSRRGAPQGFLAQCFMHRASAYRSAGRIAESIAD 319

Query: 301 CNWTLTLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHN 360
           CN TL LDP+C+QALETRA L ES+ C PD LHDLEHLKLLYNSILRDRKLPGP WKRHN
Sbjct: 320 CNKTLALDPSCLQALETRAALLESVRCFPDSLHDLEHLKLLYNSILRDRKLPGPVWKRHN 379

Query: 361 VRYREIPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKH 420
           VRYREIPGKLC LT +IQ++KQ++A+GE GNVDYY+L+G+RR CSRSEL RA+LLL LKH
Sbjct: 380 VRYREIPGKLCVLTTKIQQLKQKIANGEIGNVDYYALMGIRRDCSRSELDRAYLLLNLKH 439

Query: 421 TPDKATGFLDRCELADD-FHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQR 480
            P+++  F+DR EL DD   LDS++DRA+MS LLLYRL+QKGYS + + I    A EKQR
Sbjct: 440 KPERSMSFIDRFELTDDEEELDSVKDRARMSTLLLYRLIQKGYSVVTSNI---AAVEKQR 499

Query: 481 KRAAAAAALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNL 540
           K  AAA A +S            + E   ++V ++ ++NNN+ VV +GVFCRDL VVG+L
Sbjct: 500 KAIAAAVATES--------HRSNIIETPIRAVAVAVNSNNNTNVV-KGVFCRDLTVVGSL 546

Query: 541 LSQVGFGRPIAVKYEALSC 546
           +++ GF +PI VKYEALSC
Sbjct: 560 IARTGFNQPIPVKYEALSC 546

BLAST of MS019003 vs. TAIR 10
Match: AT2G47440.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 616.3 bits (1588), Expect = 2.3e-176
Identity = 334/548 (60.95%), Postives = 408/548 (74.45%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           +Y++DA  L+ S   NDV SAL+LLDAAL +SPR E ALEL+ARSLL+LRR+KDVADMLQ
Sbjct: 24  KYIKDATTLMASEEANDVASALHLLDAALSISPRLETALELKARSLLFLRRFKDVADMLQ 83

Query: 61  EYLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDS- 120
           +Y+PS++      D+ G                     S+SS  SSSS   + L D  S 
Sbjct: 84  DYIPSLKL-----DDEG---------------------SASSQGSSSSDGINLLSDASSP 143

Query: 121 -SFKCFSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLAT 180
            SFKCFSV+DLKKK+MAG +CK CDK+ QWRY+VLGQACCHLGL+EDAMVLLQTGKRLA+
Sbjct: 144 GSFKCFSVSDLKKKVMAG-ICKKCDKEGQWRYVVLGQACCHLGLMEDAMVLLQTGKRLAS 203

Query: 181 AALRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALD 240
           A  RR S   SDD F L    +  +S    P  ESE  + LL H+KLLLRRR AA+AALD
Sbjct: 204 AEFRRRSICWSDDSFLL----LSESSSASSPPPESENFTHLLAHIKLLLRRRAAAIAALD 263

Query: 241 AGLHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTC 300
           AGL SE+IRHF+KI+DGRRPAPQ FLAEC+MHR++AYRSAGRIA++IADCN TL L+P+C
Sbjct: 264 AGLFSESIRHFSKIVDGRRPAPQGFLAECYMHRAAAYRSAGRIAEAIADCNKTLALEPSC 323

Query: 301 IQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLC 360
           IQALETRA L E++ C PD LHDLEHLKLLYN+ILRDRKLPGP WKRHNV+YREIPGKLC
Sbjct: 324 IQALETRAALLETVRCFPDSLHDLEHLKLLYNTILRDRKLPGPVWKRHNVKYREIPGKLC 383

Query: 361 ALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDR 420
            LT + QK+KQ++A+GE GNVDYY LIG+RRGC+RSEL RAHLLLCL++ PD+A+ F++R
Sbjct: 384 VLTTKTQKLKQKIANGETGNVDYYGLIGVRRGCTRSELDRAHLLLCLRYKPDRASSFIER 443

Query: 421 CELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAA-AAALQS 480
           CE  D   +DS+RDRAKMS+LLLYRL+QKGY+++ TAI+    AE+QRK A A A  ++ 
Sbjct: 444 CEFTDQNDVDSVRDRAKMSSLLLYRLIQKGYTAV-TAII----AEEQRKNAIAHAQKIEE 503

Query: 481 APAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIA 540
              ++           E+K V      N+N+   YQGVFCRDLA VGNLL++ GF  PI 
Sbjct: 504 RKPVEKSGSIKRTGNAETKPV------NSNA---YQGVFCRDLAAVGNLLTRAGFNHPIP 526

Query: 541 VKYEALSC 546
           VKYEAL+C
Sbjct: 564 VKYEALTC 526

BLAST of MS019003 vs. TAIR 10
Match: AT3G62570.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 579.3 bits (1492), Expect = 3.2e-165
Identity = 316/553 (57.14%), Postives = 403/553 (72.88%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           +Y++DAR L+ S  +NDV SA++LLDAAL +SPR E ALEL+ARSLL+LRR+KDV DMLQ
Sbjct: 24  KYIKDARSLMESEEQNDVASAIHLLDAALSISPRSETALELKARSLLFLRRFKDVVDMLQ 83

Query: 61  EYLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQD-RDS 120
           +Y+PS++ A    +E GS      +S E        SS SSSSS  S    S+    RDS
Sbjct: 84  DYIPSLKLA--VNEEDGS------YSYE-------GSSYSSSSSQLSRKLLSDSSPRRDS 143

Query: 121 SFKCFSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATA 180
           SFKCFSV+ LKKKIMAG +CKN D+  QWRY+VLGQACCHLGL+EDA+VLLQTGKRLAT 
Sbjct: 144 SFKCFSVSYLKKKIMAG-ICKNRDQDKQWRYVVLGQACCHLGLMEDALVLLQTGKRLATV 203

Query: 181 ALRRESTFRSDDRFPLPNFPILFTSGH------PHPLTESETISSLLTHVKLLLRRRTAA 240
             RR S   SDD   L       +S        P  ++E ET+++LL H K LLRRR+A 
Sbjct: 204 EFRRLSVSLSDDSVSLLLSESSSSSSSSSYAFPPRKVSECETVTNLLAHTKNLLRRRSAG 263

Query: 241 LAALDAGLHSEAIRHFTKILDG-RRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTL 300
            AA DAGL +++IRHF+KILDG RRPAPQ FLA+C+MHR++AY+SAG+IA++IADCN TL
Sbjct: 264 FAAFDAGLFADSIRHFSKILDGRRRPAPQGFLADCYMHRAAAYKSAGKIAEAIADCNKTL 323

Query: 301 TLDPTCIQALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYRE 360
            L+P+CI ALETRA L E++ CLPD LHDLEHLK+LYN+ILRDRKLPGP WKRHNV+YRE
Sbjct: 324 ALEPSCIHALETRATLLETVRCLPDSLHDLEHLKILYNTILRDRKLPGPPWKRHNVKYRE 383

Query: 361 IPGKLCALTARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKA 420
           IPGKLC LT + +K+K ++A+GE GNVDYY L+G+RRGC+RSEL RA+LLLCL+H PDKA
Sbjct: 384 IPGKLCELTTKSKKLKAKMANGEIGNVDYYGLVGVRRGCTRSELDRANLLLCLRHKPDKA 443

Query: 421 TGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAA 480
             F++RC+  D   + S++DRAKMS+LLLYRL+Q+GY+++  AI +EE    QRK+    
Sbjct: 444 LAFMERCDFFDQSEISSVKDRAKMSSLLLYRLIQRGYTALAAAIAEEE----QRKKMMVL 503

Query: 481 AALQSAPAIQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGF 540
             + +    + +  E       +   EI   N+N     YQGVFCRDLA VG+LLS+ GF
Sbjct: 504 TQMSTKTVEEHEPVEKSGSITLTDFAEIKPGNSN----AYQGVFCRDLAAVGSLLSRTGF 552

Query: 541 GRPIAVKYEALSC 546
            +PI +KY+A+SC
Sbjct: 564 NQPIPMKYDAISC 552

BLAST of MS019003 vs. TAIR 10
Match: AT1G02650.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 518.8 bits (1335), Expect = 5.1e-147
Identity = 287/538 (53.35%), Postives = 372/538 (69.14%), Query Frame = 0

Query: 1   RYVRDARCLIGSGGENDVVSALNLLDAALVLSPRCEDALELRARSLLYLRRYKDVADMLQ 60
           +Y+++A+ LI S   ND+VSAL+LL++ L +SP  E ALEL+ARSLLYLRR+KDVA +L 
Sbjct: 16  KYMKNAKDLITSQDPNDIVSALSLLNSTLSISPHHELALELKARSLLYLRRFKDVAVLLH 75

Query: 61  EYLPSMRTATGAGDESGSDNSSQQFSREQTKLLSPSSSSSSSSSSSSSSSFSELQDRDSS 120
            Y+PS+R      +E  S   +         LL PS S S                 DSS
Sbjct: 76  NYIPSLR----IDNEDVSSVFAASSELSSLMLLLPSGSPS----------------HDSS 135

Query: 121 FKCFSVADLKKKIMAGLLCKNCDKQSQWRYIVLGQACCHLGLLEDAMVLLQTGKRLATAA 180
           FKCFS + LKKK+MAG L  N   Q QWRY+VLGQAC HLGL++DA++LLQTGKRLATA 
Sbjct: 136 FKCFSYSYLKKKVMAG-LSNNSQVQGQWRYLVLGQACYHLGLMDDAIILLQTGKRLATAE 195

Query: 181 LRRESTFRSDDRFPLPNFPILFTSGHPHPLTESETISSLLTHVKLLLRRRTAALAALDAG 240
           LRRES   S+D F L       +   P P+TESE +S +L+  KL LRRRTAALAALDAG
Sbjct: 196 LRRESICWSEDSFNLST-----SESQPQPITESEIVSQMLSQTKLFLRRRTAALAALDAG 255

Query: 241 LHSEAIRHFTKILDGRRPAPQTFLAECFMHRSSAYRSAGRIADSIADCNWTLTLDPTCIQ 300
           L+SE+IRHF+KI+D RR APQ+FL  C + R+ AY+SAGRIADSIADCN  L L+P+CI+
Sbjct: 256 LYSESIRHFSKIIDSRRGAPQSFLVYCLIRRAFAYKSAGRIADSIADCNLILALEPSCIE 315

Query: 301 ALETRAELFESIGCLPDCLHDLEHLKLLYNSILRDRKLPGPAWKRHNVRYREIPGKLCAL 360
           ALETRAELF SI C PD LHDLEHLKLL+NSILRDR L GP WKRHNVRYREIPGKLC L
Sbjct: 316 ALETRAELFRSIRCFPDSLHDLEHLKLLFNSILRDRSLTGPVWKRHNVRYREIPGKLCVL 375

Query: 361 TARIQKVKQRVASGENGNVDYYSLIGLRRGCSRSELQRAHLLLCLKHTPDKATGFLDRCE 420
           T  I+++K+++ + ENGN DYYSL+G+ RGCSRSEL RA+LLL L++  +++   +DR +
Sbjct: 376 TTNIKQMKEKITNRENGNEDYYSLMGIERGCSRSELNRAYLLLNLRYKSERSMTSIDRFD 435

Query: 421 LADDFHLDSIRDRAKMSALLLYRLLQKGYSSIMTAIMDEEAAEKQRKRAAAAAALQSAPA 480
           + D+  L S+++RA+MS LLLYRL+QKGY ++++ I   EA                A A
Sbjct: 436 IIDEQELVSVKNRARMSTLLLYRLIQKGYYAVLSDIETVEA--------------DKAVA 495

Query: 481 IQLQHQEDGVDEMESKSVEISSHNNNNSKVVYQGVFCRDLAVVGNLLSQVGFGRPIAV 539
           I  +  E  +D  ++ ++ +   +N+   VV +GVFCRD+A VG+L+S+ G  +PI V
Sbjct: 496 IDNRRIETPMDGNKAVAMTVVRKSNDKLDVVVKGVFCRDMAAVGSLISRAGLRQPITV 513

BLAST of MS019003 vs. TAIR 10
Match: AT5G12430.1 (Heat shock protein DnaJ with tetratricopeptide repeat )

HSP 1 Score: 98.6 bits (244), Expect = 1.6e-20
Identity = 82/333 (24.62%), Postives = 150/333 (45.05%), Query Frame = 0

Query: 148  WRYIVLGQACCHLGLLEDAMVLLQTGKRLATAALRRESTFRSDDRFPLPNFPILFTSGHP 207
            W+  ++ ++  ++G LE+A+  L+  ++L +A  +RE     +   P             
Sbjct: 826  WQCHLMLKSSFYMGKLEEAIASLEKQEQLLSAT-KREGNKTLESSIP------------- 885

Query: 208  HPLTESETISSLLTHVKLLLRRRTAALAALDAGLHSEAIRHFTKILDGRRPAPQTFLAEC 267
                       L   ++ LLR + A   A  +G H+EA+ H+T  L       + F A C
Sbjct: 886  -----------LAATIRELLRLKAAGNEAFQSGRHTEAVEHYTAAL-ACNVESRPFTAVC 945

Query: 268  FMHRSSAYRSAGRIADSIADCNWTLTLDPTCIQALETRAELFESIGCLPDCLHDLEHLKL 327
            F +R++AY++ G+ +D+IADC+  + LD    +A+  RA LFE I        D+E    
Sbjct: 946  FCNRAAAYKALGQFSDAIADCSLAIALDQNYSKAISRRATLFEMIRDYGQAASDMER--- 1005

Query: 328  LYNSILRDRKLPGPAWKRHNVRYREIPGKLCALTARIQKVKQRVASGENGNVDYYSLIGL 387
             Y +IL  +       K      R          ARI+  +    S +  ++D Y ++G+
Sbjct: 1006 -YVNILTKQM----EEKTSGTLDRSTSMSNDIRQARIRLSELEEKSRKENSLDMYLVLGV 1065

Query: 388  RRGCSRSELQRAHLLLCLKHTPDKATGFLDRCELADDFHLDSIRDRAKMSALLLYRLLQK 447
               CS S++++A+    LKH PDKA   L R E  D+     I +  +     L++++ +
Sbjct: 1066 VPSCSASDIRKAYRKAALKHHPDKAGQSLTRNETKDERLWKEIGEEVRKDTDKLFKMIGE 1124

Query: 448  GYSSIMTAI------MDEEAAEKQRKRAAAAAA 475
             Y+ +          ++EE    Q++R  ++ +
Sbjct: 1126 AYAVLSDPAKRSQYDLEEEMHNSQKRRDGSSTS 1124

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906072.19.7e-23380.51uncharacterized protein LOC120091962 [Benincasa hispida][more]
XP_008454300.11.7e-23280.59PREDICTED: uncharacterized protein LOC103494740 [Cucumis melo][more]
KAA0044409.18.2e-23280.55Heat shock protein DnaJ with tetratricopeptide repeat isoform 1 [Cucumis melo va... [more]
XP_022998609.11.8e-23179.10uncharacterized protein LOC111493197 [Cucurbita maxima][more]
KAG7036648.12.4e-23179.20hypothetical protein SDJN02_00268 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3BXS68.0e-23380.59uncharacterized protein LOC103494740 OS=Cucumis melo OX=3656 GN=LOC103494740 PE=... [more]
A0A5D3E0U94.0e-23280.55Heat shock protein DnaJ with tetratricopeptide repeat isoform 1 OS=Cucumis melo ... [more]
A0A6J1KES58.9e-23279.10uncharacterized protein LOC111493197 OS=Cucurbita maxima OX=3661 GN=LOC111493197... [more]
A0A6J1G8X55.8e-23179.02uncharacterized protein LOC111451999 OS=Cucurbita moschata OX=3662 GN=LOC1114519... [more]
A0A0A0KT921.9e-22979.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G003100 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02100.11.7e-17961.54Heat shock protein DnaJ with tetratricopeptide repeat [more]
AT2G47440.12.3e-17660.95Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G62570.13.2e-16557.14Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02650.15.1e-14753.35Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G12430.11.6e-2024.62Heat shock protein DnaJ with tetratricopeptide repeat [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 213..338
e-value: 1.9E-10
score: 42.5
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 232..327
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 362..504
e-value: 2.4E-5
score: 26.2
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 376..414
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..113
NoneNo IPR availablePANTHERPTHR46816:SF1TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEINcoord: 1..545
NoneNo IPR availablePANTHERPTHR46816FAMILY NOT NAMEDcoord: 1..545
IPR001623DnaJ domainCDDcd06257DnaJcoord: 380..414
e-value: 6.77562E-7
score: 44.4585

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS019003.1MS019003.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding