MS018992 (gene) Bitter gourd (TR) v1

Overview


Name	MS018992
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold20: 519393 .. 519662 (-)
RNA-Seq Expression	MS018992
Synteny	MS018992

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGAACCCCCACCAATGCATATCTGGACCCTAAATGCCGATGCATCGTGGTCGGATTCTACGCATAGAGGAGGGATTGGGTGGATTATTCGCAGTTGGGACGACGACATTGTACTTGCGGGCAATCGTTTTGTGGAGGCATGCAATAATGTCAAACTTCTAGAGGCTTCGGCCATTCTTGAAGGTCTTCGCAGCTTGACAAATCTTGGAGTTCTTCACCCTCTTCATATTGAGACAGATTCTGCCGAAGTGGAAAGTTTGCTGAATAGG

mRNA sequence

TGGGAACCCCCACCAATGCATATCTGGACCCTAAATGCCGATGCATCGTGGTCGGATTCTACGCATAGAGGAGGGATTGGGTGGATTATTCGCAGTTGGGACGACGACATTGTACTTGCGGGCAATCGTTTTGTGGAGGCATGCAATAATGTCAAACTTCTAGAGGCTTCGGCCATTCTTGAAGGTCTTCGCAGCTTGACAAATCTTGGAGTTCTTCACCCTCTTCATATTGAGACAGATTCTGCCGAAGTGGAAAGTTTGCTGAATAGG

Coding sequence (CDS)

TGGGAACCCCCACCAATGCATATCTGGACCCTAAATGCCGATGCATCGTGGTCGGATTCTACGCATAGAGGAGGGATTGGGTGGATTATTCGCAGTTGGGACGACGACATTGTACTTGCGGGCAATCGTTTTGTGGAGGCATGCAATAATGTCAAACTTCTAGAGGCTTCGGCCATTCTTGAAGGTCTTCGCAGCTTGACAAATCTTGGAGTTCTTCACCCTCTTCATATTGAGACAGATTCTGCCGAAGTGGAAAGTTTGCTGAATAGG

Protein sequence

WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAILEGLRSLTNLGVLHPLHIETDSAEVESLLNR

Homology

[Close]

BLAST of MS018992 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 186.4 bits (472), Expect = 1.1e-43
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWD DIVLAGNRFVEACNNVKLLEASAIL
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLNR 91
           EGLR+LTNLGVL PLHIETDSAEVESLLNR
Sbjct: 131 EGLRNLTNLGVLRPLHIETDSAEVESLLNR 160

[Close]

BLAST of MS018992 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 76.6 bits (187), Expect = 1.2e-10
Identity = 36/90 (40.00%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W  PP + W LN DASWS+    GGIGWI+     +IVLAGN  +     +  LE   I+
Sbjct: 105 WSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTII 164

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLNR 91
            GL+ + N+    P+++E+DS EV  L+ +
Sbjct: 165 RGLQFI-NMQSRSPIYLESDSVEVIRLMKK 193

[Close]

BLAST of MS018992 vs. NCBI nr
Match: XP_022148549.1 (uncharacterized protein LOC111017181 [Momordica charantia])

HSP 1 Score: 62.4 bits (150), Expect = 2.3e-06
Identity = 31/90 (34.44%), Postives = 47/90 (52.22%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W PP  H+W LN DA+W DS H GG+GWI+R  +   ++A          +K L  S  L
Sbjct: 78  WTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDSEGRFIMA--------ECLKALSQSLTL 137

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLNR 91
           E        G+   + +E+D  EV +++N+
Sbjct: 138 EA-------GI--KIEMESDCLEVVNIINK 150

[Close]

BLAST of MS018992 vs. NCBI nr
Match: XP_038886170.1 (uncharacterized protein LOC120076417 [Benincasa hispida])

HSP 1 Score: 61.2 bits (147), Expect = 5.2e-06
Identity = 33/90 (36.67%), Postives = 49/90 (54.44%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W P     W LN DASW+      G+GW+       + +AG +FV  C  V +LEA AI 
Sbjct: 69  WHPSKPFYWKLNMDASWNSKIDACGLGWVFLDHLYRVCMAGLKFVLRCQKVNILEAIAIC 128

Query: 61  EGLRSLTNLGVLHPLHIETDS-AEVESLLN 90
            GL  L+++ + + + +E+D   EV +LLN
Sbjct: 129 FGLEILSSIDISN-IMVESDCLEEVINLLN 157

[Close]

BLAST of MS018992 vs. NCBI nr
Match: VDD57155.1 (unnamed protein product, partial [Brassica oleracea])

HSP 1 Score: 60.8 bits (146), Expect = 6.7e-06
Identity = 29/89 (32.58%), Postives = 48/89 (53.93%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W PPP +    N+D++W +     G+GWI R+   +++ AG R V    +  L EA A+ 
Sbjct: 112 WRPPPSNWVKCNSDSAWHNERDTSGLGWICRNETGEVLWAGARAVTKTGSAILAEAEALK 171

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLN 90
            G  +L N G  + +  ETDS ++  ++N
Sbjct: 172 WGAETLANFGYKN-IIFETDSLQLAKMIN 199

[Close]

BLAST of MS018992 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.2e-44
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWD DIVLAGNRFVEACNNVKLLEASAIL
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLNR 91
           EGLR+LTNLGVL PLHIETDSAEVESLLNR
Sbjct: 131 EGLRNLTNLGVLRPLHIETDSAEVESLLNR 160

[Close]

BLAST of MS018992 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 5.8e-11
Identity = 36/90 (40.00%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W  PP + W LN DASWS+    GGIGWI+     +IVLAGN  +     +  LE   I+
Sbjct: 105 WSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTII 164

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLNR 91
            GL+ + N+    P+++E+DS EV  L+ +
Sbjct: 165 RGLQFI-NMQSRSPIYLESDSVEVIRLMKK 193

[Close]

BLAST of MS018992 vs. ExPASy TrEMBL
Match: A0A5B6YN15 (RNase H domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_002651 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 8.6e-07
Identity = 28/84 (33.33%), Postives = 43/84 (51.19%), Query Frame = 0


Query: 1  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
          W PPP  ++ LN DASW      GG+G +IR W  D++    + ++ C++    E    L
Sbjct: 11 WSPPPEGLFKLNVDASWIPGEIIGGVGGVIRDWKGDVIAGFAKCIDQCSSAGYAELMGFL 70

Query: 61 EGLRSLTNLGVLHPLHIETDSAEV 85
           GL     +G+ H L +E D  +V
Sbjct: 71 HGLIFAKEIGI-HDLAVEGDCLKV 93

[Close]

BLAST of MS018992 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-06
Identity = 31/90 (34.44%), Postives = 47/90 (52.22%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W PP  H+W LN DA+W DS H GG+GWI+R  +   ++A          +K L  S  L
Sbjct: 78  WTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDSEGRFIMA--------ECLKALSQSLTL 137

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLNR 91
           E        G+   + +E+D  EV +++N+
Sbjct: 138 EA-------GI--KIEMESDCLEVVNIINK 150

[Close]

BLAST of MS018992 vs. ExPASy TrEMBL
Match: A0A3P6GJZ4 (RNase H domain-containing protein (Fragment) OS=Brassica oleracea OX=3712 GN=BOLC8T50384H PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.3e-06
Identity = 29/89 (32.58%), Postives = 48/89 (53.93%), Query Frame = 0


Query: 1   WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDDDIVLAGNRFVEACNNVKLLEASAIL 60
           W PPP +    N+D++W +     G+GWI R+   +++ AG R V    +  L EA A+ 
Sbjct: 112 WRPPPSNWVKCNSDSAWHNERDTSGLGWICRNETGEVLWAGARAVTKTGSAILAEAEALK 171

Query: 61  EGLRSLTNLGVLHPLHIETDSAEVESLLN 90
            G  +L N G  + +  ETDS ++  ++N
Sbjct: 172 WGAETLANFGYKN-IIFETDSLQLAKMIN 199

The following BLAST results are available for this feature:

BLAST of MS018992 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	1.1e-43	96.67	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143317.1	1.2e-10	40.00	uncharacterized protein LOC111013216 [Momordica charantia]	[more]
XP_022148549.1	2.3e-06	34.44	uncharacterized protein LOC111017181 [Momordica charantia]	[more]
XP_038886170.1	5.2e-06	36.67	uncharacterized protein LOC120076417 [Benincasa hispida]	[more]
VDD57155.1	6.7e-06	32.58	unnamed protein product, partial [Brassica oleracea]	[more]

BLAST of MS018992 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS018992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	5.2e-44	96.67	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CQG0	5.8e-11	40.00	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A5B6YN15	8.6e-07	33.33	RNase H domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 GN=...	[more]
A0A6J1D4B6	1.1e-06	34.44	uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A3P6GJZ4	3.3e-06	32.58	RNase H domain-containing protein (Fragment) OS=Brassica oleracea OX=3712 GN=BOL...	[more]

BLAST of MS018992 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 8..90 e-value: 1.7E-9 score: 39.8
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 12..89 e-value: 1.5E-14 score: 53.9
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 11..90 e-value: 3.61025E-10 score: 50.3904
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 6..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018992.1	MS018992.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity