MS018973 (gene) Bitter gourd (TR) v1

Overview


Name	MS018973
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold20: 398344 .. 402462 (+)
RNA-Seq Expression	MS018973
Synteny	MS018973

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATTATTCCAAATGGAGATTGATTTGGATAATACCAACGTTGTTGTTGTTTGTTTGTGTGGATGTTTCAAAAGCATTGGATTGTCAGCTGAATTTTAGGAATCCATATAGAGTTGCATACAGTATTTATGTTAGCAAAAAGGGGAGAGCAAACTTCAGAACAGTCCAAAGTGCCATTGATTCAATCCCTTTTAATAGTACCCAATGGATTAGGGTTCAAATTGGTCCTGGCATATATTGGTAAAAACTATTCACTTATCCTTCTATGTTTTTCTTTCTTTTTTTTCTTTTTGTTTTTTTTTTTTCTTACTTCTCGAAATTGATGTTGAGTGGCTTACACTGTGAAGCCCTACAACCGAGACTTATTATATGGGCTGAGCTTAAATCTATAACCTCATGGTGGGTGATACCACAAGTCCAAAGTCCTTGACACTACGTATCGCTCCAAAGCATTTACCCTTCTAGGTTTTGTAGTTAATTATTCCATTAATATGAGTTTTTTTTTTCTTTTTGGGTTCATTTTTAATGTTGTAATTTTTTGTAGTTAATTATTCCATTAATATGAGTTTTTTTTTTTTTTTTGGGTTCATTTTTAATGTTATAATTTTTTTTAGTTCAACAAACCTCCGGGATGAGACATTTTAGATGAGGGTATATATTTCAATTAGTTGAGCTAGACTTATATTGAATTGTTGATCATTTTAGTGTTGATACTTAATTTAGTGAGTTTATTTTAAATTTCAACTTTCCGTTTTAGGGGGTTGGACTCTAATTTTTTAGAATAAGTAATTAAATTAATATTTAAATCTCATTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGTGGAGTTGTTTTCGACCATCAATTATTTAACAAATGATATGAATATATATGACTTCATAAATGTGTATTAATTGCTTATTAATGTGAGTAGTACTATTTGAAGCTAACATGAACATAGTTCAATAGTTAAGGCACCTTCCTCCTTCCCTAAAAGTTAGAAGTTCAATCTCGCACCAACATATTTATTGTACTAAAAAGAAGTATATCGAAGCCACATTCACTAGCTAAGATGGATACTGGGGTGGGTAAATAAATATTGATAAAGATCTAGCCTTAAGAAGCTATTTTTTATTTAAGTAGAAACTTTTCCCCCTTGTTTTTTATAATAGAATAGGTAATTTATTTTAACATATAGATGGATTGTTTGAATTTTGGAAACAGGGAAAAGGTGGTAATTCCCGCAGGAAAAACATGCATATTTCTAGATGGGGCTGACAATAGATTCACTGAAATTCAATGGAATGACCATCAAGCCACAGCTACTAGTGCCACCTTCACTTCCTTTGCAGAAAATCTTCTAGTCAAAGGCATCACATTCAAGGTACTATTATTAATTACTTCTAAACTTTCGTGTTTCATTTCGTGTTTTAGGACATATTTAGGAGTGATTTTGGTATAGTCAAAATCACTCACTTTGTTCATATCTGACAAATTAAAATTTTGATTTTAGGAAAGTTTAAAATTATTTTAAACCAATTATTCATAAATCTTTTTTTTCAAAATCACTCATTATACCATTACTCTCAAACACACAAACTTTACATAAAAATATACTTTAATTCTTTCGATCCAAAACATAGCTCAAAAAATAAGGCACCTTATTACCTATCAGTTGTTTATTAGTGCAAATTTATATACAAAATTGTATCGACTTTACTATAGATAGATTGTGTCGATAAAGTCTCTAAACTTTTAAAATAACTAAATAGATACAACATTGAAAGATCGTATCAAAAACTAAACTTGTATTTGAACCACATATGAACCGAAACACATATGTCAATCGAAAGTTTATGGACCAAAATAGGATTTAAAACTTACGGAAAGTTATTGCGAATGGAAATGGGCAGAATACTTATAATACACCAGGAAGTGTGAGGAGACGAGAAGATATAGAACCAGCACTTGCAGTTCTTATAGAAGGAGACAAAGCAAGTTTCTATGAGTGTAGATTTATTGGACTGCAGGATACTCTGTGGGATGGAACTGGCCGTCATCATTTCGTCAATTGCTACATTGAAGGTGTAATCGATATCATTTCTGGTTCTGGCCAATCCATCTACGAGGTTAACTTTAAAATAAGTTCTAAATGTTATATTATTCGACTTATATTCAAGTTGGTTCTTTTTTTCGAGTTTAGATAGGGGTTTCAGAAAACCAAACTGTTTGAAATATATATGATAATTTATTGTTCCTCAGTTTTTAAGATAAAAAGATCTGTTTGGTAATTTTTTTAGTTTTAATAAAGAATAATGAATTACACAGTCAAACATCCATGAATTGATAAAACAATTTGTTTATTTATGTTTTAGAATCGATTGAAATTAGAAACTTGTTGCTTGTTTCTAAAATTTATATACAATTTTGGAGATGTTCCCAAAAAAATAAGAAACAAAAAACATACTTATTGCAAAATGATTAGATTTCTTAAAAAAATTGGAAACGATAAACAAGAAACGAAAACGTTACCTAGACCTAAAATTTCTTGTTTCTCATTTTCTGAAAAAGTTTCCAAAATTGTGTAAAAATTTAATTTGAAAAAAAAAATTAAATTTATTGTTTCTCCAACCAGTTTCCAAATTTTTTCAAAATATAAAGAAAAATATTATTTTATCATTCTAATCAATTTATGAATGAATAATAAATATTAAAAGTAAATATTTTGAATATTTAATTTAAAAAAACATTTTTATATAAAACTTAAAAAAACTTTTTAATTGGAACTAGAAATGGTTACCAAAAGCTGATATGTTTCAATTTAAATAAAATAAAAGAATAAGAAACAAGTAATAGTTACCAACAAAAATTTGTTTTTCTTTATTACATCACAAGAAACCAAAAAAATGAGAAACTACCGAACGGGCATCTAGTAAGTTATTTCGTCGGTTTTTTCATAGTAGCATGTCAGTTTCGTTACGTCGATTTTTATATTTTCCTTAGAGATTGGTGTTGATTTGAAGTTTTGATTTTGTATGTGTGTTTGTTTTTATGGCAGAAATGTAAGATAAATGTACCAATCGATCAATATTCTCCAATATTGAGTTACGGATACATAACAGCACAAGGAAAAGAGAATCCAAATGAAGGCAGTGGCTTTGTTTTCAATGCATGCTCAGTTGTTGGAAATGGGAGAGTTTATCTTGGAAGGGCTTATAGGCCTTTCTCCACTGTCATTTTCCATAATTCTTTTCTAGCAGGTTTCATTGACCCTGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGTAAGTTTACGTCCAGCATTATGTTTATTTAGTCATCGAACTTGAAACTTTTCAATAATATCTCTTAACTTAAGACATTATGTTTCTATATATATATATAGTCTCTATGGTTGTCTTCACGCATGACAAACAACTAAGTGATATTCTTAAATTTAGAATGTGTAATCGAAGCTTTTTGAAACTCGCACTAAGTATAGAGATTAAATTTATAGTTTAACTTTTATGGTTTTTCTTTCTATCATGTTTGTTAATTCGAGTCTAAAACTTAGAAGTCAACTAACTTCATGAAATATTGTCCACAATCAATAGCTTGATTGGCTAGGAGGTTCAAGAAATATCCTTGAGATGTTGCGTTCAAGTCACATAGAACGCAATGATCCCTTGCATATATACAGGTACAAGAACAATATGCATACCCCAGGTATAGGGGTAAAAATGCCAAGGGGCCACCTACTCGAGTAATAAAAACTTTATGAAATTAGACTCCTCAGTCATGCTTTAACCATTGGATAGTGATCAAGTATTTGTATTTTATTCTTCCAACAATAGTTTTGTCCAAGATCTCAAAATAACAACGTTATCAAATAATCTCTAAATTATTTTGTTCATGTATGGTCTTGTAGGAGGGATTTGACATATGCAGAGGCGAATTGTATTGGACCAGGAGCAGACACTTCAAGACGAGTCCCTTGGCTTAAGAGACTCAGTGGTGCCGAGCTCAAATACTTCTCACATATTTCGTTTATTGATCAAGATGGTTGGACTTCAAGACTTCCAATATTTGCT

mRNA sequence

ATGGAACATTATTCCAAATGGAGATTGATTTGGATAATACCAACGTTGTTGTTGTTTGTTTGTGTGGATGTTTCAAAAGCATTGGATTGTCAGCTGAATTTTAGGAATCCATATAGAGTTGCATACAGTATTTATGTTAGCAAAAAGGGGAGAGCAAACTTCAGAACAGTCCAAAGTGCCATTGATTCAATCCCTTTTAATAGTACCCAATGGATTAGGGTTCAAATTGGTCCTGGCATATATTGGGAAAAGGTGGTAATTCCCGCAGGAAAAACATGCATATTTCTAGATGGGGCTGACAATAGATTCACTGAAATTCAATGGAATGACCATCAAGCCACAGCTACTAGTGCCACCTTCACTTCCTTTGCAGAAAATCTTCTAGTCAAAGGCATCACATTCAAGAATACTTATAATACACCAGGAAGTGTGAGGAGACGAGAAGATATAGAACCAGCACTTGCAGTTCTTATAGAAGGAGACAAAGCAAGTTTCTATGAGTGTAGATTTATTGGACTGCAGGATACTCTGTGGGATGGAACTGGCCGTCATCATTTCGTCAATTGCTACATTGAAGGTGTAATCGATATCATTTCTGGTTCTGGCCAATCCATCTACGAGAAATGTAAGATAAATGTACCAATCGATCAATATTCTCCAATATTGAGTTACGGATACATAACAGCACAAGGAAAAGAGAATCCAAATGAAGGCAGTGGCTTTGTTTTCAATGCATGCTCAGTTGTTGGAAATGGGAGAGTTTATCTTGGAAGGGCTTATAGGCCTTTCTCCACTGTCATTTTCCATAATTCTTTTCTAGCAGGTTTCATTGACCCTGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGAGGGATTTGACATATGCAGAGGCGAATTGTATTGGACCAGGAGCAGACACTTCAAGACGAGTCCCTTGGCTTAAGAGACTCAGTGGTGCCGAGCTCAAATACTTCTCACATATTTCGTTTATTGATCAAGATGGTTGGACTTCAAGACTTCCAATATTTGCT

Coding sequence (CDS)

ATGGAACATTATTCCAAATGGAGATTGATTTGGATAATACCAACGTTGTTGTTGTTTGTTTGTGTGGATGTTTCAAAAGCATTGGATTGTCAGCTGAATTTTAGGAATCCATATAGAGTTGCATACAGTATTTATGTTAGCAAAAAGGGGAGAGCAAACTTCAGAACAGTCCAAAGTGCCATTGATTCAATCCCTTTTAATAGTACCCAATGGATTAGGGTTCAAATTGGTCCTGGCATATATTGGGAAAAGGTGGTAATTCCCGCAGGAAAAACATGCATATTTCTAGATGGGGCTGACAATAGATTCACTGAAATTCAATGGAATGACCATCAAGCCACAGCTACTAGTGCCACCTTCACTTCCTTTGCAGAAAATCTTCTAGTCAAAGGCATCACATTCAAGAATACTTATAATACACCAGGAAGTGTGAGGAGACGAGAAGATATAGAACCAGCACTTGCAGTTCTTATAGAAGGAGACAAAGCAAGTTTCTATGAGTGTAGATTTATTGGACTGCAGGATACTCTGTGGGATGGAACTGGCCGTCATCATTTCGTCAATTGCTACATTGAAGGTGTAATCGATATCATTTCTGGTTCTGGCCAATCCATCTACGAGAAATGTAAGATAAATGTACCAATCGATCAATATTCTCCAATATTGAGTTACGGATACATAACAGCACAAGGAAAAGAGAATCCAAATGAAGGCAGTGGCTTTGTTTTCAATGCATGCTCAGTTGTTGGAAATGGGAGAGTTTATCTTGGAAGGGCTTATAGGCCTTTCTCCACTGTCATTTTCCATAATTCTTTTCTAGCAGGTTTCATTGACCCTGCTGGTTGGGATCCTTGGGAACAAGTTGGCCATGAGAGGGATTTGACATATGCAGAGGCGAATTGTATTGGACCAGGAGCAGACACTTCAAGACGAGTCCCTTGGCTTAAGAGACTCAGTGGTGCCGAGCTCAAATACTTCTCACATATTTCGTTTATTGATCAAGATGGTTGGACTTCAAGACTTCCAATATTTGCT

Protein sequence

MEHYSKWRLIWIIPTLLLFVCVDVSKALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWTSRLPIFA

Homology

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BLAST of MS018973 vs. NCBI nr
Match: XP_022157021.1 (uncharacterized protein LOC111023848 [Momordica charantia])

HSP 1 Score: 679.5 bits (1752), Expect = 1.5e-191
Identity = 318/320 (99.38%), Postives = 319/320 (99.69%), Query Frame = 0


Query: 26  KALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKV 85
           +ALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKV
Sbjct: 334 EALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKV 393

Query: 86  VIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVR 145
           VIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVR
Sbjct: 394 VIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVR 453

Query: 146 RREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSI 205
           RREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSI
Sbjct: 454 RREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSI 513

Query: 206 YEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFST 265
           YEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFST
Sbjct: 514 YEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFST 573

Query: 266 VIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKY 325
           VIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLS AELKY
Sbjct: 574 VIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSAAELKY 633

Query: 326 FSHISFIDQDGWTSRLPIFA 346
           FSHISFIDQDGWTSRLPIFA
Sbjct: 634 FSHISFIDQDGWTSRLPIFA 653

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BLAST of MS018973 vs. NCBI nr
Match: XP_023524602.1 (LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 507.7 bits (1306), Expect = 8.0e-140
Identity = 227/335 (67.76%), Postives = 280/335 (83.58%), Query Frame = 0


Query: 10  IWIIPTLLLFVCVDVSKALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNST 69
           I +I TLLL +C++VSKALDC+LN  +P RVAY+I V K G  NFRT+QSAIDSIP  ++
Sbjct: 4   ILLITTLLLLLCLNVSKALDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNS 63

Query: 70  QWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLV 129
           +WIR+QI  GIY EKV I   K+CIFL+GA    TEI+WNDH  TATSATFTS AENL+V
Sbjct: 64  RWIRIQISSGIYSEKVHISEEKSCIFLEGAGRSSTEIRWNDHATTATSATFTSIAENLVV 123

Query: 130 KGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNC 189
           KGITFKNTYN PGSV+R+EDI PA+A LIEGDK  F++C F+GLQDTLWDG GRH +  C
Sbjct: 124 KGITFKNTYNVPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTEC 183

Query: 190 YIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVV 249
           YIEGVID+ISG+GQSIYE C+IN+P+D+Y+P++ +GYITAQGKE+ ++ +GFVF  CSV+
Sbjct: 184 YIEGVIDVISGAGQSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVI 243

Query: 250 GNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTS 309
           G+G VYLGRAYRPFSTVI+++SFL+  I+PAGWDPWEQVGHE  LTY E  CIGPGADTS
Sbjct: 244 GSGTVYLGRAYRPFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTS 303

Query: 310 RRVPWLKRLSGAELKYFSHISFIDQDGWTSRLPIF 345
           +RVPWLK+LS  E++ F+ ISF+DQ+GWTS+LP+F
Sbjct: 304 KRVPWLKKLSIDEIRRFTSISFVDQEGWTSKLPLF 338

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BLAST of MS018973 vs. NCBI nr
Match: KAG6606930.1 (putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 503.1 bits (1294), Expect = 2.0e-138
Identity = 227/335 (67.76%), Postives = 279/335 (83.28%), Query Frame = 0


Query: 10  IWIIPTLLLFVCVDVSKALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNST 69
           I +I TLL+ +C++VSKALDC+LN  +P RVAY+I V K G  NFRT+QSAIDSIP  ++
Sbjct: 4   ISLITTLLMLLCLNVSKALDCELNPNDPNRVAYTIVVDKSGSGNFRTIQSAIDSIPSQNS 63

Query: 70  QWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLV 129
           +WIR+QI  GIY EKV I   K+CIFLDGA    TEI+WNDH  TATSATFTS AENL+V
Sbjct: 64  RWIRIQISSGIYLEKVHISEEKSCIFLDGAGRSSTEIRWNDHATTATSATFTSIAENLVV 123

Query: 130 KGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNC 189
           KGITFKNTYN PGSV+R+EDI PA+A LIEGDK  F++C F+GLQDTLWDG GRH +  C
Sbjct: 124 KGITFKNTYNAPGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTEC 183

Query: 190 YIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVV 249
           YIEGVID+ISG+GQSIYE C+INVP+D Y+P++ +GYITAQGKE+ ++ +GFVF  CSV+
Sbjct: 184 YIEGVIDVISGAGQSIYENCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVI 243

Query: 250 GNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTS 309
           G+G VYLGRAYRPFSTVI+++SFL+  IDPAGWDPWEQVGHE  LT AE +C+G GADTS
Sbjct: 244 GSGTVYLGRAYRPFSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTCAETHCVGLGADTS 303

Query: 310 RRVPWLKRLSGAELKYFSHISFIDQDGWTSRLPIF 345
           +RV WLK+LS  E+++F+ ISFIDQ+GWTS+LP+F
Sbjct: 304 KRVSWLKKLSIDEIRHFTSISFIDQEGWTSKLPLF 338

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BLAST of MS018973 vs. NCBI nr
Match: KAE8650757.1 (hypothetical protein Csa_017626 [Cucumis sativus])

HSP 1 Score: 467.6 bits (1202), Expect = 9.2e-128
Identity = 216/335 (64.48%), Postives = 266/335 (79.40%), Query Frame = 0


Query: 1   MEH-YSKWRLIWIIPTLLLFVCVDVSKAL-DCQLNFRNPYRVAYSIYVSKKGRANFRTVQ 60
           MEH YSK   ++II TL++  CV VSKAL DC+LN+ +P++VAY+I V K G+ NF T+Q
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSA 120
           SAI+S+P  ST+WIR+QI PGIY EKV IP+ K+CIFLDG+  + TEI WNDH+ TA S 
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLW 180
           TFT+ A+NL+V+GITF+NTYN  GSV RREDI+PALA LI+GDK  F++C FIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEG 240
           DG GRH F  CYIEGVID+ISG GQSIY++C IN+P++ Y+P+L+ G+ITAQGKENPNE 
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAE 300
           SGFVF  C V G+G V+LGRAYRPFSTVIFH  FL   I+PAGW+ W Q GHE DLTY+E
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 ANCIGPGADTSRRVPWLKRLSGAELKYFSHISFID 334
             CIGPGADTS RVPW+ RL    ++ F+ IS+ID
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYID 335

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BLAST of MS018973 vs. NCBI nr
Match: XP_016900403.1 (PREDICTED: probable pectinesterase 55 [Cucumis melo])

HSP 1 Score: 448.0 bits (1151), Expect = 7.5e-122
Identity = 218/363 (60.06%), Postives = 265/363 (73.00%), Query Frame = 0


Query: 1   MEHYSKWRLIWIIPTLLLFVCVDVSKALD-CQLNFRNPYRVAYSIYVSKKGRANFRTVQS 60
           MEHYSK   + I   LLLF  V+VSKALD C+LN+ +P++VAY+I V K GR NF T+QS
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60

Query: 61  AIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSAT 120
           AIDS+P  S +WIR+QI PGIY               DG+  R TEIQWNDH  TATS T
Sbjct: 61  AIDSVPSQSNRWIRIQISPGIY-------------TYDGSGFRVTEIQWNDHATTATSPT 120

Query: 121 FTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWD 180
           FTS+A+NL+V+GITF+NTYN  GSV RREDI+PA+A LI+GDKA F++C FIGLQDTLWD
Sbjct: 121 FTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWD 180

Query: 181 GTGRHHFVNCYIEGVIDIISGSGQSI----------------YEKCKINVPIDQYSPILS 240
           G GRH F  CYIEGVID+ISG G  I                 ++C IN+PI+ Y+P+L+
Sbjct: 181 GPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLN 240

Query: 241 YGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWD 300
            G+ITAQGKENP+E SGFVF  C V G+G+V+LGRAYRPFSTVIFH  FL   IDPAGW+
Sbjct: 241 EGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWN 300

Query: 301 PWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDG-WTSRLP 346
            W+QVGHE +LTY+E  CIGPGADTS RVPWLKRL   +++ F+ IS+ID  G WTSRLP
Sbjct: 301 SWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 350

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BLAST of MS018973 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-85
Identity = 145/304 (47.70%), Postives = 199/304 (65.46%), Query Frame = 0


Query: 42  YSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADN 101
           + + V + G  NF T+Q AIDS+P N+T W  + +  G+Y EK+ IP  K  I + GA  
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGK 86

Query: 102 RFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTP--GSVRRREDIEPALAVLIE 161
           R T ++W+DH + A S TF + A+N +VK ITF N+YN P  G + +   + PA+A  I 
Sbjct: 87  RSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV-PAVAAFIG 146

Query: 162 GDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYS 221
           GDK++FY   F G+QDTLWD  GRH+F  C I+G +D I GSGQSIY+ C I V   Q  
Sbjct: 147 GDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLG 206

Query: 222 PILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDP 281
           P ++ GYITAQG+ N N+ +GFVF  C V G G+ YLGRA+RP+S VIF+NS L   +DP
Sbjct: 207 PGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 266

Query: 282 AGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWTS 341
            GW  W   G+E+ LTYAE  C G G++TSRR  W+K+LS + +++ + +SFI++ GW  
Sbjct: 267 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 326

Query: 342 RLPI 344
            LPI
Sbjct: 327 DLPI 328

[Close]

BLAST of MS018973 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 5.0e-84
Identity = 153/305 (50.16%), Postives = 205/305 (67.21%), Query Frame = 0


Query: 39  RVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDG 98
           ++AY+I V   G  NF TVQSAIDSI   +  WIRV    GIY EKV IP  K  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 99  ADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLI 158
                T I+++DHQAT  SATFT+FA+++++ GITFKNTYN   + +R  +I PA+A  +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPNNKR--EIVPAVAARM 149

Query: 159 EGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQY 218
            GD+    +  F+GLQDTL+DG GRH++  C I G ID I G GQS++++C +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 219 SPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFID 278
           +P   YG ITA  + +P++  GFVF+ C+V G G+  LGRA+   + VIF  S L+  + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 279 PAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWT 338
           P GWD W   G+ERDLT+ EA C G GADTS+RVPWLK+LS +E+  F+ +SFIDQDGW 
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWI 329

Query: 339 SRLPI 344
           SR PI
Sbjct: 330 SRFPI 332

[Close]

BLAST of MS018973 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 9.3e-83
Identity = 141/301 (46.84%), Postives = 193/301 (64.12%), Query Frame = 0


Query: 44  IYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRF 103
           ++V + G  NF T+Q AIDS+P N+  W  + +  G+Y EK+ IP  K  I L GA  R 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 104 TEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIE-PALAVLIEGDK 163
           T ++W+DH + A S TF++ A+N +VK ITF N+YN P   +  ++   PA+A LI GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 164 ASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYSPIL 223
           ++FY   F G+QDTLWD  GRH+F  C I+G +D I G+GQSIY+ C I V   Q  P L
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGL 213

Query: 224 SYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGW 283
           + GYITAQG+ NP + +GF+F  C V G G  +LGR +R +S VIF+NS L   + P GW
Sbjct: 214 A-GYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 284 DPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWTSRLP 343
           D W  VGHE  L +AE  C G GA+  RRV W+K+LS + ++  + +SFI++ GW   LP
Sbjct: 274 DAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 333

[Close]

BLAST of MS018973 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 8.7e-81
Identity = 142/305 (46.56%), Postives = 195/305 (63.93%), Query Frame = 0


Query: 39  RVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDG 98
           ++A +I V+      F+TVQSAIDSIP  +  WIR+ I  GIY EKV IP GK  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 99  ADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLI 158
                T I + DHQ T TSATFTS+  N+++ GITFKN YN   S       +PA+A ++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 159 EGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQY 218
            GDK +  +  F G QDTL+D  GRH++  C I G ID I G  QSI+E C + + +  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 219 SPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFID 278
            P   YG ITAQG+++P +  GFVF  C+V+G+G+  LGRA++ +S VIF+ S  +  I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 279 PAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWT 338
           P GWD W+  G E  +T+ E  C G GADTS+RVPWL + S  ++  F++++FID++GW 
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWL 335

Query: 339 SRLPI 344
           SRLPI
Sbjct: 336 SRLPI 337

[Close]

BLAST of MS018973 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 7.7e-69
Identity = 125/252 (49.60%), Postives = 162/252 (64.29%), Query Frame = 0


Query: 98  GADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRRE----DIEPA 157
           G   R T I +N H AT  S+TFTS+  +++V+ ++  NTYN   S+ +      DI+PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 158 LAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINV 217
           +A+ + GDK++FY C F+GLQDT+WD  GRHHF NCYIEG ID I GSGQS+YE C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 218 PIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFL 277
                +  +S+GYITAQG+ + ++ SGFVF   SV G+  VYLGRAY PFS VIF  + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 278 AGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELK-YFSHISFI 337
           +  + P GW  W   G+E   TYAE  C G G+D SRRVPW+ +L     K  FS  +FI
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 338 DQDGWTSRLPIF 345
           DQD W S +P F
Sbjct: 282 DQDQWISNIPRF 293

[Close]

BLAST of MS018973 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.4e-192
Identity = 318/320 (99.38%), Postives = 319/320 (99.69%), Query Frame = 0


Query: 26  KALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKV 85
           +ALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKV
Sbjct: 334 EALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKV 393

Query: 86  VIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVR 145
           VIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVR
Sbjct: 394 VIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVR 453

Query: 146 RREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSI 205
           RREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSI
Sbjct: 454 RREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSI 513

Query: 206 YEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFST 265
           YEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFST
Sbjct: 514 YEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFST 573

Query: 266 VIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKY 325
           VIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLS AELKY
Sbjct: 574 VIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSAAELKY 633

Query: 326 FSHISFIDQDGWTSRLPIFA 346
           FSHISFIDQDGWTSRLPIFA
Sbjct: 634 FSHISFIDQDGWTSRLPIFA 653

[Close]

BLAST of MS018973 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.6e-132
Identity = 222/348 (63.79%), Postives = 275/348 (79.02%), Query Frame = 0


Query: 1   MEH-YSKWRLIWIIPTLLLFVCVDVSKAL-DCQLNFRNPYRVAYSIYVSKKGRANFRTVQ 60
           MEH YSK   ++II TL++  CV VSKAL DC+LN+ +P++VAY+I V K G+ NF T+Q
Sbjct: 1   MEHYYSKEISLFIITTLVVLFCVKVSKALDDCELNYNDPHKVAYTIVVDKLGKGNFTTIQ 60

Query: 61  SAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSA 120
           SAI+S+P  ST+WIR+QI PGIY EKV IP+ K+CIFLDG+  + TEI WNDH+ TA S 
Sbjct: 61  SAINSVPSQSTKWIRIQISPGIYTEKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASP 120

Query: 121 TFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLW 180
           TFT+ A+NL+V+GITF+NTYN  GSV RREDI+PALA LI+GDK  F++C FIGLQDTLW
Sbjct: 121 TFTASAQNLVVQGITFRNTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLW 180

Query: 181 DGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEG 240
           DG GRH F  CYIEGVID+ISG GQSIY++C IN+P++ Y+P+L+ G+ITAQGKENPNE 
Sbjct: 181 DGPGRHLFTQCYIEGVIDVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNES 240

Query: 241 SGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAE 300
           SGFVF  C V G+G V+LGRAYRPFSTVIFH  FL   I+PAGW+ W Q GHE DLTY+E
Sbjct: 241 SGFVFLRCIVQGSGNVFLGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSE 300

Query: 301 ANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDG-WTSRLPIFA 346
             CIGPGADTS RVPW+ RL    ++ F+ IS+ID  G WTSR+P+ +
Sbjct: 301 TRCIGPGADTSSRVPWVNRLDAFHIRSFTDISYIDPQGYWTSRIPLLS 348

[Close]

BLAST of MS018973 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.6e-122
Identity = 218/363 (60.06%), Postives = 265/363 (73.00%), Query Frame = 0


Query: 1   MEHYSKWRLIWIIPTLLLFVCVDVSKALD-CQLNFRNPYRVAYSIYVSKKGRANFRTVQS 60
           MEHYSK   + I   LLLF  V+VSKALD C+LN+ +P++VAY+I V K GR NF T+QS
Sbjct: 1   MEHYSKEITLLITTLLLLFCHVNVSKALDYCELNYNDPHKVAYTIVVDKFGRGNFHTIQS 60

Query: 61  AIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSAT 120
           AIDS+P  S +WIR+QI PGIY               DG+  R TEIQWNDH  TATS T
Sbjct: 61  AIDSVPSQSNRWIRIQISPGIY-------------TYDGSGFRVTEIQWNDHATTATSPT 120

Query: 121 FTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWD 180
           FTS+A+NL+V+GITF+NTYN  GSV RREDI+PA+A LI+GDKA F++C FIGLQDTLWD
Sbjct: 121 FTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWD 180

Query: 181 GTGRHHFVNCYIEGVIDIISGSGQSI----------------YEKCKINVPIDQYSPILS 240
           G GRH F  CYIEGVID+ISG G  I                 ++C IN+PI+ Y+P+L+
Sbjct: 181 GPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLN 240

Query: 241 YGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWD 300
            G+ITAQGKENP+E SGFVF  C V G+G+V+LGRAYRPFSTVIFH  FL   IDPAGW+
Sbjct: 241 EGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWN 300

Query: 301 PWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDG-WTSRLP 346
            W+QVGHE +LTY+E  CIGPGADTS RVPWLKRL   +++ F+ IS+ID  G WTSRLP
Sbjct: 301 SWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDISYIDPQGYWTSRLP 350

[Close]

BLAST of MS018973 vs. ExPASy TrEMBL
Match: A0A061DSC1 (Pectinesterase OS=Theobroma cacao OX=3641 GN=TCM_004838 PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 4.8e-106
Identity = 181/334 (54.19%), Postives = 241/334 (72.16%), Query Frame = 0


Query: 9   LIWIIPTLLLFVCVDVSKALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDSIPFNS 68
           LI+I+  LL F    +SKAL+CQLN  N ++VA++I V + G+ +F+T+Q+AIDSIP N+
Sbjct: 26  LIFIV--LLYF---SISKALECQLNNANKFKVAHTIRVDQSGKGDFKTIQNAIDSIPSNN 85

Query: 69  TQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLL 128
            +WIR+ I PG + EKV IPA K CIFL+GA ++ T I+W DH  T  SATFTS  +N++
Sbjct: 86  DKWIRILISPGHFREKVTIPANKPCIFLEGAGSKLTSIEWGDHMDTCDSATFTSSPDNIV 145

Query: 129 VKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVN 188
            KGI+FKN YN P     ++ +EPALAV I GDK++FY C F GLQDTLWD  GRH+F N
Sbjct: 146 AKGISFKNIYNIPSGPDAKKKVEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGRHYFYN 205

Query: 189 CYIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSV 248
           CYIEG +D I GSGQSIYEKC+IN+   +Y+P    GYITAQG+ + ++ SGFVF  C  
Sbjct: 206 CYIEGAVDFIFGSGQSIYEKCEINLTAGKYAPEYPNGYITAQGRNSSDDPSGFVFKYCVF 265

Query: 249 VGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADT 308
            G G+ YLGRAY  +S VI + S L+  I  +GWD W  V HE +L Y EA+C GPGA+T
Sbjct: 266 TGYGKTYLGRAYGAYSRVIIYKSVLSDAILASGWDAWRYVHHEGNLIYVEADCKGPGANT 325

Query: 309 SRRVPWLKRLSGAELKYFSHISFIDQDGWTSRLP 343
           S+RVPWLK+L  ++LK F ++S+ID++GW ++LP
Sbjct: 326 SKRVPWLKKLGASQLKKFLNLSYIDKEGWIAKLP 354

[Close]

BLAST of MS018973 vs. ExPASy TrEMBL
Match: A0A6J1A2L9 (Pectinesterase OS=Herrania umbratica OX=108875 GN=LOC110414390 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.4e-105
Identity = 179/339 (52.80%), Postives = 238/339 (70.21%), Query Frame = 0


Query: 6   KWRLIWIIPTLLLFVCV--DVSKALDCQLNFRNPYRVAYSIYVSKKGRANFRTVQSAIDS 65
           K R +  +  L+  V +   +SKAL+CQLN  N + VA++I V + G+ +F+T+Q+AIDS
Sbjct: 16  KMRFLQHVLALIFIVLLYFSISKALECQLNNANKFNVAHTIRVDQSGKGDFKTIQNAIDS 75

Query: 66  IPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSF 125
           IP N+ +WIR+ I PG + EKV IPA K CIFL GA  + T I+W DH  T  SATFTS 
Sbjct: 76  IPSNNDKWIRILISPGNFREKVTIPANKPCIFLRGAGTKLTSIEWGDHMDTCDSATFTSS 135

Query: 126 AENLLVKGITFKNTYNTPGSVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGR 185
            +N++ KGITFKN YN       ++ +EPALAV I GDK++FY C F GLQDTLWD  GR
Sbjct: 136 PDNIVAKGITFKNIYNIQPGHDAKKKMEPALAVRIYGDKSAFYNCAFFGLQDTLWDVQGR 195

Query: 186 HHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVF 245
           H+F NCYIEG +D I G GQSIYE+C+IN+   +Y+P    GYITAQG+ +  + SGFVF
Sbjct: 196 HYFYNCYIEGAVDFIFGGGQSIYERCEINLTAGKYAPAYPNGYITAQGRNSSADPSGFVF 255

Query: 246 NACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIG 305
             C   G+G+ YLGRAY  +S VI +NS L+  I P+GWD W  V HE +L Y EA+C G
Sbjct: 256 KYCVFTGSGKTYLGRAYGAYSRVIIYNSVLSDAILPSGWDAWHYVHHEENLIYVEADCKG 315

Query: 306 PGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWTSRLP 343
           PGA+TS+RVPWL++LS ++L  F +IS+ID++GW ++LP
Sbjct: 316 PGANTSKRVPWLRKLSASQLNQFLNISYIDKEGWIAKLP 354

[Close]

BLAST of MS018973 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 8.4e-87
Identity = 145/304 (47.70%), Postives = 199/304 (65.46%), Query Frame = 0


Query: 42  YSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADN 101
           + + V + G  NF T+Q AIDS+P N+T W  + +  G+Y EK+ IP  K  I + GA  
Sbjct: 27  FEVIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGK 86

Query: 102 RFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTP--GSVRRREDIEPALAVLIE 161
           R T ++W+DH + A S TF + A+N +VK ITF N+YN P  G + +   + PA+A  I 
Sbjct: 87  RSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRV-PAVAAFIG 146

Query: 162 GDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYS 221
           GDK++FY   F G+QDTLWD  GRH+F  C I+G +D I GSGQSIY+ C I V   Q  
Sbjct: 147 GDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLG 206

Query: 222 PILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDP 281
           P ++ GYITAQG+ N N+ +GFVF  C V G G+ YLGRA+RP+S VIF+NS L   +DP
Sbjct: 207 PGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 266

Query: 282 AGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWTS 341
            GW  W   G+E+ LTYAE  C G G++TSRR  W+K+LS + +++ + +SFI++ GW  
Sbjct: 267 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVE 326

Query: 342 RLPI 344
            LPI
Sbjct: 327 DLPI 328

[Close]

BLAST of MS018973 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 3.5e-85
Identity = 153/305 (50.16%), Postives = 205/305 (67.21%), Query Frame = 0


Query: 39  RVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDG 98
           ++AY+I V   G  NF TVQSAIDSI   +  WIRV    GIY EKV IP  K  I+L G
Sbjct: 30  QIAYTITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKGFIYLQG 89

Query: 99  ADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLI 158
                T I+++DHQAT  SATFT+FA+++++ GITFKNTYN   + +R  +I PA+A  +
Sbjct: 90  KGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPNNKR--EIVPAVAARM 149

Query: 159 EGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQY 218
            GD+    +  F+GLQDTL+DG GRH++  C I G ID I G GQS++++C +N+ +  Y
Sbjct: 150 LGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMTLGIY 209

Query: 219 SPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFID 278
           +P   YG ITA  + +P++  GFVF+ C+V G G+  LGRA+   + VIF  S L+  + 
Sbjct: 210 APDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLSDVVL 269

Query: 279 PAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWT 338
           P GWD W   G+ERDLT+ EA C G GADTS+RVPWLK+LS +E+  F+ +SFIDQDGW 
Sbjct: 270 PIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQDGWI 329

Query: 339 SRLPI 344
           SR PI
Sbjct: 330 SRFPI 332

[Close]

BLAST of MS018973 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 308.5 bits (789), Expect = 6.6e-84
Identity = 141/301 (46.84%), Postives = 193/301 (64.12%), Query Frame = 0


Query: 44  IYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDGADNRF 103
           ++V + G  NF T+Q AIDS+P N+  W  + +  G+Y EK+ IP  K  I L GA  R 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 104 TEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIE-PALAVLIEGDK 163
           T ++W+DH + A S TF++ A+N +VK ITF N+YN P   +  ++   PA+A LI GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 164 ASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQYSPIL 223
           ++FY   F G+QDTLWD  GRH+F  C I+G +D I G+GQSIY+ C I V   Q  P L
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGL 213

Query: 224 SYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFIDPAGW 283
           + GYITAQG+ NP + +GF+F  C V G G  +LGR +R +S VIF+NS L   + P GW
Sbjct: 214 A-GYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGW 273

Query: 284 DPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWTSRLP 343
           D W  VGHE  L +AE  C G GA+  RRV W+K+LS + ++  + +SFI++ GW   LP
Sbjct: 274 DAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 333

[Close]

BLAST of MS018973 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 6.2e-82
Identity = 142/305 (46.56%), Postives = 195/305 (63.93%), Query Frame = 0


Query: 39  RVAYSIYVSKKGRANFRTVQSAIDSIPFNSTQWIRVQIGPGIYWEKVVIPAGKTCIFLDG 98
           ++A +I V+      F+TVQSAIDSIP  +  WIR+ I  GIY EKV IP GK  I++ G
Sbjct: 36  QIAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQG 95

Query: 99  ADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRREDIEPALAVLI 158
                T I + DHQ T TSATFTS+  N+++ GITFKN YN   S       +PA+A ++
Sbjct: 96  GGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS---SSPTKPAVAAMM 155

Query: 159 EGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINVPIDQY 218
            GDK +  +  F G QDTL+D  GRH++  C I G ID I G  QSI+E C + + +  Y
Sbjct: 156 LGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIY 215

Query: 219 SPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFLAGFID 278
            P   YG ITAQG+++P +  GFVF  C+V+G+G+  LGRA++ +S VIF+ S  +  I 
Sbjct: 216 PPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNIL 275

Query: 279 PAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELKYFSHISFIDQDGWT 338
           P GWD W+  G E  +T+ E  C G GADTS+RVPWL + S  ++  F++++FID++GW 
Sbjct: 276 PIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWL 335

Query: 339 SRLPI 344
           SRLPI
Sbjct: 336 SRLPI 337

[Close]

BLAST of MS018973 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 5.5e-70
Identity = 125/252 (49.60%), Postives = 162/252 (64.29%), Query Frame = 0


Query: 98  GADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPGSVRRRE----DIEPA 157
           G   R T I +N H AT  S+TFTS+  +++V+ ++  NTYN   S+ +      DI+PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 158 LAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSGQSIYEKCKINV 217
           +A+ + GDK++FY C F+GLQDT+WD  GRHHF NCYIEG ID I GSGQS+YE C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 218 PIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRPFSTVIFHNSFL 277
                +  +S+GYITAQG+ + ++ SGFVF   SV G+  VYLGRAY PFS VIF  + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 278 AGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSGAELK-YFSHISFI 337
           +  + P GW  W   G+E   TYAE  C G G+D SRRVPW+ +L     K  FS  +FI
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 338 DQDGWTSRLPIF 345
           DQD W S +P F
Sbjct: 282 DQDQWISNIPRF 293

The following BLAST results are available for this feature:

BLAST of MS018973 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157021.1	1.5e-191	99.38	uncharacterized protein LOC111023848 [Momordica charantia]	[more]
XP_023524602.1	8.0e-140	67.76	LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo]	[more]
KAG6606930.1	2.0e-138	67.76	putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAE8650757.1	9.2e-128	64.48	hypothetical protein Csa_017626 [Cucumis sativus]	[more]
XP_016900403.1	7.5e-122	60.06	PREDICTED: probable pectinesterase 55 [Cucumis melo]	[more]

BLAST of MS018973 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E9D3	1.2e-85	47.70	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
Q4PSQ5	5.0e-84	50.16	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
Q4PSN0	9.3e-83	46.84	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
O64479	8.7e-81	46.56	Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1	[more]
O04953	7.7e-69	49.60	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]

BLAST of MS018973 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWQ8	7.4e-192	99.38	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1	[more]
A0A0A0LB12	6.6e-132	63.79	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1	[more]
A0A1S4DWQ1	3.6e-122	60.06	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1	[more]
A0A061DSC1	4.8e-106	54.19	Pectinesterase OS=Theobroma cacao OX=3641 GN=TCM_004838 PE=4 SV=1	[more]
A0A6J1A2L9	1.4e-105	52.80	Pectinesterase OS=Herrania umbratica OX=108875 GN=LOC110414390 PE=4 SV=1	[more]

BLAST of MS018973 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18990.1	8.4e-87	47.70	Pectin lyase-like superfamily protein	[more]
AT2G47280.1	3.5e-85	50.16	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	6.6e-84	46.84	Pectin lyase-like superfamily protein	[more]
AT2G19150.1	6.2e-82	46.56	Pectin lyase-like superfamily protein	[more]
AT5G26810.1	5.5e-70	49.60	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 38..343 e-value: 1.8E-90 score: 305.6
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 44..334 e-value: 2.5E-54 score: 184.1
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 12..343
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 12..343
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 40..340

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018973.1	MS018973.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity