MS018955 (gene) Bitter gourd (TR) v1

BLAST of MS018955 vs. NCBI nr
Match: XP_038905016.1 (GDSL esterase/lipase At1g09390-like [Benincasa hispida])

HSP 1 Score: 669.1 bits (1725), Expect = 2.2e-188
Identity = 314/373 (84.18%), Postives = 346/373 (92.76%), Query Frame = 0

Query: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEG 60
           MNLITEMA+  R SFPF L VIFFLLSLP PL VHSQ +QKPVIFNFGDSNSDTGG+SEG
Sbjct: 1   MNLITEMAKTKRNSFPFPLPVIFFLLSLPGPLFVHSQCTQKPVIFNFGDSNSDTGGFSEG 60

Query: 61  LGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120
           LGL+FG P GRTFFHKPSGRLCDGRLMIDFLCE+VSSDYLTPYLRSVG NFT+GANFAIS
Sbjct: 61  LGLKFGLPTGRTFFHKPSGRLCDGRLMIDFLCENVSSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180
           GS+TLP++ PF+L++Q++QFLQFQS SLELI KGYKDLVD EGF NALYTIDIGQNDLA 
Sbjct: 121 GSATLPKDRPFSLHIQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAA 180

Query: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASD 240
           +FTYLSYPQVIQ+IPSFVSEI++AIW+IY+HGG+ FWVHNTGPLGC+PQKLA T++NASD
Sbjct: 181 AFTYLSYPQVIQKIPSFVSEIKNAIWNIYEHGGKKFWVHNTGPLGCLPQKLAATSSNASD 240

Query: 241 IDNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFEN 300
           +DNHGCLQ+ NNAAKEFN QLKAACGELRSALTNATIVYVDVYA+KY+LIANS SNGFEN
Sbjct: 241 LDNHGCLQSQNNAAKEFNAQLKAACGELRSALTNATIVYVDVYAIKYDLIANSVSNGFEN 300

Query: 301 PLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEY 360
           PL+VCCGYGGPPYNFNQSITCGQ GFN C+EGLKY+SWDGVHYTEAANAVFASKILSS+Y
Sbjct: 301 PLIVCCGYGGPPYNFNQSITCGQPGFNTCNEGLKYVSWDGVHYTEAANAVFASKILSSQY 360

Query: 361 STPNFHFNFFCNK 374
           S P  HFNFFCNK
Sbjct: 361 SFPKLHFNFFCNK 373

BLAST of MS018955 vs. NCBI nr
Match: XP_004150835.1 (GDSL esterase/lipase At1g09390 [Cucumis sativus] >KGN58540.1 hypothetical protein Csa_001237 [Cucumis sativus])

HSP 1 Score: 659.8 bits (1701), Expect = 1.4e-185
Identity = 310/375 (82.67%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEG 60
           MNLITEM E+ R S PF LF+IFFLLSLPDPL VHSQ  QKPVIFNFGDSNSDTGG+SEG
Sbjct: 1   MNLITEMEEEKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60

Query: 61  LGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120
           LG++FG P GRTFFHKP+GRLCDGRLMIDFLCESV+SDYLTPYLRSVG NFT+GANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180
           GS+TLP++ PFNL +Q++QFLQFQS SLELI KGYKDLVD EGF NALYTIDIGQNDLA 
Sbjct: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAA 180

Query: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLAT--TAANA 240
           +FTYLSYPQVIQ+IPSFVSEI++AIW+IYQHGGRNFW+HNTGPLGC+PQKLAT    ANA
Sbjct: 181 AFTYLSYPQVIQQIPSFVSEIKNAIWTIYQHGGRNFWIHNTGPLGCLPQKLATAYADANA 240

Query: 241 SDIDNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGF 300
           SDIDNHGCLQ+ NNAAKEFNTQL+ ACGELRSALTNAT+VYVDVYA+KY+LI NS SNGF
Sbjct: 241 SDIDNHGCLQSFNNAAKEFNTQLRVACGELRSALTNATLVYVDVYAIKYDLITNSVSNGF 300

Query: 301 ENPLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSS 360
           ENPL+VCCGYGGPPYNFNQ++TCGQ GFN C+EGLKY+SWDGVHYTEAANAVFAS ILSS
Sbjct: 301 ENPLIVCCGYGGPPYNFNQTVTCGQPGFNTCNEGLKYVSWDGVHYTEAANAVFASMILSS 360

Query: 361 EYSTPNFHFNFFCNK 374
           +YS+P   FNFFCNK
Sbjct: 361 QYSSPKLPFNFFCNK 375

BLAST of MS018955 vs. NCBI nr
Match: XP_022998461.1 (GDSL esterase/lipase At1g09390-like [Cucurbita maxima])

HSP 1 Score: 655.2 bits (1689), Expect = 3.4e-184
Identity = 308/366 (84.15%), Postives = 333/366 (90.98%), Query Frame = 0

Query: 7   MAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFG 66
           M EK R SFPF LFVIFFLLSLP PL V SQ SQKPVIFNFGDSNSDTGG+SEGLGLRFG
Sbjct: 1   MTEKKRNSFPFPLFVIFFLLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFG 60

Query: 67  PPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAISGSSTLP 126
           PP+GR FFHKPSGRLCDGRLMIDFLCESV SDYLTPYLRSVGSNFT+G NFAISGS+TLP
Sbjct: 61  PPSGRVFFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLP 120

Query: 127 RNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAGSFTYLS 186
           ++ PFNL +QV+QFLQFQS SLELI KGYKDLVD EGF NA+YTIDIGQNDLAGSFTYLS
Sbjct: 121 KDRPFNLYIQVMQFLQFQSRSLELIPKGYKDLVDEEGFNNAIYTIDIGQNDLAGSFTYLS 180

Query: 187 YPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHGC 246
           Y QVI RIPSFV+EI++AIW+IYQHGGRNFWVHNTGPLGC+PQKLAT + NASDIDNHGC
Sbjct: 181 YSQVIHRIPSFVTEIKNAIWTIYQHGGRNFWVHNTGPLGCLPQKLATASTNASDIDNHGC 240

Query: 247 LQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVCC 306
           LQ+LNNAAKEFNT LKAACGELRSAL NATIVYVDVYA+KY+LIAN+  NGFENP   CC
Sbjct: 241 LQSLNNAAKEFNTLLKAACGELRSALMNATIVYVDVYAIKYDLIANAVLNGFENPSTACC 300

Query: 307 GYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYSTPNFH 366
           GYGGPPYNFNQS+TCG  GFNVC+EGLKY+SWDGVHYTEAAN VFASKILSS+YS+P  H
Sbjct: 301 GYGGPPYNFNQSVTCGAPGFNVCNEGLKYVSWDGVHYTEAANGVFASKILSSQYSSPKLH 360

Query: 367 FNFFCN 373
           FN+FCN
Sbjct: 361 FNYFCN 366

BLAST of MS018955 vs. NCBI nr
Match: XP_008447456.1 (PREDICTED: GDSL esterase/lipase At1g09390-like [Cucumis melo])

HSP 1 Score: 654.8 bits (1688), Expect = 4.4e-184
Identity = 310/374 (82.89%), Postives = 339/374 (90.64%), Query Frame = 0

Query: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEG 60
           MNLITEM E+ R SFPF LFVIFFLLSLP PL VHSQ  QKPVIFNFGDSNSDTGG SEG
Sbjct: 1   MNLITEMEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEG 60

Query: 61  LGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120
           LG++FG P GRTFFHKP+GRLCDGRLMIDFLCESVSSDYLTPYLRSVG NFT+GANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180
           GS+TLP++ PFNL VQ++QFLQFQS SLELI KGYKDLVD EGF NALYTIDIGQNDLA 
Sbjct: 121 GSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAA 180

Query: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLAT--TAANA 240
           +FTYL Y QVIQ+IPSFVSEI+DAIW++YQHGGR FW+HNTGPLGC+PQKLA     ANA
Sbjct: 181 AFTYLPYSQVIQKIPSFVSEIKDAIWNLYQHGGRKFWIHNTGPLGCLPQKLAAAYANANA 240

Query: 241 SDIDNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGF 300
           SDIDNHGCLQ+LNNAAKEFNTQL+ ACGELRSALTN TIVYVDVYA+KY++IANS SNGF
Sbjct: 241 SDIDNHGCLQSLNNAAKEFNTQLRVACGELRSALTNVTIVYVDVYAIKYDIIANSVSNGF 300

Query: 301 ENPLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSS 360
           ENPL+VCCGYGGPPYNFNQS+TCGQ GFN C+EGLKY+SWDGVHYTEAANAVFASKILSS
Sbjct: 301 ENPLIVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYVSWDGVHYTEAANAVFASKILSS 360

Query: 361 EYSTPNFHFNFFCN 373
           +YS+P   FNFFC+
Sbjct: 361 QYSSPKLPFNFFCS 374

BLAST of MS018955 vs. NCBI nr
Match: XP_023525460.1 (GDSL esterase/lipase At1g09390-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.4 bits (1687), Expect = 5.7e-184
Identity = 306/366 (83.61%), Postives = 333/366 (90.98%), Query Frame = 0

Query: 7   MAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFG 66
           M EK R SFPF LFV+FF+LSLP PL V SQ SQKPVIFNFGDSNSDTGG+SEGLGLRFG
Sbjct: 1   MTEKKRNSFPFPLFVMFFVLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFG 60

Query: 67  PPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAISGSSTLP 126
           PP+GR FFHKPSGRLCDGRLMIDFLCESV SDYLTPYLRSVGSNFT+G NFAISGS+TLP
Sbjct: 61  PPSGRVFFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLP 120

Query: 127 RNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAGSFTYLS 186
           ++ PFNL +QV+QFLQFQS SLE I KGYKDLVD EGF NA+YTIDIGQNDLAGSFTYLS
Sbjct: 121 KDRPFNLYIQVMQFLQFQSRSLEFIPKGYKDLVDEEGFNNAIYTIDIGQNDLAGSFTYLS 180

Query: 187 YPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHGC 246
           Y QVIQRIPSFV+EI++AIW+IYQHGGRNFWVHNTGPLGC+PQKLAT + NASDIDNHGC
Sbjct: 181 YSQVIQRIPSFVTEIKNAIWTIYQHGGRNFWVHNTGPLGCLPQKLATASTNASDIDNHGC 240

Query: 247 LQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVCC 306
           LQ+LNNAAKEFNT LKAACGELRSAL NATIVYVD+YA+KY+LIAN+  NGFENP   CC
Sbjct: 241 LQSLNNAAKEFNTLLKAACGELRSALMNATIVYVDIYAIKYDLIANAVLNGFENPSTACC 300

Query: 307 GYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYSTPNFH 366
           GYGGPPYNFNQS+TCG  GFNVC+EGLKY+SWDGVHYTEAAN VFASKILSS+YS+P  H
Sbjct: 301 GYGGPPYNFNQSVTCGMPGFNVCNEGLKYVSWDGVHYTEAANGVFASKILSSQYSSPKLH 360

Query: 367 FNFFCN 373
           FNFFCN
Sbjct: 361 FNFFCN 366

BLAST of MS018955 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.9e-115
Identity = 212/370 (57.30%), Postives = 263/370 (71.08%), Query Frame = 0

Query: 7   MAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFG 66
           MA  +  S  F L ++ F+L L   L V       PVIFNFGDSNSDTGG   GLG   G
Sbjct: 1   MATLSLHSHSFLLVLLPFILILRQNLAVAGGCQVPPVIFNFGDSNSDTGGLVAGLGYSIG 60

Query: 67  PPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRS-VGSNFTDGANFAISGSSTL 126
            PNGR+FF + +GRL DGRL+IDFLC+S+++  L PYL S VGS F +GANFAI GSSTL
Sbjct: 61  LPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTL 120

Query: 127 PRNHPFNLNVQVLQFLQFQSCSLEL--ISKGYKD-LVDVEGFKNALYTIDIGQNDLAGSF 186
           PR  PF LN+Q++QFL F+S +LEL  IS   K+ ++   GF+NALY IDIGQND+A SF
Sbjct: 121 PRYVPFALNIQLMQFLHFKSRALELASISDPLKEMMIGESGFRNALYMIDIGQNDIADSF 180

Query: 187 TY-LSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDI 246
           +  LSY +V++ IP+ +SEI+ AI  +Y  GGR FWVHNTGPLGC+PQKL  +  ++   
Sbjct: 181 SKGLSYSRVVKLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKL--SMVHSKGF 240

Query: 247 DNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENP 306
           D HGCL   N AAK FN  L   C +LR+ L  A IVYVD+YA+KY+LIANS + GFE P
Sbjct: 241 DKHGCLATYNAAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYGFEKP 300

Query: 307 LMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYS 366
           LM CCGYGGPPYN+N +ITCG  G   CDEG ++ISWDG+HYTE ANA+ A K+LS ++S
Sbjct: 301 LMACCGYGGPPYNYNVNITCGNGGSKSCDEGSRFISWDGIHYTETANAIVAMKVLSMQHS 360

Query: 367 TPNFHFNFFC 372
           TP   F+FFC
Sbjct: 361 TPPTPFHFFC 368

BLAST of MS018955 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 9.4e-113
Identity = 205/368 (55.71%), Postives = 256/368 (69.57%), Query Frame = 0

Query: 11  NRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNG 70
           N  SF F   V   LL L  P R  S  + +PVIFNFGDSNSDTGG   GLG   G PNG
Sbjct: 9   NTFSFFFITLVSLALLILRQPSRAAS-CTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNG 68

Query: 71  RTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVG-SNFTDGANFAISGSSTLPRNH 130
           R FF + +GRL DGRL+IDFLC+S+++  L PYL S+G + F +GANFAI+GS TLP+N 
Sbjct: 69  RLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV 128

Query: 131 PFNLNVQVLQFLQFQSCSLELISKGYK---DLVDVEGFKNALYTIDIGQNDLAGSFTY-L 190
           PF+LN+QV QF  F+S SLEL S         +   GFKNALY IDIGQND+A SF    
Sbjct: 129 PFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGN 188

Query: 191 SYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHG 250
           SY Q ++ IP  ++EI+ +I  +Y  GGR FW+HNTGPLGC+PQKL  +   + D+D HG
Sbjct: 189 SYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKL--SMVKSKDLDQHG 248

Query: 251 CLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVC 310
           CL + N+AA  FN  L   C ELR+ L +ATI+Y+D+YA+KY+LIANS   GF++PLM C
Sbjct: 249 CLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMAC 308

Query: 311 CGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYSTPNF 370
           CGYGG PYN+N  ITCG  G NVC+EG ++ISWDG+HYTE ANA+ A K+LS  YS P  
Sbjct: 309 CGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPPT 368

Query: 371 HFNFFCNK 374
            F+FFC +
Sbjct: 369 PFHFFCRR 373

BLAST of MS018955 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 4.2e-97
Identity = 171/327 (52.29%), Postives = 226/327 (69.11%), Query Frame = 0

Query: 39  SQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSD 98
           ++KP++ NFGDSNSDTGG   G+GL  G P+G TFFH+ +GRL DGRL++DF CE +   
Sbjct: 32  NKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGRLGDGRLIVDFYCEHLKMT 91

Query: 99  YLTPYLRSVGSNFTDGANFAISGSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDL 158
           YL+PYL S+  NF  G NFA+SG++ LP    F L +Q+ QF+ F++ S ELIS G +DL
Sbjct: 92  YLSPYLDSLSPNFKRGVNFAVSGATALP-IFSFPLAIQIRQFVHFKNRSQELISSGRRDL 151

Query: 159 VDVEGFKNALYTIDIGQNDLAGSF--TYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNF 218
           +D  GF+NALY IDIGQNDL  +   + L+Y  V+++IPS + EI+ AI ++Y +GGR F
Sbjct: 152 IDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSMLLEIKKAIQTVYLYGGRKF 211

Query: 219 WVHNTGPLGCMPQKLATTAANASDIDNHGCLQALNNAAKEFNTQLKAACGELRSALTNAT 278
           WVHNTGPLGC P++LA    N SD+D  GC +  N  AK FN  L + C ELRS   +AT
Sbjct: 212 WVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAFNKGLLSLCNELRSQFKDAT 271

Query: 279 IVYVDVYAMKYNLIANSASNGFENPLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYI 338
           +VYVD+Y++KY L A+    GF +PLM CCGYGG P N+++  TCGQ G  +C +  K I
Sbjct: 272 LVYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDRKATCGQPGSTICRDVTKAI 331

Query: 339 SWDGVHYTEAANAVFASKILSSEYSTP 364
            WDGVHYTEAAN      +L++ YS P
Sbjct: 332 VWDGVHYTEAANRFVVDAVLTNRYSYP 357

BLAST of MS018955 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.5e-81
Identity = 167/359 (46.52%), Postives = 222/359 (61.84%), Query Frame = 0

Query: 18  SLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNGRTFFHKP 77
           SLF +  L SL      H      P IFNFGDSNSDTGG S   G + GPP+G +FF  P
Sbjct: 7   SLFALSLLSSLSPSTHAHQ--CHFPAIFNFGDSNSDTGGLSAAFG-QAGPPHGSSFFGSP 66

Query: 78  SGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAISGS------STLPRN--H 137
           +GR CDGRL+IDF+ ES+   YL+ +L SVGSNF+ GANFA +GS      STL ++   
Sbjct: 67  AGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFS 126

Query: 138 PFNLNVQVLQFLQFQSCSLELISKG--YKDLV-DVEGFKNALYTIDIGQNDL-AGSFTYL 197
           PF+L+VQ +QF  F + S  + S+G  YK ++ + + F  ALYT DIGQNDL AG F   
Sbjct: 127 PFSLDVQFVQFYNFHNRSQTVRSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANK 186

Query: 198 SYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHG 257
           +  QV   +P  +S+  +AI +IY  GGR FW+HNTGP+GC+   +      ASD D+HG
Sbjct: 187 TVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHG 246

Query: 258 CLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVC 317
           C+  LN+ A++FN  LK A  ELRS+L+ A I YVDVY++K+ L  ++  +GF+  L+ C
Sbjct: 247 CVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSC 306

Query: 318 CGYGGPPYNFNQSITCGQTGF---------NVCDEGLKYISWDGVHYTEAANAVFASKI 356
           CG+GG  YN+N+ I CG               CDE  K + WDGVH+T+AAN     KI
Sbjct: 307 CGHGG-KYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKI 361

BLAST of MS018955 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 4.2e-81
Identity = 161/388 (41.49%), Postives = 227/388 (58.51%), Query Frame = 0

Query: 21  VIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNGRTFFHKPSGR 80
           ++FF+ SL     +   +   P  FNFGDSNSDTG    GLG+R   PNG+  F   S R
Sbjct: 12  ILFFISSLQISNSIDFNY---PSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 71

Query: 81  LCDGRLMIDFLCESVSSDYLTPYLRSVG-SNFTDGANFAISGSSTLPRN----HPFNLNV 140
            CDGRL+IDFL + +   +L PYL S+G  NF  G NFA +GS+ LP N     PF+ ++
Sbjct: 72  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 131

Query: 141 QVLQFLQFQSCSLELISKG----YKDLVDVEGFKNALYTIDIGQNDLAGSFTYLSYPQVI 200
           Q+ QF++F+S ++EL+SK      K L  ++ +   LY IDIGQND+AG+F   +  QV+
Sbjct: 132 QISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVL 191

Query: 201 QRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHGCLQALN 260
             IPS +      +  +Y+ GGRN W+HNTGPLGC+ Q +A    +++ +D  GC+ + N
Sbjct: 192 ASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHN 251

Query: 261 NAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSAS---------------- 320
            AAK FN QL A   + ++   +A + YVD++++K NLIAN +                 
Sbjct: 252 QAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNHLE 311

Query: 321 ----------NGFENPLMVCCGYGGPPYNFNQSITCGQT----GFNV----CDEGLKYIS 366
                      GFE PLM CCG GG P N++  ITCGQT    G +V    C++  +YI+
Sbjct: 312 NVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYIN 371

BLAST of MS018955 vs. ExPASy TrEMBL
Match: A0A0A0L9E0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G669600 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 6.6e-186
Identity = 310/375 (82.67%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEG 60
           MNLITEM E+ R S PF LF+IFFLLSLPDPL VHSQ  QKPVIFNFGDSNSDTGG+SEG
Sbjct: 1   MNLITEMEEEKRDSSPFPLFLIFFLLSLPDPLFVHSQCIQKPVIFNFGDSNSDTGGFSEG 60

Query: 61  LGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120
           LG++FG P GRTFFHKP+GRLCDGRLMIDFLCESV+SDYLTPYLRSVG NFT+GANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVNSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180
           GS+TLP++ PFNL +Q++QFLQFQS SLELI KGYKDLVD EGF NALYTIDIGQNDLA 
Sbjct: 121 GSATLPKDRPFNLYIQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAA 180

Query: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLAT--TAANA 240
           +FTYLSYPQVIQ+IPSFVSEI++AIW+IYQHGGRNFW+HNTGPLGC+PQKLAT    ANA
Sbjct: 181 AFTYLSYPQVIQQIPSFVSEIKNAIWTIYQHGGRNFWIHNTGPLGCLPQKLATAYADANA 240

Query: 241 SDIDNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGF 300
           SDIDNHGCLQ+ NNAAKEFNTQL+ ACGELRSALTNAT+VYVDVYA+KY+LI NS SNGF
Sbjct: 241 SDIDNHGCLQSFNNAAKEFNTQLRVACGELRSALTNATLVYVDVYAIKYDLITNSVSNGF 300

Query: 301 ENPLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSS 360
           ENPL+VCCGYGGPPYNFNQ++TCGQ GFN C+EGLKY+SWDGVHYTEAANAVFAS ILSS
Sbjct: 301 ENPLIVCCGYGGPPYNFNQTVTCGQPGFNTCNEGLKYVSWDGVHYTEAANAVFASMILSS 360

Query: 361 EYSTPNFHFNFFCNK 374
           +YS+P   FNFFCNK
Sbjct: 361 QYSSPKLPFNFFCNK 375

BLAST of MS018955 vs. ExPASy TrEMBL
Match: A0A6J1KA99 (GDSL esterase/lipase At1g09390-like OS=Cucurbita maxima OX=3661 GN=LOC111493087 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 308/366 (84.15%), Postives = 333/366 (90.98%), Query Frame = 0

Query: 7   MAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFG 66
           M EK R SFPF LFVIFFLLSLP PL V SQ SQKPVIFNFGDSNSDTGG+SEGLGLRFG
Sbjct: 1   MTEKKRNSFPFPLFVIFFLLSLPGPLLVLSQCSQKPVIFNFGDSNSDTGGFSEGLGLRFG 60

Query: 67  PPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAISGSSTLP 126
           PP+GR FFHKPSGRLCDGRLMIDFLCESV SDYLTPYLRSVGSNFT+G NFAISGS+TLP
Sbjct: 61  PPSGRVFFHKPSGRLCDGRLMIDFLCESVGSDYLTPYLRSVGSNFTNGVNFAISGSATLP 120

Query: 127 RNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAGSFTYLS 186
           ++ PFNL +QV+QFLQFQS SLELI KGYKDLVD EGF NA+YTIDIGQNDLAGSFTYLS
Sbjct: 121 KDRPFNLYIQVMQFLQFQSRSLELIPKGYKDLVDEEGFNNAIYTIDIGQNDLAGSFTYLS 180

Query: 187 YPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHGC 246
           Y QVI RIPSFV+EI++AIW+IYQHGGRNFWVHNTGPLGC+PQKLAT + NASDIDNHGC
Sbjct: 181 YSQVIHRIPSFVTEIKNAIWTIYQHGGRNFWVHNTGPLGCLPQKLATASTNASDIDNHGC 240

Query: 247 LQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVCC 306
           LQ+LNNAAKEFNT LKAACGELRSAL NATIVYVDVYA+KY+LIAN+  NGFENP   CC
Sbjct: 241 LQSLNNAAKEFNTLLKAACGELRSALMNATIVYVDVYAIKYDLIANAVLNGFENPSTACC 300

Query: 307 GYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYSTPNFH 366
           GYGGPPYNFNQS+TCG  GFNVC+EGLKY+SWDGVHYTEAAN VFASKILSS+YS+P  H
Sbjct: 301 GYGGPPYNFNQSVTCGAPGFNVCNEGLKYVSWDGVHYTEAANGVFASKILSSQYSSPKLH 360

Query: 367 FNFFCN 373
           FN+FCN
Sbjct: 361 FNYFCN 366

BLAST of MS018955 vs. ExPASy TrEMBL
Match: A0A1S3BHH9 (GDSL esterase/lipase At1g09390-like OS=Cucumis melo OX=3656 GN=LOC103489900 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.1e-184
Identity = 310/374 (82.89%), Postives = 339/374 (90.64%), Query Frame = 0

Query: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEG 60
           MNLITEM E+ R SFPF LFVIFFLLSLP PL VHSQ  QKPVIFNFGDSNSDTGG SEG
Sbjct: 1   MNLITEMEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEG 60

Query: 61  LGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120
           LG++FG P GRTFFHKP+GRLCDGRLMIDFLCESVSSDYLTPYLRSVG NFT+GANFAIS
Sbjct: 61  LGIKFGLPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAIS 120

Query: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180
           GS+TLP++ PFNL VQ++QFLQFQS SLELI KGYKDLVD EGF NALYTIDIGQNDLA 
Sbjct: 121 GSATLPKDRPFNLYVQIMQFLQFQSRSLELIPKGYKDLVDEEGFNNALYTIDIGQNDLAA 180

Query: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLAT--TAANA 240
           +FTYL Y QVIQ+IPSFVSEI+DAIW++YQHGGR FW+HNTGPLGC+PQKLA     ANA
Sbjct: 181 AFTYLPYSQVIQKIPSFVSEIKDAIWNLYQHGGRKFWIHNTGPLGCLPQKLAAAYANANA 240

Query: 241 SDIDNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGF 300
           SDIDNHGCLQ+LNNAAKEFNTQL+ ACGELRSALTN TIVYVDVYA+KY++IANS SNGF
Sbjct: 241 SDIDNHGCLQSLNNAAKEFNTQLRVACGELRSALTNVTIVYVDVYAIKYDIIANSVSNGF 300

Query: 301 ENPLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSS 360
           ENPL+VCCGYGGPPYNFNQS+TCGQ GFN C+EGLKY+SWDGVHYTEAANAVFASKILSS
Sbjct: 301 ENPLIVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYVSWDGVHYTEAANAVFASKILSS 360

Query: 361 EYSTPNFHFNFFCN 373
           +YS+P   FNFFC+
Sbjct: 361 QYSSPKLPFNFFCS 374

BLAST of MS018955 vs. ExPASy TrEMBL
Match: A0A5A7T9T8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00720 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.1e-179
Identity = 304/371 (81.94%), Postives = 333/371 (89.76%), Query Frame = 0

Query: 7   MAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFG 66
           M E+ R SFPF LFVIFFLLSLP PL VHSQ  QKPVIFNFGDSNSDTGG SEGLG++FG
Sbjct: 1   MEEEKRTSFPFPLFVIFFLLSLPGPLFVHSQCIQKPVIFNFGDSNSDTGGLSEGLGIKFG 60

Query: 67  PPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAISGSSTLP 126
            P GRTFFHKP+GRLCDGRLMIDFLCESVSSDYLTPYLRSVG NFT+GANFAISGS+TLP
Sbjct: 61  LPTGRTFFHKPAGRLCDGRLMIDFLCESVSSDYLTPYLRSVGPNFTNGANFAISGSATLP 120

Query: 127 RNHPFNLNVQVLQFLQFQSCSLELISKG---YKDLVDVEGFKNALYTIDIGQNDLAGSFT 186
           ++ PFNL VQ++QFLQFQS SLELI KG   YKDLVD EGF NALYTIDIGQNDLA +FT
Sbjct: 121 KDRPFNLYVQIMQFLQFQSRSLELIPKGIPRYKDLVDEEGFNNALYTIDIGQNDLAAAFT 180

Query: 187 YLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLAT--TAANASDI 246
           YL Y QVIQ+IPSFVSEI+DAIW++YQHGGR FW+HNTGPLGC+PQKLA     ANASDI
Sbjct: 181 YLPYSQVIQKIPSFVSEIKDAIWNLYQHGGRKFWIHNTGPLGCLPQKLAAAYANANASDI 240

Query: 247 DNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENP 306
           DNHGCLQ+LNNAAKEFNTQL+ ACGELRSALTN TIVYVDVYA+KY++IANS SNGFENP
Sbjct: 241 DNHGCLQSLNNAAKEFNTQLRVACGELRSALTNVTIVYVDVYAIKYDIIANSVSNGFENP 300

Query: 307 LMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYS 366
           L+VCCGYGGPPYNFNQS+TCGQ GFN C+EGLKY+SWDGVHYTEAANAVFASKILSS+YS
Sbjct: 301 LIVCCGYGGPPYNFNQSVTCGQPGFNTCNEGLKYVSWDGVHYTEAANAVFASKILSSQYS 360

Query: 367 TPNFHFNFFCN 373
           +P   FNFFC+
Sbjct: 361 SPKLPFNFFCS 371

BLAST of MS018955 vs. ExPASy TrEMBL
Match: A0A6J1DSA1 (GDSL esterase/lipase At1g09390-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023837 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.1e-171
Identity = 298/304 (98.03%), Postives = 300/304 (98.68%), Query Frame = 0

Query: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEG 60
           MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQ S+KPVIFNFGDSNSDTGGYSEG
Sbjct: 1   MNLITEMAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQCSRKPVIFNFGDSNSDTGGYSEG 60

Query: 61  LGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120
           LGLRFGPPNG TFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS
Sbjct: 61  LGLRFGPPNGXTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVGSNFTDGANFAIS 120

Query: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180
           GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG
Sbjct: 121 GSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDLVDVEGFKNALYTIDIGQNDLAG 180

Query: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASD 240
           SFTYLSYPQVIQRIPSFVSEIRDAIW IYQHGGRNFWVHNTGPLGCMPQKLATTAANASD
Sbjct: 181 SFTYLSYPQVIQRIPSFVSEIRDAIWXIYQHGGRNFWVHNTGPLGCMPQKLATTAANASD 240

Query: 241 IDNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFEN 300
           IDNHGCLQALNNAAKEFN QLKAACGELRSALTNATIVYVDVYA+KYNLIANSASNGFEN
Sbjct: 241 IDNHGCLQALNNAAKEFNXQLKAACGELRSALTNATIVYVDVYAIKYNLIANSASNGFEN 300

Query: 301 PLMV 305
           PLMV
Sbjct: 301 PLMV 304

BLAST of MS018955 vs. TAIR 10
Match: AT1G09390.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 415.6 bits (1067), Expect = 4.2e-116
Identity = 212/370 (57.30%), Postives = 263/370 (71.08%), Query Frame = 0

Query: 7   MAEKNRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFG 66
           MA  +  S  F L ++ F+L L   L V       PVIFNFGDSNSDTGG   GLG   G
Sbjct: 1   MATLSLHSHSFLLVLLPFILILRQNLAVAGGCQVPPVIFNFGDSNSDTGGLVAGLGYSIG 60

Query: 67  PPNGRTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRS-VGSNFTDGANFAISGSSTL 126
            PNGR+FF + +GRL DGRL+IDFLC+S+++  L PYL S VGS F +GANFAI GSSTL
Sbjct: 61  LPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTL 120

Query: 127 PRNHPFNLNVQVLQFLQFQSCSLEL--ISKGYKD-LVDVEGFKNALYTIDIGQNDLAGSF 186
           PR  PF LN+Q++QFL F+S +LEL  IS   K+ ++   GF+NALY IDIGQND+A SF
Sbjct: 121 PRYVPFALNIQLMQFLHFKSRALELASISDPLKEMMIGESGFRNALYMIDIGQNDIADSF 180

Query: 187 TY-LSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDI 246
           +  LSY +V++ IP+ +SEI+ AI  +Y  GGR FWVHNTGPLGC+PQKL  +  ++   
Sbjct: 181 SKGLSYSRVVKLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKL--SMVHSKGF 240

Query: 247 DNHGCLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENP 306
           D HGCL   N AAK FN  L   C +LR+ L  A IVYVD+YA+KY+LIANS + GFE P
Sbjct: 241 DKHGCLATYNAAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYGFEKP 300

Query: 307 LMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYS 366
           LM CCGYGGPPYN+N +ITCG  G   CDEG ++ISWDG+HYTE ANA+ A K+LS ++S
Sbjct: 301 LMACCGYGGPPYNYNVNITCGNGGSKSCDEGSRFISWDGIHYTETANAIVAMKVLSMQHS 360

Query: 367 TPNFHFNFFC 372
           TP   F+FFC
Sbjct: 361 TPPTPFHFFC 368

BLAST of MS018955 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 6.6e-114
Identity = 205/368 (55.71%), Postives = 256/368 (69.57%), Query Frame = 0

Query: 11  NRKSFPFSLFVIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNG 70
           N  SF F   V   LL L  P R  S  + +PVIFNFGDSNSDTGG   GLG   G PNG
Sbjct: 9   NTFSFFFITLVSLALLILRQPSRAAS-CTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNG 68

Query: 71  RTFFHKPSGRLCDGRLMIDFLCESVSSDYLTPYLRSVG-SNFTDGANFAISGSSTLPRNH 130
           R FF + +GRL DGRL+IDFLC+S+++  L PYL S+G + F +GANFAI+GS TLP+N 
Sbjct: 69  RLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV 128

Query: 131 PFNLNVQVLQFLQFQSCSLELISKGYK---DLVDVEGFKNALYTIDIGQNDLAGSFTY-L 190
           PF+LN+QV QF  F+S SLEL S         +   GFKNALY IDIGQND+A SF    
Sbjct: 129 PFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGN 188

Query: 191 SYPQVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHG 250
           SY Q ++ IP  ++EI+ +I  +Y  GGR FW+HNTGPLGC+PQKL  +   + D+D HG
Sbjct: 189 SYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKL--SMVKSKDLDQHG 248

Query: 251 CLQALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVC 310
           CL + N+AA  FN  L   C ELR+ L +ATI+Y+D+YA+KY+LIANS   GF++PLM C
Sbjct: 249 CLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMAC 308

Query: 311 CGYGGPPYNFNQSITCGQTGFNVCDEGLKYISWDGVHYTEAANAVFASKILSSEYSTPNF 370
           CGYGG PYN+N  ITCG  G NVC+EG ++ISWDG+HYTE ANA+ A K+LS  YS P  
Sbjct: 309 CGYGGTPYNYNVKITCGHKGSNVCEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPPT 368

Query: 371 HFNFFCNK 374
            F+FFC +
Sbjct: 369 PFHFFCRR 373

BLAST of MS018955 vs. TAIR 10
Match: AT3G62280.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 3.0e-98
Identity = 171/327 (52.29%), Postives = 226/327 (69.11%), Query Frame = 0

Query: 39  SQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNGRTFFHKPSGRLCDGRLMIDFLCESVSSD 98
           ++KP++ NFGDSNSDTGG   G+GL  G P+G TFFH+ +GRL DGRL++DF CE +   
Sbjct: 32  NKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGRLGDGRLIVDFYCEHLKMT 91

Query: 99  YLTPYLRSVGSNFTDGANFAISGSSTLPRNHPFNLNVQVLQFLQFQSCSLELISKGYKDL 158
           YL+PYL S+  NF  G NFA+SG++ LP    F L +Q+ QF+ F++ S ELIS G +DL
Sbjct: 92  YLSPYLDSLSPNFKRGVNFAVSGATALP-IFSFPLAIQIRQFVHFKNRSQELISSGRRDL 151

Query: 159 VDVEGFKNALYTIDIGQNDLAGSF--TYLSYPQVIQRIPSFVSEIRDAIWSIYQHGGRNF 218
           +D  GF+NALY IDIGQNDL  +   + L+Y  V+++IPS + EI+ AI ++Y +GGR F
Sbjct: 152 IDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSMLLEIKKAIQTVYLYGGRKF 211

Query: 219 WVHNTGPLGCMPQKLATTAANASDIDNHGCLQALNNAAKEFNTQLKAACGELRSALTNAT 278
           WVHNTGPLGC P++LA    N SD+D  GC +  N  AK FN  L + C ELRS   +AT
Sbjct: 212 WVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAFNKGLLSLCNELRSQFKDAT 271

Query: 279 IVYVDVYAMKYNLIANSASNGFENPLMVCCGYGGPPYNFNQSITCGQTGFNVCDEGLKYI 338
           +VYVD+Y++KY L A+    GF +PLM CCGYGG P N+++  TCGQ G  +C +  K I
Sbjct: 272 LVYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDRKATCGQPGSTICRDVTKAI 331

Query: 339 SWDGVHYTEAANAVFASKILSSEYSTP 364
            WDGVHYTEAAN      +L++ YS P
Sbjct: 332 VWDGVHYTEAANRFVVDAVLTNRYSYP 357

BLAST of MS018955 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-86
Identity = 161/362 (44.48%), Postives = 227/362 (62.71%), Query Frame = 0

Query: 21  VIFFLLSLPDPLRVHSQFSQKPVIFNFGDSNSDTGGYSEGLGLRFGPPNGRTFFHKPSGR 80
           ++FF+ SL     +   +   P  FNFGDSNSDTG    GLG+R   PNG+  F   S R
Sbjct: 12  ILFFISSLQISNSIDFNY---PSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 71

Query: 81  LCDGRLMIDFLCESVSSDYLTPYLRSVG-SNFTDGANFAISGSSTLPRN----HPFNLNV 140
            CDGRL+IDFL + +   +L PYL S+G  NF  G NFA +GS+ LP N     PF+ ++
Sbjct: 72  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 131

Query: 141 QVLQFLQFQSCSLELISKG----YKDLVDVEGFKNALYTIDIGQNDLAGSFTYLSYPQVI 200
           Q+ QF++F+S ++EL+SK      K L  ++ +   LY IDIGQND+AG+F   +  QV+
Sbjct: 132 QISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVL 191

Query: 201 QRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHGCLQALN 260
             IPS +      +  +Y+ GGRN W+HNTGPLGC+ Q +A    +++ +D  GC+ + N
Sbjct: 192 ASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHN 251

Query: 261 NAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVCCGYGGP 320
            AAK FN QL A   + ++   +A + YVD++++K NLIAN +  GFE PLM CCG GG 
Sbjct: 252 QAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGGA 311

Query: 321 PYNFNQSITCGQT----GFNV----CDEGLKYISWDGVHYTEAANAVFASKILSSEYSTP 366
           P N++  ITCGQT    G +V    C++  +YI+WDG+HYTEAAN   +S+IL+ +YS P
Sbjct: 312 PLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDP 370

BLAST of MS018955 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-86
Identity = 164/365 (44.93%), Postives = 230/365 (63.01%), Query Frame = 0

Query: 21  VIFFLLSLPDPLRVHSQFS---QKPVIFNFGDSNSDTGGYSEGLGLRFGPPNGRTFFHKP 80
           V+F L+SL  P    S FS   + P I NFGDSNSDTG           PP G+T+F+ P
Sbjct: 11  VLFILISLFLP----SSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLP 70

Query: 81  SGRLCDGRLMIDFLCESVSSDYLTPYLRSVG-SNFTDGANFAISGSSTLPRN----HPFN 140
           SGR CDGRL++DFL + +   +L PYL S+G  NF  G NFA +GS+ LP N     PF+
Sbjct: 71  SGRYCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFS 130

Query: 141 LNVQVLQFLQFQSCSLELISKG----YKDLVDVEGFKNALYTIDIGQNDLAGSFTYLSYP 200
            ++Q+ QF++F+S ++EL+SK      K L  ++ +   LY IDIGQND+AG+F   +  
Sbjct: 131 FDLQISQFIRFKSRAIELLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLD 190

Query: 201 QVIQRIPSFVSEIRDAIWSIYQHGGRNFWVHNTGPLGCMPQKLATTAANASDIDNHGCLQ 260
           QV+  IPS +      +  +Y+ GGRN W+HNTGPLGC+ Q +A    +++ +D  GC+ 
Sbjct: 191 QVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVS 250

Query: 261 ALNNAAKEFNTQLKAACGELRSALTNATIVYVDVYAMKYNLIANSASNGFENPLMVCCGY 320
           + N AAK FN QL A   + ++   +A + YVD++++K NLIAN +  GFE PLM CCG 
Sbjct: 251 SHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGV 310

Query: 321 GGPPYNFNQSITCGQT----GFNV----CDEGLKYISWDGVHYTEAANAVFASKILSSEY 366
           GG P N++  ITCGQT    G +V    C++  +YI+WDG+HYTEAAN   +S+IL+ +Y
Sbjct: 311 GGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKY 370