MS018951 (gene) Bitter gourd (TR) v1

Overview


Name	MS018951
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold20: 144140 .. 146444 (+)
RNA-Seq Expression	MS018951
Synteny	MS018951

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGTGTCATATTTCTTCATTTTGTTTTTGCATTCGAATTTTCATCTTCTTCTGCCTCCTGTTTTCTGTCCATGGCCGCCATGCAAAGAAGCACAAACATTCTCACCATCATGATCATTACTCCATTTCACAGCCTCCTTCTCCTCCTCCTTCTCCTTCGTCGGGATTGGCAAGTCCTCCCGACGATGGAGGGTACTCCTCGTCCTTGAACTCCAGCGGCGGCATTTTTGACGTACGTCATTTTGGTGCCATTGGAGATGGTATGGTGGACGACACGGAGGCGTTCAAGATGGCGTGGGACACAGCATGCCAGAGTGATGATGATGATAGCTCTGCAGTTATTCTCGTTCCCTACGGTTTCTCCTTTTTGATTCAGTCGACAATTTTCACAGGGCCGTGCAAAAAAGGGGTTGTGTTTCACATTGATGGCACTCTTGTAGCTCCCGATGGGCCCGATGCTTGGCCGAGGAGTTATAGTGCGCATCAATGGTTGGTTTTCTACCGAGTTTATAACATGTCGCTTCGAGGTGATGGTCTCATTGATGGTAGAGGCCAGAAATGGTGGGATCTCCCTTGCAAACCCCACAAGGTAAGAGAGATTCTTTCACCCATTTTTTTTAATATCACACGAATCTAGAATGTTTAAATATGGGAAGAGAAGGAGTGAAGGAATATTTTTTAAATACATCTCAGGTTCTAAGACTACTTTATTTTTGTAGGGAGGAACAGGAAAAACGACAACGGGACGATGTGATAGCCCAATTGTAAGTCAAAGTTGTGTTATAAAATCTTGTTTATGTTTTTTTTATGAGTTTTTAAAAAGTTAGGGTTTTGCAGGCAATAAGATTCTTCATGAGCTCCAACTTGACAGTGGAAGGACTTAGAATAAGAGACAGTCCTCAGTTTCACTTCAGGTTTGATAATTGCAGAGATGTTTATATTGATTCTCTCCAAATAACAGCTCCTGCCTTCAGTCCAAACACTGATGGAATTCACATTGAGAATACAAATGGTGTCAAGATATACAATTCAATAGTTGCCAATGGTTTGTACTCGGTCATTTTCTTAATCGTTTGTTTTCTGTTCAATAAGTGGAACGTATTAGCTGTCCGCTCTTCGGTTGGACAGTGAGGGTCGGAGTAAAAAAGAAGTATGACTTTCATAATCTAACTGTCCTCCTGAACCCATTGAGAAAAATGAGAATTGAAGTCTATGAGACCTAATTTCCTGTCTCTTTGTTTCTTCTCGCGGCAAAAAAGCAAGATTCTCAAACTCCTCTTTTAAAAGTGAAAACATGTTCTAAAATGGGTATTAATTACATAACTTTGTTTAATAATGTCCTCATTTCTTGTATCTGGTAAGATAGAAGAAAAACAAAAAAAATGCATGTTTGACCATTGGTTTTTTTTTTTGGTTTATTTTCTTCTTTGTTTTTTACAAAATAAAAAAAAAAGAATGCCTTTTGGGACAAGAAACAAGAAATCGGTTGTTGGGAGGAAGAGATGTATAAGTTACGTTCGTTAAAATTTGAAAACAAGAAATAAGAAATGATACGAAAAGGAAACTTTCTTTCTTTTCTTTTTTTGGATAACGGTTCCTAACTGTTGTTAGGTGATTTTTTGTACAGGTGATGATTGCATTTCAATTGGATCAGGTTCTTATAATGTTGATATAAAAAATATAACATGTGGACCAGGTCATGGAATTAGGTAATAAAACTTTTGTTTCTTTCAAATATATAGATAGAAGAATTTTATGTTCTTCTTAAAAATACTAGTTATTTACACCTTTGGTTTGAATCTAGCATTGGCAGTCTAGGCAATCACAACTCCCGAGCATGTGTGTCGAACGTAACGGTTCGGGACTCGACAATCAAAGTCTCAGACAATGGGGTTCGAATCAAGACATGGCAAGGCGGATTTGGAGCAGTAAGAGGAGTAAGTTTTAACAACATTCATATGGACAATGTTAGGAATCCCATCATAATTGATCAATTCTACTGCCTCAACAAAGGGTGCCTCAATCAATCCTCAGCAGTTCTTGTATCAGACATAACCTATACCGATATCAAAGGAACTTACGATATTAGGAGCCCACCGTTGCATTTTGGTTGTAGTGACTCCATGCCATGCACAAACTTAACACTTGCTGATATTGAACTTCTTCCCGCTAGAGGCGACATTGTTTCGGACCCCTTTTGTTGGAATGCTTATGGAAACTTGCAAACCCTAACAATTCCTCCCGTTGCTTGCTTACTCGAAAGCGCCGCACGATCGACCATACAAAGTGAAAAGGATTATTGT

mRNA sequence

ATGTTGTGTCATATTTCTTCATTTTGTTTTTGCATTCGAATTTTCATCTTCTTCTGCCTCCTGTTTTCTGTCCATGGCCGCCATGCAAAGAAGCACAAACATTCTCACCATCATGATCATTACTCCATTTCACAGCCTCCTTCTCCTCCTCCTTCTCCTTCGTCGGGATTGGCAAGTCCTCCCGACGATGGAGGGTACTCCTCGTCCTTGAACTCCAGCGGCGGCATTTTTGACGTACGTCATTTTGGTGCCATTGGAGATGGTATGGTGGACGACACGGAGGCGTTCAAGATGGCGTGGGACACAGCATGCCAGAGTGATGATGATGATAGCTCTGCAGTTATTCTCGTTCCCTACGGTTTCTCCTTTTTGATTCAGTCGACAATTTTCACAGGGCCGTGCAAAAAAGGGGTTGTGTTTCACATTGATGGCACTCTTGTAGCTCCCGATGGGCCCGATGCTTGGCCGAGGAGTTATAGTGCGCATCAATGGTTGGTTTTCTACCGAGTTTATAACATGTCGCTTCGAGGTGATGGTCTCATTGATGGTAGAGGCCAGAAATGGTGGGATCTCCCTTGCAAACCCCACAAGGGAGGAACAGGAAAAACGACAACGGGACGATGTGATAGCCCAATTGCAATAAGATTCTTCATGAGCTCCAACTTGACAGTGGAAGGACTTAGAATAAGAGACAGTCCTCAGTTTCACTTCAGGTTTGATAATTGCAGAGATGTTTATATTGATTCTCTCCAAATAACAGCTCCTGCCTTCAGTCCAAACACTGATGGAATTCACATTGAGAATACAAATGGTGTCAAGATATACAATTCAATAGTTGCCAATGGTGATGATTGCATTTCAATTGGATCAGGTTCTTATAATGTTGATATAAAAAATATAACATGTGGACCAGGTCATGGAATTAGCATTGGCAGTCTAGGCAATCACAACTCCCGAGCATGTGTGTCGAACGTAACGGTTCGGGACTCGACAATCAAAGTCTCAGACAATGGGGTTCGAATCAAGACATGGCAAGGCGGATTTGGAGCAGTAAGAGGAGTAAGTTTTAACAACATTCATATGGACAATGTTAGGAATCCCATCATAATTGATCAATTCTACTGCCTCAACAAAGGGTGCCTCAATCAATCCTCAGCAGTTCTTGTATCAGACATAACCTATACCGATATCAAAGGAACTTACGATATTAGGAGCCCACCGTTGCATTTTGGTTGTAGTGACTCCATGCCATGCACAAACTTAACACTTGCTGATATTGAACTTCTTCCCGCTAGAGGCGACATTGTTTCGGACCCCTTTTGTTGGAATGCTTATGGAAACTTGCAAACCCTAACAATTCCTCCCGTTGCTTGCTTACTCGAAAGCGCCGCACGATCGACCATACAAAGTGAAAAGGATTATTGT

Coding sequence (CDS)

ATGTTGTGTCATATTTCTTCATTTTGTTTTTGCATTCGAATTTTCATCTTCTTCTGCCTCCTGTTTTCTGTCCATGGCCGCCATGCAAAGAAGCACAAACATTCTCACCATCATGATCATTACTCCATTTCACAGCCTCCTTCTCCTCCTCCTTCTCCTTCGTCGGGATTGGCAAGTCCTCCCGACGATGGAGGGTACTCCTCGTCCTTGAACTCCAGCGGCGGCATTTTTGACGTACGTCATTTTGGTGCCATTGGAGATGGTATGGTGGACGACACGGAGGCGTTCAAGATGGCGTGGGACACAGCATGCCAGAGTGATGATGATGATAGCTCTGCAGTTATTCTCGTTCCCTACGGTTTCTCCTTTTTGATTCAGTCGACAATTTTCACAGGGCCGTGCAAAAAAGGGGTTGTGTTTCACATTGATGGCACTCTTGTAGCTCCCGATGGGCCCGATGCTTGGCCGAGGAGTTATAGTGCGCATCAATGGTTGGTTTTCTACCGAGTTTATAACATGTCGCTTCGAGGTGATGGTCTCATTGATGGTAGAGGCCAGAAATGGTGGGATCTCCCTTGCAAACCCCACAAGGGAGGAACAGGAAAAACGACAACGGGACGATGTGATAGCCCAATTGCAATAAGATTCTTCATGAGCTCCAACTTGACAGTGGAAGGACTTAGAATAAGAGACAGTCCTCAGTTTCACTTCAGGTTTGATAATTGCAGAGATGTTTATATTGATTCTCTCCAAATAACAGCTCCTGCCTTCAGTCCAAACACTGATGGAATTCACATTGAGAATACAAATGGTGTCAAGATATACAATTCAATAGTTGCCAATGGTGATGATTGCATTTCAATTGGATCAGGTTCTTATAATGTTGATATAAAAAATATAACATGTGGACCAGGTCATGGAATTAGCATTGGCAGTCTAGGCAATCACAACTCCCGAGCATGTGTGTCGAACGTAACGGTTCGGGACTCGACAATCAAAGTCTCAGACAATGGGGTTCGAATCAAGACATGGCAAGGCGGATTTGGAGCAGTAAGAGGAGTAAGTTTTAACAACATTCATATGGACAATGTTAGGAATCCCATCATAATTGATCAATTCTACTGCCTCAACAAAGGGTGCCTCAATCAATCCTCAGCAGTTCTTGTATCAGACATAACCTATACCGATATCAAAGGAACTTACGATATTAGGAGCCCACCGTTGCATTTTGGTTGTAGTGACTCCATGCCATGCACAAACTTAACACTTGCTGATATTGAACTTCTTCCCGCTAGAGGCGACATTGTTTCGGACCCCTTTTGTTGGAATGCTTATGGAAACTTGCAAACCCTAACAATTCCTCCCGTTGCTTGCTTACTCGAAAGCGCCGCACGATCGACCATACAAAGTGAAAAGGATTATTGT

Protein sequence

MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASPPDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC

Homology

[Close]

BLAST of MS018951 vs. NCBI nr
Match: XP_022155236.1 (polygalacturonase At1g48100-like isoform X1 [Momordica charantia])

HSP 1 Score: 993.0 bits (2566), Expect = 8.6e-286
Identity = 473/475 (99.58%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60
           MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP
Sbjct: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60

Query: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120
           PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG
Sbjct: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120

Query: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGL 180
           FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSL+GDGL
Sbjct: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLQGDGL 180

Query: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240
           IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD
Sbjct: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240

Query: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300
           NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI
Sbjct: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300

Query: 301 TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360
           TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH
Sbjct: 301 TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360

Query: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420
           MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN
Sbjct: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420

Query: 421 LTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           LTLADIELLPARGDIVS+PFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC
Sbjct: 421 LTLADIELLPARGDIVSNPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 475

[Close]

BLAST of MS018951 vs. NCBI nr
Match: XP_022155237.1 (polygalacturonase At1g48100-like isoform X2 [Momordica charantia])

HSP 1 Score: 982.6 bits (2539), Expect = 1.2e-282
Identity = 470/475 (98.95%), Postives = 472/475 (99.37%), Query Frame = 0


Query: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60
           MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP
Sbjct: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60

Query: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120
           PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG
Sbjct: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120

Query: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGL 180
           FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSL+GDGL
Sbjct: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLQGDGL 180

Query: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240
           IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD
Sbjct: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240

Query: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300
           NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI
Sbjct: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300

Query: 301 TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360
           TCGPGHGI   SLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH
Sbjct: 301 TCGPGHGI---SLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360

Query: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420
           MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN
Sbjct: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420

Query: 421 LTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           LTLADIELLPARGDIVS+PFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC
Sbjct: 421 LTLADIELLPARGDIVSNPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 472

[Close]

BLAST of MS018951 vs. NCBI nr
Match: XP_022935453.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 815.5 bits (2105), Expect = 2.5e-232
Identity = 386/478 (80.75%), Postives = 427/478 (89.33%), Query Frame = 0


Query: 7   SFCFCIRIFIFFCLLFSVHGR---HAKKHKHSHHHDHYSISQPPSPPPSPSSGL------ 66
           SF  CI + IFF   F VHGR   HAK HKHSHHH H++ISQPPSP PSPS  L      
Sbjct: 7   SFALCITLIIFF---FPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPSPPLSPSPEP 66

Query: 67  ASPPDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILV 126
           A+PPDD    SS N++ G FDVRH+GAIGDG  DDTEAFKMAWDTACQSD+DD  AVILV
Sbjct: 67  ANPPDDNDGGSSFNAT-GFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDD-YAVILV 126

Query: 127 PYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRG 186
           PYGFSF+IQSTIFTGPCK G+VF +DGT +APDGPDAWP+SYSAHQWLVFYRV NMSLRG
Sbjct: 127 PYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLRG 186

Query: 187 DGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHF 246
           +G+IDGRGQKWWDLPCKPH+GG G+T  G CDSPIAIRFFMSSNLTVEGLRI+DSPQFHF
Sbjct: 187 NGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF 246

Query: 247 RFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDI 306
           RFDNCRDVYIDSL ITAPA+SPNTDGIHIENTNGV+I NS+VANGDDCISIGSGSYNVDI
Sbjct: 247 RFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCISIGSGSYNVDI 306

Query: 307 KNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFN 366
           +NITCGPGHGISIGSLGNHNSRACVSN+TVRDS IKVSDNGVRIKTWQGGFGAVRGVSFN
Sbjct: 307 RNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAVRGVSFN 366

Query: 367 NIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMP 426
           NIHMDNVRNPIIIDQFYCLNKGCLNQ+SAV+VSDI+Y DIKG+YDIRSPP+HFGCSDSMP
Sbjct: 367 NIHMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRSPPMHFGCSDSMP 426

Query: 427 CTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           C NLTLA+IEL P++GDI  DPFCWNAYG+LQTLTIPP+ CLLE+++RS +++++DYC
Sbjct: 427 CVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC 479

[Close]

BLAST of MS018951 vs. NCBI nr
Match: XP_038905655.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 814.7 bits (2103), Expect = 4.2e-232
Identity = 392/478 (82.01%), Postives = 430/478 (89.96%), Query Frame = 0


Query: 6   SSFCFCIR-IFIFFCLLFSVHGR---HAKKHK-HSHHHDHYSISQPPSPPPSPSSGLASP 65
           SS  FCI  I  FFCLLF VHGR   HAKKHK HSHHH+H++ISQPPSPPP+      SP
Sbjct: 7   SSLAFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHNHFTISQPPSPPPT------SP 66

Query: 66  P--DDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSS-AVILV 125
           P  DD G SSS+NS+ GIFDVR+FGAIGDG  DDT AFKMAWDTACQ DDDD + AVIL+
Sbjct: 67  PDDDDNGGSSSINST-GIFDVRNFGAIGDGTADDTMAFKMAWDTACQRDDDDDNYAVILI 126

Query: 126 PYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRG 185
           PYGFSF+IQSTIFTGPCK GV F + GTL+APDGPDAWP+SYSAHQWLVFYRVYNMSLRG
Sbjct: 127 PYGFSFMIQSTIFTGPCKNGVEFQVGGTLMAPDGPDAWPKSYSAHQWLVFYRVYNMSLRG 186

Query: 186 DGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHF 245
           +GLIDGRGQKWWDLPCKPHKG  G+T +  CDSPIAIRFFMSSNLTVEGLRI+DSPQFHF
Sbjct: 187 NGLIDGRGQKWWDLPCKPHKGVKGRTMSEPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF 246

Query: 246 RFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDI 305
           RFDNC+DV ID + ITAPA SPNTDGIHIENTNGV+I+NS VANGDDCISIGSGSYNVDI
Sbjct: 247 RFDNCKDVIIDGIHITAPALSPNTDGIHIENTNGVQIFNSEVANGDDCISIGSGSYNVDI 306

Query: 306 KNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFN 365
           +NITCGPGHGISIGSLGNHNSRACVSN+TVRDS IKVSDNGVRIKTWQGG+GAVRGVSFN
Sbjct: 307 RNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFN 366

Query: 366 NIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMP 425
           NI+MDNVRNPIIIDQFYCLNKGCLNQ+SAV+VSDITY DIKGTYDIRSPP+HFGCSDSMP
Sbjct: 367 NINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDITYVDIKGTYDIRSPPMHFGCSDSMP 426

Query: 426 CTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           C NLTLA+IEL P++G+++ DPFCWNAYGNLQTLTIPPVACLLES++RS ++SEKDYC
Sbjct: 427 CMNLTLANIELFPSKGEVLLDPFCWNAYGNLQTLTIPPVACLLESSSRSFLESEKDYC 477

[Close]

BLAST of MS018951 vs. NCBI nr
Match: XP_031737994.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein Csa_000831 [Cucumis sativus])

HSP 1 Score: 810.1 bits (2091), Expect = 1.0e-230
Identity = 387/479 (80.79%), Postives = 428/479 (89.35%), Query Frame = 0


Query: 5   ISSFCFCIR-IFIFFCLLFSVHGR---HAKKHK-HSHHHDHYSISQPPSPPPSPSSGLAS 64
           ISSF F +  I  FFCLLF VHGR   HAKKHK HSHHHDH++ISQPPSP PS     + 
Sbjct: 8   ISSFAFSMTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSP 67

Query: 65  PPDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSS---AVIL 124
           P D+GG SSS  +S  IFDVRHFGAIGDG  DDT AFKMAWDTACQ +DDD++    VIL
Sbjct: 68  PEDNGGPSSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVIL 127

Query: 125 VPYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLR 184
           VPYGFSF+IQSTIFTGPCK GVVF +DGTL+APDGPDAWP+ YSAHQWLVFYRVYNMSL+
Sbjct: 128 VPYGFSFMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQ 187

Query: 185 GDGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFH 244
           G+GLIDGRGQKWWDLPCKPHK   GKT TG CDSPIAIRFFMSS L VEG+RI+DSPQFH
Sbjct: 188 GNGLIDGRGQKWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFH 247

Query: 245 FRFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVD 304
           FRFDNC+DVYIDSL IT+PA SPNTDGIHIENTNGV+I+NSIVANGDDC+SIGSGS+NV+
Sbjct: 248 FRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVE 307

Query: 305 IKNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSF 364
           I+NITCGPGHGISIGSLGNHNSRACVSN+TVRDS IKVSDNGVRIKTWQGGFG VRGVSF
Sbjct: 308 IRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSF 367

Query: 365 NNIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSM 424
           NNI+MDNVRNPIIIDQFYCLNKGCLNQ+SAV+VSDI+Y DIKGTYDIRSPP+HFGCSDSM
Sbjct: 368 NNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSM 427

Query: 425 PCTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           PC NLTLA+IEL P+RGDI+ DPFCWNAYGNLQTLTIPP++CLLE+++RS ++SEKDYC
Sbjct: 428 PCMNLTLANIELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 483

[Close]

BLAST of MS018951 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.7e-151
Identity = 249/458 (54.37%), Postives = 331/458 (72.27%), Query Frame = 0


Query: 14  IFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASPPD---DGGYSS-- 73
           I+I   +LF  H  +  + ++ +H   + ++ P  PPP P    A+PPD      Y S  
Sbjct: 10  IYIISTVLFG-HFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPD 69

Query: 74  ---SLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLI 133
                + SG +FDV  FGA+GDG  DDT AF+ AW  AC  +    S V+L P G  F I
Sbjct: 70  PAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVE----SGVVLAPEGGVFKI 129

Query: 134 QSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRG 193
            STIF+GPCK G+VF +DG L+ PDGP+ WP   + +QWLVFYR+   +  G G ++G G
Sbjct: 130 TSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNG 189

Query: 194 QKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDV 253
           QKWWDLPCKPH+G  G +++G C SP  IRFFMS+N+ V+GLRI++SPQFH +FD C+ V
Sbjct: 190 QKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGV 249

Query: 254 YIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPG 313
            I+ +QI++P  SPNTDGIH+ NT  V IYNS+V+NGDDCISIG+G  +VDI+ +TCGP 
Sbjct: 250 LINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPS 309

Query: 314 HGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVR 373
           HGISIGSLG HNS+ACVSN+TVR++ I+ SDNG+R+KTWQGG G+V  + F NI M+NV 
Sbjct: 310 HGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVL 369

Query: 374 NPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLAD 433
           N II+DQ+YC +K C N++SAV V D+ Y +IKGTYD+RSPP+HF CSD++ CTN+T+++
Sbjct: 370 NCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSE 429

Query: 434 IELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESA 464
           +ELLP  G++V DPFCWNAYG  +TLTIPP+ CLL+ +
Sbjct: 430 VELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGS 462

[Close]

BLAST of MS018951 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 7.5e-91
Identity = 173/402 (43.03%), Postives = 236/402 (58.71%), Query Frame = 0


Query: 76  IFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQSTIFTGPCK 135
           +  V +FGA G+G+ DDT+AF  AW TAC S        ILVP  ++ L++    +GPCK
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSS---KVKTRILVPENYTCLLRPIDLSGPCK 111

Query: 136 KGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCKP 195
             +   I GT++AP+ PD W    +  +WL F+ +  +++ G G ++G GQ+WW+  CK 
Sbjct: 112 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKH 171

Query: 196 HKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQITAP 255
           +     +       +P A+ F    N+ VE L + DS Q H    +CR V I  L++ AP
Sbjct: 172 NHSNPCR------GAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAP 231

Query: 256 AFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGN 315
           A SPNTDGIHI  + G+ I N+ V+ GDDCISI   S  + I NI CGPGHGISIGSLG 
Sbjct: 232 ATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGK 291

Query: 316 HNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQFYC 375
             S   V ++TV  + I  + NGVRIKTWQGG G V  + F NI M+NV NPIIIDQ+YC
Sbjct: 292 SKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYC 351

Query: 376 -LNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPARGD 435
              K C NQ+SA+ + +I++  ++GT       +   CSDS PC N+ L DI+L P+ GD
Sbjct: 352 DSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGD 411

Query: 436 IVSDPFCWNAYGNLQTLTIPPVACL------LESAARSTIQS 471
             ++ FCW AYG+      PP  CL      LE + +S I S
Sbjct: 412 GFTESFCWEAYGSSSGQVYPP-PCLSDDTSFLEQSVQSGITS 441

[Close]

BLAST of MS018951 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.8e-85
Identity = 169/400 (42.25%), Postives = 230/400 (57.50%), Query Frame = 0


Query: 76  IFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQSTIFTGPCK 135
           IF+V  +GA+GDG  D TEAF  AW  AC+      SA++LVP    F++ +  F GPC+
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKK----PSAMLLVPGNKKFVVNNLFFNGPCQ 117

Query: 136 KGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCKP 195
               F +DG + A   P +W  +     WL F ++   +L G G+IDG+G++WW   C  
Sbjct: 118 PHFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC-- 177

Query: 196 HKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQITAP 255
            K   G+      D P AI+F  S+ L ++GL++ +SP+FH  F NC  V I  + ITAP
Sbjct: 178 -KWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAP 237

Query: 256 AFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGN 315
             SPNTDGI I  +    +  + +  GDDC++IG+GS N+ I+++ CGPGHGISIGSLG 
Sbjct: 238 RDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGR 297

Query: 316 HNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQFYC 375
            NSRA VS V V  +    + NG+RIKTWQGG G    + + N+ M N  NPI+I+QFYC
Sbjct: 298 ENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYC 357

Query: 376 LN-KGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPARGD 435
            +   C NQ SAV + D+TY +I+GT    +  +   CSDSMPC ++ L+DI L    G 
Sbjct: 358 TSASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGK 417

Query: 436 IVSDPFCW--NAYGNLQTLTIPPVACLLESAARSTIQSEK 473
           I S   C   NA G      IP    L  SA R   +S K
Sbjct: 418 IAS---CLNDNANGYFSGHVIPACKNLSPSAKRKESKSHK 443

[Close]

BLAST of MS018951 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 4.9e-82
Identity = 156/361 (43.21%), Postives = 219/361 (60.66%), Query Frame = 0


Query: 69  SLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQST 128
           S + +  +F+V  +GA+GDG  D TEAF   W+ AC+     +SAV+LVP    F + + 
Sbjct: 51  SRHDAATVFNVEQYGAVGDGKHDSTEAFATTWNAACKK----ASAVLLVPANKKFFVNNL 110

Query: 129 IFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKW 188
           +F GPC+  + F +DGT+VA   P  W  S     WL F ++ + +L G G+IDG+GQ+W
Sbjct: 111 VFRGPCQPHLSFKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQW 170

Query: 189 WDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYID 248
           W   CK      G+T     + P AI+   S ++TV+ L + +SP+FH  F  C  V I 
Sbjct: 171 WAGQCKV---VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQ 230

Query: 249 SLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGHGI 308
            L+I AP  SPNTDGI I  +    I   ++  GDDCI+IG+GS N+ IK++ CGPGHGI
Sbjct: 231 GLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGI 290

Query: 309 SIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPI 368
           SIGSLG  NSRA VS+V V  +    + NG+RIKTWQGG G    +++ N+ M N  NPI
Sbjct: 291 SIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPI 350

Query: 369 IIDQFYCLN-KGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIE 428
           +I+QFYC +   C NQ SAV +  +TY +I GT    +  +   CSDS+PCT + L+++ 
Sbjct: 351 LINQFYCTSASACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVS 400

[Close]

BLAST of MS018951 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.4e-81
Identity = 154/373 (41.29%), Postives = 222/373 (59.52%), Query Frame = 0


Query: 76  IFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQSTIFTGPCK 135
           +F+V H+GA+GDG  D T+AF+  W+ AC    +  SAV LVP    F++ + +F GPC+
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAAC----NKLSAVFLVPANKKFVVNNLVFYGPCQ 118

Query: 136 KGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCKP 195
               F +DGT+ A   P  W  S     W+ F R+ + +L G G+IDG+G +WW   CK 
Sbjct: 119 PHFSFKVDGTIAAYPDPAKWKNS---KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT 178

Query: 196 HKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQITAP 255
             G T     GR   P AI+   S ++TV+ L + +SP+FH  F  C  V I  ++I AP
Sbjct: 179 INGRTVCNDKGR---PTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAP 238

Query: 256 AFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGHGISIGSLGN 315
             SPNTDGI I  +   +I    +  GDDC+++G+GS N+ IK++TCGPGHG+SIGSLG 
Sbjct: 239 RDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGK 298

Query: 316 HNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQFYC 375
            NSR+ VS V +  +    + NG+RIKTWQGG G    +++ N+ M N  NPI+I+QFYC
Sbjct: 299 GNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYC 358

Query: 376 LN-KGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPARGD 435
            +   C NQ SAV + D+T+ +I GT    +  +   CSDS+PC+N+ L+++ L    G 
Sbjct: 359 TSAAACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGK 417

Query: 436 IVSDPFCWNAYGN 448
           + +   C N   N
Sbjct: 419 VAT---CVNKNAN 417

[Close]

BLAST of MS018951 vs. ExPASy TrEMBL
Match: A0A6J1DR43 (polygalacturonase At1g48100-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022378 PE=3 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 4.2e-286
Identity = 473/475 (99.58%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60
           MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP
Sbjct: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60

Query: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120
           PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG
Sbjct: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120

Query: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGL 180
           FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSL+GDGL
Sbjct: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLQGDGL 180

Query: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240
           IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD
Sbjct: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240

Query: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300
           NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI
Sbjct: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300

Query: 301 TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360
           TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH
Sbjct: 301 TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360

Query: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420
           MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN
Sbjct: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420

Query: 421 LTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           LTLADIELLPARGDIVS+PFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC
Sbjct: 421 LTLADIELLPARGDIVSNPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 475

[Close]

BLAST of MS018951 vs. ExPASy TrEMBL
Match: A0A6J1DNT2 (polygalacturonase At1g48100-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022378 PE=3 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 5.7e-283
Identity = 470/475 (98.95%), Postives = 472/475 (99.37%), Query Frame = 0


Query: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60
           MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP
Sbjct: 1   MLCHISSFCFCIRIFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASP 60

Query: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120
           PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG
Sbjct: 61  PDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYG 120

Query: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGL 180
           FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSL+GDGL
Sbjct: 121 FSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLQGDGL 180

Query: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240
           IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD
Sbjct: 181 IDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFD 240

Query: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300
           NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI
Sbjct: 241 NCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNI 300

Query: 301 TCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360
           TCGPGHGI   SLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH
Sbjct: 301 TCGPGHGI---SLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIH 360

Query: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420
           MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN
Sbjct: 361 MDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTN 420

Query: 421 LTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           LTLADIELLPARGDIVS+PFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC
Sbjct: 421 LTLADIELLPARGDIVSNPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 472

[Close]

BLAST of MS018951 vs. ExPASy TrEMBL
Match: A0A6J1FAK7 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 386/478 (80.75%), Postives = 427/478 (89.33%), Query Frame = 0


Query: 7   SFCFCIRIFIFFCLLFSVHGR---HAKKHKHSHHHDHYSISQPPSPPPSPSSGL------ 66
           SF  CI + IFF   F VHGR   HAK HKHSHHH H++ISQPPSP PSPS  L      
Sbjct: 7   SFALCITLIIFF---FPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPSPPLSPSPEP 66

Query: 67  ASPPDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILV 126
           A+PPDD    SS N++ G FDVRH+GAIGDG  DDTEAFKMAWDTACQSD+DD  AVILV
Sbjct: 67  ANPPDDNDGGSSFNAT-GFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDD-YAVILV 126

Query: 127 PYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRG 186
           PYGFSF+IQSTIFTGPCK G+VF +DGT +APDGPDAWP+SYSAHQWLVFYRV NMSLRG
Sbjct: 127 PYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLRG 186

Query: 187 DGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHF 246
           +G+IDGRGQKWWDLPCKPH+GG G+T  G CDSPIAIRFFMSSNLTVEGLRI+DSPQFHF
Sbjct: 187 NGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHF 246

Query: 247 RFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDI 306
           RFDNCRDVYIDSL ITAPA+SPNTDGIHIENTNGV+I NS+VANGDDCISIGSGSYNVDI
Sbjct: 247 RFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCISIGSGSYNVDI 306

Query: 307 KNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFN 366
           +NITCGPGHGISIGSLGNHNSRACVSN+TVRDS IKVSDNGVRIKTWQGGFGAVRGVSFN
Sbjct: 307 RNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAVRGVSFN 366

Query: 367 NIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMP 426
           NIHMDNVRNPIIIDQFYCLNKGCLNQ+SAV+VSDI+Y DIKG+YDIRSPP+HFGCSDSMP
Sbjct: 367 NIHMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRSPPMHFGCSDSMP 426

Query: 427 CTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           C NLTLA+IEL P++GDI  DPFCWNAYG+LQTLTIPP+ CLLE+++RS +++++DYC
Sbjct: 427 CVNLTLANIELFPSKGDIFLDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC 479

[Close]

BLAST of MS018951 vs. ExPASy TrEMBL
Match: A0A0A0LEQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.5e-230
Identity = 382/469 (81.45%), Postives = 422/469 (89.98%), Query Frame = 0


Query: 14  IFIFFCLLFSVHGR---HAKKHK-HSHHHDHYSISQPPSPPPSPSSGLASPPDDGGYSSS 73
           I  FFCLLF VHGR   HAKKHK HSHHHDH++ISQPPSP PS     + P D+GG SSS
Sbjct: 4   IIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPAGSPPEDNGGPSSS 63

Query: 74  LNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSS---AVILVPYGFSFLIQ 133
             +S  IFDVRHFGAIGDG  DDT AFKMAWDTACQ +DDD++    VILVPYGFSF+IQ
Sbjct: 64  SINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFSFMIQ 123

Query: 134 STIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQ 193
           STIFTGPCK GVVF +DGTL+APDGPDAWP+ YSAHQWLVFYRVYNMSL+G+GLIDGRGQ
Sbjct: 124 STIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLIDGRGQ 183

Query: 194 KWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVY 253
           KWWDLPCKPHK   GKT TG CDSPIAIRFFMSS L VEG+RI+DSPQFHFRFDNC+DVY
Sbjct: 184 KWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNCKDVY 243

Query: 254 IDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGH 313
           IDSL IT+PA SPNTDGIHIENTNGV+I+NSIVANGDDC+SIGSGS+NV+I+NITCGPGH
Sbjct: 244 IDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITCGPGH 303

Query: 314 GISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRN 373
           GISIGSLGNHNSRACVSN+TVRDS IKVSDNGVRIKTWQGGFG VRGVSFNNI+MDNVRN
Sbjct: 304 GISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMDNVRN 363

Query: 374 PIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADI 433
           PIIIDQFYCLNKGCLNQ+SAV+VSDI+Y DIKGTYDIRSPP+HFGCSDSMPC NLTLA+I
Sbjct: 364 PIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSPPMHFGCSDSMPCMNLTLANI 423

Query: 434 ELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           EL P+RGDI+ DPFCWNAYGNLQTLTIPP++CLLE+++RS ++SEKDYC
Sbjct: 424 ELFPSRGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRSFLESEKDYC 469

[Close]

BLAST of MS018951 vs. ExPASy TrEMBL
Match: A0A5D3DA93 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00750 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 392/489 (80.16%), Postives = 433/489 (88.55%), Query Frame = 0


Query: 5   ISSFCFCIR-IFIFFCLLFSVHGR---HAKKHK-HSHHHDHYSISQP----------PSP 64
           ISSF FCI  I  FFCLLF V+GR   HAKKHK HSHHH H++ISQP          PSP
Sbjct: 8   ISSFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSP 67

Query: 65  PPSPSSGLASPP-DDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDD 124
            PSPSS   SPP DDGG +SS+NS+  IFDVRHFGAIGDG  DDT AFKMAWDTACQ +D
Sbjct: 68  SPSPSSEPQSPPEDDGGPASSINST-IIFDVRHFGAIGDGTADDTAAFKMAWDTACQRND 127

Query: 125 DDSS--AVILVPYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLV 184
           DD +   VILVPYGFSF+IQSTIFTGPC+ G+VF +DGTL+APDGPDAWP+ YSAHQWLV
Sbjct: 128 DDDNNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLV 187

Query: 185 FYRVYNMSLRGDGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEG 244
           FYRVYNMSL+G+GLIDGRGQKWWDLPCKPHK   GKT TG CDSPIAIRFFMSS L VEG
Sbjct: 188 FYRVYNMSLQGNGLIDGRGQKWWDLPCKPHK---GKTMTGPCDSPIAIRFFMSSKLRVEG 247

Query: 245 LRIRDSPQFHFRFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCI 304
           +RI+DSPQFHFRFDNC+DVYIDSL IT+PA SPNTDGIHIENTNGV+I+NSIVANGDDCI
Sbjct: 248 VRIKDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCI 307

Query: 305 SIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQG 364
           SIGSGSYNV+I NITCGPGHGISIGSLGNHNSRACVSN+TVRDS IKVSDNGVRIKTWQG
Sbjct: 308 SIGSGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQG 367

Query: 365 GFGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSP 424
           G+GAVRGVSFNNI+MDNVRNPIIIDQFYCLNKGCLNQ+SAV+VSDI+Y DIKGTYDIRSP
Sbjct: 368 GYGAVRGVSFNNINMDNVRNPIIIDQFYCLNKGCLNQTSAVVVSDISYIDIKGTYDIRSP 427

Query: 425 PLHFGCSDSMPCTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESAARS 476
           P+HFGCSDSMPC NLTLA+IEL P++GDI+ DPFCWNAYGNLQTLTIPP++CLLE+++RS
Sbjct: 428 PMHFGCSDSMPCMNLTLANIELFPSKGDILLDPFCWNAYGNLQTLTIPPISCLLETSSRS 487

[Close]

BLAST of MS018951 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.7e-183
Identity = 300/463 (64.79%), Postives = 369/463 (79.70%), Query Frame = 0


Query: 28  HAKKHKHSHHHDH--------YSISQPPSPPPSPSSGLA-----SPPDDGGYSSSLNSSG 87
           H +KHKH++H+ H         SISQPP+PPP P    A     SP DD       + + 
Sbjct: 32  HKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSLPPSPSDD-----PADDNN 91

Query: 88  GIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQSTIFTGPC 147
           GI++VR +GA+GDG  DDTEAFK AWD++C ++++++ +V+LVPYG++F+IQSTIFTGPC
Sbjct: 92  GIYNVRKYGAVGDGETDDTEAFKTAWDSSC-NNENNTDSVLLVPYGYTFMIQSTIFTGPC 151

Query: 148 KKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCK 207
           +    F +DGT+V PDGP++WP + S  QWLVFYRV  M+L+G+G+IDGRGQKWWDLPCK
Sbjct: 152 RSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVIDGRGQKWWDLPCK 211

Query: 208 PHKG-GTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQIT 267
           PH+        TG CDSPIA+RFFMSSNL VEGL+I++SPQFHFRFD C+ V+++SL IT
Sbjct: 212 PHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDGCQGVHVESLHIT 271

Query: 268 APAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGP-GHGISIGS 327
           AP  SPNTDGIHIEN+N V IYNSI++NGDDC+SIGSGSY+VDI+N+TCGP GHGISIGS
Sbjct: 272 APPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLTCGPGGHGISIGS 331

Query: 328 LGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQ 387
           LGNHNSRACVSN+TVRDS IK SDNGVRIKTWQGG G+V GV+FNNIH+D+VRNPIIIDQ
Sbjct: 332 LGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIHVDSVRNPIIIDQ 391

Query: 388 FYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPAR 447
           +YC+ K C N++SAV VSDI Y  IKGTYDIRSPP+HFGCSD++PCTNLTL+DIELLPA+
Sbjct: 392 YYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTNLTLSDIELLPAK 451

Query: 448 GDIVSDPFCWNAYGNLQTLTIPPVACLLESAARSTIQSEKDYC 476
           G+IV DPFCWNAYG  + L+IPPV CL+    +    S  D C
Sbjct: 452 GEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQGSLVDKC 488

[Close]

BLAST of MS018951 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.1e-160
Identity = 256/438 (58.45%), Postives = 334/438 (76.26%), Query Frame = 0


Query: 40  HYSISQPPSPPPSPSSGLASPPDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMA 99
           H+  SQPPSP P+P+    SP         +     ++DVR +GA+G+G+ DDT +FK A
Sbjct: 33  HHQASQPPSPSPNPNDPSKSPSRSQDLDHEV-----VYDVRKYGAVGNGVADDTVSFKTA 92

Query: 100 WDTACQSDDDDSSAVILVPYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSY 159
           WD+AC ++ +++++V+ VPYGF+F+I+STIFTGPC+    F +DGT+V  DGP +WP S 
Sbjct: 93  WDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSL 152

Query: 160 SAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCKPHKGGTGKT-TTGRCDSPIAIRFFM 219
           +  QWL FYR+  M+L+G G+IDGRGQ WWDLPCKPH+    KT   G C+SP A+RFFM
Sbjct: 153 NKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFM 212

Query: 220 SSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSI 279
           SSN+ V+GL I++SPQ H + D C  V+I+SL+I +P  SPNTDGIHIEN+N V+IYNS+
Sbjct: 213 SSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSV 272

Query: 280 VANGDDCISIGSGSYNVDIKNITCGP-GHGISIGSLGNHNSRACVSNVTVRDSTIKVSDN 339
           ++NGDDC+SIG G+Y++DI+NITCGP GHGISIGSLG  NS ACVSNVTVRDS IK S+N
Sbjct: 273 ISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSEN 332

Query: 340 GVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDI 399
           GVRIKTWQGG G+V GV+F+NIH+D VRNPIIIDQ+YC  K C N++SAV V+DI Y  I
Sbjct: 333 GVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSI 392

Query: 400 KGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVA 459
           KGTYDIRSPP+HFGCS+++PCTNLTL++IELLP++ DIV  PFCWNAYG     ++P ++
Sbjct: 393 KGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLIS 452

Query: 460 CLLESAARSTIQSEKDYC 476
           CL  + + S +      C
Sbjct: 453 CLKSNPSTSLLSGLSGRC 465

[Close]

BLAST of MS018951 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 545.8 bits (1405), Expect = 3.4e-155
Identity = 252/421 (59.86%), Postives = 322/421 (76.48%), Query Frame = 0


Query: 42  SISQPPSPPPSPSSGLASPPDDGGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWD 101
           SI   PSP P+P+           Y+ + N +  +FDV  FGAIGD   DDT AFKMAWD
Sbjct: 24  SIYLSPSPAPNPA-----------YNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWD 83

Query: 102 TACQSDDDDSSAVILVPYGFSFLIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSA 161
            AC S     SA++LVPY F FL++ T F GPC+  +V  IDG +V+PDGP +WP +Y  
Sbjct: 84  AACMS-TGPKSALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQ- 143

Query: 162 HQWLVFYRVYNMSLRGDGLIDGRGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSN 221
            QW++FYRV  +S++G G+I+GRGQKWW+LPCKPHKG  G T TG CDSP+AIR F SS 
Sbjct: 144 RQWMMFYRVNGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSK 203

Query: 222 LTVEGLRIRDSPQFHFRFDNCRDVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVAN 281
           + ++G+   +S QFH RFDNC DV +DS+ I APA SPNTDGIHIENT+ V+I NS+++N
Sbjct: 204 VRIQGINFMNSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISN 263

Query: 282 GDDCISIGSGSYNVDIKNITCGPGHGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRI 341
           GDDCISIG+G +NVDIKN+TCGP HGISIGSLG HNS+A VSN+TV +STI  SDNGVRI
Sbjct: 264 GDDCISIGAGCFNVDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRI 323

Query: 342 KTWQGGFGAVRGVSFNNIHMDNVRNPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTY 401
           KTWQGG G+V  + F+NI M NVRNPI+IDQ+YC    C NQ+SAV++SD+ Y +IKGTY
Sbjct: 324 KTWQGGSGSVSRIVFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTY 383

Query: 402 DIRSPPLHFGCSDSMPCTNLTLADIELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLE 461
           D+RSPP+HFGCSDS+PCTNLTL +++L P++G  + +PFCWNAYG+++ +T+PPV CLL+
Sbjct: 384 DLRSPPIHFGCSDSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLD 430

Query: 462 S 463
           +
Sbjct: 444 A 430

[Close]

BLAST of MS018951 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-152
Identity = 249/458 (54.37%), Postives = 331/458 (72.27%), Query Frame = 0


Query: 14  IFIFFCLLFSVHGRHAKKHKHSHHHDHYSISQPPSPPPSPSSGLASPPD---DGGYSS-- 73
           I+I   +LF  H  +  + ++ +H   + ++ P  PPP P    A+PPD      Y S  
Sbjct: 10  IYIISTVLFG-HFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPD 69

Query: 74  ---SLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLI 133
                + SG +FDV  FGA+GDG  DDT AF+ AW  AC  +    S V+L P G  F I
Sbjct: 70  PAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVE----SGVVLAPEGGVFKI 129

Query: 134 QSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRG 193
            STIF+GPCK G+VF +DG L+ PDGP+ WP   + +QWLVFYR+   +  G G ++G G
Sbjct: 130 TSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNG 189

Query: 194 QKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDV 253
           QKWWDLPCKPH+G  G +++G C SP  IRFFMS+N+ V+GLRI++SPQFH +FD C+ V
Sbjct: 190 QKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGV 249

Query: 254 YIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPG 313
            I+ +QI++P  SPNTDGIH+ NT  V IYNS+V+NGDDCISIG+G  +VDI+ +TCGP 
Sbjct: 250 LINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPS 309

Query: 314 HGISIGSLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVR 373
           HGISIGSLG HNS+ACVSN+TVR++ I+ SDNG+R+KTWQGG G+V  + F NI M+NV 
Sbjct: 310 HGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVL 369

Query: 374 NPIIIDQFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLAD 433
           N II+DQ+YC +K C N++SAV V D+ Y +IKGTYD+RSPP+HF CSD++ CTN+T+++
Sbjct: 370 NCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSE 429

Query: 434 IELLPARGDIVSDPFCWNAYGNLQTLTIPPVACLLESA 464
           +ELLP  G++V DPFCWNAYG  +TLTIPP+ CLL+ +
Sbjct: 430 VELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGS 462

[Close]

BLAST of MS018951 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-106
Identity = 190/406 (46.80%), Postives = 262/406 (64.53%), Query Frame = 0


Query: 73  SGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSFLIQSTIFTG 132
           S G  +V   GA GDG  DDT+AF+ AW  AC+     +++ +LVP G +FL+    F G
Sbjct: 47  SSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKV----AASTLLVPSGSTFLVGPVSFLG 106

Query: 133 -PCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLRGDGLIDGRGQKWWDL 192
             CK+ +VF ++G ++AP    AW       QW+ F  +  ++++G G+IDGRG  WW+ 
Sbjct: 107 KECKEKIVFQLEGKIIAPTSASAW--GSGLLQWIEFKALQGITIKGKGIIDGRGSVWWN- 166

Query: 193 PCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCRDVYIDSLQ 252
                  GT    T     P A+RF+ S+ +TV G+ I++SPQ H +FDNC  + +    
Sbjct: 167 ----DMMGTKMPRT----KPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFT 226

Query: 253 ITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCGPGHGISIG 312
            ++P  SPNTDGIH++N+    IY S +A GDDCISI +G  N++I ++ CGPGHGISIG
Sbjct: 227 TSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIG 286

Query: 313 SLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPIIID 372
            LG  N++ACVSN+TVRD T+  + NGVRIK+WQGG G+V+ V F+NI + NV NPIIID
Sbjct: 287 GLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIID 346

Query: 373 QFYCLNKGCLNQSSAVLVSDITYTDIKGTYDIRSPPLHFGCSDSMPCTNLTLADIELLPA 432
           Q+YC   GC N++SAV VS+I Y +IKGTY     P+ F CSDS+PCT ++L+ IEL PA
Sbjct: 347 QYYCDGGGCHNETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTIELKPA 406

Query: 433 RGDIVS-DPFCWNAYGNLQTLTIPPVACL-LESAARSTIQSEKDYC 476
            G   S DPFCW A+G L+T T+PP+ CL  E +  +  +S  D C
Sbjct: 407 TGKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

The following BLAST results are available for this feature:

BLAST of MS018951 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155236.1	8.6e-286	99.58	polygalacturonase At1g48100-like isoform X1 [Momordica charantia]	[more]
XP_022155237.1	1.2e-282	98.95	polygalacturonase At1g48100-like isoform X2 [Momordica charantia]	[more]
XP_022935453.1	2.5e-232	80.75	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_038905655.1	4.2e-232	82.01	polygalacturonase At1g48100-like [Benincasa hispida]	[more]
XP_031737994.1	1.0e-230	80.79	polygalacturonase At1g48100 [Cucumis sativus] >KGN58717.2 hypothetical protein C...	[more]

BLAST of MS018951 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.7e-151	54.37	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	7.5e-91	43.03	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	2.8e-85	42.25	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	4.9e-82	43.21	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9FY19	2.4e-81	41.29	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]

BLAST of MS018951 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DR43	4.2e-286	99.58	polygalacturonase At1g48100-like isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1DNT2	5.7e-283	98.95	polygalacturonase At1g48100-like isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1FAK7	1.2e-232	80.75	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 P...	[more]
A0A0A0LEQ6	2.5e-230	81.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1	[more]
A0A5D3DA93	1.2e-229	80.16	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00...	[more]

BLAST of MS018951 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02460.1	1.7e-183	64.79	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	2.1e-160	58.45	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	3.4e-155	59.86	Pectin lyase-like superfamily protein	[more]
AT1G48100.1	1.2e-152	54.37	Pectin lyase-like superfamily protein	[more]
AT5G14650.1	2.2e-106	46.80	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 322..343 e-value: 1.9 score: 17.5 coord: 219..268 e-value: 570.0 score: 9.0 coord: 292..312 e-value: 3500.0 score: 3.3 coord: 351..372 e-value: 2900.0 score: 3.9 coord: 384..405 e-value: 10000.0 score: 0.1 coord: 269..290 e-value: 320.0 score: 10.1
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 114..428 e-value: 2.5E-90 score: 302.9
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 299..312
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 65..472 e-value: 3.6E-118 score: 397.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..67
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 10..465
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 10..465
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 75..460

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018951.1	MS018951.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity