MS018818 (gene) Bitter gourd (TR) v1

BLAST of MS018818 vs. NCBI nr
Match: XP_022137718.1 (putative pectate lyase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 722.6 bits (1864), Expect = 1.8e-204
Identity = 349/358 (97.49%), Postives = 351/358 (98.04%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 92  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 151
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 152 HGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 211
           HGLIIHHCRAQAAAQVMGP+SKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 212 RGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAH 271
           RGSTDITISNNWFRDQDKVILLGHDDGYVRD RMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 272 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDW---VVWSTGNVEETKWNFH 331
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE   +W   VVWSTGNVEETKWNFH
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEKILNWRLQVVWSTGNVEETKWNFH 300

Query: 332 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC
Sbjct: 301 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 358

BLAST of MS018818 vs. NCBI nr
Match: XP_022137719.1 (putative pectate lyase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 719.2 bits (1855), Expect = 2.0e-203
Identity = 348/355 (98.03%), Postives = 349/355 (98.31%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 92  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 151
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 152 HGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 211
           HGLIIHHCRAQAAAQVMGP+SKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 212 RGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAH 271
           RGSTDITISNNWFRDQDKVILLGHDDGYVRD RMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 272 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 331
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE     VVWSTGNVEETKWNFHSVR
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE-----VVWSTGNVEETKWNFHSVR 300

Query: 332 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC
Sbjct: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 350

BLAST of MS018818 vs. NCBI nr
Match: XP_022923821.1 (putative pectate lyase 2 [Cucurbita moschata])

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-188
Identity = 322/382 (84.29%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 6   ASQLFFLSLISFALLSAAACS-ARGLNMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 65
           A+QLF LSLIS  LLSA + S   GL MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 66  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISSFT 125
           NNIGRNLIRYQVTDPSDDPINPRPGTLRYGA++I+ KVWITF++DM I LHKPL+ISS+T
Sbjct: 63  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 126 AIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDA 185
           AIDGRGA+VHI+GNACL+V +A+NIIIHGLIIHHC+AQ A QVMGP+SK++SLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDWR 245
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD  
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK 305
           M+VTVVYNHFGPNCNQRMPRIR+GYAHVANN+YQGWTQYAIGGSMNPSVKSEANLFIASK
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 306 SKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVAD 365
           SK+     +VWSTG VE  KW FHSVRDVFENGASF QIGVGRG V PNY+AQQRF VAD
Sbjct: 303 SKQ-----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVAD 362

Query: 366 AKWVRSLTTSSGALRCARNSRC 387
           AKWVRSLT+SSGALRC+  SRC
Sbjct: 363 AKWVRSLTSSSGALRCSSKSRC 379

BLAST of MS018818 vs. NCBI nr
Match: KAG7020030.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.8 bits (1727), Expect = 1.4e-188
Identity = 321/382 (84.03%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 6   ASQLFFLSLISFALLSAAACS-ARGLNMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 65
           A+QLF LSLIS  LLSA + S   GL MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 66  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISSFT 125
           NN+GRNLIRYQVTDPSDDPINPRPGTLRYGA++I+ KVWITF++DM I LHKPL+ISS+T
Sbjct: 63  NNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 126 AIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDA 185
           AIDGRGA+VHI+GNACL+V +A+NIIIHGLIIHHC+AQ A QVMGP+SK++SLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDWR 245
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD  
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK 305
           M+VTVVYNHFGPNCNQRMPRIR+GYAHVANN+YQGWTQYAIGGSMNPSVKSEANLFIASK
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 306 SKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVAD 365
           SK+     +VWSTG VE  KW FHSVRDVFENGASF QIGVGRG V PNY+AQQRF VAD
Sbjct: 303 SKQ-----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVAD 362

Query: 366 AKWVRSLTTSSGALRCARNSRC 387
           AKWVRSLT+SSGALRC+  SRC
Sbjct: 363 AKWVRSLTSSSGALRCSSKSRC 379

BLAST of MS018818 vs. NCBI nr
Match: XP_023520094.1 (putative pectate lyase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669.5 bits (1726), Expect = 1.8e-188
Identity = 320/382 (83.77%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 6   ASQLFFLSLISFALLSAAACS-ARGLNMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 65
           A+QLF LSLIS  LLSA + S   GL MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 66  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISSFT 125
           NN+GRNLIRYQVTDPSDDPINPRPGTLRYGA++++ KVWITF++DM I LHKPL+ISS+T
Sbjct: 63  NNVGRNLIRYQVTDPSDDPINPRPGTLRYGATMMKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 126 AIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDA 185
           AIDGRGA+VHI+GNACL+V +A+NIIIHGLIIHHC+AQ A QVMGP+SK++SLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDWR 245
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD  
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDMN 242

Query: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK 305
           M+VTVVYNHFGPNCNQRMPRIR+GYAHVANN+YQGWTQYAIGGSMNPSVKSEANLFIASK
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 306 SKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVAD 365
           SK+     +VWSTG VE  KW FHSVRDVFENGASF QIGVGRG V PNY+AQQRF VAD
Sbjct: 303 SKQ-----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVAD 362

Query: 366 AKWVRSLTTSSGALRCARNSRC 387
           AKWVRSLT+SSGALRC+  SRC
Sbjct: 363 AKWVRSLTSSSGALRCSSKSRC 379

BLAST of MS018818 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 322.8 bits (826), Expect = 5.4e-87
Identity = 180/396 (45.45%), Postives = 236/396 (59.60%), Query Frame = 0

Query: 11  FLSLIS--FALLSAAACSARGLN--------MNAIDRCWRSNPYWRRNRQQLGVCSVGFV 70
           FL++IS  FA  S++   A   N         N ID CWR NPYW  NR+ L  C+VGF 
Sbjct: 5   FLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF- 64

Query: 71  GKMTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGK-VWITFRRDMRITLHKPLM 130
           GK     G+    Y VT+PSDDP NPRPGTLRY  +VI+ K +WITF RDM I L   L+
Sbjct: 65  GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLRY--AVIQSKPLWITFARDMVIVLRNELI 124

Query: 131 ISSFTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGH 190
           ++S+  IDGRGA V IA   C+ +   S++IIHG+ IH C+   + +V    + + S   
Sbjct: 125 MNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKG 184

Query: 191 VDGDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGY 250
            DGDAI +  +S +WIDH    RC+DGL+DV   ST +TISNN+F   DKV+LLGH+D  
Sbjct: 185 SDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNN 244

Query: 251 VRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANL 310
           V D  M+VT+ +NHFGP   +RMPR+R GYAHVANN Y+ W  YAIGGS +P++ SE N 
Sbjct: 245 VEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNY 304

Query: 311 FIAS---------KSKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVV 370
           F+AS         K  ++ YDW           +W + + +DVF+NGA F  +  G G V
Sbjct: 305 FVASDDPSKKQVTKRIDSGYDW----------KRWKWRTSKDVFKNGAYF--VPSGYGTV 364

Query: 371 TPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           TP Y   +RF V+    V  LT+S+G L C     C
Sbjct: 365 TPLYGRAERFPVSHGSLVPLLTSSAGPLHCYSGRIC 384

BLAST of MS018818 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.2e-84
Identity = 175/383 (45.69%), Postives = 225/383 (58.75%), Query Frame = 0

Query: 5   MASQLFFLSLISFALLSAAACSARGLN-------MNAIDRCWRSNPYWRRNRQQLGVCSV 64
           MAS +  +SL+  A  S    +A   N       +N ID CWR NP W  NRQ L  C+V
Sbjct: 1   MASLVVIVSLLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAV 60

Query: 65  GFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKP 124
           G+ GK     G+N   Y VT+PSD+P  P PGTLRY  S  +  +WITF RDM I L   
Sbjct: 61  GY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPK-PLWITFARDMVIVLKSQ 120

Query: 125 LMISSFTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISL 184
           LMI+S+  IDGRGA V IA   CL + +  ++IIHG+ IH C+A                
Sbjct: 121 LMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADP-------------- 180

Query: 185 GHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDD 244
             +DGD IR+  ++ VWIDH  L RC DGL+DV   ST +TISNN+F   DKV+LLGHDD
Sbjct: 181 NGMDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDD 240

Query: 245 GYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEA 304
            Y+ D  M+VT+ +N FGP   +RMPR+R GYAHVANN Y+ W  YAIGGS NP + SE 
Sbjct: 241 SYMGDKDMRVTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEG 300

Query: 305 NLFIASKSKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQ 364
           N F+A + + ++        G  +  +W + + RDVF NGA F   GV   +V P Y   
Sbjct: 301 NYFVAPEKRSSKQVTKRMMAG-PDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGG 360

Query: 365 QRFQVADAKWVRSLTTSSGALRC 381
           + F+VA    V SLT+S+G LRC
Sbjct: 361 EGFRVAHGSLVPSLTSSAGPLRC 362

BLAST of MS018818 vs. ExPASy Swiss-Prot
Match: O65456 (Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.4e-84
Identity = 168/359 (46.80%), Postives = 215/359 (59.89%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MN +D CWR    W  NR+ L  C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 92  RYGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNII 151
           RYG  VI+ K +WITF +DM ITL   LM++S+  IDGRGA V IA   C+ +   +N+I
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 152 IHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDV 211
           +HG+ IH C+      V    + +      DGDAI +  +S +WIDH  L  C DGL+DV
Sbjct: 166 VHGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDV 225

Query: 212 TRGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYA 271
              ST ITISNN+F   DKV+LLGH+D +V+D +MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 272 HVANNMYQGWTQYAIGGSMNPSVKSEANLFIA---SKSKEARYDWVVWSTGNVEETKWNF 331
           HVANN Y  W  YAIGGS +P++ SE N FIA   S SKE     V     N     W +
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNN-----WRW 345

Query: 332 HSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
            + +DVF+NGA F  +  G G ++  Y++ QRF VA    V SLT  +G L C RN  C
Sbjct: 346 RTSKDVFKNGAYF--VPSGYGSISLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

BLAST of MS018818 vs. ExPASy Swiss-Prot
Match: O65457 (Putative pectate lyase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g22090 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.1e-83
Identity = 168/359 (46.80%), Postives = 215/359 (59.89%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MN +D CWR    W  NR+ L  C+VGF          N+  Y VT+P D+  NP PG+L
Sbjct: 46  MNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTNPYDNAQNPHPGSL 105

Query: 92  RYGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNII 151
           RYG  VI+ K +WITF +DM ITL   LM++S+  IDGRGA V IA   C+ +   +N+I
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 152 IHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDV 211
           +HG+ IH C+   + +V    + +      DGDAI +  +S VWIDH  L  C DGL+DV
Sbjct: 166 VHGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDV 225

Query: 212 TRGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYA 271
              ST ITISNN+F   DKV+LLGH+D +V+D +MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 272 HVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK---SKEARYDWVVWSTGNVEETKWNF 331
           HVANN Y  W  YAIGGS +P++ SE N FIAS    SKE     V     N     W +
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSYSKEVTKREVKGGWNN-----WRW 345

Query: 332 HSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
            +  DVF+NGA F  +  G G +   Y++ QRF VA    V SLT  +G L C RN  C
Sbjct: 346 RTSNDVFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

BLAST of MS018818 vs. ExPASy Swiss-Prot
Match: Q9SVQ6 (Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2)

HSP 1 Score: 308.1 bits (788), Expect = 1.4e-82
Identity = 163/355 (45.92%), Postives = 207/355 (58.31%), Query Frame = 0

Query: 33  NAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSD-DPINPRPGTL 92
           N ID CWR +  W R R++L  C++GF        GR+   Y VTDPSD DP+ P+PGTL
Sbjct: 90  NPIDDCWRCDKKWHRRRKRLADCAIGFGRNAVG--GRDGRYYIVTDPSDHDPVTPKPGTL 149

Query: 93  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 152
           RY A +    +WI F+RDM ITL + L+++SF  IDGRG  VHIAG ACL V   +NIII
Sbjct: 150 RY-AVIQDEPLWIVFKRDMVITLSQELIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIII 209

Query: 153 HGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 212
           HG+ IH C+    A V   +S        DGD I +  +S +WIDHN+L  C DGL+D  
Sbjct: 210 HGINIHDCKRTGNAMVRSSESHYGWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAI 269

Query: 213 RGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAH 272
            GST ITISNN+    ++ ILLGH D Y RD  M+VT+ YNHFG    QRMPR R+GY H
Sbjct: 270 MGSTAITISNNYLTHHNEAILLGHTDSYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFH 329

Query: 273 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 332
           V NN Y  W  YAIGGS NP++ S+ N F+A  ++ A+        G  E   WN+ S  
Sbjct: 330 VVNNDYTHWEMYAIGGSANPTINSQGNRFLAPGNRFAKEVTKRVGAGKGEWNNWNWRSQG 389

Query: 333 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           D+  NGA F   G G    + NY          +  V  LT+SSGAL+C   + C
Sbjct: 390 DLMLNGAYFTSSGAG---ASANYARASSLAAKSSSLVGMLTSSSGALKCRIGTLC 438

BLAST of MS018818 vs. ExPASy TrEMBL
Match: A0A6J1CB55 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 8.6e-205
Identity = 349/358 (97.49%), Postives = 351/358 (98.04%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 92  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 151
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 152 HGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 211
           HGLIIHHCRAQAAAQVMGP+SKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 212 RGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAH 271
           RGSTDITISNNWFRDQDKVILLGHDDGYVRD RMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 272 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDW---VVWSTGNVEETKWNFH 331
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE   +W   VVWSTGNVEETKWNFH
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEKILNWRLQVVWSTGNVEETKWNFH 300

Query: 332 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC
Sbjct: 301 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 358

BLAST of MS018818 vs. ExPASy TrEMBL
Match: A0A6J1C826 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 9.5e-204
Identity = 348/355 (98.03%), Postives = 349/355 (98.31%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 92  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 151
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 152 HGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 211
           HGLIIHHCRAQAAAQVMGP+SKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 212 RGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAH 271
           RGSTDITISNNWFRDQDKVILLGHDDGYVRD RMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 272 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 331
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE     VVWSTGNVEETKWNFHSVR
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE-----VVWSTGNVEETKWNFHSVR 300

Query: 332 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC
Sbjct: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 350

BLAST of MS018818 vs. ExPASy TrEMBL
Match: A0A6J1E7R9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.0e-189
Identity = 322/382 (84.29%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 6   ASQLFFLSLISFALLSAAACS-ARGLNMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 65
           A+QLF LSLIS  LLSA + S   GL MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 66  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISSFT 125
           NNIGRNLIRYQVTDPSDDPINPRPGTLRYGA++I+ KVWITF++DM I LHKPL+ISS+T
Sbjct: 63  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKDMHILLHKPLLISSYT 122

Query: 126 AIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDA 185
           AIDGRGA+VHI+GNACL+V +A+NIIIHGLIIHHC+AQ A QVMGP+SK++SLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDWR 245
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD  
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK 305
           M+VTVVYNHFGPNCNQRMPRIR+GYAHVANN+YQGWTQYAIGGSMNPSVKSEANLFIASK
Sbjct: 243 MRVTVVYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 306 SKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVAD 365
           SK+     +VWSTG VE  KW FHSVRDVFENGASF QIGVGRG V PNY+AQQRF VAD
Sbjct: 303 SKQ-----IVWSTGKVEAAKWKFHSVRDVFENGASFGQIGVGRGGVKPNYSAQQRFPVAD 362

Query: 366 AKWVRSLTTSSGALRCARNSRC 387
           AKWVRSLT+SSGALRC+  SRC
Sbjct: 363 AKWVRSLTSSSGALRCSSKSRC 379

BLAST of MS018818 vs. ExPASy TrEMBL
Match: A0A1S3AY18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.5e-188
Identity = 320/385 (83.12%), Postives = 345/385 (89.61%), Query Frame = 0

Query: 5   MASQLFFLSLISFALLSA---AACSARGLNMNAIDRCWRSNPYWRRNRQQLGVCSVGFVG 64
           +  QL  LSLISF  L +   ++  A GL+MNAIDRCWRSNPYWRRNRQQL  CSVGF G
Sbjct: 3   ITQQLSVLSLISFIFLISNVVSSSEAHGLSMNAIDRCWRSNPYWRRNRQQLATCSVGFAG 62

Query: 65  KMTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMIS 124
           KMTNN+GRNLI YQVTDPSDDPINPRPGTLRYGA++I+ KVWITF++DM I L KPL+IS
Sbjct: 63  KMTNNMGRNLIHYQVTDPSDDPINPRPGTLRYGATMIKHKVWITFQKDMHIVLQKPLLIS 122

Query: 125 SFTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVD 184
           SFTAIDGRGATVHI GNACLMVLRA+NIIIHGLIIHHCR+QAA +VMGP+SK+ISLGHVD
Sbjct: 123 SFTAIDGRGATVHITGNACLMVLRATNIIIHGLIIHHCRSQAAGEVMGPNSKVISLGHVD 182

Query: 185 GDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVR 244
           GDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVR
Sbjct: 183 GDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVR 242

Query: 245 DWRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFI 304
           D  MKVTVVYNHFGPNCNQRMPRIRYGYAHVANN+YQGWTQYAIGGSMNPSVKSEANLFI
Sbjct: 243 DRNMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFI 302

Query: 305 ASKSKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQ 364
           ASKSK+     V+WSTGNVEE KW FHSVRDVFENGA F +IGVGRG V PNYN QQRF 
Sbjct: 303 ASKSKQ-----VIWSTGNVEEAKWKFHSVRDVFENGALFTEIGVGRGRVKPNYNPQQRFP 362

Query: 365 VADAKWVRSLTTSSGALRCARNSRC 387
           VADAKW+R+LT+SSGALRC  NS+C
Sbjct: 363 VADAKWIRALTSSSGALRCTSNSKC 382

BLAST of MS018818 vs. ExPASy TrEMBL
Match: A0A6J1KLU6 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111495358 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.8e-186
Identity = 318/382 (83.25%), Postives = 347/382 (90.84%), Query Frame = 0

Query: 6   ASQLFFLSLISFALLSAAACS-ARGLNMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 65
           A+QLF LSLIS  LLSA + S   GL MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT
Sbjct: 3   ATQLFVLSLISLTLLSAVSYSEGNGLKMNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMT 62

Query: 66  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISSFT 125
           NNIGRNLIRYQVTDPSDDPINPRPGTLRYGA++I+ KVWITF++ M I LHKPL+ISS+T
Sbjct: 63  NNIGRNLIRYQVTDPSDDPINPRPGTLRYGATMIKSKVWITFQKGMHILLHKPLLISSYT 122

Query: 126 AIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDA 185
           AIDGRGA+VHI+GNACL+V +A+NIIIHGLIIHHC+AQ A QVMGP+SK++SLGHVDGDA
Sbjct: 123 AIDGRGASVHISGNACLVVSKATNIIIHGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDA 182

Query: 186 IRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDWR 245
           IRLV+ASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGH+DGYVRD  
Sbjct: 183 IRLVSASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHEDGYVRDKN 242

Query: 246 MKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK 305
           M+VTV+YNHFGPNCNQRMPRIR+GYAHVANN+YQGWTQYAIGGSMNPSVKSEANLFIASK
Sbjct: 243 MRVTVIYNHFGPNCNQRMPRIRHGYAHVANNLYQGWTQYAIGGSMNPSVKSEANLFIASK 302

Query: 306 SKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVAD 365
            K+     +VWSTG VE  KW FHSV DVFENGASF QIGVGRG V PNY+AQQRF VAD
Sbjct: 303 LKQ-----IVWSTGKVEAAKWKFHSVGDVFENGASFEQIGVGRGGVKPNYSAQQRFPVAD 362

Query: 366 AKWVRSLTTSSGALRCARNSRC 387
           AKWVRSLT+SSGALRC+  SRC
Sbjct: 363 AKWVRSLTSSSGALRCSSKSRC 379

BLAST of MS018818 vs. TAIR 10
Match: AT5G09280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 8.1e-115
Identity = 184/243 (75.72%), Postives = 220/243 (90.53%), Query Frame = 0

Query: 63  MTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISS 122
           MT NIG+ + +Y+VTDPSDDP+NP+PGTLRYGA++++GK WITF+R+M+I LHKPL+ISS
Sbjct: 1   MTGNIGKGVTQYKVTDPSDDPLNPKPGTLRYGATLVKGKKWITFKRNMKIKLHKPLLISS 60

Query: 123 FTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGHVDG 182
           FTA+DGRGA+VHI+G ACL+V RA+++IIHGL IH C+A   + VMGPDSKII LGH+DG
Sbjct: 61  FTALDGRGASVHISGPACLIVYRATDVIIHGLKIHDCKAHPPSSVMGPDSKIIQLGHMDG 120

Query: 183 DAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRD 242
           DAIRLVTA KVWIDHNTLY CEDGLLDVT GSTD+T+SNNWFR+QDKV+LLGHDDGYV+D
Sbjct: 121 DAIRLVTAKKVWIDHNTLYDCEDGLLDVTLGSTDVTVSNNWFRNQDKVMLLGHDDGYVKD 180

Query: 243 WRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIA 302
             M+VTVV+NHFGPNCNQRMPR+R+GYAHVANN YQGWTQYAIGGSM+P VKSE+N F+A
Sbjct: 181 KDMRVTVVFNHFGPNCNQRMPRVRHGYAHVANNYYQGWTQYAIGGSMSPRVKSESNYFVA 240

Query: 303 SKS 306
            KS
Sbjct: 241 PKS 243

BLAST of MS018818 vs. TAIR 10
Match: AT1G11920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 322.8 bits (826), Expect = 3.8e-88
Identity = 180/396 (45.45%), Postives = 236/396 (59.60%), Query Frame = 0

Query: 11  FLSLIS--FALLSAAACSARGLN--------MNAIDRCWRSNPYWRRNRQQLGVCSVGFV 70
           FL++IS  FA  S++   A   N         N ID CWR NPYW  NR+ L  C+VGF 
Sbjct: 5   FLTIISLLFAAFSSSVVEAAYSNGYTIPKLLPNPIDSCWRRNPYWASNRRALADCAVGF- 64

Query: 71  GKMTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGK-VWITFRRDMRITLHKPLM 130
           GK     G+    Y VT+PSDDP NPRPGTLRY  +VI+ K +WITF RDM I L   L+
Sbjct: 65  GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLRY--AVIQSKPLWITFARDMVIVLRNELI 124

Query: 131 ISSFTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISLGH 190
           ++S+  IDGRGA V IA   C+ +   S++IIHG+ IH C+   + +V    + + S   
Sbjct: 125 MNSYKTIDGRGAKVEIAYGPCITIQHVSHVIIHGISIHDCKPGKSGRVRSSPTHVGSRKG 184

Query: 191 VDGDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGY 250
            DGDAI +  +S +WIDH    RC+DGL+DV   ST +TISNN+F   DKV+LLGH+D  
Sbjct: 185 SDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVLHASTAVTISNNYFTQHDKVMLLGHNDNN 244

Query: 251 VRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANL 310
           V D  M+VT+ +NHFGP   +RMPR+R GYAHVANN Y+ W  YAIGGS +P++ SE N 
Sbjct: 245 VEDKIMRVTIAFNHFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSADPTIFSEGNY 304

Query: 311 FIAS---------KSKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVV 370
           F+AS         K  ++ YDW           +W + + +DVF+NGA F  +  G G V
Sbjct: 305 FVASDDPSKKQVTKRIDSGYDW----------KRWKWRTSKDVFKNGAYF--VPSGYGTV 364

Query: 371 TPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
           TP Y   +RF V+    V  LT+S+G L C     C
Sbjct: 365 TPLYGRAERFPVSHGSLVPLLTSSAGPLHCYSGRIC 384

BLAST of MS018818 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 3.0e-85
Identity = 175/383 (45.69%), Postives = 225/383 (58.75%), Query Frame = 0

Query: 5   MASQLFFLSLISFALLSAAACSARGLN-------MNAIDRCWRSNPYWRRNRQQLGVCSV 64
           MAS +  +SL+  A  S    +A   N       +N ID CWR NP W  NRQ L  C+V
Sbjct: 1   MASLVVIVSLLLAAFASPLLETAHSYNVTAPRVSLNPIDACWRRNPKWATNRQALAHCAV 60

Query: 65  GFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKP 124
           G+ GK     G+N   Y VT+PSD+P  P PGTLRY  S  +  +WITF RDM I L   
Sbjct: 61  GY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTLRYAVSQPK-PLWITFARDMVIVLKSQ 120

Query: 125 LMISSFTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPDSKIISL 184
           LMI+S+  IDGRGA V IA   CL + +  ++IIHG+ IH C+A                
Sbjct: 121 LMINSYKTIDGRGAKVEIANGPCLRIRQVKHVIIHGISIHDCKADP-------------- 180

Query: 185 GHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDD 244
             +DGD IR+  ++ VWIDH  L RC DGL+DV   ST +TISNN+F   DKV+LLGHDD
Sbjct: 181 NGMDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVIVSSTAVTISNNYFTQHDKVMLLGHDD 240

Query: 245 GYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEA 304
            Y+ D  M+VT+ +N FGP   +RMPR+R GYAHVANN Y+ W  YAIGGS NP + SE 
Sbjct: 241 SYMGDKDMRVTIAFNTFGPGLIERMPRVRRGYAHVANNRYEKWQMYAIGGSANPIIFSEG 300

Query: 305 NLFIASKSKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQ 364
           N F+A + + ++        G  +  +W + + RDVF NGA F   GV   +V P Y   
Sbjct: 301 NYFVAPEKRSSKQVTKRMMAG-PDSKRWKWGTSRDVFMNGAFFGPPGV---IVRPLYKGG 360

Query: 365 QRFQVADAKWVRSLTTSSGALRC 381
           + F+VA    V SLT+S+G LRC
Sbjct: 361 EGFRVAHGSLVPSLTSSAGPLRC 362

BLAST of MS018818 vs. TAIR 10
Match: AT4G22080.1 (root hair specific 14 )

HSP 1 Score: 312.0 bits (798), Expect = 6.7e-85
Identity = 168/359 (46.80%), Postives = 215/359 (59.89%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MN +D CWR    W  NR+ L  C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 92  RYGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNII 151
           RYG  VI+ K +WITF +DM ITL   LM++S+  IDGRGA V IA   C+ +   +N+I
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 152 IHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDV 211
           +HG+ IH C+      V    + +      DGDAI +  +S +WIDH  L  C DGL+DV
Sbjct: 166 VHGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDV 225

Query: 212 TRGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYA 271
              ST ITISNN+F   DKV+LLGH+D +V+D +MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 272 HVANNMYQGWTQYAIGGSMNPSVKSEANLFIA---SKSKEARYDWVVWSTGNVEETKWNF 331
           HVANN Y  W  YAIGGS +P++ SE N FIA   S SKE     V     N     W +
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNN-----WRW 345

Query: 332 HSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
            + +DVF+NGA F  +  G G ++  Y++ QRF VA    V SLT  +G L C RN  C
Sbjct: 346 RTSKDVFKNGAYF--VPSGYGSISLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393

BLAST of MS018818 vs. TAIR 10
Match: AT4G22090.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.5e-84
Identity = 168/359 (46.80%), Postives = 215/359 (59.89%), Query Frame = 0

Query: 32  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 91
           MN +D CWR    W  NR+ L  C+VGF          N+  Y VT+P D+  NP PG+L
Sbjct: 46  MNPVDSCWRLKSDWDVNREDLADCAVGFGSSTLGGKKGNI--YVVTNPYDNAQNPHPGSL 105

Query: 92  RYGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNII 151
           RYG  VI+ K +WITF +DM ITL   LM++S+  IDGRGA V IA   C+ +   +N+I
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLANELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 152 IHGLIIHHCRAQAAAQVMGPDSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDV 211
           +HG+ IH C+   + +V    + +      DGDAI +  +S VWIDH  L  C DGL+DV
Sbjct: 166 VHGISIHDCKPGKSGKVRSSPTHVGHRKGSDGDAITIFGSSNVWIDHCYLASCTDGLIDV 225

Query: 212 TRGSTDITISNNWFRDQDKVILLGHDDGYVRDWRMKVTVVYNHFGPNCNQRMPRIRYGYA 271
              ST ITISNN+F   DKV+LLGH+D +V+D +MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTAITISNNYFTQHDKVMLLGHNDNFVKDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 272 HVANNMYQGWTQYAIGGSMNPSVKSEANLFIASK---SKEARYDWVVWSTGNVEETKWNF 331
           HVANN Y  W  YAIGGS +P++ SE N FIAS    SKE     V     N     W +
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSYSKEVTKREVKGGWNN-----WRW 345

Query: 332 HSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWVRSLTTSSGALRCARNSRC 387
            +  DVF+NGA F  +  G G +   Y++ QRF VA    V SLT  +G L C RN  C
Sbjct: 346 RTSNDVFKNGAFF--VPSGYGSIPLPYSSAQRFTVAPGNLVPSLTADAGPLNCNRNGPC 393