MS018810 (gene) Bitter gourd (TR) v1

Overview


Name	MS018810
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	zingipain-2-like
Location	scaffold14: 74400 .. 74579 (-)
RNA-Seq Expression	MS018810
Synteny	MS018810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CATGCAGTTCTGATTGTAGGCTTTGGAAAGGACAATGATGGTAATAGGTATTGGATTATAAGAAACTCATGGGGAGGAGGCTGGTCAGATGATGGCTATGGAAAAATCAGCCAAGACAGTGTTAATACGAGTGAGGGACCAAAATACTTGTTGGAAAGGTTATCCTATCCAACAATGAAA

mRNA sequence

CATGCAGTTCTGATTGTAGGCTTTGGAAAGGACAATGATGGTAATAGGTATTGGATTATAAGAAACTCATGGGGAGGAGGCTGGTCAGATGATGGCTATGGAAAAATCAGCCAAGACAGTGTTAATACGAGTGAGGGACCAAAATACTTGTTGGAAAGGTTATCCTATCCAACAATGAAA

Coding sequence (CDS)

CATGCAGTTCTGATTGTAGGCTTTGGAAAGGACAATGATGGTAATAGGTATTGGATTATAAGAAACTCATGGGGAGGAGGCTGGTCAGATGATGGCTATGGAAAAATCAGCCAAGACAGTGTTAATACGAGTGAGGGACCAAAATACTTGTTGGAAAGGTTATCCTATCCAACAATGAAA

Protein sequence

HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTMK

Homology

[Close]

BLAST of MS018810 vs. NCBI nr
Match: XP_022949074.1 (zingipain-2-like [Cucurbita moschata])

HSP 1 Score: 86.3 bits (212), Expect = 1.0e-13
Identity = 38/60 (63.33%), Postives = 49/60 (81.67%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTMK 60
           HAVL+VGFG+ N+  +YWII+NSWG  W D GYGKISQ+ VNT +GP+ L+ERLSYP ++
Sbjct: 150 HAVLVVGFGEHNE-QKYWIIKNSWGERWKDHGYGKISQNIVNTIQGPRRLIERLSYPIIE 208

[Close]

BLAST of MS018810 vs. NCBI nr
Match: XP_023524541.1 (zingipain-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 67.8 bits (164), Expect = 3.7e-08
Identity = 30/46 (65.22%), Postives = 37/46 (80.43%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEG 47
           HAVL+VGFG+ N+  +YWII+NSWG  W D GYGKISQ+ VNT +G
Sbjct: 128 HAVLVVGFGEHNE-QKYWIIKNSWGERWKDHGYGKISQNIVNTIQG 172

[Close]

BLAST of MS018810 vs. NCBI nr
Match: PKU74158.1 (KDEL-tailed cysteine endopeptidase CEP1 [Dendrobium catenatum])

HSP 1 Score: 67.0 bits (162), Expect = 6.3e-08
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           HAV  +G+G D DG +YW+++NSWG  W DDGY KI +D ++  EG   L  + SYPT+
Sbjct: 143 HAVTAIGYGIDTDGTKYWLLKNSWGTSWGDDGYIKIQRD-IDAVEGLCGLATQASYPTI 200

[Close]

BLAST of MS018810 vs. NCBI nr
Match: XP_028552917.1 (senescence-specific cysteine protease SAG39-like [Dendrobium catenatum])

HSP 1 Score: 67.0 bits (162), Expect = 6.3e-08
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           HAV  +G+G D DG +YW+++NSWG  W DDGY KI +D ++  EG   L  + SYPT+
Sbjct: 267 HAVTAIGYGIDTDGTKYWLLKNSWGTSWGDDGYIKIQRD-IDAVEGLCGLATQASYPTI 324

[Close]

BLAST of MS018810 vs. NCBI nr
Match: KAB1202498.1 (hypothetical protein CJ030_MR8G018303 [Morella rubra] >KAB1222056.1 hypothetical protein CJ030_MR2G017955 [Morella rubra])

HSP 1 Score: 66.6 bits (161), Expect = 8.2e-08
Identity = 27/59 (45.76%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           H V +VG+G D DG +YW+++NSWG GW +DGY K+ +D ++  EG   +    SYPT+
Sbjct: 292 HGVTVVGYGADADGTKYWLVKNSWGTGWGEDGYIKMQRD-IDAKEGLCGIAMEASYPTV 349

[Close]

BLAST of MS018810 vs. ExPASy Swiss-Prot
Match: P80884 (Ananain OS=Ananas comosus OX=4615 GN=AN1 PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 2.0e-09
Identity = 25/60 (41.67%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTMK 60
           HA++I+G+G+D+ G ++WI+RNSWG GW + GY ++++D V++S G   +     YPT++
Sbjct: 279 HAIVIIGYGQDSSGKKFWIVRNSWGAGWGEGGYIRLARD-VSSSFGLCGIAMDPLYPTLQ 337

[Close]

BLAST of MS018810 vs. ExPASy Swiss-Prot
Match: Q9FJ47 (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=SAG12 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-08
Identity = 26/59 (44.07%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           HAV  +G+G+  +G++YWII+NSWG  W + GY +I +D V   +G   L  + SYPT+
Sbjct: 289 HAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKD-VKDKQGLCGLAMKASYPTI 346

[Close]

BLAST of MS018810 vs. ExPASy Swiss-Prot
Match: O23791 (Fruit bromelain OS=Ananas comosus OX=4615 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.2e-08
Identity = 23/60 (38.33%), Postives = 41/60 (68.33%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTMK 60
           HA+ I+G+G+D+ G +YWI+RNSWG  W + GY ++++  V++S G   +     +PT++
Sbjct: 279 HAITIIGYGQDSSGTKYWIVRNSWGSSWGEGGYVRMAR-GVSSSSGVCGIAMAPLFPTLQ 337

[Close]

BLAST of MS018810 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.9e-08
Identity = 25/58 (43.10%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPT 59
           H V+IVG+G+  +G +YWI+RNSWG  W + GY +I +  ++ +EG   +    SYPT
Sbjct: 287 HGVVIVGYGETKNGTKYWIVRNSWGPEWGEGGYVRIER-GISENEGRCGIAMEASYPT 343

[Close]

BLAST of MS018810 vs. ExPASy Swiss-Prot
Match: A2XQE8 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_14861 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.2e-07
Identity = 21/58 (36.21%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPT 59
           H ++ +G+GK +DG +YW+++NSWG  W ++G+ ++ +D ++   G   L    SYPT
Sbjct: 282 HGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKD-ISDKRGMCGLAMEPSYPT 338

[Close]

BLAST of MS018810 vs. ExPASy TrEMBL
Match: A0A6J1GBS9 (zingipain-2-like OS=Cucurbita moschata OX=3662 GN=LOC111452536 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.8e-14
Identity = 38/60 (63.33%), Postives = 49/60 (81.67%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTMK 60
           HAVL+VGFG+ N+  +YWII+NSWG  W D GYGKISQ+ VNT +GP+ L+ERLSYP ++
Sbjct: 150 HAVLVVGFGEHNE-QKYWIIKNSWGERWKDHGYGKISQNIVNTIQGPRRLIERLSYPIIE 208

[Close]

BLAST of MS018810 vs. ExPASy TrEMBL
Match: A0A7S3CIY7 (Hypothetical protein OS=Strombidium rassoulzadegani OX=1082188 GN=SRAS04492_LOCUS1471 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.3e-08
Identity = 27/57 (47.37%), Postives = 39/57 (68.42%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYP 58
           HAVL VG+G D+DG+ YW+++NSWGG W + GY ++ +D     EG   +L+  SYP
Sbjct: 279 HAVLAVGYGTDSDGSDYWLVKNSWGGDWGEHGYFRVLRDMSKEDEGTCGILQTPSYP 335

[Close]

BLAST of MS018810 vs. ExPASy TrEMBL
Match: A0A2I0WER7 (KDEL-tailed cysteine endopeptidase CEP1 OS=Dendrobium catenatum OX=906689 GN=CEP1 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-08
Identity = 29/59 (49.15%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           HAV  +G+G D DG +YW+++NSWG  W DDGY KI +D ++  EG   L  + SYPT+
Sbjct: 143 HAVTAIGYGIDTDGTKYWLLKNSWGTSWGDDGYIKIQRD-IDAVEGLCGLATQASYPTI 200

[Close]

BLAST of MS018810 vs. ExPASy TrEMBL
Match: A0A6A1WCP8 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR2G017955 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.0e-08
Identity = 27/59 (45.76%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           H V +VG+G D DG +YW+++NSWG GW +DGY K+ +D ++  EG   +    SYPT+
Sbjct: 292 HGVTVVGYGADADGTKYWLVKNSWGTGWGEDGYIKMQRD-IDAKEGLCGIAMEASYPTV 349

[Close]

BLAST of MS018810 vs. ExPASy TrEMBL
Match: A0A199VZY0 (Senescence-specific cysteine protease SAG39 OS=Ananas comosus OX=4615 GN=LOC109722379 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.2e-08
Identity = 29/58 (50.00%), Postives = 41/58 (70.69%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPT 59
           HAV  +G+G  +DG +YW+I+NSWG  W +DGY +I +D V++SEG   L  + SYPT
Sbjct: 286 HAVTAIGYGTADDGTKYWLIKNSWGTAWGEDGYMRIQRD-VDSSEGMCGLAMQASYPT 342

[Close]

BLAST of MS018810 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 60.1 bits (144), Expect = 7.1e-10
Identity = 26/59 (44.07%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPTM 60
           HAV  +G+G+  +G++YWII+NSWG  W + GY +I +D V   +G   L  + SYPT+
Sbjct: 289 HAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKD-VKDKQGLCGLAMKASYPTI 346

[Close]

BLAST of MS018810 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-09
Identity = 25/58 (43.10%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYPT 59
           H V+IVG+G+  +G +YWI+RNSWG  W + GY +I +  ++ +EG   +    SYPT
Sbjct: 287 HGVVIVGYGETKNGTKYWIVRNSWGPEWGEGGYVRIER-GISENEGRCGIAMEASYPT 343

[Close]

BLAST of MS018810 vs. TAIR 10
Match: AT2G27420.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.8e-08
Identity = 24/57 (42.11%), Postives = 36/57 (63.16%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYP 58
           HAV IVG+G   +G +YW+++NSWG  W ++GY +I +D V+  +G   L     YP
Sbjct: 291 HAVTIVGYGMSEEGTKYWVVKNSWGETWGENGYMRIKRD-VDAPQGMCGLAILAFYP 346

[Close]

BLAST of MS018810 vs. TAIR 10
Match: AT3G49340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.3e-08
Identity = 24/57 (42.11%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYP 58
           HAV IVG+G   +G +YW+++NSWG  W ++GY +I +D V++ +G   L     YP
Sbjct: 284 HAVTIVGYGVSEEGIKYWLLKNSWGESWGENGYMRIMRD-VDSPQGMCGLASLAYYP 339

[Close]

BLAST of MS018810 vs. TAIR 10
Match: AT1G29080.1 (Papain family cysteine protease )

HSP 1 Score: 52.4 bits (124), Expect = 1.5e-07
Identity = 23/57 (40.35%), Postives = 36/57 (63.16%), Query Frame = 0


Query: 1   HAVLIVGFGKDNDGNRYWIIRNSWGGGWSDDGYGKISQDSVNTSEGPKYLLERLSYP 58
           HAV +VG+G   +G +YW+ +NSWG  W ++GY +I +D V   +G   + +  SYP
Sbjct: 289 HAVTLVGYGTSPEGMKYWLAKNSWGKTWGENGYIRIRRD-VEWPQGMCGVAQYASYP 344

The following BLAST results are available for this feature:

BLAST of MS018810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949074.1	1.0e-13	63.33	zingipain-2-like [Cucurbita moschata]	[more]
XP_023524541.1	3.7e-08	65.22	zingipain-2-like [Cucurbita pepo subsp. pepo]	[more]
PKU74158.1	6.3e-08	49.15	KDEL-tailed cysteine endopeptidase CEP1 [Dendrobium catenatum]	[more]
XP_028552917.1	6.3e-08	49.15	senescence-specific cysteine protease SAG39-like [Dendrobium catenatum]	[more]
KAB1202498.1	8.2e-08	45.76	hypothetical protein CJ030_MR8G018303 [Morella rubra] >KAB1222056.1 hypothetical...	[more]

BLAST of MS018810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P80884	2.0e-09	41.67	Ananain OS=Ananas comosus OX=4615 GN=AN1 PE=1 SV=2	[more]
Q9FJ47	1.0e-08	44.07	Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
O23791	2.2e-08	38.33	Fruit bromelain OS=Ananas comosus OX=4615 PE=1 SV=1	[more]
Q9STL5	2.9e-08	43.10	KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ...	[more]
A2XQE8	3.2e-07	36.21	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of MS018810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBS9	4.8e-14	63.33	zingipain-2-like OS=Cucurbita moschata OX=3662 GN=LOC111452536 PE=3 SV=1	[more]
A0A7S3CIY7	2.3e-08	47.37	Hypothetical protein OS=Strombidium rassoulzadegani OX=1082188 GN=SRAS04492_LOCU...	[more]
A0A2I0WER7	3.0e-08	49.15	KDEL-tailed cysteine endopeptidase CEP1 OS=Dendrobium catenatum OX=906689 GN=CEP...	[more]
A0A6A1WCP8	4.0e-08	45.76	Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR2G017955 PE=3 SV=1	[more]
A0A199VZY0	5.2e-08	50.00	Senescence-specific cysteine protease SAG39 OS=Ananas comosus OX=4615 GN=LOC1097...	[more]

BLAST of MS018810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45890.1	7.1e-10	44.07	senescence-associated gene 12	[more]
AT3G48350.1	2.1e-09	43.10	Cysteine proteinases superfamily protein	[more]
AT2G27420.1	1.8e-08	42.11	Cysteine proteinases superfamily protein	[more]
AT3G49340.1	2.3e-08	42.11	Cysteine proteinases superfamily protein	[more]
AT1G29080.1	1.5e-07	40.35	Papain family cysteine protease	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 1..42 e-value: 1.2E-12 score: 48.4
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 1..60 e-value: 1.9E-16 score: 62.6
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..46

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018810.1	MS018810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity