MS018754 (gene) Bitter gourd (TR) v1

Overview


Name	MS018754
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Germin-like protein
Location	scaffold313: 1720298 .. 1720846 (-)
RNA-Seq Expression	MS018754
Synteny	MS018754

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAATAAAAGTGAATGGATTTGCGTGCAAGGACGAGGCGAACGTAAGCGCATCAGACTTCTTCTTCGCGGGACTAGCCAACGCCCCCGCCATCAACAACTCGCTCGGCTTCGCGGCGACGGTGGCCGACGTCAACGCAATCCCCGGCCTCAACACCCTCGGCCTTTCCCTCGCCCGCGTCGAGTACTCACCGGGCGGCGGCCTCGTCCCCCCTCACGTGCATCCACGCGCCACCGAGGTTGTGTTCGTGCTCGACGGCGTGCTGGACGTGGGCTTCATCACCACCGCCAACAAACTGGTCGCAAAAACCATAAAAAAAGGCGACGTGTTCGTGTTCCCCAAGGGCCTGCTCCACTTCCAGCGCAACAGTTACGACAGGCCTGCCTCCGCTTTCATCGTCTTCAACAGCCAGCTGTTCGGAACCCAGAGTGTGGCCCCCGCCTTGTTCGGTGCCTCGCCCGCCGTTGACGACGCCGTCCTCGCCGGAACTTTTCAGATTGGTGCTGACGTGGTGGCCCAGATTAAATCCAACATAGCTCCTACCAAAAAG

mRNA sequence

GGAATAAAAGTGAATGGATTTGCGTGCAAGGACGAGGCGAACGTAAGCGCATCAGACTTCTTCTTCGCGGGACTAGCCAACGCCCCCGCCATCAACAACTCGCTCGGCTTCGCGGCGACGGTGGCCGACGTCAACGCAATCCCCGGCCTCAACACCCTCGGCCTTTCCCTCGCCCGCGTCGAGTACTCACCGGGCGGCGGCCTCGTCCCCCCTCACGTGCATCCACGCGCCACCGAGGTTGTGTTCGTGCTCGACGGCGTGCTGGACGTGGGCTTCATCACCACCGCCAACAAACTGGTCGCAAAAACCATAAAAAAAGGCGACGTGTTCGTGTTCCCCAAGGGCCTGCTCCACTTCCAGCGCAACAGTTACGACAGGCCTGCCTCCGCTTTCATCGTCTTCAACAGCCAGCTGTTCGGAACCCAGAGTGTGGCCCCCGCCTTGTTCGGTGCCTCGCCCGCCGTTGACGACGCCGTCCTCGCCGGAACTTTTCAGATTGGTGCTGACGTGGTGGCCCAGATTAAATCCAACATAGCTCCTACCAAAAAG

Coding sequence (CDS)

GGAATAAAAGTGAATGGATTTGCGTGCAAGGACGAGGCGAACGTAAGCGCATCAGACTTCTTCTTCGCGGGACTAGCCAACGCCCCCGCCATCAACAACTCGCTCGGCTTCGCGGCGACGGTGGCCGACGTCAACGCAATCCCCGGCCTCAACACCCTCGGCCTTTCCCTCGCCCGCGTCGAGTACTCACCGGGCGGCGGCCTCGTCCCCCCTCACGTGCATCCACGCGCCACCGAGGTTGTGTTCGTGCTCGACGGCGTGCTGGACGTGGGCTTCATCACCACCGCCAACAAACTGGTCGCAAAAACCATAAAAAAAGGCGACGTGTTCGTGTTCCCCAAGGGCCTGCTCCACTTCCAGCGCAACAGTTACGACAGGCCTGCCTCCGCTTTCATCGTCTTCAACAGCCAGCTGTTCGGAACCCAGAGTGTGGCCCCCGCCTTGTTCGGTGCCTCGCCCGCCGTTGACGACGCCGTCCTCGCCGGAACTTTTCAGATTGGTGCTGACGTGGTGGCCCAGATTAAATCCAACATAGCTCCTACCAAAAAG

Protein sequence

GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARVEYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQRNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAPTKK

Homology

[Close]

BLAST of MS018754 vs. NCBI nr
Match: XP_022146354.1 (germin-like protein subfamily 2 member 4 [Momordica charantia])

HSP 1 Score: 293.1 bits (749), Expect = 1.6e-75
Identity = 151/183 (82.51%), Postives = 160/183 (87.43%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CKDEANV+A DFFFAGLANA AINNS+G A T A+V+ IPGLNTLG+SLARV
Sbjct: 46  GIKVNGFPCKDEANVTALDFFFAGLANAAAINNSVGSAVTAANVDKIPGLNTLGVSLARV 105

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPHVHPRATE+VFVLDG LDVGFITTANKLVAKTIKKG+VFVFPKGLLHFQ
Sbjct: 106 DYSPAGGLNPPHVHPRATEIVFVLDGELDVGFITTANKLVAKTIKKGEVFVFPKGLLHFQ 165

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           RNS D+PAS    FNSQL GTQSVA ALFGASPAVDDAVLAG FQIG  VV QIKS IAP
Sbjct: 166 RNSKDKPASVIAAFNSQLPGTQSVAAALFGASPAVDDAVLAGAFQIGGVVVGQIKSKIAP 225

Query: 181 TKK 184
            KK
Sbjct: 226 KKK 228

[Close]

BLAST of MS018754 vs. NCBI nr
Match: XP_038877903.1 (germin-like protein subfamily 2 member 4 [Benincasa hispida] >XP_038877904.1 germin-like protein subfamily 2 member 4 [Benincasa hispida])

HSP 1 Score: 270.4 bits (690), Expect = 1.1e-68
Identity = 133/183 (72.68%), Postives = 154/183 (84.15%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CK++ N++ASDFFFAGLA   AINNS+G A T+A+V+ IPGLNTLG+S AR+
Sbjct: 41  GIKVNGFPCKEDTNITASDFFFAGLAKPAAINNSVGSAVTLANVDKIPGLNTLGISFARI 100

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPH+HPRATE+VFVLDG LDVGFITTANKL++KTIKKG++FVFPKGLLHFQ
Sbjct: 101 DYSPDGGLNPPHIHPRATEIVFVLDGELDVGFITTANKLISKTIKKGEIFVFPKGLLHFQ 160

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N  D+PAS    FNSQL GT SVA ALF +SP VDD VLA TFQIGAD V +IKS IAP
Sbjct: 161 QNKKDKPASVIAAFNSQLPGTLSVAAALFSSSPPVDDGVLARTFQIGADEVGEIKSKIAP 220

Query: 181 TKK 184
            KK
Sbjct: 221 KKK 223

[Close]

BLAST of MS018754 vs. NCBI nr
Match: KAA0050257.1 (germin-like protein subfamily 2 member 4 [Cucumis melo var. makuwa] >TYJ98151.1 germin-like protein subfamily 2 member 4 [Cucumis melo var. makuwa])

HSP 1 Score: 266.5 bits (680), Expect = 1.6e-67
Identity = 132/183 (72.13%), Postives = 152/183 (83.06%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G KVNGF CK + NV+ASDFFFAGLAN  AINNS+G   T A+V+ IPGLNTLG+SLAR+
Sbjct: 4   GTKVNGFPCKADTNVTASDFFFAGLANPAAINNSVGSVVTPANVDKIPGLNTLGVSLARI 63

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPH+HPRATE+VF+LDG LDVGF+TTANKL++KTIKKG+VFVFPKGLLHFQ
Sbjct: 64  DYSPDGGLNPPHIHPRATEIVFILDGELDVGFVTTANKLISKTIKKGEVFVFPKGLLHFQ 123

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N  D+PAS    FNSQL GT SVA ALF +SPAVDD +LA TFQIG D V +IKS IAP
Sbjct: 124 QNKKDKPASVLSAFNSQLPGTVSVAAALFSSSPAVDDGLLAKTFQIGTDEVGEIKSKIAP 183

Query: 181 TKK 184
            KK
Sbjct: 184 KKK 186

[Close]

BLAST of MS018754 vs. NCBI nr
Match: XP_008466192.1 (PREDICTED: germin-like protein subfamily 2 member 4 [Cucumis melo])

HSP 1 Score: 266.2 bits (679), Expect = 2.1e-67
Identity = 133/183 (72.68%), Postives = 151/183 (82.51%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G KVNGF CK   NV+ SDFFFAGLAN  AINNS+G A T A+V+ IPGLNTLG+SLAR+
Sbjct: 41  GTKVNGFPCKAATNVTTSDFFFAGLANPAAINNSVGSAVTPANVDKIPGLNTLGVSLARI 100

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPH+HPRATE+VF+LDG LDVGFITTANKL++KTIKKG+VFVFPKGLLHFQ
Sbjct: 101 DYSPNGGLNPPHIHPRATEIVFILDGELDVGFITTANKLISKTIKKGEVFVFPKGLLHFQ 160

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N  D+PAS    FNSQL GT SVA ALF +SPAVDD +LA TFQIG D V +IKS IAP
Sbjct: 161 QNKKDKPASVLSAFNSQLPGTVSVAAALFSSSPAVDDGLLAKTFQIGTDEVGEIKSKIAP 220

Query: 181 TKK 184
            KK
Sbjct: 221 KKK 223

[Close]

BLAST of MS018754 vs. NCBI nr
Match: XP_008466206.1 (PREDICTED: germin-like protein subfamily 2 member 4 [Cucumis melo])

HSP 1 Score: 262.7 bits (670), Expect = 2.4e-66
Identity = 125/181 (69.06%), Postives = 153/181 (84.53%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CK++ N++ASDFFFAGLA    +NNS+GFAAT A+V+++PGLNTLG+SL RV
Sbjct: 41  GIKVNGFPCKEDTNITASDFFFAGLATPAVVNNSIGFAATQANVDSVPGLNTLGISLNRV 100

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           EYSP GGLVPPH HPRATE++F+L+G LDVGFITTANKL++KTIKKG+VFVFP GLLH+Q
Sbjct: 101 EYSPNGGLVPPHTHPRATEIIFILEGELDVGFITTANKLISKTIKKGEVFVFPVGLLHYQ 160

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+ D+PASA + FNSQL G   VAPALF +SPA+D+ VL  TFQIG D V +IKS  AP
Sbjct: 161 QNNKDKPASAIVAFNSQLPGILVVAPALFSSSPAIDNDVLGRTFQIGTDKVGEIKSRFAP 220

Query: 181 T 182
           +
Sbjct: 221 S 221

[Close]

BLAST of MS018754 vs. ExPASy Swiss-Prot
Match: Q9M263 (Germin-like protein subfamily 2 member 4 OS=Arabidopsis thaliana OX=3702 GN=GLP10 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.3e-59
Identity = 116/183 (63.39%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CK E+N++ASDFFFAG+     +NN++G A T A+V  I GLNTLG+SLAR+
Sbjct: 38  GIKVNGFTCKPESNITASDFFFAGIGKPAVVNNTVGSAVTGANVEKIAGLNTLGVSLARI 97

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEV+FVL+G LDVGFITTANKL AKT+KKG+VFVFP+GL+H+Q
Sbjct: 98  DYAP-GGLNPPHTHPRATEVIFVLEGELDVGFITTANKLFAKTVKKGEVFVFPRGLIHYQ 157

Query: 121 RNS-YDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIA 180
           +N+   +PAS    FNSQL GTQS+A  LF A+PA+ D VL  TFQIG   + +IKS  A
Sbjct: 158 KNNDKAKPASVISAFNSQLPGTQSIAATLFTATPAIPDHVLTTTFQIGTKEIEKIKSKFA 217

Query: 181 PTK 183
           P K
Sbjct: 218 PKK 219

[Close]

BLAST of MS018754 vs. ExPASy Swiss-Prot
Match: P94014 (Germin-like protein subfamily 2 member 1 OS=Arabidopsis thaliana OX=3702 GN=GLP4 PE=2 SV=2)

HSP 1 Score: 218.0 bits (554), Expect = 8.8e-56
Identity = 109/182 (59.89%), Postives = 134/182 (73.63%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIK+NGF CKD A V+++DFF  GLA     NN+ G   T A+V  IPGLNTLG+SL+R+
Sbjct: 39  GIKINGFPCKDAATVTSADFFSQGLAKPGLTNNTFGALVTGANVMTIPGLNTLGVSLSRI 98

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEVVFVL+G LDVGF+TTANKL+++++KKGDVF FPKGL+HFQ
Sbjct: 99  DYAP-GGLNPPHTHPRATEVVFVLEGTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQ 158

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+ D PAS    FNSQL GTQS+   LFG++P V D +LA  FQ     V  IKS   P
Sbjct: 159 KNNGDVPASVIAAFNSQLPGTQSLGATLFGSTPPVPDNILAQAFQTSPGTVKHIKSKFQP 218

Query: 181 TK 183
            K
Sbjct: 219 KK 219

[Close]

BLAST of MS018754 vs. ExPASy Swiss-Prot
Match: Q6I544 (Germin-like protein 5-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0277500 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 5.7e-55
Identity = 112/182 (61.54%), Postives = 134/182 (73.63%), Query Frame = 0


Query: 2   IKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARVE 61
           +KVNGFACK  A V+  DF+F GLA A   NN+ G   T A+V  +PGLNTLG+S++R++
Sbjct: 43  VKVNGFACK--AAVTEDDFYFKGLAAAGNTNNTYGSVVTGANVEKLPGLNTLGVSMSRID 102

Query: 62  YSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQR 121
           Y+P GGL PPH HPRATE+VFVL G LDVGFITTANKL  KTI  GDVFVFP+GLLHFQ+
Sbjct: 103 YAP-GGLNPPHTHPRATEMVFVLQGTLDVGFITTANKLYTKTISAGDVFVFPRGLLHFQK 162

Query: 122 NSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAPT 181
           N+ D PA+    FNSQL GTQS+A  LF ASP V D VL   FQ+G   V +IKS +AP 
Sbjct: 163 NNGDTPAAVISAFNSQLPGTQSLAMTLFAASPEVPDGVLTKAFQVGTKEVEKIKSRLAPK 221

Query: 182 KK 184
           K+
Sbjct: 223 KR 221

[Close]

BLAST of MS018754 vs. ExPASy Swiss-Prot
Match: Q9FZ27 (Germin-like protein subfamily 2 member 2 OS=Arabidopsis thaliana OX=3702 GN=At1g02335 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 9.7e-55
Identity = 108/182 (59.34%), Postives = 137/182 (75.27%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G K+NGF CK+  N++ SDFFFAG++    IN+++G A T A+V  IPGLNTL +SLAR+
Sbjct: 39  GTKLNGFPCKETLNITESDFFFAGISKPAVINSTMGSAVTGANVEKIPGLNTLSVSLARI 98

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEVV+VL+G L+VGFITTANKL  KTIK G+VFVFP+GL+HFQ
Sbjct: 99  DYAP-GGLNPPHTHPRATEVVYVLEGELEVGFITTANKLFTKTIKIGEVFVFPRGLVHFQ 158

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+   PAS    FNSQL GT SVA  LF A PA+ + VL  TFQ+G+ +V +IK  +A 
Sbjct: 159 KNNGKSPASVLSAFNSQLPGTASVAATLFAAEPALPEDVLTKTFQVGSKMVDKIKERLAT 218

Query: 181 TK 183
            K
Sbjct: 219 KK 219

[Close]

BLAST of MS018754 vs. ExPASy Swiss-Prot
Match: Q94EG3 (Nectarin-1 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NECI PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 4.5e-52
Identity = 107/182 (58.79%), Postives = 133/182 (73.08%), Query Frame = 0


Query: 2   IKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARVE 61
           +KVNGF CK   N +A+DF    ++   A NN  G   T A+V  +PGLNTLG+SLAR++
Sbjct: 50  VKVNGFPCK--TNFTAADFSSLAISKPGATNNKFGSVVTTANVEQVPGLNTLGVSLARID 109

Query: 62  YSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQR 121
           Y+P GG+ PPH HPRA+E+VFV++G LDVGFITTAN LV+K I KG+VFVFP+GL+HFQ+
Sbjct: 110 YAP-GGINPPHTHPRASEMVFVMEGELDVGFITTANVLVSKKIIKGEVFVFPRGLVHFQK 169

Query: 122 NSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAPT 181
           N+ + PA+    FNSQL GTQS+   LFGASP V D VLA TFQI  + V QIKS  AP 
Sbjct: 170 NNGEVPAAVISAFNSQLPGTQSIPITLFGASPPVPDDVLAQTFQINTEDVQQIKSKFAPV 228

Query: 182 KK 184
           KK
Sbjct: 230 KK 228

[Close]

BLAST of MS018754 vs. ExPASy TrEMBL
Match: A0A6J1CXW0 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111015585 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 7.9e-76
Identity = 151/183 (82.51%), Postives = 160/183 (87.43%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CKDEANV+A DFFFAGLANA AINNS+G A T A+V+ IPGLNTLG+SLARV
Sbjct: 46  GIKVNGFPCKDEANVTALDFFFAGLANAAAINNSVGSAVTAANVDKIPGLNTLGVSLARV 105

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPHVHPRATE+VFVLDG LDVGFITTANKLVAKTIKKG+VFVFPKGLLHFQ
Sbjct: 106 DYSPAGGLNPPHVHPRATEIVFVLDGELDVGFITTANKLVAKTIKKGEVFVFPKGLLHFQ 165

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           RNS D+PAS    FNSQL GTQSVA ALFGASPAVDDAVLAG FQIG  VV QIKS IAP
Sbjct: 166 RNSKDKPASVIAAFNSQLPGTQSVAAALFGASPAVDDAVLAGAFQIGGVVVGQIKSKIAP 225

Query: 181 TKK 184
            KK
Sbjct: 226 KKK 228

[Close]

BLAST of MS018754 vs. ExPASy TrEMBL
Match: A0A5A7U7A8 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00310 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.0e-68
Identity = 132/183 (72.13%), Postives = 152/183 (83.06%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G KVNGF CK + NV+ASDFFFAGLAN  AINNS+G   T A+V+ IPGLNTLG+SLAR+
Sbjct: 4   GTKVNGFPCKADTNVTASDFFFAGLANPAAINNSVGSVVTPANVDKIPGLNTLGVSLARI 63

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPH+HPRATE+VF+LDG LDVGF+TTANKL++KTIKKG+VFVFPKGLLHFQ
Sbjct: 64  DYSPDGGLNPPHIHPRATEIVFILDGELDVGFVTTANKLISKTIKKGEVFVFPKGLLHFQ 123

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N  D+PAS    FNSQL GT SVA ALF +SPAVDD +LA TFQIG D V +IKS IAP
Sbjct: 124 QNKKDKPASVLSAFNSQLPGTVSVAAALFSSSPAVDDGLLAKTFQIGTDEVGEIKSKIAP 183

Query: 181 TKK 184
            KK
Sbjct: 184 KKK 186

[Close]

BLAST of MS018754 vs. ExPASy TrEMBL
Match: A0A1S3CQM8 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503679 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 1.0e-67
Identity = 133/183 (72.68%), Postives = 151/183 (82.51%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G KVNGF CK   NV+ SDFFFAGLAN  AINNS+G A T A+V+ IPGLNTLG+SLAR+
Sbjct: 41  GTKVNGFPCKAATNVTTSDFFFAGLANPAAINNSVGSAVTPANVDKIPGLNTLGVSLARI 100

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +YSP GGL PPH+HPRATE+VF+LDG LDVGFITTANKL++KTIKKG+VFVFPKGLLHFQ
Sbjct: 101 DYSPNGGLNPPHIHPRATEIVFILDGELDVGFITTANKLISKTIKKGEVFVFPKGLLHFQ 160

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N  D+PAS    FNSQL GT SVA ALF +SPAVDD +LA TFQIG D V +IKS IAP
Sbjct: 161 QNKKDKPASVLSAFNSQLPGTVSVAAALFSSSPAVDDGLLAKTFQIGTDEVGEIKSKIAP 220

Query: 181 TKK 184
            KK
Sbjct: 221 KKK 223

[Close]

BLAST of MS018754 vs. ExPASy TrEMBL
Match: A0A1S3CQN9 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503687 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.2e-66
Identity = 125/181 (69.06%), Postives = 153/181 (84.53%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CK++ N++ASDFFFAGLA    +NNS+GFAAT A+V+++PGLNTLG+SL RV
Sbjct: 41  GIKVNGFPCKEDTNITASDFFFAGLATPAVVNNSIGFAATQANVDSVPGLNTLGISLNRV 100

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           EYSP GGLVPPH HPRATE++F+L+G LDVGFITTANKL++KTIKKG+VFVFP GLLH+Q
Sbjct: 101 EYSPNGGLVPPHTHPRATEIIFILEGELDVGFITTANKLISKTIKKGEVFVFPVGLLHYQ 160

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+ D+PASA + FNSQL G   VAPALF +SPA+D+ VL  TFQIG D V +IKS  AP
Sbjct: 161 QNNKDKPASAIVAFNSQLPGILVVAPALFSSSPAIDNDVLGRTFQIGTDKVGEIKSRFAP 220

Query: 181 T 182
           +
Sbjct: 221 S 221

[Close]

BLAST of MS018754 vs. ExPASy TrEMBL
Match: A0A6J1JZY0 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489804 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 7.5e-66
Identity = 129/183 (70.49%), Postives = 152/183 (83.06%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G KVNGF CK++AN++A+DFFF GLANA  INNS+G   T A+VN IPGLNTLG+SLAR+
Sbjct: 42  GTKVNGFPCKEDANITAADFFFTGLANAATINNSVGSTVTPANVNKIPGLNTLGVSLARI 101

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+  GGL PPHVHPRATE+VFVLDG LDVGFITTANKL++KTIKKG++FVFPKGLLHFQ
Sbjct: 102 DYAADGGLNPPHVHPRATEIVFVLDGELDVGFITTANKLISKTIKKGEIFVFPKGLLHFQ 161

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+ D+PAS    FNSQL GT SVA ALF +SPAVD+ VLA  FQI A+VV +IKS IA 
Sbjct: 162 QNNKDKPASVIAAFNSQLPGTVSVAAALFSSSPAVDNGVLARAFQIDAEVVGEIKSKIAA 221

Query: 181 TKK 184
            KK
Sbjct: 222 KKK 224

[Close]

BLAST of MS018754 vs. TAIR 10
Match: AT3G62020.2 (germin-like protein 10 )

HSP 1 Score: 230.7 bits (587), Expect = 9.3e-61
Identity = 116/183 (63.39%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CK E+N++ASDFFFAG+     +NN++G A T A+V  I GLNTLG+SLAR+
Sbjct: 9   GIKVNGFTCKPESNITASDFFFAGIGKPAVVNNTVGSAVTGANVEKIAGLNTLGVSLARI 68

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEV+FVL+G LDVGFITTANKL AKT+KKG+VFVFP+GL+H+Q
Sbjct: 69  DYAP-GGLNPPHTHPRATEVIFVLEGELDVGFITTANKLFAKTVKKGEVFVFPRGLIHYQ 128

Query: 121 RNS-YDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIA 180
           +N+   +PAS    FNSQL GTQS+A  LF A+PA+ D VL  TFQIG   + +IKS  A
Sbjct: 129 KNNDKAKPASVISAFNSQLPGTQSIAATLFTATPAIPDHVLTTTFQIGTKEIEKIKSKFA 188

Query: 181 PTK 183
           P K
Sbjct: 189 PKK 190

[Close]

BLAST of MS018754 vs. TAIR 10
Match: AT3G62020.1 (germin-like protein 10 )

HSP 1 Score: 230.7 bits (587), Expect = 9.3e-61
Identity = 116/183 (63.39%), Postives = 143/183 (78.14%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIKVNGF CK E+N++ASDFFFAG+     +NN++G A T A+V  I GLNTLG+SLAR+
Sbjct: 38  GIKVNGFTCKPESNITASDFFFAGIGKPAVVNNTVGSAVTGANVEKIAGLNTLGVSLARI 97

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEV+FVL+G LDVGFITTANKL AKT+KKG+VFVFP+GL+H+Q
Sbjct: 98  DYAP-GGLNPPHTHPRATEVIFVLEGELDVGFITTANKLFAKTVKKGEVFVFPRGLIHYQ 157

Query: 121 RNS-YDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIA 180
           +N+   +PAS    FNSQL GTQS+A  LF A+PA+ D VL  TFQIG   + +IKS  A
Sbjct: 158 KNNDKAKPASVISAFNSQLPGTQSIAATLFTATPAIPDHVLTTTFQIGTKEIEKIKSKFA 217

Query: 181 PTK 183
           P K
Sbjct: 218 PKK 219

[Close]

BLAST of MS018754 vs. TAIR 10
Match: AT1G09560.1 (germin-like protein 5 )

HSP 1 Score: 218.0 bits (554), Expect = 6.3e-57
Identity = 109/182 (59.89%), Postives = 134/182 (73.63%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           GIK+NGF CKD A V+++DFF  GLA     NN+ G   T A+V  IPGLNTLG+SL+R+
Sbjct: 39  GIKINGFPCKDAATVTSADFFSQGLAKPGLTNNTFGALVTGANVMTIPGLNTLGVSLSRI 98

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEVVFVL+G LDVGF+TTANKL+++++KKGDVF FPKGL+HFQ
Sbjct: 99  DYAP-GGLNPPHTHPRATEVVFVLEGTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQ 158

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+ D PAS    FNSQL GTQS+   LFG++P V D +LA  FQ     V  IKS   P
Sbjct: 159 KNNGDVPASVIAAFNSQLPGTQSLGATLFGSTPPVPDNILAQAFQTSPGTVKHIKSKFQP 218

Query: 181 TK 183
            K
Sbjct: 219 KK 219

[Close]

BLAST of MS018754 vs. TAIR 10
Match: AT1G02335.1 (germin-like protein subfamily 2 member 2 precursor )

HSP 1 Score: 214.5 bits (545), Expect = 6.9e-56
Identity = 108/182 (59.34%), Postives = 137/182 (75.27%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPAINNSLGFAATVADVNAIPGLNTLGLSLARV 60
           G K+NGF CK+  N++ SDFFFAG++    IN+++G A T A+V  IPGLNTL +SLAR+
Sbjct: 39  GTKLNGFPCKETLNITESDFFFAGISKPAVINSTMGSAVTGANVEKIPGLNTLSVSLARI 98

Query: 61  EYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHFQ 120
           +Y+P GGL PPH HPRATEVV+VL+G L+VGFITTANKL  KTIK G+VFVFP+GL+HFQ
Sbjct: 99  DYAP-GGLNPPHTHPRATEVVYVLEGELEVGFITTANKLFTKTIKIGEVFVFPRGLVHFQ 158

Query: 121 RNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIAP 180
           +N+   PAS    FNSQL GT SVA  LF A PA+ + VL  TFQ+G+ +V +IK  +A 
Sbjct: 159 KNNGKSPASVLSAFNSQLPGTASVAATLFAAEPALPEDVLTKTFQVGSKMVDKIKERLAT 218

Query: 181 TK 183
            K
Sbjct: 219 KK 219

[Close]

BLAST of MS018754 vs. TAIR 10
Match: AT3G05930.1 (germin-like protein 8 )

HSP 1 Score: 199.1 bits (505), Expect = 3.0e-51
Identity = 100/183 (54.64%), Postives = 131/183 (71.58%), Query Frame = 0


Query: 1   GIKVNGFACKDEANVSASDFFFAGLANAPA-INNSLGFAATVADVNAIPGLNTLGLSLAR 60
           G+KVNG+ CKD A V+  DF+F GLA A A  N+S+G A T A+V  +PGLNTLG+S++R
Sbjct: 38  GLKVNGYPCKDPAKVTPEDFYFIGLATAAATANSSMGSAVTGANVEKVPGLNTLGVSISR 97

Query: 61  VEYSPGGGLVPPHVHPRATEVVFVLDGVLDVGFITTANKLVAKTIKKGDVFVFPKGLLHF 120
           ++Y+P GGL PPH+HPRA+E +FVL+G L VGF+TT  KL++K + KGDVFVFPK LLHF
Sbjct: 98  IDYAP-GGLNPPHLHPRASEAIFVLEGRLFVGFLTTTGKLISKHVNKGDVFVFPKALLHF 157

Query: 121 QRNSYDRPASAFIVFNSQLFGTQSVAPALFGASPAVDDAVLAGTFQIGADVVAQIKSNIA 180
           Q+N    PAS    F+SQL GTQ V P+LFG++P + D +LA  F   A  + +IK    
Sbjct: 158 QQNPNKAPASVLAAFDSQLPGTQVVGPSLFGSNPPIPDDLLAKAFGAAAPEIQKIKGKFP 217

Query: 181 PTK 183
           P K
Sbjct: 218 PKK 219

The following BLAST results are available for this feature:

BLAST of MS018754 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146354.1	1.6e-75	82.51	germin-like protein subfamily 2 member 4 [Momordica charantia]	[more]
XP_038877903.1	1.1e-68	72.68	germin-like protein subfamily 2 member 4 [Benincasa hispida] >XP_038877904.1 ger...	[more]
KAA0050257.1	1.6e-67	72.13	germin-like protein subfamily 2 member 4 [Cucumis melo var. makuwa] >TYJ98151.1 ...	[more]
XP_008466192.1	2.1e-67	72.68	PREDICTED: germin-like protein subfamily 2 member 4 [Cucumis melo]	[more]
XP_008466206.1	2.4e-66	69.06	PREDICTED: germin-like protein subfamily 2 member 4 [Cucumis melo]	[more]

BLAST of MS018754 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M263	1.3e-59	63.39	Germin-like protein subfamily 2 member 4 OS=Arabidopsis thaliana OX=3702 GN=GLP1...	[more]
P94014	8.8e-56	59.89	Germin-like protein subfamily 2 member 1 OS=Arabidopsis thaliana OX=3702 GN=GLP4...	[more]
Q6I544	5.7e-55	61.54	Germin-like protein 5-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0277500...	[more]
Q9FZ27	9.7e-55	59.34	Germin-like protein subfamily 2 member 2 OS=Arabidopsis thaliana OX=3702 GN=At1g...	[more]
Q94EG3	4.5e-52	58.79	Nectarin-1 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NECI PE=1...	[more]

BLAST of MS018754 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXW0	7.9e-76	82.51	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111015585 PE=3 SV=1	[more]
A0A5A7U7A8	8.0e-68	72.13	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G...	[more]
A0A1S3CQM8	1.0e-67	72.68	Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503679 PE=3 SV=1	[more]
A0A1S3CQN9	1.2e-66	69.06	Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503687 PE=3 SV=1	[more]
A0A6J1JZY0	7.5e-66	70.49	Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111489804 PE=3 SV=1	[more]

BLAST of MS018754 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62020.2	9.3e-61	63.39	germin-like protein 10	[more]
AT3G62020.1	9.3e-61	63.39	germin-like protein 10	[more]
AT1G09560.1	6.3e-57	59.89	germin-like protein 5	[more]
AT1G02335.1	6.9e-56	59.34	germin-like protein subfamily 2 member 2 precursor	[more]
AT3G05930.1	3.0e-51	54.64	germin-like protein 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 102..122 score: 60.35 coord: 135..150 score: 46.37 coord: 72..92 score: 59.91
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 23..172 e-value: 3.4E-30 score: 116.3
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 25..169 e-value: 1.1E-41 score: 141.9
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 1..180 e-value: 1.7E-64 score: 218.9
None	No IPR available	PANTHER	PTHR31238:SF18	GERMIN-LIKE PROTEIN SUBFAMILY 2 MEMBER 4	coord: 1..180
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 1..180
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 4..178 e-value: 9.70575E-71 score: 210.53
IPR019780	Germin, manganese binding site	PROSITE	PS00725	GERMIN	coord: 67..80
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 15..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018754.1	MS018754.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0030145	manganese ion binding