MS018726 (gene) Bitter gourd (TR) v1

Overview
NameMS018726
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhosphatidylinositol 4-phosphate 5-kinase
Locationscaffold313: 1406299 .. 1409130 (+)
RNA-Seq ExpressionMS018726
SyntenyMS018726
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACCCGCCCAAATGTCCCACGTCGAGATGATCCTGCCCAACGGTGACTTCTACGCCGGCCGGTGGGTCCGGGACCTCCCCGACGGCCATGGAAAGTACTTGTGGACAGATGGGTGTATGTACGTCGGCGAATGGAGTGATGGGAAGCCGATGGGCACCGGAAAGTTCAGCTGGCCGTCCGGAGCAACCTACGAAGGCGATTTCAAGAGCGGCTTCATGGATGGGAAGGGAACGTTTACGGGTAGTTCGGGCGATACGTATAGAGGCAATTGGGTTATGAATTTGAAGCATGGCCAAGGGATTAAGAGCTTTGCCAATGGCGATTACTACGAGGGAGAATGGCGCCGCGGTTCGCAGAACGGGCATGGCCGGTATCAATGGCGGAATGGGAATCATTACATTGGGAAATGGAGGAATGGGAAGATCAATGGCAATGGGACGATGATTTGGAGGAATGGGAAGCGTTATGATGGGTATTGGGAGGATGGGTTTCCAAAAGGAAATGGGACATTCAGATGGTCAGATGGGAGTTTTTATGTTGGGATTTGGAGTAAGAATCCAAAAGAGCACTCTGGAACATATTATCCATCTGGGTCTACAGATAACAATTTGGATTGGGATCCTCAGGAGGTGTTTGATGTAGACTTGGTTGACTGCCTGATATACCCATGTGAAAAGGTTTCAATATTTCCTTCTGAGAAGCTGGTAAACAAGGGTGGATCTGAAGGGGAGTTGTTGCAGAGAAATTCAGTGGACAGCCATAGTTTTGAATCTGATGTTGGTTCTAAGGATGGGAGTGTCAGAAATGGAGTGGAAGGATCCATGGATGTTCCTACTCAAGATTCAGATGCCAAAGGAAATGGACAGCAGCAGTTGAGAGTACAATTGAAGGGACAGGGTGTGACAATATCTAAAGGCCATAAGAACTATGAACTTATGCTAAATCTTCAGTTGGGGATCAGGTATCAAAGGGGATCAACTTTATTTTGGTTCTTATTTTGTTTCCTATTTTCTTTATCTTTTGGTTTCTAGTTACTCCCATTTTGATCCTTGTTTTGGATTGCAACAATGTCCTCCAAGTGTGGTTTGCTCGTAGGAGTAGCTTTTGCAACCTTGAGAAATATCATCTCTCATGACATGTATTATTGCCATGTATTGTATACATCTTAGCGCTTAAATTTCAAACTGGTGATCTTTTAAACTGCTCTACTGGTTCTCTCCAGCCAATCAGTCTGAAACATGGATTTCAGGAAAAGTAAATTCATTCCTAGTGGTTTCGAAGTTTTGAAGTTTGTAAAATTTTCTAATCTTGCCAAGCTTCTATTGAAACTTTTCAACTTCCTAACTGAAAACCTGGGAACGCTTTGTTTTCCTCCAAAGTGAAGCAATGAAAGAGTTGATAAAATTTGCTGAGAAGTTTCAGAACAGGTTGCAAATTTATGAGATTTTATTCATGTCCCCGCTCCCACATTTTTCATTTTTATCTGGACAGACATTCTGCTGGAAGGCCTGCTCCTTCCTCTTCATTTGAATTGAAACCTTCAGCATTTGATCCAAAGGAGAAATTTTGGACAAAATTTCCACCAGAAGGATCTAAGTGCACTCCACCTCATCAATCCTGTGACTTCAGATGGAAGGATTACTGTCCTGTAGTATTCAGGTCAGCCATTAAATACTAACATGAATCTTCCTGCTAAACATCACTGCTGTATTTCTTTCCAATGATCTTTTGTATTGAGGGCAAGTTATCTAGTAAATGGTTTTATGTAACTTGTATAACTATATTATTGAAAACAGAAGGCTATTATCTTTTGTTCACCTATATCTATATACCGACACGATTTTTTGATGACCTAAGATGTTTGAGGTTGCCATCATTGGTAGCATAACTGAAGAACAATGCTTAAAACTCCTATATTGATCAAATAACTTTTCTTTGTAGAAAACATACATACATGTTCCACTTCACTATTACAAATGGAATGAAATAGTGCAGAGCCTCACGGATCATTCTATCCATATTTAGGACTCTTAGGAAGTTGTTCAACGTGGATCCAGCGGATTACATGCTATCAATATGTGGGAACGATGCCTTACGGGAGCTTTCCTCCCCCGGAAAAAGTGGAAGCTTCTTTTACTTAACCCACGATGACCGCTACATGATAAAAACCATGAGAAAGGCTGAAGTAAAAGTGAGTTTTCTTTGCCCTTGTTGGTTCAAATTCCTTGCTTCTTATGGTACACAAATTTAAACTAGAATCCTGTAAGAAGATCAGAGTATGGTTTCTCTTGTCCATTCCTCATCTTGTTTCCTCTGAGTTCTTTTTCTTTCCCTTGTCATACTTTCTTTCCAAATTTTGTGTCTGAGGCTGAAAAATGTGAATGTACAAGTTTTATATGAACATTACATGTCACTTTAATTGAAAGTTGTACCATTATAAATGCCAATAACACTCACCGTAAATTCCAGGTTCTCATACGGATGCTGCCAGCATACTATAAGCACGTTGAAGACTTCAAAAACACTCTGGTTACCAAATTCTATGGTCTTCATTGTGTGAAGTTAAGCAGTACTCAGAAAAAGGTAATGAGGGACATATTATAACATTAATTTGAAGCTTAACTTTCTTTGGATTCACATTCCTGCAATTACAGGTGCGATTTGTCATCATGGGGAACCTGTTTTGTTCGGAGCATTCAATTCACAGGCGATTTGACTTGAAAGGTTCCTCCCTTGGTCGTACAACCGATAAGCCAGAGTCAGAAATTGATGCAACCACAACCCTTAAAGATCTCGATCTAAATTATATATTTCGGTTGCAAAAGATTTGG

mRNA sequence

GAACCCGCCCAAATGTCCCACGTCGAGATGATCCTGCCCAACGGTGACTTCTACGCCGGCCGGTGGGTCCGGGACCTCCCCGACGGCCATGGAAAGTACTTGTGGACAGATGGGTGTATGTACGTCGGCGAATGGAGTGATGGGAAGCCGATGGGCACCGGAAAGTTCAGCTGGCCGTCCGGAGCAACCTACGAAGGCGATTTCAAGAGCGGCTTCATGGATGGGAAGGGAACGTTTACGGGTAGTTCGGGCGATACGTATAGAGGCAATTGGGTTATGAATTTGAAGCATGGCCAAGGGATTAAGAGCTTTGCCAATGGCGATTACTACGAGGGAGAATGGCGCCGCGGTTCGCAGAACGGGCATGGCCGGTATCAATGGCGGAATGGGAATCATTACATTGGGAAATGGAGGAATGGGAAGATCAATGGCAATGGGACGATGATTTGGAGGAATGGGAAGCGTTATGATGGGTATTGGGAGGATGGGTTTCCAAAAGGAAATGGGACATTCAGATGGTCAGATGGGAGTTTTTATGTTGGGATTTGGAGTAAGAATCCAAAAGAGCACTCTGGAACATATTATCCATCTGGGTCTACAGATAACAATTTGGATTGGGATCCTCAGGAGGTGTTTGATGTAGACTTGGTTGACTGCCTGATATACCCATGTGAAAAGGTTTCAATATTTCCTTCTGAGAAGCTGGTAAACAAGGGTGGATCTGAAGGGGAGTTGTTGCAGAGAAATTCAGTGGACAGCCATAGTTTTGAATCTGATGTTGGTTCTAAGGATGGGAGTGTCAGAAATGGAGTGGAAGGATCCATGGATGTTCCTACTCAAGATTCAGATGCCAAAGGAAATGGACAGCAGCAGTTGAGAGTACAATTGAAGGGACAGGGTGTGACAATATCTAAAGGCCATAAGAACTATGAACTTATGCTAAATCTTCAGTTGGGGATCAGACATTCTGCTGGAAGGCCTGCTCCTTCCTCTTCATTTGAATTGAAACCTTCAGCATTTGATCCAAAGGAGAAATTTTGGACAAAATTTCCACCAGAAGGATCTAAGTGCACTCCACCTCATCAATCCTGTGACTTCAGATGGAAGGATTACTGTCCTGTAGTATTCAGGACTCTTAGGAAGTTGTTCAACGTGGATCCAGCGGATTACATGCTATCAATATGTGGGAACGATGCCTTACGGGAGCTTTCCTCCCCCGGAAAAAGTGGAAGCTTCTTTTACTTAACCCACGATGACCGCTACATGATAAAAACCATGAGAAAGGCTGAAGTAAAAGTTCTCATACGGATGCTGCCAGCATACTATAAGCACGTTGAAGACTTCAAAAACACTCTGGTTACCAAATTCTATGGTCTTCATTGTGTGAAGTTAAGCAGTACTCAGAAAAAGGTGCGATTTGTCATCATGGGGAACCTGTTTTGTTCGGAGCATTCAATTCACAGGCGATTTGACTTGAAAGGTTCCTCCCTTGGTCGTACAACCGATAAGCCAGAGTCAGAAATTGATGCAACCACAACCCTTAAAGATCTCGATCTAAATTATATATTTCGGTTGCAAAAGATTTGG

Coding sequence (CDS)

GAACCCGCCCAAATGTCCCACGTCGAGATGATCCTGCCCAACGGTGACTTCTACGCCGGCCGGTGGGTCCGGGACCTCCCCGACGGCCATGGAAAGTACTTGTGGACAGATGGGTGTATGTACGTCGGCGAATGGAGTGATGGGAAGCCGATGGGCACCGGAAAGTTCAGCTGGCCGTCCGGAGCAACCTACGAAGGCGATTTCAAGAGCGGCTTCATGGATGGGAAGGGAACGTTTACGGGTAGTTCGGGCGATACGTATAGAGGCAATTGGGTTATGAATTTGAAGCATGGCCAAGGGATTAAGAGCTTTGCCAATGGCGATTACTACGAGGGAGAATGGCGCCGCGGTTCGCAGAACGGGCATGGCCGGTATCAATGGCGGAATGGGAATCATTACATTGGGAAATGGAGGAATGGGAAGATCAATGGCAATGGGACGATGATTTGGAGGAATGGGAAGCGTTATGATGGGTATTGGGAGGATGGGTTTCCAAAAGGAAATGGGACATTCAGATGGTCAGATGGGAGTTTTTATGTTGGGATTTGGAGTAAGAATCCAAAAGAGCACTCTGGAACATATTATCCATCTGGGTCTACAGATAACAATTTGGATTGGGATCCTCAGGAGGTGTTTGATGTAGACTTGGTTGACTGCCTGATATACCCATGTGAAAAGGTTTCAATATTTCCTTCTGAGAAGCTGGTAAACAAGGGTGGATCTGAAGGGGAGTTGTTGCAGAGAAATTCAGTGGACAGCCATAGTTTTGAATCTGATGTTGGTTCTAAGGATGGGAGTGTCAGAAATGGAGTGGAAGGATCCATGGATGTTCCTACTCAAGATTCAGATGCCAAAGGAAATGGACAGCAGCAGTTGAGAGTACAATTGAAGGGACAGGGTGTGACAATATCTAAAGGCCATAAGAACTATGAACTTATGCTAAATCTTCAGTTGGGGATCAGACATTCTGCTGGAAGGCCTGCTCCTTCCTCTTCATTTGAATTGAAACCTTCAGCATTTGATCCAAAGGAGAAATTTTGGACAAAATTTCCACCAGAAGGATCTAAGTGCACTCCACCTCATCAATCCTGTGACTTCAGATGGAAGGATTACTGTCCTGTAGTATTCAGGACTCTTAGGAAGTTGTTCAACGTGGATCCAGCGGATTACATGCTATCAATATGTGGGAACGATGCCTTACGGGAGCTTTCCTCCCCCGGAAAAAGTGGAAGCTTCTTTTACTTAACCCACGATGACCGCTACATGATAAAAACCATGAGAAAGGCTGAAGTAAAAGTTCTCATACGGATGCTGCCAGCATACTATAAGCACGTTGAAGACTTCAAAAACACTCTGGTTACCAAATTCTATGGTCTTCATTGTGTGAAGTTAAGCAGTACTCAGAAAAAGGTGCGATTTGTCATCATGGGGAACCTGTTTTGTTCGGAGCATTCAATTCACAGGCGATTTGACTTGAAAGGTTCCTCCCTTGGTCGTACAACCGATAAGCCAGAGTCAGAAATTGATGCAACCACAACCCTTAAAGATCTCGATCTAAATTATATATTTCGGTTGCAAAAGATTTGG

Protein sequence

EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW
Homology
BLAST of MS018726 vs. NCBI nr
Match: XP_022146326.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like [Momordica charantia])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPS 60
           EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPS
Sbjct: 44  EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPS 103

Query: 61  GATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQN 120
           GATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQN
Sbjct: 104 GATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQN 163

Query: 121 GHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYV 180
           GHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYV
Sbjct: 164 GHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYV 223

Query: 181 GIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGG 240
           GIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGG
Sbjct: 224 GIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGG 283

Query: 241 SEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQG 300
           SEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQG
Sbjct: 284 SEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQG 343

Query: 301 VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP 360
           VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP
Sbjct: 344 VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP 403

Query: 361 HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR 420
           HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR
Sbjct: 404 HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR 463

Query: 421 YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF 480
           YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF
Sbjct: 464 YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF 523

Query: 481 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW
Sbjct: 524 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 572

BLAST of MS018726 vs. NCBI nr
Match: XP_038875534.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1032.3 bits (2668), Expect = 1.4e-297
Identity = 483/528 (91.48%), Postives = 506/528 (95.83%), Query Frame = 0

Query: 3   AQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGA 62
           +Q+ H E IL NGDFYAGRWV+DLPDGHGKYLWTDGCMYVGEWS+GKP GTGKFSWPSGA
Sbjct: 47  SQIYHAEKILSNGDFYAGRWVKDLPDGHGKYLWTDGCMYVGEWSEGKPTGTGKFSWPSGA 106

Query: 63  TYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGH 122
           TYEGDFK+GFMDGKGT+TGSSGDTYRG WVMNLKHGQGIKSF NGD Y+GEWRRG QNGH
Sbjct: 107 TYEGDFKAGFMDGKGTYTGSSGDTYRGIWVMNLKHGQGIKSFVNGDCYDGEWRRGLQNGH 166

Query: 123 GRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 182
           GRYQWRNGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI
Sbjct: 167 GRYQWRNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 226

Query: 183 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSE 242
           WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDL DC IYPCEK+SI+PS+K+VN+GGSE
Sbjct: 227 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLKDCKIYPCEKISIYPSDKVVNQGGSE 286

Query: 243 GELLQRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGV 302
            ELLQR+SVDSHS ESDV G+KD SVRNG+EGSMDV  Q+SDAKGNGQQQ+RVQLKGQGV
Sbjct: 287 EELLQRDSVDSHSLESDVDGAKDESVRNGLEGSMDVQVQESDAKGNGQQQMRVQLKGQGV 346

Query: 303 TISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPH 362
           TISKGHKNYELMLNLQLGIRHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPH
Sbjct: 347 TISKGHKNYELMLNLQLGIRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPH 406

Query: 363 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRY 422
           QSCDFRWKDYCPVVFRTLRKLFNVDPADYM+SICGNDALRELSSPGKSGSFFYLTHDDRY
Sbjct: 407 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMISICGNDALRELSSPGKSGSFFYLTHDDRY 466

Query: 423 MIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLFC 482
           MIKTMRKAEVKVLIRMLPAYYKHV++FKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLFC
Sbjct: 467 MIKTMRKAEVKVLIRMLPAYYKHVQEFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLFC 526

Query: 483 SEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           SEH+IHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW
Sbjct: 527 SEHAIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 574

BLAST of MS018726 vs. NCBI nr
Match: XP_011655253.1 (phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis sativus] >XP_011655254.1 phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis sativus] >KGN51092.1 hypothetical protein Csa_008583 [Cucumis sativus])

HSP 1 Score: 1021.9 bits (2641), Expect = 1.9e-294
Identity = 477/529 (90.17%), Postives = 505/529 (95.46%), Query Frame = 0

Query: 3   AQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGA 62
           AQ  H E IL NGDFYAGRW++DLP+GHGKYLWTDGCMYVGEWS GKPMGTGKFSWPSGA
Sbjct: 47  AQTYHAEKILSNGDFYAGRWLKDLPNGHGKYLWTDGCMYVGEWSKGKPMGTGKFSWPSGA 106

Query: 63  TYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGH 122
           TYEGDFKSGFMDGKGTFTGSSGDTYRG WVMNLKHGQGIKSFANGDYY+GEWRRG QNGH
Sbjct: 107 TYEGDFKSGFMDGKGTFTGSSGDTYRGIWVMNLKHGQGIKSFANGDYYDGEWRRGLQNGH 166

Query: 123 GRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 182
           GRYQWRNGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI
Sbjct: 167 GRYQWRNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 226

Query: 183 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSE 242
           WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDL +C+IYPCEKVSI+PS+K+VN+ GSE
Sbjct: 227 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLNECMIYPCEKVSIYPSDKVVNQSGSE 286

Query: 243 GELLQRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGV 302
           GE LQR+S+DSHS ESDV G+KDGSVRN +EGSMD+  Q+SD+KGNGQQQ RVQLKGQGV
Sbjct: 287 GECLQRDSIDSHSLESDVDGAKDGSVRNELEGSMDIQVQESDSKGNGQQQTRVQLKGQGV 346

Query: 303 TISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPH 362
           TISKGHKNY+LMLNLQLG+RHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPH
Sbjct: 347 TISKGHKNYDLMLNLQLGVRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPH 406

Query: 363 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRY 422
           QSCDF+WKDYCPVVFRTLRKLFNVDPADYM+SICGNDALRELSSPGKSGSFFYLTHDDRY
Sbjct: 407 QSCDFKWKDYCPVVFRTLRKLFNVDPADYMISICGNDALRELSSPGKSGSFFYLTHDDRY 466

Query: 423 MIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLF 482
           MIKTMRKAEVKVLIRMLPAYYKHV+ FKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLF
Sbjct: 467 MIKTMRKAEVKVLIRMLPAYYKHVQGFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLF 526

Query: 483 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEH+IH+RFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW
Sbjct: 527 CSEHAIHKRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 575

BLAST of MS018726 vs. NCBI nr
Match: XP_008465842.1 (PREDICTED: phosphatidylinositol 4-phosphate 5-kinase 6-like [Cucumis melo] >XP_008465847.1 PREDICTED: phosphatidylinositol 4-phosphate 5-kinase 6-like [Cucumis melo] >XP_008465851.1 PREDICTED: phosphatidylinositol 4-phosphate 5-kinase 6-like [Cucumis melo] >KAA0050202.1 phosphatidylinositol 4-phosphate 5-kinase 6-like [Cucumis melo var. makuwa] >TYJ98209.1 phosphatidylinositol 4-phosphate 5-kinase 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.3e-294
Identity = 480/529 (90.74%), Postives = 502/529 (94.90%), Query Frame = 0

Query: 3   AQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGA 62
           AQ  H E IL NGDFYAGRW++DLP+GHGKYLWTDGCMYVGEWS GKPMGTGKFSWPSGA
Sbjct: 47  AQTYHAEKILSNGDFYAGRWLKDLPNGHGKYLWTDGCMYVGEWSKGKPMGTGKFSWPSGA 106

Query: 63  TYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGH 122
           TYEGDFKSGFMDGKGTFTGSSGDTYRG WVMNLKHGQGIKSFANGDYY+GEWRRG QNGH
Sbjct: 107 TYEGDFKSGFMDGKGTFTGSSGDTYRGIWVMNLKHGQGIKSFANGDYYDGEWRRGLQNGH 166

Query: 123 GRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 182
           GRYQWRNGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI
Sbjct: 167 GRYQWRNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 226

Query: 183 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSE 242
           WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDL DC+IYPCEKVSI+PS+K+VN+GGSE
Sbjct: 227 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLNDCMIYPCEKVSIYPSDKVVNQGGSE 286

Query: 243 GELLQRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGV 302
           GE LQ +S+DSHS ES V G KDGSVRNG+EGSMD   Q+SD+KGNGQQQ RVQLKGQGV
Sbjct: 287 GECLQSDSIDSHSLESYVDGVKDGSVRNGLEGSMDAHVQESDSKGNGQQQTRVQLKGQGV 346

Query: 303 TISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPH 362
           TISKGHKNY+LMLNLQLGIRHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPH
Sbjct: 347 TISKGHKNYDLMLNLQLGIRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPH 406

Query: 363 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRY 422
           QSCDFRWKDYCPVVFRTLRKLFNVDPADYM+SICGNDALRELSSPGKSGSFFYLTHDDRY
Sbjct: 407 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMISICGNDALRELSSPGKSGSFFYLTHDDRY 466

Query: 423 MIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLF 482
           MIKTMRKAEVKVLIRMLPAYYKHV+ FKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLF
Sbjct: 467 MIKTMRKAEVKVLIRMLPAYYKHVQGFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLF 526

Query: 483 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEH+IHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQ IW
Sbjct: 527 CSEHAIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQNIW 575

BLAST of MS018726 vs. NCBI nr
Match: XP_022993786.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 459/527 (87.10%), Postives = 489/527 (92.79%), Query Frame = 0

Query: 7   HVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEG 66
           H E IL NGDFYAG+W+ +LPDGHGKYLWTDGCMYVG+WS GKP G G+FSWPSGATYEG
Sbjct: 50  HAEKILSNGDFYAGQWLEELPDGHGKYLWTDGCMYVGDWSKGKPNGIGRFSWPSGATYEG 109

Query: 67  DFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQ 126
           DFK GFMDGKGT+TGSSGDT+RGNWVMNLKHGQGIKSF NGDYY+GEWRRGSQNGHGRYQ
Sbjct: 110 DFKVGFMDGKGTYTGSSGDTFRGNWVMNLKHGQGIKSFLNGDYYDGEWRRGSQNGHGRYQ 169

Query: 127 WRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 186
           W+NGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN
Sbjct: 170 WQNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 229

Query: 187 PKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEGELL 246
           PKEHSGTYYPSGSTDNNLDWDP EVFDVDL +C IYPCE++S++PSEK V++ G+  +LL
Sbjct: 230 PKEHSGTYYPSGSTDNNLDWDPHEVFDVDLSECKIYPCERISLYPSEKSVHQAGA--DLL 289

Query: 247 QRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNG-QQQLRVQLK-GQGVTI 306
           QR+S+DSHSFESDV G KD  VR  +E S+D+  QD DAKG+  QQQ RVQLK GQGVTI
Sbjct: 290 QRDSIDSHSFESDVDGGKDDIVRAELERSIDIHVQDLDAKGDAQQQQTRVQLKGGQGVTI 349

Query: 307 SKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPHQS 366
           SKGHKNYELMLNLQLGIRHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPHQS
Sbjct: 350 SKGHKNYELMLNLQLGIRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPHQS 409

Query: 367 CDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMI 426
           CDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMI
Sbjct: 410 CDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMI 469

Query: 427 KTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLFCS 486
           KTMRKAEVKVLIRMLPAYYKHV+DFKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLFCS
Sbjct: 470 KTMRKAEVKVLIRMLPAYYKHVQDFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLFCS 529

Query: 487 EHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           EH+IHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQ  W
Sbjct: 530 EHAIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQNAW 574

BLAST of MS018726 vs. ExPASy Swiss-Prot
Match: Q9SFB8 (Phosphatidylinositol 4-phosphate 5-kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PIP5K6 PE=2 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.4e-223
Identity = 372/531 (70.06%), Postives = 430/531 (80.98%), Query Frame = 0

Query: 7   HVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEG 66
           H E  LP+GDFY G+W  +LP GHGKYLWTDGCMYVG+W  GK MG G+FSWPSGATYEG
Sbjct: 21  HAEKALPSGDFYTGQWRDNLPHGHGKYLWTDGCMYVGDWHRGKTMGKGRFSWPSGATYEG 80

Query: 67  DFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQ 126
           DFK+G+MDGKGT+  SSGD YRG+WVMNL+HGQG KS+ NGD Y+GEWRRG Q+GHGRYQ
Sbjct: 81  DFKNGYMDGKGTYIDSSGDLYRGSWVMNLRHGQGTKSYVNGDCYDGEWRRGLQDGHGRYQ 140

Query: 127 WRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 186
           W+N NHYIG+W+NG +NGNGTMIW NG RYDG WEDG PKGNGTFRWSDGSFYVG+WSK+
Sbjct: 141 WKNENHYIGQWKNGLMNGNGTMIWSNGNRYDGSWEDGAPKGNGTFRWSDGSFYVGVWSKD 200

Query: 187 PKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKL-VNKGGSEGEL 246
           PKE +GTYYPS S+  N DW PQ+VF VDL +C++  C+++ + PS+K+ V  G SE   
Sbjct: 201 PKEQNGTYYPSTSS-GNFDWQPQQVFYVDLSECVVCTCQRIPVLPSQKMPVWYGASEQSS 260

Query: 247 LQRNSVDS-----HSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQ-LKGQ 306
               + +S      S +  V + +  +R+   G + V   ++++  +    LR+Q  K Q
Sbjct: 261 SGNRTKNSERPRRRSVDGRVSNGEMELRSNGSGYLQV-DDNAESTRSSLGPLRIQPAKKQ 320

Query: 307 GVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTP 366
           G TISKGHKNYELMLNLQLGIRHS GRPAP++S +LK SAFDPKEK WTKFP EGSK TP
Sbjct: 321 GQTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKASAFDPKEKLWTKFPSEGSKYTP 380

Query: 367 PHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDD 426
           PHQSC+F+WKDYCPVVFRTLRKLF+VD ADYMLSICGNDALRELSSPGKSGSFFYLT+DD
Sbjct: 381 PHQSCEFKWKDYCPVVFRTLRKLFSVDAADYMLSICGNDALRELSSPGKSGSFFYLTNDD 440

Query: 427 RYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSST-QKKVRFVIMGN 486
           RYMIKTM+KAE KVLIRMLPAYY HV   +NTLVTKF+GLHCVKL+ T QKKVRFVIMGN
Sbjct: 441 RYMIKTMKKAETKVLIRMLPAYYNHVRACENTLVTKFFGLHCVKLTGTAQKKVRFVIMGN 500

Query: 487 LFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           LFC+ HSIHRRFDLKGSS GR T KPESEID  TTLKDLDLN+ FRLQK W
Sbjct: 501 LFCTGHSIHRRFDLKGSSHGRLTTKPESEIDPNTTLKDLDLNFAFRLQKNW 549

BLAST of MS018726 vs. ExPASy Swiss-Prot
Match: Q9SLG9 (Phosphatidylinositol 4-phosphate 5-kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PIP5K5 PE=2 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 1.6e-206
Identity = 345/542 (63.65%), Postives = 424/542 (78.23%), Query Frame = 0

Query: 7   HVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEG 66
           H E +LPNGD+Y G+W    P GHGKYLWTDGCMY+G+W +GK MG GKF WPSGATYEG
Sbjct: 64  HAERVLPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGATYEG 123

Query: 67  DFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQ 126
           +FKSG+MDG GT+TG SGD Y+G WVMNLKHG G+KSFANGD Y+GEWRRG Q G G+YQ
Sbjct: 124 EFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQGKYQ 183

Query: 127 WRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 186
           W +G++YIG+W+NG I G G+ +W NG RYDG+W++GFP+GNGTF+W +GSFYVG WSK+
Sbjct: 184 WSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGHWSKD 243

Query: 187 PKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEG--E 246
           P+E +GTYYPSG+ + NL+WDP+++F+ +L +  I   E+V   PS+K ++   S    E
Sbjct: 244 PEEMNGTYYPSGN-EGNLEWDPKDLFN-NLSEYTICSGERVPTLPSQKKLSVWNSSKRIE 303

Query: 247 LLQRNSVD-------SHSFE--SDVGSKDGSVRNGVEGSMDVPTQDSDAKG-----NGQQ 306
             +R SVD         +FE  +  G++ G     +   +D      DA+G     +   
Sbjct: 304 KPRRTSVDGRVSVGVDRAFEKMNMWGNEIGEGGADMRKELDAELMRLDAEGLQSLKSSPV 363

Query: 307 QLRVQLKG--QGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWT 366
            +++   G  QG TISKGH+NYELMLNLQLGIRHS GR AP++S +LKPSAFDPK+K W 
Sbjct: 364 PMKLPKAGRKQGETISKGHRNYELMLNLQLGIRHSVGRQAPAASLDLKPSAFDPKDKIWR 423

Query: 367 KFPPEGSKCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGK 426
           +FP EG+K TPPHQS +F+WKDYCP+VFR+LRKLF VDPADYMLSICGNDALRELSSPGK
Sbjct: 424 RFPREGTKYTPPHQSTEFKWKDYCPLVFRSLRKLFKVDPADYMLSICGNDALRELSSPGK 483

Query: 427 SGSFFYLTHDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSS-T 486
           SGSFFYLT+DDRYMIKTM+K+E KVL+ ML AYY HV  F+N+LV +F+GLHCVKL+  T
Sbjct: 484 SGSFFYLTNDDRYMIKTMKKSETKVLLGMLAAYYNHVRAFENSLVIRFFGLHCVKLNGPT 543

Query: 487 QKKVRFVIMGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQK 530
           QKKVRFVIMGNLFCS++S+HRRFDLKGSSLGRTTDKPESEID+ T LKDLDLN+IFRLQK
Sbjct: 544 QKKVRFVIMGNLFCSKYSVHRRFDLKGSSLGRTTDKPESEIDSNTILKDLDLNFIFRLQK 603

BLAST of MS018726 vs. ExPASy Swiss-Prot
Match: Q9M1K2 (Phosphatidylinositol 4-phosphate 5-kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PIP5K4 PE=4 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 7.8e-206
Identity = 349/550 (63.45%), Postives = 420/550 (76.36%), Query Frame = 0

Query: 4   QMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGAT 63
           ++ H E ILPNGD+Y G+W    P GHGKYLWTDGCMY+G+W +GK MG GKF WPSGAT
Sbjct: 63  ELYHAERILPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGRGKFGWPSGAT 122

Query: 64  YEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHG 123
           YEG+FKSG+MDG G +TG SGDTY+G WVMNLKHG GIK FANGD Y+GEWRRG Q   G
Sbjct: 123 YEGEFKSGYMDGVGLYTGPSGDTYKGQWVMNLKHGHGIKRFANGDVYDGEWRRGLQEAQG 182

Query: 124 RYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIW 183
           +YQWR+G++Y+G+W+N  I G GT IW +G RYDG+W+DGFP+GNGTF+W+DGSFYVG W
Sbjct: 183 KYQWRDGSYYMGEWKNATICGKGTFIWTDGNRYDGFWDDGFPRGNGTFKWADGSFYVGHW 242

Query: 184 SKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEG 243
           S +P+E +GTYYPSG  D + +WDP++VF  +L +  I   E+V + PS+K ++   S  
Sbjct: 243 SNDPEEMNGTYYPSGD-DGSPEWDPKDVF-TNLSEYKICSGERVPVLPSQKKLSVWNSSK 302

Query: 244 --ELLQRNSVD-------SHSFE------SDVGSKDGSVRNGVEGSMDVPTQDSDAKGNG 303
             E  +R SVD         +FE      ++ G     + +     +D      +A+G  
Sbjct: 303 RVEKPRRMSVDGRVSVGVDRAFEKMNMWGTESGEGAADIDSTTRRDLDAEMMRLEAEGFI 362

Query: 304 QQ------QLRVQLKG--QGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAF 363
           Q        +R+   G  QG TISKGH+NYELMLNLQLGIRH+ G+ AP  S +LK SAF
Sbjct: 363 QSLRPSPAPMRLPRAGRKQGETISKGHRNYELMLNLQLGIRHAVGKQAPVVSLDLKHSAF 422

Query: 364 DPKEKFWTKFPPEGSKCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDAL 423
           DPKEK WT+FPPEG+K TPPHQS +F+WKDYCP+VFR+LRKLF VDPADYMLSICGNDAL
Sbjct: 423 DPKEKVWTRFPPEGTKYTPPHQSSEFKWKDYCPLVFRSLRKLFKVDPADYMLSICGNDAL 482

Query: 424 RELSSPGKSGSFFYLTHDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLH 483
           RELSSPGKSGSFFYLT+DDRYMIKTM+K+E KVL+RML AYY HV  F+NTLV +FYGLH
Sbjct: 483 RELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLRMLAAYYNHVRAFENTLVIRFYGLH 542

Query: 484 CVKLSS-TQKKVRFVIMGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDL 530
           CVKL+   QKKVRFVIMGNLFCSE+SIHRRFDLKGSSLGRTTDKPESEI++ T LKDLDL
Sbjct: 543 CVKLTGPIQKKVRFVIMGNLFCSEYSIHRRFDLKGSSLGRTTDKPESEINSNTILKDLDL 602

BLAST of MS018726 vs. ExPASy Swiss-Prot
Match: Q56YP2 (Phosphatidylinositol 4-phosphate 5-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PIP5K1 PE=1 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.9e-167
Identity = 304/534 (56.93%), Postives = 366/534 (68.54%), Query Frame = 0

Query: 2   PAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSG 61
           P  +  VE  LPNGD Y G +    P G GKYLW DGCMY G+W  GK  G GKFSWPSG
Sbjct: 65  PTPLVDVEKPLPNGDLYIGSFSGGFPHGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSG 124

Query: 62  ATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNG 121
           ATYEG+FKSG M+G GTFTG+ GDTYRG WV + KHG G K +ANGD+YEG WRR  Q+G
Sbjct: 125 ATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDG 184

Query: 122 HGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVG 181
            GRY WRNGN Y G+WR+G I+G G ++W NG RY+G WE+G PKGNG F WSDGS  VG
Sbjct: 185 RGRYVWRNGNQYTGEWRSGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVG 244

Query: 182 IWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGS 241
            W+++    S   + +G   N+L    ++   VD                     +  GS
Sbjct: 245 AWNESNIMRS---FFNGVEKNDLIVGNRKRSSVD---------------------SGAGS 304

Query: 242 EGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSM---DVPTQDSDAKGNGQQQ---LRVQ 301
            G       +     + + G     + + VE SM   D  + D D     ++       +
Sbjct: 305 LGGEKVFPRICIWESDGEAGDITCDIIDNVEASMIYRDRISVDRDGFRQFKKNPCWFNGE 364

Query: 302 LKGQGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGS 361
            K  G TISKGHK Y+LMLNLQLGIR+S G+ A S   +LK + FDPKEKFWT+FPPEG+
Sbjct: 365 AKKPGQTISKGHKKYDLMLNLQLGIRYSVGKHA-SIVRDLKQTDFDPKEKFWTRFPPEGT 424

Query: 362 KCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYL 421
           K TPPHQS DFRWKDYCP+VFR LR+LF VDPA YML+ICGNDALRELSSPGKSGSFFYL
Sbjct: 425 KTTPPHQSVDFRWKDYCPLVFRRLRELFQVDPAKYMLAICGNDALRELSSPGKSGSFFYL 484

Query: 422 THDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVI 481
           T DDR+MIKT++K+EVKVL+RMLP+YYKHV  ++N+LVT+FYG+HCVK    Q K RF++
Sbjct: 485 TQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENSLVTRFYGVHCVKPVGGQ-KTRFIV 544

Query: 482 MGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           MGNLFCSE+ I RRFDLKGSS GR+T KPE EID TTTLKDLDLN+ FRLQ+ W
Sbjct: 545 MGNLFCSEYRIQRRFDLKGSSHGRSTAKPEGEIDETTTLKDLDLNFSFRLQRNW 572

BLAST of MS018726 vs. ExPASy Swiss-Prot
Match: Q8L796 (Phosphatidylinositol 4-phosphate 5-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PIP5K2 PE=1 SV=2)

HSP 1 Score: 581.6 bits (1498), Expect = 8.8e-165
Identity = 305/529 (57.66%), Postives = 361/529 (68.24%), Query Frame = 0

Query: 8   VEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEGD 67
           VE  LPNGD Y G +    P+G GKYLW DGCMY GEW  GK  G GKFSWPSGATYEG+
Sbjct: 72  VEKPLPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGE 131

Query: 68  FKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQW 127
           FKSG M+G GTF G  GDTYRG+WV + K G G K +ANGDYYEG WRR  Q+G GRY W
Sbjct: 132 FKSGRMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVW 191

Query: 128 RNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKNP 187
            NGN Y G+WRNG I G G + W NG RY+G WE+G PKG+G F W+DGS ++G W+++ 
Sbjct: 192 MNGNQYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSWNES- 251

Query: 188 KEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEGELLQ 247
                         +NL    +  FD    + LI    K S   S      G   GE + 
Sbjct: 252 --------------SNL---MRNFFDGIEKNELIVATRKRSSVDS----GAGSLTGEKIF 311

Query: 248 RNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRV-------QLKGQG 307
                  S + + G     + + VE S+    + S  K +G +Q R        + K  G
Sbjct: 312 PRICIWES-DGEAGDITCDIVDNVEASVIYRDRISIDK-DGFRQFRKNPCCFSGEAKKPG 371

Query: 308 VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP 367
            TISKGHK Y+LMLNLQ GIR+S G+ A S   +LK S FDP EKFWT+FPPEGSK TPP
Sbjct: 372 ETISKGHKKYDLMLNLQHGIRYSVGKHA-SVVRDLKQSDFDPSEKFWTRFPPEGSKTTPP 431

Query: 368 HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR 427
           H S DFRWKDYCP+VFR LR+LF VDPADYML+ICGNDALRELSSPGKSGSFFYLT DDR
Sbjct: 432 HLSVDFRWKDYCPLVFRRLRELFTVDPADYMLAICGNDALRELSSPGKSGSFFYLTQDDR 491

Query: 428 YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF 487
           +MIKT++K+EVKVL+RMLP+YYKHV  ++NTLVT+FYG+HC+K    Q K RF++MGNLF
Sbjct: 492 FMIKTVKKSEVKVLLRMLPSYYKHVCQYENTLVTRFYGVHCIKPVGGQ-KTRFIVMGNLF 551

Query: 488 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSE+ I RRFDLKGSS GR T KPE EID TTTLKDLDLN+ FRLQ+ W
Sbjct: 552 CSEYRIQRRFDLKGSSHGRYTSKPEGEIDETTTLKDLDLNFAFRLQRNW 574

BLAST of MS018726 vs. ExPASy TrEMBL
Match: A0A6J1CZ15 (Phosphatidylinositol 4-phosphate 5-kinase OS=Momordica charantia OX=3673 GN=LOC111015564 PE=4 SV=1)

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPS 60
           EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPS
Sbjct: 44  EPAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPS 103

Query: 61  GATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQN 120
           GATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQN
Sbjct: 104 GATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQN 163

Query: 121 GHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYV 180
           GHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYV
Sbjct: 164 GHGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYV 223

Query: 181 GIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGG 240
           GIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGG
Sbjct: 224 GIWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGG 283

Query: 241 SEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQG 300
           SEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQG
Sbjct: 284 SEGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQG 343

Query: 301 VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP 360
           VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP
Sbjct: 344 VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP 403

Query: 361 HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR 420
           HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR
Sbjct: 404 HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR 463

Query: 421 YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF 480
           YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF
Sbjct: 464 YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF 523

Query: 481 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW
Sbjct: 524 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 572

BLAST of MS018726 vs. ExPASy TrEMBL
Match: A0A0A0KRS0 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G435070 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 9.4e-295
Identity = 477/529 (90.17%), Postives = 505/529 (95.46%), Query Frame = 0

Query: 3   AQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGA 62
           AQ  H E IL NGDFYAGRW++DLP+GHGKYLWTDGCMYVGEWS GKPMGTGKFSWPSGA
Sbjct: 47  AQTYHAEKILSNGDFYAGRWLKDLPNGHGKYLWTDGCMYVGEWSKGKPMGTGKFSWPSGA 106

Query: 63  TYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGH 122
           TYEGDFKSGFMDGKGTFTGSSGDTYRG WVMNLKHGQGIKSFANGDYY+GEWRRG QNGH
Sbjct: 107 TYEGDFKSGFMDGKGTFTGSSGDTYRGIWVMNLKHGQGIKSFANGDYYDGEWRRGLQNGH 166

Query: 123 GRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 182
           GRYQWRNGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI
Sbjct: 167 GRYQWRNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 226

Query: 183 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSE 242
           WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDL +C+IYPCEKVSI+PS+K+VN+ GSE
Sbjct: 227 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLNECMIYPCEKVSIYPSDKVVNQSGSE 286

Query: 243 GELLQRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGV 302
           GE LQR+S+DSHS ESDV G+KDGSVRN +EGSMD+  Q+SD+KGNGQQQ RVQLKGQGV
Sbjct: 287 GECLQRDSIDSHSLESDVDGAKDGSVRNELEGSMDIQVQESDSKGNGQQQTRVQLKGQGV 346

Query: 303 TISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPH 362
           TISKGHKNY+LMLNLQLG+RHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPH
Sbjct: 347 TISKGHKNYDLMLNLQLGVRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPH 406

Query: 363 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRY 422
           QSCDF+WKDYCPVVFRTLRKLFNVDPADYM+SICGNDALRELSSPGKSGSFFYLTHDDRY
Sbjct: 407 QSCDFKWKDYCPVVFRTLRKLFNVDPADYMISICGNDALRELSSPGKSGSFFYLTHDDRY 466

Query: 423 MIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLF 482
           MIKTMRKAEVKVLIRMLPAYYKHV+ FKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLF
Sbjct: 467 MIKTMRKAEVKVLIRMLPAYYKHVQGFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLF 526

Query: 483 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEH+IH+RFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW
Sbjct: 527 CSEHAIHKRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 575

BLAST of MS018726 vs. ExPASy TrEMBL
Match: A0A1S3CR89 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC103503435 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.6e-294
Identity = 480/529 (90.74%), Postives = 502/529 (94.90%), Query Frame = 0

Query: 3   AQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGA 62
           AQ  H E IL NGDFYAGRW++DLP+GHGKYLWTDGCMYVGEWS GKPMGTGKFSWPSGA
Sbjct: 47  AQTYHAEKILSNGDFYAGRWLKDLPNGHGKYLWTDGCMYVGEWSKGKPMGTGKFSWPSGA 106

Query: 63  TYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGH 122
           TYEGDFKSGFMDGKGTFTGSSGDTYRG WVMNLKHGQGIKSFANGDYY+GEWRRG QNGH
Sbjct: 107 TYEGDFKSGFMDGKGTFTGSSGDTYRGIWVMNLKHGQGIKSFANGDYYDGEWRRGLQNGH 166

Query: 123 GRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 182
           GRYQWRNGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI
Sbjct: 167 GRYQWRNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 226

Query: 183 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSE 242
           WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDL DC+IYPCEKVSI+PS+K+VN+GGSE
Sbjct: 227 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLNDCMIYPCEKVSIYPSDKVVNQGGSE 286

Query: 243 GELLQRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGV 302
           GE LQ +S+DSHS ES V G KDGSVRNG+EGSMD   Q+SD+KGNGQQQ RVQLKGQGV
Sbjct: 287 GECLQSDSIDSHSLESYVDGVKDGSVRNGLEGSMDAHVQESDSKGNGQQQTRVQLKGQGV 346

Query: 303 TISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPH 362
           TISKGHKNY+LMLNLQLGIRHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPH
Sbjct: 347 TISKGHKNYDLMLNLQLGIRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPH 406

Query: 363 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRY 422
           QSCDFRWKDYCPVVFRTLRKLFNVDPADYM+SICGNDALRELSSPGKSGSFFYLTHDDRY
Sbjct: 407 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMISICGNDALRELSSPGKSGSFFYLTHDDRY 466

Query: 423 MIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLF 482
           MIKTMRKAEVKVLIRMLPAYYKHV+ FKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLF
Sbjct: 467 MIKTMRKAEVKVLIRMLPAYYKHVQGFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLF 526

Query: 483 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEH+IHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQ IW
Sbjct: 527 CSEHAIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQNIW 575

BLAST of MS018726 vs. ExPASy TrEMBL
Match: A0A5D3BGP0 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G001030 PE=4 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.6e-294
Identity = 480/529 (90.74%), Postives = 502/529 (94.90%), Query Frame = 0

Query: 3   AQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGA 62
           AQ  H E IL NGDFYAGRW++DLP+GHGKYLWTDGCMYVGEWS GKPMGTGKFSWPSGA
Sbjct: 47  AQTYHAEKILSNGDFYAGRWLKDLPNGHGKYLWTDGCMYVGEWSKGKPMGTGKFSWPSGA 106

Query: 63  TYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGH 122
           TYEGDFKSGFMDGKGTFTGSSGDTYRG WVMNLKHGQGIKSFANGDYY+GEWRRG QNGH
Sbjct: 107 TYEGDFKSGFMDGKGTFTGSSGDTYRGIWVMNLKHGQGIKSFANGDYYDGEWRRGLQNGH 166

Query: 123 GRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 182
           GRYQWRNGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI
Sbjct: 167 GRYQWRNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGI 226

Query: 183 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSE 242
           WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDL DC+IYPCEKVSI+PS+K+VN+GGSE
Sbjct: 227 WSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLNDCMIYPCEKVSIYPSDKVVNQGGSE 286

Query: 243 GELLQRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQLKGQGV 302
           GE LQ +S+DSHS ES V G KDGSVRNG+EGSMD   Q+SD+KGNGQQQ RVQLKGQGV
Sbjct: 287 GECLQSDSIDSHSLESYVDGVKDGSVRNGLEGSMDAHVQESDSKGNGQQQTRVQLKGQGV 346

Query: 303 TISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPH 362
           TISKGHKNY+LMLNLQLGIRHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPH
Sbjct: 347 TISKGHKNYDLMLNLQLGIRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPH 406

Query: 363 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRY 422
           QSCDFRWKDYCPVVFRTLRKLFNVDPADYM+SICGNDALRELSSPGKSGSFFYLTHDDRY
Sbjct: 407 QSCDFRWKDYCPVVFRTLRKLFNVDPADYMISICGNDALRELSSPGKSGSFFYLTHDDRY 466

Query: 423 MIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLF 482
           MIKTMRKAEVKVLIRMLPAYYKHV+ FKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLF
Sbjct: 467 MIKTMRKAEVKVLIRMLPAYYKHVQGFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLF 526

Query: 483 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSEH+IHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQ IW
Sbjct: 527 CSEHAIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQNIW 575

BLAST of MS018726 vs. ExPASy TrEMBL
Match: A0A6J1JZH2 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111489692 PE=4 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.5e-279
Identity = 459/527 (87.10%), Postives = 489/527 (92.79%), Query Frame = 0

Query: 7   HVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEG 66
           H E IL NGDFYAG+W+ +LPDGHGKYLWTDGCMYVG+WS GKP G G+FSWPSGATYEG
Sbjct: 50  HAEKILSNGDFYAGQWLEELPDGHGKYLWTDGCMYVGDWSKGKPNGIGRFSWPSGATYEG 109

Query: 67  DFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQ 126
           DFK GFMDGKGT+TGSSGDT+RGNWVMNLKHGQGIKSF NGDYY+GEWRRGSQNGHGRYQ
Sbjct: 110 DFKVGFMDGKGTYTGSSGDTFRGNWVMNLKHGQGIKSFLNGDYYDGEWRRGSQNGHGRYQ 169

Query: 127 WRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 186
           W+NGNHYIGKWRNGKINGNGTMIW NGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN
Sbjct: 170 WQNGNHYIGKWRNGKINGNGTMIWSNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 229

Query: 187 PKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEGELL 246
           PKEHSGTYYPSGSTDNNLDWDP EVFDVDL +C IYPCE++S++PSEK V++ G+  +LL
Sbjct: 230 PKEHSGTYYPSGSTDNNLDWDPHEVFDVDLSECKIYPCERISLYPSEKSVHQAGA--DLL 289

Query: 247 QRNSVDSHSFESDV-GSKDGSVRNGVEGSMDVPTQDSDAKGNG-QQQLRVQLK-GQGVTI 306
           QR+S+DSHSFESDV G KD  VR  +E S+D+  QD DAKG+  QQQ RVQLK GQGVTI
Sbjct: 290 QRDSIDSHSFESDVDGGKDDIVRAELERSIDIHVQDLDAKGDAQQQQTRVQLKGGQGVTI 349

Query: 307 SKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPPHQS 366
           SKGHKNYELMLNLQLGIRHS GRPAP+SSF+LK SAFDPKEK WTKFPPEGSK TPPHQS
Sbjct: 350 SKGHKNYELMLNLQLGIRHSVGRPAPASSFDLKASAFDPKEKIWTKFPPEGSKYTPPHQS 409

Query: 367 CDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMI 426
           CDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMI
Sbjct: 410 CDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMI 469

Query: 427 KTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLS-STQKKVRFVIMGNLFCS 486
           KTMRKAEVKVLIRMLPAYYKHV+DFKNTLVTKFYGLHCVKL+ +TQKKVRFVIMGNLFCS
Sbjct: 470 KTMRKAEVKVLIRMLPAYYKHVQDFKNTLVTKFYGLHCVKLTGTTQKKVRFVIMGNLFCS 529

Query: 487 EHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           EH+IHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQ  W
Sbjct: 530 EHAIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQNAW 574

BLAST of MS018726 vs. TAIR 10
Match: AT3G07960.1 (Phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 775.0 bits (2000), Expect = 3.8e-224
Identity = 372/531 (70.06%), Postives = 430/531 (80.98%), Query Frame = 0

Query: 7   HVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEG 66
           H E  LP+GDFY G+W  +LP GHGKYLWTDGCMYVG+W  GK MG G+FSWPSGATYEG
Sbjct: 21  HAEKALPSGDFYTGQWRDNLPHGHGKYLWTDGCMYVGDWHRGKTMGKGRFSWPSGATYEG 80

Query: 67  DFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQ 126
           DFK+G+MDGKGT+  SSGD YRG+WVMNL+HGQG KS+ NGD Y+GEWRRG Q+GHGRYQ
Sbjct: 81  DFKNGYMDGKGTYIDSSGDLYRGSWVMNLRHGQGTKSYVNGDCYDGEWRRGLQDGHGRYQ 140

Query: 127 WRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 186
           W+N NHYIG+W+NG +NGNGTMIW NG RYDG WEDG PKGNGTFRWSDGSFYVG+WSK+
Sbjct: 141 WKNENHYIGQWKNGLMNGNGTMIWSNGNRYDGSWEDGAPKGNGTFRWSDGSFYVGVWSKD 200

Query: 187 PKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKL-VNKGGSEGEL 246
           PKE +GTYYPS S+  N DW PQ+VF VDL +C++  C+++ + PS+K+ V  G SE   
Sbjct: 201 PKEQNGTYYPSTSS-GNFDWQPQQVFYVDLSECVVCTCQRIPVLPSQKMPVWYGASEQSS 260

Query: 247 LQRNSVDS-----HSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRVQ-LKGQ 306
               + +S      S +  V + +  +R+   G + V   ++++  +    LR+Q  K Q
Sbjct: 261 SGNRTKNSERPRRRSVDGRVSNGEMELRSNGSGYLQV-DDNAESTRSSLGPLRIQPAKKQ 320

Query: 307 GVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTP 366
           G TISKGHKNYELMLNLQLGIRHS GRPAP++S +LK SAFDPKEK WTKFP EGSK TP
Sbjct: 321 GQTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKASAFDPKEKLWTKFPSEGSKYTP 380

Query: 367 PHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDD 426
           PHQSC+F+WKDYCPVVFRTLRKLF+VD ADYMLSICGNDALRELSSPGKSGSFFYLT+DD
Sbjct: 381 PHQSCEFKWKDYCPVVFRTLRKLFSVDAADYMLSICGNDALRELSSPGKSGSFFYLTNDD 440

Query: 427 RYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSST-QKKVRFVIMGN 486
           RYMIKTM+KAE KVLIRMLPAYY HV   +NTLVTKF+GLHCVKL+ T QKKVRFVIMGN
Sbjct: 441 RYMIKTMKKAETKVLIRMLPAYYNHVRACENTLVTKFFGLHCVKLTGTAQKKVRFVIMGN 500

Query: 487 LFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           LFC+ HSIHRRFDLKGSS GR T KPESEID  TTLKDLDLN+ FRLQK W
Sbjct: 501 LFCTGHSIHRRFDLKGSSHGRLTTKPESEIDPNTTLKDLDLNFAFRLQKNW 549

BLAST of MS018726 vs. TAIR 10
Match: AT2G41210.1 (phosphatidylinositol- 4-phosphate 5-kinase 5 )

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-207
Identity = 345/542 (63.65%), Postives = 424/542 (78.23%), Query Frame = 0

Query: 7   HVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEG 66
           H E +LPNGD+Y G+W    P GHGKYLWTDGCMY+G+W +GK MG GKF WPSGATYEG
Sbjct: 64  HAERVLPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGATYEG 123

Query: 67  DFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQ 126
           +FKSG+MDG GT+TG SGD Y+G WVMNLKHG G+KSFANGD Y+GEWRRG Q G G+YQ
Sbjct: 124 EFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQGKYQ 183

Query: 127 WRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKN 186
           W +G++YIG+W+NG I G G+ +W NG RYDG+W++GFP+GNGTF+W +GSFYVG WSK+
Sbjct: 184 WSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGHWSKD 243

Query: 187 PKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEG--E 246
           P+E +GTYYPSG+ + NL+WDP+++F+ +L +  I   E+V   PS+K ++   S    E
Sbjct: 244 PEEMNGTYYPSGN-EGNLEWDPKDLFN-NLSEYTICSGERVPTLPSQKKLSVWNSSKRIE 303

Query: 247 LLQRNSVD-------SHSFE--SDVGSKDGSVRNGVEGSMDVPTQDSDAKG-----NGQQ 306
             +R SVD         +FE  +  G++ G     +   +D      DA+G     +   
Sbjct: 304 KPRRTSVDGRVSVGVDRAFEKMNMWGNEIGEGGADMRKELDAELMRLDAEGLQSLKSSPV 363

Query: 307 QLRVQLKG--QGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWT 366
            +++   G  QG TISKGH+NYELMLNLQLGIRHS GR AP++S +LKPSAFDPK+K W 
Sbjct: 364 PMKLPKAGRKQGETISKGHRNYELMLNLQLGIRHSVGRQAPAASLDLKPSAFDPKDKIWR 423

Query: 367 KFPPEGSKCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGK 426
           +FP EG+K TPPHQS +F+WKDYCP+VFR+LRKLF VDPADYMLSICGNDALRELSSPGK
Sbjct: 424 RFPREGTKYTPPHQSTEFKWKDYCPLVFRSLRKLFKVDPADYMLSICGNDALRELSSPGK 483

Query: 427 SGSFFYLTHDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSS-T 486
           SGSFFYLT+DDRYMIKTM+K+E KVL+ ML AYY HV  F+N+LV +F+GLHCVKL+  T
Sbjct: 484 SGSFFYLTNDDRYMIKTMKKSETKVLLGMLAAYYNHVRAFENSLVIRFFGLHCVKLNGPT 543

Query: 487 QKKVRFVIMGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQK 530
           QKKVRFVIMGNLFCS++S+HRRFDLKGSSLGRTTDKPESEID+ T LKDLDLN+IFRLQK
Sbjct: 544 QKKVRFVIMGNLFCSKYSVHRRFDLKGSSLGRTTDKPESEIDSNTILKDLDLNFIFRLQK 603

BLAST of MS018726 vs. TAIR 10
Match: AT3G56960.1 (phosphatidyl inositol monophosphate 5 kinase 4 )

HSP 1 Score: 718.0 bits (1852), Expect = 5.6e-207
Identity = 349/550 (63.45%), Postives = 420/550 (76.36%), Query Frame = 0

Query: 4   QMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGAT 63
           ++ H E ILPNGD+Y G+W    P GHGKYLWTDGCMY+G+W +GK MG GKF WPSGAT
Sbjct: 63  ELYHAERILPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGRGKFGWPSGAT 122

Query: 64  YEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHG 123
           YEG+FKSG+MDG G +TG SGDTY+G WVMNLKHG GIK FANGD Y+GEWRRG Q   G
Sbjct: 123 YEGEFKSGYMDGVGLYTGPSGDTYKGQWVMNLKHGHGIKRFANGDVYDGEWRRGLQEAQG 182

Query: 124 RYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIW 183
           +YQWR+G++Y+G+W+N  I G GT IW +G RYDG+W+DGFP+GNGTF+W+DGSFYVG W
Sbjct: 183 KYQWRDGSYYMGEWKNATICGKGTFIWTDGNRYDGFWDDGFPRGNGTFKWADGSFYVGHW 242

Query: 184 SKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEG 243
           S +P+E +GTYYPSG  D + +WDP++VF  +L +  I   E+V + PS+K ++   S  
Sbjct: 243 SNDPEEMNGTYYPSGD-DGSPEWDPKDVF-TNLSEYKICSGERVPVLPSQKKLSVWNSSK 302

Query: 244 --ELLQRNSVD-------SHSFE------SDVGSKDGSVRNGVEGSMDVPTQDSDAKGNG 303
             E  +R SVD         +FE      ++ G     + +     +D      +A+G  
Sbjct: 303 RVEKPRRMSVDGRVSVGVDRAFEKMNMWGTESGEGAADIDSTTRRDLDAEMMRLEAEGFI 362

Query: 304 QQ------QLRVQLKG--QGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAF 363
           Q        +R+   G  QG TISKGH+NYELMLNLQLGIRH+ G+ AP  S +LK SAF
Sbjct: 363 QSLRPSPAPMRLPRAGRKQGETISKGHRNYELMLNLQLGIRHAVGKQAPVVSLDLKHSAF 422

Query: 364 DPKEKFWTKFPPEGSKCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDAL 423
           DPKEK WT+FPPEG+K TPPHQS +F+WKDYCP+VFR+LRKLF VDPADYMLSICGNDAL
Sbjct: 423 DPKEKVWTRFPPEGTKYTPPHQSSEFKWKDYCPLVFRSLRKLFKVDPADYMLSICGNDAL 482

Query: 424 RELSSPGKSGSFFYLTHDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLH 483
           RELSSPGKSGSFFYLT+DDRYMIKTM+K+E KVL+RML AYY HV  F+NTLV +FYGLH
Sbjct: 483 RELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLRMLAAYYNHVRAFENTLVIRFYGLH 542

Query: 484 CVKLSS-TQKKVRFVIMGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDL 530
           CVKL+   QKKVRFVIMGNLFCSE+SIHRRFDLKGSSLGRTTDKPESEI++ T LKDLDL
Sbjct: 543 CVKLTGPIQKKVRFVIMGNLFCSEYSIHRRFDLKGSSLGRTTDKPESEINSNTILKDLDL 602

BLAST of MS018726 vs. TAIR 10
Match: AT1G21980.1 (phosphatidylinositol-4-phosphate 5-kinase 1 )

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-168
Identity = 304/534 (56.93%), Postives = 366/534 (68.54%), Query Frame = 0

Query: 2   PAQMSHVEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSG 61
           P  +  VE  LPNGD Y G +    P G GKYLW DGCMY G+W  GK  G GKFSWPSG
Sbjct: 65  PTPLVDVEKPLPNGDLYIGSFSGGFPHGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSG 124

Query: 62  ATYEGDFKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNG 121
           ATYEG+FKSG M+G GTFTG+ GDTYRG WV + KHG G K +ANGD+YEG WRR  Q+G
Sbjct: 125 ATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDG 184

Query: 122 HGRYQWRNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVG 181
            GRY WRNGN Y G+WR+G I+G G ++W NG RY+G WE+G PKGNG F WSDGS  VG
Sbjct: 185 RGRYVWRNGNQYTGEWRSGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVG 244

Query: 182 IWSKNPKEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGS 241
            W+++    S   + +G   N+L    ++   VD                     +  GS
Sbjct: 245 AWNESNIMRS---FFNGVEKNDLIVGNRKRSSVD---------------------SGAGS 304

Query: 242 EGELLQRNSVDSHSFESDVGSKDGSVRNGVEGSM---DVPTQDSDAKGNGQQQ---LRVQ 301
            G       +     + + G     + + VE SM   D  + D D     ++       +
Sbjct: 305 LGGEKVFPRICIWESDGEAGDITCDIIDNVEASMIYRDRISVDRDGFRQFKKNPCWFNGE 364

Query: 302 LKGQGVTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGS 361
            K  G TISKGHK Y+LMLNLQLGIR+S G+ A S   +LK + FDPKEKFWT+FPPEG+
Sbjct: 365 AKKPGQTISKGHKKYDLMLNLQLGIRYSVGKHA-SIVRDLKQTDFDPKEKFWTRFPPEGT 424

Query: 362 KCTPPHQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYL 421
           K TPPHQS DFRWKDYCP+VFR LR+LF VDPA YML+ICGNDALRELSSPGKSGSFFYL
Sbjct: 425 KTTPPHQSVDFRWKDYCPLVFRRLRELFQVDPAKYMLAICGNDALRELSSPGKSGSFFYL 484

Query: 422 THDDRYMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVI 481
           T DDR+MIKT++K+EVKVL+RMLP+YYKHV  ++N+LVT+FYG+HCVK    Q K RF++
Sbjct: 485 TQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENSLVTRFYGVHCVKPVGGQ-KTRFIV 544

Query: 482 MGNLFCSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           MGNLFCSE+ I RRFDLKGSS GR+T KPE EID TTTLKDLDLN+ FRLQ+ W
Sbjct: 545 MGNLFCSEYRIQRRFDLKGSSHGRSTAKPEGEIDETTTLKDLDLNFSFRLQRNW 572

BLAST of MS018726 vs. TAIR 10
Match: AT1G77740.1 (phosphatidylinositol-4-phosphate 5-kinase 2 )

HSP 1 Score: 581.6 bits (1498), Expect = 6.2e-166
Identity = 305/529 (57.66%), Postives = 361/529 (68.24%), Query Frame = 0

Query: 8   VEMILPNGDFYAGRWVRDLPDGHGKYLWTDGCMYVGEWSDGKPMGTGKFSWPSGATYEGD 67
           VE  LPNGD Y G +    P+G GKYLW DGCMY GEW  GK  G GKFSWPSGATYEG+
Sbjct: 72  VEKPLPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGE 131

Query: 68  FKSGFMDGKGTFTGSSGDTYRGNWVMNLKHGQGIKSFANGDYYEGEWRRGSQNGHGRYQW 127
           FKSG M+G GTF G  GDTYRG+WV + K G G K +ANGDYYEG WRR  Q+G GRY W
Sbjct: 132 FKSGRMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVW 191

Query: 128 RNGNHYIGKWRNGKINGNGTMIWRNGKRYDGYWEDGFPKGNGTFRWSDGSFYVGIWSKNP 187
            NGN Y G+WRNG I G G + W NG RY+G WE+G PKG+G F W+DGS ++G W+++ 
Sbjct: 192 MNGNQYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSWNES- 251

Query: 188 KEHSGTYYPSGSTDNNLDWDPQEVFDVDLVDCLIYPCEKVSIFPSEKLVNKGGSEGELLQ 247
                         +NL    +  FD    + LI    K S   S      G   GE + 
Sbjct: 252 --------------SNL---MRNFFDGIEKNELIVATRKRSSVDS----GAGSLTGEKIF 311

Query: 248 RNSVDSHSFESDVGSKDGSVRNGVEGSMDVPTQDSDAKGNGQQQLRV-------QLKGQG 307
                  S + + G     + + VE S+    + S  K +G +Q R        + K  G
Sbjct: 312 PRICIWES-DGEAGDITCDIVDNVEASVIYRDRISIDK-DGFRQFRKNPCCFSGEAKKPG 371

Query: 308 VTISKGHKNYELMLNLQLGIRHSAGRPAPSSSFELKPSAFDPKEKFWTKFPPEGSKCTPP 367
            TISKGHK Y+LMLNLQ GIR+S G+ A S   +LK S FDP EKFWT+FPPEGSK TPP
Sbjct: 372 ETISKGHKKYDLMLNLQHGIRYSVGKHA-SVVRDLKQSDFDPSEKFWTRFPPEGSKTTPP 431

Query: 368 HQSCDFRWKDYCPVVFRTLRKLFNVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDR 427
           H S DFRWKDYCP+VFR LR+LF VDPADYML+ICGNDALRELSSPGKSGSFFYLT DDR
Sbjct: 432 HLSVDFRWKDYCPLVFRRLRELFTVDPADYMLAICGNDALRELSSPGKSGSFFYLTQDDR 491

Query: 428 YMIKTMRKAEVKVLIRMLPAYYKHVEDFKNTLVTKFYGLHCVKLSSTQKKVRFVIMGNLF 487
           +MIKT++K+EVKVL+RMLP+YYKHV  ++NTLVT+FYG+HC+K    Q K RF++MGNLF
Sbjct: 492 FMIKTVKKSEVKVLLRMLPSYYKHVCQYENTLVTRFYGVHCIKPVGGQ-KTRFIVMGNLF 551

Query: 488 CSEHSIHRRFDLKGSSLGRTTDKPESEIDATTTLKDLDLNYIFRLQKIW 530
           CSE+ I RRFDLKGSS GR T KPE EID TTTLKDLDLN+ FRLQ+ W
Sbjct: 552 CSEYRIQRRFDLKGSSHGRYTSKPEGEIDETTTLKDLDLNFAFRLQRNW 574

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146326.10.0e+00100.00phosphatidylinositol 4-phosphate 5-kinase 6-like [Momordica charantia][more]
XP_038875534.11.4e-29791.48phosphatidylinositol 4-phosphate 5-kinase 6-like isoform X1 [Benincasa hispida][more]
XP_011655253.11.9e-29490.17phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis sativus] >XP_011655254.1 ph... [more]
XP_008465842.13.3e-29490.74PREDICTED: phosphatidylinositol 4-phosphate 5-kinase 6-like [Cucumis melo] >XP_0... [more]
XP_022993786.11.1e-27887.10phosphatidylinositol 4-phosphate 5-kinase 6-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SFB85.4e-22370.06Phosphatidylinositol 4-phosphate 5-kinase 6 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9SLG91.6e-20663.65Phosphatidylinositol 4-phosphate 5-kinase 5 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9M1K27.8e-20663.45Phosphatidylinositol 4-phosphate 5-kinase 4 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q56YP21.9e-16756.93Phosphatidylinositol 4-phosphate 5-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q8L7968.8e-16557.66Phosphatidylinositol 4-phosphate 5-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1CZ150.0e+00100.00Phosphatidylinositol 4-phosphate 5-kinase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0KRS09.4e-29590.17Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G43... [more]
A0A1S3CR891.6e-29490.74Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo OX=3656 GN=LOC10350343... [more]
A0A5D3BGP01.6e-29490.74Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1JZH25.5e-27987.10Phosphatidylinositol 4-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G07960.13.8e-22470.06Phosphatidylinositol-4-phosphate 5-kinase family protein [more]
AT2G41210.11.1e-20763.65phosphatidylinositol- 4-phosphate 5-kinase 5 [more]
AT3G56960.15.6e-20763.45phosphatidyl inositol monophosphate 5 kinase 4 [more]
AT1G21980.11.3e-16856.93phosphatidylinositol-4-phosphate 5-kinase 1 [more]
AT1G77740.16.2e-16657.66phosphatidylinositol-4-phosphate 5-kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003409MORN motifSMARTSM00698morncoord: 131..152
e-value: 1.5E-4
score: 31.2
coord: 108..129
e-value: 0.0048
score: 26.1
coord: 62..83
e-value: 0.028
score: 23.5
coord: 39..60
e-value: 0.0087
score: 25.3
coord: 16..37
e-value: 1.9E-4
score: 30.8
coord: 154..175
e-value: 0.076
score: 22.1
coord: 85..106
e-value: 4.3E-6
score: 36.2
IPR003409MORN motifPFAMPF02493MORNcoord: 133..155
e-value: 5.8E-6
score: 25.9
coord: 156..177
e-value: 2.3E-4
score: 20.8
coord: 41..62
e-value: 2.0E-6
score: 27.3
coord: 18..40
e-value: 1.8E-6
score: 27.5
coord: 110..131
e-value: 1.7E-5
score: 24.4
coord: 87..108
e-value: 2.1E-5
score: 24.1
coord: 64..86
e-value: 2.6E-5
score: 23.9
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, coreSMARTSM00330PIPK_2coord: 336..529
e-value: 1.9E-62
score: 223.4
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePFAMPF01504PIP5Kcoord: 397..526
e-value: 4.8E-52
score: 176.9
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePROSITEPS51455PIPKcoord: 302..529
score: 45.181301
NoneNo IPR availableGENE3D2.20.110.10coord: 6..47
e-value: 2.9E-7
score: 32.1
coord: 93..139
e-value: 2.4E-12
score: 48.4
coord: 140..197
e-value: 1.8E-9
score: 39.2
NoneNo IPR availableGENE3D3.30.810.10coord: 479..528
e-value: 3.1E-8
score: 35.5
NoneNo IPR availableGENE3D2.20.110.10coord: 48..92
e-value: 2.5E-5
score: 25.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..288
NoneNo IPR availablePANTHERPTHR23086:SF29PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE 4coord: 4..529
NoneNo IPR availableCDDcd17302PIPKc_AtPIP5K_likecoord: 309..529
e-value: 8.67767E-154
score: 439.803
NoneNo IPR availableSUPERFAMILY82185Histone H3 K4-specific methyltransferase SET7/9 N-terminal domaincoord: 12..117
NoneNo IPR availableSUPERFAMILY82185Histone H3 K4-specific methyltransferase SET7/9 N-terminal domaincoord: 104..199
NoneNo IPR availableSUPERFAMILY56104SAICAR synthase-likecoord: 305..527
IPR027484Phosphatidylinositol-4-phosphate 5-kinase, N-terminalGENE3D3.30.800.10Phosphatidylinositol Phosphate Kinase II Betacoord: 296..478
e-value: 8.9E-67
score: 226.5
IPR023610Phosphatidylinositol-4-phosphate 5-kinasePANTHERPTHR23086PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASEcoord: 4..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018726.1MS018726.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046488 phosphatidylinositol metabolic process
molecular_function GO:0016307 phosphatidylinositol phosphate kinase activity