MS018690 (gene) Bitter gourd (TR) v1

Overview
NameMS018690
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionethylene-responsive transcription factor LEP
Locationscaffold313: 1086990 .. 1087658 (-)
RNA-Seq ExpressionMS018690
SyntenyMS018690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCCATCGCCGTCGAAGCCTAAGCGGAAGCAGCCGCCGCCGACGCCGGAAACGGCGTCGCACCGTTTCCTTGGCGTTCGGCGGCGGCCGTGGGGGCGATACGCCGCCGAGATTAGAGATCCTTCCACCAAAGAACGCCACTGGCTCGGCACCTTCGACACCGCCGAGGAAGCCGCCCTCGCCTACGACCGCGCCGCCCGCTCCCTCCGCGGCTCCCTCGCCCGCACCAACTTCCTTTACTCCGACTCGCCGCCGCCGCTGCCGCCCTCCGCCGCCGTCTCTCACGGCCAGCTTCCGCCACCTCCCCTGCCTCCTCCTCTGTTTCTTCCTCTGCTTCCTCCAAATCCGCCGCCTCTCTTCGACCAATTCCCCGCCGCTGGAGACTCCTCGGCAAGTTTCTTCGCTGGATTCGACGATGGCGGTGCTGAGCTTCCGCCATTTCCTCCGGCAATTTCGTCGGAATCGGAGAATTATAATTATAATAACTGTCTGATGGAAACACAGAACATTGGTTTAGAATCTCTTGATCAGTCGTCGATGGGAATCGGATCGTACTTCGGATTTGATTCCGGCGGTGAGTACGTGCAGAGCCCGATCTTTGGAACAATGCCTGCGGTTTCCGACGCCCTCGCCAGTGATTTTGAGTCTCCGGCCAATTTCTATTTC

mRNA sequence

ATGAGTCCATCGCCGTCGAAGCCTAAGCGGAAGCAGCCGCCGCCGACGCCGGAAACGGCGTCGCACCGTTTCCTTGGCGTTCGGCGGCGGCCGTGGGGGCGATACGCCGCCGAGATTAGAGATCCTTCCACCAAAGAACGCCACTGGCTCGGCACCTTCGACACCGCCGAGGAAGCCGCCCTCGCCTACGACCGCGCCGCCCGCTCCCTCCGCGGCTCCCTCGCCCGCACCAACTTCCTTTACTCCGACTCGCCGCCGCCGCTGCCGCCCTCCGCCGCCGTCTCTCACGGCCAGCTTCCGCCACCTCCCCTGCCTCCTCCTCTGTTTCTTCCTCTGCTTCCTCCAAATCCGCCGCCTCTCTTCGACCAATTCCCCGCCGCTGGAGACTCCTCGGCAAGTTTCTTCGCTGGATTCGACGATGGCGGTGCTGAGCTTCCGCCATTTCCTCCGGCAATTTCGTCGGAATCGGAGAATTATAATTATAATAACTGTCTGATGGAAACACAGAACATTGGTTTAGAATCTCTTGATCAGTCGTCGATGGGAATCGGATCGTACTTCGGATTTGATTCCGGCGGTGAGTACGTGCAGAGCCCGATCTTTGGAACAATGCCTGCGGTTTCCGACGCCCTCGCCAGTGATTTTGAGTCTCCGGCCAATTTCTATTTC

Coding sequence (CDS)

ATGAGTCCATCGCCGTCGAAGCCTAAGCGGAAGCAGCCGCCGCCGACGCCGGAAACGGCGTCGCACCGTTTCCTTGGCGTTCGGCGGCGGCCGTGGGGGCGATACGCCGCCGAGATTAGAGATCCTTCCACCAAAGAACGCCACTGGCTCGGCACCTTCGACACCGCCGAGGAAGCCGCCCTCGCCTACGACCGCGCCGCCCGCTCCCTCCGCGGCTCCCTCGCCCGCACCAACTTCCTTTACTCCGACTCGCCGCCGCCGCTGCCGCCCTCCGCCGCCGTCTCTCACGGCCAGCTTCCGCCACCTCCCCTGCCTCCTCCTCTGTTTCTTCCTCTGCTTCCTCCAAATCCGCCGCCTCTCTTCGACCAATTCCCCGCCGCTGGAGACTCCTCGGCAAGTTTCTTCGCTGGATTCGACGATGGCGGTGCTGAGCTTCCGCCATTTCCTCCGGCAATTTCGTCGGAATCGGAGAATTATAATTATAATAACTGTCTGATGGAAACACAGAACATTGGTTTAGAATCTCTTGATCAGTCGTCGATGGGAATCGGATCGTACTTCGGATTTGATTCCGGCGGTGAGTACGTGCAGAGCCCGATCTTTGGAACAATGCCTGCGGTTTCCGACGCCCTCGCCAGTGATTTTGAGTCTCCGGCCAATTTCTATTTC

Protein sequence

MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPLFDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF
Homology
BLAST of MS018690 vs. NCBI nr
Match: XP_022141682.1 (ethylene-responsive transcription factor LEP [Momordica charantia])

HSP 1 Score: 437.2 bits (1123), Expect = 8.6e-119
Identity = 220/223 (98.65%), Postives = 221/223 (99.10%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQPPP PETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQPPPPPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPP+ PPL
Sbjct: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPDSPPL 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSS 180
           FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSS
Sbjct: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSS 180

Query: 181 MGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF 224
           MGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF
Sbjct: 181 MGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF 223

BLAST of MS018690 vs. NCBI nr
Match: KAA0066104.1 (ethylene-responsive transcription factor LEP [Cucumis melo var. makuwa] >TYK15143.1 ethylene-responsive transcription factor LEP [Cucumis melo var. makuwa])

HSP 1 Score: 288.5 bits (737), Expect = 4.9e-74
Identity = 160/226 (70.80%), Postives = 175/226 (77.43%), Query Frame = 0

Query: 1   MSPSPSKPKRK-QPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEA 60
           MSPSPSKPKRK  PPP  E   HRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEA
Sbjct: 1   MSPSPSKPKRKHHPPPPAEPTPHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEA 60

Query: 61  ALAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPP 120
           A+AYDRAARSLRGSLARTNFLYSDSPPP PP  + S       P  PPLFLPLLPP PPP
Sbjct: 61  AIAYDRAARSLRGSLARTNFLYSDSPPPPPPPYSAS-----IHPSQPPLFLPLLPPPPPP 120

Query: 121 LFDQFPAAGDS-SASFFAGFDDGGAELPPFPPAISSESENYNYNNC-LMETQNIGLESLD 180
           LF QFP AGDS S   +  +D   AELPPFPPAISSESEN +YNN   ME+QN+G E  D
Sbjct: 121 LFHQFPPAGDSPSGIIYGDYDGSSAELPPFPPAISSESENCDYNNYGAMESQNVGFECFD 180

Query: 181 QSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFY 223
           QSS+G+GS  GFDS G+Y+ +P+ G+M  VSDA   D +SP ANFY
Sbjct: 181 QSSIGMGSCLGFDSSGDYMYNPMLGSMATVSDAGTGDLQSPAANFY 221

BLAST of MS018690 vs. NCBI nr
Match: XP_022990620.1 (ethylene-responsive transcription factor LEP-like [Cucurbita maxima])

HSP 1 Score: 286.2 bits (731), Expect = 2.4e-73
Identity = 165/228 (72.37%), Postives = 169/228 (74.12%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQ  P PET SHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQQQPPPETTSHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           +AYD AARSLRGSLARTNFLYSDS PP PP         PP    PP   PLL P PPP 
Sbjct: 61  VAYDCAARSLRGSLARTNFLYSDSLPPPPP---------PPSAALPPNQAPLLLPTPPPH 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESE----NYNYNNCLMETQNIGLESL 180
           F Q P + DS  S F+   DGGAELPPFPPAISSESE    NYNYN  LME QN G E  
Sbjct: 121 FHQIPPSRDSPPSLFSVDGDGGAELPPFPPAISSESENYNYNYNYNQGLMELQNSGFEYF 180

Query: 181 DQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFYF 224
           DQSS  IGS FGFDS  EYV  PIF +MPAVSDA A DF SP ANFYF
Sbjct: 181 DQSSTEIGSCFGFDSSSEYVHGPIFSSMPAVSDAGAGDFPSPAANFYF 219

BLAST of MS018690 vs. NCBI nr
Match: XP_023541749.1 (ethylene-responsive transcription factor LEP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282.7 bits (722), Expect = 2.7e-72
Identity = 163/228 (71.49%), Postives = 168/228 (73.68%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQ  P PET  HRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQQQPPPETTPHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           +AYD AARSLRGSLARTNFLYSDS PP PP         PP    PP   PLL P PPP 
Sbjct: 61  VAYDCAARSLRGSLARTNFLYSDSLPPPPP---------PPSAALPPNQAPLLLPTPPPH 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESE----NYNYNNCLMETQNIGLESL 180
           F Q P + DS  S F+   DGGAELPPFPPAISSESE    NYNYN  LME QN G E  
Sbjct: 121 FHQIPPSRDSPPSLFSVDGDGGAELPPFPPAISSESENYNYNYNYNQGLMELQNSGFEYF 180

Query: 181 DQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFYF 224
           DQS   IGS FGFDS  EYV  PIFG+MPAVSDA A +F SP ANFYF
Sbjct: 181 DQSPTDIGSCFGFDSSSEYVHGPIFGSMPAVSDAGAGEFLSPAANFYF 219

BLAST of MS018690 vs. NCBI nr
Match: XP_022963669.1 (ethylene-responsive transcription factor LEP-like [Cucurbita moschata] >KAG6601967.1 Ethylene-responsive transcription factor LEP, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 277.3 bits (708), Expect = 1.1e-70
Identity = 161/232 (69.40%), Postives = 167/232 (71.98%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQ  P PET  HRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQQQPPPETTPHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           +AYD AARSLRGSLARTNFLYSDS PP PP         PP    PP   PLL P PPP 
Sbjct: 61  VAYDCAARSLRGSLARTNFLYSDSLPPPPP---------PPSAALPPNQAPLLLPTPPPH 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESE--------NYNYNNCLMETQNIG 180
           F Q P + DS  S F+   DGGAELPPFPPAISSESE        NYNYN  LME QN G
Sbjct: 121 FHQIPPSRDSPPSLFSVDGDGGAELPPFPPAISSESENYNYNYNYNYNYNQGLMELQNSG 180

Query: 181 LESLDQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFYF 224
            E  +QS   IGS FGFDS  EYV  PIF +MPAVSDA A +F SP ANFYF
Sbjct: 181 FEYFNQSPTEIGSCFGFDSSSEYVHGPIFSSMPAVSDAGAGEFPSPAANFYF 223

BLAST of MS018690 vs. ExPASy Swiss-Prot
Match: Q9M644 (Ethylene-responsive transcription factor LEP OS=Arabidopsis thaliana OX=3702 GN=LEP PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.6e-35
Identity = 103/233 (44.21%), Postives = 129/233 (55.36%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           M+ + SK K+KQ     +    RFLGVRRRPWGRYAAEIRDP+TKERHWLGTFDTAEEAA
Sbjct: 1   MNTTSSKSKKKQ----DDQVGTRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPP-----------LF 120
           LAYDRAARS+RG+ ARTNF+YSD PP    ++ VS    PPPP PP            +F
Sbjct: 61  LAYDRAARSMRGTRARTNFVYSDMPPSSSVTSIVSPDDPPPPPPPPAPPSNDPVDYMMMF 120

Query: 121 -------LPLLPPNPPPLFDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYN 180
                   P+L P+   + D +   G  S++ +   +D   ELPP P  +S        N
Sbjct: 121 NQYSSTDSPMLQPHCDQV-DSYMFGGSQSSNSYCYSNDSSNELPPLPSDLS--------N 180

Query: 181 NCLMETQNIGLESLDQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDF 216
           +C  + Q               ++ G D   EYV SP+F  MP VSD+    F
Sbjct: 181 SCYSQPQ--------------WTWTGDDYSSEYVHSPMFSRMPPVSDSFPQGF 206

BLAST of MS018690 vs. ExPASy Swiss-Prot
Match: Q9FZ90 (Ethylene-responsive transcription factor ERF087 OS=Arabidopsis thaliana OX=3702 GN=ERF087 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.4e-25
Identity = 59/88 (67.05%), Postives = 70/88 (79.55%), Query Frame = 0

Query: 2   SPSPSKPKRKQPPPTPETASH----RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAE 61
           S S    ++KQ  P P+   H    +++GVRRRPWGRYAAEIR+P+TKER+WLGTFDTAE
Sbjct: 13  SRSKKSHRQKQQQPQPQPQQHIEEIKYVGVRRRPWGRYAAEIRNPTTKERYWLGTFDTAE 72

Query: 62  EAALAYDRAARSLRGSLARTNFLYSDSP 86
           EAALAYDRAARS+RG  ARTNF+YSD P
Sbjct: 73  EAALAYDRAARSIRGLTARTNFVYSDMP 100

BLAST of MS018690 vs. ExPASy Swiss-Prot
Match: Q6J9Q2 (Ethylene-responsive transcription factor ERF086 OS=Arabidopsis thaliana OX=3702 GN=ERF086 PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 2.8e-24
Identity = 52/60 (86.67%), Postives = 57/60 (95.00%), Query Frame = 0

Query: 23  RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYDRAARSLRGSLARTNFLYS 82
           RFLGVRRRPWGRYAAEIRDP+TKERHWLGTFDTA EAALAYDRAA S+RG+ ARTNF+Y+
Sbjct: 54  RFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAHEAALAYDRAALSMRGTQARTNFVYT 113

BLAST of MS018690 vs. ExPASy Swiss-Prot
Match: Q8H3Q1 (Ethylene-responsive transcription factor FZP OS=Oryza sativa subsp. japonica OX=39947 GN=FZP PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.9e-24
Identity = 60/99 (60.61%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 3   PSPSKPKRKQPPPTPETASH-----RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAE 62
           PSP      + PP+  +        RFLGVRRRPWGRYAAEIRDP+TKERHWLGTFDTA+
Sbjct: 32  PSPPSSPMSERPPSGRSRRRAQEPGRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAQ 91

Query: 63  EAALAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSH 97
           EAALAYDRAA S++G+ ARTNF+Y+ +    PP  A  H
Sbjct: 92  EAALAYDRAALSMKGAQARTNFVYTHAAYNYPPFLAPFH 130

BLAST of MS018690 vs. ExPASy Swiss-Prot
Match: Q3E703 (Ethylene-responsive transcription factor ERF088 OS=Arabidopsis thaliana OX=3702 GN=ERF088 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.9e-21
Identity = 55/84 (65.48%), Postives = 64/84 (76.19%), Query Frame = 0

Query: 1  MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
          M  S +K K K+     E    ++LGVRRRPWGRYAAEIR+P TKERHWLGTFDTAEEAA
Sbjct: 1  MLKSSNKRKSKEEKKLQE---GKYLGVRRRPWGRYAAEIRNPFTKERHWLGTFDTAEEAA 60

Query: 61 LAYDRAARSLRGSLARTNFLYSDS 85
           AYD AARS+ GSLA TNF Y+++
Sbjct: 61 FAYDVAARSISGSLATTNFFYTEN 81

BLAST of MS018690 vs. ExPASy TrEMBL
Match: A0A6J1CKI8 (ethylene-responsive transcription factor LEP OS=Momordica charantia OX=3673 GN=LOC111011985 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.2e-119
Identity = 220/223 (98.65%), Postives = 221/223 (99.10%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQPPP PETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQPPPPPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPP+ PPL
Sbjct: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPDSPPL 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSS 180
           FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSS
Sbjct: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYNNCLMETQNIGLESLDQSS 180

Query: 181 MGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF 224
           MGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF
Sbjct: 181 MGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESPANFYF 223

BLAST of MS018690 vs. ExPASy TrEMBL
Match: A0A5A7VFL7 (Ethylene-responsive transcription factor LEP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00060 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.4e-74
Identity = 160/226 (70.80%), Postives = 175/226 (77.43%), Query Frame = 0

Query: 1   MSPSPSKPKRK-QPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEA 60
           MSPSPSKPKRK  PPP  E   HRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEA
Sbjct: 1   MSPSPSKPKRKHHPPPPAEPTPHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEA 60

Query: 61  ALAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPP 120
           A+AYDRAARSLRGSLARTNFLYSDSPPP PP  + S       P  PPLFLPLLPP PPP
Sbjct: 61  AIAYDRAARSLRGSLARTNFLYSDSPPPPPPPYSAS-----IHPSQPPLFLPLLPPPPPP 120

Query: 121 LFDQFPAAGDS-SASFFAGFDDGGAELPPFPPAISSESENYNYNNC-LMETQNIGLESLD 180
           LF QFP AGDS S   +  +D   AELPPFPPAISSESEN +YNN   ME+QN+G E  D
Sbjct: 121 LFHQFPPAGDSPSGIIYGDYDGSSAELPPFPPAISSESENCDYNNYGAMESQNVGFECFD 180

Query: 181 QSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFY 223
           QSS+G+GS  GFDS G+Y+ +P+ G+M  VSDA   D +SP ANFY
Sbjct: 181 QSSIGMGSCLGFDSSGDYMYNPMLGSMATVSDAGTGDLQSPAANFY 221

BLAST of MS018690 vs. ExPASy TrEMBL
Match: A0A6J1JQK9 (ethylene-responsive transcription factor LEP-like OS=Cucurbita maxima OX=3661 GN=LOC111487448 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.2e-73
Identity = 165/228 (72.37%), Postives = 169/228 (74.12%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQ  P PET SHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQQQPPPETTSHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           +AYD AARSLRGSLARTNFLYSDS PP PP         PP    PP   PLL P PPP 
Sbjct: 61  VAYDCAARSLRGSLARTNFLYSDSLPPPPP---------PPSAALPPNQAPLLLPTPPPH 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESE----NYNYNNCLMETQNIGLESL 180
           F Q P + DS  S F+   DGGAELPPFPPAISSESE    NYNYN  LME QN G E  
Sbjct: 121 FHQIPPSRDSPPSLFSVDGDGGAELPPFPPAISSESENYNYNYNYNQGLMELQNSGFEYF 180

Query: 181 DQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFYF 224
           DQSS  IGS FGFDS  EYV  PIF +MPAVSDA A DF SP ANFYF
Sbjct: 181 DQSSTEIGSCFGFDSSSEYVHGPIFSSMPAVSDAGAGDFPSPAANFYF 219

BLAST of MS018690 vs. ExPASy TrEMBL
Match: A0A6J1HKV8 (ethylene-responsive transcription factor LEP-like OS=Cucurbita moschata OX=3662 GN=LOC111463929 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 5.5e-71
Identity = 161/232 (69.40%), Postives = 167/232 (71.98%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           MSPSPSKPKRKQ  P PET  HRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA
Sbjct: 1   MSPSPSKPKRKQQQPPPETTPHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPL 120
           +AYD AARSLRGSLARTNFLYSDS PP PP         PP    PP   PLL P PPP 
Sbjct: 61  VAYDCAARSLRGSLARTNFLYSDSLPPPPP---------PPSAALPPNQAPLLLPTPPPH 120

Query: 121 FDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESE--------NYNYNNCLMETQNIG 180
           F Q P + DS  S F+   DGGAELPPFPPAISSESE        NYNYN  LME QN G
Sbjct: 121 FHQIPPSRDSPPSLFSVDGDGGAELPPFPPAISSESENYNYNYNYNYNYNQGLMELQNSG 180

Query: 181 LESLDQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFYF 224
            E  +QS   IGS FGFDS  EYV  PIF +MPAVSDA A +F SP ANFYF
Sbjct: 181 FEYFNQSPTEIGSCFGFDSSSEYVHGPIFSSMPAVSDAGAGEFPSPAANFYF 223

BLAST of MS018690 vs. ExPASy TrEMBL
Match: A0A1S3AU89 (ethylene-responsive transcription factor LEP OS=Cucumis melo OX=3656 GN=LOC103483013 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.8e-69
Identity = 150/221 (67.87%), Postives = 165/221 (74.66%), Query Frame = 0

Query: 5   PSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYD 64
           P  PK    P   E   HRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA+AYD
Sbjct: 5   PQNPKESTTPAPAEPTPHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAAIAYD 64

Query: 65  RAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPPLFLPLLPPNPPPLFDQF 124
           RAARSLRGSLARTNFLYSDSPPP PP  + S       P  PPLFLPLLPP PPPLF QF
Sbjct: 65  RAARSLRGSLARTNFLYSDSPPPPPPPYSAS-----IHPSQPPLFLPLLPPPPPPLFHQF 124

Query: 125 PAAGDS-SASFFAGFDDGGAELPPFPPAISSESENYNYNNC-LMETQNIGLESLDQSSMG 184
           P AGDS S   +  +D   AELPPFPPAISSESEN +YNN   ME+QN+G E  DQSS+G
Sbjct: 125 PPAGDSPSGIIYGDYDGSSAELPPFPPAISSESENCDYNNYGAMESQNVGFECFDQSSIG 184

Query: 185 IGSYFGFDSGGEYVQSPIFGTMPAVSDALASDFESP-ANFY 223
           +GS  GFDS G+Y+ +P+ G+M  VSDA   D +SP ANFY
Sbjct: 185 MGSCLGFDSSGDYMYNPMLGSMATVSDAGTGDLQSPAANFY 220

BLAST of MS018690 vs. TAIR 10
Match: AT5G13910.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 149.8 bits (377), Expect = 2.5e-36
Identity = 103/233 (44.21%), Postives = 129/233 (55.36%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
           M+ + SK K+KQ     +    RFLGVRRRPWGRYAAEIRDP+TKERHWLGTFDTAEEAA
Sbjct: 1   MNTTSSKSKKKQ----DDQVGTRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAEEAA 60

Query: 61  LAYDRAARSLRGSLARTNFLYSDSPPPLPPSAAVSHGQLPPPPLPPP-----------LF 120
           LAYDRAARS+RG+ ARTNF+YSD PP    ++ VS    PPPP PP            +F
Sbjct: 61  LAYDRAARSMRGTRARTNFVYSDMPPSSSVTSIVSPDDPPPPPPPPAPPSNDPVDYMMMF 120

Query: 121 -------LPLLPPNPPPLFDQFPAAGDSSASFFAGFDDGGAELPPFPPAISSESENYNYN 180
                   P+L P+   + D +   G  S++ +   +D   ELPP P  +S        N
Sbjct: 121 NQYSSTDSPMLQPHCDQV-DSYMFGGSQSSNSYCYSNDSSNELPPLPSDLS--------N 180

Query: 181 NCLMETQNIGLESLDQSSMGIGSYFGFDSGGEYVQSPIFGTMPAVSDALASDF 216
           +C  + Q               ++ G D   EYV SP+F  MP VSD+    F
Sbjct: 181 SCYSQPQ--------------WTWTGDDYSSEYVHSPMFSRMPPVSDSFPQGF 206

BLAST of MS018690 vs. TAIR 10
Match: AT1G28160.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 3.1e-26
Identity = 59/88 (67.05%), Postives = 70/88 (79.55%), Query Frame = 0

Query: 2   SPSPSKPKRKQPPPTPETASH----RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAE 61
           S S    ++KQ  P P+   H    +++GVRRRPWGRYAAEIR+P+TKER+WLGTFDTAE
Sbjct: 13  SRSKKSHRQKQQQPQPQPQQHIEEIKYVGVRRRPWGRYAAEIRNPTTKERYWLGTFDTAE 72

Query: 62  EAALAYDRAARSLRGSLARTNFLYSDSP 86
           EAALAYDRAARS+RG  ARTNF+YSD P
Sbjct: 73  EAALAYDRAARSIRGLTARTNFVYSDMP 100

BLAST of MS018690 vs. TAIR 10
Match: AT5G18560.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-25
Identity = 52/60 (86.67%), Postives = 57/60 (95.00%), Query Frame = 0

Query: 23  RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYDRAARSLRGSLARTNFLYS 82
           RFLGVRRRPWGRYAAEIRDP+TKERHWLGTFDTA EAALAYDRAA S+RG+ ARTNF+Y+
Sbjct: 54  RFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAHEAALAYDRAALSMRGTQARTNFVYT 113

BLAST of MS018690 vs. TAIR 10
Match: AT1G12890.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.7e-22
Identity = 55/84 (65.48%), Postives = 64/84 (76.19%), Query Frame = 0

Query: 1  MSPSPSKPKRKQPPPTPETASHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAA 60
          M  S +K K K+     E    ++LGVRRRPWGRYAAEIR+P TKERHWLGTFDTAEEAA
Sbjct: 1  MLKSSNKRKSKEEKKLQE---GKYLGVRRRPWGRYAAEIRNPFTKERHWLGTFDTAEEAA 60

Query: 61 LAYDRAARSLRGSLARTNFLYSDS 85
           AYD AARS+ GSLA TNF Y+++
Sbjct: 61 FAYDVAARSISGSLATTNFFYTEN 81

BLAST of MS018690 vs. TAIR 10
Match: AT1G24590.1 (DORNROSCHEN-like )

HSP 1 Score: 99.8 bits (247), Expect = 3.0e-21
Identity = 52/82 (63.41%), Postives = 60/82 (73.17%), Query Frame = 0

Query: 23  RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYDRAARSLRGSLARTNFLYS 82
           R+ GVRRRPWGRYAAEIRDP +KER WLGTFDTAEEAA AYD AAR++RG  ARTNF+Y 
Sbjct: 57  RYRGVRRRPWGRYAAEIRDPLSKERRWLGTFDTAEEAACAYDCAARAMRGLKARTNFVY- 116

Query: 83  DSPPPLPPSAAVSHGQLPPPPL 105
               P+P   +  H     PP+
Sbjct: 117 ----PMPSLDSYHHRIFSSPPM 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141682.18.6e-11998.65ethylene-responsive transcription factor LEP [Momordica charantia][more]
KAA0066104.14.9e-7470.80ethylene-responsive transcription factor LEP [Cucumis melo var. makuwa] >TYK1514... [more]
XP_022990620.12.4e-7372.37ethylene-responsive transcription factor LEP-like [Cucurbita maxima][more]
XP_023541749.12.7e-7271.49ethylene-responsive transcription factor LEP-like [Cucurbita pepo subsp. pepo][more]
XP_022963669.11.1e-7069.40ethylene-responsive transcription factor LEP-like [Cucurbita moschata] >KAG66019... [more]
Match NameE-valueIdentityDescription
Q9M6443.6e-3544.21Ethylene-responsive transcription factor LEP OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FZ904.4e-2567.05Ethylene-responsive transcription factor ERF087 OS=Arabidopsis thaliana OX=3702 ... [more]
Q6J9Q22.8e-2486.67Ethylene-responsive transcription factor ERF086 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8H3Q14.9e-2460.61Ethylene-responsive transcription factor FZP OS=Oryza sativa subsp. japonica OX=... [more]
Q3E7033.9e-2165.48Ethylene-responsive transcription factor ERF088 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CKI84.2e-11998.65ethylene-responsive transcription factor LEP OS=Momordica charantia OX=3673 GN=L... [more]
A0A5A7VFL72.4e-7470.80Ethylene-responsive transcription factor LEP OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1JQK91.2e-7372.37ethylene-responsive transcription factor LEP-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1HKV85.5e-7169.40ethylene-responsive transcription factor LEP-like OS=Cucurbita moschata OX=3662 ... [more]
A0A1S3AU891.8e-6967.87ethylene-responsive transcription factor LEP OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT5G13910.12.5e-3644.21Integrase-type DNA-binding superfamily protein [more]
AT1G28160.13.1e-2667.05Integrase-type DNA-binding superfamily protein [more]
AT5G18560.12.0e-2586.67Integrase-type DNA-binding superfamily protein [more]
AT1G12890.12.7e-2265.48Integrase-type DNA-binding superfamily protein [more]
AT1G24590.13.0e-2163.41DORNROSCHEN-like [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 46..62
score: 69.2
coord: 24..35
score: 53.33
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 23..86
e-value: 3.1E-34
score: 129.7
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 26..72
e-value: 4.1E-13
score: 49.4
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 23..80
score: 20.573601
IPR001471AP2/ERF domainCDDcd00018AP2coord: 23..79
e-value: 4.58676E-30
score: 104.276
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 22..81
e-value: 9.1E-25
score: 88.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR31677AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 10..87
NoneNo IPR availablePANTHERPTHR31677:SF118ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR LEPcoord: 140..216
NoneNo IPR availablePANTHERPTHR31677:SF118ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR LEPcoord: 10..87
NoneNo IPR availablePANTHERPTHR31677AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 140..216
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 24..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018690.1MS018690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity