MS018688 (gene) Bitter gourd (TR) v1

Overview
NameMS018688
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionHeavy metal-associated domain containing protein
Locationscaffold313: 1068583 .. 1068744 (-)
RNA-Seq ExpressionMS018688
SyntenyMS018688
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTTTCAGGTGTTTTGAGAATAGAAATTGACCCACTACAGCCAAAAGTGAGCATTTTTGGTAATGTTGACCCCCAAATTCTCATAAAGAAGCTTCATAAAGCTGGAAAACAAGCACAAATTTGGAGCCAAGGCAATAACAATTTCAATCAAATTAATTAC

mRNA sequence

GAATTTTCAGGTGTTTTGAGAATAGAAATTGACCCACTACAGCCAAAAGTGAGCATTTTTGGTAATGTTGACCCCCAAATTCTCATAAAGAAGCTTCATAAAGCTGGAAAACAAGCACAAATTTGGAGCCAAGGCAATAACAATTTCAATCAAATTAATTAC

Coding sequence (CDS)

GAATTTTCAGGTGTTTTGAGAATAGAAATTGACCCACTACAGCCAAAAGTGAGCATTTTTGGTAATGTTGACCCCCAAATTCTCATAAAGAAGCTTCATAAAGCTGGAAAACAAGCACAAATTTGGAGCCAAGGCAATAACAATTTCAATCAAATTAATTAC

Protein sequence

EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNNFNQINY
Homology
BLAST of MS018688 vs. NCBI nr
Match: XP_034708189.1 (heavy metal-associated isoprenylated plant protein 32-like [Vitis riparia])

HSP 1 Score: 77.0 bits (188), Expect = 5.5e-11
Identity = 35/50 (70.00%), Postives = 41/50 (82.00%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNNFNQIN 54
          GVL+ EIDPLQPKV++ GNVDP+ILIKKL K GKQA+IWS GN N  + N
Sbjct: 35 GVLKTEIDPLQPKVTVVGNVDPKILIKKLQKCGKQAEIWSSGNQNAGKQN 84

BLAST of MS018688 vs. NCBI nr
Match: RVW45064.1 (Heavy metal-associated isoprenylated plant protein 32 [Vitis vinifera])

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-10
Identity = 34/50 (68.00%), Postives = 41/50 (82.00%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNNFNQIN 54
          GVL+ EIDPLQPKV++ GNVDP+ILIKKL + GKQA+IWS GN N  + N
Sbjct: 35 GVLKTEIDPLQPKVTVVGNVDPKILIKKLQRCGKQAEIWSSGNQNSGKQN 84

BLAST of MS018688 vs. NCBI nr
Match: XP_010660640.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 32 [Vitis vinifera] >CBI21235.3 unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-10
Identity = 34/50 (68.00%), Postives = 41/50 (82.00%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNNFNQIN 54
          GVL+ EIDPLQPKV++ GNVDP+ILIKKL + GKQA+IWS GN N  + N
Sbjct: 35 GVLKTEIDPLQPKVTVVGNVDPKILIKKLQRCGKQAEIWSSGNQNAGKQN 84

BLAST of MS018688 vs. NCBI nr
Match: XP_021657893.1 (heavy metal-associated isoprenylated plant protein 35-like [Hevea brasiliensis] >KAF2323011.1 hypothetical protein GH714_032740 [Hevea brasiliensis])

HSP 1 Score: 75.5 bits (184), Expect = 1.6e-10
Identity = 33/45 (73.33%), Postives = 41/45 (91.11%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNN 49
          GVL+ EIDPLQPKV++ GNVDPQILI++L KAGKQA++WSQG+ N
Sbjct: 35 GVLKTEIDPLQPKVTVLGNVDPQILIRRLLKAGKQAEMWSQGSQN 79

BLAST of MS018688 vs. NCBI nr
Match: XP_031288240.1 (heavy metal-associated isoprenylated plant protein 35-like [Pistacia vera])

HSP 1 Score: 75.1 bits (183), Expect = 2.1e-10
Identity = 34/45 (75.56%), Postives = 39/45 (86.67%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNN 49
          GVL+ EIDPLQPKV+  GNVDP+ILIKKL KAGKQA++WS GN N
Sbjct: 36 GVLKTEIDPLQPKVTALGNVDPKILIKKLLKAGKQAELWSNGNQN 80

BLAST of MS018688 vs. ExPASy Swiss-Prot
Match: Q9M8K5 (Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX=3702 GN=HIPP32 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 7.7e-08
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWS--QGNNNFNQ 52
          +  GV   +ID  Q KV++ G+VDP +LIKKL K+GK A+IW   +GNNN NQ
Sbjct: 33 KIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGKHAEIWGAPKGNNNPNQ 85

BLAST of MS018688 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.9e-07
Identity = 27/56 (48.21%), Postives = 36/56 (64.29%), Query Frame = 0

Query: 1   EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIW--SQGNNNFNQINY 55
           +  GV    ID  Q KV++ GNV+P+ILIKK+ KAG+ A++W  S  NN  N  NY
Sbjct: 53  KIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAELWPTSMENNINNDCNY 108

BLAST of MS018688 vs. ExPASy Swiss-Prot
Match: F4JZL7 (Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX=3702 GN=HIPP33 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 8.5e-07
Identity = 28/57 (49.12%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQ----GNNNFNQIN 54
          +  GV   +ID    KV++ GNVDP +LIKKL K+GK A+IW       NNN NQ N
Sbjct: 33 KIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGKHAEIWGAPKGGSNNNQNQPN 89

BLAST of MS018688 vs. ExPASy Swiss-Prot
Match: Q9C7J6 (Heavy metal-associated isoprenylated plant protein 35 OS=Arabidopsis thaliana OX=3702 GN=HIPP35 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.0e-04
Identity = 19/37 (51.35%), Postives = 29/37 (78.38%), Query Frame = 0

Query: 4   GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQ 41
           GV +++ID  Q KV++ G + P+IL+KKL+KAGK A+
Sbjct: 66  GVFKVDIDVKQHKVTVIGIISPEILLKKLNKAGKNAE 102

BLAST of MS018688 vs. ExPASy Swiss-Prot
Match: Q0WV37 (Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX=3702 GN=HIPP34 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 1.8e-04
Identity = 19/45 (42.22%), Postives = 30/45 (66.67%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQG 46
          +  GV  ++ D  Q +V++ GN+DP +L+KKL K+GK A+I   G
Sbjct: 33 KIEGVYSVKADVEQGRVTVTGNIDPALLVKKLSKSGKHAEILGGG 77

BLAST of MS018688 vs. ExPASy TrEMBL
Match: D7SVD6 (HMA domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0068g00670 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-11
Identity = 34/50 (68.00%), Postives = 41/50 (82.00%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNNFNQIN 54
          GVL+ EIDPLQPKV++ GNVDP+ILIKKL + GKQA+IWS GN N  + N
Sbjct: 35 GVLKTEIDPLQPKVTVVGNVDPKILIKKLQRCGKQAEIWSSGNQNAGKQN 84

BLAST of MS018688 vs. ExPASy TrEMBL
Match: A0A438EBL1 (Heavy metal-associated isoprenylated plant protein 32 OS=Vitis vinifera OX=29760 GN=HIPP32_5 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-11
Identity = 34/50 (68.00%), Postives = 41/50 (82.00%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNNFNQIN 54
          GVL+ EIDPLQPKV++ GNVDP+ILIKKL + GKQA+IWS GN N  + N
Sbjct: 35 GVLKTEIDPLQPKVTVVGNVDPKILIKKLQRCGKQAEIWSSGNQNSGKQN 84

BLAST of MS018688 vs. ExPASy TrEMBL
Match: A0A2C9W147 (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_04G096100 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-11
Identity = 34/46 (73.91%), Postives = 41/46 (89.13%), Query Frame = 0

Query: 3  SGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNN 49
          SGVL+ EID LQPKV++ GNVDPQILI+KL KAGKQA++WSQG+ N
Sbjct: 12 SGVLKTEIDTLQPKVTVLGNVDPQILIRKLLKAGKQAELWSQGSQN 57

BLAST of MS018688 vs. ExPASy TrEMBL
Match: A0A6A6NA12 (HMA domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_032740 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 7.7e-11
Identity = 33/45 (73.33%), Postives = 41/45 (91.11%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNNN 49
          GVL+ EIDPLQPKV++ GNVDPQILI++L KAGKQA++WSQG+ N
Sbjct: 35 GVLKTEIDPLQPKVTVLGNVDPQILIRRLLKAGKQAEMWSQGSQN 79

BLAST of MS018688 vs. ExPASy TrEMBL
Match: W9QM72 (HMA domain-containing protein OS=Morus notabilis OX=981085 GN=L484_007580 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-10
Identity = 34/44 (77.27%), Postives = 39/44 (88.64%), Query Frame = 0

Query: 4  GVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQGNN 48
          GV R+EIDPLQPKV++ GNVDPQILIKKL KAGKQA++WSQ  N
Sbjct: 35 GVYRMEIDPLQPKVTVVGNVDPQILIKKLSKAGKQAEVWSQLGN 78

BLAST of MS018688 vs. TAIR 10
Match: AT3G06130.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 5.4e-09
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWS--QGNNNFNQ 52
          +  GV   +ID  Q KV++ G+VDP +LIKKL K+GK A+IW   +GNNN NQ
Sbjct: 33 KIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGKHAEIWGAPKGNNNPNQ 85

BLAST of MS018688 vs. TAIR 10
Match: AT3G06130.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 5.4e-09
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWS--QGNNNFNQ 52
          +  GV   +ID  Q KV++ G+VDP +LIKKL K+GK A+IW   +GNNN NQ
Sbjct: 33 KIEGVFTTKIDSEQGKVTVSGSVDPSVLIKKLAKSGKHAEIWGAPKGNNNPNQ 85

BLAST of MS018688 vs. TAIR 10
Match: AT5G27690.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.1e-08
Identity = 27/56 (48.21%), Postives = 36/56 (64.29%), Query Frame = 0

Query: 1   EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIW--SQGNNNFNQINY 55
           +  GV    ID  Q KV++ GNV+P+ILIKK+ KAG+ A++W  S  NN  N  NY
Sbjct: 53  KIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAELWPTSMENNINNDCNY 108

BLAST of MS018688 vs. TAIR 10
Match: AT5G19090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.0e-08
Identity = 28/57 (49.12%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQ----GNNNFNQIN 54
          +  GV   +ID    KV++ GNVDP +LIKKL K+GK A+IW       NNN NQ N
Sbjct: 33 KIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGKHAEIWGAPKGGSNNNQNQPN 89

BLAST of MS018688 vs. TAIR 10
Match: AT5G19090.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.0e-08
Identity = 28/57 (49.12%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 1  EFSGVLRIEIDPLQPKVSIFGNVDPQILIKKLHKAGKQAQIWSQ----GNNNFNQIN 54
          +  GV   +ID    KV++ GNVDP +LIKKL K+GK A+IW       NNN NQ N
Sbjct: 33 KIEGVFTTKIDAELGKVTVSGNVDPSVLIKKLLKSGKHAEIWGAPKGGSNNNQNQPN 89

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_034708189.15.5e-1170.00heavy metal-associated isoprenylated plant protein 32-like [Vitis riparia][more]
RVW45064.11.2e-1068.00Heavy metal-associated isoprenylated plant protein 32 [Vitis vinifera][more]
XP_010660640.11.2e-1068.00PREDICTED: heavy metal-associated isoprenylated plant protein 32 [Vitis vinifera... [more]
XP_021657893.11.6e-1073.33heavy metal-associated isoprenylated plant protein 35-like [Hevea brasiliensis] ... [more]
XP_031288240.12.1e-1075.56heavy metal-associated isoprenylated plant protein 35-like [Pistacia vera][more]
Match NameE-valueIdentityDescription
Q9M8K57.7e-0852.83Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX... [more]
Q84J882.9e-0748.21Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX... [more]
F4JZL78.5e-0749.12Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX... [more]
Q9C7J61.0e-0451.35Heavy metal-associated isoprenylated plant protein 35 OS=Arabidopsis thaliana OX... [more]
Q0WV371.8e-0442.22Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
D7SVD65.9e-1168.00HMA domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_14s0068g00670 PE... [more]
A0A438EBL15.9e-1168.00Heavy metal-associated isoprenylated plant protein 32 OS=Vitis vinifera OX=29760... [more]
A0A2C9W1475.9e-1173.91Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_04G096100 PE=4 SV=... [more]
A0A6A6NA127.7e-1173.33HMA domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_032740 PE=4... [more]
W9QM721.0e-1077.27HMA domain-containing protein OS=Morus notabilis OX=981085 GN=L484_007580 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G06130.15.4e-0952.83Heavy metal transport/detoxification superfamily protein [more]
AT3G06130.25.4e-0952.83Heavy metal transport/detoxification superfamily protein [more]
AT5G27690.12.1e-0848.21Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.16.0e-0849.12Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.26.0e-0849.12Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 1..48
e-value: 1.8E-6
score: 29.9
NoneNo IPR availablePANTHERPTHR45868:SF14OS08G0205500 PROTEINcoord: 3..48
NoneNo IPR availablePANTHERPTHR45868HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATEDcoord: 3..48
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 4..39
e-value: 4.60818E-4
score: 32.1925
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 2..43

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018688.1MS018688.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding