MS018678 (gene) Bitter gourd (TR) v1

Overview
NameMS018678
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncalumenin-like
Locationscaffold313: 978351 .. 984445 (-)
RNA-Seq ExpressionMS018678
SyntenyMS018678
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAAAGTTTCCATTATCATATACATTACAGTAGCCTTACTTCTTCTCCTACTCGTTTCCCATTCCCCCAAGAAAACCCCAAATCACCGCCACCGCCGCCTCAAGCTTCGCTCCAATTTCACTTTTGCCCCCTCGCATCAGCACCGCGAGGCTATCCCACTCGACCCTCTCGTCGCCGACATTGAGCGCCGCCGTGAGGACCGGCAATGGGAGAAGCAATATGTGGAACATCACCATCCTGAGGTGGCCGCCCATTTGACGGAACCGGCCCCTGGTGAAGAGTCGCAGCCCGAGTGGGAGGACTTCGCGGATGCTGAGGATTATCTCAATGATGATAATAGGTTCAATGTGACTGATCGGCTGTCGTTGCTGTTTCCGAAGATTGATGTTCATCCAGCTGATGGGTTTGTCGATGTGGATGAGTTGACTGAGTGGAATCTGCAGCAGGCTGAGAGGGAAACTTTGCATAGGACTCAGAGGGAGATGGAGACGCACGATAAGAATCATGATGGGCTTGTTTCGTTTTCGGAGTACGAGCCTCCCAGCTGGATTCGAAATTCAGGTCTAATCTCCTTTAAGTGCATTTTGATTTTAGTTTTGTGTACTTGAAATTGGTGAGTCTGATGTTTTTCGTTTATTTTTCTTGTCGTGTTCAAAATCTTGCTCGTTGGAAATTGTTATTACTTATTTAGAAACTCGATATGTTGTATATTTAACAATCTCAAGGACCAAGAGTCGGGAATATTCTAAGATCGATTTCTTAAGGAAAAAGTATTTTGATAAAAACGAAATGTAATTTGCCGCACTTATGATGTTGTATGTAGATTCGTTGCAGTTGATGTTGTATGTAGATTCGTTGCAGTTTTTCCATTTGTCTTATGTTTGCTTTTTGGACACTTATTTTCTGTGTTACTCATTACCAAACTATTCACCAGATAATAGTTCCTTTGGCTACGATATGGGTTGGTGGAAACTTGGACATTTTAACGCGTCCGATGCTGATGGAGATGGTTTTTTGAATTTGACTGAGTTCAATGAGTAAGCTTTTCAAGATTTGTTGTGGTTATTCTTGAGGAGAATATGGAAAGTTTATTGGGTAGTTGAATTGAGATGCTCATAACGTTTATTCATCTCTGTTTTTTTAGCTTTCTGCACCCTGCTGACACCAAGAACCCAAAGCTACTTCATTGGCTGTGTGAGGAAGAAATACGGTAATGAATTATATGAAGTTATGTTCTTAACAGTTCTCAAAATCCTGCTTGATGTTTTAGGTGCATTTCCATTGGTTTCTATGGCTTTGTTTCCACTGCATTTGTCTTATAACGTTCAGTTGCCTAGTTGTTCTTCATCTCACCAATGGAGATTCTTGCTGAGGGCATTAACATCTTGTTTAACTGAAATAAACAAATTTTGGGGCAATTCTTATTTGCATAAACAATATTTTTGGGTTAATTCTTATTTCTGTAGATCTCAAAAACACCTTAACCTTTACACACTGCATTTGTACACATCTACTTATGTGTACAAAAGTGTTTACAAAAGCCTTTTGGAACAATTTTTTTTTCTTTTTTATAAGACCTTTTTGGAACTATTCAATCCAGGAAATTATTTTTGGGTCGAAGGTATTTTTGTAAAAAGGTGTTAGTTAGTATTGTTTTTATTTATGGGGTTACATTGTTATGTAAGTTCATAGAGAGATCGATATCTCGTGGTTTTGGAGTTTGTTACGTGTATAGTCTTTAATTTTTGGGTTTTACCTTGTAGGCTTGTTATAGGAGTTTGGTTTCGTGTAACTTTTCAAATATCAATGAAAAGTTTATGTTTATTGTCAAAAAGGGAAAAAAAAAAGTATTTTATACTCTTGTGCTGGCACAAGGGAAGATTGGATTGAACATTCAATTCTATAATATTCGGCTAAAATCACAACCGAAGAAGAGATGCTGTTATTATCCTCAACCTTCTTACAAAGGTATTTTTTTTGAACAAGATACGAACTTTTCATTGATATATGAAAAGGAACAAAATTGTTCAAAGATACAAACTCCCAACCTTCTTACAAAGGTATTAGCATCCCAAATACAAGAACCTAACAAGATAATTATATTGCAATCTCAAAAGATAAATTACAATATCGATAGCCTTTCGAGAGGGCTATCTCTCTCCCGAGAAACTCTCTCAAGTCCTCACCAAAATACTATTCCAAAAGATGATCTTCTCCTCTCCCTACTATCCTTATTTATAACAACTCACCCCTAACTAATTACCACATTGCCCCTTCACTAACATACTAACAATACATACCCCCATTAGGACTCTCACAAAAGGCTGCACATATTAGGGAAAATGTAAAGATGAGTATTCCTTCTTTGGACAACTTCAGCTATAATAAGGCCTCCTAGAGCTAAACTGCCAACAAAAATGTGACCTGTTAAGAAGCCTTGAATAATCTCCAAATTACTCGGTTTTAGTACTTTTTGGAAAAACTAGTTGGATATTTATAGCCTCATTAATTGTATAGATGAAAGATTTGGTGGAAAAGATGTCTGATGAATCTCCTTCCCTAATTCGAGAGTTTGCTTGGTAGGAAGGGTGGAAGTCTCTGAAGCACGAAATCAGCTGCAACCACTCTTCAATTTCTCTTTGGTTTAAGGCTCTTAAAAGTAAGGCATCCACTAGCATTCTTCTGCATCCCACTATCTCTACATTATTGTACATTTTTCAAGGAATTATCCCCGATTCGGACAGCTTCCCACTATTTAACTTGGTGCCATTTAATTTCCCGATAACAAACATATAAGATATCATTTAGAGGACAAAACTAGAGAAGAGGTTGATAAGAAATCAATCCATAAGTATTACTCTCATTTGTTCGAATGGGGATGTATTTATTTTCTCACCATAGTTTGTTTTTCTTCCTTTTCACTCTCTTTGGAGATACACCTTTGAAGTTTGTATTTTTGAACATTTTTTTTTGTTATTCATTGAATCAATGAAAAGTTCGTACTTGGTTAAAAATAAGAGAAGAAGAAAAAGAAATCAATCCATTAGTCAAAGAGCTCAATGGGGATTTCAAATAGCCTCTCCAATAGGTGTTGATAAAAAAAGGAATCGTCGCTACATAATAATTTAGCTGTTGCACTCCAAAAGGAAGCTTTGGCTTCCCAAATCTACTTCTCTCTTCTCTAGCCTTGTTAAGGAAATTTTATTTTTCTTTCAAGTCAGATCTCACAAATAGGAGCTTTGATCGTTTTCTCCCAAAGAATCTTGGCTTTTCTTTTCAAAAAGTTGGATCAAATATTGCTGACCTGTTAACTCGACTTCAGAGGGGATAGTTAGGTTACATTTCATAACCCTTCCGGAGTTCGGTTACTTCAACAAGGGAGACCCTTTTCTCTGTAACTTTCTGTAGCCGCACAACTCCTTCAATTCTAGTCTTCCTAAAGAGTTTCTGGTGTATTTGACTTCAAATTAGGATTTCAATTGCCTTTTATGTTCCCTGCATTTAGTCCAAGTCAAAAGCAAGGTGAAAGAACAACCAATTATTTTTTTCTTCAATTTTCTCTTCTAAATATTTGATCCTCACACAATTTTGTTGAAAAGAACTCCCTATATATTCTGCATTCCTCATGTTCATGTGAGAAACTCGATCCCCTTCCTAGTCGGAGAAGTCATTGAAGGTCTTGCTAGTCAGGTAGCATTTAAGAGAGATAATATTTAAAGCATCGTTAAATGTTACCTCATACCTGAACGATCAGGGAGATTCGGGAGGTGTTGTTGGAGAGAAATCTGAACTTTTGGGATAAGCATGTAATTTTGAACTACTATATTTAGTGGCTTTCTTTTTTAGGAATATTATTCATGAATGTAACAGAGTTGATGTGGTCAATTTTGATTTGTTTTAGCACTGCATTTCCAAAGTTTATTCTCATCAATATAAACGGATTTGCGATGTGGCTATGTCTCCATTTTTGTATCTGTTCAGGATATAATTTTCAATATTCCTTTTAATGTTTTTAATATATTAAAAATAATTATAGATTAGATTTTATGTTTTTACAAAAATTAGTGATATATAAAAGGTCATATTCTTATATGTTGTTTCAATTTGTTGATTAGCATAAACATATTGTACAAAATTAATATATTATATACTAAAACCATTAATTGCGTTCTTTATACATGCATGTTGAAATATATGTATGTATGTGTTAAGACACAATATAATAGTCAGAAGGAATATACAACATCAGAGGGGAGGTGGAAAAAAATCCACAAAAGCCATAATCCAAAGGTGAATACCAAAACGGTCTCCCCATTTGGTATAAAGCCAGAAAGGGAGTAAATACAAAGATGTTTGGACAAGGAAATTGAACAAGAGGCTAAGAAACAACCTGAATCAGAGATTTTTTTTTTTTTTTCCCTAGTTCAACGACATATGAGGGTGGAGATTTGAACCTCCAAACTTTTAGTCGAGGATATATGCTTTAACCAGTTAAGTTATGCTCAAGTTGGCAACTTGAATCAAAACTCTTATTAACTCCACTACTTTTGTCCTCAAATATTGTACTTTTTCTCTCAAAGTAAATCTCTAAAGAAAAAAGCCTCTGATGGTTTTGCACCCAGTAACTATAGCTCTACTGGAGAAAACTGGCCAAATCAGTACCTTAGAGATGATACAGTTACACTTGTGAAGCAAAATTTTTTAAACAAATACCATATTTTTTTGAAAGTGGACAGTGAAGAACAGGTGTTCTAGACTTCCAATATGGTTGGGTTGAAGCTCAAAAGCACACCAGTTAAGATGTAGGCATAGTGGCATACAAGCATTGGGGAGCTTTATATGCTCTCTATTAATATCCACAAGAAAAAATTAACCTTAAGAGTATTTGTTTTTACTTTTTCCTAGCCATCTTTGTAGCTCTACTAGAGATCTCTAGATTCTAGGAACTAGGAAGTTGAGAAGGGACTTTGTAGTAAATTTCTAGAAGAATTGGGAGTCATATAGTCTGATGTTACTGTCTATCATTGGGCCTAAAATCTTCCACTACTTGGAGAGGAGATCCCATTCTACGATAAGAAGCACGGACACTTTAGTTTGGGTAGACACTTGGACACTTATCTCTCAATAGATGTGTGTCACACACAAGTTATACAAAGTGAATATGGATCCAATACTTGTTAGGCACATATTAGACACATGTTAAACATAAGAAATAGACATAAGTAGTAATATAAGGACAACGTAATAATTTTTTGCGAGCAAAATACATCAAACTCATTTTCTAAGCACGAAAATGCATAAGTTTGTTGACTTTGATATCCTTTTGGTATAAAAATGATATATACTTACGAACATGGTATATTCTAATAAACACATCCTTGTCGTATCCGTGTCCTAGATTTTTAGAAAATGATGTGTCACCTTGTCCGTATCGCACCCGTGTCTCGTGTCCATATATGTTTTCGTGATTCTTAGACTCTGTGAGAAATAAATATTATAATCTTATTGTATGTGATGGGTATTTTGTTATCATAAGTAAATTTAATACCACTCTCGAGAAGATTTTATGATGGAAATATCAAATATATTAAGATCCCAAATATACTCTTTGGATCTCTCCATCCTCCAAAAATTCTATTGTTTCTGCGAGCCATATTCCCCATAAAGTAGGGGTCTCTCATTTCAGTAGCTTTGCTAACTAGTTAATTGGTGAGTTGCCTTGTAGGGAGAGAGATTCAGACAAGGATGGGAAGATCAACTTCAATGAGTTTTTCCATGGACTCTTTGACTTGGTGAGAAATTATGATGAGAATCACCACTCTTCACATGAGTCAGATGATCCGAGGGATGGCCCTGCTAGGAATTTGTTTGCGGTGCTAGACAAAGATAACGACGGGTAATTTAAGTTTATGATGAGGTCAACGTCATACTTGGTTAACTGCAGAGTTTCTTGGCACCTTTAATGATTTGTTTTCGTTATTTTGTTTTTCATAGGCACCTGTCCGATGAAGAGTTGTTACCTATAATTGGAAAAATCCACCCATCAGAGCATTACTATGCGAAACAGCAAGCAGAATATATCGTACAGCAG

mRNA sequence

ATGAGCAAAGTTTCCATTATCATATACATTACAGTAGCCTTACTTCTTCTCCTACTCGTTTCCCATTCCCCCAAGAAAACCCCAAATCACCGCCACCGCCGCCTCAAGCTTCGCTCCAATTTCACTTTTGCCCCCTCGCATCAGCACCGCGAGGCTATCCCACTCGACCCTCTCGTCGCCGACATTGAGCGCCGCCGTGAGGACCGGCAATGGGAGAAGCAATATGTGGAACATCACCATCCTGAGGTGGCCGCCCATTTGACGGAACCGGCCCCTGGTGAAGAGTCGCAGCCCGAGTGGGAGGACTTCGCGGATGCTGAGGATTATCTCAATGATGATAATAGGTTCAATGTGACTGATCGGCTGTCGTTGCTGTTTCCGAAGATTGATGTTCATCCAGCTGATGGGTTTGTCGATGTGGATGAGTTGACTGAGTGGAATCTGCAGCAGGCTGAGAGGGAAACTTTGCATAGGACTCAGAGGGAGATGGAGACGCACGATAAGAATCATGATGGGCTTGTTTCGTTTTCGGAGTACGAGCCTCCCAGCTGGATTCGAAATTCAGATAATAGTTCCTTTGGCTACGATATGGGTTGGTGGAAACTTGGACATTTTAACGCGTCCGATGCTGATGGAGATGGTTTTTTGAATTTGACTGAGTTCAATGACTTTCTGCACCCTGCTGACACCAAGAACCCAAAGCTACTTCATTGGCTGTGTGAGGAAGAAATACGGGAGAGAGATTCAGACAAGGATGGGAAGATCAACTTCAATGAGTTTTTCCATGGACTCTTTGACTTGGTGAGAAATTATGATGAGAATCACCACTCTTCACATGAGTCAGATGATCCGAGGGATGGCCCTGCTAGGAATTTGTTTGCGGTGCTAGACAAAGATAACGACGGGCACCTGTCCGATGAAGAGTTGTTACCTATAATTGGAAAAATCCACCCATCAGAGCATTACTATGCGAAACAGCAAGCAGAATATATCGTACAGCAG

Coding sequence (CDS)

ATGAGCAAAGTTTCCATTATCATATACATTACAGTAGCCTTACTTCTTCTCCTACTCGTTTCCCATTCCCCCAAGAAAACCCCAAATCACCGCCACCGCCGCCTCAAGCTTCGCTCCAATTTCACTTTTGCCCCCTCGCATCAGCACCGCGAGGCTATCCCACTCGACCCTCTCGTCGCCGACATTGAGCGCCGCCGTGAGGACCGGCAATGGGAGAAGCAATATGTGGAACATCACCATCCTGAGGTGGCCGCCCATTTGACGGAACCGGCCCCTGGTGAAGAGTCGCAGCCCGAGTGGGAGGACTTCGCGGATGCTGAGGATTATCTCAATGATGATAATAGGTTCAATGTGACTGATCGGCTGTCGTTGCTGTTTCCGAAGATTGATGTTCATCCAGCTGATGGGTTTGTCGATGTGGATGAGTTGACTGAGTGGAATCTGCAGCAGGCTGAGAGGGAAACTTTGCATAGGACTCAGAGGGAGATGGAGACGCACGATAAGAATCATGATGGGCTTGTTTCGTTTTCGGAGTACGAGCCTCCCAGCTGGATTCGAAATTCAGATAATAGTTCCTTTGGCTACGATATGGGTTGGTGGAAACTTGGACATTTTAACGCGTCCGATGCTGATGGAGATGGTTTTTTGAATTTGACTGAGTTCAATGACTTTCTGCACCCTGCTGACACCAAGAACCCAAAGCTACTTCATTGGCTGTGTGAGGAAGAAATACGGGAGAGAGATTCAGACAAGGATGGGAAGATCAACTTCAATGAGTTTTTCCATGGACTCTTTGACTTGGTGAGAAATTATGATGAGAATCACCACTCTTCACATGAGTCAGATGATCCGAGGGATGGCCCTGCTAGGAATTTGTTTGCGGTGCTAGACAAAGATAACGACGGGCACCTGTCCGATGAAGAGTTGTTACCTATAATTGGAAAAATCCACCCATCAGAGCATTACTATGCGAAACAGCAAGCAGAATATATCGTACAGCAG

Protein sequence

MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ
Homology
BLAST of MS018678 vs. NCBI nr
Match: XP_022141578.1 (calumenin-like [Momordica charantia] >XP_022141579.1 calumenin-like [Momordica charantia])

HSP 1 Score: 689.1 bits (1777), Expect = 1.9e-194
Identity = 333/334 (99.70%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVA 60
           MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIP DPLVA
Sbjct: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPFDPLVA 60

Query: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120
           DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD
Sbjct: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120

Query: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180
           RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE
Sbjct: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180

Query: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240
           PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC
Sbjct: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240

Query: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300
           EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN
Sbjct: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300

Query: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ
Sbjct: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 334

BLAST of MS018678 vs. NCBI nr
Match: XP_022939873.1 (calumenin-like [Cucurbita moschata] >KAG6579084.1 Calumenin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 631.7 bits (1628), Expect = 3.6e-177
Identity = 304/338 (89.94%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPS----HQHREAIPLD 60
           MSKVSII+YITVA+LLLLLVSHSPKK+PN RHRRLKLRSNFTFAPS    H H EA+P D
Sbjct: 1   MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF 120
           PLVADIERRREDRQWEKQYVE HHPE+AAHLTE APGEESQPEWEDFADAEDYLNDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDDNRF 120

Query: 121 NVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSF 180
           NVTDRL+LLFPKIDVHP+DGFV VDELTEWNLQQA+RETLHRTQRE+ETHDKNHDGLVSF
Sbjct: 121 NVTDRLTLLFPKIDVHPSDGFVAVDELTEWNLQQAQRETLHRTQRELETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLL 240
           SEYEPPSW RNSDNSSFG++MGWWK  HFNASDADGDG LNLTEFNDFLHPAD+KNPKLL
Sbjct: 181 SEYEPPSWARNSDNSSFGFNMGWWKFEHFNASDADGDGLLNLTEFNDFLHPADSKNPKLL 240

Query: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVL 300
           HWLCEEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVL
Sbjct: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVL 300

Query: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DKDNDGHLSDEELLPIIGKIHPSEHYYA+QQAEYI+QQ
Sbjct: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAEQQAEYIIQQ 338

BLAST of MS018678 vs. NCBI nr
Match: KAG7016609.1 (Calumenin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 631.7 bits (1628), Expect = 3.6e-177
Identity = 304/338 (89.94%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPS----HQHREAIPLD 60
           MSKVSII+YITVA+LLLLLVSHSPKK+PN RHRRLKLRSNFTFAPS    H H EA+P D
Sbjct: 52  MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 111

Query: 61  PLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF 120
           PLVADIERRREDRQWEKQYVE HHPE+AAHLTE APGEESQPEWEDFADAEDYLNDDNRF
Sbjct: 112 PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDDNRF 171

Query: 121 NVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSF 180
           NVTDRL+LLFPKIDVHP+DGFV VDELTEWNLQQA+RETLHRTQRE+ETHDKNHDGLVSF
Sbjct: 172 NVTDRLTLLFPKIDVHPSDGFVAVDELTEWNLQQAQRETLHRTQRELETHDKNHDGLVSF 231

Query: 181 SEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLL 240
           SEYEPPSW RNSDNSSFG++MGWWK  HFNASDADGDG LNLTEFNDFLHPAD+KNPKLL
Sbjct: 232 SEYEPPSWARNSDNSSFGFNMGWWKFEHFNASDADGDGLLNLTEFNDFLHPADSKNPKLL 291

Query: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVL 300
           HWLCEEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVL
Sbjct: 292 HWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVL 351

Query: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DKDNDGHLSDEELLPIIGKIHPSEHYYA+QQAEYI+QQ
Sbjct: 352 DKDNDGHLSDEELLPIIGKIHPSEHYYAEQQAEYIIQQ 389

BLAST of MS018678 vs. NCBI nr
Match: XP_023550368.1 (calumenin-like [Cucurbita pepo subsp. pepo] >XP_023550369.1 calumenin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.3e-176
Identity = 303/338 (89.64%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPS----HQHREAIPLD 60
           MSKVSII+YITVA+LLLLLVSHSPKK+PN RHRRLKLRSNFTFAPS    H H EA+P D
Sbjct: 1   MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF 120
           PLVADIERRREDRQWEKQYVE HHPE+AAHLTE APGEESQPEWEDFADAEDYLNDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDDNRF 120

Query: 121 NVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSF 180
           NVTDRL+LLFPKIDVHP+DGFV V ELTEWNLQQA+RETLHRTQRE+ETHDKNHDGLVSF
Sbjct: 121 NVTDRLTLLFPKIDVHPSDGFVAVHELTEWNLQQAQRETLHRTQRELETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLL 240
           SEYEPPSW RNSDNSSFG++MGWWK  HFNASDADGDG LNLTEFNDFLHPAD+KNPKLL
Sbjct: 181 SEYEPPSWARNSDNSSFGFNMGWWKFEHFNASDADGDGLLNLTEFNDFLHPADSKNPKLL 240

Query: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVL 300
           HWLCEEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVL
Sbjct: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVL 300

Query: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DKDNDGHLSDEELLPIIGKIHPSEHYYA+QQAEYI+QQ
Sbjct: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAEQQAEYIIQQ 338

BLAST of MS018678 vs. NCBI nr
Match: XP_038876256.1 (calumenin-like [Benincasa hispida])

HSP 1 Score: 628.6 bits (1620), Expect = 3.0e-176
Identity = 298/334 (89.22%), Postives = 317/334 (94.91%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVA 60
           MSKVSI+IYITV++LLLLLVSHSP KTPNHRHRRLKLRSNFTFAPSH H E +P DPLVA
Sbjct: 1   MSKVSILIYITVSILLLLLVSHSPNKTPNHRHRRLKLRSNFTFAPSHHHHEPVPFDPLVA 60

Query: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120
           DIERRREDRQWEKQYVE H+P++AA L EPAPGEESQPEWEDFADAEDYLNDDNRFNVTD
Sbjct: 61  DIERRREDRQWEKQYVEQHYPKMAARLMEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120

Query: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180
           RL+LLFPKIDVHP DGFV VDELTEWNLQQA+RETLHRTQRE+ETHDKNHDG+VSFSEYE
Sbjct: 121 RLTLLFPKIDVHPVDGFVTVDELTEWNLQQAQRETLHRTQRELETHDKNHDGVVSFSEYE 180

Query: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240
           PPSW+RNSDN+SFGYDMGWWK  HFNASD DGDG LNLTEFNDFLHPAD+KNPKLLHWLC
Sbjct: 181 PPSWVRNSDNNSFGYDMGWWKFEHFNASDVDGDGLLNLTEFNDFLHPADSKNPKLLHWLC 240

Query: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300
           EEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S++ RDG ARNLFAVLDKDN
Sbjct: 241 EEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEESRDGLARNLFAVLDKDN 300

Query: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DGHLSDEELLPIIGKIHPSEHYYAKQQAEYI+QQ
Sbjct: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIIQQ 334

BLAST of MS018678 vs. ExPASy Swiss-Prot
Match: Q6XLQ7 (Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 4.1e-11
Identity = 71/270 (26.30%), Postives = 121/270 (44.81%), Query Frame = 0

Query: 72  EKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF--NVTDRLSLLFPKI 131
           EK+   HH P+++  + + A  +    + + F  AE+    D        +RL  +  KI
Sbjct: 23  EKKDRVHHEPQLSDKVHDDA--QSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKI 82

Query: 132 DVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYEPPS--WIRN 191
           D    DGFV VDEL +W     +R      +R+ + HD N DGLVS+ EY+  +  ++ +
Sbjct: 83  DA-DKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLD 142

Query: 192 SDNSSFGYDMGWWKL---GHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLCEEEI 251
             +   G++     +     F  +D DGD      EF  FLHP +    K +  + +E +
Sbjct: 143 DPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDI--VVQETM 202

Query: 252 RERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGHL 311
            + D + DG I+  E+   ++    N DE      E +              DK+ DG +
Sbjct: 203 EDIDKNGDGFIDLEEYIGDMYSHDGNADEPEWVKTERE--------QFVEFRDKNRDGKM 262

Query: 312 SDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
             EE       I PS++ +A+ +A ++V +
Sbjct: 263 DKEETK---DWILPSDYDHAEAEARHLVYE 276

BLAST of MS018678 vs. ExPASy Swiss-Prot
Match: O35887 (Calumenin OS=Mus musculus OX=10090 GN=Calu PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.1e-11
Identity = 72/271 (26.57%), Postives = 125/271 (46.13%), Query Frame = 0

Query: 72  EKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF--NVTDRLSLLFPKI 131
           EK+   HH P+++  +   A  +    + + F  AE+  + D        +RL  +  KI
Sbjct: 23  EKKDRVHHEPQLSDKVHNDA--QNFDYDHDAFLGAEEAKSFDQLTPEESKERLGKIVSKI 82

Query: 132 DVHPADGFVDVDELTEWNLQQAERETLHR-TQREMETHDKNHDGLVSFSEYEPPS--WIR 191
           D    DGFV VDEL  W ++ A++  +H   +R+ + HD N DGLVS+ EY+  +  ++ 
Sbjct: 83  D-DDKDGFVTVDELKGW-IKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVL 142

Query: 192 NSDNSSFGYDMGWWKL---GHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLCEEE 251
           +  +   G++     +     F  +D DGD      EF  FLHP +    K +  + +E 
Sbjct: 143 DDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDI--VVQET 202

Query: 252 IRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGH 311
           + + D + DG I+  E+   ++    N DE      E +              DK+ DG 
Sbjct: 203 MEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTERE--------QFVEFRDKNRDGK 262

Query: 312 LSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           +  EE       I PS++ +A+ +A ++V +
Sbjct: 263 MDKEETK---DWILPSDYDHAEAEARHLVYE 276

BLAST of MS018678 vs. ExPASy Swiss-Prot
Match: Q5RDD8 (Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 7.1e-11
Identity = 71/270 (26.30%), Postives = 120/270 (44.44%), Query Frame = 0

Query: 72  EKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF--NVTDRLSLLFPKI 131
           EK+   HH P+++  +   A  +    + + F  AE+    D        +RL  +  KI
Sbjct: 23  EKKDRVHHEPQLSDKVHNDA--QSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKI 82

Query: 132 DVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYEPPS--WIRN 191
           D    DGFV VDEL +W     +R      +R+ + HD N DGLVS+ EY+  +  ++ +
Sbjct: 83  D-DDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLD 142

Query: 192 SDNSSFGYDMGWWKL---GHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLCEEEI 251
             +   G++     +     F  +D DGD      EF  FLHP +    K +  + +E +
Sbjct: 143 DPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDI--VVQETM 202

Query: 252 RERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGHL 311
            + D + DG I+  E+   ++    N DE      E +              DK+ DG +
Sbjct: 203 EDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTERE--------QFVEFRDKNRDGKM 262

Query: 312 SDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
             EE       I PS++ +A+ +A ++V +
Sbjct: 263 DKEETK---DWILPSDYDHAEAEARHLVYE 276

BLAST of MS018678 vs. ExPASy Swiss-Prot
Match: O43852 (Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 9.2e-11
Identity = 71/270 (26.30%), Postives = 120/270 (44.44%), Query Frame = 0

Query: 72  EKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF--NVTDRLSLLFPKI 131
           EK+   HH P+++  +   A  +    + + F  AE+    D        +RL  +  KI
Sbjct: 23  EKKDRVHHEPQLSDKVHNDA--QSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKI 82

Query: 132 DVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYEPPS--WIRN 191
           D    DGFV VDEL +W     +R      +R+ + HD N DGLVS+ EY+  +  ++ +
Sbjct: 83  D-GDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLD 142

Query: 192 SDNSSFGYDMGWWKL---GHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLCEEEI 251
             +   G++     +     F  +D DGD      EF  FLHP +    K +  + +E +
Sbjct: 143 DPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDI--VVQETM 202

Query: 252 RERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGHL 311
            + D + DG I+  E+   ++    N DE      E +              DK+ DG +
Sbjct: 203 EDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTERE--------QFVEFRDKNRDGKM 262

Query: 312 SDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
             EE       I PS++ +A+ +A ++V +
Sbjct: 263 DKEETK---DWILPSDYDHAEAEARHLVYE 276

BLAST of MS018678 vs. ExPASy Swiss-Prot
Match: Q4U471 (Calumenin OS=Mesocricetus auratus OX=10036 GN=CALU PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.6e-10
Identity = 71/271 (26.20%), Postives = 124/271 (45.76%), Query Frame = 0

Query: 72  EKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF--NVTDRLSLLFPKI 131
           EK+   HH P+++  +   A  +    + + F  AE+  + D        +RL  +  KI
Sbjct: 23  EKKDRVHHEPQLSDKVHNDA--QNFDYDHDAFLGAEEAKSFDQLTPEESKERLGKIVSKI 82

Query: 132 DVHPADGFVDVDELTEWNLQQAERETLHR-TQREMETHDKNHDGLVSFSEYEPPS--WIR 191
           D    DGFV VDEL  W ++ A++  +H   +R+ + HD N DGLVS+ EY+  +  ++ 
Sbjct: 83  D-DDKDGFVTVDELKGW-IKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGYVL 142

Query: 192 NSDNSSFGYDMGWWKL---GHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLCEEE 251
           +  +   G++     +     F  +D DGD      EF  F HP +    K +  + +E 
Sbjct: 143 DDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFPHPDEYDYMKDI--VVQET 202

Query: 252 IRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGH 311
           + + D + DG I+  E+   ++    N DE      E +              DK+ DG 
Sbjct: 203 MEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTERE--------QFVEFRDKNRDGR 262

Query: 312 LSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           +  EE       I PS++ +A+ +A ++V +
Sbjct: 263 MDKEETK---DWILPSDYDHAEAEARHLVYE 276

BLAST of MS018678 vs. ExPASy TrEMBL
Match: A0A6J1CJ22 (calumenin-like OS=Momordica charantia OX=3673 GN=LOC111011909 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 9.1e-195
Identity = 333/334 (99.70%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVA 60
           MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIP DPLVA
Sbjct: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPFDPLVA 60

Query: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120
           DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD
Sbjct: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120

Query: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180
           RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE
Sbjct: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180

Query: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240
           PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC
Sbjct: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240

Query: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300
           EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN
Sbjct: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300

Query: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ
Sbjct: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 334

BLAST of MS018678 vs. ExPASy TrEMBL
Match: A0A6J1FMS7 (calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.7e-177
Identity = 304/338 (89.94%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPS----HQHREAIPLD 60
           MSKVSII+YITVA+LLLLLVSHSPKK+PN RHRRLKLRSNFTFAPS    H H EA+P D
Sbjct: 1   MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF 120
           PLVADIERRREDRQWEKQYVE HHPE+AAHLTE APGEESQPEWEDFADAEDYLNDDNRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDDNRF 120

Query: 121 NVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSF 180
           NVTDRL+LLFPKIDVHP+DGFV VDELTEWNLQQA+RETLHRTQRE+ETHDKNHDGLVSF
Sbjct: 121 NVTDRLTLLFPKIDVHPSDGFVAVDELTEWNLQQAQRETLHRTQRELETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLL 240
           SEYEPPSW RNSDNSSFG++MGWWK  HFNASDADGDG LNLTEFNDFLHPAD+KNPKLL
Sbjct: 181 SEYEPPSWARNSDNSSFGFNMGWWKFEHFNASDADGDGLLNLTEFNDFLHPADSKNPKLL 240

Query: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVL 300
           HWLCEEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVL
Sbjct: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVL 300

Query: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DKDNDGHLSDEELLPIIGKIHPSEHYYA+QQAEYI+QQ
Sbjct: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAEQQAEYIIQQ 338

BLAST of MS018678 vs. ExPASy TrEMBL
Match: A0A5D3CVB0 (Calumenin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00460 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.5e-176
Identity = 298/334 (89.22%), Postives = 316/334 (94.61%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVA 60
           MSKVSIIIYITVA+LLLLL+SHSPKKTPN RHRRLKLRSNFTF PSH H E +P DPLVA
Sbjct: 1   MSKVSIIIYITVAILLLLLLSHSPKKTPNQRHRRLKLRSNFTFTPSHHHHEPVPFDPLVA 60

Query: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120
           DIERRREDRQWEKQYVE H+P++AAHLTE APGEESQPEWEDFADAEDY+NDDNRFNVTD
Sbjct: 61  DIERRREDRQWEKQYVEQHYPKMAAHLTESAPGEESQPEWEDFADAEDYINDDNRFNVTD 120

Query: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180
           RL LLFPKIDV P DGFV V+ELTEWNLQQA+RETLHRTQRE+ETHDKNHDG VSFSEYE
Sbjct: 121 RLMLLFPKIDVDPVDGFVTVEELTEWNLQQAQRETLHRTQRELETHDKNHDGSVSFSEYE 180

Query: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240
           PPSW+RNSDNSSFGYDMGWWKL HFNASD DGDG LNLTEFNDFLHPAD+KNPKL+HWLC
Sbjct: 181 PPSWVRNSDNSSFGYDMGWWKLEHFNASDVDGDGLLNLTEFNDFLHPADSKNPKLIHWLC 240

Query: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300
           EEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVLDKDN
Sbjct: 241 EEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDN 300

Query: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DGHLS+EELLPIIGKIHPSEHYYAKQQAEYI+QQ
Sbjct: 301 DGHLSNEELLPIIGKIHPSEHYYAKQQAEYIIQQ 334

BLAST of MS018678 vs. ExPASy TrEMBL
Match: A0A1S3CNB9 (calumenin-B-like OS=Cucumis melo OX=3656 GN=LOC103502856 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 5.5e-176
Identity = 298/334 (89.22%), Postives = 316/334 (94.61%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVA 60
           MSKVSIIIYITVA+LLLLL+SHSPKKTPN RHRRLKLRSNFTF PSH H E +P DPLVA
Sbjct: 1   MSKVSIIIYITVAILLLLLLSHSPKKTPNQRHRRLKLRSNFTFTPSHHHHEPVPFDPLVA 60

Query: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120
           DIERRREDRQWEKQYVE H+P++AAHLTE APGEESQPEWEDFADAEDY+NDDNRFNVTD
Sbjct: 61  DIERRREDRQWEKQYVEQHYPKMAAHLTESAPGEESQPEWEDFADAEDYINDDNRFNVTD 120

Query: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180
           RL LLFPKIDV P DGFV V+ELTEWNLQQA+RETLHRTQRE+ETHDKNHDG VSFSEYE
Sbjct: 121 RLMLLFPKIDVDPVDGFVTVEELTEWNLQQAQRETLHRTQRELETHDKNHDGSVSFSEYE 180

Query: 181 PPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWLC 240
           PPSW+RNSDNSSFGYDMGWWKL HFNASD DGDG LNLTEFNDFLHPAD+KNPKL+HWLC
Sbjct: 181 PPSWVRNSDNSSFGYDMGWWKLEHFNASDVDGDGLLNLTEFNDFLHPADSKNPKLIHWLC 240

Query: 241 EEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDN 300
           EEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVLDKDN
Sbjct: 241 EEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDN 300

Query: 301 DGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DGHLS+EELLPIIGKIHPSEHYYAKQQAEYI+QQ
Sbjct: 301 DGHLSNEELLPIIGKIHPSEHYYAKQQAEYIIQQ 334

BLAST of MS018678 vs. ExPASy TrEMBL
Match: A0A6J1JYP4 (calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111489489 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.0e-174
Identity = 298/338 (88.17%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPS----HQHREAIPLD 60
           MSKVSII+YITVA+LLLLLVSHSPKK+PN RHRRLKLRSNFTFAPS    H H EA+P D
Sbjct: 1   MSKVSIIVYITVAVLLLLLVSHSPKKSPNRRHRRLKLRSNFTFAPSHHDDHHHHEAVPFD 60

Query: 61  PLVADIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRF 120
           PLVADIERRREDRQWEKQYVE HHPE+AAHLTE APGEESQPEWEDFADAEDYLND+NRF
Sbjct: 61  PLVADIERRREDRQWEKQYVEQHHPEMAAHLTEHAPGEESQPEWEDFADAEDYLNDNNRF 120

Query: 121 NVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSF 180
           NVTDRL+LLFPKIDVHP DGFV VDEL EWNLQQ +RETLHRTQRE+ETHDKNHDGLVSF
Sbjct: 121 NVTDRLTLLFPKIDVHPPDGFVAVDELIEWNLQQTQRETLHRTQRELETHDKNHDGLVSF 180

Query: 181 SEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLL 240
           SEYEPPSW+RNSDNSSFG++MGWWK  HFN SDADGDG LNLTEFNDFLHPAD+KNPKLL
Sbjct: 181 SEYEPPSWVRNSDNSSFGFNMGWWKFEHFNVSDADGDGLLNLTEFNDFLHPADSKNPKLL 240

Query: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVL 300
           HWLCEEEIRERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S++ RDGPARNLFAVL
Sbjct: 241 HWLCEEEIRERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEESRDGPARNLFAVL 300

Query: 301 DKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           DKDNDGHLSDEELLPIIGKIHPSE+YYA+QQAEYI+QQ
Sbjct: 301 DKDNDGHLSDEELLPIIGKIHPSENYYAEQQAEYIIQQ 338

BLAST of MS018678 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.1e-136
Identity = 234/351 (66.67%), Postives = 283/351 (80.63%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNH--------RHRRLKLRSNFTFAPSHQHREA 60
           MSK S+I+YITV +L+L LVS+SPKK  +H        +H RLKLRS+F F P+    + 
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFKPT--RHDP 60

Query: 61  IPLDPLVADIERRREDRQWEKQYVEHHHPEVAAHLT--------EPAPGEESQPEWEDFA 120
           +P DPLVAD+ERRRED++WE+QY+EH HPE+ +H          E APG ESQPEWE+F 
Sbjct: 61  VPFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFM 120

Query: 121 DAEDYLNDDNRFNVTDRLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREME 180
           DAEDYLND+ +FNVTDRL LLFPKIDV PADGF+   ELTEW +Q + +E +HRTQR+++
Sbjct: 121 DAEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLD 180

Query: 181 THDKNHDGLVSFSEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDF 240
            HD+N DG +SFSEYEPPSW+R SDN+SFGYDMGWWK  HFNASDA+GDG LNLTEFNDF
Sbjct: 181 VHDRNKDGFISFSEYEPPSWVRKSDNNSFGYDMGWWKEEHFNASDANGDGLLNLTEFNDF 240

Query: 241 LHPADTKNPKLLHWLCEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDE-NHHSSHESDD 300
           LHPADTKNPKLL WLC+EE+RERDSDKDGKI+F EFFHGLFD VRNY+E NH+S+H   D
Sbjct: 241 LHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYHD 300

Query: 301 PRDGPARNLFAVLDKDNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
             +GPA+ LF+ LDK++DG+LSD ELLPII KIHP+EHYYAKQQA+YI+ Q
Sbjct: 301 LPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQ 349

BLAST of MS018678 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 150.6 bits (379), Expect = 2.2e-36
Identity = 99/336 (29.46%), Postives = 164/336 (48.81%), Query Frame = 0

Query: 1   MSKVSIIIYITVALLLLLLVSHSPKKTPNHRHRRLKLRSNFTFAPSHQHREAIPLDPLVA 60
           M+KV +   +T  ++ L+L++H  +         +  R         +  E    DPLV 
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIG-------RRLEMPVFDPLVT 60

Query: 61  DIERRREDRQWEKQYVEHHHPEVAAHLTEPAPGEESQPEWEDFADAEDYLNDDNRFNVTD 120
            IER              H  E      E A  E+           E+Y   + R N T 
Sbjct: 61  RIERL------------SHEKEAGTKTVEAAKEEKDDM-------FEEYFAQERRLNTTM 120

Query: 121 RLSLLFPKIDVHPADGFVDVDELTEWNLQQAERETLHRTQREMETHDKNHDGLVSFSEYE 180
           R+  LFP +D  P DGFV + EL  W +QQ E   ++RT +E+E  DK+ DG+++F EY 
Sbjct: 121 RIKFLFPLLDASPRDGFVSLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYL 180

Query: 181 PPSWIRNSDNSSFGY-DMGWWKLGHFNASDADGDGFLNLTEFNDFLHPADTKNPKLLHWL 240
           P    ++ + +  G+ + GWW +  F  SD D +G L++ EFN+FLHP D++N     W+
Sbjct: 181 PQFSKQDIEKNEKGHGEAGWW-MEQFKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWV 240

Query: 241 CEEEIRERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPA-RNLFAVLDK 300
            +E +   D++ DGK+ + EF    +++ + +     +  E ++  + P  + LFA +D+
Sbjct: 241 LKERMTGMDTNGDGKLEYKEFVKNAYEMYKEF-----AKFEKEEDENVPTPQLLFAEMDR 300

Query: 301 DNDGHLSDEELLPIIGKIHPSEHYYAKQQAEYIVQQ 335
           D D  L  +EL PI+  + P E  YAK  + ++  +
Sbjct: 301 DKDRFLVADELRPILQYLQPGEMSYAKFYSTFLCHE 304

BLAST of MS018678 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-05
Identity = 33/96 (34.38%), Postives = 45/96 (46.88%), Query Frame = 0

Query: 167 DKNHDGLVSFSEYEP--PSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDF 226
           D N DG V  +E+E    S +   +      DM       FN  D DGDGF+ + E N  
Sbjct: 96  DANGDGCVDINEFESLYGSIVEEKEEG----DM----RDAFNVFDQDGDGFITVEELNSV 155

Query: 227 LHPADTKNPKLLHWLCEEEIRERDSDKDGKINFNEF 261
           +     K  K L   C+E I + D D DG++N+ EF
Sbjct: 156 MTSLGLKQGKTLE-CCKEMIMQVDEDGDGRVNYKEF 182

BLAST of MS018678 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 43.9 bits (102), Expect = 2.9e-04
Identity = 29/94 (30.85%), Postives = 43/94 (45.74%), Query Frame = 0

Query: 167 DKNHDGLVSFSEYEPPSWIRNSDNSSFGYDMGWWKLGHFNASDADGDGFLNLTEFNDFLH 226
           D N DG V   E+E        ++ + G          FN  D DGDGF+ + E    + 
Sbjct: 110 DANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMA 169

Query: 227 PADTKNPKLLHWLCEEEIRERDSDKDGKINFNEF 261
               K  K L   C++ I + D+D DG++N+ EF
Sbjct: 170 SLGLKQGKTLDG-CKKMIMQVDADGDGRVNYKEF 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141578.11.9e-19499.70calumenin-like [Momordica charantia] >XP_022141579.1 calumenin-like [Momordica c... [more]
XP_022939873.13.6e-17789.94calumenin-like [Cucurbita moschata] >KAG6579084.1 Calumenin, partial [Cucurbita ... [more]
KAG7016609.13.6e-17789.94Calumenin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023550368.12.3e-17689.64calumenin-like [Cucurbita pepo subsp. pepo] >XP_023550369.1 calumenin-like [Cucu... [more]
XP_038876256.13.0e-17689.22calumenin-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q6XLQ74.1e-1126.30Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2[more]
O358877.1e-1126.57Calumenin OS=Mus musculus OX=10090 GN=Calu PE=1 SV=1[more]
Q5RDD87.1e-1126.30Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=1[more]
O438529.2e-1126.30Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2[more]
Q4U4714.6e-1026.20Calumenin OS=Mesocricetus auratus OX=10036 GN=CALU PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CJ229.1e-19599.70calumenin-like OS=Momordica charantia OX=3673 GN=LOC111011909 PE=4 SV=1[more]
A0A6J1FMS71.7e-17789.94calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1[more]
A0A5D3CVB05.5e-17689.22Calumenin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G004... [more]
A0A1S3CNB95.5e-17689.22calumenin-B-like OS=Cucumis melo OX=3656 GN=LOC103502856 PE=4 SV=1[more]
A0A6J1JYP43.0e-17488.17calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111489489 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08580.11.1e-13666.67Calcium-binding EF hand family protein [more]
AT4G27790.12.2e-3629.46Calcium-binding EF hand family protein [more]
AT3G59440.11.2e-0534.38Calcium-binding EF-hand family protein [more]
AT2G43290.12.9e-0430.85Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 158..188
e-value: 140.0
score: 0.6
coord: 239..267
e-value: 0.41
score: 19.7
coord: 288..316
e-value: 2.0
score: 15.4
coord: 200..228
e-value: 53.0
score: 3.8
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 241..266
e-value: 0.0046
score: 16.7
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 205..224
e-value: 0.022
score: 14.2
coord: 292..309
e-value: 0.0052
score: 16.2
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 240..270
score: 8.822743
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 284..319
score: 10.050141
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 205..231
score: 9.854874
NoneNo IPR availableGENE3D1.10.238.10coord: 203..262
e-value: 1.2E-9
score: 40.1
NoneNo IPR availableGENE3D1.10.238.10coord: 265..330
e-value: 2.4E-6
score: 29.5
NoneNo IPR availableGENE3D1.10.238.10coord: 53..190
e-value: 1.0E-5
score: 27.6
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 1..333
NoneNo IPR availablePANTHERPTHR10827:SF83BNAC09G47400D PROTEINcoord: 1..333
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 209..221
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 248..260
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 297..309
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 167..179
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 121..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018678.1MS018678.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding