MS018619 (gene) Bitter gourd (TR) v1

Overview


Name	MS018619
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Eukaryotic translation initiation factor 3 subunit J
Location	scaffold313: 492084 .. 494623 (+)
RNA-Seq Expression	MS018619
Synteny	MS018619

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTCCAGTAGTTAAACCTCCTCCTGAAGCTGTCCCAAAGAAACCTGCCGCCAAAACTACTGAAAAGAAAGGGAAAATTGTTGAGGTAGCACCGCAACAGCCTCTAGATCCTTTAGCCGAGAAACTTCGCCAACAAAGGTAACATTAATTTTCTTTGGTCTGTAGGAACTTTTAGCTTAACTTTTATCTACTTTATGCATCTTTGGAAGGAAAATAATTGAGGCAGGGTTGAACTTGAATTTTTATGAATTGTTTTATTGCGATGGGAGAATCTTTAGAAGCACATCAGGTAACTTCTCTATTCTCCTTAGTCTGGGTGCGCAAAGATGGGCCCCTCTCCCTTTTGCTTTGAAAATGGCTGGATGGAGTATCCCCATTTTTTGAAGCTTGTTGAGAACTGGTGGAAGGAGTTGAATAGTAATGGTTGTCAAAGTTTTGCTTCTGTGCAGAACCTGAAAGGCCTAAAATTGAATATGATAAGATAGCAGTGGTGGTGGGCATAAATAGCAACAACAACGAGGTAGAATAAATAGCAGATAGCCTCGGGTGTAAAGCTGAAAGCTAAGAATCTTCCCATTTCTTATTTAGGCTTTCCTCTTGGAGGAAACCAAAGATCGTGTGGTTTGTGGTGGCAATCAATTGACAAGCTGAAAACAAAGTTAGATAAATGGAGGGGTCTCCTCCTATCAAAGGAATAGAAGGCTGATGTTGGCCCAATCAGCTCTTAACAGCCTGTCAATCTATTTGTTCTCTTTGATAAAAGTCTGCAGAAGGTTCTTAATTCTAGGAAGAAGATAATGAGATATTTTATTTGGAGTGGTGGTAGTTATAAACCTGGTGAATTGGAACCATACCTCTTTGCTCATAAAATATGGAGGTTTAGTTGGGATCCCTTAGACAAGGCTCTCCCAATGAAATGACTTTGGAGGTTTACTCAAGTGAAAGTTGCCCTCTGGAGAAGAGTTATCATATCCGTTTCATTGTTTGATATTAACTGGTGAAAGTCCAACACTCCCCAAGGCAAGGATGGAGGTAGACCTTGGGAAATAGCGCAGAAAAGAGCTAGCTGCATCTCTGATTTTACTTTATTCATGGTTAGGAATAGACCGAAGATTCATTTTTGGGAGGATGCTTGGGTGGATTCCTCTCCCATCTCCTCTAAATTCCTTAACATTTACTCTTCTTCTTTGAAACCTCTTGCTGGAGAATGGAACAAGGCTTGTTCAATAGAGAGCTTGATAATTGGGTTGCCCTAATTGACATTCTTCGGAGGGTTCAGCTGCAAGATGGTGAAGATGGGATTAAGTGGAAGTTGGAAGCAGGTGGAAGCTTCTCTGCCAAATCAGCCTTTTCAGTTTTGACTGAACTTGTGGGAGTTCTAAGGTGTAATTGGGTTAATGTTATATGGGAATACTGCCTCAATAAGAGGGTAGAATTATTTTTGTGGTCCGTTATGTACATAAACATCAATATGCAGCAGAGAAGCTCCAGATGAATTGTTTTTGTGTGTCTTATTATGATTTCTATGGAAGATGTTTGTAGAGTTTCCTTTTAGGAGGAGGATGCTTAACCTTGAAATAGAATCCAAAGTTTCCCCTTCTGATTTTGTAGTGATTTACAAAAATGCCTTGTTTACGTTGTCCCTTTTGGCACCTGCACTTACCTAGTATTTATTTGTGCATTCATTTGATTTCTGCTTCTTTCCTTATTTTGCATGTTTTTTTCTCTCCCTGAATTGTTTAAGCTGAAGATCTCGAAGATTTTTTCTTTCTTTTTTTGGCTTGTAGCTCATGTGAGAATGAACACGTTAGAATGATTCGAAGAGCTTCTACTTGTGTCTTTGGCATGAACTCAGTGGTATGCTTTGTAAAAGATCTTAATCACTTTCTGGGGCTTTTTTTGTCTGTTTCCAAATTGTGGCAGGTTAGTGGAAGAAGCAGATTATAAGTCCACTACTGAATTGTTTAAAAAGAGAGGTGATGAGAAGACCCTCGAAAATTTTATTCCAAAATCTGAGAGTGACTTCTTGGAATATGCAGAGCTTGTCTCTCATAAACTTCTCCCCTATGAGGTACACAATTTACCTTCGTTATCTGTTTTGTTCTATGTGTGAGCAAAGCTTATTCTCATGAGCTTCCCCCAAACTCTTTGTTGCAGAAAAGTTTCCATTATATTAATCTGCTGAAGGACGTTATTAGACTGTCGATGGCTTCACTAAAAGCAGCGGATGCAAAAGACGTTGCCTCTTCTGTTACTGCTCTTGCAAATGAAAAACTAAAGGCAGAGAAGGAAGCGAATGCTGGTAAAAAGAAGACAGGTAAGCATTTGTGTTTATAATCCCCTCTGTTGTCTTCTATTCCGAATTATTTCAATGTGTTACAGACCAGATTGGTCTGAGGTTCTTGTTTGTGAACCGTTTTGTTGTTTCTTGTTTGTGGGGAAACCACTGCAGCTGTGAAAAAGAAGCAGCTTCATGTCGAAAAGGCAGATGATGACCTGGTGGTCAATTCGTATGATGATATCGATGACTACGACTTCATG

mRNA sequence

GCTCCAGTAGTTAAACCTCCTCCTGAAGCTGTCCCAAAGAAACCTGCCGCCAAAACTACTGAAAAGAAAGGGAAAATTGTTGAGGTAGCACCGCAACAGCCTCTAGATCCTTTAGCCGAGAAACTTCGCCAACAAAGGTTAGTGGAAGAAGCAGATTATAAGTCCACTACTGAATTGTTTAAAAAGAGAGGTGATGAGAAGACCCTCGAAAATTTTATTCCAAAATCTGAGAGTGACTTCTTGGAATATGCAGAGCTTGTCTCTCATAAACTTCTCCCCTATGAGAAAAGTTTCCATTATATTAATCTGCTGAAGGACGTTATTAGACTGTCGATGGCTTCACTAAAAGCAGCGGATGCAAAAGACGTTGCCTCTTCTGTTACTGCTCTTGCAAATGAAAAACTAAAGGCAGAGAAGGAAGCGAATGCTGGTAAAAAGAAGACAGCTGTGAAAAAGAAGCAGCTTCATGTCGAAAAGGCAGATGATGACCTGGTGGTCAATTCGTATGATGATATCGATGACTACGACTTCATG

Coding sequence (CDS)

GCTCCAGTAGTTAAACCTCCTCCTGAAGCTGTCCCAAAGAAACCTGCCGCCAAAACTACTGAAAAGAAAGGGAAAATTGTTGAGGTAGCACCGCAACAGCCTCTAGATCCTTTAGCCGAGAAACTTCGCCAACAAAGGTTAGTGGAAGAAGCAGATTATAAGTCCACTACTGAATTGTTTAAAAAGAGAGGTGATGAGAAGACCCTCGAAAATTTTATTCCAAAATCTGAGAGTGACTTCTTGGAATATGCAGAGCTTGTCTCTCATAAACTTCTCCCCTATGAGAAAAGTTTCCATTATATTAATCTGCTGAAGGACGTTATTAGACTGTCGATGGCTTCACTAAAAGCAGCGGATGCAAAAGACGTTGCCTCTTCTGTTACTGCTCTTGCAAATGAAAAACTAAAGGCAGAGAAGGAAGCGAATGCTGGTAAAAAGAAGACAGCTGTGAAAAAGAAGCAGCTTCATGTCGAAAAGGCAGATGATGACCTGGTGGTCAATTCGTATGATGATATCGATGACTACGACTTCATG

Protein sequence

APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELFKKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADAKDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM

Homology

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BLAST of MS018619 vs. NCBI nr
Match: XP_022141435.1 (eukaryotic translation initiation factor 3 subunit J-like [Momordica charantia] >XP_022141436.1 eukaryotic translation initiation factor 3 subunit J-like [Momordica charantia] >XP_022141437.1 eukaryotic translation initiation factor 3 subunit J-like [Momordica charantia])

HSP 1 Score: 325.5 bits (833), Expect = 2.9e-85
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 60
           APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF
Sbjct: 46  APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 105

Query: 61  KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 120
           KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA
Sbjct: 106 KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 165

Query: 121 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM
Sbjct: 166 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 223

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BLAST of MS018619 vs. NCBI nr
Match: XP_038876193.1 (eukaryotic translation initiation factor 3 subunit J-like [Benincasa hispida])

HSP 1 Score: 298.5 bits (763), Expect = 3.8e-77
Identity = 161/178 (90.45%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 60
           APVVKPPPEA PKKPAAK TEKKGK+  V P+QPLDPLAEKLRQQRLVEEADYKSTTELF
Sbjct: 44  APVVKPPPEAAPKKPAAKGTEKKGKV--VVPEQPLDPLAEKLRQQRLVEEADYKSTTELF 103

Query: 61  KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 120
           KK+GDEKTL+NFIPKSESDFLEYAEL+SHKL PYEKSFHYINLLKD IRLSM SLKAADA
Sbjct: 104 KKKGDEKTLDNFIPKSESDFLEYAELISHKLRPYEKSFHYINLLKDAIRLSMVSLKAADA 163

Query: 121 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           KDVASSVTALANEKLKAEKEANAGKKKT+VKKKQLHVEKADDDL+VN+YDD+DDYDFM
Sbjct: 164 KDVASSVTALANEKLKAEKEANAGKKKTSVKKKQLHVEKADDDLIVNAYDDVDDYDFM 219

[Close]

BLAST of MS018619 vs. NCBI nr
Match: XP_004140297.1 (eukaryotic translation initiation factor 3 subunit J [Cucumis sativus] >KGN51421.2 hypothetical protein Csa_008376 [Cucumis sativus])

HSP 1 Score: 294.3 bits (752), Expect = 7.2e-76
Identity = 157/178 (88.20%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 60
           APVVK PPEA PKKPAAK TEKKGKIV+V P++PLDPLAEKLRQQRLVEEADYKSTTELF
Sbjct: 44  APVVK-PPEAAPKKPAAKATEKKGKIVDVEPERPLDPLAEKLRQQRLVEEADYKSTTELF 103

Query: 61  KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 120
           KK+GDEKTL+NFIPKSESDF+EYAEL+SHKL PYEKSFHYINLLKD IRLSM SLKAADA
Sbjct: 104 KKKGDEKTLDNFIPKSESDFVEYAELISHKLRPYEKSFHYINLLKDAIRLSMVSLKAADA 163

Query: 121 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           KDVASS+TALANEKLKAEK+AN GKKKT+VKKKQLHVEKADDDL+VN+YDD+DDYDFM
Sbjct: 164 KDVASSITALANEKLKAEKDANTGKKKTSVKKKQLHVEKADDDLIVNTYDDVDDYDFM 220

[Close]

BLAST of MS018619 vs. NCBI nr
Match: XP_023551414.1 (eukaryotic translation initiation factor 3 subunit J-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291.6 bits (745), Expect = 4.6e-75
Identity = 157/177 (88.70%), Postives = 166/177 (93.79%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELFK 61
           PVVKPPPE  PKKPAAKTTEKK K V V P+QPLDPLAEKLRQQRLVEEADYKSTTELF 
Sbjct: 45  PVVKPPPETAPKKPAAKTTEKKAKTVVVEPKQPLDPLAEKLRQQRLVEEADYKSTTELFG 104

Query: 62  KRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADAK 121
           KRGDEKTL+NFIPKSESDFLEYAEL+SHKL PYEKSFHYINLLKD +RLSM+SLKAADAK
Sbjct: 105 KRGDEKTLDNFIPKSESDFLEYAELISHKLRPYEKSFHYINLLKDAMRLSMSSLKAADAK 164

Query: 122 DVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           +VASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDD++ N+YD IDDYDFM
Sbjct: 165 EVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDMIANTYDAIDDYDFM 221

[Close]

BLAST of MS018619 vs. NCBI nr
Match: XP_022993373.1 (eukaryotic translation initiation factor 3 subunit J-like [Cucurbita maxima] >XP_022993374.1 eukaryotic translation initiation factor 3 subunit J-like [Cucurbita maxima] >KAG6579039.1 Eukaryotic translation initiation factor 3 subunit J, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016562.1 Eukaryotic translation initiation factor 3 subunit J, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 291.2 bits (744), Expect = 6.1e-75
Identity = 157/177 (88.70%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELFK 61
           PVVKPPPE  PKKPAAKTTEKK K V V P+QPLDPLAEKLRQQRLVEEADYKSTTELF 
Sbjct: 45  PVVKPPPETAPKKPAAKTTEKKAKTVVVEPKQPLDPLAEKLRQQRLVEEADYKSTTELFG 104

Query: 62  KRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADAK 121
           KRGDEKTL+NFIPKSESDFLEYAEL+SHKL PYEKSFHYINLLKD +RLSM SLKAADAK
Sbjct: 105 KRGDEKTLDNFIPKSESDFLEYAELISHKLRPYEKSFHYINLLKDAMRLSMTSLKAADAK 164

Query: 122 DVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           +VASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDD++ N+YD IDDYDFM
Sbjct: 165 EVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDMIANTYDAIDDYDFM 221

[Close]

BLAST of MS018619 vs. ExPASy Swiss-Prot
Match: Q54KI0 (Eukaryotic translation initiation factor 3 subunit J OS=Dictyostelium discoideum OX=44689 GN=eif3J PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.6e-15
Identity = 63/186 (33.87%), Postives = 101/186 (54.30%), Query Frame = 0


Query: 4   VKPPPEAVPKKPAAK-----TTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTE 63
           +K PP+ V K+   K       E K K ++  PQ   D   E+ R++ L E +D +++  
Sbjct: 40  IKEPPKPVVKQLTKKQALQRAIELKEKELK-EPQGDFDIYLEEKRKKELQEASDLENSKN 99

Query: 64  LF-----KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMA 123
           LF     K+    + +  FIPK+E DF +Y+++VS  LL Y+ S HY    K +++  + 
Sbjct: 100 LFSGLSVKESSSGEPISTFIPKTEKDFEQYSQIVSDYLLKYDSSIHYATFFKSLMKKVLV 159

Query: 124 SLKAADAKDVASSVTALANEKLKAEKEANAGKK-KTAVKKKQLHVEKADDDLVVNSYDDI 179
           ++ ++D  D++ ++T L NEKLK EK    G K KT V  K++H+        + +YDD 
Sbjct: 160 NVSSSDMNDISKTLTVLINEKLKNEKSGKGGNKTKTKVAPKKIHMGNE-----LQNYDDF 219

[Close]

BLAST of MS018619 vs. ExPASy Swiss-Prot
Match: B4KTH6 (Eukaryotic translation initiation factor 3 subunit J OS=Drosophila mojavensis OX=7230 GN=eIF3j PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 67/184 (36.41%), Postives = 97/184 (52.72%), Query Frame = 0


Query: 1   APVVKPPPEAVPKK----PAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKST 60
           APV   P +A+  K       K  E++ ++ E+ P+   + LAEKLR Q++ EE+D KS 
Sbjct: 56  APVKTKPNKALKAKLEEQERLKEEEEQKRLAEMTPE---EKLAEKLRLQKIQEESDLKSA 115

Query: 61  TELFKKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLK 120
            E F        L+ F P+S+ +F E+   +S K+  Y +S H+   ++D++R    +L 
Sbjct: 116 LETFGVTSIGGGLDAFNPESKEEFKEFGATLSWKVAQYRESIHFPQFIEDLVRSLCCNLS 175

Query: 121 AADAKDVASSVTALANEKLKAEKEANAGKKKTAVK---KKQLHVEKAD-DDLVVNSYDDI 177
           AAD K V  SV +L +EKLK EK   A  KK+A+K   K  L  E  D D       D  
Sbjct: 176 AADIKKVKMSVESLHSEKLKMEK---AAAKKSAMKGKGKVSLRTENDDIDGYQKYGNDFT 233

[Close]

BLAST of MS018619 vs. ExPASy Swiss-Prot
Match: B4J4E7 (Eukaryotic translation initiation factor 3 subunit J OS=Drosophila grimshawi OX=7222 GN=eIF3j PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 58/160 (36.25%), Postives = 85/160 (53.12%), Query Frame = 0


Query: 21  EKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELFKKRGDEKTLENFIPKSESDF 80
           EK+ +   +A     + LAEKLR Q++ EE+D KS  E F     +  L++F P+S  +F
Sbjct: 77  EKEEEAKRLANMSTEEKLAEKLRLQKIQEESDLKSALETFGLTSIDGGLDSFNPQSNEEF 136

Query: 81  LEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADAKDVASSVTALANEKLKAEKE 140
            E+   +S K+  Y++S H+   ++D+++    +L  AD K V  SV  L +EKLK EK 
Sbjct: 137 KEFGATLSWKIAQYKESPHFPQFIEDLVQALCVNLSTADLKKVKMSVEVLHSEKLKMEKV 196

Query: 141 ANAGKKKTAVK---KKQLHVEKADDDLVVNSYDDI-DDYD 177
             + K K A K   K  L  E  D D+     DD  DDYD
Sbjct: 197 --SVKNKGAAKNRGKATLRTENDDIDVYQKYGDDFTDDYD 234

[Close]

BLAST of MS018619 vs. ExPASy Swiss-Prot
Match: Q0VCU8 (Eukaryotic translation initiation factor 3 subunit J OS=Bos taurus OX=9913 GN=EIF3J PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.9e-10
Identity = 56/166 (33.73%), Postives = 80/166 (48.19%), Query Frame = 0


Query: 9   EAVPKKPAAKTTEKK---GKIVEVAPQQ---------------------PLDPLAEKLRQ 68
           E    KP  K +EKK    KI E   QQ                     P + LA+KLR 
Sbjct: 59  EEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRL 118

Query: 69  QRLVEEADYKSTTELFKKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLL 128
           ++L EE+D +   E F        ++   P S  DF E+ +L+  K+  YEKS +Y N L
Sbjct: 119 KKLQEESDLELAKETFGVNNTVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYANFL 178

Query: 129 KDVIRLSMASLKAADAKDVASSVTALANEKLKAEKEANAGKKKTAV 151
           + ++R    SL+  D K + +S+T L +EK K EK++ A KKK  V
Sbjct: 179 EALVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGV 224

[Close]

BLAST of MS018619 vs. ExPASy Swiss-Prot
Match: Q7SXU0 (Eukaryotic translation initiation factor 3 subunit J-A OS=Danio rerio OX=7955 GN=eif3ja PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.2e-10
Identity = 56/146 (38.36%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 31  PQQPLDPLAEKLRQQRLVEEADYKSTTELFKKRGDEKT-LENFIPKSESDFLEYAELVSH 90
           P  P D LAEKLR ++L E++D +   E F    +  T ++   P S+ DF E+  L+  
Sbjct: 97  PLTPEDELAEKLRVKQLQEDSDLELAKEAFGVVSNNVTGIDAVSPSSKDDFTEFERLLKE 156

Query: 91  KLLPYEKSFHYINLLKDVIRLSMASLKAADAKDVASSVTALANEKLKAEKEANAGKKKTA 150
           K+ PYEKS HY   L+ + R    SL+  D K + +S+T L +EK + EK AN GKKK  
Sbjct: 157 KISPYEKSIHYSGFLETLFRDLCLSLEVEDLKKINNSLTVLLSEKQRQEK-ANKGKKK-- 216

Query: 151 VKKKQLHVEKADDDLVVNSYDDIDDY 176
            KK  L        L     DD+ DY
Sbjct: 217 -KKGVL----PGGGLKAKMKDDLADY 234

[Close]

BLAST of MS018619 vs. ExPASy TrEMBL
Match: A0A6J1CJU9 (Eukaryotic translation initiation factor 3 subunit J OS=Momordica charantia OX=3673 GN=LOC111011836 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.4e-85
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 60
           APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF
Sbjct: 46  APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 105

Query: 61  KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 120
           KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA
Sbjct: 106 KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 165

Query: 121 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM
Sbjct: 166 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 223

[Close]

BLAST of MS018619 vs. ExPASy TrEMBL
Match: A0A0A0KTP1 (Eukaryotic translation initiation factor 3 subunit J OS=Cucumis sativus OX=3659 GN=Csa_5G426430 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.5e-76
Identity = 157/178 (88.20%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 60
           APVVK PPEA PKKPAAK TEKKGKIV+V P++PLDPLAEKLRQQRLVEEADYKSTTELF
Sbjct: 43  APVVK-PPEAAPKKPAAKATEKKGKIVDVEPERPLDPLAEKLRQQRLVEEADYKSTTELF 102

Query: 61  KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 120
           KK+GDEKTL+NFIPKSESDF+EYAEL+SHKL PYEKSFHYINLLKD IRLSM SLKAADA
Sbjct: 103 KKKGDEKTLDNFIPKSESDFVEYAELISHKLRPYEKSFHYINLLKDAIRLSMVSLKAADA 162

Query: 121 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           KDVASS+TALANEKLKAEK+AN GKKKT+VKKKQLHVEKADDDL+VN+YDD+DDYDFM
Sbjct: 163 KDVASSITALANEKLKAEKDANTGKKKTSVKKKQLHVEKADDDLIVNTYDDVDDYDFM 219

[Close]

BLAST of MS018619 vs. ExPASy TrEMBL
Match: A0A6J1K212 (Eukaryotic translation initiation factor 3 subunit J OS=Cucurbita maxima OX=3661 GN=LOC111489417 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.9e-75
Identity = 157/177 (88.70%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELFK 61
           PVVKPPPE  PKKPAAKTTEKK K V V P+QPLDPLAEKLRQQRLVEEADYKSTTELF 
Sbjct: 45  PVVKPPPETAPKKPAAKTTEKKAKTVVVEPKQPLDPLAEKLRQQRLVEEADYKSTTELFG 104

Query: 62  KRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADAK 121
           KRGDEKTL+NFIPKSESDFLEYAEL+SHKL PYEKSFHYINLLKD +RLSM SLKAADAK
Sbjct: 105 KRGDEKTLDNFIPKSESDFLEYAELISHKLRPYEKSFHYINLLKDAMRLSMTSLKAADAK 164

Query: 122 DVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           +VASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDD++ N+YD IDDYDFM
Sbjct: 165 EVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDMIANTYDAIDDYDFM 221

[Close]

BLAST of MS018619 vs. ExPASy TrEMBL
Match: A0A6J1FM17 (Eukaryotic translation initiation factor 3 subunit J OS=Cucurbita moschata OX=3662 GN=LOC111445195 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 6.5e-75
Identity = 157/177 (88.70%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELFK 61
           PVVKPPPE  PKKPAAKTTEKK K V V P+QPLDPLAEKLRQQRLVEEADYKSTTELF 
Sbjct: 45  PVVKPPPETAPKKPAAKTTEKKVKTVVVEPKQPLDPLAEKLRQQRLVEEADYKSTTELFG 104

Query: 62  KRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADAK 121
           KRGDEKTL+NFIPKSESDFLEYAEL+SHKL PYEKSFHYINLLKD +RLSM SLKAADAK
Sbjct: 105 KRGDEKTLDNFIPKSESDFLEYAELISHKLRPYEKSFHYINLLKDAMRLSMTSLKAADAK 164

Query: 122 DVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           +VASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDD++ N+YD IDDYDFM
Sbjct: 165 EVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDMIANTYDAIDDYDFM 221

[Close]

BLAST of MS018619 vs. ExPASy TrEMBL
Match: A0A1S3CIM9 (Eukaryotic translation initiation factor 3 subunit J OS=Cucumis melo OX=3656 GN=LOC103501389 PE=3 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.2e-73
Identity = 156/178 (87.64%), Postives = 168/178 (94.38%), Query Frame = 0


Query: 1   APVVKPPPEAVPKKPAAKTTEKKGKIVEVAPQQPLDPLAEKLRQQRLVEEADYKSTTELF 60
           APVVK P EA PKKPA+K TEKKGKIVEV P++PLDPLAEKLRQQRLVEEADYKSTTELF
Sbjct: 44  APVVK-PTEAAPKKPASKATEKKGKIVEVEPERPLDPLAEKLRQQRLVEEADYKSTTELF 103

Query: 61  KKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKAADA 120
           +K+GDEKTL+NFIPKSESDFLEYAEL+SHKL PYEKSFHYINLLKD IRLSM SLKAADA
Sbjct: 104 RKKGDEKTLDNFIPKSESDFLEYAELISHKLRPYEKSFHYINLLKDAIRLSMVSLKAADA 163

Query: 121 KDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDFM 179
           KDVASS+TALANEKLKAEKEAN GKKK +VKKKQLHVEKADDDL+VN+YDD+DDYDFM
Sbjct: 164 KDVASSITALANEKLKAEKEANTGKKK-SVKKKQLHVEKADDDLIVNTYDDVDDYDFM 219

[Close]

BLAST of MS018619 vs. TAIR 10
Match: AT1G66070.1 (Translation initiation factor eIF3 subunit )

HSP 1 Score: 216.9 bits (551), Expect = 1.4e-56
Identity = 123/181 (67.96%), Postives = 148/181 (81.77%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVEV---AP-QQPLDPLAEKLRQQRLVEEADYKSTT 61
           PV+ P PE  PKK A KT EKKGK VEV   AP ++PLDP+AEKLRQQRLVEEADY++T 
Sbjct: 47  PVINPAPEKAPKKAAPKTVEKKGKAVEVPKEAPKEKPLDPIAEKLRQQRLVEEADYRATA 106

Query: 62  ELFKKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKA 121
           ELF  + D+K L+ FIPKSESDFLEYAE++SH++ PYEKS+HYI LLK ++RLS+ ++KA
Sbjct: 107 ELFGVKDDDKNLDMFIPKSESDFLEYAEMISHRIKPYEKSYHYIALLKTIMRLSLTNMKA 166

Query: 122 ADAKDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDF 179
           AD KDVASS+T +ANEKLKAEKEA AGKKK   KKKQL V+KA+DDLV   YD +DD+DF
Sbjct: 167 ADVKDVASSITTIANEKLKAEKEAAAGKKKGG-KKKQLIVDKANDDLVAGPYDAMDDFDF 226

[Close]

BLAST of MS018619 vs. TAIR 10
Match: AT1G66070.2 (Translation initiation factor eIF3 subunit )

HSP 1 Score: 216.9 bits (551), Expect = 1.4e-56
Identity = 123/181 (67.96%), Postives = 148/181 (81.77%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVEV---AP-QQPLDPLAEKLRQQRLVEEADYKSTT 61
           PV+ P PE  PKK A KT EKKGK VEV   AP ++PLDP+AEKLRQQRLVEEADY++T 
Sbjct: 47  PVINPAPEKAPKKAAPKTVEKKGKAVEVPKEAPKEKPLDPIAEKLRQQRLVEEADYRATA 106

Query: 62  ELFKKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKA 121
           ELF  + D+K L+ FIPKSESDFLEYAE++SH++ PYEKS+HYI LLK ++RLS+ ++KA
Sbjct: 107 ELFGVKDDDKNLDMFIPKSESDFLEYAEMISHRIKPYEKSYHYIALLKTIMRLSLTNMKA 166

Query: 122 ADAKDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDF 179
           AD KDVASS+T +ANEKLKAEKEA AGKKK   KKKQL V+KA+DDLV   YD +DD+DF
Sbjct: 167 ADVKDVASSITTIANEKLKAEKEAAAGKKKGG-KKKQLIVDKANDDLVAGPYDAMDDFDF 226

[Close]

BLAST of MS018619 vs. TAIR 10
Match: AT5G37475.1 (Translation initiation factor eIF3 subunit )

HSP 1 Score: 211.1 bits (536), Expect = 7.4e-55
Identity = 121/181 (66.85%), Postives = 146/181 (80.66%), Query Frame = 0


Query: 2   PVVKPPPEAVPKKPAAKTTEKKGKIVE----VAPQQPLDPLAEKLRQQRLVEEADYKSTT 61
           P+VKP  E  PKKPA K  EKK K VE     + ++PLDP+AEKLR QRLVEEADY+ST 
Sbjct: 46  PIVKPASEKAPKKPAVKAVEKKVKTVEAPKGTSREEPLDPIAEKLRMQRLVEEADYQSTA 105

Query: 62  ELFKKRGDEKTLENFIPKSESDFLEYAELVSHKLLPYEKSFHYINLLKDVIRLSMASLKA 121
           ELF  + +EK+++  IPKSESDFL+YAEL+S +L+P+EKSFHYI LLK V+RLS+A++KA
Sbjct: 106 ELFGVKTEEKSVDMLIPKSESDFLDYAELISQRLVPFEKSFHYIGLLKAVMRLSVANMKA 165

Query: 122 ADAKDVASSVTALANEKLKAEKEANAGKKKTAVKKKQLHVEKADDDLVVNSYDDIDDYDF 179
           AD KDVASS+TA+ANEKLKAEKEA AGKKK+  KKKQLHV+K DDDLV   YD +DD DF
Sbjct: 166 ADVKDVASSITAIANEKLKAEKEAAAGKKKSG-KKKQLHVDKPDDDLVSGPYDAMDDDDF 225

The following BLAST results are available for this feature:

BLAST of MS018619 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141435.1	2.9e-85	100.00	eukaryotic translation initiation factor 3 subunit J-like [Momordica charantia] ...	[more]
XP_038876193.1	3.8e-77	90.45	eukaryotic translation initiation factor 3 subunit J-like [Benincasa hispida]	[more]
XP_004140297.1	7.2e-76	88.20	eukaryotic translation initiation factor 3 subunit J [Cucumis sativus] >KGN51421...	[more]
XP_023551414.1	4.6e-75	88.70	eukaryotic translation initiation factor 3 subunit J-like [Cucurbita pepo subsp....	[more]
XP_022993373.1	6.1e-75	88.70	eukaryotic translation initiation factor 3 subunit J-like [Cucurbita maxima] >XP...	[more]

BLAST of MS018619 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54KI0	5.6e-15	33.87	Eukaryotic translation initiation factor 3 subunit J OS=Dictyostelium discoideum...	[more]
B4KTH6	2.2e-11	36.41	Eukaryotic translation initiation factor 3 subunit J OS=Drosophila mojavensis OX...	[more]
B4J4E7	4.9e-11	36.25	Eukaryotic translation initiation factor 3 subunit J OS=Drosophila grimshawi OX=...	[more]
Q0VCU8	1.9e-10	33.73	Eukaryotic translation initiation factor 3 subunit J OS=Bos taurus OX=9913 GN=EI...	[more]
Q7SXU0	3.2e-10	38.36	Eukaryotic translation initiation factor 3 subunit J-A OS=Danio rerio OX=7955 GN...	[more]

BLAST of MS018619 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJU9	1.4e-85	100.00	Eukaryotic translation initiation factor 3 subunit J OS=Momordica charantia OX=3...	[more]
A0A0A0KTP1	3.5e-76	88.20	Eukaryotic translation initiation factor 3 subunit J OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1K212	2.9e-75	88.70	Eukaryotic translation initiation factor 3 subunit J OS=Cucurbita maxima OX=3661...	[more]
A0A6J1FM17	6.5e-75	88.70	Eukaryotic translation initiation factor 3 subunit J OS=Cucurbita moschata OX=36...	[more]
A0A1S3CIM9	1.2e-73	87.64	Eukaryotic translation initiation factor 3 subunit J OS=Cucumis melo OX=3656 GN=...	[more]

BLAST of MS018619 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66070.1	1.4e-56	67.96	Translation initiation factor eIF3 subunit	[more]
AT1G66070.2	1.4e-56	67.96	Translation initiation factor eIF3 subunit	[more]
AT5G37475.1	7.4e-55	66.85	Translation initiation factor eIF3 subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013906	Eukaryotic translation initiation factor 3 subunit J	PFAM	PF08597	eIF3_subunit	coord: 18..178 e-value: 2.8E-39 score: 135.5
IPR013906	Eukaryotic translation initiation factor 3 subunit J	PANTHER	PTHR21681	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J	coord: 4..178
IPR023194	Eukaryotic translation initiation factor 3-like domain superfamily	GENE3D	1.10.246.60	Eukaryotic translation initiation factor 3 like domains	coord: 67..144 e-value: 3.9E-24 score: 86.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR21681:SF2	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J	coord: 4..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018619.1	MS018619.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005852	eukaryotic translation initiation factor 3 complex
molecular_function	GO:0003743	translation initiation factor activity