MS018591 (gene) Bitter gourd (TR) v1

Overview
NameMS018591
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptiontRNA modification GTPase MnmE
Locationscaffold313: 150342 .. 154969 (-)
RNA-Seq ExpressionMS018591
SyntenyMS018591
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTGCTTCCAGGATTCCGCCATTTCATCACCCATTTTTACAGAACTTCACCCTCCATGGCTTTTCTCTTAACCCACCTCCATGCCCCTGTTTCCACACCATGCTCTCTCTCCCACATACCCAAATCCTCCAACCCTGTTCTTCCAAAATCTCTTACAAAGTCCCACGCTACTCCAAAACGTCATTCCCCTATCAATTTCTCTACTTCATCATTTCAAATTCACTGGGTTTTGCCATTTCCTCATTCTTGTTTCTTTTTCTGAGTGCAGATAATGCCTTGGTTTTGACTCGAGATGAGCGCCTGGAGAATTCTCGTGCCGAACAAATTGAGAATTCGAGCACAATTGCGGCCATCGTGACGTCCATGGGGGGTTCCCCTGCTGCGGTGGGGATTGTGCGATTATCGGGTCCTCGTGCTGTGACTATAGTTGGGAGTTTATTTCGCCCCGCCGCGAGAAAGAAGACGAAGAATTTGAGTCCGCATTCATGGAGACCAACTAGCCATGTCGTGGAGTACGGTGCGGTTTTGGACCAACAGGGCGATGTAATTGACGAAGTACGGTAACGTTTCCTTTGCCATGGTTTGATGAATTCTTAAGTCTGAGATGAAACTTCCATTGGATTAATTTAAGCCATGGTTGTACTCTTTTGTGAAGGTTTTGGCGGTGCCGATGCTGGCCCCAAGATCTTATACTCGTGAAGATGTGGTTGAGCTTCAGTGTCACGGGAGTGAAGTATGTCTCCGACGTGTGCTAAAGGCTTGTTTAGAAGCTGGAGCCCGGCTTGCAGAACCAGGTTTGTTTACTGAACTTGAATTGCTTTGTCATTAAATTCATAGTCTAAACACCTTATTTAATTGCTTTGTCATTAAATTCATAGTCTAAACACCTTGTTTTATGTATGGTGTTCGAGCCTACTTGTGCACAATTTGACTCTATCTCACCCACGAGACAACTGCCTGCTGTTGGTCTGTACACAATTGTCTTCACATCTGTCTATACCAACTGAAGTATGCACTTGCACGCATGTAAATAATGCAGTGCTAAAGCATGAAAGAAAATTTGGAAAATTGGTTCAAGAAAAAAGTTTATGCTTTATGATCTCACCCTTATCAAATCCACACTTGTGCCAATCGATTACCCGGGAAGACAGAAACTTTTATATTTACATATTTATATTCCCGTTTTTAGGTGAATTTACTCTTCGTGCCTTCTTAAATGGCCGCTTAGACCTTTCCCAAGCAGAAAATGTTGGGAAACTAATTTCAGCCAAGTCTACGGCTGCTGCAGATGCTGCGTTGGCTGGAATTCAGGTCGGGGCTCTTATTTAACTGATAATGTTGTCTGAACTGGTGTGACCATCTATTTTCAGTTACCGGGTTACTGCAACTTAATTTGGAAATCATCAATGACTGTTTCATAACTGAAGTATATAGTTTTTTGATTGACAGGGAGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGCATTGAATTACTCACTGAAATCGAGGCCCGTCTAGACTTTGATGATGAGATGCCACCATTAGACTTGGAACTTATAATGGATAAAGTACATGCCATGTCACAAGATGTAGAGACTGCTCTTGAGACGGCCAATTATGACAAGCTTCTTCAGTCTGGAATACAGGTAAATTTTAAGAGTCGTCTAATAGTCTGTTCCTGTGTAAAGTAGATGTATAGATTTGTTAATTCCTTCAGCAATCTCCTTAAAACTTGCAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCAAGCCTTCTCAATGCATGGAGCAGAGTAAGCTACACGATGTTCTCTTTTTTCCTTCCAACATGATCTCCTGTAGAAAATTTGTTCTATCTGTGAGTCTGACCTGTTATCTGATAATCTGCACGTCACTTTTTGCTTGTTCCTTGTTATTTCCATTGAATAGTCAGATTTCCACTTACTCTTGATATCGTCTAGTGCTTTGTCCCATAAGAGAGAGGGATATTCTTGCTCGACTTTTCAGATTTAGTGAAATGTGAATTGAAATCTGAGATTCAAAATCTTGATAATGTATTACAACTTTCAGGAGTAAAGGTTAAATCTGAAGACCTTCTAACAAAATGCATGATTCTTCACACTAGTAATGGTCTTTCTGAAAATTTTGGTGAAAAATCTGAAGATTCTTCCTGAACTTATATTCAAGGAGAGTGCTCACCCTTCAACTTTTCTAGCCTGCACATGGAAAGGATTGATACAGAAGGAAAATAGGTTTAGGAGAACAAGGAGGATTTGGTAGTAACCCAAATGAGGAAGGGCCGAAGGGGCTCTACTTCAACTTTTCTAGGAACAGGAACATTGTGTTGTGACGCTTACCTCTTCCGTCAAATTCGTCGCAAAAATAGGGAAGATAGTCTGTCCTTTTGTGCAGATTTCTGGGAAGGTGGAGGAGAGCAGGGATTGTTCGTCTAAAACAAGTTGGTCAAGGAGTGGATAATCTGCCAGATGCTATATTGGATATCAAACTTGCCAAGTGCCTTTTGCCAATAATCTGTTCAGTGTGCCTATGTTGATGAGAAAATCTAAGAAGAGAATGCTTATTAGGGAAAAAGATGGAAGCCTGTTGTGCGACCCTTCCCTCCATGGTTAAGTACTAGAACATCTACAGGGACTCCTAGTGTCTAACGTATGTTTTTAGCTTGAACATGACATTTAGGGGCTAGATCTGAAATAAGCCTTGCCGAAGACACATTTTGGAAGAGCAGGACTATGAAATTGAGTTTGGTTGATAGGTTGTTTGTGAAGTCCATCTTGAGCTCTAGAGACATTGCTTTGGCCTTGCTGGATGGATAATTGGGACACATTTTTAGCCACTTCTTAGTGCTTCCCACAAGAATTTCAGAAAGTTCCATTGTAGCTGTTTTGTGACCACATGTTTAAGAAGCAAGGTTAGGGTTCTTTGGCTTGATGCTATTAAGTCTCTTTTATGAGAAAGTGTGGTTAGAGAGAAATAGAGAATCTTAAATGGAGTGTACAGGAAAGAGCTTCTCAGAATGTTCAGAATGTGATTGTTTTCGTGGCCTTGTCTTGGAGTGTTTTTTCTAATTCTTTTGCTACTCATTATCTACTACGCTGGCCCAGAGGAGTTTCTTCCTGTAACACTTTGGCATTTGTGAGGGTGATTTCCCTCCTTTGTAATTTCATTTATGCTATGAAAGTTTTGTGTGTTACTTATGGAAAGAGACATTGTAAACTTGTGTTCATGTTAAAGCATGCCAAAATATTTGTAATCTCATAATGAAGTTTCCTGTTAGATATAGGTGGTTTTCTTTAGCTACTTTTTTCTTGAACTCTTAATCACGTCATTCTATTGTCACATTTATTTTTTAGCGTGCAGAGCGAGAGGGCGATAGTTACAGAAATTGCTGGAACTACCAGGGACGTAATTGAGGCGAATGTCACAGTTTGTGGTATCCCTGTGACTCTTCTGGATACAGCTGGAATTAGGGAAACAGATGACATTGTGGAAAAGATTGGTATGATTTATGAATTTTTAATGTCTTCATTCAGCATTTCAAGGGTTTGGACATTGCATTTAGTGTCATGCCATATAAATATATGATTATTTTCCAAAACAGTGTATGGCAATTGCAAATTACACAGACCACTTGAGTGTAAACTTGCAGTTCATAACTTTTTGATCGTTAACTTACGATGCGCTACAGGGTATTCTCATGATGCTCAATTAGTCGCTTTAAGCCAAGTTGATTTGCATTTAGTTTGTGTAGTTGTAACTTGTCCTGTGGTCTATTTACAGGCGTAGAGAGATCTGAAGCTGCTGCTCTTGGGGCTGATGTCATTATTATGACTATAAGCGCCCTAGACGGATGGACTACAGAAGATACCATACTTCTTGATAGGATACAGTCAAAAAAGGTTAAACAACAAAATTCCACCACCCTTTCCTCCAATGAACTCTGCTTTATTGAGTTAATGGTCAATATCAGAACTCCACCACTTTAAAGAAATTTGGAAGTTGGTGTTTATGTTTTTTTTTTTTTTTGTCCTTTTAAATTCTTGAAAAATACAGAAATCAAATGGATCAGCCACTCCTATACTTCTGGTAATAAACAAGATAGACTGTGTTCCATCTCCAAACATGGATGATCTTGGTATGAACTATGATTCTTTCAATAAACAAGTTTATACATGCGCTGTCACCGGACAAGGAATAGAGAATCTGGAGATGGCAATATCCGAGCTGGTGGGTCTCGACAAAACTCTTGCAAGTGGACGTAGATGGACAGTAAACCAGGTAACCTATTCCACCCTACCCATCTCCAGATAAGAACCCAGTTGCAGACTGCCTAAAACTAAAAATTACAGTATTCCTTGTCTTAATAAATTGATGTATTAATGAGCAATAGAACTTATAGTTTAGTGCTGTTCTGGTTACAATCAGAGACAATGTGAGCAGCTTCTTCGAACCAAGGAGGCGCTTGCAAGACTAAAATCTTCCATTGAAGAGGAGTTGCCTCTTGATTTTTGGACAGTGGATTTGAGAGGTGCTGCTTTAGCTCTTGGGCAAATAAGTGGTGACGATATCTCTGAAGAGGTTTTGTCCAATATT

mRNA sequence

ATGGCTCTGCTTCCAGGATTCCGCCATTTCATCACCCATTTTTACAGAACTTCACCCTCCATGGCTTTTCTCTTAACCCACCTCCATGCCCCTGTTTCCACACCATGCTCTCTCTCCCACATACCCAAATCCTCCAACCCTGTTCTTCCAAAATCTCTTACAAAGTCCCACGCTACTCCAAAACATAATGCCTTGGTTTTGACTCGAGATGAGCGCCTGGAGAATTCTCGTGCCGAACAAATTGAGAATTCGAGCACAATTGCGGCCATCGTGACGTCCATGGGGGGTTCCCCTGCTGCGGTGGGGATTGTGCGATTATCGGGTCCTCGTGCTGTGACTATAGTTGGGAGTTTATTTCGCCCCGCCGCGAGAAAGAAGACGAAGAATTTGAGTCCGCATTCATGGAGACCAACTAGCCATGTCGTGGAGTACGGTGCGGTTTTGGACCAACAGGGCGATGTAATTGACGAAGTTTTGGCGGTGCCGATGCTGGCCCCAAGATCTTATACTCGTGAAGATGTGGTTGAGCTTCAGTGTCACGGGAGTGAAGTATGTCTCCGACGTGTGCTAAAGGCTTGTTTAGAAGCTGGAGCCCGGCTTGCAGAACCAGGTGAATTTACTCTTCGTGCCTTCTTAAATGGCCGCTTAGACCTTTCCCAAGCAGAAAATGTTGGGAAACTAATTTCAGCCAAGTCTACGGCTGCTGCAGATGCTGCGTTGGCTGGAATTCAGGGAGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGCATTGAATTACTCACTGAAATCGAGGCCCGTCTAGACTTTGATGATGAGATGCCACCATTAGACTTGGAACTTATAATGGATAAAGTACATGCCATGTCACAAGATGTAGAGACTGCTCTTGAGACGGCCAATTATGACAAGCTTCTTCAGTCTGGAATACAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCAAGCCTTCTCAATGCATGGAGCAGAAGCGAGAGGGCGATAGTTACAGAAATTGCTGGAACTACCAGGGACGTAATTGAGGCGAATGTCACAGTTTGTGGTATCCCTGTGACTCTTCTGGATACAGCTGGAATTAGGGAAACAGATGACATTGTGGAAAAGATTGGCGTAGAGAGATCTGAAGCTGCTGCTCTTGGGGCTGATGTCATTATTATGACTATAAGCGCCCTAGACGGATGGACTACAGAAGATACCATACTTCTTGATAGGATACAGTCAAAAAAGAAATCAAATGGATCAGCCACTCCTATACTTCTGGTAATAAACAAGATAGACTGTGTTCCATCTCCAAACATGGATGATCTTGGTATGAACTATGATTCTTTCAATAAACAAGTTTATACATGCGCTGTCACCGGACAAGGAATAGAGAATCTGGAGATGGCAATATCCGAGCTGGTGGGTCTCGACAAAACTCTTGCAAGTGGACGTAGATGGACAGTAAACCAGAGACAATGTGAGCAGCTTCTTCGAACCAAGGAGGCGCTTGCAAGACTAAAATCTTCCATTGAAGAGGAGTTGCCTCTTGATTTTTGGACAGTGGATTTGAGAGGTGCTGCTTTAGCTCTTGGGCAAATAAGTGGTGACGATATCTCTGAAGAGGTTTTGTCCAATATT

Coding sequence (CDS)

ATGGCTCTGCTTCCAGGATTCCGCCATTTCATCACCCATTTTTACAGAACTTCACCCTCCATGGCTTTTCTCTTAACCCACCTCCATGCCCCTGTTTCCACACCATGCTCTCTCTCCCACATACCCAAATCCTCCAACCCTGTTCTTCCAAAATCTCTTACAAAGTCCCACGCTACTCCAAAACATAATGCCTTGGTTTTGACTCGAGATGAGCGCCTGGAGAATTCTCGTGCCGAACAAATTGAGAATTCGAGCACAATTGCGGCCATCGTGACGTCCATGGGGGGTTCCCCTGCTGCGGTGGGGATTGTGCGATTATCGGGTCCTCGTGCTGTGACTATAGTTGGGAGTTTATTTCGCCCCGCCGCGAGAAAGAAGACGAAGAATTTGAGTCCGCATTCATGGAGACCAACTAGCCATGTCGTGGAGTACGGTGCGGTTTTGGACCAACAGGGCGATGTAATTGACGAAGTTTTGGCGGTGCCGATGCTGGCCCCAAGATCTTATACTCGTGAAGATGTGGTTGAGCTTCAGTGTCACGGGAGTGAAGTATGTCTCCGACGTGTGCTAAAGGCTTGTTTAGAAGCTGGAGCCCGGCTTGCAGAACCAGGTGAATTTACTCTTCGTGCCTTCTTAAATGGCCGCTTAGACCTTTCCCAAGCAGAAAATGTTGGGAAACTAATTTCAGCCAAGTCTACGGCTGCTGCAGATGCTGCGTTGGCTGGAATTCAGGGAGGCTTCTCTTCCTTAGTCAAATTGCTAAGAACACAATGCATTGAATTACTCACTGAAATCGAGGCCCGTCTAGACTTTGATGATGAGATGCCACCATTAGACTTGGAACTTATAATGGATAAAGTACATGCCATGTCACAAGATGTAGAGACTGCTCTTGAGACGGCCAATTATGACAAGCTTCTTCAGTCTGGAATACAGATAGCAATTGTTGGCCGCCCTAATGTTGGGAAGTCAAGCCTTCTCAATGCATGGAGCAGAAGCGAGAGGGCGATAGTTACAGAAATTGCTGGAACTACCAGGGACGTAATTGAGGCGAATGTCACAGTTTGTGGTATCCCTGTGACTCTTCTGGATACAGCTGGAATTAGGGAAACAGATGACATTGTGGAAAAGATTGGCGTAGAGAGATCTGAAGCTGCTGCTCTTGGGGCTGATGTCATTATTATGACTATAAGCGCCCTAGACGGATGGACTACAGAAGATACCATACTTCTTGATAGGATACAGTCAAAAAAGAAATCAAATGGATCAGCCACTCCTATACTTCTGGTAATAAACAAGATAGACTGTGTTCCATCTCCAAACATGGATGATCTTGGTATGAACTATGATTCTTTCAATAAACAAGTTTATACATGCGCTGTCACCGGACAAGGAATAGAGAATCTGGAGATGGCAATATCCGAGCTGGTGGGTCTCGACAAAACTCTTGCAAGTGGACGTAGATGGACAGTAAACCAGAGACAATGTGAGCAGCTTCTTCGAACCAAGGAGGCGCTTGCAAGACTAAAATCTTCCATTGAAGAGGAGTTGCCTCTTGATTTTTGGACAGTGGATTTGAGAGGTGCTGCTTTAGCTCTTGGGCAAATAAGTGGTGACGATATCTCTGAAGAGGTTTTGTCCAATATT

Protein sequence

MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATPKHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI
Homology
BLAST of MS018591 vs. NCBI nr
Match: XP_022141643.1 (uncharacterized protein LOC111011948 [Momordica charantia])

HSP 1 Score: 1050.4 bits (2715), Expect = 5.3e-303
Identity = 547/549 (99.64%), Postives = 548/549 (99.82%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP
Sbjct: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60

Query: 61  KHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFR 120
           KHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFR
Sbjct: 61  KHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFR 120

Query: 121 PAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCH 180
           PAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCH
Sbjct: 121 PAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCH 180

Query: 181 GSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAAL 240
           GSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA AAL
Sbjct: 181 GSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAAHAAL 240

Query: 241 AGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALET 300
           AGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLEL+MDKVHAMSQDVETALET
Sbjct: 241 AGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELVMDKVHAMSQDVETALET 300

Query: 301 ANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPV 360
           ANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPV
Sbjct: 301 ANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPV 360

Query: 361 TLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKS 420
           TLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKS
Sbjct: 361 TLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKS 420

Query: 421 NGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLD 480
           NGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLD
Sbjct: 421 NGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLD 480

Query: 481 KTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDD 540
           KTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDD
Sbjct: 481 KTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDD 540

Query: 541 ISEEVLSNI 550
           ISEEVLSNI
Sbjct: 541 ISEEVLSNI 549

BLAST of MS018591 vs. NCBI nr
Match: XP_038874821.1 (tRNA modification GTPase MnmE [Benincasa hispida])

HSP 1 Score: 926.8 bits (2394), Expect = 8.8e-266
Identity = 486/553 (87.88%), Postives = 511/553 (92.41%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHFYRT+P MAFLLTH H P+S P SLS IPK SNPVLPKSL K H+T 
Sbjct: 1   MALLPGFRHFITHFYRTTPPMAFLLTHFHTPISRPSSLSSIPKPSNPVLPKSLIKFHSTG 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K N  VL  DERL +S A    EQIENSSTIAA+VTSMGGSPAAVGIVRLSGPRAV IVG
Sbjct: 61  KENTFVLAPDERLADSHAGIEREQIENSSTIAAVVTSMGGSPAAVGIVRLSGPRAVDIVG 120

Query: 121 SLFRPAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVE 180
           SLF PA +KK KNLS H WRPTSHVVEYG VLD+QGDVIDEVL VPMLAPRSYTREDV+E
Sbjct: 121 SLFCPAGKKKGKNLSLHPWRPTSHVVEYGVVLDKQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVET 300
           DAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPL L++IM+KVH MSQ+VE 
Sbjct: 241 DAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLVLDIIMEKVHTMSQEVEA 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVC 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV 
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWT EDTILL+RI S
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTAEDTILLNRILS 420

Query: 421 KKKSNGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISEL 480
           KKKS+GS+TPILLVINKIDC PSPNMD + +N+DSF+KQV+TCAVTGQGIENLEM ISEL
Sbjct: 421 KKKSDGSSTPILLVINKIDCAPSPNMDAISINHDSFSKQVFTCAVTGQGIENLEMTISEL 480

Query: 481 VGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQI 540
           VGL+KTLASGRRWTVNQRQCEQLLRTKEAL RLKSSIE+ELPLDFWTVDLR AALALG+I
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEALTRLKSSIEDELPLDFWTVDLRDAALALGEI 540

Query: 541 SGDDISEEVLSNI 550
            G+DISEEVLSNI
Sbjct: 541 CGEDISEEVLSNI 553

BLAST of MS018591 vs. NCBI nr
Match: XP_022992856.1 (uncharacterized protein LOC111489063 isoform X1 [Cucurbita maxima])

HSP 1 Score: 912.5 bits (2357), Expect = 1.7e-261
Identity = 484/555 (87.21%), Postives = 509/555 (91.71%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHF RT+P MAFL TH H P+S P SL HIP  S+PVLPKSL KSHA+ 
Sbjct: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASG 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K     LTRDERLE+S A    EQIENSSTIAAIVTSMGG PAAVGIVRLSGPRAV IVG
Sbjct: 61  KKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFRPAARKKTKNLS-PHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVV 180
           SLF PAA+KK KN+S  H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+
Sbjct: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVE 300
           ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPL+LE IM+KVHAM+Q+VE
Sbjct: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 CGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQ 420
            GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWT EDTILLD I 
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420

Query: 421 SKKKSNGSATPILLVINKIDCVPS-PNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAIS 480
           +KKKS+GS TPILLVINKIDC PS  NMD + +N DSF+KQV+TCAVTGQGIE+LEMAIS
Sbjct: 421 AKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480

Query: 481 ELVGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALG 540
           EL+GL+ TLASGRRWTVNQRQCEQLLRTKEAL RLKSSIEEELPLDFWTVDLRGA LALG
Sbjct: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540

Query: 541 QISGDDISEEVLSNI 550
           +ISG+DISEEVLSNI
Sbjct: 541 EISGEDISEEVLSNI 555

BLAST of MS018591 vs. NCBI nr
Match: XP_023549904.1 (uncharacterized protein LOC111808259 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 903.3 bits (2333), Expect = 1.0e-258
Identity = 478/555 (86.13%), Postives = 508/555 (91.53%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHF+THF RT+P M FLLTH + P+S P SL HIP  S PVLPK+L K HA+ 
Sbjct: 1   MALLPGFRHFVTHFCRTTPPMPFLLTHFYTPISRPSSLFHIPNPSCPVLPKALIKPHASG 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K     L+RDERLE+S A    EQIENSSTIAAIVTSMGG PAAVGIVRLSGPRAV IVG
Sbjct: 61  KKTTFFLSRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVKIVG 120

Query: 121 SLFRPAARKKTKNLS-PHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVV 180
           SLF PAA+KK KN+S  H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+
Sbjct: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVE 300
           ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPL+LE IM+KVHAM+Q+VE
Sbjct: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 CGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQ 420
            GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWT EDT+LLD I 
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTAEDTVLLDSIL 420

Query: 421 SKKKSNGSATPILLVINKIDCVPS-PNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAIS 480
           +KKKS+GS+TPILLVINKIDC PS  NMD + +N DSF+KQV+TCAVTGQGIE+LEMAIS
Sbjct: 421 AKKKSDGSSTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480

Query: 481 ELVGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALG 540
           EL+GL+ TLASGRRWTVNQRQCEQLLRTKEAL RLKSSIEEELPLDFWTVDLRGA LALG
Sbjct: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540

Query: 541 QISGDDISEEVLSNI 550
           +ISG+DISEEVLSNI
Sbjct: 541 EISGEDISEEVLSNI 555

BLAST of MS018591 vs. NCBI nr
Match: KAG6579011.1 (hypothetical protein SDJN03_23459, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 480/555 (86.49%), Postives = 508/555 (91.53%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHF RT+P M FLLTH + P+S P SL  IP  S PVLPKSL KSHA+ 
Sbjct: 63  MALLPGFRHFITHFCRTTPPMPFLLTHFYNPISRPSSLFRIPNPSCPVLPKSLIKSHASG 122

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           +     LTRDERLE+S A    EQIENSSTIAAIVTSMGG PAAVGIVRLSGPRAV IVG
Sbjct: 123 RETTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 182

Query: 121 SLFRPAARKKTKNLS-PHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVV 180
           SLF PAA+KK KN+S  H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+
Sbjct: 183 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 242

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 243 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 302

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVE 300
           ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPL+LE IM+KVHAM+Q+VE
Sbjct: 303 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 362

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV
Sbjct: 363 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 422

Query: 361 CGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQ 420
            GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWT EDTILLD I 
Sbjct: 423 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 482

Query: 421 SKKKSNGSATPILLVINKIDCVPS-PNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAIS 480
           +KKKS+GS+TPILLVINKIDC PS  NMD + +N DSF+KQV+TCAVTGQGIE+LEMAIS
Sbjct: 483 AKKKSDGSSTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 542

Query: 481 ELVGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALG 540
           EL+GL+ TLA+GRRWTVNQRQCEQLLRTKEAL RLKSSIEEELPLDFWTVDLRGA LALG
Sbjct: 543 ELMGLNNTLANGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 602

Query: 541 QISGDDISEEVLSNI 550
           +ISG+DISEEVLSNI
Sbjct: 603 EISGEDISEEVLSNI 617

BLAST of MS018591 vs. ExPASy Swiss-Prot
Match: Q8KPU2 (tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=mnmE PE=3 SV=3)

HSP 1 Score: 422.2 bits (1084), Expect = 9.2e-117
Identity = 231/468 (49.36%), Postives = 315/468 (67.31%), Query Frame = 0

Query: 83  NSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPTSHVV 142
           +  TIAAI T++     +VGIVRLSG  A  I   +F+ A ++         W   SH +
Sbjct: 5   SGDTIAAIATAIVPQQGSVGIVRLSGAAATEIARQIFQIAGQQ--------PWE--SHRI 64

Query: 143 EYGAVLD-QQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGARLA 202
            YG + D + G ++DE L +PMLAPRSYTREDVVEL CHG  + +++ L+ C+ AGARLA
Sbjct: 65  LYGYIRDPESGRLVDEALLLPMLAPRSYTREDVVELHCHGGLMPVQQTLQLCIRAGARLA 124

Query: 203 EPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIEL 262
           EPGEFTLRAFLNGRLDLSQAE++  LISA+S  AA AAL  +QG     ++ LR +C+++
Sbjct: 125 EPGEFTLRAFLNGRLDLSQAESIADLISAQSPQAAQAALGSLQGKLGHPIRQLRDRCLDI 184

Query: 263 LTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNV 322
           L E+EAR+DF+D++PPLDLE I  ++ A   D++  L TA+  +LL++G++IAIVGRPNV
Sbjct: 185 LAEVEARIDFEDDLPPLDLEAIAAQLTAAGADMQAILSTADRGELLRTGLKIAIVGRPNV 244

Query: 323 GKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKIGVE 382
           GKSSLLNAWSR +RAIVT++ GTTRD++E+ + V GIPV +LDTAGIRET D VE+IGVE
Sbjct: 245 GKSSLLNAWSRCDRAIVTDLPGTTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGVE 304

Query: 383 RSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPSPN 442
           RS  AA  AD++++TI A  GW+ ED  + + +  +        PILLVINK D +    
Sbjct: 305 RSRRAAQSADLVLLTIDASAGWSAEDQTIWEAVSDR--------PILLVINKRDRLSEAE 364

Query: 443 MDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQCEQLLR 502
              + +    F   V+T A   QGIE+LE AI   VG     ++   W +NQRQ   L  
Sbjct: 365 RHAIALPQQEFKAIVWTAAAQQQGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAALTT 424

Query: 503 TKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI 550
            + AL R++ +++ +LPLDFWT+DLR A  ALG I+G++I+E +L  I
Sbjct: 425 AQTALRRVEETLQAQLPLDFWTIDLREAIAALGSITGEEIAESMLDLI 454

BLAST of MS018591 vs. ExPASy Swiss-Prot
Match: Q5N638 (tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=mnmE PE=3 SV=2)

HSP 1 Score: 417.2 bits (1071), Expect = 3.0e-115
Identity = 229/468 (48.93%), Postives = 313/468 (66.88%), Query Frame = 0

Query: 83  NSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPTSHVV 142
           +  TIAAI T++     +VGIVRLSG  A  I   +F+ A ++         W   SH +
Sbjct: 5   SGDTIAAIATAIVPQQGSVGIVRLSGAAATEIARQIFQIAGQQ--------PWE--SHRI 64

Query: 143 EYGAVLD-QQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGARLA 202
            YG + D + G ++DE L +PMLAPRSYTREDVVEL CHG  + +++ L+ C+ AGARLA
Sbjct: 65  LYGYIRDPESGRLVDEALLLPMLAPRSYTREDVVELHCHGGLMPVQQTLQLCIRAGARLA 124

Query: 203 EPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIEL 262
           EPGEFTLRAFLNGRLDLSQAE++  LISA+S  AA AAL  +QG     ++ LR +C+++
Sbjct: 125 EPGEFTLRAFLNGRLDLSQAESIADLISAQSPQAAQAALGSLQGKLGHPIRQLRDRCLDI 184

Query: 263 LTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNV 322
           L E+EAR+DF+D++PPLDLE I  ++ A   D++  L TA+  +LL++G++IAIVGRPNV
Sbjct: 185 LAEVEARIDFEDDLPPLDLEAIAAQLTAAGADMQAILSTADRGELLRTGLKIAIVGRPNV 244

Query: 323 GKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKIGVE 382
           GKSSLLNAWSR +RAIVT++ G TRD++E+ + V GIPV +LDTAGIRET D VE+IGVE
Sbjct: 245 GKSSLLNAWSRCDRAIVTDLPGRTRDLVESQLIVGGIPVQVLDTAGIRETSDQVEQIGVE 304

Query: 383 RSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPSPN 442
           RS  AA  AD++++TI A  GW+ ED  + + +  +        PILLVINK D +    
Sbjct: 305 RSRRAAQSADLVLLTIDASAGWSAEDQTIWEAVSDR--------PILLVINKRDRLSEAE 364

Query: 443 MDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQCEQLLR 502
              + +    F   V+T A   +GIE+LE AI   VG     ++   W +NQRQ   L  
Sbjct: 365 RHAIALPQQEFKAIVWTAAAQQKGIEDLEAAILAAVGTGDLTSANWDWALNQRQVAALTT 424

Query: 503 TKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI 550
            + AL R++ +++ +LPLDFWT+DLR A  ALG I+G+ I+E +L  I
Sbjct: 425 AQTALRRVEETLQAQLPLDFWTIDLREAIAALGSITGEGIAESMLDLI 454

BLAST of MS018591 vs. ExPASy Swiss-Prot
Match: Q8YN91 (tRNA modification GTPase MnmE OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=mnmE PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 5.6e-114
Identity = 223/467 (47.75%), Postives = 322/467 (68.95%), Query Frame = 0

Query: 84  SSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPTSHVVE 143
           + TIAAI T++     +VGIVR+SG +A+ I  +LF    ++         W   SH + 
Sbjct: 4   TGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFDAPGKQ--------VWE--SHRIL 63

Query: 144 YGAVL-DQQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGARLAE 203
           YG +   Q   ++DE L + M APRSYTREDVVE  CHG  + +++VL+ CLE+GARLA+
Sbjct: 64  YGYIRHPQTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIIAVQQVLQLCLESGARLAQ 123

Query: 204 PGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELL 263
           PGEFTLRAFLNGRLDL+QAE++  L+ A+S  AA  ALAG+QG  +  ++ LR  C+++L
Sbjct: 124 PGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDIL 183

Query: 264 TEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNVG 323
            EIEAR+DF++++PPLD E I+  +  ++ ++   L T +  +LL++G+++AIVGRPNVG
Sbjct: 184 AEIEARIDFEEDLPPLDDEAIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVG 243

Query: 324 KSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKIGVER 383
           KSSLLNAWS+S+RAIVT++ GTTRDV+E+ + V GIPV +LDTAGIRET D VEKIGVER
Sbjct: 244 KSSLLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVER 303

Query: 384 SEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPSPNM 443
           S  AA  AD++++TI A  GWTT D  + ++++ +        P++LV+NKID V    +
Sbjct: 304 SRQAANTADLVLLTIDAATGWTTGDQEIYEQVKHR--------PLILVMNKIDLVEKQLI 363

Query: 444 DDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQCEQLLRT 503
             L    ++  + V+T A   QGI++LE AI E+V   K  A+     +NQRQ   L + 
Sbjct: 364 TSLEYP-ENITQIVHTAAAQKQGIDSLETAILEIVQTGKVQAADMDLAINQRQAAALTQA 423

Query: 504 KEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI 550
           K +L +++++I ++LPLDFWT+DLRGA  ALG+I+G++++E VL  I
Sbjct: 424 KMSLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRI 451

BLAST of MS018591 vs. ExPASy Swiss-Prot
Match: B0CBB0 (tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=mnmE PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.1e-113
Identity = 227/468 (48.50%), Postives = 315/468 (67.31%), Query Frame = 0

Query: 85  STIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPTSHVVEY 144
           +TIAAI T++     ++GIVRLSG  AV+I   LF+   +++        W   SH V Y
Sbjct: 4   TTIAAIATAIVPQQGSIGIVRLSGVEAVSIAKQLFQTPGKQQ--------WE--SHRVLY 63

Query: 145 GAV---LDQQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGARLA 204
           G +   L QQ  +IDE L + MLAPRSYTREDVVE  CHG  + +++VL+ACL+AGA LA
Sbjct: 64  GYIQQPLTQQ--IIDEGLLLLMLAPRSYTREDVVEFHCHGGMIAVQQVLEACLQAGAELA 123

Query: 205 EPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIEL 264
           +PGEFTLRAFLNGRLDL+QAE V  L+ A+S  AA AALAG+QG  +S ++ LR +C++ 
Sbjct: 124 QPGEFTLRAFLNGRLDLTQAEGVADLVGARSPQAAQAALAGVQGKLASPIRELRQRCLDT 183

Query: 265 LTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNV 324
           L E+EAR+DF+D++PPLD   +  ++  +   ++  L TA+  +LL++G+ +AI+GRPNV
Sbjct: 184 LAEVEARVDFEDDLPPLDEAGVQAELQDIHATLQAILATADQGELLRNGLTVAIIGRPNV 243

Query: 325 GKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKIGVE 384
           GKSSLLNAW R +RAIVT++ GTTRDV+E+ + V GIP+ +LDTAGIRET+D VE+IGV 
Sbjct: 244 GKSSLLNAWCRCDRAIVTDLPGTTRDVVESQLVVGGIPIQVLDTAGIRETEDQVEQIGVT 303

Query: 385 RSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPSPN 444
           RS  AA  AD++++TI A  GWT++D  L    Q          P++L++NK+D VP   
Sbjct: 304 RSHQAAQSADLVLLTIDASVGWTSDDQQLYQAFQD--------LPLILIVNKVDLVPQEQ 363

Query: 445 MDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQCEQLLR 504
           +    +  ++  + V T A   QGI  LE AI E V      A+   W +NQRQ   L +
Sbjct: 364 V----VYPEAIAQVVSTIAAQNQGISELETAILETVQTQSLKAANLDWAINQRQAAALQK 423

Query: 505 TKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI 550
            + AL  ++ +I ++LPLDFWT+DLRGA  ALG+I+G+DI+E VL  I
Sbjct: 424 AQAALEHVQGAIADQLPLDFWTIDLRGAIQALGEITGEDITESVLDRI 447

BLAST of MS018591 vs. ExPASy Swiss-Prot
Match: Q3MBM5 (tRNA modification GTPase MnmE OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=mnmE PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.2e-113
Identity = 223/467 (47.75%), Postives = 319/467 (68.31%), Query Frame = 0

Query: 84  SSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPTSHVVE 143
           + TIAAI T++     +VGIVR+SG +A+ I  +LF    ++         W   SH + 
Sbjct: 8   TGTIAAIATAIVPQQGSVGIVRVSGSQAIAIAQTLFHAPGKQ--------VWE--SHRIL 67

Query: 144 YGAVL-DQQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGARLAE 203
           YG +   Q   ++DE L + M APRSYTREDVVE  CHG  + +++VL+ CLE GARLA+
Sbjct: 68  YGYIRHPQTRQIVDEALLLLMKAPRSYTREDVVEFHCHGGIMAVQQVLQLCLEGGARLAQ 127

Query: 204 PGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQCIELL 263
           PGEFTLRAFLNGRLDL+QAE++  L+ A+S  AA  ALAG+QG  +  ++ LR  C+++L
Sbjct: 128 PGEFTLRAFLNGRLDLTQAESIADLVGARSPQAAQTALAGLQGKLAHPIRQLRANCLDIL 187

Query: 264 TEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNVG 323
            EIEAR+DF++++PPLD E I+  +  ++ ++   L T +  +LL++G+++AIVGRPNVG
Sbjct: 188 AEIEARIDFEEDLPPLDDEKIISDIENIAAEISQLLATKDKGELLRTGLKVAIVGRPNVG 247

Query: 324 KSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKIGVER 383
           KSSLLNAWS+S+RAIVT++ GTTRDV+E+ + V GIPV +LDTAGIRET D VEKIGVER
Sbjct: 248 KSSLLNAWSQSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETSDQVEKIGVER 307

Query: 384 SEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPSPNM 443
           S  AA  AD++++TI A  GWTT D  + ++++ +        P++LV+NKID V    +
Sbjct: 308 SRQAANTADLVLLTIDAATGWTTGDQEIYEQVKHR--------PLILVMNKIDLVDKKLI 367

Query: 444 DDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQCEQLLRT 503
             L     +  + V+T A   QGI+ LE AI E+V   K  A+     +NQRQ   L + 
Sbjct: 368 TSLEYP-KNITQIVHTAAAQKQGIDALETAILEIVQTGKVKAADMDLAINQRQAAALTQA 427

Query: 504 KEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI 550
           K +L +++++I ++LPLDFWT+DLRGA  ALG+I+G++++E VL  I
Sbjct: 428 KISLEQVQATITQQLPLDFWTIDLRGAIQALGEITGEEVTESVLDRI 455

BLAST of MS018591 vs. ExPASy TrEMBL
Match: A0A6J1CL38 (uncharacterized protein LOC111011948 OS=Momordica charantia OX=3673 GN=LOC111011948 PE=3 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.6e-303
Identity = 547/549 (99.64%), Postives = 548/549 (99.82%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP
Sbjct: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60

Query: 61  KHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFR 120
           KHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFR
Sbjct: 61  KHNALVLTRDERLENSRAEQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFR 120

Query: 121 PAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCH 180
           PAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCH
Sbjct: 121 PAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCH 180

Query: 181 GSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAAL 240
           GSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA AAL
Sbjct: 181 GSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAAHAAL 240

Query: 241 AGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALET 300
           AGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLEL+MDKVHAMSQDVETALET
Sbjct: 241 AGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELVMDKVHAMSQDVETALET 300

Query: 301 ANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPV 360
           ANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPV
Sbjct: 301 ANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPV 360

Query: 361 TLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKS 420
           TLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKS
Sbjct: 361 TLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKS 420

Query: 421 NGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLD 480
           NGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLD
Sbjct: 421 NGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLD 480

Query: 481 KTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDD 540
           KTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDD
Sbjct: 481 KTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDD 540

Query: 541 ISEEVLSNI 550
           ISEEVLSNI
Sbjct: 541 ISEEVLSNI 549

BLAST of MS018591 vs. ExPASy TrEMBL
Match: A0A6J1JUP0 (uncharacterized protein LOC111489063 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489063 PE=3 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 8.3e-262
Identity = 484/555 (87.21%), Postives = 509/555 (91.71%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHF RT+P MAFL TH H P+S P SL HIP  S+PVLPKSL KSHA+ 
Sbjct: 1   MALLPGFRHFITHFCRTTPPMAFLFTHFHTPISRPSSLFHIPNPSSPVLPKSLIKSHASG 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K     LTRDERLE+S A    EQIENSSTIAAIVTSMGG PAAVGIVRLSGPRAV IVG
Sbjct: 61  KKTTFFLTRDERLEDSHAGIEREQIENSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFRPAARKKTKNLS-PHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVV 180
           SLF PAA+KK KN+S  H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+
Sbjct: 121 SLFCPAAKKKGKNVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVE 300
           ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPL+LE IM+KVHAM+Q+VE
Sbjct: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 CGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQ 420
            GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWT EDTILLD I 
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420

Query: 421 SKKKSNGSATPILLVINKIDCVPS-PNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAIS 480
           +KKKS+GS TPILLVINKIDC PS  NMD + +N DSF+KQV+TCAVTGQGIE+LEMAIS
Sbjct: 421 AKKKSDGSTTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480

Query: 481 ELVGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALG 540
           EL+GL+ TLASGRRWTVNQRQCEQLLRTKEAL RLKSSIEEELPLDFWTVDLRGA LALG
Sbjct: 481 ELMGLNNTLASGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540

Query: 541 QISGDDISEEVLSNI 550
           +ISG+DISEEVLSNI
Sbjct: 541 EISGEDISEEVLSNI 555

BLAST of MS018591 vs. ExPASy TrEMBL
Match: A0A6J1FFU5 (uncharacterized protein LOC111445335 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445335 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.3e-258
Identity = 479/555 (86.31%), Postives = 507/555 (91.35%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFITHF RT+P M FLLTH + P+S P SL  IP  S PVLPKSL KSHA+ 
Sbjct: 1   MALLPGFRHFITHFCRTTPPMPFLLTHFYTPISRPSSLFRIPNPSCPVLPKSLIKSHASG 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K     LTRDERLE+S A    EQIE SSTIAAIVTSMGG PAAVGIVRLSGPRAV IVG
Sbjct: 61  KETTFFLTRDERLEDSHAGIEREQIETSSTIAAIVTSMGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFRPAARKKTKNLS-PHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVV 180
           SLF PAA+KK K++S  H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+
Sbjct: 121 SLFCPAAKKKGKHVSQQHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVI 180

Query: 181 ELQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240
           E+QCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA
Sbjct: 181 EIQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAA 240

Query: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVE 300
           ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPL+LE IM+KVHAM+Q+VE
Sbjct: 241 ADAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLNLETIMEKVHAMAQEVE 300

Query: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTV 360
           TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV
Sbjct: 301 TALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTV 360

Query: 361 CGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQ 420
            GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWT EDTILLD I 
Sbjct: 361 SGIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTEEDTILLDSIL 420

Query: 421 SKKKSNGSATPILLVINKIDCVPS-PNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAIS 480
           +KKKS+GS+TPILLVINKIDC PS  NMD + +N DSF+KQV+TCAVTGQGIE+LEMAIS
Sbjct: 421 AKKKSDGSSTPILLVINKIDCAPSAANMDAISINRDSFSKQVFTCAVTGQGIEHLEMAIS 480

Query: 481 ELVGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALG 540
           EL+GL+ TLA+GRRWTVNQRQCEQLLRTKEAL RLKSSIEEELPLDFWTVDLRGA LALG
Sbjct: 481 ELMGLNNTLATGRRWTVNQRQCEQLLRTKEALGRLKSSIEEELPLDFWTVDLRGAVLALG 540

Query: 541 QISGDDISEEVLSNI 550
           +ISG+DISEEVLSNI
Sbjct: 541 EISGEDISEEVLSNI 555

BLAST of MS018591 vs. ExPASy TrEMBL
Match: A0A0A0KTK4 (TrmE-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G396040 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.0e-256
Identity = 471/553 (85.17%), Postives = 499/553 (90.24%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFI HFYRT+P MAFL TH   P+S P S+  I K+SN VL KSL KSH+T 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLFTHFSTPISRPSSIYSISKTSNHVLSKSLIKSHSTG 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K N  VL  DERL +S A    EQI+NSSTIAAIVTS+GG PAAVGIVRLSGPRAV IVG
Sbjct: 61  KENTFVLAPDERLGDSHAGTEREQIDNSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFRPAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVE 180
           +LF PAA+KK KNLS H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+E
Sbjct: 121 TLFFPAAKKKGKNLSLHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVET 300
           DAALAGIQGGFSSLVK LRTQCIELLTEIEARLDFDDEMPPLDL ++M+KVHAMSQ+VET
Sbjct: 241 DAALAGIQGGFSSLVKSLRTQCIELLTEIEARLDFDDEMPPLDLNIVMEKVHAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVC 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV 
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIM ISALDGWT EDTILL+RI S
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMAISALDGWTAEDTILLNRILS 420

Query: 421 KKKSNGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISEL 480
           KKKS+ S TPILLVINKIDC PSP MD + +N DSF+KQV+TCAVTGQGI+NLEMAISEL
Sbjct: 421 KKKSDESCTPILLVINKIDCAPSPKMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQI 540
           VGL+KTLASGRRWTVNQRQC QLLRTKEA  RLKSSIE+ELP DFWTVDLR A LALG+I
Sbjct: 481 VGLNKTLASGRRWTVNQRQCVQLLRTKEAFTRLKSSIEDELPPDFWTVDLRDAVLALGEI 540

Query: 541 SGDDISEEVLSNI 550
            G+DISEE+LSNI
Sbjct: 541 CGEDISEEILSNI 553

BLAST of MS018591 vs. ExPASy TrEMBL
Match: A0A1S3CCP4 (tRNA modification GTPase MnmE OS=Cucumis melo OX=3656 GN=LOC103499287 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.4e-253
Identity = 467/553 (84.45%), Postives = 495/553 (89.51%), Query Frame = 0

Query: 1   MALLPGFRHFITHFYRTSPSMAFLLTHLHAPVSTPCSLSHIPKSSNPVLPKSLTKSHATP 60
           MALLPGFRHFI HFYRT+P MAFL TH   P+S P SL  I K+SN VL KSL KSH+T 
Sbjct: 1   MALLPGFRHFIAHFYRTTPPMAFLYTHFSTPISRPSSLYSISKTSNHVLSKSLIKSHSTA 60

Query: 61  KHNALVLTRDERLENSRA----EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVG 120
           K N  +L  DERL +S      EQIENSSTIAAIVTS+GG PAAVGIVRLSGPRAV IVG
Sbjct: 61  KENTFLLAPDERLADSHTGIEREQIENSSTIAAIVTSIGGPPAAVGIVRLSGPRAVNIVG 120

Query: 121 SLFRPAARKKTKNLSPHSWRPTSHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVE 180
           +LF PAA+KK KNLS H WRPTSHVVEYG VLDQQGDVIDEVL VPMLAPRSYTREDV+E
Sbjct: 121 TLFCPAAKKKGKNLSRHPWRPTSHVVEYGVVLDQQGDVIDEVLTVPMLAPRSYTREDVIE 180

Query: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240
           LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA
Sbjct: 181 LQCHGSEVCLRRVLKACLEAGARLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAA 240

Query: 241 DAALAGIQGGFSSLVKLLRTQCIELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVET 300
           DAALA IQGGFSSLVKLLR QCIELLTEIEARLDFDDEMPPLDL ++M+KV AMSQ+VET
Sbjct: 241 DAALAAIQGGFSSLVKLLRAQCIELLTEIEARLDFDDEMPPLDLNVVMEKVQAMSQEVET 300

Query: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVC 360
           ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWS+SERAIVTEIAGTTRDVIEANVTV 
Sbjct: 301 ALETANYDKLLQSGIQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEANVTVS 360

Query: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQS 420
           GIPVTLLDTAGIRETDDIVEKIGVERSEAAA GADVIIM ISA DGWT EDTILL+RI S
Sbjct: 361 GIPVTLLDTAGIRETDDIVEKIGVERSEAAAHGADVIIMAISAPDGWTEEDTILLNRILS 420

Query: 421 KKKSNGSATPILLVINKIDCVPSPNMDDLGMNYDSFNKQVYTCAVTGQGIENLEMAISEL 480
           KKKS+ S  PILLV+NKIDC PSPNMD + +N DSF+KQV+TCAVTGQGI+NLEMAISEL
Sbjct: 421 KKKSDESCIPILLVVNKIDCAPSPNMDAMSINRDSFSKQVFTCAVTGQGIQNLEMAISEL 480

Query: 481 VGLDKTLASGRRWTVNQRQCEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQI 540
           VGL+KTLASGRRWTVNQRQCEQLLRTKEA  RLKSSIE+ELP DFWTVDLR A LALG+I
Sbjct: 481 VGLNKTLASGRRWTVNQRQCEQLLRTKEAFTRLKSSIEDELPPDFWTVDLRAAVLALGEI 540

Query: 541 SGDDISEEVLSNI 550
            G+DISEE+LSNI
Sbjct: 541 CGEDISEEILSNI 553

BLAST of MS018591 vs. TAIR 10
Match: AT1G78010.1 (tRNA modification GTPase, putative )

HSP 1 Score: 664.1 bits (1712), Expect = 9.9e-191
Identity = 360/534 (67.42%), Postives = 427/534 (79.96%), Query Frame = 0

Query: 32  VSTPCS--LSHIPKSSNPVLPKSLTKSHA-TPKHNALVLTRDERLEN----------SRA 91
           +S P S  L  +  SS+P    SL K  A + ++N+LV   DER+             + 
Sbjct: 28  ISRPISKNLCFVLFSSSP--KTSLLKPRAQSSENNSLVFKGDERVVGLVGKVVDDAFDKV 87

Query: 92  EQIENSSTIAAIVTSMGGSPAAVGIVRLSGPRAVTIVGSLFRPAARKKTKNLSPHSWRPT 151
           ++ ++SSTI AIVT +GG P AVGIVRLSGP+AV +   +FR A + K K     +WRP 
Sbjct: 88  DRFQSSSTIVAIVTPIGGPPGAVGIVRLSGPKAVEVARRVFRSAKKTKKKESDSDTWRPK 147

Query: 152 SHVVEYGAVLDQQGDVIDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLKACLEAGA 211
           SH VEYGAV+D  G+V+DEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVL+ C+EAGA
Sbjct: 148 SHFVEYGAVVDSNGNVVDEVLAVPMLAPRSYTREDVVELQCHGSEVCLRRVLRTCVEAGA 207

Query: 212 RLAEPGEFTLRAFLNGRLDLSQAENVGKLISAKSTAAADAALAGIQGGFSSLVKLLRTQC 271
           RLAEPGEFTLRAFLNGRLDLSQAENV KLISAKS+AAADAAL GIQGGFSSLVK LR QC
Sbjct: 208 RLAEPGEFTLRAFLNGRLDLSQAENVEKLISAKSSAAADAALEGIQGGFSSLVKSLRAQC 267

Query: 272 IELLTEIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGR 331
           IELLTEIEARLDF+DEMPPLD+E +++K+ +MSQDVE+AL+TANYDKLLQSG+QIAIVGR
Sbjct: 268 IELLTEIEARLDFEDEMPPLDIESVINKITSMSQDVESALDTANYDKLLQSGLQIAIVGR 327

Query: 332 PNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAGIRETDDIVEKI 391
           PNVGKSSLLNAWS+SERAIVTE+AGTTRDV+EANVTV G+P+TLLDTAGIRET+DIVEKI
Sbjct: 328 PNVGKSSLLNAWSKSERAIVTEVAGTTRDVVEANVTVRGVPITLLDTAGIRETNDIVEKI 387

Query: 392 GVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVP 451
           GVERSE AA  ADVIIM +SA++GWT EDT LL +IQS K       P++LV+NKIDC P
Sbjct: 388 GVERSETAAKVADVIIMAVSAVEGWTEEDTELLRKIQSDK-------PMILVMNKIDCAP 447

Query: 452 SPNMD---DLGMNYDSFNKQVYTCAVTGQGIENLEMAISELVGLDKTLASGRRWTVNQRQ 511
             + D   D     + F+K V+T AVTGQGIE LE AI E++GLD+    G +WTVNQRQ
Sbjct: 448 PGSCDQLEDQRKKEEVFHKSVFTSAVTGQGIEELEDAILEILGLDRVPTGGHQWTVNQRQ 507

Query: 512 CEQLLRTKEALARLKSSIEEELPLDFWTVDLRGAALALGQISGDDISEEVLSNI 550
           CEQL+RTKEAL RL+ +IE+E+P+DFWT++LR AAL+L QISG D+SEEVLS+I
Sbjct: 508 CEQLVRTKEALVRLREAIEDEIPIDFWTIELREAALSLAQISGQDVSEEVLSSI 552

BLAST of MS018591 vs. TAIR 10
Match: AT3G12080.1 (GTP-binding family protein )

HSP 1 Score: 96.3 bits (238), Expect = 8.2e-20
Identity = 63/176 (35.80%), Postives = 97/176 (55.11%), Query Frame = 0

Query: 313 IAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVT-VCGIPVTLLDTAGIRET 372
           IAI+GRPNVGKSS+LNA  R +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+ 
Sbjct: 371 IAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKK 430

Query: 373 DDI------VEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSAT 432
             +       E + V R+  A   +DV+ + I A+   T +D  + +RI+ + K      
Sbjct: 431 SSVASSGSTTEAMSVNRAFRAIRRSDVVALVIEAMACITEQDLKIAERIEREGKG----- 490

Query: 433 PILLVINKIDCVPSPNMDDLGMNYDSFNKQ---------VYTCAVTGQGIENLEMA 473
             L+V+NK D +P+ N +      D   ++         VY+ A+TG  ++N+ +A
Sbjct: 491 -CLVVVNKWDTIPNKNQETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVA 540

BLAST of MS018591 vs. TAIR 10
Match: AT3G12080.2 (GTP-binding family protein )

HSP 1 Score: 96.3 bits (238), Expect = 8.2e-20
Identity = 63/176 (35.80%), Postives = 97/176 (55.11%), Query Frame = 0

Query: 313 IAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVT-VCGIPVTLLDTAGIRET 372
           IAI+GRPNVGKSS+LNA  R +R IV+ ++GTTRD I+A  T   G    L+DTAGIR+ 
Sbjct: 371 IAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKK 430

Query: 373 DDI------VEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSAT 432
             +       E + V R+  A   +DV+ + I A+   T +D  + +RI+ + K      
Sbjct: 431 SSVASSGSTTEAMSVNRAFRAIRRSDVVALVIEAMACITEQDLKIAERIEREGKG----- 490

Query: 433 PILLVINKIDCVPSPNMDDLGMNYDSFNKQ---------VYTCAVTGQGIENLEMA 473
             L+V+NK D +P+ N +      D   ++         VY+ A+TG  ++N+ +A
Sbjct: 491 -CLVVVNKWDTIPNKNQETAAHYEDDVREKLRSLKWAPIVYSTAITGHSVDNIVVA 540

BLAST of MS018591 vs. TAIR 10
Match: AT5G39960.1 (GTP binding;GTP binding )

HSP 1 Score: 61.2 bits (147), Expect = 2.9e-09
Identity = 40/128 (31.25%), Postives = 68/128 (53.12%), Query Frame = 0

Query: 311 IQIAIVGRPNVGKSSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIPVTLLDTAG--- 370
           +Q+AIVG+PNVGKS+LLNA    ER +V   AG TRD +       G  V L+DTAG   
Sbjct: 318 LQLAIVGKPNVGKSTLLNALLEEERVLVGPEAGLTRDAVRVQFEFQGRTVYLVDTAGWLE 377

Query: 371 IRETDDIVEKIGVERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPI 430
             E D     + + +S  + + A VI + + A +    + ++    +   +++      +
Sbjct: 378 RTERDKGPASLSIMQSRKSLMRAHVIALVLDAEEIIKAKCSMTHSEVVIARRAVEEGRGL 437

Query: 431 LLVINKID 436
           ++++NK+D
Sbjct: 438 VVIVNKMD 445

BLAST of MS018591 vs. TAIR 10
Match: AT5G66470.1 (RNA binding;GTP binding )

HSP 1 Score: 57.8 bits (138), Expect = 3.2e-08
Identity = 61/221 (27.60%), Postives = 104/221 (47.06%), Query Frame = 0

Query: 264 EIEARLDFDDEMPPLDLELIMDKVHAMSQDVETALETANYDKLLQSGIQIAIVGRPNVGK 323
           E +  +D  DE   L L +  D+  A+  D E   E  +          +A+VG PNVGK
Sbjct: 84  EEQVEIDIVDEASLLSLSMKPDRNMALLDDYEME-ELGHTPNTHHRSGYVAVVGMPNVGK 143

Query: 324 SSLLNAWSRSERAIVTEIAGTTRDVIEANVTVCGIP---VTLLDTAGIRETD-DIVEKIG 383
           S+L N     + +IVT+   TTR  I   + +C  P   + L DT G+ E     ++ + 
Sbjct: 144 STLSNQMIGQKISIVTDKPQTTRHRI---LGICSSPEYQMILYDTPGVIEKKMHRLDTMM 203

Query: 384 VERSEAAALGADVIIMTISALDGWTTEDTILLDRIQSKKKSNGSATPILLVINKIDCVPS 443
           ++    AA+ AD +++ + A    T  + +L + +   +K      P+LLV+NK D +  
Sbjct: 204 MKNVRDAAINADCVVILVDACKTPTNIEEVLKEGLGDLEKK----PPMLLVMNKKDLIKP 263

Query: 444 PNMDDLGMNYDSF---NKQVYTCAVTGQGIENL-EMAISEL 477
             +      Y+ F   ++ +   A  G GIE++ E  +S+L
Sbjct: 264 GEIAKKLEWYEKFTDVDEVIPVSAKYGHGIEDVKEWILSKL 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141643.15.3e-30399.64uncharacterized protein LOC111011948 [Momordica charantia][more]
XP_038874821.18.8e-26687.88tRNA modification GTPase MnmE [Benincasa hispida][more]
XP_022992856.11.7e-26187.21uncharacterized protein LOC111489063 isoform X1 [Cucurbita maxima][more]
XP_023549904.11.0e-25886.13uncharacterized protein LOC111808259 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6579011.11.4e-25886.49hypothetical protein SDJN03_23459, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q8KPU29.2e-11749.36tRNA modification GTPase MnmE OS=Synechococcus elongatus (strain PCC 7942 / FACH... [more]
Q5N6383.0e-11548.93tRNA modification GTPase MnmE OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301... [more]
Q8YN915.6e-11447.75tRNA modification GTPase MnmE OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX ... [more]
B0CBB02.1e-11348.50tRNA modification GTPase MnmE OS=Acaryochloris marina (strain MBIC 11017) OX=329... [more]
Q3MBM56.2e-11347.75tRNA modification GTPase MnmE OS=Trichormus variabilis (strain ATCC 29413 / PCC ... [more]
Match NameE-valueIdentityDescription
A0A6J1CL382.6e-30399.64uncharacterized protein LOC111011948 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1JUP08.3e-26287.21uncharacterized protein LOC111489063 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FFU53.3e-25886.31uncharacterized protein LOC111445335 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0KTK44.0e-25685.17TrmE-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G396040... [more]
A0A1S3CCP41.4e-25384.45tRNA modification GTPase MnmE OS=Cucumis melo OX=3656 GN=LOC103499287 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G78010.19.9e-19167.42tRNA modification GTPase, putative [more]
AT3G12080.18.2e-2035.80GTP-binding family protein [more]
AT3G12080.28.2e-2035.80GTP-binding family protein [more]
AT5G39960.12.9e-0931.25GTP binding;GTP binding [more]
AT5G66470.13.2e-0827.60RNA binding;GTP binding [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 496..516
NoneNo IPR availablePANTHERPTHR42714:SF2TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIALcoord: 84..549
NoneNo IPR availablePANTHERPTHR42714TRNA MODIFICATION GTPASE GTPBP3coord: 84..549
IPR025867MnmE, helical domainPFAMPF12631MnmE_helicalcoord: 218..549
e-value: 3.4E-41
score: 142.1
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 312..433
e-value: 1.2E-23
score: 83.3
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 310..467
e-value: 1.8E-22
score: 77.9
IPR018948GTP-binding protein TrmE, N-terminalPFAMPF10396TrmE_Ncoord: 86..215
e-value: 8.8E-38
score: 129.3
IPR027266GTP-binding protein TrmE/Glycine cleavage system T protein, domain 1GENE3D3.30.1360.120Probable tRNA modification gtpase trme; domain 1coord: 71..215
e-value: 2.2E-45
score: 155.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 311..485
e-value: 2.0E-89
score: 302.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 168..473
IPR027368tRNA modification GTPase MnmE domain 2GENE3D1.20.120.430tRNA modification GTPase MnmE domain 2coord: 216..549
e-value: 2.0E-89
score: 302.7
IPR004520tRNA modification GTPase MnmETIGRFAMTIGR00450TIGR00450coord: 99..549
e-value: 7.8E-112
score: 372.5
IPR004520tRNA modification GTPase MnmEHAMAPMF_00379GTPase_MnmEcoord: 85..549
score: 28.78879
IPR031168TrmE-type guanine nucleotide-binding domainPROSITEPS51709G_TRMEcoord: 310..478
score: 63.173916
IPR031168TrmE-type guanine nucleotide-binding domainCDDcd04164trmEcoord: 308..478
e-value: 5.07021E-67
score: 212.354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018591.1MS018591.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0005515 protein binding