MS018587 (gene) Bitter gourd (TR) v1

Overview
NameMS018587
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionvacuolar protein sorting-associated protein 9A-like
Locationscaffold313: 107116 .. 111299 (-)
RNA-Seq ExpressionMS018587
SyntenyMS018587
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAACGCCGACGTTTTTTTGGGTCTGCACGACTTTCTCGAGCGCATGCGTCAACCATCAGCCTCAGATTTCGTCAAATCCATTAAAAGGTAAATTTGTTTTCCATTCGAATGATATGTGCAAAATATAGAATTTTTATTTACTTCAATTAGTGTATTGTGTTCGTTCCTTCAATTATTATCTGATTTGTTTACACGTTAATTACAATGCTTGCTCATTGTTCTTTTTCTAACTCTTCTGTCTTGACCTCGAACTGATTGCTATTGACAATGGCTGATTGTTTGAAAAAGTATTTGCTACTTTGAAGTTCATTTACTTTTGGAGAAAATTTTCGTGGTCATCAATGGAAGAAGTTAAGTTGGGCCTGTGAAGTAGTATAGTTTATTCAAGCTGAAACTCAGTGCCTAATGTGATTTTATGGTTGATGTTGTTACCGTTAGATTGGAGATTAGTAACAATTACTCTATTGATTAGGTTGTCGCTGGAGATGCACTTCATCATTTCGGCCTTCGGTTTTAGATTTCTAGTTGGCACCCACTTTTAGTATTTGAAGATTCAAATTCTTGTCCACCAGAACTTAAACCAGTTGAATCATTTTTTTCGAGAAGGAGAAAAGCACTCTTATAAATGGTCTCACAGACTGCATTCTCTAGCTGGAATAGCAGTTATGGGTCTCTGAAGTAAAAAGATTTATAGCTAGTTCCTAGTGGCACAGTTCAGTAATTATGTTATATGCAAGCTTCTTATCTAAAAAAATTATGTTATCTGCAAGATGAATCCAATGCTTATACAAGAGTAGCTTCATGAGGTCGGCATAATGGTAAAGCCATTGGATACCTGTTGGTCATTAATTGAAATACCTTTGATACCACATACTTGTGATAGTAAAATACCTTATTGACTACCTAATGCTCTGGAGTTGAGGTGGTTTCAAATTTAAACCCGACGCATAGAGTATATACATGCATAGATGTGTATGTGTGAGTATGTTTCAGTTTGTCAATTTTCAGATTGAAATTGTTACTTTATAATAAAAGAGTTTGGCAGTGATTACTCACGTTGATTATGTTCTCTCTGGAGAAGGAGATAAATCTTTCTCCTGCATATATTTTGCTTGATTATAGGATATCGAATGTTGATTTTCAACTAAACTATAAAACTGCATAAAGTTTAGACTGAACATTATGTACTGGCAGCTTTATTGTGTCATTTTCGAACAATGCTCCTGATCCTGAGAGGGACAGTGCTTCAGTACAAGAATTCTTTGCTAAAATGGAGGGAGCTTTCAGAGCTCATCCGCTTTGGTCCGGTTGCTCAGAGGAAGAGCTCGAAAGTGCTGGTGAGGTAAGTTTACTACTTTTGAGCATGTGTAAATCTTTATAATTAATGATGATTTGTTTTTCATCTCAATAACGAGGAGAATGAGGTGAATGATTAATTTTTTTAGGGTAAAAGGTCTGGACAAAAAGACTATAGTTTTAAAACATCTTTATGTATAATCCATTTGCTATAGTTAAACATGATGGTAAAACAAGCCTACCTGATTAAACGAATAACGCATATTGTGTTCCTGTCCTGTCCTTGTATATTTAACAAATTAACAAAATAAATGTTATAGAAAAGACCAAGATATTTTATCTTGGAGCATATATTTGGAAAACTCTGCAAGTAGTCTTCCCAAATGAACATATTATGTATTCATCATAACATATCTTTGCATCCAAATTTGTTGCTTAAATGATATTTATTATTTCTAATATTAATTCTCTTTCTCATGCACCCCCTTCTTTTTTATACAGGGACTGGAGAAGTATGTCATGACAAAGTTATTTACTCGCGTATATGCTTCACTTCGAGATGATGTAAAAATTGACGAACAACTTTCTGATAAAATTGCATTGATTCAACAGTTTGTTAGGCCAGAAAATTTGGATATCAAACCGAATTTCCAAAATGAAACATCATGGTTGGTGAGTAAAGGTCAAAGTCAAGTGATCTCCATATAATTTGTACTGCAATCATTTTCTCTCTCTTATAGTTTAGATCCTCATGATTGTTTGTTTGGGCATAAATCATACTTCAAAGTTATTTGATATTGTACATTTTGCGAGCATTTTTAAGAACCATATTTATTAGTTTAAATTGGGAAGTATCTTCTCTCCATTATCTTCTCATCTATGAAACATTTACATGATAGATCTTTTCTATTTAAAGATACAAATATACAATAAAAATGTCTACAAAAAAACTTTCTATAGGCTTGTTGTTTTCTCGTCTTGAGATGACAAAAGTGTAGCTTTTTTGATCAGCTGATTCCTATTCATAATGGAAAGGAAATAAGACAAACATTAAAATGTTGTGGATATTTACTTCTAATGATTATGAAATCCCATTGGGTTCTGTAATGCATGACGGGTCTGATTTAAACTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGACTTAAGTTTTTGGTTTTCCATGAAAAAGGTGGGAGAAAGACGGAAACATGAAACCTTTGGTGGTGCGTTCTTGCTATGGAGATTTGAACAAAGTTTCTTCAACTTAGGCCAGTTCTTATTCTCATATCTGACAACATTGTTTCTTTACTTCAAAGTTTGTTTCTCAAGTATTCTATTTTATAACCGAAACTTTCATTTTTTTGAACAAGGTCATGACTCACATTTTAGTATGAGGAATTTTCAACCATCTGAATCTTTGTTAATAATTAATACAAGTCTCACATGCATGTTTACATTTTTATTTGCAGCTTGCTCAGAAAGAACTCCTGAAGATTAACATGTACAAAGCTCCAAGGGACAAACTTGTGTGTCTCCTCAATTGTTGTAAGGTCATCAGTAACTTACTAATTAATGCTTCCGTTGCTTCAAATGAGAATCCTCCCGGGGCTGATGAATTCCTTCCTGTCCTTATTTATGTTATCATAAAGGTGATGTATAATATGGTTACTTTAAATTAGTTTTTTCGTATTCAATAATGTGATTACTTATGAAGCAATGTTTGTGCTAGAGCATATATTCTTACATGTCAGCATTTGAGCATATGATTTCTCCTCATATAATGCCACTGAAAATCTCTAATAATCTCTAATTGAAATTCAACTGAAAAGTTGGCGGTAAAAACAGCACAAAATTCTCTGTCATATTTTATTATATAGTCATTTTGGTATAAAATGTGGGAATTTATCACACCATTCTCATAAATGTGTTTTTCATGCACGATCTCTTGTAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAACGATACAGGGGACAAAATCGATTAACTGGAGAAGCAGCATATTTTTTCACAAACGTACTTTCTGCTGAGTCCTTTATCTCAAACATTGATGCAAAAGCCCTCTCAATGGATGAAATTGAGTTTGAAAATAACATGGAATCTGCTCGAGCACTTCTATCTGGTCTCTCTACTGATGCTGAAGCTATCTCTAATAAAAGTAACTTGGATGAAGGGGTTGTCCGCAATTCAGGACTTGTAAAATCCAGTTCTCAAGCTTCAAATGTTGGTACTGAACCTGCAGTTATACCCAAATCCGCAGAAGCAAAGCCCAGAACTGAGGCATCATGTGCCAAAGATCAGTCGACGGTAATGGAAGAACCATCGCTCTCAGATTTGGAGAATAAGGGTGCAGCCATTCTTTTAAACGATCACACAGGGAGGCAAGCCTTCAGAGATTATCCATATTTATTTGCCCAAGTTGGGGATCTTACATTCAATGACGTAGATGAGCTGCTCAGTCAATACAAACAACTAGTTTTCAAGTACATTTCTCTTTCTAAAGGATTGAGTCTGACGACTTCATCCGTTTCTGTGTCACATTCTGAAATGCAATCCCATCATCGTCCTGAATCTTTCAAAGAACCTGAAGATATTAGAGATGTGACGTCAAATGAGGAGTCAGCTGGAGAGACACATAAGAGAATTGATGGCCCAGATGGAATCTCACTGTTTGGAGAAGAAAGCGTTGGATCAGGGTTACCACAAGACAAGGTAGTACTACTTCAACATGAAGGAAACGACAAGATACCCCAATCC

mRNA sequence

ATGGAGAACGCCGACGTTTTTTTGGGTCTGCACGACTTTCTCGAGCGCATGCGTCAACCATCAGCCTCAGATTTCGTCAAATCCATTAAAAGCTTTATTGTGTCATTTTCGAACAATGCTCCTGATCCTGAGAGGGACAGTGCTTCAGTACAAGAATTCTTTGCTAAAATGGAGGGAGCTTTCAGAGCTCATCCGCTTTGGTCCGGTTGCTCAGAGGAAGAGCTCGAAAGTGCTGGTGAGGGACTGGAGAAGTATGTCATGACAAAGTTATTTACTCGCGTATATGCTTCACTTCGAGATGATGTAAAAATTGACGAACAACTTTCTGATAAAATTGCATTGATTCAACAGTTTGTTAGGCCAGAAAATTTGGATATCAAACCGAATTTCCAAAATGAAACATCATGGTTGCTTGCTCAGAAAGAACTCCTGAAGATTAACATGTACAAAGCTCCAAGGGACAAACTTGTGTGTCTCCTCAATTGTTGTAAGGTCATCAGTAACTTACTAATTAATGCTTCCGTTGCTTCAAATGAGAATCCTCCCGGGGCTGATGAATTCCTTCCTGTCCTTATTTATGTTATCATAAAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAACGATACAGGGGACAAAATCGATTAACTGGAGAAGCAGCATATTTTTTCACAAACGTACTTTCTGCTGAGTCCTTTATCTCAAACATTGATGCAAAAGCCCTCTCAATGGATGAAATTGAGTTTGAAAATAACATGGAATCTGCTCGAGCACTTCTATCTGGTCTCTCTACTGATGCTGAAGCTATCTCTAATAAAAGTAACTTGGATGAAGGGGTTGTCCGCAATTCAGGACTTGTAAAATCCAGTTCTCAAGCTTCAAATGTTGGTACTGAACCTGCAGTTATACCCAAATCCGCAGAAGCAAAGCCCAGAACTGAGGCATCATGTGCCAAAGATCAGTCGACGGTAATGGAAGAACCATCGCTCTCAGATTTGGAGAATAAGGGTGCAGCCATTCTTTTAAACGATCACACAGGGAGGCAAGCCTTCAGAGATTATCCATATTTATTTGCCCAAGTTGGGGATCTTACATTCAATGACGTAGATGAGCTGCTCAGTCAATACAAACAACTAGTTTTCAAGTACATTTCTCTTTCTAAAGGATTGAGTCTGACGACTTCATCCGTTTCTGTGTCACATTCTGAAATGCAATCCCATCATCGTCCTGAATCTTTCAAAGAACCTGAAGATATTAGAGATGTGACGTCAAATGAGGAGTCAGCTGGAGAGACACATAAGAGAATTGATGGCCCAGATGGAATCTCACTGTTTGGAGAAGAAAGCGTTGGATCAGGGTTACCACAAGACAAGGTAGTACTACTTCAACATGAAGGAAACGACAAGATACCCCAATCC

Coding sequence (CDS)

ATGGAGAACGCCGACGTTTTTTTGGGTCTGCACGACTTTCTCGAGCGCATGCGTCAACCATCAGCCTCAGATTTCGTCAAATCCATTAAAAGCTTTATTGTGTCATTTTCGAACAATGCTCCTGATCCTGAGAGGGACAGTGCTTCAGTACAAGAATTCTTTGCTAAAATGGAGGGAGCTTTCAGAGCTCATCCGCTTTGGTCCGGTTGCTCAGAGGAAGAGCTCGAAAGTGCTGGTGAGGGACTGGAGAAGTATGTCATGACAAAGTTATTTACTCGCGTATATGCTTCACTTCGAGATGATGTAAAAATTGACGAACAACTTTCTGATAAAATTGCATTGATTCAACAGTTTGTTAGGCCAGAAAATTTGGATATCAAACCGAATTTCCAAAATGAAACATCATGGTTGCTTGCTCAGAAAGAACTCCTGAAGATTAACATGTACAAAGCTCCAAGGGACAAACTTGTGTGTCTCCTCAATTGTTGTAAGGTCATCAGTAACTTACTAATTAATGCTTCCGTTGCTTCAAATGAGAATCCTCCCGGGGCTGATGAATTCCTTCCTGTCCTTATTTATGTTATCATAAAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAACGATACAGGGGACAAAATCGATTAACTGGAGAAGCAGCATATTTTTTCACAAACGTACTTTCTGCTGAGTCCTTTATCTCAAACATTGATGCAAAAGCCCTCTCAATGGATGAAATTGAGTTTGAAAATAACATGGAATCTGCTCGAGCACTTCTATCTGGTCTCTCTACTGATGCTGAAGCTATCTCTAATAAAAGTAACTTGGATGAAGGGGTTGTCCGCAATTCAGGACTTGTAAAATCCAGTTCTCAAGCTTCAAATGTTGGTACTGAACCTGCAGTTATACCCAAATCCGCAGAAGCAAAGCCCAGAACTGAGGCATCATGTGCCAAAGATCAGTCGACGGTAATGGAAGAACCATCGCTCTCAGATTTGGAGAATAAGGGTGCAGCCATTCTTTTAAACGATCACACAGGGAGGCAAGCCTTCAGAGATTATCCATATTTATTTGCCCAAGTTGGGGATCTTACATTCAATGACGTAGATGAGCTGCTCAGTCAATACAAACAACTAGTTTTCAAGTACATTTCTCTTTCTAAAGGATTGAGTCTGACGACTTCATCCGTTTCTGTGTCACATTCTGAAATGCAATCCCATCATCGTCCTGAATCTTTCAAAGAACCTGAAGATATTAGAGATGTGACGTCAAATGAGGAGTCAGCTGGAGAGACACATAAGAGAATTGATGGCCCAGATGGAATCTCACTGTTTGGAGAAGAAAGCGTTGGATCAGGGTTACCACAAGACAAGGTAGTACTACTTCAACATGAAGGAAACGACAAGATACCCCAATCC

Protein sequence

MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVRPENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNIDAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVGTEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYLFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPEDIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS
Homology
BLAST of MS018587 vs. NCBI nr
Match: XP_022141647.1 (vacuolar protein sorting-associated protein 9A-like [Momordica charantia])

HSP 1 Score: 916.4 bits (2367), Expect = 1.0e-262
Identity = 473/476 (99.37%), Postives = 473/476 (99.37%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DAKALSMDEIEFEN MESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE
Sbjct: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 477
            IRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS
Sbjct: 421 GIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 476

BLAST of MS018587 vs. NCBI nr
Match: XP_023525567.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 409/471 (86.84%), Postives = 435/471 (92.36%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL++RVYASL DDVKIDEQLSDK+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLL+NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DA ALSM+E EFE NMESARALLSGLSTD E+ SNKSNLDE V RNS LV++SSQASN G
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAV P S E+K  TE    K QSTVME  +LSDLENKGAA+LLNDHT +QAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDV+ELL+QY+ LVFKY+SLSKGLSL +SS S S+SEM SHH PESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGND 472
           D+RDVTSN+ESAGETH  IDGPDG S FGEE+VGSGL QDKVVL QHEGND
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGND 471

BLAST of MS018587 vs. NCBI nr
Match: XP_022990551.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima] >XP_022990552.1 vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima])

HSP 1 Score: 791.6 bits (2043), Expect = 3.8e-225
Identity = 406/471 (86.20%), Postives = 434/471 (92.14%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERD ASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL++RVYASL DDVKIDEQLSDK+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLL+NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DA +LSM+E EFE NMESARALLSGLSTD E+ SNKSNLDE V RNS LV++SSQASN G
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAV P S E+KP TE    K QSTVME  +LSDLE+KGAA+LLNDHT +QAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDV+ELL+QY+ +VFKY+SLSKGLSL +SS S S SEM SHH PESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGND 472
           D+RDVTSN+ESAGETH  IDGPDG SLFGEE+VGSGL QDKVVL QHEGND
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGND 471

BLAST of MS018587 vs. NCBI nr
Match: XP_038874663.1 (vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_038874664.1 vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_038874665.1 vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_038874667.1 vacuolar protein sorting-associated protein 9A-like [Benincasa hispida])

HSP 1 Score: 791.2 bits (2042), Expect = 5.0e-225
Identity = 411/477 (86.16%), Postives = 441/477 (92.45%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLF+RVYASL DDVKIDEQLS+K+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL+CCKVISNLL NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFI NI
Sbjct: 181 LPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFILNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DAKALSM+EIEFE NMESA+ALLSGLS+D EA+SNKSNL+EGVV +S LV++SSQAS   
Sbjct: 241 DAKALSMEEIEFEKNMESAQALLSGLSSDVEALSNKSNLNEGVVHSSELVETSSQAS--- 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHT-GRQAFRDYPY 360
            EPAV PKS EAKPRTEA  AKDQS VME PSLSDLENKGAAILLND T GRQAFRDYPY
Sbjct: 301 VEPAVRPKSTEAKPRTEAPLAKDQSAVMEAPSLSDLENKGAAILLNDQTGGRQAFRDYPY 360

Query: 361 LFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEP 420
           LF+QVGDLTF DV+ELLSQYKQLVFKY+SLS+GLS+TT+  S S+SEM S H PESFKEP
Sbjct: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSVTTAFSSYSNSEMHSQHHPESFKEP 420

Query: 421 EDIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 477
           ED+RDVTSN+ESAGE H+ IDG DG SLFGEE+V SGLPQDKVVL QHEGN++  QS
Sbjct: 421 EDVRDVTSNDESAGERHRLIDGSDGTSLFGEENVRSGLPQDKVVLPQHEGNEETSQS 474

BLAST of MS018587 vs. NCBI nr
Match: XP_022955656.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata] >XP_022955669.1 vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata])

HSP 1 Score: 788.5 bits (2035), Expect = 3.2e-224
Identity = 407/471 (86.41%), Postives = 433/471 (91.93%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL++RVYASL DDVKIDEQLSDK+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLL+NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DA ALSM+E EFE NMESARALLSGLSTD E+ SNKSNLDE V RNS LV++SSQASN G
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAV P S E+KP TE    K QSTV E  +LSDLENKGAA+LLNDHT +QAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDV+ELL+QY+ LVFKY+SLSKGLSL +SS S S+SEM SH  PESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHLNPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGND 472
           D+RDVTSN+ESAGETH  IDGPDG S FGEE+VGSGL QDKVVL QHE ND
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEVND 471

BLAST of MS018587 vs. ExPASy Swiss-Prot
Match: Q9LT31 (Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.6e-144
Identity = 280/445 (62.92%), Postives = 339/445 (76.18%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMR+PSA DFVKSIKSFIVSFSNNAPDPE+D A VQEFF+KME A
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEEL+SAG+GLEKYVMTKLFTRV+AS  ++V  DE+L  K++L+QQF+ 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDI+P FQNE+SWLLAQKEL KINMYKAPRDKLVC+LNCCKVI+NLL+NAS+ASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +++L GEAAYFFTN+LSAESFISNI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKS---NLDEGVVRNSGLVKSSSQAS 300
           DAK++S+DE EFE NMESARA +SGL +      + S     DE  ++        +Q+ 
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPRDESTLQ-------KTQSL 300

Query: 301 NVGTEPAVI-PKSAEAKPRTEASCAKDQSTVMEE-PSLSDLENKGAAILLNDHTGRQAFR 360
           N   E  +   KS+++   T      +  T M++  S+SDLENKGA  LL D    + F+
Sbjct: 301 NPKRENTLFQSKSSDSLSGTNELLNINSETPMKKAESISDLENKGAT-LLKDTEPSKVFQ 360

Query: 361 DYPYLFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPES 420
           +YPY+FA  GDL   DV+ LL+ YKQLVFKY+ L+KGL   T S++ S S +Q+     +
Sbjct: 361 EYPYIFASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLGDGT-SLAPSSSPLQASSGFNT 420

Query: 421 FKEPEDIRDVTSNEESAGETHKRID 441
            KE ED R  +S+ +   ET + +D
Sbjct: 421 SKESEDHRRSSSDVQMTKETDRSVD 436

BLAST of MS018587 vs. ExPASy Swiss-Prot
Match: Q10NQ3 (Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica OX=39947 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.2e-133
Identity = 254/428 (59.35%), Postives = 316/428 (73.83%), Query Frame = 0

Query: 8   LGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLW 67
           L  HDFLERMRQPSA+DFVKSIK FIV+FSN APDPE DSA+VQEF   MEGAFRAH  W
Sbjct: 17  LAWHDFLERMRQPSAADFVKSIKGFIVTFSNRAPDPEHDSAAVQEFLENMEGAFRAHTPW 76

Query: 68  SGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVRPENLDIK 127
           +G SEEELESAGEGLEKYVMTKLF RV+AS+ +DVK DE+L +K++L+QQF+RPENLDIK
Sbjct: 77  AGSSEEELESAGEGLEKYVMTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIK 136

Query: 128 PNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNENPPGADEF 187
           P +Q+ETSWLLAQKEL KINMYKAPRDKL C+LNCCKVI+NLL+NAS+ SNENPPGADEF
Sbjct: 137 PEYQSETSWLLAQKELQKINMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEF 196

Query: 188 LPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNIDAKALSM 247
           LPVLIYV IKANPPQLHSNLLYIQRYR Q+RL  EA YFFTN+LSAESFI NID ++LSM
Sbjct: 197 LPVLIYVTIKANPPQLHSNLLYIQRYRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSM 256

Query: 248 DEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVGTEPAVIP 307
           DE +F+  M+ AR  + GLS  +E   N++NLD    ++  L  S     N+  +     
Sbjct: 257 DERDFQKKMDLARERMLGLSASSENQDNQNNLDVREQKSQTLKASRDSDVNLSLKDNFQG 316

Query: 308 KSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYLFAQVGDL 367
              E +  ++AS     + V    S+SDLE KGAA LL D    +  ++YP+LFA+ GDL
Sbjct: 317 PGLEMRRDSDAS----SNPVERVQSISDLEKKGAAELLKDDDLNKKIQEYPFLFARSGDL 376

Query: 368 TFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPEDIRDVTS 427
           T  DV+ LL+ YKQLV KY++LS+G+ +   +  V    MQ+       +EP+++++  +
Sbjct: 377 TVADVENLLNSYKQLVLKYVALSQGMGINLENPPV--QSMQTVSDLVESEEPKNVKNAVN 436

Query: 428 NEESAGET 436
             E + +T
Sbjct: 437 FSEGSSKT 438

BLAST of MS018587 vs. ExPASy Swiss-Prot
Match: O74396 (Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps901 PE=1 SV=3)

HSP 1 Score: 166.4 bits (420), Expect = 7.9e-40
Identity = 102/308 (33.12%), Postives = 167/308 (54.22%), Query Frame = 0

Query: 11  HDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGC 70
           H FLE++R  SA    K +KSF+  F+            +++F   +      +  W+  
Sbjct: 116 HMFLEQLRSSSAEPVAKYLKSFLSEFTKRRWTVNYQVKLIRDFLKFINEKIEQYEPWASG 175

Query: 71  SEEELESAGEGLEKYVMTKLFTRVY------------ASLRDDVKIDEQLSDKIALIQQF 130
           S+ E+++A EG+EK V+ +L+T ++            +   DDV+ D  LS+K+ L  Q+
Sbjct: 176 SQAEIDNAKEGMEKLVLNRLYTSLFSPEIAKSGIPLSSEHSDDVEEDRVLSEKMELF-QW 235

Query: 131 VRPENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASN 190
           +  ENLDIK    +   + LA  EL +IN Y APRDK++CLLNCCKVI + L N  V   
Sbjct: 236 ITEENLDIKKQKSSSKFFKLAADELRRINDYHAPRDKIICLLNCCKVIFSYLRN--VVKE 295

Query: 191 ENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFIS 250
           E+   AD F+P+LI+V+++A P  L SN+ YIQR+R   +LTGE  Y+ + ++ A SFI 
Sbjct: 296 ES---ADMFVPILIFVVLQARPAHLVSNIQYIQRFRSPEKLTGEVMYYLSTLMGAMSFIE 355

Query: 251 NIDAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASN 307
            +D  +L++ E EF   +E +   +       ++ S  +  +    ++  L +  S + +
Sbjct: 356 TLDCSSLTITEEEFNAQIEKSIKKMEERKLSEKSESKTAVNENATYKDPVLSRGLSSSID 415

BLAST of MS018587 vs. ExPASy Swiss-Prot
Match: P54787 (Vacuolar protein sorting-associated protein 9 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS9 PE=1 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 5.0e-26
Identity = 101/346 (29.19%), Postives = 172/346 (49.71%), Query Frame = 0

Query: 7   FLGLHDFLERMRQPSASDFVKSIKSFIVSF--SNNAPDPERDSASVQEFFAKMEGAFRAH 66
           F     F+++++ P A   VK  KSF+ +F           +   + +F   +   F  +
Sbjct: 60  FYDFQIFIKQLQTPGADPLVKYTKSFLRNFLAQRLLWTVSEEIKLISDFKTFIYDKFTLY 119

Query: 67  PLWSGCSEEELESAGEGLEKYVMTKLFTRVYA-SLRD------------DVKIDEQLSDK 126
             +      ++ +A EG+EK +M KL++R ++ SL +            D+  D+ L +K
Sbjct: 120 EPFRSLDNSKMRNAKEGMEKLIMGKLYSRCFSPSLYEILQKPLDDEHMKDLTNDDTLLEK 179

Query: 127 IALIQQFVRPENLDI---KPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISN 186
           I    +F+ P  LDI    PN +      LA KEL KIN +K+PRDK+VC+LN  KVI  
Sbjct: 180 IR-HYRFISPIMLDIPDTMPNARLNKFVHLASKELGKINRFKSPRDKMVCVLNASKVIFG 239

Query: 187 LLINASVASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFT 246
           LL +  +  N    GAD F+PVLIY I+K     L SN+ YI+R+R  + + GE  Y+ +
Sbjct: 240 LLKHTKLEQN----GADSFIPVLIYCILKGQVRYLVSNVNYIERFRSPDFIRGEEEYYLS 299

Query: 247 NVLSAESFISNIDAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLD--EGVVRN 306
           ++ +A +FI N+  ++L+++  + E+  E+ +     L+ + E    K  L+  + +  N
Sbjct: 300 SLQAALNFIMNLTERSLTIE--DHEDFEEAYQRNFKQLAEEKEEEEKKKQLEIPDELQPN 359

Query: 307 SGLVKSSSQASNVGTEPAVIPKSAEAKPRTEASCAKDQSTVMEEPS 333
             L+K   + +N+         S   +P  E +   +QS   E+ S
Sbjct: 360 GTLLKPLDEVTNIVISKFNELFSPIGEPTQEEALKSEQSNKEEDVS 398

BLAST of MS018587 vs. ExPASy Swiss-Prot
Match: O18973 (Rab5 GDP/GTP exchange factor OS=Bos taurus OX=9913 GN=RABGEF1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 9.4e-25
Identity = 73/218 (33.49%), Postives = 122/218 (55.96%), Query Frame = 0

Query: 44  ERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYA-SLRDDV 103
           E  S   Q+F+  +    +          E +E   + +EKY+MT+L+  V+     DD 
Sbjct: 177 EEQSECTQDFYQNVAERMQTR---GKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDE 236

Query: 104 KIDEQLSDKIALIQQFVRPENLDIKPNFQ-NETSWLL--AQKELLKINMYKAPRDKLVCL 163
           K D  +  +I  +  +V P+ L +  N +  E S ++  A  ++++++  + PRDKL C+
Sbjct: 237 KKDLAIQKRIRAL-HWVTPQMLCVPVNEEIPEVSDMVVKAITDIIEMDSKRVPRDKLACI 296

Query: 164 LNCCKVISNLLINASVASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRL 223
             C K I N +    +  NE P  AD+FLP LIY+++K NPP+L SN+ YI R+   +RL
Sbjct: 297 TKCSKHIFNAI---KITKNE-PASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRL 356

Query: 224 -TGEAAYFFTNVLSAESFISNIDAKALSMDEIEFENNM 257
            TGE  Y+FTN+  A +FI  +DA++L++ + +F+  M
Sbjct: 357 MTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYM 386

BLAST of MS018587 vs. ExPASy TrEMBL
Match: A0A6J1CKF0 (vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=3673 GN=LOC111011952 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.0e-263
Identity = 473/476 (99.37%), Postives = 473/476 (99.37%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DAKALSMDEIEFEN MESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE
Sbjct: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 477
            IRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS
Sbjct: 421 GIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 476

BLAST of MS018587 vs. ExPASy TrEMBL
Match: A0A6J1JJ31 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 GN=LOC111487398 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.9e-225
Identity = 406/471 (86.20%), Postives = 434/471 (92.14%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERD ASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL++RVYASL DDVKIDEQLSDK+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLL+NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DA +LSM+E EFE NMESARALLSGLSTD E+ SNKSNLDE V RNS LV++SSQASN G
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAV P S E+KP TE    K QSTVME  +LSDLE+KGAA+LLNDHT +QAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDV+ELL+QY+ +VFKY+SLSKGLSL +SS S S SEM SHH PESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGND 472
           D+RDVTSN+ESAGETH  IDGPDG SLFGEE+VGSGL QDKVVL QHEGND
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGND 471

BLAST of MS018587 vs. ExPASy TrEMBL
Match: A0A6J1GWY3 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita moschata OX=3662 GN=LOC111457590 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.6e-224
Identity = 407/471 (86.41%), Postives = 433/471 (91.93%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL++RVYASL DDVKIDEQLSDK+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLL+NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DA ALSM+E EFE NMESARALLSGLSTD E+ SNKSNLDE V RNS LV++SSQASN G
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360
           TEPAV P S E+KP TE    K QSTV E  +LSDLENKGAA+LLNDHT +QAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420
           FAQVGDLTFNDV+ELL+QY+ LVFKY+SLSKGLSL +SS S S+SEM SH  PESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHLNPESFKEPE 420

Query: 421 DIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGND 472
           D+RDVTSN+ESAGETH  IDGPDG S FGEE+VGSGL QDKVVL QHE ND
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEVND 471

BLAST of MS018587 vs. ExPASy TrEMBL
Match: A0A1S3B4M4 (vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=LOC103485761 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 407/477 (85.32%), Postives = 434/477 (90.99%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 29  MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 88

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLF+RVYASL DDVKIDEQLS+K+ALIQQF+R
Sbjct: 89  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 148

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL+CCKVISNLL NAS+ASNEN
Sbjct: 149 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 208

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 209 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 268

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DAKALSM+EIEFE NMESARALLSGLS+D EAISNK+NLDEGV   S LV++SSQAS   
Sbjct: 269 DAKALSMEEIEFEKNMESARALLSGLSSDVEAISNKNNLDEGVGHYSELVETSSQAS--- 328

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHT-GRQAFRDYPY 360
            E  V PK  E KPRTEA  AKDQSTVME PSLSDLENKGA ILLND T GRQA RDYPY
Sbjct: 329 VESQVRPKPTEGKPRTEAPHAKDQSTVMEVPSLSDLENKGATILLNDQTGGRQALRDYPY 388

Query: 361 LFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEP 420
           LF+QVGDLTF DV+ELLSQYKQLVFKY+SLS+GL LTT   S S+SEM S H  ESFKEP
Sbjct: 389 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLGLTTELSSYSNSEMYSQHHHESFKEP 448

Query: 421 EDIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 477
           ED+RD+TSN+ESAGE H+ IDGPDG SLFGEE+VGSGL  DKVVL QHEGN+++ QS
Sbjct: 449 EDVRDMTSNDESAGEGHRLIDGPDGTSLFGEENVGSGL--DKVVLPQHEGNNEMSQS 500

BLAST of MS018587 vs. ExPASy TrEMBL
Match: A0A0A0LI50 (VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.4e-220
Identity = 404/477 (84.70%), Postives = 433/477 (90.78%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLF+RVYASL DDVKIDEQ+S+K+ALIQQF+R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQISEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDIKPNFQNETSWLLAQKELLKINM+KAPRDKLVCLL+CCKVISNLL NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTNVLSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300
           DAKALSM+EIEFE NMESARALLSGLS+D EA+SNK+NLDEGV   S LV++SSQAS   
Sbjct: 241 DAKALSMEEIEFEKNMESARALLSGLSSDVEALSNKNNLDEGVGHYSELVETSSQAS--- 300

Query: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHT-GRQAFRDYPY 360
            E  V PK  E KPRTEA  AKDQS VM+ PSLSDLENKGA ILLND T GRQA R+YPY
Sbjct: 301 VESPVRPKPTEGKPRTEAPHAKDQSAVMKVPSLSDLENKGATILLNDQTGGRQALREYPY 360

Query: 361 LFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEP 420
           LF+QVGDLTF DV+ELLSQYKQLVFKY+SLS+GLSLTT   S S SEM S H  ESFKEP
Sbjct: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSLTTEFSSYSKSEMHSQHHHESFKEP 420

Query: 421 EDIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGNDKIPQS 477
           ED+RDVTSN+ESAG+ H+ IDGPDG SLFGEE+VGSGLPQDKV L Q EGN+++ QS
Sbjct: 421 EDVRDVTSNDESAGDRHRLIDGPDGTSLFGEENVGSGLPQDKVELPQREGNNEMSQS 474

BLAST of MS018587 vs. TAIR 10
Match: AT3G19770.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 513.5 bits (1321), Expect = 1.9e-145
Identity = 280/445 (62.92%), Postives = 339/445 (76.18%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMR+PSA DFVKSIKSFIVSFSNNAPDPE+D A VQEFF+KME A
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCSEEEL+SAG+GLEKYVMTKLFTRV+AS  ++V  DE+L  K++L+QQF+ 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDI+P FQNE+SWLLAQKEL KINMYKAPRDKLVC+LNCCKVI+NLL+NAS+ASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +++L GEAAYFFTN+LSAESFISNI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKS---NLDEGVVRNSGLVKSSSQAS 300
           DAK++S+DE EFE NMESARA +SGL +      + S     DE  ++        +Q+ 
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPRDESTLQ-------KTQSL 300

Query: 301 NVGTEPAVI-PKSAEAKPRTEASCAKDQSTVMEE-PSLSDLENKGAAILLNDHTGRQAFR 360
           N   E  +   KS+++   T      +  T M++  S+SDLENKGA  LL D    + F+
Sbjct: 301 NPKRENTLFQSKSSDSLSGTNELLNINSETPMKKAESISDLENKGAT-LLKDTEPSKVFQ 360

Query: 361 DYPYLFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPES 420
           +YPY+FA  GDL   DV+ LL+ YKQLVFKY+ L+KGL   T S++ S S +Q+     +
Sbjct: 361 EYPYIFASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLGDGT-SLAPSSSPLQASSGFNT 420

Query: 421 FKEPEDIRDVTSNEESAGETHKRID 441
            KE ED R  +S+ +   ET + +D
Sbjct: 421 SKESEDHRRSSSDVQMTKETDRSVD 436

BLAST of MS018587 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 460.3 bits (1183), Expect = 1.9e-129
Identity = 250/443 (56.43%), Postives = 317/443 (71.56%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DV  GLH+FL +   PSA DF+KSIKSFIVS  N APDPE+D  +VQ+FF KME A
Sbjct: 1   MENTDVVSGLHNFLSK---PSAKDFIKSIKSFIVSILNTAPDPEKDCDAVQDFFYKMESA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFVR 120
           FRAHPLWSGCS++EL++AG+GLEKYVMTKLF RV+AS  +DV  DE+L  KI+L+QQF+ 
Sbjct: 61  FRAHPLWSGCSDDELDNAGDGLEKYVMTKLFPRVFASNTEDVISDEKLFQKISLVQQFIS 120

Query: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180
           PENLDI+P FQN+TSWLLAQKEL KINMY APRDKL+C+L CCKVI+NLL+NAS+ASN+N
Sbjct: 121 PENLDIQPTFQNQTSWLLAQKELQKINMYNAPRDKLMCILRCCKVINNLLLNASIASNQN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240
            PGAD+FLPVLIYV IKANPPQ HSNLLYIQRYR Q++L GEA Y FTN+LSAESFISNI
Sbjct: 181 EPGADQFLPVLIYVTIKANPPQFHSNLLYIQRYRRQSKLVGEAGYLFTNILSAESFISNI 240

Query: 241 DAKALSMDEIEFENNMESARALLSGLSTDAEAISNKSNLD--EGVVRNSGLVKSSSQASN 300
           DAK+LSMDE +FE  M+SA A LSG  + +    + + L       R + L+ + S  S 
Sbjct: 241 DAKSLSMDEADFETKMKSAHARLSGPGSQSYQTDHGAALPTAHNTKRENMLLHTKSTDSF 300

Query: 301 VGTEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHT-GRQAFRDY 360
            GT   +                  ++ + +   ++DLENKGAA LLND +   + F++Y
Sbjct: 301 SGTNETL-----------------SETPIKKADPITDLENKGAATLLNDRSEATKIFQEY 360

Query: 361 PYLFAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFK 420
           PY+FA VGDL   DV++LL+ YKQLVFKY+ LSKGL   T S++   S +Q+       K
Sbjct: 361 PYMFASVGDLKIGDVEDLLNNYKQLVFKYVCLSKGLGDAT-SLTPCISPLQAS------K 416

Query: 421 EPEDIRDVTSNEESAGETHKRID 441
             E+   ++S+ ++  ET + +D
Sbjct: 421 VSENHTTLSSDFQTKSETDRSVD 416

BLAST of MS018587 vs. TAIR 10
Match: AT3G19770.2 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 286.6 bits (732), Expect = 3.7e-77
Identity = 170/309 (55.02%), Postives = 215/309 (69.58%), Query Frame = 0

Query: 137 LLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNENPPGADEFLPVLIYVII 196
           + AQKEL KINMYKAPRDKLVC+LNCCKVI+NLL+NAS+ASNEN PGADEFLPVLIYV I
Sbjct: 1   MAAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNENAPGADEFLPVLIYVTI 60

Query: 197 KANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNIDAKALSMDEIEFENNM 256
           KANPPQLHSNLLYIQRYR +++L GEAAYFFTN+LSAESFISNIDAK++S+DE EFE NM
Sbjct: 61  KANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSISLDEAEFEKNM 120

Query: 257 ESARALLSGLSTDAEAISNKS---NLDEGVVRNSGLVKSSSQASNVGTEPAVI-PKSAEA 316
           ESARA +SGL +      + S     DE  ++        +Q+ N   E  +   KS+++
Sbjct: 121 ESARARISGLDSQTYQTGHGSAPPPRDESTLQ-------KTQSLNPKRENTLFQSKSSDS 180

Query: 317 KPRTEASCAKDQSTVMEE-PSLSDLENKGAAILLNDHTGRQAFRDYPYLFAQVGDLTFND 376
              T      +  T M++  S+SDLENKGA  LL D    + F++YPY+FA  GDL   D
Sbjct: 181 LSGTNELLNINSETPMKKAESISDLENKGAT-LLKDTEPSKVFQEYPYIFASAGDLRIGD 240

Query: 377 VDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPEDIRDVTSNEES 436
           V+ LL+ YKQLVFKY+ L+KGL   T S++ S S +Q+     + KE ED R  +S+ + 
Sbjct: 241 VEGLLNSYKQLVFKYVCLTKGLGDGT-SLAPSSSPLQASSGFNTSKESEDHRRSSSDVQM 300

Query: 437 AGETHKRID 441
             ET + +D
Sbjct: 301 TKETDRSVD 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141647.11.0e-26299.37vacuolar protein sorting-associated protein 9A-like [Momordica charantia][more]
XP_023525567.12.6e-22686.84vacuolar protein sorting-associated protein 9A-like [Cucurbita pepo subsp. pepo][more]
XP_022990551.13.8e-22586.20vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima] >XP_02299... [more]
XP_038874663.15.0e-22586.16vacuolar protein sorting-associated protein 9A-like [Benincasa hispida] >XP_0388... [more]
XP_022955656.13.2e-22486.41vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata] >XP_022... [more]
Match NameE-valueIdentityDescription
Q9LT312.6e-14462.92Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 G... [more]
Q10NQ31.2e-13359.35Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica O... [more]
O743967.9e-4033.12Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (str... [more]
P547875.0e-2629.19Vacuolar protein sorting-associated protein 9 OS=Saccharomyces cerevisiae (strai... [more]
O189739.4e-2533.49Rab5 GDP/GTP exchange factor OS=Bos taurus OX=9913 GN=RABGEF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CKF05.0e-26399.37vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=36... [more]
A0A6J1JJ311.9e-22586.20vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1GWY31.6e-22486.41vacuolar protein sorting-associated protein 9A-like OS=Cucurbita moschata OX=366... [more]
A0A1S3B4M41.1e-22085.32vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0LI501.4e-22084.70VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G19770.11.9e-14562.92Vacuolar sorting protein 9 (VPS9) domain [more]
AT5G09320.11.9e-12956.43Vacuolar sorting protein 9 (VPS9) domain [more]
AT3G19770.23.7e-7755.02Vacuolar sorting protein 9 (VPS9) domain [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003123VPS9 domainSMARTSM00167vps9_2coord: 135..254
e-value: 5.0E-30
score: 115.7
IPR003123VPS9 domainPFAMPF02204VPS9coord: 138..241
e-value: 1.8E-29
score: 102.0
IPR003123VPS9 domainPROSITEPS51205VPS9coord: 102..246
score: 42.656742
NoneNo IPR availableGENE3D1.10.246.120coord: 17..97
e-value: 9.2E-29
score: 101.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 407..444
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 300..333
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 404..453
NoneNo IPR availablePANTHERPTHR23101:SF110BNAC09G47180D PROTEINcoord: 1..410
IPR041545RABX5, catalytic core helical domainPFAMPF18151DUF5601coord: 27..91
e-value: 7.6E-17
score: 61.3
IPR037191VPS9 domain superfamilyGENE3D1.20.1050.80VPS9 domaincoord: 99..265
e-value: 7.5E-66
score: 223.0
IPR037191VPS9 domain superfamilySUPERFAMILY109993VPS9 domaincoord: 11..257
IPR045046Vacuolar protein sorting-associated protein 9-likePANTHERPTHR23101RAB GDP/GTP EXCHANGE FACTORcoord: 1..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018587.1MS018587.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016192 vesicle-mediated transport
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity