MS018463 (gene) Bitter gourd (TR) v1

Overview
NameMS018463
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionEndoglucanase
Locationscaffold898: 319454 .. 324304 (+)
RNA-Seq ExpressionMS018463
SyntenyMS018463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCGTGGTTTTATATTGGCTTTGCTCGCCGGCGTGCTCTGTTTCCAAGCCGTCGCCGTCGCCGGTGACGTCTTCAACTACGGCGATGCTGTGGATTTGAGTTTCTTGTTTCTGGAGGCGCAGAGGTCCGGTAAACTTCCGGCAGACCACCGCGTCAAGTGGCGGGGGGATTCTGGCCTCAAGGACGGCCTTGCCCAAGGAGTAAGTTTTCCCTCACTAAAATTGGTTGAATAATGAAAATTATTGTTAATACATAATTAAATTTATAATAATCTATCTGCTTATTGGATTAACGGGTTATTGAACAATTATTTACAATAATATTTTACTATAGTTCTCAATGACTAATTAGGTTATAAAATTTATGTCAAGTTGGTATACTTTATGATAGCTTTGAGAATTTTGTTTGAGCTCCAAAGTTGTAATTTTAATGACATTTTTGAACTTTAGTTTATTTGTTTGAATGGAGTGAATTTAACAATATAGTATAAACTAAATAAAAATATATCCAACAAACGGAGCCTAAGTTTTTTTTTTTTTTTTGAGTTTTAAACGCATGTTTGTTTTTAAGACAACAACATAATAAATAGACATAATAATACGTAAATGTAGATAAACTAATTTTAGAATTTAATGAAAATCACCTATTATTTAATGAAAATATAAATTTTAAGATATGGAAGGGAAATATCAATGATATTACCCGTTTGAACTTCAAAAGTAGATTAGAAACTTTCTCGAACATTTGTTATCATTTCATATGGTTTTAAGTGTGTTTAATTTAATGTTTTCTTAAAATTTCCATTTTTTATAGCTTTGAAATCCCTATTACATATATAAAATAATTATCATACAATTTGGTGGGTAACCATTTTGTTTTAAGTTTTATATTTTTTAAAATTAAACTCAGAAACACTACCGTTCATATGTGTTATTTGTTTTACTTTAAATATTGTTTTATTTTTTAAATTTAAGTAATAATTCAAATGTATTAAAAAAAATTACAATACCTTGATTTTTTAAAACAAAAAATATCATTAACTATAAAGTTATATTCATATAGTGAATTTCAAATTTTTAATATATATCTACCGACCATCCCACTACAGATTGAAACGGCCAATTATATTATATCTCTTTAGACTTTTTGTATTTATCTTTCATTACTTCTCACAAATTTAAGGGTAGTCAGTCGGCAAGATATAACATGTACCTCAACATTTTTATTTAATTATCTGTTGAATATTTGACTCATTATCGATAAGGATTAATTTTGTACAAGCTACTATTAAATATTCGATACTTGAGAACGAAAAATGCAAAAAAATTATCAAATAAACTATCAACTTTTCAAGCAAGCATTTTCCTAGTTATCCACTGGTGAAGTCATTATACAAAATCTAAAATATTTTGCAATAAATTGAACAATGGCAGGTGAATTTAGTGGGAGGGTACTACGACGCAGGAGACCACGTGAAGTTCGGGCTTCCAATGGCGTTCGTCGCCACCATACTTTCATGGGGAGCCATCGATTTCAACAAGGAAATTACAGATCTGAACCAAATGGACAATACTCTCAACGCCATTAAATGGGCCGCAGATTACTTCATCAAGGCCCACCCTCAGCGGAATGTGCTCTGGGGCCAGGTCGGCGACGGCGCCTCCGATCACTTCTGCTGGGAGCGCCCCGAAGACATGACCACTCCCAGAACCGCCTTCAAAATCGACGAGTCTCATCCCGGCTCCGACCTCGCCGGCGAAACCGCCGCCGCCCTCGCCGCCGCCTCCATCGCCTTCAAGACTTACAACTCTTCGTACTCCGATCTCCTCCTCGTTCACGCCAAGGAGGTAACAAAAAATTCAAATTCACACATATTTATTCTTTGAACGCGATCATTATAAGGAAGTTATTCGCTAATTAATATATGATTTTTTATAAAAAAATTTGGTGGCAGCTGTTCACATTTGCAGACACATTCAGAGGTTTATACGACGATTCCATTCCCTGCGCTTCGGAGTTCTACACTTCATCGGGATATTGGGTAACAAACTTCGATCCTAAGAAAATAAAAATATAGTTATTTTCTATAATTATTTGTTTTAATTTGTGGGAAATTGAATTCAGGACGAACTGCTGTGGGCGGCGGCGTGGCTGTTCAGAGCAACCGGAGACGAGTACTACTTGAAGTACACGGTGGACAAAGCGGTGTCGTTTGGGGGAACTGGATGGGCGATGAAGGAGTTCTCTTGGGATAATAAATATGCCGGCGTTCAAGTTCTTCTCACCAAGGTGTGTGATTTTATTATATTATATTATATTAATTAATTCATGGAATAATTTATTTAATTATTGTGCGAAATTTGATTTTGCAGGTTTTGTTGGAGGGACGCGGTGGGGCTTATACGACGACGTTGAAGCAATATCAGGCCAAAGCCGATTATTTCGCTTGCGCTTGTCTTCAGAAGAACGATGGATTTAACATCAACAAAACTCCTGGTAAGTTTAAGCTAAGATCACGGTTTAATTTGTTGGGATTGGGATTGGGATTGGGATTTTTAATTGGGTTTTTATGATTTAAAAATCTAATTAGTTCCTATTCTATAGTTTAGTCGTGAGACTCGTGACTCACAAATTGTTATCAATTTAGATATGTGACTTTTTTTTTTTTCATTTTGATTTACGTGGCAATTTGGTAGATTGCGTTGGTTTTGTATTAAAATGGAGTAAAAATAGGAGAAAAACTAGTTAATTTTTTGGTAGGTAAAAAATGATCTAGAGATAATTTTTTAGATTAATAACTAATTTAAACCTATATTTGATTAAAAAATCTATCGGTTCTAATACTACTAAATATAAGTGTCACTTTTATTTTATTGTTATGGTAATTATTTATTTAATAAAGGGAGCATATTTCAGCATAATCTGAGTTTGGTCCTATCCTACATTGTACTAAAAAAAAAATTTTTTTCCTTTTTTTTTGAATGTTAGCTATCTTTTAAGGTTTAGAATCAAATACAAAGATATATCTAAAAAAATAGTAAGTTTTTAGGTGGGTGAAATATTTTTCACTCAAACTTAGACTTTAATATAGTTAATTAACATAGTTTCTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAATCTTATATGATAACGTCAAATATGCACAGCAAATGGTTGTAAGTTGGACTTCAATTTTTTTTTTTTTTATGAAAATCGTAGGATGCAAATTGAACTAAGAGACTAGAATTATGGAACCAAAGTTCTGATCACCGTTTTTGATCAATTTCTGGAGTAGAAAACGAAACTAATTTTCCGATTTGATTTTCCCGGGAAAAAATCAAAACAGGGGGATTGATGTACGTGCGGGAATGGAACAATATGCAATACGCCTCCGCCGCCGCATTCCTCATCGCCGTCTACTCCGTCTACCTCTCCGAAGCCAACGCCAAGCTCATCTGCCCCGACGGCGTCTTCCAACCGCATGATCTCCTCAACTTCGCCCAATCGCAGGCCGATTACATCCTCGGCAACAATCCAAACTCCATCAGCTATTTGGTCGGCTACGGATCCAAATTTCCGCAGAAGCTTCACCACAGAGGCTCCTCGATTCCTTCGATTTTCACCTATCCCTCCACCATCGGCTGCGTTCAAGGATTCGATTCGTGGTACCATCGGCTCCAACAAAACCCTAACATTCTCCACGGCGCTCTCGTCGGCGGCCCTGACAAGAACGACAGGTTCGACGACGATCGATCGGATTATGAACAGACTGAGCCTACTCTCACTGCTTGCGCTCCTCTGATTGGACTATTTTCCAAGCTGCAGAGTGTTAATGGCAATCCTGGTAAGATGAGGACTCGAATTCGGTGTCTTATTTTTGGAAATTTCGATGTTCTTGTTTGCTGATTTCATCGTTGGAACTTGGAACAGGATCGCGCGGATATAAGCCGCCGGTAAAACGTGAAGAAGAATCACCGAAGACGAACGCCCCGGTCCCAGTGCCTGCAGGTGAGGGCAAAAAACAAAATATGTTTACAAGACATTTTTATGAACTGACTGACGTTTCTATTGGTTGAATTTCATGTGCTTCATCTTTTGACTTATAAATTACTTTCATGTAATCATAGTTTTGTTTGATTATACTCATCCAACTTCATAGAAACTTTATAATGTATTAAAACCCATCAAGTGATCATCAATATTAATTAAAAAGAAAATTACACTATATAGATATTCAAATTCAAGGTTCCCGACCTTAATATCATGTTAAAACTAGAACACTAAATCAAAAAACTTAAACTAAGAGATCACGAGCGCTACCTTCTCGAGTTTTTGCTTATTTTGGGCACATGGGTGCTTCTCTCACGGGTGCTAAGCTCATATTTCTTTTGTGTAGTCCTTCTTTATTTGTACTCGGACTCTCATGAAATTTAGTCTACATAGTGTGCTATCAATCAGACGACGAATCATAATCTCTCTAATTTTGCAGGGTGCCCGGTGGAGTTTATCCACACAATAACAAGCACATGGGCAGTGGGGAAGGAGGATTACTACAGGCACCAAGTGAAGATCAAGAACACATCCGGAAAGCCAATCAGTGGCCTAAAGCTGCGGCTGGAGAATCTCTCAGGCCCCATTTGGGGGCTCTCTCCAACCCAACAGAAAGACATTTACGAGCTTCCTCAATGGATGAAAGTGCTTCAGCCTGCCTCAGAATTCAATTTCATCTACATTCAGGGTGGCCCTCAGGCCACGGTCACTGTCTCCAGCTACCAT

mRNA sequence

ATGGCGCGTGGTTTTATATTGGCTTTGCTCGCCGGCGTGCTCTGTTTCCAAGCCGTCGCCGTCGCCGGTGACGTCTTCAACTACGGCGATGCTGTGGATTTGAGTTTCTTGTTTCTGGAGGCGCAGAGGTCCGGTAAACTTCCGGCAGACCACCGCGTCAAGTGGCGGGGGGATTCTGGCCTCAAGGACGGCCTTGCCCAAGGAGTGAATTTAGTGGGAGGGTACTACGACGCAGGAGACCACGTGAAGTTCGGGCTTCCAATGGCGTTCGTCGCCACCATACTTTCATGGGGAGCCATCGATTTCAACAAGGAAATTACAGATCTGAACCAAATGGACAATACTCTCAACGCCATTAAATGGGCCGCAGATTACTTCATCAAGGCCCACCCTCAGCGGAATGTGCTCTGGGGCCAGGTCGGCGACGGCGCCTCCGATCACTTCTGCTGGGAGCGCCCCGAAGACATGACCACTCCCAGAACCGCCTTCAAAATCGACGAGTCTCATCCCGGCTCCGACCTCGCCGGCGAAACCGCCGCCGCCCTCGCCGCCGCCTCCATCGCCTTCAAGACTTACAACTCTTCGTACTCCGATCTCCTCCTCGTTCACGCCAAGGAGCTGTTCACATTTGCAGACACATTCAGAGGTTTATACGACGATTCCATTCCCTGCGCTTCGGAGTTCTACACTTCATCGGGATATTGGGACGAACTGCTGTGGGCGGCGGCGTGGCTGTTCAGAGCAACCGGAGACGAGTACTACTTGAAGTACACGGTGGACAAAGCGGTGTCGTTTGGGGGAACTGGATGGGCGATGAAGGAGTTCTCTTGGGATAATAAATATGCCGGCGTTCAAGTTCTTCTCACCAAGGTTTTGTTGGAGGGACGCGGTGGGGCTTATACGACGACGTTGAAGCAATATCAGGCCAAAGCCGATTATTTCGCTTGCGCTTGTCTTCAGAAGAACGATGGATTTAACATCAACAAAACTCCTGGGGGATTGATGTACGTGCGGGAATGGAACAATATGCAATACGCCTCCGCCGCCGCATTCCTCATCGCCGTCTACTCCGTCTACCTCTCCGAAGCCAACGCCAAGCTCATCTGCCCCGACGGCGTCTTCCAACCGCATGATCTCCTCAACTTCGCCCAATCGCAGGCCGATTACATCCTCGGCAACAATCCAAACTCCATCAGCTATTTGGTCGGCTACGGATCCAAATTTCCGCAGAAGCTTCACCACAGAGGCTCCTCGATTCCTTCGATTTTCACCTATCCCTCCACCATCGGCTGCGTTCAAGGATTCGATTCGTGGTACCATCGGCTCCAACAAAACCCTAACATTCTCCACGGCGCTCTCGTCGGCGGCCCTGACAAGAACGACAGGTTCGACGACGATCGATCGGATTATGAACAGACTGAGCCTACTCTCACTGCTTGCGCTCCTCTGATTGGACTATTTTCCAAGCTGCAGAGTGTTAATGGCAATCCTGGATCGCGCGGATATAAGCCGCCGGTAAAACGTGAAGAAGAATCACCGAAGACGAACGCCCCGGTCCCAGTGCCTGCAGGGTGCCCGGTGGAGTTTATCCACACAATAACAAGCACATGGGCAGTGGGGAAGGAGGATTACTACAGGCACCAAGTGAAGATCAAGAACACATCCGGAAAGCCAATCAGTGGCCTAAAGCTGCGGCTGGAGAATCTCTCAGGCCCCATTTGGGGGCTCTCTCCAACCCAACAGAAAGACATTTACGAGCTTCCTCAATGGATGAAAGTGCTTCAGCCTGCCTCAGAATTCAATTTCATCTACATTCAGGGTGGCCCTCAGGCCACGGTCACTGTCTCCAGCTACCAT

Coding sequence (CDS)

ATGGCGCGTGGTTTTATATTGGCTTTGCTCGCCGGCGTGCTCTGTTTCCAAGCCGTCGCCGTCGCCGGTGACGTCTTCAACTACGGCGATGCTGTGGATTTGAGTTTCTTGTTTCTGGAGGCGCAGAGGTCCGGTAAACTTCCGGCAGACCACCGCGTCAAGTGGCGGGGGGATTCTGGCCTCAAGGACGGCCTTGCCCAAGGAGTGAATTTAGTGGGAGGGTACTACGACGCAGGAGACCACGTGAAGTTCGGGCTTCCAATGGCGTTCGTCGCCACCATACTTTCATGGGGAGCCATCGATTTCAACAAGGAAATTACAGATCTGAACCAAATGGACAATACTCTCAACGCCATTAAATGGGCCGCAGATTACTTCATCAAGGCCCACCCTCAGCGGAATGTGCTCTGGGGCCAGGTCGGCGACGGCGCCTCCGATCACTTCTGCTGGGAGCGCCCCGAAGACATGACCACTCCCAGAACCGCCTTCAAAATCGACGAGTCTCATCCCGGCTCCGACCTCGCCGGCGAAACCGCCGCCGCCCTCGCCGCCGCCTCCATCGCCTTCAAGACTTACAACTCTTCGTACTCCGATCTCCTCCTCGTTCACGCCAAGGAGCTGTTCACATTTGCAGACACATTCAGAGGTTTATACGACGATTCCATTCCCTGCGCTTCGGAGTTCTACACTTCATCGGGATATTGGGACGAACTGCTGTGGGCGGCGGCGTGGCTGTTCAGAGCAACCGGAGACGAGTACTACTTGAAGTACACGGTGGACAAAGCGGTGTCGTTTGGGGGAACTGGATGGGCGATGAAGGAGTTCTCTTGGGATAATAAATATGCCGGCGTTCAAGTTCTTCTCACCAAGGTTTTGTTGGAGGGACGCGGTGGGGCTTATACGACGACGTTGAAGCAATATCAGGCCAAAGCCGATTATTTCGCTTGCGCTTGTCTTCAGAAGAACGATGGATTTAACATCAACAAAACTCCTGGGGGATTGATGTACGTGCGGGAATGGAACAATATGCAATACGCCTCCGCCGCCGCATTCCTCATCGCCGTCTACTCCGTCTACCTCTCCGAAGCCAACGCCAAGCTCATCTGCCCCGACGGCGTCTTCCAACCGCATGATCTCCTCAACTTCGCCCAATCGCAGGCCGATTACATCCTCGGCAACAATCCAAACTCCATCAGCTATTTGGTCGGCTACGGATCCAAATTTCCGCAGAAGCTTCACCACAGAGGCTCCTCGATTCCTTCGATTTTCACCTATCCCTCCACCATCGGCTGCGTTCAAGGATTCGATTCGTGGTACCATCGGCTCCAACAAAACCCTAACATTCTCCACGGCGCTCTCGTCGGCGGCCCTGACAAGAACGACAGGTTCGACGACGATCGATCGGATTATGAACAGACTGAGCCTACTCTCACTGCTTGCGCTCCTCTGATTGGACTATTTTCCAAGCTGCAGAGTGTTAATGGCAATCCTGGATCGCGCGGATATAAGCCGCCGGTAAAACGTGAAGAAGAATCACCGAAGACGAACGCCCCGGTCCCAGTGCCTGCAGGGTGCCCGGTGGAGTTTATCCACACAATAACAAGCACATGGGCAGTGGGGAAGGAGGATTACTACAGGCACCAAGTGAAGATCAAGAACACATCCGGAAAGCCAATCAGTGGCCTAAAGCTGCGGCTGGAGAATCTCTCAGGCCCCATTTGGGGGCTCTCTCCAACCCAACAGAAAGACATTTACGAGCTTCCTCAATGGATGAAAGTGCTTCAGCCTGCCTCAGAATTCAATTTCATCTACATTCAGGGTGGCCCTCAGGCCACGGTCACTGTCTCCAGCTACCAT

Protein sequence

MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLWAAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEFNFIYIQGGPQATVTVSSYH
Homology
BLAST of MS018463 vs. NCBI nr
Match: XP_022155052.1 (endoglucanase 5 [Momordica charantia])

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 618/619 (99.84%), Postives = 619/619 (100.00%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG
Sbjct: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK
Sbjct: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA
Sbjct: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFY+SSGYWDELLW
Sbjct: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYSSSGYWDELLW 240

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY
Sbjct: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
           TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL
Sbjct: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP
Sbjct: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC
Sbjct: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480

Query: 481 APLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVG 540
           APLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVG
Sbjct: 481 APLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVG 540

Query: 541 KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF 600
           KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF
Sbjct: 541 KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF 600

Query: 601 NFIYIQGGPQATVTVSSYH 620
           NFIYIQGGPQATVTVSSYH
Sbjct: 601 NFIYIQGGPQATVTVSSYH 619

BLAST of MS018463 vs. NCBI nr
Match: XP_023548134.1 (endoglucanase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 542/622 (87.14%), Postives = 571/622 (91.80%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           M RG++LAL+A VLCF+A A AG+ FNYGDAVDLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 1   MPRGYLLALVAAVLCFEAAATAGE-FNYGDAVDLSFLYLEAQRSGKLPADRRVKWRGDSG 60

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDF KEITDLN MD  L AIK
Sbjct: 61  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFKKEITDLNHMDKALKAIK 120

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           W  DY IKAHP+RNVLW QVGDGASDHFCW+RPEDM+TPRTA+K+DESHPGSDLAGETAA
Sbjct: 121 WGTDYLIKAHPERNVLWAQVGDGASDHFCWQRPEDMSTPRTAYKLDESHPGSDLAGETAA 180

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCA++FY+SSGYWDELLW
Sbjct: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCAADFYSSSGYWDELLW 240

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR  AY
Sbjct: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRAAAY 300

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFL+AVYSVYL
Sbjct: 301 ESTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLMAVYSVYL 360

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S+ANAKL CPDGVF+P +LLNFAQSQADYILG NPNSISYL+GYGSKFPQKLHHRGSSI 
Sbjct: 361 SDANAKLTCPDGVFEPKELLNFAQSQADYILGKNPNSISYLIGYGSKFPQKLHHRGSSIA 420

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIF+ PS IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRFDDDRS+YEQTEPTLTAC
Sbjct: 421 SIFSDPSPIGCVQGFDFWYHRPQGNPNVLHGALVGGPDKNDRFDDDRSEYEQTEPTLTAC 480

Query: 481 APLIGLFSKLQ-SVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APL+GLFSKLQ SVNG   PGSRG  PPVK EE+SP  NAPVPV  G PVEFIHTITSTW
Sbjct: 481 APLLGLFSKLQSSVNGYRIPGSRGNPPPVKPEEKSPNANAPVPV--GAPVEFIHTITSTW 540

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V KE YYRHQVKIKNTSGKPIS LKLRLENLSGPIWGLSPTQQK IYELP W+++LQP 
Sbjct: 541 MVSKESYYRHQVKIKNTSGKPISDLKLRLENLSGPIWGLSPTQQKGIYELPPWLRLLQPG 600

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VTVSSYH
Sbjct: 601 SECVFIYIQEGPQAKVTVSSYH 619

BLAST of MS018463 vs. NCBI nr
Match: XP_022991484.1 (endoglucanase 5 [Cucurbita maxima])

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 571/622 (91.80%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           M RG++LAL+A VLCF+  A AGD FNYGDAVDLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 2   MPRGYLLALVAAVLCFE--AAAGD-FNYGDAVDLSFLYLEAQRSGKLPADRRVKWRGDSG 61

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDF KEITDLN MDN L AIK
Sbjct: 62  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFKKEITDLNHMDNALKAIK 121

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           W  DY IKAHP+RNVLW QVGDGASDHFCW+RPEDM+TPRTA+K+DESHPGSDLAGETAA
Sbjct: 122 WGTDYLIKAHPERNVLWAQVGDGASDHFCWQRPEDMSTPRTAYKLDESHPGSDLAGETAA 181

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFK+YNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCA++FY+SSGYWDELLW
Sbjct: 182 ALAAASIAFKSYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCAADFYSSSGYWDELLW 241

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR  AY
Sbjct: 242 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRAAAY 301

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFL+AVYSVYL
Sbjct: 302 ESTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLMAVYSVYL 361

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S+ANAKL CPDGVF+P +LLNFAQSQADYILG NPNSISYL+GYGSKFPQKLHHRGSSI 
Sbjct: 362 SDANAKLTCPDGVFEPKELLNFAQSQADYILGKNPNSISYLIGYGSKFPQKLHHRGSSID 421

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT PS IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRFDDDRS+YEQTEPTLTAC
Sbjct: 422 SIFTDPSPIGCVQGFDFWYHRPQGNPNVLHGALVGGPDKNDRFDDDRSEYEQTEPTLTAC 481

Query: 481 APLIGLFSKLQ-SVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APL+GLFSKLQ SVNG   PGSRG  PPVK E+ESP  NAPVPV  G PVEFIHTITSTW
Sbjct: 482 APLLGLFSKLQSSVNGYRIPGSRGNPPPVKPEKESPNANAPVPV--GAPVEFIHTITSTW 541

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V K+ YYRHQVKIKNTSGKPIS LKLRLENLSGP+WGLSPTQQK IYELP W+++LQP 
Sbjct: 542 MVSKDSYYRHQVKIKNTSGKPISDLKLRLENLSGPVWGLSPTQQKGIYELPPWLRLLQPG 601

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VTVSSYH
Sbjct: 602 SECVFIYIQEGPQAKVTVSSYH 618

BLAST of MS018463 vs. NCBI nr
Match: XP_022953304.1 (endoglucanase 5 [Cucurbita moschata])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 570/622 (91.64%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           M RG++LAL+A VLCF+  A AGD FNYGDAVDLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 2   MPRGYLLALVAAVLCFE--AAAGD-FNYGDAVDLSFLYLEAQRSGKLPADRRVKWRGDSG 61

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDF KEITDLN MD  L AIK
Sbjct: 62  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFKKEITDLNHMDKALKAIK 121

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           W  DY IKAHP+RNVLW QVGDGASDHFCW+RPEDM+TPRTA+K+DESHPGSDLAGETAA
Sbjct: 122 WGTDYLIKAHPERNVLWAQVGDGASDHFCWQRPEDMSTPRTAYKLDESHPGSDLAGETAA 181

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFK+YNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCA++FY+SSGYWDELLW
Sbjct: 182 ALAAASIAFKSYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCAADFYSSSGYWDELLW 241

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR  AY
Sbjct: 242 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRAAAY 301

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFL+AVYSVYL
Sbjct: 302 GSTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLMAVYSVYL 361

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S+ANAKL CPDGVF+P +LLNFAQSQADYILG NPNSISYL+GYGSKFPQKLHHRGSSI 
Sbjct: 362 SDANAKLTCPDGVFEPKELLNFAQSQADYILGKNPNSISYLIGYGSKFPQKLHHRGSSIA 421

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT PS IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRFDDDRS+YEQTEPTLTAC
Sbjct: 422 SIFTDPSPIGCVQGFDFWYHRPQGNPNVLHGALVGGPDKNDRFDDDRSEYEQTEPTLTAC 481

Query: 481 APLIGLFSKLQ-SVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APL+GLFSKLQ SVNG   PGSRG  PPVK E+ESP  NAPVPV  G PVEFIHTITSTW
Sbjct: 482 APLLGLFSKLQSSVNGYRIPGSRGNPPPVKPEKESPNANAPVPV--GAPVEFIHTITSTW 541

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V KE YYRHQVKIKNTSGKPIS LKLRLENLSGPIWGLSPTQQ+ IYELP W+++LQP 
Sbjct: 542 MVSKESYYRHQVKIKNTSGKPISDLKLRLENLSGPIWGLSPTQQEGIYELPPWLRLLQPG 601

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VTVSSYH
Sbjct: 602 SECVFIYIQEGPQAKVTVSSYH 618

BLAST of MS018463 vs. NCBI nr
Match: KAG7014275.1 (Endoglucanase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 569/622 (91.48%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           M RG++LAL+A VLCF   A AGD FNYGDAVDLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 2   MPRGYLLALVAAVLCFD--AAAGD-FNYGDAVDLSFLYLEAQRSGKLPADRRVKWRGDSG 61

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDF KEITDLN MD  L AIK
Sbjct: 62  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFKKEITDLNNMDKALKAIK 121

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           W  DY IKAHP+RNVLW QVGDGASDHFCW+RPEDM+TPRTA+K+DESHPGSDLAGETAA
Sbjct: 122 WGTDYLIKAHPERNVLWAQVGDGASDHFCWQRPEDMSTPRTAYKLDESHPGSDLAGETAA 181

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFK+YNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCA++FY+SSGYWDELLW
Sbjct: 182 ALAAASIAFKSYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCAADFYSSSGYWDELLW 241

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR  AY
Sbjct: 242 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRAAAY 301

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFL+AVYSVYL
Sbjct: 302 GSTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLMAVYSVYL 361

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S+ANAKL CPDGVF+P +LLNFAQSQADYILG NPNSISYL+GYGSKFPQKLHHRGSSI 
Sbjct: 362 SDANAKLTCPDGVFEPKELLNFAQSQADYILGKNPNSISYLIGYGSKFPQKLHHRGSSIA 421

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT PS IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRFDDDRS+YEQTEPTLTAC
Sbjct: 422 SIFTDPSPIGCVQGFDFWYHRPQGNPNVLHGALVGGPDKNDRFDDDRSEYEQTEPTLTAC 481

Query: 481 APLIGLFSKLQ-SVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APL+GLFSKLQ SV+G   PGSRG  PPVK E+ESP  NAPVPV  G PVEFIHTITSTW
Sbjct: 482 APLLGLFSKLQSSVDGYRIPGSRGNPPPVKPEKESPNANAPVPV--GAPVEFIHTITSTW 541

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V KE YYRHQVKIKNTSGKPIS LKLRLENLSGPIWGLSPTQQK IYELP W+++LQP 
Sbjct: 542 MVSKESYYRHQVKIKNTSGKPISDLKLRLENLSGPIWGLSPTQQKGIYELPPWLRLLQPG 601

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VTVSSYH
Sbjct: 602 SECVFIYIQEGPQAKVTVSSYH 618

BLAST of MS018463 vs. ExPASy Swiss-Prot
Match: Q9M995 (Endoglucanase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g48930 PE=1 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 8.5e-252
Identity = 417/620 (67.26%), Postives = 492/620 (79.35%), Query Frame = 0

Query: 5   FILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDG 64
           F ++LL  VL   A A A + +NYG A+D +FLF EAQRSGKLPA  RVKWRG SGLKDG
Sbjct: 9   FGVSLLLSVLLAAATA-AAEYYNYGSALDKTFLFFEAQRSGKLPAAQRVKWRGPSGLKDG 68

Query: 65  LAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAAD 124
           LAQGV+L GGYYDAGDHVKFGLPMAF  T+LSW A+D  KE++  NQM  TL +I+W  D
Sbjct: 69  LAQGVSLEGGYYDAGDHVKFGLPMAFAVTMLSWAAVDNRKELSSSNQMQQTLWSIRWGTD 128

Query: 125 YFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAA 184
           YFIKAHPQ NVLWGQVGDG SDH+CWERPEDMTT RTA+K+D  HPGSDLAGETAAALAA
Sbjct: 129 YFIKAHPQPNVLWGQVGDGESDHYCWERPEDMTTSRTAYKLDPYHPGSDLAGETAAALAA 188

Query: 185 ASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLWAAAW 244
           AS+AFK +NSSYS LLL HAKELF+FAD +RGLY +SIP A  FY SSGY DELLWAAAW
Sbjct: 189 ASLAFKPFNSSYSALLLSHAKELFSFADKYRGLYTNSIPNAKAFYMSSGYSDELLWAAAW 248

Query: 245 LFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTL 304
           L RATGD+YYLKY +D +   GGTGW +KEFSWDNKYAGVQ+LL+K+LLEG+GG YT+TL
Sbjct: 249 LHRATGDQYYLKYAMDNSGYMGGTGWGVKEFSWDNKYAGVQILLSKILLEGKGGIYTSTL 308

Query: 305 KQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEAN 364
           KQYQ KADYFACACL+KN G+NI  TPGGLMYVREWNN+QYASAAA+L+AVYS YLS AN
Sbjct: 309 KQYQTKADYFACACLKKNGGYNIQTTPGGLMYVREWNNLQYASAAAYLLAVYSDYLSAAN 368

Query: 365 AKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFT 424
           AKL CPDG+ QP  LL+FA+SQADYILG N   +SY+VGYG K+P ++HHRGSSIPSIF 
Sbjct: 369 AKLNCPDGLVQPQGLLDFARSQADYILGKNRQGMSYVVGYGPKYPIRVHHRGSSIPSIFA 428

Query: 425 YPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLI 484
             S++ CVQGFDSWY R Q +PN+++GALVGGPD+ND + DDRS+YEQ+EPTL+  APL+
Sbjct: 429 QRSSVSCVQGFDSWYRRSQGDPNVIYGALVGGPDENDNYSDDRSNYEQSEPTLSGTAPLV 488

Query: 485 GLFSKLQSVN-GNPGSRGYKP--PVKREEESPKTNAPV--PVPAGCPVEFIHTITSTWAV 544
           GLF+KL   + G+ G   YKP    K    S K       P  +G  +EF+H+ITS W  
Sbjct: 489 GLFAKLYGGSLGSYGGGSYKPYETTKPAASSYKATPTTYSPKQSGAQIEFLHSITSNWIA 548

Query: 545 GKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASE 604
           G   YYRH+V IKN S KPIS LKL++E+LSGPIWGL+PT QK  Y+LPQW K L+    
Sbjct: 549 GNTRYYRHKVIIKNNSQKPISDLKLKIEDLSGPIWGLNPTGQKYTYQLPQWQKTLRAGQA 608

Query: 605 FNFIYIQGGPQATVTVSSYH 620
           ++F+Y+QGGPQA V+V SY+
Sbjct: 609 YDFVYVQGGPQAKVSVLSYN 627

BLAST of MS018463 vs. ExPASy Swiss-Prot
Match: A2XYW8 (Endoglucanase 13 OS=Oryza sativa subsp. indica OX=39946 GN=GLU6 PE=3 SV=2)

HSP 1 Score: 759.2 bits (1959), Expect = 3.6e-218
Identity = 354/609 (58.13%), Postives = 445/609 (73.07%), Query Frame = 0

Query: 13  VLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGLAQGVNLV 72
           +L   A +V    F+Y  A D   LF EAQRSGKLP D  V+WRGDS L DG +QGV+LV
Sbjct: 24  LLLAAAASVEASAFDYAGAFDKCLLFFEAQRSGKLPDDRLVRWRGDSALTDGFSQGVDLV 83

Query: 73  GGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADYFIKAHPQ 132
           GGYYD+GDHVKFGLPMA+  T+LSWG ++F KE+ D N++   L+AI+W  +YF+KAH Q
Sbjct: 84  GGYYDSGDHVKFGLPMAYAVTMLSWGVVEFEKEMVDGNKLHRVLDAIRWGTNYFVKAHTQ 143

Query: 133 RNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAASIAFKTY 192
            N LW QVGDG SDH CWER EDM+TPRTAFKID ++PGS++AGETAAALAAA+ AFK Y
Sbjct: 144 HNALWVQVGDGDSDHLCWERAEDMSTPRTAFKIDINNPGSEVAGETAAALAAAAKAFKPY 203

Query: 193 NSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELLWAAAWLFRATGD 252
           +  YSDLLL+H+K+LFTFADTFRG YDDS+  A +FY S SGY DELLWAAAWL+ ATGD
Sbjct: 204 DRMYSDLLLLHSKQLFTFADTFRGKYDDSLQSAKKFYPSASGYQDELLWAAAWLYEATGD 263

Query: 253 EYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGA--YTTTLKQYQA 312
           E YL+Y    A +FGGTGWA+ EFSWDNKYAG+QVLL+KVL E  G A  Y  TLKQYQA
Sbjct: 264 EQYLRYVSQNAEAFGGTGWAVTEFSWDNKYAGLQVLLSKVLFEQGGSAAGYADTLKQYQA 323

Query: 313 KADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEANAKLIC 372
           KA++F CACLQKN+G N+  TPGGLMYV +W+NMQY S++AFL+ VY+ YL+E+   L C
Sbjct: 324 KAEFFLCACLQKNNGHNVKMTPGGLMYVSDWSNMQYVSSSAFLLTVYADYLAESRGTLRC 383

Query: 373 PDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFTYPSTI 432
           PDG  +P ++L FA+SQ DY+LG NP  +SY+VGYGS +P  +HHRG+SIPSI+   +T+
Sbjct: 384 PDGEVKPAEILLFARSQVDYVLGKNPKGMSYMVGYGSYYPTHVHHRGASIPSIYAMNATV 443

Query: 433 GCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLIGLFSK 492
           GC++GFD +Y+    +PN+LHGALVGGPD ND +DDDR +Y+  EPTL   AP+ G+F++
Sbjct: 444 GCMEGFDKYYNSKNADPNVLHGALVGGPDANDAYDDDRCNYQHAEPTLAGNAPMSGVFAR 503

Query: 493 LQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVGKEDYYRHQV 552
           L +           P     E +P  NAP P   G P+EF+HT+T+TW     DYYRH V
Sbjct: 504 LAA----------SPADNTPEYTPAPNAPSPSNGGSPLEFVHTVTNTWKANGVDYYRHVV 563

Query: 553 KIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEFNFIYIQGGP 612
             KNT G  I+ LKL+++ LSG I+G+S T  KD+YE P WM  L   ++   +YIQGGP
Sbjct: 564 TAKNTCGHAITYLKLQIKELSGEIYGVSRTNAKDMYEFPSWMTRLDAGAQLTIVYIQGGP 622

Query: 613 QATVTVSSY 619
            A + V  Y
Sbjct: 624 AAKIAVVEY 622

BLAST of MS018463 vs. ExPASy Swiss-Prot
Match: Q0J930 (Endoglucanase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU6 PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 8.0e-218
Identity = 353/609 (57.96%), Postives = 444/609 (72.91%), Query Frame = 0

Query: 13  VLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGLAQGVNLV 72
           +L   A +V    F+Y  A D   LF EAQRSGKLP D  V+WRGDS L DG +QGV+LV
Sbjct: 24  LLLAAAASVEASAFDYAGAFDKCLLFFEAQRSGKLPDDRLVRWRGDSALTDGFSQGVDLV 83

Query: 73  GGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADYFIKAHPQ 132
           GGYYD+GDHVKFGLPMA+  T+LSWG ++F KE+ D N++   L+AI+W  +YF+KAH Q
Sbjct: 84  GGYYDSGDHVKFGLPMAYAVTMLSWGVVEFEKEMVDGNKLHRVLDAIRWGTNYFVKAHTQ 143

Query: 133 RNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAASIAFKTY 192
            N LW QVGDG SDH CWER EDM+TPRTAFKID ++PGS++AGETAAALAAA+ AFK Y
Sbjct: 144 HNALWVQVGDGDSDHLCWERAEDMSTPRTAFKIDINNPGSEVAGETAAALAAAAKAFKPY 203

Query: 193 NSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELLWAAAWLFRATGD 252
           +  YSDLLL+H+K+LFTFADTFRG YDDS+  A +FY S SGY DELLWAAAWL+ ATGD
Sbjct: 204 DRMYSDLLLLHSKQLFTFADTFRGKYDDSLQSAKKFYPSASGYQDELLWAAAWLYEATGD 263

Query: 253 EYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGA--YTTTLKQYQA 312
           E YL+Y    A +FGGTGWA+ EFSWDNKYAG+QVLL+KVL E  G A  Y  TLKQYQA
Sbjct: 264 EQYLRYVSQNAEAFGGTGWAVTEFSWDNKYAGLQVLLSKVLFEQGGSAAGYADTLKQYQA 323

Query: 313 KADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEANAKLIC 372
           KA++F CACLQKN+G N+  TPGGLMYV +W+NMQY S++AFL+ VY+ YL+E+   L C
Sbjct: 324 KAEFFLCACLQKNNGHNVKMTPGGLMYVSDWSNMQYVSSSAFLLTVYADYLAESRGTLRC 383

Query: 373 PDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFTYPSTI 432
           PDG  +P ++L FA+SQ DY+LG NP  +SY+VGYGS +P  +HHRG+SIPSI+   +T+
Sbjct: 384 PDGEVKPAEILRFARSQVDYVLGKNPKGMSYMVGYGSYYPTHVHHRGASIPSIYAMNATV 443

Query: 433 GCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLIGLFSK 492
           GC++ FD +Y+    +PN+LHGALVGGPD ND +DDDR +Y+  EPTL   AP+ G+F++
Sbjct: 444 GCMESFDKYYNSKNADPNVLHGALVGGPDANDAYDDDRCNYQHAEPTLAGNAPMSGVFAR 503

Query: 493 LQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVGKEDYYRHQV 552
           L +           P     E +P  NAP P   G P+EF+HT+T+TW     DYYRH V
Sbjct: 504 LAA----------SPADNTPEYTPAPNAPSPSNGGSPLEFVHTVTNTWKANGVDYYRHVV 563

Query: 553 KIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEFNFIYIQGGP 612
             KNT G  I+ LKL+++ LSG I+G+S T  KD+YE P WM  L   ++   +YIQGGP
Sbjct: 564 TAKNTCGHAITYLKLQIKELSGEIYGVSRTNAKDMYEFPSWMTRLDAGAQLTIVYIQGGP 622

Query: 613 QATVTVSSY 619
            A + V  Y
Sbjct: 624 AAKIAVVEY 622

BLAST of MS018463 vs. ExPASy Swiss-Prot
Match: Q42059 (Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2)

HSP 1 Score: 625.2 bits (1611), Expect = 8.1e-178
Identity = 311/622 (50.00%), Postives = 410/622 (65.92%), Query Frame = 0

Query: 6   ILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGL 65
           + ALL  +LCF  VA +G   +YG A+  S LF EAQRSG LP + RV WR  SGL DG 
Sbjct: 7   VAALLLLLLCF-PVAFSG--HDYGQALSKSLLFFEAQRSGVLPRNQRVTWRSHSGLTDGK 66

Query: 66  AQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADY 125
           + GVNLVGGYYDAGD+VKFGLPMAF  T+++W  I++  ++    ++ N+++AIKW  DY
Sbjct: 67  SSGVNLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGNQLQANGELGNSIDAIKWGTDY 126

Query: 126 FIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAA 185
           FIKAHP+ NVL+G+VGDG +DH+CW+RPE+MTT R A++ID S+PGSDLAGETAAA+AAA
Sbjct: 127 FIKAHPEPNVLYGEVGDGNTDHYCWQRPEEMTTDRKAYRIDPSNPGSDLAGETAAAMAAA 186

Query: 186 SIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELLWAAAW 245
           SI F+  N  YS LLL HA +LF FAD +RG YD SI  A ++Y S SGY DELLWAAAW
Sbjct: 187 SIVFRRSNPVYSRLLLTHAYQLFDFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAW 246

Query: 246 LFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTL 305
           L++A+ +++YL Y      + GGTGW+M EF WD KYAGVQ L+ K L++G+ G +    
Sbjct: 247 LYQASNNQFYLDYLGRNGDAMGGTGWSMTEFGWDVKYAGVQTLVAKFLMQGKAGRHAPVF 306

Query: 306 KQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEAN 365
           ++YQ KAD F C+ L K+   NI KTPGGL++ + WNNMQ+ ++A+FL  VYS YL+ + 
Sbjct: 307 RKYQEKADSFMCSLLGKSSR-NIQKTPGGLIFRQRWNNMQFVTSASFLTTVYSDYLTSSR 366

Query: 366 AKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFT 425
           + L C  G   P  LL+FA+SQ DYILG+NP + SY+VGYG+ FPQ++HHRGSSI S+  
Sbjct: 367 SNLRCAAGNVAPSQLLSFAKSQVDYILGDNPRATSYMVGYGNNFPQRVHHRGSSIVSVKV 426

Query: 426 YPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLI 485
             + + C  G+ +W+ R   +PN+L GA+VGGPD  D F D R +YEQTEP     APL+
Sbjct: 427 DRTFVTCRGGYATWFSRKGSDPNLLTGAIVGGPDAYDNFADRRDNYEQTEPATYNNAPLL 486

Query: 486 GLFSKLQSVNGNPGSRGYK--------PPVKREEESPKTNAPVPVPAGCPVEFIHTITST 545
           G+ ++L S     G  GY         P V+R     +     PV A  PV  +  ITS+
Sbjct: 487 GVLARLSS-----GHSGYSQFLPVVPAPVVRRPMPIRRPKVTTPVRASGPVAIVQKITSS 546

Query: 546 WAVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQP 605
           W      YYR+   + N S +P+  L L ++NL GPIWGLS  +  + + LP WM  L  
Sbjct: 547 WVSKGRTYYRYSTTVINKSSRPLKSLNLSIKNLYGPIWGLS--RSGNSFGLPSWMHSLPS 606

Query: 606 ASEFNFIYIQGGPQATVTVSSY 619
                F+YI     A V VSSY
Sbjct: 607 GKSLEFVYIHSTTPANVAVSSY 617

BLAST of MS018463 vs. ExPASy Swiss-Prot
Match: Q8L7I0 (Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 5.6e-171
Identity = 300/618 (48.54%), Postives = 397/618 (64.24%), Query Frame = 0

Query: 13  VLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGLAQGVNLV 72
           VL    V +A    +Y  A+  S LF EAQRSG LP + RV WR  SGL DG + GV+LV
Sbjct: 12  VLLLGLVQLAISGHDYKQALSKSILFFEAQRSGHLPPNQRVSWRSHSGLYDGKSSGVDLV 71

Query: 73  GGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADYFIKAHPQ 132
           GGYYDAGD+VKFGLPMAF  T + W  I++  ++    ++ + ++A+KW  DYFIKAHP+
Sbjct: 72  GGYYDAGDNVKFGLPMAFTVTTMCWSIIEYGGQLESNGELGHAIDAVKWGTDYFIKAHPE 131

Query: 133 RNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAASIAFKTY 192
            NVL+G+VGDG SDH+CW+RPE+MTT R A+KID ++PGSDLAGETAAA+AAASI F+  
Sbjct: 132 PNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNNPGSDLAGETAAAMAAASIVFRRS 191

Query: 193 NSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELLWAAAWLFRATGD 252
           + SYS  LL HA +LF FAD +RG YD SI  A ++Y S SGY DELLWAAAWL++AT D
Sbjct: 192 DPSYSAELLRHAHQLFEFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAWLYQATND 251

Query: 253 EYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTLKQYQAKA 312
           +YYL Y      S GGTGW+M EF WD KYAGVQ L+ KVL++G+GG +T   ++YQ KA
Sbjct: 252 KYYLDYLGKNGDSMGGTGWSMTEFGWDVKYAGVQTLVAKVLMQGKGGEHTAVFERYQQKA 311

Query: 313 DYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEANAKLICPD 372
           + F C+ L K+   NI KTPGGL++ + WNNMQ+ ++A+FL  VYS YLS +   L+C  
Sbjct: 312 EQFMCSLLGKSTK-NIKKTPGGLIFRQSWNNMQFVTSASFLATVYSDYLSYSKRDLLCSQ 371

Query: 373 GVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFTYPSTIGC 432
           G   P  LL F++SQ DYILG+NP + SY+VGYG  +P+++HHRGSSI S       + C
Sbjct: 372 GNISPSQLLEFSKSQVDYILGDNPRATSYMVGYGENYPRQVHHRGSSIVSFNVDQKFVTC 431

Query: 433 VQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLIGLFSKLQ 492
             G+ +W+ R   +PN+L GALVGGPD  D F D R +YEQTEP     APL+G+ ++L 
Sbjct: 432 RGGYATWFSRKGSDPNVLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPLLGVLARL- 491

Query: 493 SVNGNPGSRGYKPPVKREEESPKTNAPVPVP-----------AGCPVEFIHTITSTWAVG 552
            ++G+ G     P V     SP    P PVP           +  P+     +T++W   
Sbjct: 492 -ISGSTGFDQLLPGVS-PTPSPVIIKPAPVPQRKPTKPPAASSPSPITISQKMTNSWKNE 551

Query: 553 KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF 612
            + YYR+   + N S K +  LK+ +  L GPIWG++ T     +  P WM+ L      
Sbjct: 552 GKVYYRYSTILTNRSTKTLKILKISITKLYGPIWGVTKTGNS--FSFPSWMQSLPSGKSM 611

Query: 613 NFIYIQGGPQATVTVSSY 619
            F+YI     A V VS+Y
Sbjct: 612 EFVYIHSASPADVLVSNY 623

BLAST of MS018463 vs. ExPASy TrEMBL
Match: A0A6J1DNC1 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111022190 PE=3 SV=1)

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 618/619 (99.84%), Postives = 619/619 (100.00%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG
Sbjct: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK
Sbjct: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA
Sbjct: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFY+SSGYWDELLW
Sbjct: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYSSSGYWDELLW 240

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY
Sbjct: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
           TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL
Sbjct: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP
Sbjct: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC
Sbjct: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480

Query: 481 APLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVG 540
           APLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVG
Sbjct: 481 APLIGLFSKLQSVNGNPGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTWAVG 540

Query: 541 KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF 600
           KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF
Sbjct: 541 KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF 600

Query: 601 NFIYIQGGPQATVTVSSYH 620
           NFIYIQGGPQATVTVSSYH
Sbjct: 601 NFIYIQGGPQATVTVSSYH 619

BLAST of MS018463 vs. ExPASy TrEMBL
Match: A0A6J1JUZ6 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111488085 PE=3 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 571/622 (91.80%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           M RG++LAL+A VLCF+  A AGD FNYGDAVDLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 2   MPRGYLLALVAAVLCFE--AAAGD-FNYGDAVDLSFLYLEAQRSGKLPADRRVKWRGDSG 61

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDF KEITDLN MDN L AIK
Sbjct: 62  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFKKEITDLNHMDNALKAIK 121

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           W  DY IKAHP+RNVLW QVGDGASDHFCW+RPEDM+TPRTA+K+DESHPGSDLAGETAA
Sbjct: 122 WGTDYLIKAHPERNVLWAQVGDGASDHFCWQRPEDMSTPRTAYKLDESHPGSDLAGETAA 181

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFK+YNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCA++FY+SSGYWDELLW
Sbjct: 182 ALAAASIAFKSYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCAADFYSSSGYWDELLW 241

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR  AY
Sbjct: 242 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRAAAY 301

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFL+AVYSVYL
Sbjct: 302 ESTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLMAVYSVYL 361

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S+ANAKL CPDGVF+P +LLNFAQSQADYILG NPNSISYL+GYGSKFPQKLHHRGSSI 
Sbjct: 362 SDANAKLTCPDGVFEPKELLNFAQSQADYILGKNPNSISYLIGYGSKFPQKLHHRGSSID 421

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT PS IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRFDDDRS+YEQTEPTLTAC
Sbjct: 422 SIFTDPSPIGCVQGFDFWYHRPQGNPNVLHGALVGGPDKNDRFDDDRSEYEQTEPTLTAC 481

Query: 481 APLIGLFSKLQ-SVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APL+GLFSKLQ SVNG   PGSRG  PPVK E+ESP  NAPVPV  G PVEFIHTITSTW
Sbjct: 482 APLLGLFSKLQSSVNGYRIPGSRGNPPPVKPEKESPNANAPVPV--GAPVEFIHTITSTW 541

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V K+ YYRHQVKIKNTSGKPIS LKLRLENLSGP+WGLSPTQQK IYELP W+++LQP 
Sbjct: 542 MVSKDSYYRHQVKIKNTSGKPISDLKLRLENLSGPVWGLSPTQQKGIYELPPWLRLLQPG 601

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VTVSSYH
Sbjct: 602 SECVFIYIQEGPQAKVTVSSYH 618

BLAST of MS018463 vs. ExPASy TrEMBL
Match: A0A6J1GP97 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111455891 PE=3 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 541/622 (86.98%), Postives = 570/622 (91.64%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           M RG++LAL+A VLCF+  A AGD FNYGDAVDLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 2   MPRGYLLALVAAVLCFE--AAAGD-FNYGDAVDLSFLYLEAQRSGKLPADRRVKWRGDSG 61

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDF KEITDLN MD  L AIK
Sbjct: 62  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFKKEITDLNHMDKALKAIK 121

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           W  DY IKAHP+RNVLW QVGDGASDHFCW+RPEDM+TPRTA+K+DESHPGSDLAGETAA
Sbjct: 122 WGTDYLIKAHPERNVLWAQVGDGASDHFCWQRPEDMSTPRTAYKLDESHPGSDLAGETAA 181

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFK+YNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCA++FY+SSGYWDELLW
Sbjct: 182 ALAAASIAFKSYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCAADFYSSSGYWDELLW 241

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR  AY
Sbjct: 242 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRAAAY 301

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFL+AVYSVYL
Sbjct: 302 GSTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLMAVYSVYL 361

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S+ANAKL CPDGVF+P +LLNFAQSQADYILG NPNSISYL+GYGSKFPQKLHHRGSSI 
Sbjct: 362 SDANAKLTCPDGVFEPKELLNFAQSQADYILGKNPNSISYLIGYGSKFPQKLHHRGSSIA 421

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT PS IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRFDDDRS+YEQTEPTLTAC
Sbjct: 422 SIFTDPSPIGCVQGFDFWYHRPQGNPNVLHGALVGGPDKNDRFDDDRSEYEQTEPTLTAC 481

Query: 481 APLIGLFSKLQ-SVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APL+GLFSKLQ SVNG   PGSRG  PPVK E+ESP  NAPVPV  G PVEFIHTITSTW
Sbjct: 482 APLLGLFSKLQSSVNGYRIPGSRGNPPPVKPEKESPNANAPVPV--GAPVEFIHTITSTW 541

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V KE YYRHQVKIKNTSGKPIS LKLRLENLSGPIWGLSPTQQ+ IYELP W+++LQP 
Sbjct: 542 MVSKESYYRHQVKIKNTSGKPISDLKLRLENLSGPIWGLSPTQQEGIYELPPWLRLLQPG 601

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VTVSSYH
Sbjct: 602 SECVFIYIQEGPQAKVTVSSYH 618

BLAST of MS018463 vs. ExPASy TrEMBL
Match: A0A5A7URX3 (Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00120 PE=3 SV=1)

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 534/622 (85.85%), Postives = 567/622 (91.16%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           MA+GF++AL+A VLCF+A A AG+ FNYGDA+DLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 1   MAQGFLVALVAAVLCFEAAAAAGE-FNYGDALDLSFLYLEAQRSGKLPADRRVKWRGDSG 60

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDFNKEIT+ NQMDNTL AIK
Sbjct: 61  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFNKEITNANQMDNTLKAIK 120

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           WA DYF+KAH  RNVLWGQVGDG+SDHFCW+RPEDMTTPRTAFKIDESHPGSDLAGETAA
Sbjct: 121 WATDYFLKAHTSRNVLWGQVGDGSSDHFCWQRPEDMTTPRTAFKIDESHPGSDLAGETAA 180

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFKTYNS+YS+LLLVHAKELFTFADTFRGLYDDSIPCAS FYTSSGYWDELLW
Sbjct: 181 ALAAASIAFKTYNSAYSNLLLVHAKELFTFADTFRGLYDDSIPCASGFYTSSGYWDELLW 240

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR G Y
Sbjct: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRSGGY 300

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDG+NINKTPGGL+Y  EWNNMQYASAAAFL+AVYS YL
Sbjct: 301 ESTLKQYQAKADYFACACLQKNDGYNINKTPGGLLYAHEWNNMQYASAAAFLMAVYSDYL 360

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S ANAKLICPDGVF+P +LLNFAQSQADYILG NPNS+SYL+GYG KFPQKLHHRGSSI 
Sbjct: 361 SAANAKLICPDGVFEPKELLNFAQSQADYILGKNPNSLSYLIGYGPKFPQKLHHRGSSIA 420

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT P+ IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRF DDRS+YEQTEPTLTA 
Sbjct: 421 SIFTDPAPIGCVQGFDYWYHRPQGNPNVLHGALVGGPDKNDRFGDDRSEYEQTEPTLTAS 480

Query: 481 APLIGLFSKL-QSVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APLIGLFSKL  SVNG+  PGSRG++PPVKRE ESP  N  VPV AG PVEFIHTITSTW
Sbjct: 481 APLIGLFSKLHSSVNGHQIPGSRGHQPPVKRENESPDAN--VPVAAGSPVEFIHTITSTW 540

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V KE YYRHQVKIKNTSGK I  LKL LENL+GPIWGLSPTQQK IYELP+W+ VLQP 
Sbjct: 541 TVNKESYYRHQVKIKNTSGKSIKNLKLHLENLTGPIWGLSPTQQKGIYELPEWLTVLQPG 600

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VT+SSYH
Sbjct: 601 SECVFIYIQEGPQAKVTISSYH 619

BLAST of MS018463 vs. ExPASy TrEMBL
Match: A0A1S3CEZ8 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103499972 PE=3 SV=1)

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 534/622 (85.85%), Postives = 567/622 (91.16%), Query Frame = 0

Query: 1   MARGFILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 60
           MA+GF++AL+A VLCF+A A AG+ FNYGDA+DLSFL+LEAQRSGKLPAD RVKWRGDSG
Sbjct: 1   MAQGFLVALVAAVLCFEAAAAAGE-FNYGDALDLSFLYLEAQRSGKLPADRRVKWRGDSG 60

Query: 61  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 120
           LKDGLAQGVNLVGGYYDAGD+VKFGLPMAFV TILSWGAIDFNKEIT+ NQMDNTL AIK
Sbjct: 61  LKDGLAQGVNLVGGYYDAGDNVKFGLPMAFVTTILSWGAIDFNKEITNANQMDNTLKAIK 120

Query: 121 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 180
           WA DYF+KAH  RNVLWGQVGDG+SDHFCW+RPEDMTTPRTAFKIDESHPGSDLAGETAA
Sbjct: 121 WATDYFLKAHTSRNVLWGQVGDGSSDHFCWQRPEDMTTPRTAFKIDESHPGSDLAGETAA 180

Query: 181 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLW 240
           ALAAASIAFKTYNS+YS+LLLVHAKELFTFADTFRGLYDDSIPCAS FYTSSGYWDELLW
Sbjct: 181 ALAAASIAFKTYNSAYSNLLLVHAKELFTFADTFRGLYDDSIPCASGFYTSSGYWDELLW 240

Query: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAY 300
           AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGR G Y
Sbjct: 241 AAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRSGGY 300

Query: 301 TTTLKQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYL 360
            +TLKQYQAKADYFACACLQKNDG+NINKTPGGL+Y  EWNNMQYASAAAFL+AVYS YL
Sbjct: 301 ESTLKQYQAKADYFACACLQKNDGYNINKTPGGLLYAHEWNNMQYASAAAFLMAVYSDYL 360

Query: 361 SEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIP 420
           S ANAKLICPDGVF+P +LLNFAQSQADYILG NPNS+SYL+GYG KFPQKLHHRGSSI 
Sbjct: 361 SAANAKLICPDGVFEPKELLNFAQSQADYILGKNPNSLSYLIGYGPKFPQKLHHRGSSIA 420

Query: 421 SIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTAC 480
           SIFT P+ IGCVQGFD WYHR Q NPN+LHGALVGGPDKNDRF DDRS+YEQTEPTLTA 
Sbjct: 421 SIFTDPAPIGCVQGFDYWYHRPQGNPNVLHGALVGGPDKNDRFGDDRSEYEQTEPTLTAS 480

Query: 481 APLIGLFSKL-QSVNGN--PGSRGYKPPVKREEESPKTNAPVPVPAGCPVEFIHTITSTW 540
           APLIGLFSKL  SVNG+  PGSRG++PPVKRE ESP  N  VPV AG PVEFIHTITSTW
Sbjct: 481 APLIGLFSKLHSSVNGHQIPGSRGHQPPVKRENESPDAN--VPVAAGSPVEFIHTITSTW 540

Query: 541 AVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPA 600
            V KE YYRHQVKIKNTSGK I  LKL LENL+GPIWGLSPTQQK IYELP+W+ VLQP 
Sbjct: 541 TVNKESYYRHQVKIKNTSGKSIKNLKLHLENLTGPIWGLSPTQQKGIYELPEWLTVLQPG 600

Query: 601 SEFNFIYIQGGPQATVTVSSYH 620
           SE  FIYIQ GPQA VT+SSYH
Sbjct: 601 SECVFIYIQEGPQAKVTISSYH 619

BLAST of MS018463 vs. TAIR 10
Match: AT1G48930.1 (glycosyl hydrolase 9C1 )

HSP 1 Score: 870.9 bits (2249), Expect = 6.0e-253
Identity = 417/620 (67.26%), Postives = 492/620 (79.35%), Query Frame = 0

Query: 5   FILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDG 64
           F ++LL  VL   A A A + +NYG A+D +FLF EAQRSGKLPA  RVKWRG SGLKDG
Sbjct: 9   FGVSLLLSVLLAAATA-AAEYYNYGSALDKTFLFFEAQRSGKLPAAQRVKWRGPSGLKDG 68

Query: 65  LAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAAD 124
           LAQGV+L GGYYDAGDHVKFGLPMAF  T+LSW A+D  KE++  NQM  TL +I+W  D
Sbjct: 69  LAQGVSLEGGYYDAGDHVKFGLPMAFAVTMLSWAAVDNRKELSSSNQMQQTLWSIRWGTD 128

Query: 125 YFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAA 184
           YFIKAHPQ NVLWGQVGDG SDH+CWERPEDMTT RTA+K+D  HPGSDLAGETAAALAA
Sbjct: 129 YFIKAHPQPNVLWGQVGDGESDHYCWERPEDMTTSRTAYKLDPYHPGSDLAGETAAALAA 188

Query: 185 ASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTSSGYWDELLWAAAW 244
           AS+AFK +NSSYS LLL HAKELF+FAD +RGLY +SIP A  FY SSGY DELLWAAAW
Sbjct: 189 ASLAFKPFNSSYSALLLSHAKELFSFADKYRGLYTNSIPNAKAFYMSSGYSDELLWAAAW 248

Query: 245 LFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTL 304
           L RATGD+YYLKY +D +   GGTGW +KEFSWDNKYAGVQ+LL+K+LLEG+GG YT+TL
Sbjct: 249 LHRATGDQYYLKYAMDNSGYMGGTGWGVKEFSWDNKYAGVQILLSKILLEGKGGIYTSTL 308

Query: 305 KQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEAN 364
           KQYQ KADYFACACL+KN G+NI  TPGGLMYVREWNN+QYASAAA+L+AVYS YLS AN
Sbjct: 309 KQYQTKADYFACACLKKNGGYNIQTTPGGLMYVREWNNLQYASAAAYLLAVYSDYLSAAN 368

Query: 365 AKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFT 424
           AKL CPDG+ QP  LL+FA+SQADYILG N   +SY+VGYG K+P ++HHRGSSIPSIF 
Sbjct: 369 AKLNCPDGLVQPQGLLDFARSQADYILGKNRQGMSYVVGYGPKYPIRVHHRGSSIPSIFA 428

Query: 425 YPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLI 484
             S++ CVQGFDSWY R Q +PN+++GALVGGPD+ND + DDRS+YEQ+EPTL+  APL+
Sbjct: 429 QRSSVSCVQGFDSWYRRSQGDPNVIYGALVGGPDENDNYSDDRSNYEQSEPTLSGTAPLV 488

Query: 485 GLFSKLQSVN-GNPGSRGYKP--PVKREEESPKTNAPV--PVPAGCPVEFIHTITSTWAV 544
           GLF+KL   + G+ G   YKP    K    S K       P  +G  +EF+H+ITS W  
Sbjct: 489 GLFAKLYGGSLGSYGGGSYKPYETTKPAASSYKATPTTYSPKQSGAQIEFLHSITSNWIA 548

Query: 545 GKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASE 604
           G   YYRH+V IKN S KPIS LKL++E+LSGPIWGL+PT QK  Y+LPQW K L+    
Sbjct: 549 GNTRYYRHKVIIKNNSQKPISDLKLKIEDLSGPIWGLNPTGQKYTYQLPQWQKTLRAGQA 608

Query: 605 FNFIYIQGGPQATVTVSSYH 620
           ++F+Y+QGGPQA V+V SY+
Sbjct: 609 YDFVYVQGGPQAKVSVLSYN 627

BLAST of MS018463 vs. TAIR 10
Match: AT1G64390.1 (glycosyl hydrolase 9C2 )

HSP 1 Score: 625.2 bits (1611), Expect = 5.7e-179
Identity = 311/622 (50.00%), Postives = 410/622 (65.92%), Query Frame = 0

Query: 6   ILALLAGVLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGL 65
           + ALL  +LCF  VA +G   +YG A+  S LF EAQRSG LP + RV WR  SGL DG 
Sbjct: 7   VAALLLLLLCF-PVAFSG--HDYGQALSKSLLFFEAQRSGVLPRNQRVTWRSHSGLTDGK 66

Query: 66  AQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADY 125
           + GVNLVGGYYDAGD+VKFGLPMAF  T+++W  I++  ++    ++ N+++AIKW  DY
Sbjct: 67  SSGVNLVGGYYDAGDNVKFGLPMAFTVTMMAWSVIEYGNQLQANGELGNSIDAIKWGTDY 126

Query: 126 FIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAA 185
           FIKAHP+ NVL+G+VGDG +DH+CW+RPE+MTT R A++ID S+PGSDLAGETAAA+AAA
Sbjct: 127 FIKAHPEPNVLYGEVGDGNTDHYCWQRPEEMTTDRKAYRIDPSNPGSDLAGETAAAMAAA 186

Query: 186 SIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELLWAAAW 245
           SI F+  N  YS LLL HA +LF FAD +RG YD SI  A ++Y S SGY DELLWAAAW
Sbjct: 187 SIVFRRSNPVYSRLLLTHAYQLFDFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAW 246

Query: 246 LFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTL 305
           L++A+ +++YL Y      + GGTGW+M EF WD KYAGVQ L+ K L++G+ G +    
Sbjct: 247 LYQASNNQFYLDYLGRNGDAMGGTGWSMTEFGWDVKYAGVQTLVAKFLMQGKAGRHAPVF 306

Query: 306 KQYQAKADYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEAN 365
           ++YQ KAD F C+ L K+   NI KTPGGL++ + WNNMQ+ ++A+FL  VYS YL+ + 
Sbjct: 307 RKYQEKADSFMCSLLGKSSR-NIQKTPGGLIFRQRWNNMQFVTSASFLTTVYSDYLTSSR 366

Query: 366 AKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFT 425
           + L C  G   P  LL+FA+SQ DYILG+NP + SY+VGYG+ FPQ++HHRGSSI S+  
Sbjct: 367 SNLRCAAGNVAPSQLLSFAKSQVDYILGDNPRATSYMVGYGNNFPQRVHHRGSSIVSVKV 426

Query: 426 YPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLI 485
             + + C  G+ +W+ R   +PN+L GA+VGGPD  D F D R +YEQTEP     APL+
Sbjct: 427 DRTFVTCRGGYATWFSRKGSDPNLLTGAIVGGPDAYDNFADRRDNYEQTEPATYNNAPLL 486

Query: 486 GLFSKLQSVNGNPGSRGYK--------PPVKREEESPKTNAPVPVPAGCPVEFIHTITST 545
           G+ ++L S     G  GY         P V+R     +     PV A  PV  +  ITS+
Sbjct: 487 GVLARLSS-----GHSGYSQFLPVVPAPVVRRPMPIRRPKVTTPVRASGPVAIVQKITSS 546

Query: 546 WAVGKEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQP 605
           W      YYR+   + N S +P+  L L ++NL GPIWGLS  +  + + LP WM  L  
Sbjct: 547 WVSKGRTYYRYSTTVINKSSRPLKSLNLSIKNLYGPIWGLS--RSGNSFGLPSWMHSLPS 606

Query: 606 ASEFNFIYIQGGPQATVTVSSY 619
                F+YI     A V VSSY
Sbjct: 607 GKSLEFVYIHSTTPANVAVSSY 617

BLAST of MS018463 vs. TAIR 10
Match: AT4G11050.1 (glycosyl hydrolase 9C3 )

HSP 1 Score: 602.4 bits (1552), Expect = 4.0e-172
Identity = 300/618 (48.54%), Postives = 397/618 (64.24%), Query Frame = 0

Query: 13  VLCFQAVAVAGDVFNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGLAQGVNLV 72
           VL    V +A    +Y  A+  S LF EAQRSG LP + RV WR  SGL DG + GV+LV
Sbjct: 12  VLLLGLVQLAISGHDYKQALSKSILFFEAQRSGHLPPNQRVSWRSHSGLYDGKSSGVDLV 71

Query: 73  GGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADYFIKAHPQ 132
           GGYYDAGD+VKFGLPMAF  T + W  I++  ++    ++ + ++A+KW  DYFIKAHP+
Sbjct: 72  GGYYDAGDNVKFGLPMAFTVTTMCWSIIEYGGQLESNGELGHAIDAVKWGTDYFIKAHPE 131

Query: 133 RNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAASIAFKTY 192
            NVL+G+VGDG SDH+CW+RPE+MTT R A+KID ++PGSDLAGETAAA+AAASI F+  
Sbjct: 132 PNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNNPGSDLAGETAAAMAAASIVFRRS 191

Query: 193 NSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELLWAAAWLFRATGD 252
           + SYS  LL HA +LF FAD +RG YD SI  A ++Y S SGY DELLWAAAWL++AT D
Sbjct: 192 DPSYSAELLRHAHQLFEFADKYRGKYDSSITVAQKYYRSVSGYNDELLWAAAWLYQATND 251

Query: 253 EYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTLKQYQAKA 312
           +YYL Y      S GGTGW+M EF WD KYAGVQ L+ KVL++G+GG +T   ++YQ KA
Sbjct: 252 KYYLDYLGKNGDSMGGTGWSMTEFGWDVKYAGVQTLVAKVLMQGKGGEHTAVFERYQQKA 311

Query: 313 DYFACACLQKNDGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEANAKLICPD 372
           + F C+ L K+   NI KTPGGL++ + WNNMQ+ ++A+FL  VYS YLS +   L+C  
Sbjct: 312 EQFMCSLLGKSTK-NIKKTPGGLIFRQSWNNMQFVTSASFLATVYSDYLSYSKRDLLCSQ 371

Query: 373 GVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFTYPSTIGC 432
           G   P  LL F++SQ DYILG+NP + SY+VGYG  +P+++HHRGSSI S       + C
Sbjct: 372 GNISPSQLLEFSKSQVDYILGDNPRATSYMVGYGENYPRQVHHRGSSIVSFNVDQKFVTC 431

Query: 433 VQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLIGLFSKLQ 492
             G+ +W+ R   +PN+L GALVGGPD  D F D R +YEQTEP     APL+G+ ++L 
Sbjct: 432 RGGYATWFSRKGSDPNVLTGALVGGPDAYDNFADQRDNYEQTEPATYNNAPLLGVLARL- 491

Query: 493 SVNGNPGSRGYKPPVKREEESPKTNAPVPVP-----------AGCPVEFIHTITSTWAVG 552
            ++G+ G     P V     SP    P PVP           +  P+     +T++W   
Sbjct: 492 -ISGSTGFDQLLPGVS-PTPSPVIIKPAPVPQRKPTKPPAASSPSPITISQKMTNSWKNE 551

Query: 553 KEDYYRHQVKIKNTSGKPISGLKLRLENLSGPIWGLSPTQQKDIYELPQWMKVLQPASEF 612
            + YYR+   + N S K +  LK+ +  L GPIWG++ T     +  P WM+ L      
Sbjct: 552 GKVYYRYSTILTNRSTKTLKILKISITKLYGPIWGVTKTGNS--FSFPSWMQSLPSGKSM 611

Query: 613 NFIYIQGGPQATVTVSSY 619
            F+YI     A V VS+Y
Sbjct: 612 EFVYIHSASPADVLVSNY 623

BLAST of MS018463 vs. TAIR 10
Match: AT2G32990.1 (glycosyl hydrolase 9B8 )

HSP 1 Score: 576.2 bits (1484), Expect = 3.1e-164
Identity = 272/492 (55.28%), Postives = 352/492 (71.54%), Query Frame = 0

Query: 5   FILALLAGVL--CFQAVAVAGDV--FNYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSG 64
           F+L LL  V       V+   DV  F+YG+A+  S L+ EAQRSG+LP + RV WR  SG
Sbjct: 13  FLLLLLITVFSAALDGVSSETDVGGFDYGEALSKSLLYFEAQRSGRLPYNQRVTWRDHSG 72

Query: 65  LKDGLAQGVNLVGGYYDAGDHVKFGLPMAFVATILSWGAIDFNKEITDLNQMDNTLNAIK 124
           L DGL QGV+LVGGY+DAGDHVKFGLPMAF  T+LSW  I++   +    ++ + L AIK
Sbjct: 73  LTDGLEQGVDLVGGYHDAGDHVKFGLPMAFTVTMLSWSVIEYGDSLASTGELSHALEAIK 132

Query: 125 WAADYFIKAHPQRNVLWGQVGDGASDHFCWERPEDMTTPRTAFKIDESHPGSDLAGETAA 184
           W  DYFIKAH   NVLW +VGDG +DH+CW+RPEDMTT R AFKIDE++PGSD+AGETAA
Sbjct: 133 WGTDYFIKAHTSPNVLWAEVGDGDTDHYCWQRPEDMTTSRRAFKIDENNPGSDIAGETAA 192

Query: 185 ALAAASIAFKTYNSSYSDLLLVHAKELFTFADTFRGLYDDSIPCASEFYTS-SGYWDELL 244
           A+AAASI F++ N  YS LLL HA++LF F D +RG YD+S+     +Y S SGY DELL
Sbjct: 193 AMAAASIVFRSTNPHYSHLLLHHAQQLFEFGDKYRGKYDESLKVVKSYYASVSGYMDELL 252

Query: 245 WAAAWLFRATGDEYYLKYTVDKAVSFGGTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGA 304
           W A WL+RAT +E+Y+ Y VD A   GG  WAM EFSWD K+AGVQ+L + +L E +   
Sbjct: 253 WGATWLYRATDNEHYMSYVVDMAHQLGGLSWAMSEFSWDVKFAGVQLLASMLLKEEKHKQ 312

Query: 305 YTTTLKQYQAKADYFACACLQKN-DGFNINKTPGGLMYVREWNNMQYASAAAFLIAVYSV 364
           ++  L+QY++KAD++ C+ L KN +G N+ +TP GL+YVR+WNNMQY S A+FL+ VYS 
Sbjct: 313 HSKVLQQYKSKADHYLCSILNKNINGTNVQRTPAGLLYVRQWNNMQYVSTASFLLTVYSD 372

Query: 365 YLSEANAKLICPDGVFQPHDLLNFAQSQADYILGNNPNSISYLVGYGSKFPQKLHHRGSS 424
           +L ++N  L C +G   P ++L FA+SQ DYILG+NP   SYLVGYG K+P ++HHRG+S
Sbjct: 373 HLRKSNTDLECHEGTVTPDEMLGFAKSQIDYILGSNPMETSYLVGYGPKYPIRVHHRGAS 432

Query: 425 IPSIFTYPSTIGCVQGFDSWYHRLQQNPNILHGALVGGPDKNDRFDDDRSDYEQTEPTLT 484
           I S   +   IGC QG+D+WY R + NP++L GALVGGPD  D FDD R +Y QTE    
Sbjct: 433 IASFKEHKGFIGCTQGYDNWYGRSEPNPSVLVGALVGGPDHQDDFDDRRGNYVQTEACTY 492

Query: 485 ACAPLIGLFSKL 491
             APL+G+F++L
Sbjct: 493 NTAPLVGVFARL 504

BLAST of MS018463 vs. TAIR 10
Match: AT2G44560.1 (glycosyl hydrolase 9B11 )

HSP 1 Score: 526.6 bits (1355), Expect = 2.8e-149
Identity = 244/462 (52.81%), Postives = 325/462 (70.35%), Query Frame = 0

Query: 27  NYGDAVDLSFLFLEAQRSGKLPADHRVKWRGDSGLKDGLAQGVNLVGGYYDAGDHVKFGL 86
           NYGDA+  S L+ EAQRSGKLP++ RV WRGDS L+DG    V+L GGYYDAGD++KFG 
Sbjct: 33  NYGDALTKSLLYFEAQRSGKLPSNQRVTWRGDSALRDGSDAHVDLTGGYYDAGDNMKFGF 92

Query: 87  PMAFVATILSWGAIDFNKEITDLNQMDNTLNAIKWAADYFIKAHPQRNVLWGQVGDGASD 146
           P+AF  T+L+W  I+   ++    + +N L A+KWA D+ IKAHP+ NVL+GQVGDG SD
Sbjct: 93  PLAFFTTMLAWSNIEMATQLKAHQEQENALAALKWATDFLIKAHPEPNVLYGQVGDGNSD 152

Query: 147 HFCWERPEDMTTPRTAFKIDESHPGSDLAGETAAALAAASIAFKTYNSSYSDLLLVHAKE 206
           H CW RPEDMTTPR +F+ID  HPGSDLAGETAAA+AAASIAF   + +Y+ +L+ HAKE
Sbjct: 153 HECWMRPEDMTTPRPSFRIDAQHPGSDLAGETAAAMAAASIAFAPSDEAYAQILIGHAKE 212

Query: 207 LFTFADTFRGLYDDSIPCASEFYTSSGYWDELLWAAAWLFRATGDEYYLKYTVDKAVSFG 266
           LF FA  + G+Y +SI  A  FY SSGY DELLWAAAWL RAT D+ YL Y    +    
Sbjct: 213 LFEFAKAYPGIYQNSITNAGGFYASSGYEDELLWAAAWLHRATNDQIYLDYLTQAS---- 272

Query: 267 GTGWAMKEFSWDNKYAGVQVLLTKVLLEGRGGAYTTTLKQYQAKADYFACACLQKNDGFN 326
           GTG     FSWD+K+ G QVL+ K+ LEG+  +    + +Y++ A+ F C C QK    N
Sbjct: 273 GTGGPRTAFSWDDKFVGAQVLVAKLALEGKVES-NGKIAEYKSMAEQFICNCAQKGSN-N 332

Query: 327 INKTPGGLMYVREWNNMQYASAAAFLIAVYSVYLSEANAKLICPDGVFQPHDLLNFAQSQ 386
           + KTPGGL+Y   WNN+QY +AA F+++ YS YL++A A + CP+G  Q  DLL+ A+SQ
Sbjct: 333 VKKTPGGLLYFLPWNNLQYTTAATFVLSAYSKYLTDAKASIQCPNGALQASDLLDLARSQ 392

Query: 387 ADYILGNNPNSISYLVGYGSKFPQKLHHRGSSIPSIFTYPSTIGCVQGFDSWYHRLQQNP 446
            DYILG+NP ++SY+VG G+ +P+K HHR +SI SI    + + C +GFD+W++    NP
Sbjct: 393 VDYILGSNPQNMSYMVGVGTNYPKKPHHRAASIVSITKDKTPVTCSEGFDAWFNNPAPNP 452

Query: 447 NILHGALVGGPDKNDRFDDDRSDYEQTEPTLTACAPLIGLFS 489
           N+L GA+VGGP+ ND + D+R+DY+  EP     AP +G+ +
Sbjct: 453 NVLMGAVVGGPNDNDVYGDERTDYQHAEPAPATAAPFVGVLA 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155052.10.0e+0099.84endoglucanase 5 [Momordica charantia][more]
XP_023548134.10.0e+0087.14endoglucanase 5 [Cucurbita pepo subsp. pepo][more]
XP_022991484.10.0e+0086.98endoglucanase 5 [Cucurbita maxima][more]
XP_022953304.10.0e+0086.98endoglucanase 5 [Cucurbita moschata][more]
KAG7014275.10.0e+0086.98Endoglucanase 5, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9M9958.5e-25267.26Endoglucanase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g48930 PE=1 SV=1[more]
A2XYW83.6e-21858.13Endoglucanase 13 OS=Oryza sativa subsp. indica OX=39946 GN=GLU6 PE=3 SV=2[more]
Q0J9308.0e-21857.96Endoglucanase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU6 PE=2 SV=1[more]
Q420598.1e-17850.00Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2[more]
Q8L7I05.6e-17148.54Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DNC10.0e+0099.84Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111022190 PE=3 SV=1[more]
A0A6J1JUZ60.0e+0086.98Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111488085 PE=3 SV=1[more]
A0A6J1GP970.0e+0086.98Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111455891 PE=3 SV=1[more]
A0A5A7URX30.0e+0085.85Endoglucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00120 ... [more]
A0A1S3CEZ80.0e+0085.85Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103499972 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G48930.16.0e-25367.26glycosyl hydrolase 9C1 [more]
AT1G64390.15.7e-17950.00glycosyl hydrolase 9C2 [more]
AT4G11050.14.0e-17248.54glycosyl hydrolase 9C3 [more]
AT2G32990.13.1e-16455.28glycosyl hydrolase 9B8 [more]
AT2G44560.12.8e-14952.81glycosyl hydrolase 9B11 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019028Carbohydrate binding domain CBM49SMARTSM01063CBM49_2coord: 527..607
e-value: 4.1E-17
score: 72.9
IPR019028Carbohydrate binding domain CBM49PFAMPF09478CBM49coord: 528..606
e-value: 5.2E-22
score: 78.0
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 25..494
e-value: 1.4E-169
score: 566.7
IPR001701Glycoside hydrolase family 9PFAMPF00759Glyco_hydro_9coord: 28..486
e-value: 9.8E-142
score: 473.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 495..519
NoneNo IPR availablePANTHERPTHR22298:SF41ENDOGLUCANASE 5coord: 7..576
NoneNo IPR availablePANTHERPTHR22298ENDO-1,4-BETA-GLUCANASEcoord: 7..576
IPR018221Glycoside hydrolase family 9, His active sitePROSITEPS00592GH9_2coord: 389..415
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 26..497

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018463.1MS018463.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds