MS018410 (gene) Bitter gourd (TR) v1

Overview
NameMS018410
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionadenylate isopentenyltransferase 3, chloroplastic
Locationscaffold342: 757013 .. 758005 (+)
RNA-Seq ExpressionMS018410
SyntenyMS018410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATGGCCACCATGTTCATGAGCAAACAGCTCATCACTCAGCAACCCTCGATAAATCTTCCCCCTGGAAGACTAAACACCAACCCTAATTTCTGGAAGAAGAAGCTGCAAAAAGAGAAGGTGGTGATTGTGATCGGCGCCACCGGCACGGGCAAGTCCCGACTATCGATCGACATCGCCACCCGGTTCTCATCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGAGGGTCTCGACGTAGTAACCAACAAGGTCACCAAGCAAGAACAACAGGGAGTGCCCCATCACTTGCTCGGAACAATCAATCCCTTCTATGATTTCTCGGCGACCGACTTCTCCGACATGGCCGGGCTTTCCTTGGACGCTATCGCCGCCCGCGATCGCCTCCCGATCATCGCTGGGGGCTCTAATTCTTACATAGAAGCCTTAATCGACCCAAACTTTCAATCGAGATACGAATGCTGTTTCCTTTGGGTGGATGTGTCAATGCCTATATTGTGTTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAACGGAATGGTCGATGAGGCTGAAAAGTTCTTTGATCCTGCCAGAGATTATTCTCGTGGGATCAAAAGGGCAATCGGGGTTCCTGAATTCGACTCGTATTTTCGATACAAATCATTGCTAGACGAAGAAACTCAAGATAAACTTCTCCAAGATGCAATATCAAAAGTGAAGGACCACACATGCAGGCTAGCATTGAGACAATTAGGGAAAATCCAACGGCTCAAAAACGTCAAGGGATGGAAGATACACCAATTGGATGCAACAGAGGCCTTTACAAAGCAAGGGAAAGAATCAGAGGAAGCATGGAACAAGCTTGTGGCGGCACCGAGCATGGCGATAATCGAAAATTTTCTTCACCAAAACGTCTTCACGGTGAAAGCCCCGACGACCTTGGCGTCCCTCATCGGCATACCGAGGAAGAGAAGTGCAGCCATTGTAGCTGCAACTCCT

mRNA sequence

ATGAATATGGCCACCATGTTCATGAGCAAACAGCTCATCACTCAGCAACCCTCGATAAATCTTCCCCCTGGAAGACTAAACACCAACCCTAATTTCTGGAAGAAGAAGCTGCAAAAAGAGAAGGTGGTGATTGTGATCGGCGCCACCGGCACGGGCAAGTCCCGACTATCGATCGACATCGCCACCCGGTTCTCATCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGAGGGTCTCGACGTAGTAACCAACAAGGTCACCAAGCAAGAACAACAGGGAGTGCCCCATCACTTGCTCGGAACAATCAATCCCTTCTATGATTTCTCGGCGACCGACTTCTCCGACATGGCCGGGCTTTCCTTGGACGCTATCGCCGCCCGCGATCGCCTCCCGATCATCGCTGGGGGCTCTAATTCTTACATAGAAGCCTTAATCGACCCAAACTTTCAATCGAGATACGAATGCTGTTTCCTTTGGGTGGATGTGTCAATGCCTATATTGTGTTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAACGGAATGGTCGATGAGGCTGAAAAGTTCTTTGATCCTGCCAGAGATTATTCTCGTGGGATCAAAAGGGCAATCGGGGTTCCTGAATTCGACTCGTATTTTCGATACAAATCATTGCTAGACGAAGAAACTCAAGATAAACTTCTCCAAGATGCAATATCAAAAGTGAAGGACCACACATGCAGGCTAGCATTGAGACAATTAGGGAAAATCCAACGGCTCAAAAACGTCAAGGGATGGAAGATACACCAATTGGATGCAACAGAGGCCTTTACAAAGCAAGGGAAAGAATCAGAGGAAGCATGGAACAAGCTTGTGGCGGCACCGAGCATGGCGATAATCGAAAATTTTCTTCACCAAAACGTCTTCACGGTGAAAGCCCCGACGACCTTGGCGTCCCTCATCGGCATACCGAGGAAGAGAAGTGCAGCCATTGTAGCTGCAACTCCT

Coding sequence (CDS)

ATGAATATGGCCACCATGTTCATGAGCAAACAGCTCATCACTCAGCAACCCTCGATAAATCTTCCCCCTGGAAGACTAAACACCAACCCTAATTTCTGGAAGAAGAAGCTGCAAAAAGAGAAGGTGGTGATTGTGATCGGCGCCACCGGCACGGGCAAGTCCCGACTATCGATCGACATCGCCACCCGGTTCTCATCGGAAGTCATCAACTCCGACAAAATGCAAGTCTACGAGGGTCTCGACGTAGTAACCAACAAGGTCACCAAGCAAGAACAACAGGGAGTGCCCCATCACTTGCTCGGAACAATCAATCCCTTCTATGATTTCTCGGCGACCGACTTCTCCGACATGGCCGGGCTTTCCTTGGACGCTATCGCCGCCCGCGATCGCCTCCCGATCATCGCTGGGGGCTCTAATTCTTACATAGAAGCCTTAATCGACCCAAACTTTCAATCGAGATACGAATGCTGTTTCCTTTGGGTGGATGTGTCAATGCCTATATTGTGTTCCTTTGTATCCAAACGAGTAGACAAGATGGTAGCGAACGGAATGGTCGATGAGGCTGAAAAGTTCTTTGATCCTGCCAGAGATTATTCTCGTGGGATCAAAAGGGCAATCGGGGTTCCTGAATTCGACTCGTATTTTCGATACAAATCATTGCTAGACGAAGAAACTCAAGATAAACTTCTCCAAGATGCAATATCAAAAGTGAAGGACCACACATGCAGGCTAGCATTGAGACAATTAGGGAAAATCCAACGGCTCAAAAACGTCAAGGGATGGAAGATACACCAATTGGATGCAACAGAGGCCTTTACAAAGCAAGGGAAAGAATCAGAGGAAGCATGGAACAAGCTTGTGGCGGCACCGAGCATGGCGATAATCGAAAATTTTCTTCACCAAAACGTCTTCACGGTGAAAGCCCCGACGACCTTGGCGTCCCTCATCGGCATACCGAGGAAGAGAAGTGCAGCCATTGTAGCTGCAACTCCT

Protein sequence

MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLHQNVFTVKAPTTLASLIGIPRKRSAAIVAATP
Homology
BLAST of MS018410 vs. NCBI nr
Match: XP_022150248.1 (adenylate isopentenyltransferase 3, chloroplastic [Momordica charantia])

HSP 1 Score: 651.7 bits (1680), Expect = 3.3e-183
Identity = 328/331 (99.09%), Postives = 329/331 (99.40%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL
Sbjct: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMP+LCSFVSKRVDKMV
Sbjct: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPVLCSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH
Sbjct: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIE FLH
Sbjct: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIEKFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
           QNVFTVKAPTTLASLIGIPRKRSAAI AATP
Sbjct: 301 QNVFTVKAPTTLASLIGIPRKRSAAIAAATP 331

BLAST of MS018410 vs. NCBI nr
Match: XP_023514910.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538.9 bits (1387), Expect = 3.1e-149
Identity = 268/331 (80.97%), Postives = 300/331 (90.63%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN++TMFMS+Q ITQQP INLPP R+NTNP F  +KL+KEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNVSTMFMSQQ-ITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRF SEVINSDKMQVY+GLD++TNKVTKQEQ+GVPHHLLG I+PF DFSAT+FSDMA L
Sbjct: 61  ATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLD I  RDRLPIIAGGSNSY+EALID NFQS+YECCFLWVDVSMP+L SFVSKRVDKMV
Sbjct: 121 SLDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLL++ETQ+KLLQ+AISKVK +
Sbjct: 181 ANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKY 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGKE+EE WNKLV  PS  II+ FLH
Sbjct: 241 TCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            NV T K PT LA+L+G+PRKRSAAI AATP
Sbjct: 301 SNVITTKVPTNLATLLGLPRKRSAAIAAATP 330

BLAST of MS018410 vs. NCBI nr
Match: XP_038899604.1 (adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 538.5 bits (1386), Expect = 4.1e-149
Identity = 270/331 (81.57%), Postives = 297/331 (89.73%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           M+++TMFMSKQ ITQQP INLPPGRLNTNP F  +K QKEKVVIVIGATGTGKSRLS+DI
Sbjct: 1   MDVSTMFMSKQ-ITQQPLINLPPGRLNTNPFFNLRKPQKEKVVIVIGATGTGKSRLSVDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRF SEVINSDKMQVY GLD+VTNK+TKQEQ+GVPHHLLG INPF DFSAT+FSDMA L
Sbjct: 61  ATRFPSEVINSDKMQVYRGLDIVTNKITKQEQRGVPHHLLGIINPFSDFSATNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLDAI + DRLPIIAGGSNSYIEALID  F+S+YECCFLWVDVSM +L SFVS+RVDKMV
Sbjct: 121 SLDAITSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAEKFFDP RDYSRGIKRAIGVPEFDSYFRYK LLDEE QDKLL+++ISKVK+H
Sbjct: 181 ANGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKPLLDEEAQDKLLKESISKVKEH 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LALRQLGKIQRL+N+KGW IH+LDATEAFTKQGKE+EEAWNKLV  PS  +I+ FLH
Sbjct: 241 TCKLALRQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTVPSTTLIDQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            NV   K PT LASLIG+ RKRSAA+ AATP
Sbjct: 301 PNVVATKFPTNLASLIGVSRKRSAAMAAATP 330

BLAST of MS018410 vs. NCBI nr
Match: XP_023004596.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 537.3 bits (1383), Expect = 9.0e-149
Identity = 270/331 (81.57%), Postives = 299/331 (90.33%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN++TMFMS+Q ITQQP INLPP R+NTNP F  +KL+KEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNVSTMFMSQQ-ITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           AT+F SEVINSDKMQVY+GLD+VTNKVTKQEQ+GVPHHLLG I+PF DFSAT+FSDMA L
Sbjct: 61  ATQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLD I  RDRLPIIAGGSNSYIEALID NFQS+YECCFLWVDVSMPIL SFVSKRVDKMV
Sbjct: 121 SLDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMV+EA++FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ+KLLQ+AISKVK +
Sbjct: 181 ANGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKY 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGKE+EE WNKLV  PS  IIE FLH
Sbjct: 241 TCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            NV T   PT LASL+G+PRKRSAAI AATP
Sbjct: 301 SNVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of MS018410 vs. NCBI nr
Match: KAG7025533.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 534.6 bits (1376), Expect = 5.9e-148
Identity = 267/331 (80.66%), Postives = 298/331 (90.03%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN++TMFMS+Q ITQQP INLPP R+NTNP F  +KL+KEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNVSTMFMSQQ-ITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRF SEVINSDKMQVY+GLD++TNKVTKQEQ+GVPHHLLG I+PF DFSAT+FSDMA L
Sbjct: 61  ATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLD I  RDRLPIIAGGSNSY+EALID NFQS+YECCFLWVDVSMP+L SFVSKRVDKMV
Sbjct: 121 SLDTIFGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ+KLLQ+AISKVK +
Sbjct: 181 ANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKY 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGKE+EE WNKLV  PS  II+ FLH
Sbjct: 241 TCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            NV T K PT LA+L+ + RKRSAAI AATP
Sbjct: 301 SNVITTKVPTNLATLLDLSRKRSAAIAAATP 330

BLAST of MS018410 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-84
Identity = 156/301 (51.83%), Postives = 215/301 (71.43%), Query Frame = 0

Query: 5   TMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDIATRF 64
           +M M KQ +   P+++ PP R   N         K+KVV+++GATGTGKSRLS+DIATRF
Sbjct: 6   SMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIATRF 65

Query: 65  SSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGLSLDA 124
            +E+INSDK+QV++GLD+VTNK+T +E  GVPHHLLG + P  D +A ++  MA LS+++
Sbjct: 66  RAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLSIES 125

Query: 125 IAARDRLPIIAGGSNSYIEALIDP---NFQSRYECCFLWVDVSMPILCSFVSKRVDKMVA 184
           +  R +LPII GGSNSY+EAL+D     F+SRY+CCFLWVDV++P+L  FVS+RVDKMV 
Sbjct: 126 VLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDKMVE 185

Query: 185 NGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 244
           +GMV+E  +FFD +  DYSRGIK+AIG PEFD +FR +  L+ E +++LL   + ++K +
Sbjct: 186 SGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEIKRN 245

Query: 245 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK--ESEEAWNKLVAAPSMAIIENF 300
           T  LA RQ  KI+RL+ VK W I ++DAT  FTK+    ++  AW +LVA PS   +  F
Sbjct: 246 TFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTVSRF 305

BLAST of MS018410 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 310.1 bits (793), Expect = 3.1e-83
Identity = 157/267 (58.80%), Postives = 205/267 (76.78%), Query Frame = 0

Query: 38  QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 97
           +K+KVV V+GATGTGKSRL+ID+ATRF +E++NSDK+QVY+GLD+VTNKVT +E  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 98  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDP--NFQSRY 157
           HLLGT++  Y DF+A DF   A  ++++I  RDR+PIIAGGSNSYIEAL++   +F+ RY
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRLRY 150

Query: 158 ECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDP-ARDYSRGIKRAIGVPEFDS 217
            CCFLWVDVS P+L SFVS+RVDKMV  G+VDE  + FDP + DYS GI+RAIGVPE D 
Sbjct: 151 NCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDE 210

Query: 218 YFRYKSL-LDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAF 277
           + R +      ET ++LL+ AI K+K++TC LA RQL KIQRL     W +H++DATE F
Sbjct: 211 FLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270

Query: 278 TKQGKESEEAWNKLVAAPSMAIIENFL 300
            ++G+E++EAW+  VA PS   +E FL
Sbjct: 271 LRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of MS018410 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 143/271 (52.77%), Postives = 200/271 (73.80%), Query Frame = 0

Query: 39  KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 98
           KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y+GLDV+TNKVT +E +GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 99  LLGTI-NPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPN---FQSRY 158
           LLG   +   + +AT +S +A  ++  ++A ++LPI+AGGSNSYIEAL++ +     + Y
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 159 ECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 218
           +CCF+WVDVS+P+L SFVSKRVD+M+  G+++E  + F+P  +YS GI+RAIGVPE   Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 219 FRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWK--IHQLDATEAF 278
            R +SL+D  T+ K+L  A+  +K +T  LA RQL KIQRL   K WK  +H++DATE F
Sbjct: 213 LRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLH--KKWKMSMHRVDATEVF 272

Query: 279 TKQG-KESEEAWNKLVAAPSMAIIENFLHQN 303
            K+  +E +EAW  LVA PS  I++ F + N
Sbjct: 273 LKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of MS018410 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 135/319 (42.32%), Postives = 194/319 (60.82%), Query Frame = 0

Query: 22  PPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLD 81
           P     TN +  +++ +KEK+++++GATGTGKSRLSID+A  F  EVINSDKMQVY+GLD
Sbjct: 12  PTTTTTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLD 71

Query: 82  VVTNKVTKQEQQGVPHHLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNS 141
           + TNK++  ++ GVPHHLLG ++P   + +  DF  +AG ++  I  R +LP++ GGSNS
Sbjct: 72  ITTNKISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNS 131

Query: 142 YIEALIDPNFQS------------------RYECCFLWVDVSMPILCSFVSKRVDKMVAN 201
           +I AL+   F S                  RY+CCFLWVDVS+ +L  +++KRVD M+  
Sbjct: 132 FIHALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLEL 191

Query: 202 GMVDEAEKFFDPA-RDYSR------GIKRAIGVPEFDSYFRYKSLLDEETQDK------- 261
           GM DE  +F+ P   D+        G+++AIGVPEFD YF      D E +D        
Sbjct: 192 GMFDELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRR 251

Query: 262 -LLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAF-----TKQGKESEEA 302
              ++A+  +K++TC LA RQ+GKI RLK   GW + +LDATE+F     +  G++  E 
Sbjct: 252 GAFEEAVRAIKENTCHLAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEI 311

BLAST of MS018410 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-62
Identity = 131/300 (43.67%), Postives = 195/300 (65.00%), Query Frame = 0

Query: 34  KKKLQKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQ 93
           + + +K+KVV+++GATG GKSRLS+D+ATRF SE+INSDK+QVYEGL++ TN++T Q+++
Sbjct: 59  RSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRR 118

Query: 94  GVPHHLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALI----DP 153
           GVPHHLLG INP + + +A +F   A   +  I +R ++PIIAGGSNS++ AL+    DP
Sbjct: 119 GVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDP 178

Query: 154 NFQS------------RYECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDPAR 213
            F              RYECCF+WVDVS  +L  ++ +RVD+M+ +GM +E  +F+DP +
Sbjct: 179 KFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVK 238

Query: 214 ---DYSRGIKRAIGVPEFDSYFR----YKSLLDEETQDKLLQD-AISKVKDHTCRLALRQ 273
              +   GI++AIGVPEFD YF+     K ++  +   K   D A+  +K +T  LA RQ
Sbjct: 239 SGLETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQ 298

Query: 274 LGKIQRLKNVKGWKIHQLDATEAF------TKQGKESEEAWNKLVAAPSMAIIENFLHQN 303
           + KI+ LK+  GW+I ++DAT +F      +   K+  E W + V  PS+ I++  L QN
Sbjct: 299 VKKIEMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of MS018410 vs. ExPASy TrEMBL
Match: A0A6J1D8Y2 (adenylate isopentenyltransferase 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018461 PE=4 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.6e-183
Identity = 328/331 (99.09%), Postives = 329/331 (99.40%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL
Sbjct: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMP+LCSFVSKRVDKMV
Sbjct: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPVLCSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH
Sbjct: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIE FLH
Sbjct: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIEKFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
           QNVFTVKAPTTLASLIGIPRKRSAAI AATP
Sbjct: 301 QNVFTVKAPTTLASLIGIPRKRSAAIAAATP 331

BLAST of MS018410 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.4e-149
Identity = 270/331 (81.57%), Postives = 299/331 (90.33%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN++TMFMS+Q ITQQP INLPP R+NTNP F  +KL+KEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNVSTMFMSQQ-ITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           AT+F SEVINSDKMQVY+GLD+VTNKVTKQEQ+GVPHHLLG I+PF DFSAT+FSDMA L
Sbjct: 61  ATQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLD I  RDRLPIIAGGSNSYIEALID NFQS+YECCFLWVDVSMPIL SFVSKRVDKMV
Sbjct: 121 SLDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMV+EA++FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ+KLLQ+AISKVK +
Sbjct: 181 ANGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKY 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGKE+EE WNKLV  PS  IIE FLH
Sbjct: 241 TCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            NV T   PT LASL+G+PRKRSAAI AATP
Sbjct: 301 SNVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of MS018410 vs. ExPASy TrEMBL
Match: A0A0A0K919 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.1e-147
Identity = 267/331 (80.66%), Postives = 297/331 (89.73%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN+++MFMSK + TQQP INLPPGRL TNP    +KLQKEKVVIVIG TGTGKSRLSIDI
Sbjct: 1   MNVSSMFMSK-ITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRF SEVINSDKMQVY GLD+VTNK+TKQEQ GVPHHLLG INPF DFSA++FSDMA L
Sbjct: 61  ATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLDAI++ DRLPIIAGGSNSYIEALID NF+ RYECCFLWVDVSM +L SFVS+RVDKMV
Sbjct: 121 SLDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRY+SLLDEE Q+KLL+++I+KVK+H
Sbjct: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEH 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LALRQLGKIQRL+NVKGW IH+LDATEAFTKQGKE++EAWNK V  PS  II+ FLH
Sbjct: 241 TCKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
           QNV   K PT LASLIG+PRK+SAA+ AATP
Sbjct: 301 QNVIATKFPTNLASLIGVPRKKSAAMAAATP 330

BLAST of MS018410 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 9.1e-147
Identity = 266/331 (80.36%), Postives = 296/331 (89.43%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN++TMFMS+Q ITQQP INLPP R+NTNP F  +KL+KEKVVIVIGATGTGKSRLSIDI
Sbjct: 1   MNVSTMFMSQQ-ITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRF SEVINSDKMQVY+GLD+VTNKVTKQEQ+GVPHHLLG I+PF DFSAT+FSDMA L
Sbjct: 61  ATRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SL  I  RDRLPIIAGGSNSY+EALID NFQS+YECCFLWVDVSMP+L SFVSKRVDKMV
Sbjct: 121 SLVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+ETQ+KLLQ+AISKVK +
Sbjct: 181 ANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKY 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LA RQ+GKIQRL+N+KGWKIH+LDATEAF KQGKE+EE WNKLV  PS  II+ FLH
Sbjct: 241 TCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            NV T K PT LA+L+ + R RSAAI AATP
Sbjct: 301 SNVITTKVPTNLATLLDLSRMRSAAIAAATP 330

BLAST of MS018410 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.5e-146
Identity = 264/331 (79.76%), Postives = 296/331 (89.43%), Query Frame = 0

Query: 1   MNMATMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDI 60
           MN+++MFMS ++ TQQP INLPPGRLNTN  F  +KLQKEKVV VIGATGTGKSRLSIDI
Sbjct: 1   MNVSSMFMS-EITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDI 60

Query: 61  ATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGL 120
           ATRF SEVINSDKMQVY GLD+VTNK+TKQEQ GVPHHLLG INPF DFSA++FSDMA L
Sbjct: 61  ATRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAML 120

Query: 121 SLDAIAARDRLPIIAGGSNSYIEALIDPNFQSRYECCFLWVDVSMPILCSFVSKRVDKMV 180
           SLDAI++ DRLPIIAGGSNSYIEALID NF+ +YECCFLWVDVSM +L SFVS+RVDKMV
Sbjct: 121 SLDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMV 180

Query: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 240
           ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE Q+KLL+++I+KVK+H
Sbjct: 181 ANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEH 240

Query: 241 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGKESEEAWNKLVAAPSMAIIENFLH 300
           TC+LAL+QLGKIQRL+N+KGW IH+LDATEAFTKQGKE+EEAWNKLV  PS  II+ FLH
Sbjct: 241 TCKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLH 300

Query: 301 QNVFTVKAPTTLASLIGIPRKRSAAIVAATP 332
            N    K PT LASLIG+PRKRSAA+ AATP
Sbjct: 301 PNAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of MS018410 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-85
Identity = 156/301 (51.83%), Postives = 215/301 (71.43%), Query Frame = 0

Query: 5   TMFMSKQLITQQPSINLPPGRLNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSIDIATRF 64
           +M M KQ +   P+++ PP R   N         K+KVV+++GATGTGKSRLS+DIATRF
Sbjct: 6   SMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIATRF 65

Query: 65  SSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINPFYDFSATDFSDMAGLSLDA 124
            +E+INSDK+QV++GLD+VTNK+T +E  GVPHHLLG + P  D +A ++  MA LS+++
Sbjct: 66  RAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLSIES 125

Query: 125 IAARDRLPIIAGGSNSYIEALIDP---NFQSRYECCFLWVDVSMPILCSFVSKRVDKMVA 184
           +  R +LPII GGSNSY+EAL+D     F+SRY+CCFLWVDV++P+L  FVS+RVDKMV 
Sbjct: 126 VLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDKMVE 185

Query: 185 NGMVDEAEKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSLLDEETQDKLLQDAISKVKDH 244
           +GMV+E  +FFD +  DYSRGIK+AIG PEFD +FR +  L+ E +++LL   + ++K +
Sbjct: 186 SGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEIKRN 245

Query: 245 TCRLALRQLGKIQRLKNVKGWKIHQLDATEAFTKQGK--ESEEAWNKLVAAPSMAIIENF 300
           T  LA RQ  KI+RL+ VK W I ++DAT  FTK+    ++  AW +LVA PS   +  F
Sbjct: 246 TFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTVSRF 305

BLAST of MS018410 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 310.1 bits (793), Expect = 2.2e-84
Identity = 157/267 (58.80%), Postives = 205/267 (76.78%), Query Frame = 0

Query: 38  QKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPH 97
           +K+KVV V+GATGTGKSRL+ID+ATRF +E++NSDK+QVY+GLD+VTNKVT +E  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 98  HLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDP--NFQSRY 157
           HLLGT++  Y DF+A DF   A  ++++I  RDR+PIIAGGSNSYIEAL++   +F+ RY
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALVNDCVDFRLRY 150

Query: 158 ECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDP-ARDYSRGIKRAIGVPEFDS 217
            CCFLWVDVS P+L SFVS+RVDKMV  G+VDE  + FDP + DYS GI+RAIGVPE D 
Sbjct: 151 NCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELDE 210

Query: 218 YFRYKSL-LDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDATEAF 277
           + R +      ET ++LL+ AI K+K++TC LA RQL KIQRL     W +H++DATE F
Sbjct: 211 FLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEVF 270

Query: 278 TKQGKESEEAWNKLVAAPSMAIIENFL 300
            ++G+E++EAW+  VA PS   +E FL
Sbjct: 271 LRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of MS018410 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 143/271 (52.77%), Postives = 200/271 (73.80%), Query Frame = 0

Query: 39  KEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHH 98
           KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y+GLDV+TNKVT +E +GVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 99  LLGTI-NPFYDFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALIDPN---FQSRY 158
           LLG   +   + +AT +S +A  ++  ++A ++LPI+AGGSNSYIEAL++ +     + Y
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 159 ECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSY 218
           +CCF+WVDVS+P+L SFVSKRVD+M+  G+++E  + F+P  +YS GI+RAIGVPE   Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 219 FRYKSLLDEETQDKLLQDAISKVKDHTCRLALRQLGKIQRLKNVKGWK--IHQLDATEAF 278
            R +SL+D  T+ K+L  A+  +K +T  LA RQL KIQRL   K WK  +H++DATE F
Sbjct: 213 LRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLH--KKWKMSMHRVDATEVF 272

Query: 279 TKQG-KESEEAWNKLVAAPSMAIIENFLHQN 303
            K+  +E +EAW  LVA PS  I++ F + N
Sbjct: 273 LKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of MS018410 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 241.5 bits (615), Expect = 9.6e-64
Identity = 131/300 (43.67%), Postives = 195/300 (65.00%), Query Frame = 0

Query: 34  KKKLQKEKVVIVIGATGTGKSRLSIDIATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQ 93
           + + +K+KVV+++GATG GKSRLS+D+ATRF SE+INSDK+QVYEGL++ TN++T Q+++
Sbjct: 59  RSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRR 118

Query: 94  GVPHHLLGTINPFY-DFSATDFSDMAGLSLDAIAARDRLPIIAGGSNSYIEALI----DP 153
           GVPHHLLG INP + + +A +F   A   +  I +R ++PIIAGGSNS++ AL+    DP
Sbjct: 119 GVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDP 178

Query: 154 NFQS------------RYECCFLWVDVSMPILCSFVSKRVDKMVANGMVDEAEKFFDPAR 213
            F              RYECCF+WVDVS  +L  ++ +RVD+M+ +GM +E  +F+DP +
Sbjct: 179 KFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVK 238

Query: 214 ---DYSRGIKRAIGVPEFDSYFR----YKSLLDEETQDKLLQD-AISKVKDHTCRLALRQ 273
              +   GI++AIGVPEFD YF+     K ++  +   K   D A+  +K +T  LA RQ
Sbjct: 239 SGLETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQ 298

Query: 274 LGKIQRLKNVKGWKIHQLDATEAF------TKQGKESEEAWNKLVAAPSMAIIENFLHQN 303
           + KI+ LK+  GW+I ++DAT +F      +   K+  E W + V  PS+ I++  L QN
Sbjct: 299 VKKIEMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

BLAST of MS018410 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 236.9 bits (603), Expect = 2.4e-62
Identity = 140/330 (42.42%), Postives = 204/330 (61.82%), Query Frame = 0

Query: 2   NMATMFMSKQLI-TQQPSINLPPGR-LNTNPNFWKKKLQKEKVVIVIGATGTGKSRLSID 61
           N+ + F+S  +I    P + LPP R +        ++  K+KVV+++GATG+GKS LSID
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSID 62

Query: 62  IATRFSSEVINSDKMQVYEGLDVVTNKVTKQEQQGVPHHLLGTINP-FYDFSATDFSDMA 121
           +ATRFS E++NSDK+Q Y+GL V TN+++  E+ GVPHHLLG + P   + + ++F  +A
Sbjct: 63  LATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLA 122

Query: 122 GLSLDAIAARDRLPIIAGGSNSYIEALI----DPN---FQS--------RYECCFLWVDV 181
             S+  I AR  LPIIAGGSNS+I AL+    DP    F S        RYECCFLWVDV
Sbjct: 123 SRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDV 182

Query: 182 SMPILCSFVSKRVDKMVANGMVDEAEKFFDPARDYS----RGIKRAIGVPEFDSYFRYKS 241
           S+ +L  ++SKRVD+M+ +GM +E   F+DP    S     GI + IG+PEFD YF   S
Sbjct: 183 SVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYF---S 242

Query: 242 LLDEETQDKLLQ----------DAISKVKDHTCRLALRQLGKIQRLKNVKGWKIHQLDAT 300
           L   E + K+ +          +A+ ++K++T RLA +Q+ +I +LK+  GW I +LDAT
Sbjct: 243 LYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKS-SGWDIQRLDAT 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150248.13.3e-18399.09adenylate isopentenyltransferase 3, chloroplastic [Momordica charantia][more]
XP_023514910.13.1e-14980.97adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
XP_038899604.14.1e-14981.57adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida][more]
XP_023004596.19.0e-14981.57adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima][more]
KAG7025533.15.9e-14880.66Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q93WC92.1e-8451.83Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID23.1e-8358.80Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID14.5e-7452.77Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF71.1e-6442.32Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID31.3e-6243.67Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1D8Y21.6e-18399.09adenylate isopentenyltransferase 3, chloroplastic OS=Momordica charantia OX=3673... [more]
A0A6J1KSJ24.4e-14981.57adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A0A0K9191.1e-14780.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1[more]
A0A6J1H8C99.1e-14780.36adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A5D3DBM73.5e-14679.76Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT3G63110.11.5e-8551.83isopentenyltransferase 3 [more]
AT5G19040.12.2e-8458.80isopentenyltransferase 5 [more]
AT3G23630.13.2e-7552.77isopentenyltransferase 7 [more]
AT1G68460.19.6e-6443.67isopentenyltransferase 1 [more]
AT3G19160.12.4e-6242.42ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 152..250
e-value: 6.8E-11
score: 42.3
coord: 75..147
e-value: 1.7E-20
score: 73.8
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 165..263
e-value: 1.0E-107
score: 361.4
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 31..305
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 39..299
e-value: 1.0E-107
score: 361.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 38..192
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 31..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018410.1MS018410.1mRNA