MS018374 (gene) Bitter gourd (TR) v1

BLAST of MS018374 vs. NCBI nr
Match: XP_022150297.1 (protein MARD1-like [Momordica charantia])

HSP 1 Score: 465.7 bits (1197), Expect = 2.5e-127
Identity = 240/250 (96.00%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSRAVTGKQALMADHTSQ    SSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP
Sbjct: 1   MLRNRSRAVTGKQALMADHTSQ----SSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE 122
           QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE
Sbjct: 61  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE 120

Query: 123 EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVCEEY 182
           EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEF WNFGGLMTVKEMEVCEEY
Sbjct: 121 EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFCWNFGGLMTVKEMEVCEEY 180

Query: 183 TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNKNNKNAFLSFCYTCKNHL 242
           TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAK     NKNAFLSFCYTCKNHL
Sbjct: 181 TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAK-----NKNAFLSFCYTCKNHL 240

Query: 243 QQTNDIYIYR 253
           QQTNDIYIYR
Sbjct: 241 QQTNDIYIYR 241

BLAST of MS018374 vs. NCBI nr
Match: XP_038896512.1 (protein MARD1-like [Benincasa hispida])

HSP 1 Score: 252.3 bits (643), Expect = 4.4e-63
Identity = 162/254 (63.78%), Postives = 177/254 (69.69%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSRAVTGKQALMAD TSQSSSSS S   SYTKP+ N  FGSPRF+A     FS T 
Sbjct: 1   MLRNRSRAVTGKQALMADQTSQSSSSSPS---SYTKPLKNSFFGSPRFKA-----FSSTN 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE 122
             DHS+ISPTSVLDS PFFSLQNPF   K      PN   +IF  NK    +PIGLALIE
Sbjct: 61  --DHSVISPTSVLDSKPFFSLQNPF---KPNPNTTPNKKVTIFPENKGP-KKPIGLALIE 120

Query: 123 EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVCEEY 182
           +KNDSKP+KSVIFSAKLRVQIPP  PE+ + GG            G LMTV+EMEVCEEY
Sbjct: 121 DKNDSKPTKSVIFSAKLRVQIPP--PESTMDGGGE-------MGNGLLMTVEEMEVCEEY 180

Query: 183 TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVA----KNKNKNNKNAFLSFCYTC 242
           TCV+ HG NAKITHIFDN VV + S  D GS  + ++A    K   K N   FLSFCYTC
Sbjct: 181 TCVKRHGPNAKITHIFDNFVVKS-SVDDYGSPHNFSMADVRKKKIMKKNNTDFLSFCYTC 230

Query: 243 KNHLQQTNDIYIYR 253
           KN LQ TNDIYIYR
Sbjct: 241 KNDLQLTNDIYIYR 230

BLAST of MS018374 vs. NCBI nr
Match: XP_022991961.1 (protein MARD1-like [Cucurbita maxima])

HSP 1 Score: 242.3 bits (617), Expect = 4.6e-60
Identity = 158/260 (60.77%), Postives = 169/260 (65.00%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSRAVTGKQALMAD   QS SSSS    S+TKP+ NP+FGSPRFRA A      T 
Sbjct: 1   MLRNRSRAVTGKQALMADPPFQSPSSSS----SFTKPLKNPIFGSPRFRAFA------TR 60

Query: 63  QIDHS--------LISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS- 122
             DHS        LISPTSVLDS PFFSLQN         P  PN P  IF GNK KIS 
Sbjct: 61  TADHSVLMSPTSVLISPTSVLDSKPFFSLQN---------PNYPNKP--IFPGNKHKISR 120

Query: 123 -EPIGLALIEEKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLMT 182
             PIGLALIE+KND+KP+ S+IFSAKLRV IPPP  +    GG             GLMT
Sbjct: 121 PNPIGLALIEDKNDTKPTTSLIFSAKLRVLIPPPTVDGGGSGG------------SGLMT 180

Query: 183 VKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNKNNKNAFL 242
           VKEMEVCEEYTCVR HG NAK THIFDN VV  KS VD  S         K KNN+  FL
Sbjct: 181 VKEMEVCEEYTCVRRHGPNAKTTHIFDNFVV--KSLVDYDSP--------KKKNNE--FL 215

Query: 243 SFCYTCKNHLQQTNDIYIYR 253
           S+C+TCKNHL  TNDIYIYR
Sbjct: 241 SYCFTCKNHLHHTNDIYIYR 215

BLAST of MS018374 vs. NCBI nr
Match: KAG6593107.1 (Protein MARD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 241.5 bits (615), Expect = 7.8e-60
Identity = 155/257 (60.31%), Postives = 172/257 (66.93%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSR      ALMAD  S               PV N LFGSPRF+       + T 
Sbjct: 1   MLRNRSR------ALMADQAS---------------PVSNSLFGSPRFKPFTP---TTTQ 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS--EPIGLAL 122
            IDHS+ISPTSVLDS PFFSLQNPFS+D +  P  P  P  IF GNKQKIS   PIGLAL
Sbjct: 61  SIDHSVISPTSVLDSKPFFSLQNPFSYDPNTKPNSPKKP--IFPGNKQKISGTNPIGLAL 120

Query: 123 IEEKNDSKPSKSVIFSAKLRVQIPPP-APEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVC 182
           IEEK DS   KSVIF AKLRV+IPPP  P+++V  G  +       + GG+MTVKEMEVC
Sbjct: 121 IEEKYDSTLCKSVIFRAKLRVKIPPPLLPDSSVDDGGGDLFPSGIGD-GGVMTVKEMEVC 180

Query: 183 EEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQK-SMAVAKNK---NKNNKNAFLSFC 242
           EEYTCVRTHG +AKITHIFDN VV  KS VDSG ++ SMA  KNK   NKNNKN FLSFC
Sbjct: 181 EEYTCVRTHGPDAKITHIFDNFVV--KSSVDSGPERISMADLKNKNKNNKNNKNDFLSFC 228

Query: 243 YTCKNHLQQTNDIYIYR 253
           YTC  +L+ T DIYIYR
Sbjct: 241 YTCNRNLELTKDIYIYR 228

BLAST of MS018374 vs. NCBI nr
Match: KAG7025516.1 (Protein MARD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.1 bits (614), Expect = 1.0e-59
Identity = 153/257 (59.53%), Postives = 171/257 (66.54%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSR      ALMAD  S               PV N +FGSPRF+       + T 
Sbjct: 26  MLRNRSR------ALMADQAS---------------PVSNSMFGSPRFKPFTP---TTTQ 85

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS--EPIGLAL 122
            IDHS+ISPTSVLDS PFFSLQNPFS+D +  P  P  P  IF GNKQKIS   PIGLAL
Sbjct: 86  SIDHSVISPTSVLDSKPFFSLQNPFSYDPNTKPNSPKKP--IFPGNKQKISGTNPIGLAL 145

Query: 123 IEEKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVCE 182
           IEEK DS   KSVIF AKLRV+IPP  P+++V  G  +       + GG+MTVKEMEVCE
Sbjct: 146 IEEKYDSTLCKSVIFRAKLRVKIPPLLPDSSVDDGGGDLFPAGIGD-GGMMTVKEMEVCE 205

Query: 183 EYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQK-SMAVAKNK----NKNNKNAFLSFC 242
           EYTCVRTHG +AKITHIFDN VV  KS VDSG ++ SMA  KNK    NKNNKN FLSFC
Sbjct: 206 EYTCVRTHGPDAKITHIFDNFVV--KSSVDSGPERISMADLKNKNNKNNKNNKNDFLSFC 253

Query: 243 YTCKNHLQQTNDIYIYR 253
           YTC  +L+ T DIYIYR
Sbjct: 266 YTCNRNLELTKDIYIYR 253

BLAST of MS018374 vs. ExPASy Swiss-Prot
Match: Q8LGS1 (Protein MARD1 OS=Arabidopsis thaliana OX=3702 GN=MARD1 PE=1 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-18
Identity = 94/267 (35.21%), Postives = 123/267 (46.07%), Query Frame = 0

Query: 3   MLRNRSRA--VTGKQA--LMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGF 62
           MLRN+ RA   T KQ   LMAD       ++   S S        LF SP+FR   +   
Sbjct: 1   MLRNKPRAAVTTKKQTSLLMADQPPPPKPNTCHCSPS--------LFSSPKFRFFTSKMM 60

Query: 63  SETPQIDHSLISPTSVLDSNP-FFSLQNPFSHDKSKLPPPPNSPKSIFS---GNKQKISE 122
                 D SL+SPTS+L++NP  FS +NP              P S F     N Q+   
Sbjct: 61  MTPFDSDFSLVSPTSILEANPSIFSSKNP-------------KPVSYFEPTIPNPQRFHS 120

Query: 123 P--IGLALIEEKNDS------KP-SKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYW 182
           P   GLA + +  DS      KP +K V+F +KLRVQIP  A      G      +    
Sbjct: 121 PDVFGLADLVKDGDSNRDHSRKPVNKMVLFGSKLRVQIPSSADFGTKTGIRYPPCQLSPC 180

Query: 183 NFGGLMTVKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNK 242
               ++ V E++  E+YT V +HG N  ITHIFDN V     FV++              
Sbjct: 181 VQTKVLAVSEIDQTEDYTRVISHGPNPTITHIFDNSV-----FVEATPCSVPLPQPAMET 240

Query: 243 NNKNAFLSFCYTCKNHLQQTNDIYIYR 253
            +  +FLS C+TCK +L Q  DIYIYR
Sbjct: 241 KSTESFLSRCFTCKKNLDQKQDIYIYR 241

BLAST of MS018374 vs. ExPASy Swiss-Prot
Match: Q8L471 (FCS-Like Zinc finger 8 OS=Arabidopsis thaliana OX=3702 GN=FLZ8 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 8.5e-17
Identity = 80/271 (29.52%), Postives = 126/271 (46.49%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           ML+ RSR+   KQALMA+     ++ S +   S T P P        F++     F+E  
Sbjct: 1   MLKKRSRS---KQALMAE-----TNQSQNQKQSKTTPFPRLFTAFSSFKS-----FTE-- 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEP--IGLAL 122
             + ++ SPTS+LD+ PF  L+NPF  D  K   P    K           EP  IGLA+
Sbjct: 61  --NDAVASPTSILDTKPFSVLKNPFGSDNPKTQEPETRLK----------LEPKRIGLAI 120

Query: 123 IEEK-NDSKP-----SKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWN-------- 182
           ++    D  P     S +++F ++LR+++ P +P ++   G   +  +            
Sbjct: 121 VDSLIQDETPEPGPRSGTILFGSQLRIRV-PDSPISSSDFGIKTRNSQPETKKPGSESGL 180

Query: 183 -----FGGLMTVKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAK 242
                  G     +ME+ E+YTCV  HG N +  HIFDNC+V ++  V            
Sbjct: 181 GSPRIISGYFPASDMELSEDYTCVTCHGPNPRTIHIFDNCIVESQPGVVFFRSSDPVNES 240

Query: 243 NKNKNNKNAFLSFCYTCKNHLQQTNDIYIYR 253
           + + +  ++FLS C  CK  L   +DI++YR
Sbjct: 241 DSDYSPPDSFLSCCCNCKKSLGPRDDIFMYR 243

BLAST of MS018374 vs. ExPASy Swiss-Prot
Match: Q9LYE4 (FCS-Like Zinc finger 10 OS=Arabidopsis thaliana OX=3702 GN=FLZ10 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 8.2e-04
Identity = 71/305 (23.28%), Postives = 111/305 (36.39%), Query Frame = 0

Query: 23  SQSSSSSSSSSNSY--TKPVPNPLFGSPRFRALAAMGFSETPQIDHSLISPTSVLDSNPF 82
           SQ S+   ++++ Y  TKPV   L      + ++++   + P    S  SPTS LD   F
Sbjct: 2   SQHSNYQMTTASDYYSTKPV---LSAIRSHKLISSVFEGKCPSDYESAWSPTSPLDFRLF 61

Query: 83  FSLQNPFSHDKSKLPPPPNSPKSIFSGNKQK-ISEPIGLALIEEKND------------- 142
            +L NPF+          +S +SI+ G ++   S  +GL+++    D             
Sbjct: 62  STLGNPFA---------ASSSRSIWRGKQRSWDSGKVGLSIVHSLVDDHHTDSSATIVLP 121

Query: 143 SKPSKSVIFSAKLR---------------------------------VQIPPPAPEAAVR 202
           S  SK++IF + +R                                 + +       +V 
Sbjct: 122 SPDSKNIIFGSLMRSGQKPHLLSQPFTKALMPKDVIPNAVFEIGHDVIDVLELRKSGSVD 181

Query: 203 GGCAEKTEEFYWNFGGLMTVK------------EMEVCEEYTCVRTHGANAKITHIFDNC 252
                  E F  N       K            +ME+ E+YTCV +HG N K TH + + 
Sbjct: 182 AAYCSGAENFSVNNNACQVTKQDPGSLNGGTESDMEISEDYTCVISHGPNPKTTHFYGDQ 241

BLAST of MS018374 vs. ExPASy TrEMBL
Match: A0A6J1DAD0 (protein MARD1-like OS=Momordica charantia OX=3673 GN=LOC111018496 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.2e-127
Identity = 240/250 (96.00%), Postives = 240/250 (96.00%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSRAVTGKQALMADHTSQ    SSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP
Sbjct: 1   MLRNRSRAVTGKQALMADHTSQ----SSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE 122
           QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE
Sbjct: 61  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALIE 120

Query: 123 EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVCEEY 182
           EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEF WNFGGLMTVKEMEVCEEY
Sbjct: 121 EKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFCWNFGGLMTVKEMEVCEEY 180

Query: 183 TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNKNNKNAFLSFCYTCKNHL 242
           TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAK     NKNAFLSFCYTCKNHL
Sbjct: 181 TCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAK-----NKNAFLSFCYTCKNHL 240

Query: 243 QQTNDIYIYR 253
           QQTNDIYIYR
Sbjct: 241 QQTNDIYIYR 241

BLAST of MS018374 vs. ExPASy TrEMBL
Match: A0A6J1JS90 (protein MARD1-like OS=Cucurbita maxima OX=3661 GN=LOC111488453 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 2.2e-60
Identity = 158/260 (60.77%), Postives = 169/260 (65.00%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSRAVTGKQALMAD   QS SSSS    S+TKP+ NP+FGSPRFRA A      T 
Sbjct: 1   MLRNRSRAVTGKQALMADPPFQSPSSSS----SFTKPLKNPIFGSPRFRAFA------TR 60

Query: 63  QIDHS--------LISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS- 122
             DHS        LISPTSVLDS PFFSLQN         P  PN P  IF GNK KIS 
Sbjct: 61  TADHSVLMSPTSVLISPTSVLDSKPFFSLQN---------PNYPNKP--IFPGNKHKISR 120

Query: 123 -EPIGLALIEEKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLMT 182
             PIGLALIE+KND+KP+ S+IFSAKLRV IPPP  +    GG             GLMT
Sbjct: 121 PNPIGLALIEDKNDTKPTTSLIFSAKLRVLIPPPTVDGGGSGG------------SGLMT 180

Query: 183 VKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNKNNKNAFL 242
           VKEMEVCEEYTCVR HG NAK THIFDN VV  KS VD  S         K KNN+  FL
Sbjct: 181 VKEMEVCEEYTCVRRHGPNAKTTHIFDNFVV--KSLVDYDSP--------KKKNNE--FL 215

Query: 243 SFCYTCKNHLQQTNDIYIYR 253
           S+C+TCKNHL  TNDIYIYR
Sbjct: 241 SYCFTCKNHLHHTNDIYIYR 215

BLAST of MS018374 vs. ExPASy TrEMBL
Match: A0A6J1H7G0 (protein MARD1-like OS=Cucurbita moschata OX=3662 GN=LOC111461182 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.4e-59
Identity = 155/257 (60.31%), Postives = 170/257 (66.15%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSR      ALMAD  S               PV N LFGSPRF+       + T 
Sbjct: 1   MLRNRSR------ALMADQAS---------------PVSNSLFGSPRFKPFTP---TTTQ 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS--EPIGLAL 122
            IDHS+ISPTSV DS PFFSLQNPFS D +  P  P  P  IF GNKQKIS   PIGLAL
Sbjct: 61  SIDHSVISPTSVFDSKPFFSLQNPFSCDPNTKPNSPKKP--IFPGNKQKISGKNPIGLAL 120

Query: 123 IEEKNDSKPSKSVIFSAKLRVQIPPP-APEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVC 182
           IEEK DS   KSVIF AKLRV+IPPP  P++AV  G  +       + GG+MTVKEMEVC
Sbjct: 121 IEEKYDSTLCKSVIFRAKLRVKIPPPLLPDSAVDDGGGDLFPSGIGD-GGMMTVKEMEVC 180

Query: 183 EEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQK-SMAVAKNK---NKNNKNAFLSFC 242
           EEYTCVRTHG +AKITHIFDN VV  KS VDSG ++ SMA  KNK   NKNNKN FLSFC
Sbjct: 181 EEYTCVRTHGPDAKITHIFDNFVV--KSSVDSGPERISMADLKNKNKNNKNNKNDFLSFC 228

Query: 243 YTCKNHLQQTNDIYIYR 253
           YTC  +L+ T DIYIYR
Sbjct: 241 YTCNRNLELTKDIYIYR 228

BLAST of MS018374 vs. ExPASy TrEMBL
Match: A0A6J1GQ92 (protein MARD1-like OS=Cucurbita moschata OX=3662 GN=LOC111456488 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 9.3e-59
Identity = 155/261 (59.39%), Postives = 164/261 (62.84%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSRAVTGKQALMAD   QS SSS     SYTKP+ NP+FGSPRFRA A      + 
Sbjct: 1   MLRNRSRAVTGKQALMADPPFQSPSSS-----SYTKPLRNPIFGSPRFRAFATRTADHSV 60

Query: 63  QIDHS---------LISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS 122
            I  S         LISPTSVLDS PFFSLQN                K IF  NK KIS
Sbjct: 61  LISPSSVLTSPSSVLISPTSVLDSKPFFSLQN---------------SKPIFPENKHKIS 120

Query: 123 --EPIGLALIEEKNDSKPSKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWNFGGLM 182
              PIGLALIE+KNDSKP+KS+IFSAKLRV IPPP  +    GG             GLM
Sbjct: 121 HPNPIGLALIEDKNDSKPTKSLIFSAKLRVLIPPPTVDGGGSGG------------SGLM 180

Query: 183 TVKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNKNNKNAF 242
           TVKEMEVCEEYTCVR HG NAK THIFDN VV  KSFVD  SQ        K KNN+  F
Sbjct: 181 TVKEMEVCEEYTCVRRHGPNAKTTHIFDNFVV--KSFVDYDSQ--------KKKNNE--F 217

Query: 243 LSFCYTCKNHLQQTNDIYIYR 253
           LSFC+TC N L  TNDIYIYR
Sbjct: 241 LSFCFTCNNTLHLTNDIYIYR 217

BLAST of MS018374 vs. ExPASy TrEMBL
Match: A0A6J1KPV3 (protein MARD1 OS=Cucurbita maxima OX=3661 GN=LOC111497627 PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.6e-58
Identity = 154/258 (59.69%), Postives = 169/258 (65.50%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           MLRNRSR      ALMAD  S               PV N LFGSPRF+         T 
Sbjct: 1   MLRNRSR------ALMADQAS---------------PVSNSLFGSPRFKPFT----PTTQ 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKIS--EPIGLAL 122
            IDHS+ISPTSVLDS PFFSLQNPFS+D +  P  P  P  IF  NK KIS   PIGLAL
Sbjct: 61  SIDHSVISPTSVLDSKPFFSLQNPFSYDPNTKPNSPKKP--IFPVNKHKISGRNPIGLAL 120

Query: 123 IEEKNDSKPSKSVIFSAKLRVQIPPP-APEAAVRGGCAEKTEEFYWNFGGLMTVKEMEVC 182
           IEEK DS  SKSVIF AKLRV+IPPP  P++AV  G  +       + GG+MTVKEMEVC
Sbjct: 121 IEEKYDSTLSKSVIFRAKLRVKIPPPLLPDSAVDDGGGDLFPSGIGD-GGMMTVKEMEVC 180

Query: 183 EEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQK-SMAVAKNKN----KNNKNAFLSF 242
           EEYTCVRTHG +AKITHIFDN VV  KS VDSG ++ SMA  KNKN    KN KN FLSF
Sbjct: 181 EEYTCVRTHGPDAKITHIFDNFVV--KSSVDSGPERISMADLKNKNKKNKKNKKNDFLSF 228

Query: 243 CYTCKNHLQQTNDIYIYR 253
           CYTC  +L+ T DIYIYR
Sbjct: 241 CYTCNRNLELTKDIYIYR 228

BLAST of MS018374 vs. TAIR 10
Match: AT3G63210.1 (Protein of unknown function (DUF581) )

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-19
Identity = 94/267 (35.21%), Postives = 123/267 (46.07%), Query Frame = 0

Query: 3   MLRNRSRA--VTGKQA--LMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGF 62
           MLRN+ RA   T KQ   LMAD       ++   S S        LF SP+FR   +   
Sbjct: 1   MLRNKPRAAVTTKKQTSLLMADQPPPPKPNTCHCSPS--------LFSSPKFRFFTSKMM 60

Query: 63  SETPQIDHSLISPTSVLDSNP-FFSLQNPFSHDKSKLPPPPNSPKSIFS---GNKQKISE 122
                 D SL+SPTS+L++NP  FS +NP              P S F     N Q+   
Sbjct: 61  MTPFDSDFSLVSPTSILEANPSIFSSKNP-------------KPVSYFEPTIPNPQRFHS 120

Query: 123 P--IGLALIEEKNDS------KP-SKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYW 182
           P   GLA + +  DS      KP +K V+F +KLRVQIP  A      G      +    
Sbjct: 121 PDVFGLADLVKDGDSNRDHSRKPVNKMVLFGSKLRVQIPSSADFGTKTGIRYPPCQLSPC 180

Query: 183 NFGGLMTVKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAKNKNK 242
               ++ V E++  E+YT V +HG N  ITHIFDN V     FV++              
Sbjct: 181 VQTKVLAVSEIDQTEDYTRVISHGPNPTITHIFDNSV-----FVEATPCSVPLPQPAMET 240

Query: 243 NNKNAFLSFCYTCKNHLQQTNDIYIYR 253
            +  +FLS C+TCK +L Q  DIYIYR
Sbjct: 241 KSTESFLSRCFTCKKNLDQKQDIYIYR 241

BLAST of MS018374 vs. TAIR 10
Match: AT3G22550.1 (Protein of unknown function (DUF581) )

HSP 1 Score: 89.0 bits (219), Expect = 6.0e-18
Identity = 80/271 (29.52%), Postives = 126/271 (46.49%), Query Frame = 0

Query: 3   MLRNRSRAVTGKQALMADHTSQSSSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETP 62
           ML+ RSR+   KQALMA+     ++ S +   S T P P        F++     F+E  
Sbjct: 1   MLKKRSRS---KQALMAE-----TNQSQNQKQSKTTPFPRLFTAFSSFKS-----FTE-- 60

Query: 63  QIDHSLISPTSVLDSNPFFSLQNPFSHDKSKLPPPPNSPKSIFSGNKQKISEP--IGLAL 122
             + ++ SPTS+LD+ PF  L+NPF  D  K   P    K           EP  IGLA+
Sbjct: 61  --NDAVASPTSILDTKPFSVLKNPFGSDNPKTQEPETRLK----------LEPKRIGLAI 120

Query: 123 IEEK-NDSKP-----SKSVIFSAKLRVQIPPPAPEAAVRGGCAEKTEEFYWN-------- 182
           ++    D  P     S +++F ++LR+++ P +P ++   G   +  +            
Sbjct: 121 VDSLIQDETPEPGPRSGTILFGSQLRIRV-PDSPISSSDFGIKTRNSQPETKKPGSESGL 180

Query: 183 -----FGGLMTVKEMEVCEEYTCVRTHGANAKITHIFDNCVVNTKSFVDSGSQKSMAVAK 242
                  G     +ME+ E+YTCV  HG N +  HIFDNC+V ++  V            
Sbjct: 181 GSPRIISGYFPASDMELSEDYTCVTCHGPNPRTIHIFDNCIVESQPGVVFFRSSDPVNES 240

Query: 243 NKNKNNKNAFLSFCYTCKNHLQQTNDIYIYR 253
           + + +  ++FLS C  CK  L   +DI++YR
Sbjct: 241 DSDYSPPDSFLSCCCNCKKSLGPRDDIFMYR 243

BLAST of MS018374 vs. TAIR 10
Match: AT5G11460.1 (Protein of unknown function (DUF581) )

HSP 1 Score: 45.8 bits (107), Expect = 5.9e-05
Identity = 71/305 (23.28%), Postives = 111/305 (36.39%), Query Frame = 0

Query: 23  SQSSSSSSSSSNSY--TKPVPNPLFGSPRFRALAAMGFSETPQIDHSLISPTSVLDSNPF 82
           SQ S+   ++++ Y  TKPV   L      + ++++   + P    S  SPTS LD   F
Sbjct: 2   SQHSNYQMTTASDYYSTKPV---LSAIRSHKLISSVFEGKCPSDYESAWSPTSPLDFRLF 61

Query: 83  FSLQNPFSHDKSKLPPPPNSPKSIFSGNKQK-ISEPIGLALIEEKND------------- 142
            +L NPF+          +S +SI+ G ++   S  +GL+++    D             
Sbjct: 62  STLGNPFA---------ASSSRSIWRGKQRSWDSGKVGLSIVHSLVDDHHTDSSATIVLP 121

Query: 143 SKPSKSVIFSAKLR---------------------------------VQIPPPAPEAAVR 202
           S  SK++IF + +R                                 + +       +V 
Sbjct: 122 SPDSKNIIFGSLMRSGQKPHLLSQPFTKALMPKDVIPNAVFEIGHDVIDVLELRKSGSVD 181

Query: 203 GGCAEKTEEFYWNFGGLMTVK------------EMEVCEEYTCVRTHGANAKITHIFDNC 252
                  E F  N       K            +ME+ E+YTCV +HG N K TH + + 
Sbjct: 182 AAYCSGAENFSVNNNACQVTKQDPGSLNGGTESDMEISEDYTCVISHGPNPKTTHFYGDQ 241

BLAST of MS018374 vs. TAIR 10
Match: AT1G79970.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF581) (TAIR:AT2G25690.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 45.1 bits (105), Expect = 1.0e-04
Identity = 48/193 (24.87%), Postives = 82/193 (42.49%), Query Frame = 0

Query: 26  SSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETPQIDHSLISPTSVLDSNPFFSLQN 85
           +S S SS  S       PLF    F  +A  G S+  +      SPTS LD   F  L +
Sbjct: 4   ASDSDSSDESLKHNSTPPLFSISGFFCVAKGGDSDPGR------SPTSPLDFGVFGGLFS 63

Query: 86  PFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALI----EEKNDSKPSKSVIFSAKLRV 145
           P S   S L               + +S  +GL+L+    E  +D      ++ + +++V
Sbjct: 64  PRSSSSSPL------------HRNKWLSHKVGLSLLSSSFELGDDPFRRDYIVLAPQVKV 123

Query: 146 Q-----IPPPAPEAAV-----RGGCAEKTEEFYWNFGGLMTVKEMEVCEEYTCVRTHGAN 205
                  P  + +  V     R   +    +    +   ++ +EM + E+YTC+ +HG N
Sbjct: 124 NNVNTATPKLSSDPCVIVEEPRRSSSSSPMDIISTYSRSLSGREMALSEDYTCIISHGPN 178

BLAST of MS018374 vs. TAIR 10
Match: AT1G79970.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF581) (TAIR:AT2G25690.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 45.1 bits (105), Expect = 1.0e-04
Identity = 48/193 (24.87%), Postives = 82/193 (42.49%), Query Frame = 0

Query: 26  SSSSSSSSNSYTKPVPNPLFGSPRFRALAAMGFSETPQIDHSLISPTSVLDSNPFFSLQN 85
           +S S SS  S       PLF    F  +A  G S+  +      SPTS LD   F  L +
Sbjct: 4   ASDSDSSDESLKHNSTPPLFSISGFFCVAKGGDSDPGR------SPTSPLDFGVFGGLFS 63

Query: 86  PFSHDKSKLPPPPNSPKSIFSGNKQKISEPIGLALI----EEKNDSKPSKSVIFSAKLRV 145
           P S   S L               + +S  +GL+L+    E  +D      ++ + +++V
Sbjct: 64  PRSSSSSPL------------HRNKWLSHKVGLSLLSSSFELGDDPFRRDYIVLAPQVKV 123

Query: 146 Q-----IPPPAPEAAV-----RGGCAEKTEEFYWNFGGLMTVKEMEVCEEYTCVRTHGAN 205
                  P  + +  V     R   +    +    +   ++ +EM + E+YTC+ +HG N
Sbjct: 124 NNVNTATPKLSSDPCVIVEEPRRSSSSSPMDIISTYSRSLSGREMALSEDYTCIISHGPN 178