MS018372 (gene) Bitter gourd (TR) v1

Overview
NameMS018372
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box/kelch-repeat protein SKIP30-like
Locationscaffold342: 363511 .. 364548 (+)
RNA-Seq ExpressionMS018372
SyntenyMS018372
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGGTCTGATTGAAGGCCTCCCTGATGCAATTGCCCTTAGATGCCTTGCTCGTGTCCCATATTACCTCCACCCCAAATTGGAACTCGTTTCTCGCTCTTGGAAAGCCGCGTTTCGTAGTGCTGAACTGTTTAGAGCTCGACAAGAGGTTGGTTCATCTGAGGATTTACTGTGTGTATGCTCTTATGACCCAAATAATACATGGCAGCTTTATGATCCTCTCCGAGATCTTTGGATCACCCTCCCTGAACTCCCCTCAAAACGAAGACATCTTTCGCATTTTGGGGCTGCCTCCACTTCTCAATACCTGTTTGTTCTCGGTGGTCGTAGCGATGCTATTGATCCATTGACCGGTGACCATGACGACAACTTATCGACCAATGAGGTATGGTCCTTTGACCCTGTAACACGAAACTGGTTGATGCGAGCACCAATGCTCGTACCACGTGCTATGTTTGCCTGCTGTGTTTTGGAAGGGAAGATCATTGTTGCAGGCGGCTTCACCAGCACATCAAAATCAACTTCCAAGACTGAAATATATGACTCTGAGAAGGATCTTTGGGTTCCGTTACCTGATCTACTTCAAACCCATGATTCAACCTGCATTGGAGTAGTGATAGGAGGAAAAGTGCACATTATGTACAAGGGGCAATCCACAGTACAAATTTTTGACACTTTGGAGCTGAAATGGAGAGTTGAGGATTACGGTTGGCTTCCCGGCCCCATGGCTGCCGTTCAAGATTCACTCTATGTCATGTGCCTAGGATTGATCTTCAAGCAGGATGGACGAGACAGACAGGTTGTCATTCCGGCGGTATCTCAGTTTCTCCATAGAATCGGGATGGCGATGATTGGGTTTAGAGGCGAATTGTATGTGATTGGAGGAGTAGTTCGCCCCGTCCGCCTCGATGCGGATCTCGTAAAACTGTCTGACGTGCATGTTCTTAACCTGAGGGGTGAGAAACCAACTTGGTATTGTGTTGCTCCGATGAGTCGATGCCATGGAACAGTTCTTGGGTGCACAGAGTTGAGAATT

mRNA sequence

ATGTCTGGTCTGATTGAAGGCCTCCCTGATGCAATTGCCCTTAGATGCCTTGCTCGTGTCCCATATTACCTCCACCCCAAATTGGAACTCGTTTCTCGCTCTTGGAAAGCCGCGTTTCGTAGTGCTGAACTGTTTAGAGCTCGACAAGAGGTTGGTTCATCTGAGGATTTACTGTGTGTATGCTCTTATGACCCAAATAATACATGGCAGCTTTATGATCCTCTCCGAGATCTTTGGATCACCCTCCCTGAACTCCCCTCAAAACGAAGACATCTTTCGCATTTTGGGGCTGCCTCCACTTCTCAATACCTGTTTGTTCTCGGTGGTCGTAGCGATGCTATTGATCCATTGACCGGTGACCATGACGACAACTTATCGACCAATGAGGTATGGTCCTTTGACCCTGTAACACGAAACTGGTTGATGCGAGCACCAATGCTCGTACCACGTGCTATGTTTGCCTGCTGTGTTTTGGAAGGGAAGATCATTGTTGCAGGCGGCTTCACCAGCACATCAAAATCAACTTCCAAGACTGAAATATATGACTCTGAGAAGGATCTTTGGGTTCCGTTACCTGATCTACTTCAAACCCATGATTCAACCTGCATTGGAGTAGTGATAGGAGGAAAAGTGCACATTATGTACAAGGGGCAATCCACAGTACAAATTTTTGACACTTTGGAGCTGAAATGGAGAGTTGAGGATTACGGTTGGCTTCCCGGCCCCATGGCTGCCGTTCAAGATTCACTCTATGTCATGTGCCTAGGATTGATCTTCAAGCAGGATGGACGAGACAGACAGGTTGTCATTCCGGCGGTATCTCAGTTTCTCCATAGAATCGGGATGGCGATGATTGGGTTTAGAGGCGAATTGTATGTGATTGGAGGAGTAGTTCGCCCCGTCCGCCTCGATGCGGATCTCGTAAAACTGTCTGACGTGCATGTTCTTAACCTGAGGGGTGAGAAACCAACTTGGTATTGTGTTGCTCCGATGAGTCGATGCCATGGAACAGTTCTTGGGTGCACAGAGTTGAGAATT

Coding sequence (CDS)

ATGTCTGGTCTGATTGAAGGCCTCCCTGATGCAATTGCCCTTAGATGCCTTGCTCGTGTCCCATATTACCTCCACCCCAAATTGGAACTCGTTTCTCGCTCTTGGAAAGCCGCGTTTCGTAGTGCTGAACTGTTTAGAGCTCGACAAGAGGTTGGTTCATCTGAGGATTTACTGTGTGTATGCTCTTATGACCCAAATAATACATGGCAGCTTTATGATCCTCTCCGAGATCTTTGGATCACCCTCCCTGAACTCCCCTCAAAACGAAGACATCTTTCGCATTTTGGGGCTGCCTCCACTTCTCAATACCTGTTTGTTCTCGGTGGTCGTAGCGATGCTATTGATCCATTGACCGGTGACCATGACGACAACTTATCGACCAATGAGGTATGGTCCTTTGACCCTGTAACACGAAACTGGTTGATGCGAGCACCAATGCTCGTACCACGTGCTATGTTTGCCTGCTGTGTTTTGGAAGGGAAGATCATTGTTGCAGGCGGCTTCACCAGCACATCAAAATCAACTTCCAAGACTGAAATATATGACTCTGAGAAGGATCTTTGGGTTCCGTTACCTGATCTACTTCAAACCCATGATTCAACCTGCATTGGAGTAGTGATAGGAGGAAAAGTGCACATTATGTACAAGGGGCAATCCACAGTACAAATTTTTGACACTTTGGAGCTGAAATGGAGAGTTGAGGATTACGGTTGGCTTCCCGGCCCCATGGCTGCCGTTCAAGATTCACTCTATGTCATGTGCCTAGGATTGATCTTCAAGCAGGATGGACGAGACAGACAGGTTGTCATTCCGGCGGTATCTCAGTTTCTCCATAGAATCGGGATGGCGATGATTGGGTTTAGAGGCGAATTGTATGTGATTGGAGGAGTAGTTCGCCCCGTCCGCCTCGATGCGGATCTCGTAAAACTGTCTGACGTGCATGTTCTTAACCTGAGGGGTGAGAAACCAACTTGGTATTGTGTTGCTCCGATGAGTCGATGCCATGGAACAGTTCTTGGGTGCACAGAGTTGAGAATT

Protein sequence

MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGDHDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEIYDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLPGPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRPVRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI
Homology
BLAST of MS018372 vs. NCBI nr
Match: XP_022150165.1 (F-box/kelch-repeat protein SKIP30-like [Momordica charantia])

HSP 1 Score: 726.9 bits (1875), Expect = 8.4e-206
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD
Sbjct: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
           HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI
Sbjct: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP
Sbjct: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP
Sbjct: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
           VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI
Sbjct: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 346

BLAST of MS018372 vs. NCBI nr
Match: KAG6575922.1 (F-box/kelch-repeat protein SKIP30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 588.2 bits (1515), Expect = 4.7e-164
Identity = 274/346 (79.19%), Postives = 305/346 (88.15%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI++RCLA VPYYLHPKLELVSRSWKAA RS ELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPL++LW+TLPELPSK RHL++FGA STSQ LFVLGGR DA+DP+TGD
Sbjct: 61  CSYDPNNTWQLYDPLQNLWMTLPELPSKIRHLANFGAVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            DDN STNEVWSFDPVTR W MRAPMLVPR+ FACCV++GKI+VAGGFTS SKSTS+ E+
Sbjct: 121 RDDNSSTNEVWSFDPVTRTWSMRAPMLVPRSTFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YDSEKD+W+PLPDLL THDSTCIG++IGG++HIMY G STVQ+FD+LELKWRVEDYGWLP
Sbjct: 181 YDSEKDVWIPLPDLLHTHDSTCIGLMIGGRMHIMYGGVSTVQVFDSLELKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           G  A V  SLY M  GL+FKQ+ RD Q V+ A SQF+ RIGMAM+GFRGELYVIGGVVRP
Sbjct: 241 GHKAVVGGSLYTMSQGLVFKQEERDAQAVVSA-SQFVQRIGMAMVGFRGELYVIGGVVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            R + DLVKLSDVHVL +R EKP+W C APMSRC GTVLGCT  RI
Sbjct: 301 ERFNGDLVKLSDVHVLQVRNEKPSWSCAAPMSRCSGTVLGCTVWRI 345

BLAST of MS018372 vs. NCBI nr
Match: KAG7014455.1 (F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 585.5 bits (1508), Expect = 3.0e-163
Identity = 272/342 (79.53%), Postives = 303/342 (88.60%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI++RCLA VPYYLHPKLELVSRSWKAA RS ELFRARQEVGSSEDLLCV
Sbjct: 63  MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 122

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPL++LW+TLPELPSK RHL++FGA STSQ LFVLGGR DA+DP+TGD
Sbjct: 123 CSYDPNNTWQLYDPLQNLWMTLPELPSKIRHLANFGAVSTSQKLFVLGGRGDAVDPVTGD 182

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            DDN STNEVWSFDPVTR W MRAPMLVPR+ FACCV++GKI+VAGGFTS SKSTS+ E+
Sbjct: 183 RDDNSSTNEVWSFDPVTRTWSMRAPMLVPRSTFACCVVDGKIVVAGGFTSLSKSTSQAEM 242

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YDSEKD+W+PLPDLL THDSTCIG++IGG++HIMY G STVQ+FD+LELKWRVEDYGWLP
Sbjct: 243 YDSEKDVWIPLPDLLHTHDSTCIGLMIGGRMHIMYGGVSTVQVFDSLELKWRVEDYGWLP 302

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           G  A V  SLY M  GL+FKQ+ RD Q V+ A SQF+ RIGMAM+GFRGELYVIGGVVRP
Sbjct: 303 GHKAVVGGSLYTMSQGLVFKQEERDAQAVVSA-SQFVQRIGMAMVGFRGELYVIGGVVRP 362

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCT 343
            R + DLVKLSDVHVL +R EKP+W C APMSRC GTVLGCT
Sbjct: 363 ERFNGDLVKLSDVHVLQVRNEKPSWSCAAPMSRCSGTVLGCT 403

BLAST of MS018372 vs. NCBI nr
Match: XP_022953500.1 (F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022953501.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata])

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-163
Identity = 271/346 (78.32%), Postives = 303/346 (87.57%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI++RCLA VPYYLHPKLELVSRSWKAA RS ELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPL++LW+TLPELPSK  HL++FG  STSQ LFVLGGR DA+DP+TGD
Sbjct: 61  CSYDPNNTWQLYDPLQNLWMTLPELPSKITHLANFGVVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            +DN STNEVWSFDPVTR W MRAPMLVPR  FACCV++GKI+VAGGFTS SKSTS+ E+
Sbjct: 121 REDNSSTNEVWSFDPVTRTWSMRAPMLVPRYTFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD+EKD+W+PLPDLL THDSTCIG++IGGK+H+MY G STVQ+FD+LELKWRVEDYGWLP
Sbjct: 181 YDNEKDVWIPLPDLLHTHDSTCIGLMIGGKMHVMYGGVSTVQVFDSLELKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           G  A V  SLY M  GL+FKQ+ RD Q V+ A SQF+ RIGMA++GFRGELYVIGGVVRP
Sbjct: 241 GHKAVVGGSLYTMSQGLVFKQEERDAQAVVSA-SQFVQRIGMAIVGFRGELYVIGGVVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            R D DLVKLSDVHVL +R EKP+WYC APMSRC GTVLGCT  RI
Sbjct: 301 ERFDGDLVKLSDVHVLKVRNEKPSWYCAAPMSRCSGTVLGCTVWRI 345

BLAST of MS018372 vs. NCBI nr
Match: XP_023547905.1 (F-box/kelch-repeat protein SKIP30-like [Cucurbita pepo subsp. pepo] >XP_023547906.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 581.3 bits (1497), Expect = 5.7e-162
Identity = 271/346 (78.32%), Postives = 305/346 (88.15%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI++RCLA VPYYLHPKLELVSRSWKAA RS ELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPL++LW+T+PELPSK RHL++FGA STSQ LFVLGG  DAIDP+TGD
Sbjct: 61  CSYDPNNTWQLYDPLQNLWMTIPELPSKIRHLANFGAVSTSQKLFVLGGCGDAIDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            DDN STNEVWSFDPVTR W +RAPMLVPR+ FACCV++GKI+VAGGFTS SKSTS+ E+
Sbjct: 121 RDDNSSTNEVWSFDPVTRTWSLRAPMLVPRSTFACCVVDGKIVVAGGFTSISKSTSQAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD+EKD+W+PLPDLL THDSTCIG++IGGK++IMY G STVQ+FD+LELKWRVEDYGWLP
Sbjct: 181 YDNEKDVWIPLPDLLHTHDSTCIGLMIGGKMNIMYGGVSTVQVFDSLELKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           G  A V  SLY M  GL+FKQ+ RD Q V+ A SQF+ RIGMA++GFRGELYVIGGVVRP
Sbjct: 241 GHKAVVGGSLYTMSQGLVFKQEERDVQAVVSA-SQFVQRIGMAIVGFRGELYVIGGVVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            R D DLVKLSDVHVL +R EKP+W C APMS+C GTVLGCT LRI
Sbjct: 301 ERFDGDLVKLSDVHVLKVRNEKPSWCCAAPMSQCSGTVLGCTVLRI 345

BLAST of MS018372 vs. ExPASy Swiss-Prot
Match: Q9M1W7 (F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana OX=3702 GN=SKIP30 PE=1 SV=2)

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-125
Identity = 203/346 (58.67%), Postives = 263/346 (76.01%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGL++G+P+A+ALRCLA VP +LHP LELVSRSW+AA RS ELFR R+E+ SSE LLCV
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           C++DP N WQ+Y P  D W+TLP LPS+ RHL+HFGA +T+  LFVLGG SDA+ P+TGD
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
           HD   +T++VWS+D V R W  RA MLVPRAMFACCVL+GKI+VAGGFT+  KS S  E+
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD E D+W  +PDL QTH+S C G+V+ GKVH+++KG STVQ+ ++++L W V+DYGW  
Sbjct: 188 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDVKDYGWPQ 247

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           GPM  V+D LYVM  GL+FKQ+G D   ++ + S+F  RIGMAM     E+ ++GGV+ P
Sbjct: 248 GPMVVVEDVLYVMSHGLVFKQEG-DTWKMVASASEFKRRIGMAMTSLSDEVLIVGGVIGP 307

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            RL+ D+  LSDV  L +  ++P W  VAPM+RC GT+LGCT+L I
Sbjct: 308 DRLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 352

BLAST of MS018372 vs. ExPASy Swiss-Prot
Match: Q0WW40 (F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g16250 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 117/373 (31.37%), Postives = 161/373 (43.16%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCSY 63
           +I GLPD +ALRC+A++ +  H  LE VSR W+   R A+    +   G S   L V + 
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVLTE 67

Query: 64  DPNNTWQLYDPLRDLWITLPELPSKRR--HLSHFGAASTSQYLFVLGGRSDAIDPLTGDH 123
              N W  YDP  D W  LP   + +   H S F     S  L V+GG   A    +  H
Sbjct: 68  RSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVCVSNCLLVIGG-CYAPSVSSFPH 127

Query: 124 DDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTST-SKSTSKTEI 183
              + T +V  FDP  + W M A M  PR  FAC  + GK+ VAGG   T S+     E+
Sbjct: 128 QKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGIPSAEV 187

Query: 184 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIM-----YKGQSTVQIFDTLELKWRVED 243
           YD   D W  LP  +      C G+   G  H++     +  Q++ ++F+  ++ W   +
Sbjct: 188 YDPVADRWEELP-AMPRPQMDCSGLSYRGCFHVLSDQVGFAEQNSSEVFNPRDMTWSTVE 247

Query: 244 YGWLPGPMA---AVQ----DSLYVMC---LGLIFKQD---------GRDRQVVIPAVSQF 303
             W P   A   AVQ    D +Y +      LI  +D         G    VV+P   + 
Sbjct: 248 DVW-PFSRAMQFAVQVMKNDRVYTIVDWGESLIKTRDTDEGEWYNVGSVPSVVLPNHPRE 307

Query: 304 LHRIGMAMIGFRGELYVIGGVVRPVRLDA----DLVKLSDVHVLNLRGEKPTWYCVAPMS 345
           L   G      R ELYVIGG V           D+V+L  V V N       W    PM 
Sbjct: 308 LEAFGYGFAALRNELYVIGGKVLKWEESGAGRFDIVRLPVVRVCNPLDRPLNWRETKPMC 367

BLAST of MS018372 vs. ExPASy Swiss-Prot
Match: Q93W93 (F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g55270 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.4e-22
Identity = 78/258 (30.23%), Postives = 115/258 (44.57%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCSY 63
           L+ GLPD +A+ CL RVP   H KL LV + W         +  R+ +G SE+ + V   
Sbjct: 78  LLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLGMSEEWVYVFKR 137

Query: 64  DPNN--TWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQ-YLFVLGGRSDAIDPLTGD 123
           D +   +W  +DP+  LW  LP +P +      FG A  S  +L++ GG+    DPL G 
Sbjct: 138 DRDGKISWNTFDPISQLWQPLPPVPREYSEAVGFGCAVLSGCHLYLFGGK----DPLRG- 197

Query: 124 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKT-E 183
                S   V  ++  T  W     ML  R  F CCV+   + VAGG     + T ++ E
Sbjct: 198 -----SMRRVIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGECEGIQRTLRSAE 257

Query: 184 IYDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTV---QIFDTLELKWRVEDY 243
           +YD  K+ W  + D + T     IGVV   K  +   G   +   + +D     W     
Sbjct: 258 VYDPNKNRWSFIAD-MSTAMVPLIGVVYDKKWFLKGLGSHQLVMSEAYDPEVNSWSPVSD 317

Query: 244 GWLPG---PMAAVQDSLY 252
           G + G   P  ++   LY
Sbjct: 318 GMVAGWRNPCTSLNGRLY 324

BLAST of MS018372 vs. ExPASy Swiss-Prot
Match: Q9FKJ0 (F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana OX=3702 GN=At5g60570 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 8.1e-18
Identity = 70/239 (29.29%), Postives = 104/239 (43.51%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLL-CVCS 63
           ++ GL D +AL CLA VP   +P L  V++ +     S  LF  R+E+G  E L+  VC 
Sbjct: 49  VLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVEYLVFMVC- 108

Query: 64  YDPNNTWQLYDPLRDLWITLPELPSKR--RHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 123
            DP   W ++ P++  W+ LP++P      H      A   + L V G            
Sbjct: 109 -DPRG-WLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDE-LLVFG------------ 168

Query: 124 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 183
               L    +W +   +R W+    M  PR +FA   L G  IVAGG        +  E+
Sbjct: 169 --RELFQFAIWKYSLRSRCWVKCEGMHRPRCLFASGSLGGIAIVAGGTDMNGNILASAEL 228

Query: 184 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQI-------FDTLELKWR 233
           YDS    W  LP+ + +    C G  + GK +++  G S+  +       FD    KWR
Sbjct: 229 YDSSSGRWEMLPN-MHSPRRLCSGFFMDGKFYVI-GGMSSPNVSVTFGEEFDLETRKWR 267

BLAST of MS018372 vs. ExPASy Swiss-Prot
Match: Q9LM55 (F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g22040 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.1e-17
Identity = 61/223 (27.35%), Postives = 99/223 (44.39%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCS- 63
           LI  LPD ++++ LAR+P   +  + LVSR W++A  ++E++  R+E+G +E+ L V + 
Sbjct: 43  LIPSLPDELSIQILARLPRICYSSVRLVSRRWRSAVSTSEVYSLRKELGRTEEWLYVLTK 102

Query: 64  -YDPNNTWQLYDPLRDLWITLPELP------SKRRHL------------------SHFGA 123
            ++    W   DP+   W  LP +P        R+ L                  S  G 
Sbjct: 103 GHEDKLLWYALDPVSTKWQRLPPMPVVVYEEESRKSLSGLWNMITPSFNVGAIVRSFLGR 162

Query: 124 ASTSQYLFVLGGRSDAIDP---LTGDHDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFA 183
             +S+ +   G    A+D    + G    + + + VW FDP+  +W   + ML  RA   
Sbjct: 163 RDSSEQMPFCGCAIGAVDGGLYVIGGLSRSKTVSCVWRFDPILNSWSEVSSMLASRAYSK 222

Query: 184 CCVLEGKIIVAGGFTSTSKSTS---KTEIYDSEKDLWVPLPDL 195
             VL  K+ V GG        S     E+YD   D W  +P +
Sbjct: 223 TGVLNKKLYVVGGVDRGRGGLSPLQSAEVYDPSTDAWSEVPSM 265

BLAST of MS018372 vs. ExPASy TrEMBL
Match: A0A6J1D7Q9 (F-box/kelch-repeat protein SKIP30-like OS=Momordica charantia OX=3673 GN=LOC111018411 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.1e-206
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD
Sbjct: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
           HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI
Sbjct: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP
Sbjct: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP
Sbjct: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
           VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI
Sbjct: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 346

BLAST of MS018372 vs. ExPASy TrEMBL
Match: A0A6J1GPT2 (F-box/kelch-repeat protein SKIP30-like OS=Cucurbita moschata OX=3662 GN=LOC111456033 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.9e-163
Identity = 271/346 (78.32%), Postives = 303/346 (87.57%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI++RCLA VPYYLHPKLELVSRSWKAA RS ELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTWQLYDPL++LW+TLPELPSK  HL++FG  STSQ LFVLGGR DA+DP+TGD
Sbjct: 61  CSYDPNNTWQLYDPLQNLWMTLPELPSKITHLANFGVVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            +DN STNEVWSFDPVTR W MRAPMLVPR  FACCV++GKI+VAGGFTS SKSTS+ E+
Sbjct: 121 REDNSSTNEVWSFDPVTRTWSMRAPMLVPRYTFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD+EKD+W+PLPDLL THDSTCIG++IGGK+H+MY G STVQ+FD+LELKWRVEDYGWLP
Sbjct: 181 YDNEKDVWIPLPDLLHTHDSTCIGLMIGGKMHVMYGGVSTVQVFDSLELKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           G  A V  SLY M  GL+FKQ+ RD Q V+ A SQF+ RIGMA++GFRGELYVIGGVVRP
Sbjct: 241 GHKAVVGGSLYTMSQGLVFKQEERDAQAVVSA-SQFVQRIGMAIVGFRGELYVIGGVVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            R D DLVKLSDVHVL +R EKP+WYC APMSRC GTVLGCT  RI
Sbjct: 301 ERFDGDLVKLSDVHVLKVRNEKPSWYCAAPMSRCSGTVLGCTVWRI 345

BLAST of MS018372 vs. ExPASy TrEMBL
Match: A0A6J1JW98 (F-box/kelch-repeat protein SKIP30-like OS=Cucurbita maxima OX=3661 GN=LOC111488452 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.4e-160
Identity = 266/346 (76.88%), Postives = 301/346 (86.99%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI++RCLA VPYYLHPKLELVSRSWKAA RS ELFRARQEVGSSEDLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSYDPNNTW+LYDPL++LW+TLPELPSK +HL++FG  STSQ LFVLGGR DA+DP+TGD
Sbjct: 61  CSYDPNNTWKLYDPLQNLWMTLPELPSKIKHLANFGVVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            ++  STNEVWSFDPVTR W MRAPMLVPR+MFACCV++GKI+VAGGFTS SKSTS+ E+
Sbjct: 121 REETSSTNEVWSFDPVTRMWSMRAPMLVPRSMFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD EKD+W+PLPDLL T DSTCIG++IGGK+HIMY G STVQ+FD+LELKWRVE YGWLP
Sbjct: 181 YDIEKDVWIPLPDLLHTRDSTCIGLMIGGKMHIMYGGVSTVQVFDSLELKWRVEYYGWLP 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           G  A V  SLY M  GL+FKQ+ R  Q V+ A SQF+ RIGMAM+GFRGELYVIGG+VRP
Sbjct: 241 GHKAVVGGSLYTMSQGLVFKQEERGAQAVVSA-SQFVQRIGMAMVGFRGELYVIGGIVRP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            R D DLVKLSDVHVL +R EKP+WYC AP SRC GT+LGCT LRI
Sbjct: 301 ERFDGDLVKLSDVHVLKVRNEKPSWYCAAPTSRCSGTILGCTVLRI 345

BLAST of MS018372 vs. ExPASy TrEMBL
Match: A0A0A0K7B1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G387780 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.8e-153
Identity = 262/348 (75.29%), Postives = 292/348 (83.91%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI+LRCLA +PYYLHPKLELVSRSWKAA RS ELFRARQEVG SED LCV
Sbjct: 1   MSGLIEGLPDAISLRCLAYIPYYLHPKLELVSRSWKAAIRSVELFRARQEVGFSEDFLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSY PNNTWQLYDPL + W+TLPELPSKR HL +F A STSQ LFVLGGRSDA+DP+TGD
Sbjct: 61  CSYHPNNTWQLYDPLPNRWMTLPELPSKRMHLGNFCAVSTSQKLFVLGGRSDAVDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            DDN STNEVWSFDP+TR W MRAPMLVPRAMFACCV++GKIIVAGGFTS SKSTSK E+
Sbjct: 121 RDDNFSTNEVWSFDPITRTWSMRAPMLVPRAMFACCVVDGKIIVAGGFTSKSKSTSKAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YDSEKD+W PLPDLLQTHDSTCIG+V+ GK+HI+Y   STVQ+ D+ E+KWRVEDYGW+ 
Sbjct: 181 YDSEKDVWTPLPDLLQTHDSTCIGLVVRGKMHIVYNKVSTVQVLDSSEMKWRVEDYGWVL 240

Query: 241 GPMAAVQDSLYVM--CLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVV 300
           G  A V DSLYVM    G++FKQ GR  +V+  A +QF  RIGMA++GFRG+LY IGG +
Sbjct: 241 GLKAVVGDSLYVMNPLEGVVFKQYGRTWKVIALA-TQFAQRIGMAVVGFRGDLYAIGGGI 300

Query: 301 RPVRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            P R   DL KLSDVHVLNLR E+PTW C A MSRC GTVLGCTELRI
Sbjct: 301 HPNRTGGDLTKLSDVHVLNLRDEEPTWRCAAQMSRCQGTVLGCTELRI 347

BLAST of MS018372 vs. ExPASy TrEMBL
Match: A0A1S3BQL6 (F-box/kelch-repeat protein SKIP30-like OS=Cucumis melo OX=3656 GN=LOC103492650 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.0e-153
Identity = 264/348 (75.86%), Postives = 292/348 (83.91%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGLIEGLPDAI+LRCLA +PYYLHPKL LVSRSWKAA RS ELFRARQEVG SED LCV
Sbjct: 1   MSGLIEGLPDAISLRCLAYIPYYLHPKLALVSRSWKAAIRSVELFRARQEVGFSEDFLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           CSY PNNTWQLYDPL + W+TLPELPSKR HL +F   STSQ LFVLGGRS A+DP+TGD
Sbjct: 61  CSYHPNNTWQLYDPLPNHWMTLPELPSKRMHLGNFCTVSTSQKLFVLGGRSIAVDPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
            DDN STNEVWSFDP+TR W MRAPMLVPRAMFACCV++GKIIVAGGFTS SKSTSK E+
Sbjct: 121 RDDNFSTNEVWSFDPITRMWSMRAPMLVPRAMFACCVVDGKIIVAGGFTSKSKSTSKAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YDSEKD+W  LPDLLQTHDSTC G VI GK+HI+Y G STVQ+ D+LE+KWRVEDYGWLP
Sbjct: 181 YDSEKDVWTLLPDLLQTHDSTCNGWVIRGKMHIVYNGVSTVQVLDSLEMKWRVEDYGWLP 240

Query: 241 GPMAAVQDSLYVMCL--GLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVV 300
           G  A V DSLYVM L  G++FKQ GR  +V + A +QFL RIGMA++GFRG+LY IGGV+
Sbjct: 241 GLKAVVGDSLYVMSLTQGVVFKQYGRAWKVFVLA-TQFLQRIGMAVVGFRGDLYAIGGVI 300

Query: 301 RPVRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            P R   DL KLSDVHVLNLR E+PTW+  A MSRC GTVLGCTELRI
Sbjct: 301 HPNRTGGDLTKLSDVHVLNLRDEEPTWHSAAQMSRCQGTVLGCTELRI 347

BLAST of MS018372 vs. TAIR 10
Match: AT3G63220.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 450.7 bits (1158), Expect = 1.1e-126
Identity = 203/346 (58.67%), Postives = 263/346 (76.01%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGL++G+P+A+ALRCLA VP +LHP LELVSRSW+AA RS ELFR R+E+ SSE LLCV
Sbjct: 1   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 60

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           C++DP N WQ+Y P  D W+TLP LPS+ RHL+HFGA +T+  LFVLGG SDA+ P+TGD
Sbjct: 61  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 120

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
           HD   +T++VWS+D V R W  RA MLVPRAMFACCVL+GKI+VAGGFT+  KS S  E+
Sbjct: 121 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 180

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD E D+W  +PDL QTH+S C G+V+ GKVH+++KG STVQ+ ++++L W V+DYGW  
Sbjct: 181 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDVKDYGWPQ 240

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           GPM  V+D LYVM  GL+FKQ+G D   ++ + S+F  RIGMAM     E+ ++GGV+ P
Sbjct: 241 GPMVVVEDVLYVMSHGLVFKQEG-DTWKMVASASEFKRRIGMAMTSLSDEVLIVGGVIGP 300

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            RL+ D+  LSDV  L +  ++P W  VAPM+RC GT+LGCT+L I
Sbjct: 301 DRLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 345

BLAST of MS018372 vs. TAIR 10
Match: AT3G63220.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 450.7 bits (1158), Expect = 1.1e-126
Identity = 203/346 (58.67%), Postives = 263/346 (76.01%), Query Frame = 0

Query: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60
           MSGL++G+P+A+ALRCLA VP +LHP LELVSRSW+AA RS ELFR R+E+ SSE LLCV
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120
           C++DP N WQ+Y P  D W+TLP LPS+ RHL+HFGA +T+  LFVLGG SDA+ P+TGD
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180
           HD   +T++VWS+D V R W  RA MLVPRAMFACCVL+GKI+VAGGFT+  KS S  E+
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKWRVEDYGWLP 240
           YD E D+W  +PDL QTH+S C G+V+ GKVH+++KG STVQ+ ++++L W V+DYGW  
Sbjct: 188 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDVKDYGWPQ 247

Query: 241 GPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVIGGVVRP 300
           GPM  V+D LYVM  GL+FKQ+G D   ++ + S+F  RIGMAM     E+ ++GGV+ P
Sbjct: 248 GPMVVVEDVLYVMSHGLVFKQEG-DTWKMVASASEFKRRIGMAMTSLSDEVLIVGGVIGP 307

Query: 301 VRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 347
            RL+ D+  LSDV  L +  ++P W  VAPM+RC GT+LGCT+L I
Sbjct: 308 DRLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 352

BLAST of MS018372 vs. TAIR 10
Match: AT1G16250.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 8.0e-37
Identity = 117/373 (31.37%), Postives = 161/373 (43.16%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCSY 63
           +I GLPD +ALRC+A++ +  H  LE VSR W+   R A+    +   G S   L V + 
Sbjct: 8   IIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLFVLTE 67

Query: 64  DPNNTWQLYDPLRDLWITLPELPSKRR--HLSHFGAASTSQYLFVLGGRSDAIDPLTGDH 123
              N W  YDP  D W  LP   + +   H S F     S  L V+GG   A    +  H
Sbjct: 68  RSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVCVSNCLLVIGG-CYAPSVSSFPH 127

Query: 124 DDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTST-SKSTSKTEI 183
              + T +V  FDP  + W M A M  PR  FAC  + GK+ VAGG   T S+     E+
Sbjct: 128 QKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGIPSAEV 187

Query: 184 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIM-----YKGQSTVQIFDTLELKWRVED 243
           YD   D W  LP  +      C G+   G  H++     +  Q++ ++F+  ++ W   +
Sbjct: 188 YDPVADRWEELP-AMPRPQMDCSGLSYRGCFHVLSDQVGFAEQNSSEVFNPRDMTWSTVE 247

Query: 244 YGWLPGPMA---AVQ----DSLYVMC---LGLIFKQD---------GRDRQVVIPAVSQF 303
             W P   A   AVQ    D +Y +      LI  +D         G    VV+P   + 
Sbjct: 248 DVW-PFSRAMQFAVQVMKNDRVYTIVDWGESLIKTRDTDEGEWYNVGSVPSVVLPNHPRE 307

Query: 304 LHRIGMAMIGFRGELYVIGGVVRPVRLDA----DLVKLSDVHVLNLRGEKPTWYCVAPMS 345
           L   G      R ELYVIGG V           D+V+L  V V N       W    PM 
Sbjct: 308 LEAFGYGFAALRNELYVIGGKVLKWEESGAGRFDIVRLPVVRVCNPLDRPLNWRETKPMC 367

BLAST of MS018372 vs. TAIR 10
Match: AT1G55270.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 3.8e-23
Identity = 78/258 (30.23%), Postives = 115/258 (44.57%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCSY 63
           L+ GLPD +A+ CL RVP   H KL LV + W         +  R+ +G SE+ + V   
Sbjct: 78  LLPGLPDDLAVACLIRVPRAEHRKLRLVCKRWYRLASGNFFYSQRKLLGMSEEWVYVFKR 137

Query: 64  DPNN--TWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQ-YLFVLGGRSDAIDPLTGD 123
           D +   +W  +DP+  LW  LP +P +      FG A  S  +L++ GG+    DPL G 
Sbjct: 138 DRDGKISWNTFDPISQLWQPLPPVPREYSEAVGFGCAVLSGCHLYLFGGK----DPLRG- 197

Query: 124 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKT-E 183
                S   V  ++  T  W     ML  R  F CCV+   + VAGG     + T ++ E
Sbjct: 198 -----SMRRVIFYNARTNKWHRAPDMLRKRHFFGCCVINNCLYVAGGECEGIQRTLRSAE 257

Query: 184 IYDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTV---QIFDTLELKWRVEDY 243
           +YD  K+ W  + D + T     IGVV   K  +   G   +   + +D     W     
Sbjct: 258 VYDPNKNRWSFIAD-MSTAMVPLIGVVYDKKWFLKGLGSHQLVMSEAYDPEVNSWSPVSD 317

Query: 244 GWLPG---PMAAVQDSLY 252
           G + G   P  ++   LY
Sbjct: 318 GMVAGWRNPCTSLNGRLY 324

BLAST of MS018372 vs. TAIR 10
Match: AT1G22040.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 2.2e-18
Identity = 61/223 (27.35%), Postives = 99/223 (44.39%), Query Frame = 0

Query: 4   LIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCVCS- 63
           LI  LPD ++++ LAR+P   +  + LVSR W++A  ++E++  R+E+G +E+ L V + 
Sbjct: 43  LIPSLPDELSIQILARLPRICYSSVRLVSRRWRSAVSTSEVYSLRKELGRTEEWLYVLTK 102

Query: 64  -YDPNNTWQLYDPLRDLWITLPELP------SKRRHL------------------SHFGA 123
            ++    W   DP+   W  LP +P        R+ L                  S  G 
Sbjct: 103 GHEDKLLWYALDPVSTKWQRLPPMPVVVYEEESRKSLSGLWNMITPSFNVGAIVRSFLGR 162

Query: 124 ASTSQYLFVLGGRSDAIDP---LTGDHDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFA 183
             +S+ +   G    A+D    + G    + + + VW FDP+  +W   + ML  RA   
Sbjct: 163 RDSSEQMPFCGCAIGAVDGGLYVIGGLSRSKTVSCVWRFDPILNSWSEVSSMLASRAYSK 222

Query: 184 CCVLEGKIIVAGGFTSTSKSTS---KTEIYDSEKDLWVPLPDL 195
             VL  K+ V GG        S     E+YD   D W  +P +
Sbjct: 223 TGVLNKKLYVVGGVDRGRGGLSPLQSAEVYDPSTDAWSEVPSM 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150165.18.4e-206100.00F-box/kelch-repeat protein SKIP30-like [Momordica charantia][more]
KAG6575922.14.7e-16479.19F-box/kelch-repeat protein SKIP30, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7014455.13.0e-16379.53F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022953500.14.0e-16378.32F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022953501.1 F-bo... [more]
XP_023547905.15.7e-16278.32F-box/kelch-repeat protein SKIP30-like [Cucurbita pepo subsp. pepo] >XP_02354790... [more]
Match NameE-valueIdentityDescription
Q9M1W71.5e-12558.67F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana OX=3702 GN=SKIP30 PE=1... [more]
Q0WW401.1e-3531.37F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g1625... [more]
Q93W935.4e-2230.23F-box/kelch-repeat protein At1g55270 OS=Arabidopsis thaliana OX=3702 GN=At1g5527... [more]
Q9FKJ08.1e-1829.29F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana OX=3702 GN=At5g6057... [more]
Q9LM553.1e-1727.35F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g2204... [more]
Match NameE-valueIdentityDescription
A0A6J1D7Q94.1e-206100.00F-box/kelch-repeat protein SKIP30-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1GPT21.9e-16378.32F-box/kelch-repeat protein SKIP30-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1JW983.4e-16076.88F-box/kelch-repeat protein SKIP30-like OS=Cucurbita maxima OX=3661 GN=LOC1114884... [more]
A0A0A0K7B11.8e-15375.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G387780 PE=4 SV=1[more]
A0A1S3BQL64.0e-15375.86F-box/kelch-repeat protein SKIP30-like OS=Cucumis melo OX=3656 GN=LOC103492650 P... [more]
Match NameE-valueIdentityDescription
AT3G63220.11.1e-12658.67Galactose oxidase/kelch repeat superfamily protein [more]
AT3G63220.21.1e-12658.67Galactose oxidase/kelch repeat superfamily protein [more]
AT1G16250.18.0e-3731.37Galactose oxidase/kelch repeat superfamily protein [more]
AT1G55270.13.8e-2330.23Galactose oxidase/kelch repeat superfamily protein [more]
AT1G22040.12.2e-1827.35Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 161..209
e-value: 1.5E-5
score: 34.5
coord: 103..160
e-value: 1.8E-5
score: 34.2
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 92..146
e-value: 1.1E-5
score: 24.9
coord: 149..194
e-value: 3.0E-11
score: 42.7
coord: 279..332
e-value: 2.5E-5
score: 23.8
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 57..342
e-value: 1.0E-35
score: 125.1
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 60..334
NoneNo IPR availablePANTHERPTHR24414F-BOX/KELCH-REPEAT PROTEIN SKIP4coord: 2..346
NoneNo IPR availablePANTHERPTHR24414:SF40F-BOX/KELCH-REPEAT PROTEIN SKIP30coord: 2..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018372.1MS018372.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding