MS018362 (gene) Bitter gourd (TR) v1

BLAST of MS018362 vs. NCBI nr
Match: XP_022150199.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 1459.1 bits (3776), Expect = 0.0e+00
Identity = 729/733 (99.45%), Postives = 730/733 (99.59%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEP RRIDKILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKL+NHYAFEHGDVETHFIERY
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIERY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVA LGKHN
Sbjct: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKHN 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS
Sbjct: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG
Sbjct: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 733

BLAST of MS018362 vs. NCBI nr
Match: XP_022150197.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 728/734 (99.18%), Postives = 730/734 (99.46%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEP RRIDKILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQS-IRVETGVEQGDAVSVHYD 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQS +RVETGVEQGDAVSVHYD
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSTVRVETGVEQGDAVSVHYD 420

Query: 421 PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIER 480
           PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKL+NHYAFEHGDVETHFIER
Sbjct: 421 PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIER 480

Query: 481 YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF 540
           YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF
Sbjct: 481 YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF 540

Query: 541 RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKH 600
           RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVA LGKH
Sbjct: 541 RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKH 600

Query: 601 NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA 660
           NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA
Sbjct: 601 NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA 660

Query: 661 SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA 720
           SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA
Sbjct: 661 SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA 720

Query: 721 GQQVSDGSILFSVK 734
           GQQVSDGSILFSVK
Sbjct: 721 GQQVSDGSILFSVK 734

BLAST of MS018362 vs. NCBI nr
Match: XP_022960107.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 1356.7 bits (3510), Expect = 0.0e+00
Identity = 675/733 (92.09%), Postives = 702/733 (95.77%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKL NKP LLRVRLFSQSNS  TEPARRI+KILIANRGEIACRI RTARRLGI
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNS-NTEPARRIEKILIANRGEIACRIARTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAASIIEAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQ C+D+ LTFIGPP SAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYY+AGTVEFIVDTISG+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI Q
Sbjct: 301 GYYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCL KFEVAGVPTNINFLLKLANH+AFE GDVETHFIE Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAK+AY+ A LNARLAAAC++ LE SKLN+KSSG GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNLVMAKQAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHH ARRT+E+EWEN YD SVSKPFAL ITYQQDGG LIETGENGSSAV VKV HLGKH 
Sbjct: 541 VHHCARRTIEYEWENPYDCSVSKPFALTITYQQDGGLLIETGENGSSAVEVKVTHLGKHK 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKDQTKHVHIWHGS HHHFKQKLG++LS+EDESQHKPGFEAAS
Sbjct: 601 FRVEVDGIIMEVRLAIYSKDQTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           +HP+GTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVAAG
Sbjct: 661 HHPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 732

BLAST of MS018362 vs. NCBI nr
Match: XP_023004327.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1356.3 bits (3509), Expect = 0.0e+00
Identity = 674/733 (91.95%), Postives = 702/733 (95.77%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKLPNKP LLRVRLFSQSNS  TEPARRI+KILIANRGEIACRI RTARRLGI
Sbjct: 1   MASIVFRRKLPNKPFLLRVRLFSQSNS-YTEPARRIEKILIANRGEIACRIARTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAA I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAATIIEAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQ C+D+ LTFIGPP SAIRHMGDKSASKRIMGAA VPLVPGYHGT QDIDTMKL
Sbjct: 121 ESADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTEQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYY+AGTVEFIVDTISG+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI Q
Sbjct: 301 GYYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPL GHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLFGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCL KFEVAGVPTNINFLLKLANH+AFE GDVETHFIE Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAKEAY+ A LNARLAAAC++ LE SKLN+KSSG GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNLVMAKEAYDAAWLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHH ARRT+E+EWEN YDSSVSKPFAL ITYQQDGG LIETGE+GSSAV VKV HLGK+ 
Sbjct: 541 VHHCARRTIEYEWENPYDSSVSKPFALTITYQQDGGLLIETGEHGSSAVEVKVTHLGKYK 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKDQTKH+HIWHGSHHHHFKQKLG++LS+EDESQHKPGFEAAS
Sbjct: 601 FRVEVDGIIMEVRLAIYSKDQTKHIHIWHGSHHHHFKQKLGVNLSEEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           +HP+GTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVAAG
Sbjct: 661 HHPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 732

BLAST of MS018362 vs. NCBI nr
Match: XP_023513665.1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 674/733 (91.95%), Postives = 701/733 (95.63%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKL NKP LLRVRLFSQSNS  TEPARRI+KILIANRGEIACRI RTARRLGI
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNS-NTEPARRIEKILIANRGEIACRIARTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNA +I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAVSIIEAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQ C+D+ LTFIGPP SAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYY+AGTVEFIVDTISG+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI Q
Sbjct: 301 GYYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCL KFEVAGVPTNINFLLKLANH+AFE GDVETHFIE Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAKEAY+ A LNARLAAAC++ LE SKLN+KSSG GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNLVMAKEAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHH ARRT+E+EWEN YDSSVSKPFAL ITYQ DGG LIETGENGSSAV VKV HLGKH 
Sbjct: 541 VHHCARRTIEYEWENPYDSSVSKPFALTITYQLDGGLLIETGENGSSAVEVKVTHLGKHK 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKD+TKHVHIWHGS HHHFKQKLG++LS+EDESQHKPGFEAAS
Sbjct: 601 FRVEVDGIIMEVRLAIYSKDRTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           +HP+GTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVAAG
Sbjct: 661 HHPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 732

BLAST of MS018362 vs. ExPASy Swiss-Prot
Match: Q42777 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Glycine max OX=3847 GN=MCCA PE=1 SV=2)

HSP 1 Score: 1060.1 bits (2740), Expect = 1.2e-308
Identity = 531/734 (72.34%), Postives = 612/734 (83.38%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MAS+   R+       +R R FS+  S+      RI+KIL+ANRGEIACRI RTARRLGI
Sbjct: 1   MASLALLRRTTLSHSHVRARAFSEGKSSN---RHRIEKILVANRGEIACRITRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAV+SDADRDSLHV +ADEA+ IGP PARLSYLN A+I+ AA R+GAQAIHPGYGFLS
Sbjct: 61  QTVAVYSDADRDSLHVATADEAIRIGPPPARLSYLNGASIVDAAIRSGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFA+LC++  LTFIGPP SAIR MGDKSASKRIMGAA VPLVPGYHG  QDI+ MKL
Sbjct: 121 ESADFAKLCEESGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGYDQDIEKMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EAD+IGYP+LIKPTHGGGGKGMRIVH+P+EF+++FL AQREAAASFG++TILLEKYIT+P
Sbjct: 181 EADRIGYPVLIKPTHGGGGKGMRIVHTPDEFVESFLAAQREAAASFGVNTILLEKYITRP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGD HGN+LHLYERDCSVQRRHQKIIEEAPAPN+  DFR+ LG AAVSAAKAV
Sbjct: 241 RHIEVQIFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISADFRAQLGVAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
            YY+AGTVEFIVDT+S EFYFMEMNTRLQVEHPVTEMIVGQDLVEWQI VANGE+LP+SQ
Sbjct: 301 NYYNAGTVEFIVDTVSDEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQILVANGEALPLSQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPL GHAFEARIYAENV KGFLPATG LHHY  VP S ++RVETGV++GD VS+HYDP
Sbjct: 361 SQVPLSGHAFEARIYAENVQKGFLPATGVLHHY-HVPVSSAVRVETGVKEGDKVSMHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKD L KF+VAG+PTN+NFL KLANH AF  G+VETHFI+ Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDSLSKFQVAGLPTNVNFLQKLANHRAFAIGNVETHFIDNY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKK-SSGNGIHSIWYSPPPF 540
           K+DLFVD +N V  KEAYE ARLNA L AAC++  EH  L +    G+ +  IWYS PPF
Sbjct: 481 KEDLFVDANNSVSVKEAYEAARLNASLVAACLIEKEHFILARNPPGGSSLLPIWYSSPPF 540

Query: 541 RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKH 600
           R+HH A+R ME EW+N+Y S  SK   L ITYQ DG +LIET +NGS  + VK  ++  +
Sbjct: 541 RIHHQAKRRMELEWDNEYGSGSSKIMKLTITYQPDGRYLIETEQNGSPVLEVKSTYVKDN 600

Query: 601 NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA 660
            FRVE  GVI +V +A+YSKDQ +H+HIW GS HH+F++KLG+ LS+++ESQHKP  E +
Sbjct: 601 YFRVEAAGVINDVNVAVYSKDQIRHIHIWQGSCHHYFREKLGLELSEDEESQHKPKVETS 660

Query: 661 SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA 720
           +N PQGTVVAPMAGLVVKVLV+N   V EGQ VLVLEAMKMEHVVKAP +G +HGL +  
Sbjct: 661 AN-PQGTVVAPMAGLVVKVLVENKTRVEEGQPVLVLEAMKMEHVVKAPSSGYVHGLQLMV 720

Query: 721 GQQVSDGSILFSVK 734
           G+QVSDGS+LFSVK
Sbjct: 721 GEQVSDGSVLFSVK 729

BLAST of MS018362 vs. ExPASy Swiss-Prot
Match: Q42523 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MCCA PE=1 SV=2)

HSP 1 Score: 1007.7 bits (2604), Expect = 7.2e-293
Identity = 513/728 (70.47%), Postives = 590/728 (81.04%), Query Frame = 0

Query: 7   RRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGIQTVAVF 66
           RR +  K   + VR  S S S   +  + I+KIL+ANRGEIACRI+RTA+RLGIQTVAV+
Sbjct: 10  RRNVRRKNHSMLVRYISGSASMKPK-EQCIEKILVANRGEIACRIMRTAKRLGIQTVAVY 69

Query: 67  SDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLSESADFA 126
           SDADRDSLHVKSADEAV IGP  ARLSYL+   I++AA+RTGAQAIHPGYGFLSES+DFA
Sbjct: 70  SDADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFA 129

Query: 127 QLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKLEADKIG 186
           QLC+D  LTFIGPP SAIR MGDKSASKRIMGAA VPLVPGYHG  QDID MK EA+KIG
Sbjct: 130 QLCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIG 189

Query: 187 YPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQPRHIEVQ 246
           YPI+IKPTHGGGGKGMRIV S  +F D+FLGAQREAAASFG++TILLEKYIT+PRHIEVQ
Sbjct: 190 YPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQ 249

Query: 247 IFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYSAG 306
           IFGD HGN+LHLYERDCSVQRRHQKIIEEAPAPN+ + FR++LGQAAVSAA+AVGYY+AG
Sbjct: 250 IFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAG 309

Query: 307 TVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQSQVPLL 366
           TVEFIVDT S +FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGE LP+SQS+VP+ 
Sbjct: 310 TVEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMS 369

Query: 367 GHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDPMIAKLV 426
           GHAFEARIYAENVPKGFLPATG L+HY PV  S S+RVETGVEQGD VS+HYDPMIAKLV
Sbjct: 370 GHAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLV 429

Query: 427 VWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERYKDDLFV 486
           VWG NR  ALVKLKDCL  F+VAGVPTNINFL KLA+H  F  G+VETHFIE +K DLF 
Sbjct: 430 VWGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFA 489

Query: 487 DPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFRVHHSAR 546
           D SN    + AY+  + +A L AACI  +EHS  N+ + G  + SIWYS PPFRVHH A+
Sbjct: 490 DESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGK-VPSIWYSNPPFRVHHEAK 549

Query: 547 RTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHNFRVEVD 606
           +T+E EW N+ + + S   +L + YQ DG +LIE G N S ++ ++V   GK +FRVE  
Sbjct: 550 QTIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEG-NDSPSLELRVTRAGKCDFRVEAA 609

Query: 607 GVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDES-QHKPGFEAASNHPQG 666
           G+ M V LA Y KD  KH+HIWHGS HH FKQK+GI  S+++E  QH+   E  S+HP G
Sbjct: 610 GLSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSE-TSSHPPG 669

Query: 667 TVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAGQQVSD 726
           T+VAPMAGLVVKVLV+N  +V +GQ +LVLEAMKMEHVVKAP +G I  L V AGQQVSD
Sbjct: 670 TIVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSD 729

Query: 727 GSILFSVK 734
           GS LF +K
Sbjct: 730 GSALFRIK 733

BLAST of MS018362 vs. ExPASy Swiss-Prot
Match: Q2QMG2 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=MCCA PE=2 SV=2)

HSP 1 Score: 973.8 bits (2516), Expect = 1.1e-282
Identity = 491/731 (67.17%), Postives = 580/731 (79.34%), Query Frame = 0

Query: 7   RRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGIQTVAVF 66
           RR       LL  RL S S+S+       ++K+L+ANRGEIACR++RTARRLGI TVAV+
Sbjct: 11  RRSRHGGASLLLARLLS-SSSSEAGGGGAVEKVLVANRGEIACRVMRTARRLGIPTVAVY 70

Query: 67  SDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLSESADFA 126
           SDADR +LHV++ADEAV +GP PAR SYLNA+AI+ AA RTGA+AIHPGYGFLSESADFA
Sbjct: 71  SDADRGALHVRAADEAVRLGPPPARESYLNASAIVDAALRTGAKAIHPGYGFLSESADFA 130

Query: 127 QLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKLEADKIG 186
           QLCK E LTFIGPP SAIR MGDKSASKRIMGAA VPLVPGYHG  QDI+ +KLEA+KIG
Sbjct: 131 QLCKAEGLTFIGPPPSAIRDMGDKSASKRIMGAAGVPLVPGYHGAEQDIELLKLEANKIG 190

Query: 187 YPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQPRHIEVQ 246
           YP+LIKPTHGGGGKGMRIV  P +F+D+ L AQREAAASFGI+T+L+EKYITQPRHIEVQ
Sbjct: 191 YPVLIKPTHGGGGKGMRIVQRPEDFVDSVLSAQREAAASFGINTLLVEKYITQPRHIEVQ 250

Query: 247 IFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYSAG 306
           IFGD HGN++HLYERDCS+QRRHQKIIEEAPAPNV   FRSH+G+AAVSAAKAVGYYSAG
Sbjct: 251 IFGDQHGNVIHLYERDCSLQRRHQKIIEEAPAPNVTAQFRSHIGEAAVSAAKAVGYYSAG 310

Query: 307 TVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQSQVPLL 366
           TVEFIVDT+SGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIR+ANGE LP+SQ QVPL 
Sbjct: 311 TVEFIVDTLSGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRIANGECLPLSQEQVPLN 370

Query: 367 GHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDPMIAKLV 426
           GHAFEARIYAENVP+GFLPATG+LHHY PVP++ ++RVETGVE+GD VS+HYDPMIAKLV
Sbjct: 371 GHAFEARIYAENVPRGFLPATGTLHHYRPVPSTATVRVETGVEEGDTVSMHYDPMIAKLV 430

Query: 427 VWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERYKDDLFV 486
           VWGE+R AALVKLK+ L  F++AG+PTN+ FL +LA H AFE G V+THFIERY++DL  
Sbjct: 431 VWGESRNAALVKLKNSLSNFQIAGLPTNVGFLQELAGHSAFEKGLVDTHFIERYQNDLLS 490

Query: 487 DPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIH----SIWYSPPPFRVH 546
             +  +    ++E   L A LAAACI   +H      SS   +H    S+WY+ PPFR+H
Sbjct: 491 TSTQALSG--SHEAEELGAILAAACICKKDH-----VSSEVSLHDKKLSMWYAHPPFRMH 550

Query: 547 HSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHNFR 606
           H A+R MEFE + +   S      L++TY+ DG + +ET +  S  + VKV   G H+FR
Sbjct: 551 HFAKRLMEFELDRELGGSSDDLLKLSVTYRSDGTYFVETEDGSSPGLDVKVDSRGDHDFR 610

Query: 607 VEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAASNH 666
           V+V G+  +V LA YSKD   H+HIWHG HHHH++Q L    S +D SQ     EA S H
Sbjct: 611 VDVGGLQTDVTLAFYSKDNCNHIHIWHGKHHHHYRQTLRAEQSPDDSSQPSASSEARS-H 670

Query: 667 PQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAGQQ 726
           P+G+V+APMAGLVVKVL+K+G  V EGQ V+V+EAMKMEHVVKAP AG + GL   AGQQ
Sbjct: 671 PKGSVLAPMAGLVVKVLLKDGARVEEGQPVMVMEAMKMEHVVKAPCAGYVEGLKATAGQQ 730

Query: 727 VSDGSILFSVK 734
           V D S+LF+VK
Sbjct: 731 VFDSSVLFTVK 732

BLAST of MS018362 vs. ExPASy Swiss-Prot
Match: Q99MR8 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Mccc1 PE=1 SV=2)

HSP 1 Score: 618.2 bits (1593), Expect = 1.2e-175
Identity = 339/696 (48.71%), Postives = 442/696 (63.51%), Query Frame = 0

Query: 30  TEPARRIDKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSP 89
           T P   I K+LIANRGEIACR+IRTA+++G+Q+VAV+S+ADR+S+HV  ADEA  IGP+P
Sbjct: 39  TTPGGSITKVLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAP 98

Query: 90  ARLSYLNAAAILQAASRTGAQAIHPGYGFLSESADFAQLCKDEALTFIGPPISAIRHMGD 149
           ++ SYL    I+Q A  + AQAIHPGYGFLSE+ +FA+LCK E + FIGPP SAIR MG 
Sbjct: 99  SQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSSAIRDMGI 158

Query: 150 KSASKRIMGAASVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPN 209
           KS SK IM AA VP+V GYHG  Q    ++  A KIGYP++IK   GGGGKGMRIV S  
Sbjct: 159 KSTSKSIMAAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKGMRIVRSER 218

Query: 210 EFIDAFLGAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRH 269
           EF +    A+REA  SF    +L+EK++  PRH+EVQ+FGD HGN ++L+ERDCSVQRRH
Sbjct: 219 EFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRH 278

Query: 270 QKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYSAGTVEFIVDTISGEFYFMEMNTRLQ 329
           QKIIEEAPAP +  + R  LG+AAV AAKAV Y  AGTVEFI+D+    FYFMEMNTRLQ
Sbjct: 279 QKIIEEAPAPGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDS-RHNFYFMEMNTRLQ 338

Query: 330 VEHPVTEMIVGQDLVEWQIRVANGESLPISQSQVPLLGHAFEARIYAENVPKGFLPATGS 389
           VEHPVTEMI G DLVEWQ+R+A GE +P+SQ ++PL GHAFEARIYAE+    F+P  G 
Sbjct: 339 VEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGP 398

Query: 390 LHHYCPVPASQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRAAALVKLKDCLKKFEVA 449
           L H     A  S R+ETGV QGD VSVHYDPMIAKLVVW  +R +AL KL+ CL ++ + 
Sbjct: 399 LVHLSTPSADMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYCLHQYNIV 458

Query: 450 GVPTNINFLLKLANHYAFEHGDVETHFIERYKDDLFVDPSNFVMAKEAYETARLNARLAA 509
           G+ +N++FLL+L+ H  FE G+V T FI ++  DL   PS+  +AKE+   A L   L  
Sbjct: 459 GLRSNVDFLLRLSGHPEFEAGNVHTDFIPQHHKDLL--PSHSTIAKESVCQAALGLIL-- 518

Query: 510 ACILHLEHSKLNKKSSGNGIHS-IWYSPPPFRVHHSARRTMEFEWENQYDSSVSKPFALA 569
                    K  + +S   +H+   +SP  F    S RR       N    S      +A
Sbjct: 519 ---------KEKEMTSAFKLHTQDQFSPFSF---SSGRRLNISYTRNMTLRSGKSDIVIA 578

Query: 570 ITYQQDGGFLIETGENGSSAVGVKVAHLGKHNFRVEVDGVIMEVRLAIYSKDQTKHVHIW 629
           +TY +DG + ++        +G   +  G    +  ++GV  + +  +   D T H+   
Sbjct: 579 VTYNRDGSYDMQIDNKSFRVLGDLSSEDGCTYLKSSINGVARKSKFILL--DNTVHLFSM 638

Query: 630 HGSHHHHFKQKLGIHLSDEDESQHKPGFEAASNHPQGTVVAPMAGLVVKVLVKNGDEVGE 689
            GS       ++GI +        K     ++   QG  +APM G + KV VK GD V  
Sbjct: 639 EGS------IEVGIPVP-------KYLSPVSAEGAQGGTIAPMTGTIEKVFVKAGDRVKA 698

Query: 690 GQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAGQQVS 725
           G S++V+ AMKMEH +KAP  G+I  +  + G Q +
Sbjct: 699 GDSLMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQAN 702

BLAST of MS018362 vs. ExPASy Swiss-Prot
Match: Q5I0C3 (Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Rattus norvegicus OX=10116 GN=Mccc1 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.5e-173
Identity = 344/723 (47.58%), Postives = 452/723 (62.52%), Query Frame = 0

Query: 7   RRKLPNKP-LLLRVRLFSQSNSTT---TEPARRIDKILIANRGEIACRIIRTARRLGIQT 66
           R +L   P LLL+ R +   + T    T P   I K+LIANRGEIACR+IRTAR++G+Q+
Sbjct: 12  RNQLRRVPILLLQPREWPWKHRTVKYGTTPGGSITKVLIANRGEIACRVIRTARKMGVQS 71

Query: 67  VAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLSES 126
           VAV+S+ADR+S+HV  ADEA  IGP+P++ SYL    I+Q A  + AQAIHPGYGFLSE+
Sbjct: 72  VAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSEN 131

Query: 127 ADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKLEA 186
            +FA+ CK E + FIGPP +AIR MG KS SK IM AA VP+V GYHG  Q  + +K  A
Sbjct: 132 MEFAEFCKQEGIIFIGPPSTAIRDMGIKSTSKSIMAAAGVPVVEGYHGNDQSDECLKEHA 191

Query: 187 DKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQPRH 246
            KIGYP++IK   GGGGKGMRI+ S  EF +    A+REA  SF    +L+EK++  PRH
Sbjct: 192 GKIGYPVMIKAIRGGGGKGMRIIRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRH 251

Query: 247 IEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGY 306
           +EVQ+FGD HGN ++L+ERDCSVQRRHQKIIEEAPAP +  + R  LG+AAV AAKAV Y
Sbjct: 252 VEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIDPEVRRRLGEAAVRAAKAVNY 311

Query: 307 YSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQSQ 366
             AGTVEFI+D+    FYFMEMNTRLQVEHPVTEMI G DLVEWQ+R+A GE +P+SQ +
Sbjct: 312 VGAGTVEFIMDS-KHNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEE 371

Query: 367 VPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDPMI 426
           +PL GHAFEARIYAE+    F+P  G L H    P   S R+ETGV QGD VSVHYDPMI
Sbjct: 372 IPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPPPDMSTRIETGVRQGDEVSVHYDPMI 431

Query: 427 AKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERYKD 486
           AKLVVW  +R +AL KL+  L ++ + G+ TN++FLL+L+ H  FE G+V T FI ++  
Sbjct: 432 AKLVVWASDRQSALSKLRYSLHQYNIVGLRTNVDFLLRLSGHSEFEAGNVHTDFIPQHHK 491

Query: 487 DLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHS-IWYSPPPFRV 546
           DL   P++  +AKE+   A L   L           K  + +S   +H+   +SP  F  
Sbjct: 492 DLL--PTHSTIAKESVCQAALGLIL-----------KEKEMTSAFKLHTQDQFSPFSF-- 551

Query: 547 HHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHNF 606
             S RR       N    S      +A+TY +DG + ++        +G      G    
Sbjct: 552 -SSGRRLNISYTRNMTLRSGKNDIIIAVTYNRDGSYDMQIENKLFRVLGDLSNEDGYTYL 611

Query: 607 RVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAASN 666
           +  V+GV  + +  +   D T ++    GS       ++GI +        K     ++ 
Sbjct: 612 KSSVNGVASKSKFILL--DNTIYLFSMEGS------IEVGIPVP-------KYLSPVSAE 671

Query: 667 HPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAGQ 725
             QG  +APM G + KV VK GD V  G +++V+ AMKMEH +KAP  G+I  +  + G 
Sbjct: 672 GTQGGTIAPMTGTIEKVFVKAGDRVKAGDALMVMIAMKMEHTIKAPKDGRIKKVFFSEGA 702

BLAST of MS018362 vs. ExPASy TrEMBL
Match: A0A6J1D9A1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018426 PE=4 SV=1)

HSP 1 Score: 1459.1 bits (3776), Expect = 0.0e+00
Identity = 729/733 (99.45%), Postives = 730/733 (99.59%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEP RRIDKILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKL+NHYAFEHGDVETHFIERY
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIERY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVA LGKHN
Sbjct: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKHN 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS
Sbjct: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG
Sbjct: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 733

BLAST of MS018362 vs. ExPASy TrEMBL
Match: A0A6J1DA31 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018426 PE=4 SV=1)

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 728/734 (99.18%), Postives = 730/734 (99.46%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEP RRIDKILIANRGEIACRIIRTARRLGI
Sbjct: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPTRRIDKILIANRGEIACRIIRTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ
Sbjct: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQS-IRVETGVEQGDAVSVHYD 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQS +RVETGVEQGDAVSVHYD
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSTVRVETGVEQGDAVSVHYD 420

Query: 421 PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIER 480
           PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKL+NHYAFEHGDVETHFIER
Sbjct: 421 PMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLSNHYAFEHGDVETHFIER 480

Query: 481 YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF 540
           YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF
Sbjct: 481 YKDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPF 540

Query: 541 RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKH 600
           RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVA LGKH
Sbjct: 541 RVHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAQLGKH 600

Query: 601 NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA 660
           NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA
Sbjct: 601 NFRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAA 660

Query: 661 SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA 720
           SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA
Sbjct: 661 SNHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAA 720

Query: 721 GQQVSDGSILFSVK 734
           GQQVSDGSILFSVK
Sbjct: 721 GQQVSDGSILFSVK 734

BLAST of MS018362 vs. ExPASy TrEMBL
Match: A0A6J1H6G1 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460955 PE=4 SV=1)

HSP 1 Score: 1356.7 bits (3510), Expect = 0.0e+00
Identity = 675/733 (92.09%), Postives = 702/733 (95.77%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKL NKP LLRVRLFSQSNS  TEPARRI+KILIANRGEIACRI RTARRLGI
Sbjct: 1   MASIVFRRKLTNKPFLLRVRLFSQSNS-NTEPARRIEKILIANRGEIACRIARTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAA+I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAASIIEAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQ C+D+ LTFIGPP SAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYY+AGTVEFIVDTISG+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI Q
Sbjct: 301 GYYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCL KFEVAGVPTNINFLLKLANH+AFE GDVETHFIE Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAK+AY+ A LNARLAAAC++ LE SKLN+KSSG GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNLVMAKQAYDAAGLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHH ARRT+E+EWEN YD SVSKPFAL ITYQQDGG LIETGENGSSAV VKV HLGKH 
Sbjct: 541 VHHCARRTIEYEWENPYDCSVSKPFALTITYQQDGGLLIETGENGSSAVEVKVTHLGKHK 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKDQTKHVHIWHGS HHHFKQKLG++LS+EDESQHKPGFEAAS
Sbjct: 601 FRVEVDGIIMEVRLAIYSKDQTKHVHIWHGSQHHHFKQKLGVNLSEEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           +HP+GTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVAAG
Sbjct: 661 HHPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 732

BLAST of MS018362 vs. ExPASy TrEMBL
Match: A0A6J1KQ45 (methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111497679 PE=4 SV=1)

HSP 1 Score: 1356.3 bits (3509), Expect = 0.0e+00
Identity = 674/733 (91.95%), Postives = 702/733 (95.77%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKLPNKP LLRVRLFSQSNS  TEPARRI+KILIANRGEIACRI RTARRLGI
Sbjct: 1   MASIVFRRKLPNKPFLLRVRLFSQSNS-YTEPARRIEKILIANRGEIACRIARTARRLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADR+SLHVK ADEAVHIGPSPARLSYLNAA I++AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRNSLHVKLADEAVHIGPSPARLSYLNAATIIEAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQ C+D+ LTFIGPP SAIRHMGDKSASKRIMGAA VPLVPGYHGT QDIDTMKL
Sbjct: 121 ESADFAQHCEDKGLTFIGPPTSAIRHMGDKSASKRIMGAAGVPLVPGYHGTEQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEF+DAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFVDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPN+LDDFRSHLGQAAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNILDDFRSHLGQAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYY+AGTVEFIVDTISG+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI Q
Sbjct: 301 GYYNAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPIIQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           SQVPL GHAFEARIYAENVPKGFLPATGSLHHYCPVP SQSIRVETGVEQGDAVSVHYDP
Sbjct: 361 SQVPLFGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSIRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENRAAALVKLKDCL KFEVAGVPTNINFLLKLANH+AFE GDVETHFIE Y
Sbjct: 421 MIAKLVVWGENRAAALVKLKDCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSN VMAKEAY+ A LNARLAAAC++ LE SKLN+KSSG GIHSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNLVMAKEAYDAAWLNARLAAACLVSLELSKLNEKSSGKGIHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHH ARRT+E+EWEN YDSSVSKPFAL ITYQQDGG LIETGE+GSSAV VKV HLGK+ 
Sbjct: 541 VHHCARRTIEYEWENPYDSSVSKPFALTITYQQDGGLLIETGEHGSSAVEVKVTHLGKYK 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDG+IMEVRLAIYSKDQTKH+HIWHGSHHHHFKQKLG++LS+EDESQHKPGFEAAS
Sbjct: 601 FRVEVDGIIMEVRLAIYSKDQTKHIHIWHGSHHHHFKQKLGVNLSEEDESQHKPGFEAAS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           +HP+GTVVAPMAGLVVKVLVKNGD+VGEGQSVLVLEAMKMEHVVKAP+AGQIHGLHVAAG
Sbjct: 661 HHPEGTVVAPMAGLVVKVLVKNGDQVGEGQSVLVLEAMKMEHVVKAPIAGQIHGLHVAAG 720

Query: 721 QQVSDGSILFSVK 734
           QQVSDGSILFSVK
Sbjct: 721 QQVSDGSILFSVK 732

BLAST of MS018362 vs. ExPASy TrEMBL
Match: A0A0A0K5L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G387180 PE=4 SV=1)

HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 661/733 (90.18%), Postives = 686/733 (93.59%), Query Frame = 0

Query: 1   MASIFFRRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGI 60
           MASI FRRKLP  P LLR RLFS SNS TT+P  RI+KILIANRGEIACRIIRTAR LGI
Sbjct: 1   MASIIFRRKLPTTPYLLRFRLFSHSNSNTTQPPPRINKILIANRGEIACRIIRTARSLGI 60

Query: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLS 120
           QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNA +I+ AASRTGAQAIHPGYGFLS
Sbjct: 61  QTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLNAPSIVDAASRTGAQAIHPGYGFLS 120

Query: 121 ESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKL 180
           ESADFAQLC DE LTFIGPPISAIRHMGDKSASKRIMGAA VPLVPGYHGTAQDIDTMKL
Sbjct: 121 ESADFAQLCGDEGLTFIGPPISAIRHMGDKSASKRIMGAAGVPLVPGYHGTAQDIDTMKL 180

Query: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240
           EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP
Sbjct: 181 EADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQP 240

Query: 241 RHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAV 300
           RHIEVQIFGDTHGNILHL ERDCSVQRRHQKIIEEAPAPNVLDDFRSHLG+AAVSAAKAV
Sbjct: 241 RHIEVQIFGDTHGNILHLNERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGEAAVSAAKAV 300

Query: 301 GYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQ 360
           GYYSAGTVEFIVDTISG+FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPI+Q
Sbjct: 301 GYYSAGTVEFIVDTISGQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPITQ 360

Query: 361 SQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDP 420
           +QVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVP SQS+RVETGVEQGDAVSVHYDP
Sbjct: 361 AQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPVPVSQSVRVETGVEQGDAVSVHYDP 420

Query: 421 MIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERY 480
           MIAKLVVWGENR+AAL KLK CL KFEVAGVPTNINFLLKLANH+AFE GDVETHFIE Y
Sbjct: 421 MIAKLVVWGENRSAALDKLKHCLTKFEVAGVPTNINFLLKLANHHAFERGDVETHFIEHY 480

Query: 481 KDDLFVDPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFR 540
           KDDLFVDPSN +MAKEAYE A  NA LAAACI+ LEHSKL +  SGN +HSIWYSPPPFR
Sbjct: 481 KDDLFVDPSNLLMAKEAYEAAGFNASLAAACIVSLEHSKLAENFSGNDLHSIWYSPPPFR 540

Query: 541 VHHSARRTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHN 600
           VHH AR T+EF WENQYDSS SKPF L ITYQQDGGFL+E+GE+GSSAV VKV+HLGKHN
Sbjct: 541 VHHCARCTVEFAWENQYDSSGSKPFPLTITYQQDGGFLVESGESGSSAVEVKVSHLGKHN 600

Query: 601 FRVEVDGVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDESQHKPGFEAAS 660
           FRVEVDGVIMEVRLAIYSKDQ KHVHIWHGS HHHFKQKLGI + DEDESQHKPGFEA S
Sbjct: 601 FRVEVDGVIMEVRLAIYSKDQIKHVHIWHGSRHHHFKQKLGIDVVDEDESQHKPGFEATS 660

Query: 661 NHPQGTVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAG 720
           NHPQGTVVAPMAGLVVKVL+KNGDEVGEGQ VLVLEAMKMEHVVKAP+AGQI+GLHVA G
Sbjct: 661 NHPQGTVVAPMAGLVVKVLMKNGDEVGEGQPVLVLEAMKMEHVVKAPIAGQIYGLHVAPG 720

Query: 721 QQVSDGSILFSVK 734
           QQV+DGS LFSVK
Sbjct: 721 QQVTDGSSLFSVK 733

BLAST of MS018362 vs. TAIR 10
Match: AT1G03090.2 (methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 (MCCA) )

HSP 1 Score: 1007.7 bits (2604), Expect = 5.1e-294
Identity = 513/728 (70.47%), Postives = 590/728 (81.04%), Query Frame = 0

Query: 7   RRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGIQTVAVF 66
           RR +  K   + VR  S S S   +  + I+KIL+ANRGEIACRI+RTA+RLGIQTVAV+
Sbjct: 10  RRNVRRKNHSMLVRYISGSASMKPK-EQCIEKILVANRGEIACRIMRTAKRLGIQTVAVY 69

Query: 67  SDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLSESADFA 126
           SDADRDSLHVKSADEAV IGP  ARLSYL+   I++AA+RTGAQAIHPGYGFLSES+DFA
Sbjct: 70  SDADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFA 129

Query: 127 QLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKLEADKIG 186
           QLC+D  LTFIGPP SAIR MGDKSASKRIMGAA VPLVPGYHG  QDID MK EA+KIG
Sbjct: 130 QLCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIG 189

Query: 187 YPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQPRHIEVQ 246
           YPI+IKPTHGGGGKGMRIV S  +F D+FLGAQREAAASFG++TILLEKYIT+PRHIEVQ
Sbjct: 190 YPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQ 249

Query: 247 IFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYSAG 306
           IFGD HGN+LHLYERDCSVQRRHQKIIEEAPAPN+ + FR++LGQAAVSAA+AVGYY+AG
Sbjct: 250 IFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAG 309

Query: 307 TVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQSQVPLL 366
           TVEFIVDT S +FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGE LP+SQS+VP+ 
Sbjct: 310 TVEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMS 369

Query: 367 GHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDPMIAKLV 426
           GHAFEARIYAENVPKGFLPATG L+HY PV  S S+RVETGVEQGD VS+HYDPMIAKLV
Sbjct: 370 GHAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLV 429

Query: 427 VWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERYKDDLFV 486
           VWG NR  ALVKLKDCL  F+VAGVPTNINFL KLA+H  F  G+VETHFIE +K DLF 
Sbjct: 430 VWGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFA 489

Query: 487 DPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFRVHHSAR 546
           D SN    + AY+  + +A L AACI  +EHS  N+ + G  + SIWYS PPFRVHH A+
Sbjct: 490 DESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGK-VPSIWYSNPPFRVHHEAK 549

Query: 547 RTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHNFRVEVD 606
           +T+E EW N+ + + S   +L + YQ DG +LIE G N S ++ ++V   GK +FRVE  
Sbjct: 550 QTIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEG-NDSPSLELRVTRAGKCDFRVEAA 609

Query: 607 GVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDES-QHKPGFEAASNHPQG 666
           G+ M V LA Y KD  KH+HIWHGS HH FKQK+GI  S+++E  QH+   E  S+HP G
Sbjct: 610 GLSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSE-TSSHPPG 669

Query: 667 TVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAGQQVSD 726
           T+VAPMAGLVVKVLV+N  +V +GQ +LVLEAMKMEHVVKAP +G I  L V AGQQVSD
Sbjct: 670 TIVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSD 729

Query: 727 GSILFSVK 734
           GS LF +K
Sbjct: 730 GSALFRIK 733

BLAST of MS018362 vs. TAIR 10
Match: AT1G03090.1 (methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 (MCCA) )

HSP 1 Score: 969.1 bits (2504), Expect = 2.0e-282
Identity = 500/728 (68.68%), Postives = 572/728 (78.57%), Query Frame = 0

Query: 7   RRKLPNKPLLLRVRLFSQSNSTTTEPARRIDKILIANRGEIACRIIRTARRLGIQTVAVF 66
           RR +  K   + VR  S S S   +  + I+KIL+ANRGEIACRI+RTA+RLGIQTVAV+
Sbjct: 10  RRNVRRKNHSMLVRYISGSASMKPK-EQCIEKILVANRGEIACRIMRTAKRLGIQTVAVY 69

Query: 67  SDADRDSLHVKSADEAVHIGPSPARLSYLNAAAILQAASRTGAQAIHPGYGFLSESADFA 126
           SDADRDSLHVKSADEAV IGP  ARLSYL+   I++AA+RTGAQAIHPGYGFLSES+DFA
Sbjct: 70  SDADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFA 129

Query: 127 QLCKDEALTFIGPPISAIRHMGDKSASKRIMGAASVPLVPGYHGTAQDIDTMKLEADKIG 186
           QLC+D  LTFIGPP SAIR MGDKSASKRIMGAA VPLVPGYHG  QDID MK EA+KIG
Sbjct: 130 QLCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIG 189

Query: 187 YPILIKPTHGGGGKGMRIVHSPNEFIDAFLGAQREAAASFGISTILLEKYITQPRHIEVQ 246
           YPI+IKPTHGGGGKGMRIV S  +F D+FLGAQREAAASFG++TILLEKYIT+PRHIEVQ
Sbjct: 190 YPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQ 249

Query: 247 IFGDTHGNILHLYERDCSVQRRHQKIIEEAPAPNVLDDFRSHLGQAAVSAAKAVGYYSAG 306
           IFGD HGN+LHLYERDCSVQRRHQKIIEEAPA                    AVGYY+AG
Sbjct: 250 IFGDKHGNVLHLYERDCSVQRRHQKIIEEAPA--------------------AVGYYNAG 309

Query: 307 TVEFIVDTISGEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGESLPISQSQVPLL 366
           TVEFIVDT S +FYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGE LP+SQS+VP+ 
Sbjct: 310 TVEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMS 369

Query: 367 GHAFEARIYAENVPKGFLPATGSLHHYCPVPASQSIRVETGVEQGDAVSVHYDPMIAKLV 426
           GHAFEARIYAENVPKGFLPATG L+HY PV  S S+RVETGVEQGD VS+HYDPMIAKLV
Sbjct: 370 GHAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLV 429

Query: 427 VWGENRAAALVKLKDCLKKFEVAGVPTNINFLLKLANHYAFEHGDVETHFIERYKDDLFV 486
           VWG NR  ALVKLKDCL  F+VAGVPTNINFL KLA+H  F  G+VETHFIE +K DLF 
Sbjct: 430 VWGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFA 489

Query: 487 DPSNFVMAKEAYETARLNARLAAACILHLEHSKLNKKSSGNGIHSIWYSPPPFRVHHSAR 546
           D SN    + AY+  + +A L AACI  +EHS  N+ + G  + SIWYS PPFRVHH A+
Sbjct: 490 DESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGK-VPSIWYSNPPFRVHHEAK 549

Query: 547 RTMEFEWENQYDSSVSKPFALAITYQQDGGFLIETGENGSSAVGVKVAHLGKHNFRVEVD 606
           +T+E EW N+ + + S   +L + YQ DG +LIE G N S ++ ++V   GK +FRVE  
Sbjct: 550 QTIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEG-NDSPSLELRVTRAGKCDFRVEAA 609

Query: 607 GVIMEVRLAIYSKDQTKHVHIWHGSHHHHFKQKLGIHLSDEDES-QHKPGFEAASNHPQG 666
           G+ M V LA Y KD  KH+HIWHGS HH FKQK+GI  S+++E  QH+   E  S+HP G
Sbjct: 610 GLSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSE-TSSHPPG 669

Query: 667 TVVAPMAGLVVKVLVKNGDEVGEGQSVLVLEAMKMEHVVKAPMAGQIHGLHVAAGQQVSD 726
           T+VAPMAGLVVKVLV+N  +V +GQ +LVLEAMKMEHVVKAP +G I  L V AGQQVSD
Sbjct: 670 TIVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSD 713

Query: 727 GSILFSVK 734
           GS LF +K
Sbjct: 730 GSALFRIK 713

BLAST of MS018362 vs. TAIR 10
Match: AT5G35360.1 (acetyl Co-enzyme a carboxylase biotin carboxylase subunit )

HSP 1 Score: 428.7 bits (1101), Expect = 9.8e-120
Identity = 218/460 (47.39%), Postives = 296/460 (64.35%), Query Frame = 0

Query: 37  DKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLN 96
           DKIL+ANRGEIA R+IRTA  +GI  VAV+S  D+D+LHVK ADEAV IG +P+  SYL 
Sbjct: 75  DKILVANRGEIAVRVIRTAHEMGIPCVAVYSTIDKDALHVKLADEAVCIGEAPSNQSYLV 134

Query: 97  AAAILQAASRTGAQAIHPGYGFLSESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRI 156
              +L AA   G   +HPGYGFLSE+A F ++C+D  + FIGP   +IR MGDK+ ++  
Sbjct: 135 IPNVLSAAISRGCTMLHPGYGFLSENALFVEMCRDHGINFIGPNPDSIRVMGDKATARET 194

Query: 157 MGAASVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFL 216
           M  A VP VPG  G  Q  +     A++IG+P++IK T GGGG+GMR+   P EF+    
Sbjct: 195 MKNAGVPTVPGSDGLLQSTEEAVRVANEIGFPVMIKATAGGGGRGMRLAKEPGEFVKLLQ 254

Query: 217 GAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEA 276
            A+ EAAA+FG     LEK++  PRHIE Q+  D  GN++H  ERDCS+QRR+QK++EEA
Sbjct: 255 QAKSEAAAAFGNDGCYLEKFVQNPRHIEFQVLADKFGNVVHFGERDCSIQRRNQKLLEEA 314

Query: 277 PAPNVLDDFRSHLGQAAVSAAKAVGYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTE 336
           P+P +  + R  +G AAV+AA ++GY   GTVEF++D   G FYFMEMNTR+QVEHPVTE
Sbjct: 315 PSPALTAELRKAMGDAAVAAAASIGYIGVGTVEFLLDE-RGSFYFMEMNTRIQVEHPVTE 374

Query: 337 MIVGQDLVEWQIRVANGESLPISQSQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPV 396
           MI   DL+E QIRVA GE L   Q  + L GH+ E RI AE+  KGF P  G +  Y P 
Sbjct: 375 MIYSVDLIEEQIRVAMGEKLRYKQEDIVLRGHSIECRINAEDPFKGFRPGPGRITSYLP- 434

Query: 397 PASQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNIN 456
                +R+++ V     V   YD ++ KL+VW   R  A+ ++K  L    + GVPT IN
Sbjct: 435 SGGPFVRMDSHVYSDYVVPPSYDSLLGKLIVWAPTREKAIERMKRALNDTIITGVPTTIN 494

Query: 457 FLLKLANHYAFEHGDVETHFIERYKDDLFVDPSNFVMAKE 497
           +   + +   F++G V+T FI +++++L  +P   V  K+
Sbjct: 495 YHKLILDVEDFKNGKVDTAFIVKHEEEL-AEPQEIVAVKD 531

BLAST of MS018362 vs. TAIR 10
Match: AT5G35360.3 (acetyl Co-enzyme a carboxylase biotin carboxylase subunit )

HSP 1 Score: 425.6 bits (1093), Expect = 8.3e-119
Identity = 215/448 (47.99%), Postives = 291/448 (64.96%), Query Frame = 0

Query: 37  DKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLN 96
           DKIL+ANRGEIA R+IRTA  +GI  VAV+S  D+D+LHVK ADEAV IG +P+  SYL 
Sbjct: 75  DKILVANRGEIAVRVIRTAHEMGIPCVAVYSTIDKDALHVKLADEAVCIGEAPSNQSYLV 134

Query: 97  AAAILQAASRTGAQAIHPGYGFLSESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRI 156
              +L AA   G   +HPGYGFLSE+A F ++C+D  + FIGP   +IR MGDK+ ++  
Sbjct: 135 IPNVLSAAISRGCTMLHPGYGFLSENALFVEMCRDHGINFIGPNPDSIRVMGDKATARET 194

Query: 157 MGAASVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFL 216
           M  A VP VPG  G  Q  +     A++IG+P++IK T GGGG+GMR+   P EF+    
Sbjct: 195 MKNAGVPTVPGSDGLLQSTEEAVRVANEIGFPVMIKATAGGGGRGMRLAKEPGEFVKLLQ 254

Query: 217 GAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEA 276
            A+ EAAA+FG     LEK++  PRHIE Q+  D  GN++H  ERDCS+QRR+QK++EEA
Sbjct: 255 QAKSEAAAAFGNDGCYLEKFVQNPRHIEFQVLADKFGNVVHFGERDCSIQRRNQKLLEEA 314

Query: 277 PAPNVLDDFRSHLGQAAVSAAKAVGYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTE 336
           P+P +  + R  +G AAV+AA ++GY   GTVEF++D   G FYFMEMNTR+QVEHPVTE
Sbjct: 315 PSPALTAELRKAMGDAAVAAAASIGYIGVGTVEFLLDE-RGSFYFMEMNTRIQVEHPVTE 374

Query: 337 MIVGQDLVEWQIRVANGESLPISQSQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPV 396
           MI   DL+E QIRVA GE L   Q  + L GH+ E RI AE+  KGF P  G +  Y P 
Sbjct: 375 MIYSVDLIEEQIRVAMGEKLRYKQEDIVLRGHSIECRINAEDPFKGFRPGPGRITSYLP- 434

Query: 397 PASQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTNIN 456
                +R+++ V     V   YD ++ KL+VW   R  A+ ++K  L    + GVPT IN
Sbjct: 435 SGGPFVRMDSHVYSDYVVPPSYDSLLGKLIVWAPTREKAIERMKRALNDTIITGVPTTIN 494

Query: 457 FLLKLANHYAFEHGDVETHFIERYKDDL 485
           +   + +   F++G V+T FI +++++L
Sbjct: 495 YHKLILDVEDFKNGKVDTAFIVKHEEEL 520

BLAST of MS018362 vs. TAIR 10
Match: AT5G35360.2 (acetyl Co-enzyme a carboxylase biotin carboxylase subunit )

HSP 1 Score: 405.2 bits (1040), Expect = 1.2e-112
Identity = 204/418 (48.80%), Postives = 271/418 (64.83%), Query Frame = 0

Query: 37  DKILIANRGEIACRIIRTARRLGIQTVAVFSDADRDSLHVKSADEAVHIGPSPARLSYLN 96
           DKIL+ANRGEIA R+IRTA  +GI  VAV+S  D+D+LHVK ADEAV IG +P+  SYL 
Sbjct: 75  DKILVANRGEIAVRVIRTAHEMGIPCVAVYSTIDKDALHVKLADEAVCIGEAPSNQSYLV 134

Query: 97  AAAILQAASRTGAQAIHPGYGFLSESADFAQLCKDEALTFIGPPISAIRHMGDKSASKRI 156
              +L AA   G   +HPGYGFLSE+A F ++C+D  + FIGP   +IR MGDK+ ++  
Sbjct: 135 IPNVLSAAISRGCTMLHPGYGFLSENALFVEMCRDHGINFIGPNPDSIRVMGDKATARET 194

Query: 157 MGAASVPLVPGYHGTAQDIDTMKLEADKIGYPILIKPTHGGGGKGMRIVHSPNEFIDAFL 216
           M  A VP VPG  G  Q  +     A++IG+P++IK T GGGG+GMR+   P EF+    
Sbjct: 195 MKNAGVPTVPGSDGLLQSTEEAVRVANEIGFPVMIKATAGGGGRGMRLAKEPGEFVKLLQ 254

Query: 217 GAQREAAASFGISTILLEKYITQPRHIEVQIFGDTHGNILHLYERDCSVQRRHQKIIEEA 276
            A+ EAAA+FG     LEK++  PRHIE Q+  D  GN++H  ERDCS+QRR+QK++EEA
Sbjct: 255 QAKSEAAAAFGNDGCYLEKFVQNPRHIEFQVLADKFGNVVHFGERDCSIQRRNQKLLEEA 314

Query: 277 PAPNVLDDFRSHLGQAAVSAAKAVGYYSAGTVEFIVDTISGEFYFMEMNTRLQVEHPVTE 336
           P+P +  + R  +G AAV+AA ++GY   GTVEF++D   G FYFMEMNTR+QVEHPVTE
Sbjct: 315 PSPALTAELRKAMGDAAVAAAASIGYIGVGTVEFLLDE-RGSFYFMEMNTRIQVEHPVTE 374

Query: 337 MIVGQDLVEWQIRVANGESLPISQSQVPLLGHAFEARIYAENVPKGFLPATGSLHHYCPV 396
           MI   DL+E QIRVA GE L   Q  + L GH+ E RI AE+  KGF P  G +  Y P 
Sbjct: 375 MIYSVDLIEEQIRVAMGEKLRYKQEDIVLRGHSIECRINAEDPFKGFRPGPGRITSYLP- 434

Query: 397 PASQSIRVETGVEQGDAVSVHYDPMIAKLVVWGENRAAALVKLKDCLKKFEVAGVPTN 455
                +R+++ V     V   YD ++ KL+VW   R  A+ ++K  L    + G+ +N
Sbjct: 435 SGGPFVRMDSHVYSDYVVPPSYDSLLGKLIVWAPTREKAIERMKRALNDTIITGMGSN 490