MS018143 (gene) Bitter gourd (TR) v1

Overview


Name	MS018143
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cupin_3 domain-containing protein
Location	scaffold166: 52186 .. 52709 (-)
RNA-Seq Expression	MS018143
Synteny	MS018143

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCATGGCCACCGAGTTGTTTGGAGTGAAGATCGAAAGAAATGTTCCACAGTCCAAACTCAAGGAGCTCGATGTTTACACATGGCCCAAGTATGTCTTTTTTTATTTGATTTTCACGACTTTTTAGTTTGAGATATATACCTTAATCAGTTAATTTATATCCAATCATCTTCTTCCTGCGAAAACAATTTCGTTTCTTAAATCTCTCATTTTGAAACTCTCTCTTCCTTTTGCCGCATTTGTTATTTTTTTCTCTGTTTAACCACAGGAATGAAAATTGATGGGAATAATTAACGTGGGGTGTTGGTGATTGTTGTAATTACAAATAGGTGGAGTTGTGGTCCAAGCAAATTCGATTGGACATTCAGTGCGATGGAGACAGTGTATCTGATAGAGGGTAAAGCCAAAATCAAGATCGAAGAGCACAATGAGATATTTGAAATTGGGGCTGGAGATATGGCTATTTTTCCCTCTGGAATGAAGATTAATTTGGATGTCATAGAAGCCATAGACAAGCATTATAAG

mRNA sequence

ACCATGGCCACCGAGTTGTTTGGAGTGAAGATCGAAAGAAATGTTCCACAGTCCAAACTCAAGGAGCTCGATGTTTACACATGGCCCAAGTGGAGTTGTGGTCCAAGCAAATTCGATTGGACATTCAGTGCGATGGAGACAGTGTATCTGATAGAGGGTAAAGCCAAAATCAAGATCGAAGAGCACAATGAGATATTTGAAATTGGGGCTGGAGATATGGCTATTTTTCCCTCTGGAATGAAGATTAATTTGGATGTCATAGAAGCCATAGACAAGCATTATAAG

Coding sequence (CDS)

ACCATGGCCACCGAGTTGTTTGGAGTGAAGATCGAAAGAAATGTTCCACAGTCCAAACTCAAGGAGCTCGATGTTTACACATGGCCCAAGTGGAGTTGTGGTCCAAGCAAATTCGATTGGACATTCAGTGCGATGGAGACAGTGTATCTGATAGAGGGTAAAGCCAAAATCAAGATCGAAGAGCACAATGAGATATTTGAAATTGGGGCTGGAGATATGGCTATTTTTCCCTCTGGAATGAAGATTAATTTGGATGTCATAGAAGCCATAGACAAGCATTATAAG

Protein sequence

TMATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEEHNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHYK

Homology

[Close]

BLAST of MS018143 vs. NCBI nr
Match: XP_022156027.1 (uncharacterized protein LOC111022998 [Momordica charantia])

HSP 1 Score: 156.4 bits (394), Expect = 1.2e-34
Identity = 70/76 (92.11%), Postives = 74/76 (97.37%), Query Frame = 0


Query: 2  MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
          MATELFGV+IERNVPQ+KLKELDVYTWPKWSCGPSKFDWTFSAMETVY +EGKAKIKIEE
Sbjct: 1  MATELFGVRIERNVPQAKLKELDVYTWPKWSCGPSKFDWTFSAMETVYQLEGKAKIKIEE 60

Query: 62 HNEIFEIGAGDMAIFP 78
          HNE FEIGAGDMA+FP
Sbjct: 61 HNETFEIGAGDMAVFP 76

[Close]

BLAST of MS018143 vs. NCBI nr
Match: XP_031286750.1 (uncharacterized protein LOC116145430 [Pistacia vera])

HSP 1 Score: 141.4 bits (355), Expect = 4.1e-30
Identity = 61/94 (64.89%), Postives = 73/94 (77.66%), Query Frame = 0


Query: 1   TMATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIE 60
           T  TE+FGVKIE+N PQSKL ELDV +WPKW C PSKF WTF A ET+YL+EGK ++ +E
Sbjct: 113 TTTTEIFGVKIEKNPPQSKLDELDVSSWPKWECPPSKFPWTFKATETMYLLEGKVQVYVE 172

Query: 61  EHNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
            H   FEIGAGD+ IFP GM I  DV+EA++KHY
Sbjct: 173 GHEGSFEIGAGDLVIFPKGMNITWDVLEAVNKHY 206

[Close]

BLAST of MS018143 vs. NCBI nr
Match: CAB4279449.1 (unnamed protein product [Prunus armeniaca] >CAB4309909.1 unnamed protein product [Prunus armeniaca])

HSP 1 Score: 136.7 bits (343), Expect = 1.0e-28
Identity = 58/93 (62.37%), Postives = 73/93 (78.49%), Query Frame = 0


Query: 2   MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
           MAT  FGVK+E+N  QSKL EL V  WPKWSCGPSKF W+F+A ET+YL+EGK  ++++ 
Sbjct: 47  MATNAFGVKVEKNPAQSKLTELGVTAWPKWSCGPSKFPWSFTATETMYLLEGKVIVQVDG 106

Query: 62  HNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           H   F IGAGD+A+FP GMK+  DVIEA++KHY
Sbjct: 107 HEGSFTIGAGDLAVFPKGMKVTWDVIEAVNKHY 139

[Close]

BLAST of MS018143 vs. NCBI nr
Match: XP_008238399.1 (PREDICTED: uncharacterized protein LOC103337036 [Prunus mume])

HSP 1 Score: 136.7 bits (343), Expect = 1.0e-28
Identity = 58/93 (62.37%), Postives = 73/93 (78.49%), Query Frame = 0


Query: 2   MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
           MAT  FGVK+E+N  QSKL EL V  WPKWSCGPSKF W+F+A ET+YL+EGK  ++++ 
Sbjct: 47  MATNAFGVKVEKNPAQSKLTELGVTAWPKWSCGPSKFPWSFTATETMYLLEGKVIVQVDG 106

Query: 62  HNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           H   F IGAGD+A+FP GMK+  DVIEA++KHY
Sbjct: 107 HEGSFTIGAGDLAVFPKGMKVTWDVIEAVNKHY 139

[Close]

BLAST of MS018143 vs. NCBI nr
Match: KAF3951790.1 (hypothetical protein CMV_022594 [Castanea mollissima])

HSP 1 Score: 136.7 bits (343), Expect = 1.0e-28
Identity = 56/94 (59.57%), Postives = 76/94 (80.85%), Query Frame = 0


Query: 1   TMATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIE 60
           +MATE FGVKI++N P+SKL EL V TWPKW CGPSKF W F+A ET++L++GK K+ ++
Sbjct: 44  SMATEKFGVKIQKNPPESKLTELGVRTWPKWGCGPSKFPWEFTATETMFLLDGKVKVYVD 103

Query: 61  EHNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
            ++E FEIGAGD+ +FP GMK+  DV +A++KHY
Sbjct: 104 GYDESFEIGAGDLVVFPKGMKVTWDVTKAVNKHY 137

[Close]

BLAST of MS018143 vs. ExPASy TrEMBL
Match: A0A6J1DQY7 (uncharacterized protein LOC111022998 OS=Momordica charantia OX=3673 GN=LOC111022998 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 6.0e-35
Identity = 70/76 (92.11%), Postives = 74/76 (97.37%), Query Frame = 0


Query: 2  MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
          MATELFGV+IERNVPQ+KLKELDVYTWPKWSCGPSKFDWTFSAMETVY +EGKAKIKIEE
Sbjct: 1  MATELFGVRIERNVPQAKLKELDVYTWPKWSCGPSKFDWTFSAMETVYQLEGKAKIKIEE 60

Query: 62 HNEIFEIGAGDMAIFP 78
          HNE FEIGAGDMA+FP
Sbjct: 61 HNETFEIGAGDMAVFP 76

[Close]

BLAST of MS018143 vs. ExPASy TrEMBL
Match: A0A6J5USB1 (Cupin_3 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS31714 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.9e-29
Identity = 58/93 (62.37%), Postives = 73/93 (78.49%), Query Frame = 0


Query: 2   MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
           MAT  FGVK+E+N  QSKL EL V  WPKWSCGPSKF W+F+A ET+YL+EGK  ++++ 
Sbjct: 47  MATNAFGVKVEKNPAQSKLTELGVTAWPKWSCGPSKFPWSFTATETMYLLEGKVIVQVDG 106

Query: 62  HNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           H   F IGAGD+A+FP GMK+  DVIEA++KHY
Sbjct: 107 HEGSFTIGAGDLAVFPKGMKVTWDVIEAVNKHY 139

[Close]

BLAST of MS018143 vs. ExPASy TrEMBL
Match: A0A6P5S959 (uncharacterized protein LOC110756390 OS=Prunus avium OX=42229 GN=LOC110756390 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.4e-29
Identity = 58/93 (62.37%), Postives = 73/93 (78.49%), Query Frame = 0


Query: 2   MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
           MAT  FGVK+E+N  QSKL EL V TWPKWSCGPSKF W+F+A ET+YL+EGK  ++++ 
Sbjct: 47  MATNAFGVKVEKNPAQSKLTELGVTTWPKWSCGPSKFPWSFTATETMYLLEGKVIVQVDG 106

Query: 62  HNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           H   F IGAGD+A+FP  MK+  DVIEA++KHY
Sbjct: 107 HEGSFTIGAGDLAVFPKDMKVTWDVIEAVNKHY 139

[Close]

BLAST of MS018143 vs. ExPASy TrEMBL
Match: M5WCB9 (Cupin_3 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_5G060300 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 57/93 (61.29%), Postives = 72/93 (77.42%), Query Frame = 0


Query: 2   MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
           MAT  FGVK+E N  QSKL EL V  WPKWSCGPSKF W+F+A ET+YL+EGK  ++++ 
Sbjct: 47  MATNAFGVKVENNPAQSKLTELGVTAWPKWSCGPSKFPWSFTATETMYLLEGKVIVQVDG 106

Query: 62  HNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           H   F IGAGD+A+FP GMK+  DV+EA++KHY
Sbjct: 107 HEGSFTIGAGDLAVFPKGMKVTWDVLEAVNKHY 139

[Close]

BLAST of MS018143 vs. ExPASy TrEMBL
Match: A0A059BYF9 (Cupin_3 domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F04106 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 58/93 (62.37%), Postives = 72/93 (77.42%), Query Frame = 0


Query: 2   MATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEE 61
           + TE+ GVKIE+N PQSKL EL V  WPKW CGPSKF WTF++ ET+YL+EGK  + I+ 
Sbjct: 50  LVTEIHGVKIEKNPPQSKLDELGVADWPKWGCGPSKFPWTFTSTETMYLLEGKVTVYIDG 109

Query: 62  HNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           H+  FEIG GD+ IFP GMKI  DV+EA++KHY
Sbjct: 110 HDGSFEIGGGDLVIFPKGMKIVWDVVEAVNKHY 142

[Close]

BLAST of MS018143 vs. TAIR 10
Match: AT4G10300.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.9e-25
Identity = 49/93 (52.69%), Postives = 63/93 (67.74%), Query Frame = 0


Query: 3   ATELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEEH 62
           +TE  G+ IE+N P+SKL +L V +WPKW C PSKF WT+SA ET YL++GK K+     
Sbjct: 40  STEKLGITIEKNPPESKLTQLGVRSWPKWGCPPSKFPWTYSAKETCYLLQGKVKVYPNGS 99

Query: 63  NEIFEIGAGDMAIFPSGMKINLDVIEAIDKHYK 96
           +E  EI AGD  +FP GM    DV  A+DKHY+
Sbjct: 100 DEGVEIEAGDFVVFPKGMSCTWDVSVAVDKHYQ 132

[Close]

BLAST of MS018143 vs. TAIR 10
Match: AT4G10280.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 5.8e-22
Identity = 44/94 (46.81%), Postives = 59/94 (62.77%), Query Frame = 0


Query: 4   TELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKI---E 63
           TE+ GVKI R    +KL +L V +WPKW   PSKF W F   ET+Y +EGK K+ +   +
Sbjct: 43  TEIHGVKILRQASDTKLAQLGVASWPKWEGAPSKFPWEFKKTETIYFMEGKVKVNVDGYD 102

Query: 64  EHNEIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
           E  E FEIG GD+ +FP  MK+  ++ EA+ K Y
Sbjct: 103 EEEETFEIGKGDVVVFPKDMKVVWEITEAVKKQY 136

[Close]

BLAST of MS018143 vs. TAIR 10
Match: AT4G10290.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 4.1e-20
Identity = 42/94 (44.68%), Postives = 57/94 (60.64%), Query Frame = 0


Query: 4   TELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEEHN 63
           TE+ GVK+ R    +KL EL V +W  W   P KF W F   ET+Y +EGK K+K+E+H+
Sbjct: 15  TEIHGVKVMRQTSDAKLAELGVTSWQLWESMPRKFPWKFKKTETMYFVEGKLKVKVEDHH 74

Query: 64  ---EIFEIGAGDMAIFPSGMKINLDVIEAIDKHY 95
              E  E  AGD+ +FP  M + +DVIE + K Y
Sbjct: 75  KEGEALEFVAGDLVVFPQDMNVFVDVIEDVKKRY 108

[Close]

BLAST of MS018143 vs. TAIR 10
Match: AT3G04300.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.1e-17
Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 9  VKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEEHNEIFEI 68
          + IE N    +L +L V +WPKWSC P K+   F   ET YL++GK K+  +  +E  E 
Sbjct: 3  IVIENNPSSRRLSDLGVMSWPKWSCQPGKYALVFEERETCYLVKGKVKVYPKGSSEFVEF 62

Query: 69 GAGDMAIFPSGMKINLDVIEAIDKHYK 96
          GAGD+   P G+    DV   IDKHYK
Sbjct: 63 GAGDLVTIPKGLSCTWDVSLFIDKHYK 89

[Close]

BLAST of MS018143 vs. TAIR 10
Match: AT2G32650.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 3.9e-10
Identity = 26/80 (32.50%), Postives = 43/80 (53.75%), Query Frame = 0


Query: 5   ELFGVKIERNVPQSKLKELDVYTWPKWSCGPSKFDWTFSAMETVYLIEGKAKIKIEEHNE 64
           EL+ VK+ER V Q +L+EL V  W  W  G  K  W +   + VY+ +G+ ++  E    
Sbjct: 42  ELYNVKVERKVSQKRLEELGVSRWSVWKTGKCKLPWDWQVDQLVYIEQGEVRVVPEGSKR 101

Query: 65  IFEIGAGDMAIFPSGMKINL 85
             +  AGD+  +P  ++ +L
Sbjct: 102 YMQFLAGDLVRYPKWLEADL 121

The following BLAST results are available for this feature:

BLAST of MS018143 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156027.1	1.2e-34	92.11	uncharacterized protein LOC111022998 [Momordica charantia]	[more]
XP_031286750.1	4.1e-30	64.89	uncharacterized protein LOC116145430 [Pistacia vera]	[more]
CAB4279449.1	1.0e-28	62.37	unnamed protein product [Prunus armeniaca] >CAB4309909.1 unnamed protein product...	[more]
XP_008238399.1	1.0e-28	62.37	PREDICTED: uncharacterized protein LOC103337036 [Prunus mume]	[more]
KAF3951790.1	1.0e-28	59.57	hypothetical protein CMV_022594 [Castanea mollissima]	[more]

BLAST of MS018143 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS018143 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DQY7	6.0e-35	92.11	uncharacterized protein LOC111022998 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J5USB1	4.9e-29	62.37	Cupin_3 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS31...	[more]
A0A6P5S959	8.4e-29	62.37	uncharacterized protein LOC110756390 OS=Prunus avium OX=42229 GN=LOC110756390 PE...	[more]
M5WCB9	1.4e-28	61.29	Cupin_3 domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_5G060300 PE...	[more]
A0A059BYF9	1.4e-28	62.37	Cupin_3 domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F041...	[more]

BLAST of MS018143 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10300.1	1.9e-25	52.69	RmlC-like cupins superfamily protein	[more]
AT4G10280.1	5.8e-22	46.81	RmlC-like cupins superfamily protein	[more]
AT4G10290.1	4.1e-20	44.68	RmlC-like cupins superfamily protein	[more]
AT3G04300.1	1.1e-17	43.68	RmlC-like cupins superfamily protein	[more]
AT2G32650.1	3.9e-10	32.50	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008579	(S)-ureidoglycine aminohydrolase, cupin-3 domain	PFAM	PF05899	Cupin_3	coord: 20..94 e-value: 1.3E-20 score: 72.8
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 1..95 e-value: 8.1E-29 score: 101.2
None	No IPR available	PANTHER	PTHR33271:SF1	RMLC-LIKE JELLY ROLL PROTEIN-RELATED	coord: 2..94
None	No IPR available	PANTHER	PTHR33271	OS04G0445200 PROTEIN	coord: 2..94
None	No IPR available	CDD	cd02227	cupin_TM1112-like	coord: 27..95 e-value: 2.90672E-23 score: 82.6284
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 13..94

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018143.1	MS018143.1	mRNA