MS018013 (gene) Bitter gourd (TR) v1

Overview
NameMS018013
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiondehydration-responsive element-binding protein 1E-like
Locationscaffold373: 4668707 .. 4669381 (+)
RNA-Seq ExpressionMS018013
SyntenyMS018013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATGTTCTTCGGTCAATTTTCCGATCCTCTTCCTTACGGCGACAAGTCGGAATCGTCGACGTGGTCGGACGCCGGCACGCCGGTTACTCACCCGGCGGCGCACTCCGATGAGGAAGTGATACTGGCGGCGAGCCGGCCGAAGCGGCGGGCCGGGCGGAGGGTTTTTAAGGAGACGAGGCACCCGGTTTACCGGGGGGTCCGGCGGAGGAACAACGATAAATGGGTGTGCGAGTTGCGTGAGCCGAATAAGAAGTCTAGGATCTGGCTCGGGACGTATCCGACGGCGGAGATGGCAGCGCGTGCGCACGACGTCGCGGCACTCGCGTTGAGAGGCAAGTCGGCTTGCCTCAACTTTGCTGACTCGGCGTGGCGATTTCCCATTCCGGAGTCTACTGATCCGAGTGTCATCCGGGACGCGGCGGCTCGGGCGGCCAATGAGTGCAAGGATGAAGAGGACGACGCCGTTTTGGGAGGCGGAGAAGGAGAACACCCCGCCAGGGGGGAGCCAGCTCCTCCGGCTTACGTGGACGATGAGGCCTTGTCCAACATGCCGATGCTGCTTGCCAGCATGGCGGAGGGACTTCTTCTCTCTCCTCCTTCTTTTTGCATGCATGACGACGTGGAGTGGGGTGACGTGGACATGAGTGATGACATGTCGCTGTGGAGCTTT

mRNA sequence

ATGGATATGTTCTTCGGTCAATTTTCCGATCCTCTTCCTTACGGCGACAAGTCGGAATCGTCGACGTGGTCGGACGCCGGCACGCCGGTTACTCACCCGGCGGCGCACTCCGATGAGGAAGTGATACTGGCGGCGAGCCGGCCGAAGCGGCGGGCCGGGCGGAGGGTTTTTAAGGAGACGAGGCACCCGGTTTACCGGGGGGTCCGGCGGAGGAACAACGATAAATGGGTGTGCGAGTTGCGTGAGCCGAATAAGAAGTCTAGGATCTGGCTCGGGACGTATCCGACGGCGGAGATGGCAGCGCGTGCGCACGACGTCGCGGCACTCGCGTTGAGAGGCAAGTCGGCTTGCCTCAACTTTGCTGACTCGGCGTGGCGATTTCCCATTCCGGAGTCTACTGATCCGAGTGTCATCCGGGACGCGGCGGCTCGGGCGGCCAATGAGTGCAAGGATGAAGAGGACGACGCCGTTTTGGGAGGCGGAGAAGGAGAACACCCCGCCAGGGGGGAGCCAGCTCCTCCGGCTTACGTGGACGATGAGGCCTTGTCCAACATGCCGATGCTGCTTGCCAGCATGGCGGAGGGACTTCTTCTCTCTCCTCCTTCTTTTTGCATGCATGACGACGTGGAGTGGGGTGACGTGGACATGAGTGATGACATGTCGCTGTGGAGCTTT

Coding sequence (CDS)

ATGGATATGTTCTTCGGTCAATTTTCCGATCCTCTTCCTTACGGCGACAAGTCGGAATCGTCGACGTGGTCGGACGCCGGCACGCCGGTTACTCACCCGGCGGCGCACTCCGATGAGGAAGTGATACTGGCGGCGAGCCGGCCGAAGCGGCGGGCCGGGCGGAGGGTTTTTAAGGAGACGAGGCACCCGGTTTACCGGGGGGTCCGGCGGAGGAACAACGATAAATGGGTGTGCGAGTTGCGTGAGCCGAATAAGAAGTCTAGGATCTGGCTCGGGACGTATCCGACGGCGGAGATGGCAGCGCGTGCGCACGACGTCGCGGCACTCGCGTTGAGAGGCAAGTCGGCTTGCCTCAACTTTGCTGACTCGGCGTGGCGATTTCCCATTCCGGAGTCTACTGATCCGAGTGTCATCCGGGACGCGGCGGCTCGGGCGGCCAATGAGTGCAAGGATGAAGAGGACGACGCCGTTTTGGGAGGCGGAGAAGGAGAACACCCCGCCAGGGGGGAGCCAGCTCCTCCGGCTTACGTGGACGATGAGGCCTTGTCCAACATGCCGATGCTGCTTGCCAGCATGGCGGAGGGACTTCTTCTCTCTCCTCCTTCTTTTTGCATGCATGACGACGTGGAGTGGGGTGACGTGGACATGAGTGATGACATGTCGCTGTGGAGCTTT

Protein sequence

MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLGGGEGEHPARGEPAPPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF
Homology
BLAST of MS018013 vs. NCBI nr
Match: XP_022159647.1 (dehydration-responsive element-binding protein 1E-like [Momordica charantia])

HSP 1 Score: 450.7 bits (1158), Expect = 7.6e-123
Identity = 222/225 (98.67%), Postives = 222/225 (98.67%), Query Frame = 0

Query: 1   MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKET 60
           MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKET
Sbjct: 1   MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKET 60

Query: 61  RHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNF 120
           RHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNF
Sbjct: 61  RHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNF 120

Query: 121 ADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLGGGEGEHPARGEPAPPAYVDDE 180
           ADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVL GGEGE PAR EPAPPAYVDDE
Sbjct: 121 ADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLEGGEGELPARWEPAPPAYVDDE 180

Query: 181 ALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
           ALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF
Sbjct: 181 ALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 225

BLAST of MS018013 vs. NCBI nr
Match: XP_038906461.1 (dehydration-responsive element-binding protein 1E-like [Benincasa hispida])

HSP 1 Score: 344.7 bits (883), Expect = 5.8e-91
Identity = 183/234 (78.21%), Postives = 195/234 (83.33%), Query Frame = 0

Query: 4   FFGQFSDPLPY----GDKSE-SSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFK 63
           +F QFSDPLPY      KSE SST SDAGTP    AA+SDEEVILA++RPKRRAGRRVFK
Sbjct: 3   WFAQFSDPLPYYAHCNYKSESSSTLSDAGTPPQTVAANSDEEVILASNRPKRRAGRRVFK 62

Query: 64  ETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACL 123
           ETRHPVYRGVRRRNNDKWVCELREPNKK+RIWLGTYPTAEMAARAHDVAALALRGKSACL
Sbjct: 63  ETRHPVYRGVRRRNNDKWVCELREPNKKTRIWLGTYPTAEMAARAHDVAALALRGKSACL 122

Query: 124 NFADSAWRFPIPESTDPSVIRDAAARAANECKD-------EEDDAVLGGGEGEHPARGEP 183
           NFADSAWRFP+P S DP+VIR+AAARAANECKD       EEDD VLG       A  EP
Sbjct: 123 NFADSAWRFPLPASDDPAVIREAAARAANECKDEEYNVGREEDDGVLG------QATAEP 182

Query: 184 APPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
             P Y+DDEA+SNMPMLLA MAEGLLLSPPSFC+ DDVEW DV MSDD+SLWSF
Sbjct: 183 NQPDYIDDEAMSNMPMLLAGMAEGLLLSPPSFCVTDDVEWDDVAMSDDVSLWSF 230

BLAST of MS018013 vs. NCBI nr
Match: XP_022958461.1 (dehydration-responsive element-binding protein 1E-like isoform X1 [Cucurbita moschata] >XP_022958462.1 dehydration-responsive element-binding protein 1E-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 322.0 bits (824), Expect = 4.1e-84
Identity = 174/234 (74.36%), Postives = 188/234 (80.34%), Query Frame = 0

Query: 4   FFGQFSDPLP----YGDKSE-SSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFK 63
           FF QFSDP+P    Y  KSE SST SDAGTP  H  A+SDEEVILA++RPKRRAGRRVFK
Sbjct: 3   FFTQFSDPIPYYGNYNCKSESSSTLSDAGTP-PHVVANSDEEVILASNRPKRRAGRRVFK 62

Query: 64  ETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACL 123
           ETRHPVYRGVRRRN  KWVCELREPNKK+RIWLGTYPTAEMAARAHDVAALALRGKSACL
Sbjct: 63  ETRHPVYRGVRRRNKGKWVCELREPNKKTRIWLGTYPTAEMAARAHDVAALALRGKSACL 122

Query: 124 NFADSAWRFPIPESTDPSVIRDAAARAANECK-------DEEDDAVLGGGEGEHPARGEP 183
           NFADSAWRFPIP S DPSVIR+AAA+AA EC+        EED+ VL         + EP
Sbjct: 123 NFADSAWRFPIPSSNDPSVIREAAAKAAKECEYEECNVSREEDEVVLS------QPKAEP 182

Query: 184 APPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
               YVDDEALSNMPMLLASMAEG LLSPP FC+ DDVEW +VD +DD+SLWSF
Sbjct: 183 DQRDYVDDEALSNMPMLLASMAEGPLLSPPRFCLRDDVEWDNVDTNDDVSLWSF 229

BLAST of MS018013 vs. NCBI nr
Match: XP_004143933.1 (dehydration-responsive element-binding protein 1E [Cucumis sativus])

HSP 1 Score: 322.0 bits (824), Expect = 4.1e-84
Identity = 170/229 (74.24%), Postives = 189/229 (82.53%), Query Frame = 0

Query: 4   FFGQFSDPLPY----GDKSE-SSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFK 63
           +FGQFSDPLPY      KSE SST SDAGTP      +SDEEVILA++RPKRRAGRR+FK
Sbjct: 3   WFGQFSDPLPYYPNCSYKSESSSTLSDAGTPPPQTLVNSDEEVILASNRPKRRAGRRIFK 62

Query: 64  ETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACL 123
           ETRHPVYRGVR+RNNDKWVCELREPNKK+RIWLGTYPTAEMAARAHD+AALALRGKSACL
Sbjct: 63  ETRHPVYRGVRQRNNDKWVCELREPNKKTRIWLGTYPTAEMAARAHDIAALALRGKSACL 122

Query: 124 NFADSAWRFPIPESTDPSVIRDAAARAANECK--DEEDDAVLGGGEGEHPARGEPAPPAY 183
           NFADSAWRFPIP S DP+VIR+AAARAANEC    EED+       G +     P    Y
Sbjct: 123 NFADSAWRFPIPTSDDPTVIREAAARAANECNVGREEDEG------GLNQPDTYPDGTDY 182

Query: 184 VDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
           VDDEA+SNMPMLLA+MAEGLLLSPPSFC++DDVEW D  M+DD+SLW+F
Sbjct: 183 VDDEAMSNMPMLLANMAEGLLLSPPSFCVNDDVEWDDEAMNDDVSLWNF 225

BLAST of MS018013 vs. NCBI nr
Match: XP_022922370.1 (dehydration-responsive element-binding protein 1A-like [Cucurbita moschata])

HSP 1 Score: 319.7 bits (818), Expect = 2.0e-83
Identity = 175/238 (73.53%), Postives = 189/238 (79.41%), Query Frame = 0

Query: 1   MDMFFGQFSDPLPY-----GDKSESSTWSDAGT-PVTHPAAHSDEEVILAASRPKRRAGR 60
           MD+ F QFSDPLPY      +   SS + DAGT P+T   A SDEEVILA+++PKR AGR
Sbjct: 1   MDL-FSQFSDPLPYYGNCNYNSESSSAFFDAGTHPLT--VATSDEEVILASNQPKRPAGR 60

Query: 61  RVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGK 120
           RVFKETRHPVYRGVRRRNNDKWVCE+REPNKK+RIWLGTYPTAEMAARAHDVAALALRGK
Sbjct: 61  RVFKETRHPVYRGVRRRNNDKWVCEMREPNKKTRIWLGTYPTAEMAARAHDVAALALRGK 120

Query: 121 SACLNFADSAWRFPIPESTDPSVIRDAAARAANECKD-------EEDDAVLGGGEGEHPA 180
           SACLNFADSAWRFPIP S DPSVIR AAA AANECKD       EEDD VL         
Sbjct: 121 SACLNFADSAWRFPIPASDDPSVIRAAAAMAANECKDEECNACREEDDVVL------CQP 180

Query: 181 RGEPAPPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
              P  P YVDDEALSNMPMLLA+MAEGLLLSPPSFC++DDVEW DV MS D+SLW+F
Sbjct: 181 TASPDQPVYVDDEALSNMPMLLANMAEGLLLSPPSFCVNDDVEWDDVAMSHDVSLWNF 229

BLAST of MS018013 vs. ExPASy Swiss-Prot
Match: Q9FJ93 (Dehydration-responsive element-binding protein 1D OS=Arabidopsis thaliana OX=3702 GN=DREB1D PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 2.1e-51
Identity = 121/216 (56.02%), Postives = 145/216 (67.13%), Query Frame = 0

Query: 24  SDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREP 83
           SD  +PV+  +  S +   LA+S PK+RAGR+ F+ETRHP+YRGVR+RN+ KWVCE+REP
Sbjct: 16  SDHRSPVSDSSECSPK---LASSCPKKRAGRKKFRETRHPIYRGVRQRNSGKWVCEVREP 75

Query: 84  NKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAA 143
           NKKSRIWLGT+PT EMAARAHDVAALALRG+SACLNFADSAWR  IPE+T P  I+ AA+
Sbjct: 76  NKKSRIWLGTFPTVEMAARAHDVAALALRGRSACLNFADSAWRLRIPETTCPKEIQKAAS 135

Query: 144 RAA----NECKDE--------EDDAVLGGGEGEHPARGEPAPPAYVDDEALSNMPMLLAS 203
            AA    NE   E        E+ A  G  EGE  A  +     Y+DDEAL  MP    +
Sbjct: 136 EAAMAFQNETTTEGSKTAAEAEEAAGEGVREGERRAEEQNGGVFYMDDEALLGMPNFFEN 195

Query: 204 MAEGLLLSPPSFCMHDDVEW--GDVDMSDDMSLWSF 226
           MAEG+LL PP      +V W   D D   D+SLWSF
Sbjct: 196 MAEGMLLPPP------EVGWNHNDFDGVGDVSLWSF 222

BLAST of MS018013 vs. ExPASy Swiss-Prot
Match: Q9LN86 (Dehydration-responsive element-binding protein 1F OS=Arabidopsis thaliana OX=3702 GN=DREB1F PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-49
Identity = 113/209 (54.07%), Postives = 134/209 (64.11%), Query Frame = 0

Query: 37  SDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPT 96
           +++++ILA  RPK+RAGRRVFKETRHPVYRG+RRRN DKWVCE+REP  + RIWLGTYPT
Sbjct: 2   NNDDIILAEMRPKKRAGRRVFKETRHPVYRGIRRRNGDKWVCEVREPTHQRRIWLGTYPT 61

Query: 97  AEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAAN--------- 156
           A+MAARAHDVA LALRG+SACLNFADSAWR P+PES DP VIR  AA AA          
Sbjct: 62  ADMAARAHDVAVLALRGRSACLNFADSAWRLPVPESNDPDVIRRVAAEAAEMFRPVDLES 121

Query: 157 -----ECKDEEDDAVLGGGEGEHPARGEPAPPAY--VDDEALSNMPMLLASMAEGLLLSP 216
                 C  ++ D   G G G      E    +Y   D E +S   M L   AEG L+SP
Sbjct: 122 GITVLPCAGDDVDLGFGSGSGSGSGSEERNSSSYGFGDYEEVSTTMMRL---AEGPLMSP 181

Query: 217 PSFCMHD----DVEWGDVDMSDDMSLWSF 226
           P   M D    +V   +    +DMSLWS+
Sbjct: 182 PRSYMEDMTPTNVYTEEEMCYEDMSLWSY 207

BLAST of MS018013 vs. ExPASy Swiss-Prot
Match: Q9M0L0 (Dehydration-responsive element-binding protein 1A OS=Arabidopsis thaliana OX=3702 GN=DREB1A PE=1 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 1.7e-48
Identity = 114/194 (58.76%), Postives = 134/194 (69.07%), Query Frame = 0

Query: 43  LAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAAR 102
           LA+S PK+ AGR+ F+ETRHP+YRGVRRRN+ KWVCE+REPNKK+RIWLGT+ TAEMAAR
Sbjct: 29  LASSCPKKPAGRKKFRETRHPIYRGVRRRNSGKWVCEVREPNKKTRIWLGTFQTAEMAAR 88

Query: 103 AHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLGGGE 162
           AHDVAALALRG+SACLNFADSAWR  IPEST    I+ AAA AA   +DE  DA    G 
Sbjct: 89  AHDVAALALRGRSACLNFADSAWRLRIPESTCAKDIQKAAAEAALAFQDEMCDATTDHGF 148

Query: 163 GEHPARGEPAPPA-------YVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWG--- 222
                  E    A       Y+ DEA+  MP LLA+MAEG+LL  PS      V+W    
Sbjct: 149 DMEETLVEAIYTAEQSENAFYMHDEAMFEMPSLLANMAEGMLLPLPS------VQWNHNH 208

Query: 223 DVD-MSDDMSLWSF 226
           +VD   DD+SLWS+
Sbjct: 209 EVDGDDDDVSLWSY 216

BLAST of MS018013 vs. ExPASy Swiss-Prot
Match: Q9SYS6 (Dehydration-responsive element-binding protein 1C OS=Arabidopsis thaliana OX=3702 GN=DREB1C PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 9.2e-47
Identity = 117/217 (53.92%), Postives = 145/217 (66.82%), Query Frame = 0

Query: 24  SDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREP 83
           SD  +PV+    +S +   LA S PK+ AGR+ F+ETRHP+YRGVR+RN+ KWVCELREP
Sbjct: 13  SDYESPVSSGGDYSPK---LATSCPKKPAGRKKFRETRHPIYRGVRQRNSGKWVCELREP 72

Query: 84  NKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAA 143
           NKK+RIWLGT+ TAEMAARAHDVAA+ALRG+SACLNFADSAWR  IPEST    I+ AAA
Sbjct: 73  NKKTRIWLGTFQTAEMAARAHDVAAIALRGRSACLNFADSAWRLRIPESTCAKEIQKAAA 132

Query: 144 RAANECKDE----EDDAVLGGGEGEH--------PARGEPAPPAYVDDEALSNMPMLLAS 203
            AA   +DE      DA   G + E         P + + A   Y+D+EA+  M  LL +
Sbjct: 133 EAALNFQDEMCHMTTDA--HGLDMEETLVEAIYTPEQSQDA--FYMDEEAMLGMSSLLDN 192

Query: 204 MAEGLLLSPPSFCMHDDVEWG---DVDMSDDMSLWSF 226
           MAEG+LL  PS      V+W    DV+  DD+SLWS+
Sbjct: 193 MAEGMLLPSPS------VQWNYNFDVEGDDDVSLWSY 216

BLAST of MS018013 vs. ExPASy Swiss-Prot
Match: P93835 (Dehydration-responsive element-binding protein 1B OS=Arabidopsis thaliana OX=3702 GN=DREB1B PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 4.6e-46
Identity = 110/196 (56.12%), Postives = 133/196 (67.86%), Query Frame = 0

Query: 43  LAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAAR 102
           LA S PK+ AGR+ F+ETRHP+YRGVR+RN+ KWV E+REPNKK+RIWLGT+ TAEMAAR
Sbjct: 26  LATSCPKKPAGRKKFRETRHPIYRGVRQRNSGKWVSEVREPNKKTRIWLGTFQTAEMAAR 85

Query: 103 AHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVL--GG 162
           AHDVAALALRG+SACLNFADSAWR  IPEST    I+ AAA AA   +DE  D      G
Sbjct: 86  AHDVAALALRGRSACLNFADSAWRLRIPESTCAKDIQKAAAEAALAFQDETCDTTTTNHG 145

Query: 163 GEGEH--------PARGEPAPPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWG 222
            + E         P + E A   Y+D+E +  MP LL +MAEG+LL PPS      V+W 
Sbjct: 146 LDMEETMVEAIYTPEQSEGA--FYMDEETMFGMPTLLDNMAEGMLLPPPS------VQWN 205

Query: 223 ---DVDMSDDMSLWSF 226
              D +   D+SLWS+
Sbjct: 206 HNYDGEGDGDVSLWSY 213

BLAST of MS018013 vs. ExPASy TrEMBL
Match: A0A6J1E0E0 (dehydration-responsive element-binding protein 1E-like OS=Momordica charantia OX=3673 GN=LOC111025993 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 3.7e-123
Identity = 222/225 (98.67%), Postives = 222/225 (98.67%), Query Frame = 0

Query: 1   MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKET 60
           MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKET
Sbjct: 1   MDMFFGQFSDPLPYGDKSESSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKET 60

Query: 61  RHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNF 120
           RHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNF
Sbjct: 61  RHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNF 120

Query: 121 ADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLGGGEGEHPARGEPAPPAYVDDE 180
           ADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVL GGEGE PAR EPAPPAYVDDE
Sbjct: 121 ADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLEGGEGELPARWEPAPPAYVDDE 180

Query: 181 ALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
           ALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF
Sbjct: 181 ALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 225

BLAST of MS018013 vs. ExPASy TrEMBL
Match: A0A6J1H1W9 (dehydration-responsive element-binding protein 1E-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459681 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.0e-84
Identity = 174/234 (74.36%), Postives = 188/234 (80.34%), Query Frame = 0

Query: 4   FFGQFSDPLP----YGDKSE-SSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFK 63
           FF QFSDP+P    Y  KSE SST SDAGTP  H  A+SDEEVILA++RPKRRAGRRVFK
Sbjct: 3   FFTQFSDPIPYYGNYNCKSESSSTLSDAGTP-PHVVANSDEEVILASNRPKRRAGRRVFK 62

Query: 64  ETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACL 123
           ETRHPVYRGVRRRN  KWVCELREPNKK+RIWLGTYPTAEMAARAHDVAALALRGKSACL
Sbjct: 63  ETRHPVYRGVRRRNKGKWVCELREPNKKTRIWLGTYPTAEMAARAHDVAALALRGKSACL 122

Query: 124 NFADSAWRFPIPESTDPSVIRDAAARAANECK-------DEEDDAVLGGGEGEHPARGEP 183
           NFADSAWRFPIP S DPSVIR+AAA+AA EC+        EED+ VL         + EP
Sbjct: 123 NFADSAWRFPIPSSNDPSVIREAAAKAAKECEYEECNVSREEDEVVLS------QPKAEP 182

Query: 184 APPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
               YVDDEALSNMPMLLASMAEG LLSPP FC+ DDVEW +VD +DD+SLWSF
Sbjct: 183 DQRDYVDDEALSNMPMLLASMAEGPLLSPPRFCLRDDVEWDNVDTNDDVSLWSF 229

BLAST of MS018013 vs. ExPASy TrEMBL
Match: A0A0A0KQZ2 (Dehydration-responsive element-binding protein 1F OS=Cucumis sativus OX=3659 GN=Csa_5G155570 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 2.0e-84
Identity = 170/229 (74.24%), Postives = 189/229 (82.53%), Query Frame = 0

Query: 4   FFGQFSDPLPY----GDKSE-SSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFK 63
           +FGQFSDPLPY      KSE SST SDAGTP      +SDEEVILA++RPKRRAGRR+FK
Sbjct: 3   WFGQFSDPLPYYPNCSYKSESSSTLSDAGTPPPQTLVNSDEEVILASNRPKRRAGRRIFK 62

Query: 64  ETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACL 123
           ETRHPVYRGVR+RNNDKWVCELREPNKK+RIWLGTYPTAEMAARAHD+AALALRGKSACL
Sbjct: 63  ETRHPVYRGVRQRNNDKWVCELREPNKKTRIWLGTYPTAEMAARAHDIAALALRGKSACL 122

Query: 124 NFADSAWRFPIPESTDPSVIRDAAARAANECK--DEEDDAVLGGGEGEHPARGEPAPPAY 183
           NFADSAWRFPIP S DP+VIR+AAARAANEC    EED+       G +     P    Y
Sbjct: 123 NFADSAWRFPIPTSDDPTVIREAAARAANECNVGREEDEG------GLNQPDTYPDGTDY 182

Query: 184 VDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
           VDDEA+SNMPMLLA+MAEGLLLSPPSFC++DDVEW D  M+DD+SLW+F
Sbjct: 183 VDDEAMSNMPMLLANMAEGLLLSPPSFCVNDDVEWDDEAMNDDVSLWNF 225

BLAST of MS018013 vs. ExPASy TrEMBL
Match: A0A6J1E8J8 (dehydration-responsive element-binding protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111430380 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 9.8e-84
Identity = 175/238 (73.53%), Postives = 189/238 (79.41%), Query Frame = 0

Query: 1   MDMFFGQFSDPLPY-----GDKSESSTWSDAGT-PVTHPAAHSDEEVILAASRPKRRAGR 60
           MD+ F QFSDPLPY      +   SS + DAGT P+T   A SDEEVILA+++PKR AGR
Sbjct: 1   MDL-FSQFSDPLPYYGNCNYNSESSSAFFDAGTHPLT--VATSDEEVILASNQPKRPAGR 60

Query: 61  RVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGK 120
           RVFKETRHPVYRGVRRRNNDKWVCE+REPNKK+RIWLGTYPTAEMAARAHDVAALALRGK
Sbjct: 61  RVFKETRHPVYRGVRRRNNDKWVCEMREPNKKTRIWLGTYPTAEMAARAHDVAALALRGK 120

Query: 121 SACLNFADSAWRFPIPESTDPSVIRDAAARAANECKD-------EEDDAVLGGGEGEHPA 180
           SACLNFADSAWRFPIP S DPSVIR AAA AANECKD       EEDD VL         
Sbjct: 121 SACLNFADSAWRFPIPASDDPSVIRAAAAMAANECKDEECNACREEDDVVL------CQP 180

Query: 181 RGEPAPPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
              P  P YVDDEALSNMPMLLA+MAEGLLLSPPSFC++DDVEW DV MS D+SLW+F
Sbjct: 181 TASPDQPVYVDDEALSNMPMLLANMAEGLLLSPPSFCVNDDVEWDDVAMSHDVSLWNF 229

BLAST of MS018013 vs. ExPASy TrEMBL
Match: A0A6J1K939 (dehydration-responsive element-binding protein 1C-like OS=Cucurbita maxima OX=3661 GN=LOC111491237 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.8e-83
Identity = 173/234 (73.93%), Postives = 187/234 (79.91%), Query Frame = 0

Query: 4   FFGQFSDPLP----YGDKSE-SSTWSDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFK 63
           FF QFSDP+P    Y  KSE SST SDAGTP+ H  A+SDEEVILA++RPKRRAGRRVFK
Sbjct: 3   FFTQFSDPIPYYGNYNYKSESSSTLSDAGTPL-HVVANSDEEVILASNRPKRRAGRRVFK 62

Query: 64  ETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAARAHDVAALALRGKSACL 123
           ETRHPVYRGVRRRN  KWVCELREPNKK+RIWLGTY TAEMAARAHDVAALALRGKSACL
Sbjct: 63  ETRHPVYRGVRRRNKGKWVCELREPNKKTRIWLGTYLTAEMAARAHDVAALALRGKSACL 122

Query: 124 NFADSAWRFPIPESTDPSVIRDAAARAANECK-------DEEDDAVLGGGEGEHPARGEP 183
           NFADSAWRFPIP S DPSVIR+AAA+AA EC+        EED+ VL         + EP
Sbjct: 123 NFADSAWRFPIPSSNDPSVIREAAAKAAKECEYEECNVSREEDEVVLS------QPKAEP 182

Query: 184 APPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWGDVDMSDDMSLWSF 226
               YVDDEAL NMPMLLASMAEG LLSPP FC+ DDVEW DVD +DD+SLWSF
Sbjct: 183 DQRDYVDDEALLNMPMLLASMAEGPLLSPPHFCVRDDVEWDDVDTNDDVSLWSF 229

BLAST of MS018013 vs. TAIR 10
Match: AT5G51990.1 (C-repeat-binding factor 4 )

HSP 1 Score: 203.8 bits (517), Expect = 1.5e-52
Identity = 121/216 (56.02%), Postives = 145/216 (67.13%), Query Frame = 0

Query: 24  SDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREP 83
           SD  +PV+  +  S +   LA+S PK+RAGR+ F+ETRHP+YRGVR+RN+ KWVCE+REP
Sbjct: 16  SDHRSPVSDSSECSPK---LASSCPKKRAGRKKFRETRHPIYRGVRQRNSGKWVCEVREP 75

Query: 84  NKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAA 143
           NKKSRIWLGT+PT EMAARAHDVAALALRG+SACLNFADSAWR  IPE+T P  I+ AA+
Sbjct: 76  NKKSRIWLGTFPTVEMAARAHDVAALALRGRSACLNFADSAWRLRIPETTCPKEIQKAAS 135

Query: 144 RAA----NECKDE--------EDDAVLGGGEGEHPARGEPAPPAYVDDEALSNMPMLLAS 203
            AA    NE   E        E+ A  G  EGE  A  +     Y+DDEAL  MP    +
Sbjct: 136 EAAMAFQNETTTEGSKTAAEAEEAAGEGVREGERRAEEQNGGVFYMDDEALLGMPNFFEN 195

Query: 204 MAEGLLLSPPSFCMHDDVEW--GDVDMSDDMSLWSF 226
           MAEG+LL PP      +V W   D D   D+SLWSF
Sbjct: 196 MAEGMLLPPP------EVGWNHNDFDGVGDVSLWSF 222

BLAST of MS018013 vs. TAIR 10
Match: AT1G12610.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.1e-50
Identity = 113/209 (54.07%), Postives = 134/209 (64.11%), Query Frame = 0

Query: 37  SDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPT 96
           +++++ILA  RPK+RAGRRVFKETRHPVYRG+RRRN DKWVCE+REP  + RIWLGTYPT
Sbjct: 2   NNDDIILAEMRPKKRAGRRVFKETRHPVYRGIRRRNGDKWVCEVREPTHQRRIWLGTYPT 61

Query: 97  AEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAAN--------- 156
           A+MAARAHDVA LALRG+SACLNFADSAWR P+PES DP VIR  AA AA          
Sbjct: 62  ADMAARAHDVAVLALRGRSACLNFADSAWRLPVPESNDPDVIRRVAAEAAEMFRPVDLES 121

Query: 157 -----ECKDEEDDAVLGGGEGEHPARGEPAPPAY--VDDEALSNMPMLLASMAEGLLLSP 216
                 C  ++ D   G G G      E    +Y   D E +S   M L   AEG L+SP
Sbjct: 122 GITVLPCAGDDVDLGFGSGSGSGSGSEERNSSSYGFGDYEEVSTTMMRL---AEGPLMSP 181

Query: 217 PSFCMHD----DVEWGDVDMSDDMSLWSF 226
           P   M D    +V   +    +DMSLWS+
Sbjct: 182 PRSYMEDMTPTNVYTEEEMCYEDMSLWSY 207

BLAST of MS018013 vs. TAIR 10
Match: AT4G25480.1 (dehydration response element B1A )

HSP 1 Score: 194.1 bits (492), Expect = 1.2e-49
Identity = 114/194 (58.76%), Postives = 134/194 (69.07%), Query Frame = 0

Query: 43  LAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAAR 102
           LA+S PK+ AGR+ F+ETRHP+YRGVRRRN+ KWVCE+REPNKK+RIWLGT+ TAEMAAR
Sbjct: 29  LASSCPKKPAGRKKFRETRHPIYRGVRRRNSGKWVCEVREPNKKTRIWLGTFQTAEMAAR 88

Query: 103 AHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVLGGGE 162
           AHDVAALALRG+SACLNFADSAWR  IPEST    I+ AAA AA   +DE  DA    G 
Sbjct: 89  AHDVAALALRGRSACLNFADSAWRLRIPESTCAKDIQKAAAEAALAFQDEMCDATTDHGF 148

Query: 163 GEHPARGEPAPPA-------YVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWG--- 222
                  E    A       Y+ DEA+  MP LLA+MAEG+LL  PS      V+W    
Sbjct: 149 DMEETLVEAIYTAEQSENAFYMHDEAMFEMPSLLANMAEGMLLPLPS------VQWNHNH 208

Query: 223 DVD-MSDDMSLWSF 226
           +VD   DD+SLWS+
Sbjct: 209 EVDGDDDDVSLWSY 216

BLAST of MS018013 vs. TAIR 10
Match: AT4G25470.1 (C-repeat/DRE binding factor 2 )

HSP 1 Score: 188.3 bits (477), Expect = 6.5e-48
Identity = 117/217 (53.92%), Postives = 145/217 (66.82%), Query Frame = 0

Query: 24  SDAGTPVTHPAAHSDEEVILAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREP 83
           SD  +PV+    +S +   LA S PK+ AGR+ F+ETRHP+YRGVR+RN+ KWVCELREP
Sbjct: 13  SDYESPVSSGGDYSPK---LATSCPKKPAGRKKFRETRHPIYRGVRQRNSGKWVCELREP 72

Query: 84  NKKSRIWLGTYPTAEMAARAHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAA 143
           NKK+RIWLGT+ TAEMAARAHDVAA+ALRG+SACLNFADSAWR  IPEST    I+ AAA
Sbjct: 73  NKKTRIWLGTFQTAEMAARAHDVAAIALRGRSACLNFADSAWRLRIPESTCAKEIQKAAA 132

Query: 144 RAANECKDE----EDDAVLGGGEGEH--------PARGEPAPPAYVDDEALSNMPMLLAS 203
            AA   +DE      DA   G + E         P + + A   Y+D+EA+  M  LL +
Sbjct: 133 EAALNFQDEMCHMTTDA--HGLDMEETLVEAIYTPEQSQDA--FYMDEEAMLGMSSLLDN 192

Query: 204 MAEGLLLSPPSFCMHDDVEWG---DVDMSDDMSLWSF 226
           MAEG+LL  PS      V+W    DV+  DD+SLWS+
Sbjct: 193 MAEGMLLPSPS------VQWNYNFDVEGDDDVSLWSY 216

BLAST of MS018013 vs. TAIR 10
Match: AT4G25490.1 (C-repeat/DRE binding factor 1 )

HSP 1 Score: 186.0 bits (471), Expect = 3.2e-47
Identity = 110/196 (56.12%), Postives = 133/196 (67.86%), Query Frame = 0

Query: 43  LAASRPKRRAGRRVFKETRHPVYRGVRRRNNDKWVCELREPNKKSRIWLGTYPTAEMAAR 102
           LA S PK+ AGR+ F+ETRHP+YRGVR+RN+ KWV E+REPNKK+RIWLGT+ TAEMAAR
Sbjct: 26  LATSCPKKPAGRKKFRETRHPIYRGVRQRNSGKWVSEVREPNKKTRIWLGTFQTAEMAAR 85

Query: 103 AHDVAALALRGKSACLNFADSAWRFPIPESTDPSVIRDAAARAANECKDEEDDAVL--GG 162
           AHDVAALALRG+SACLNFADSAWR  IPEST    I+ AAA AA   +DE  D      G
Sbjct: 86  AHDVAALALRGRSACLNFADSAWRLRIPESTCAKDIQKAAAEAALAFQDETCDTTTTNHG 145

Query: 163 GEGEH--------PARGEPAPPAYVDDEALSNMPMLLASMAEGLLLSPPSFCMHDDVEWG 222
            + E         P + E A   Y+D+E +  MP LL +MAEG+LL PPS      V+W 
Sbjct: 146 LDMEETMVEAIYTPEQSEGA--FYMDEETMFGMPTLLDNMAEGMLLPPPS------VQWN 205

Query: 223 ---DVDMSDDMSLWSF 226
              D +   D+SLWS+
Sbjct: 206 HNYDGEGDGDVSLWSY 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159647.17.6e-12398.67dehydration-responsive element-binding protein 1E-like [Momordica charantia][more]
XP_038906461.15.8e-9178.21dehydration-responsive element-binding protein 1E-like [Benincasa hispida][more]
XP_022958461.14.1e-8474.36dehydration-responsive element-binding protein 1E-like isoform X1 [Cucurbita mos... [more]
XP_004143933.14.1e-8474.24dehydration-responsive element-binding protein 1E [Cucumis sativus][more]
XP_022922370.12.0e-8373.53dehydration-responsive element-binding protein 1A-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FJ932.1e-5156.02Dehydration-responsive element-binding protein 1D OS=Arabidopsis thaliana OX=370... [more]
Q9LN861.5e-4954.07Dehydration-responsive element-binding protein 1F OS=Arabidopsis thaliana OX=370... [more]
Q9M0L01.7e-4858.76Dehydration-responsive element-binding protein 1A OS=Arabidopsis thaliana OX=370... [more]
Q9SYS69.2e-4753.92Dehydration-responsive element-binding protein 1C OS=Arabidopsis thaliana OX=370... [more]
P938354.6e-4656.12Dehydration-responsive element-binding protein 1B OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A6J1E0E03.7e-12398.67dehydration-responsive element-binding protein 1E-like OS=Momordica charantia OX... [more]
A0A6J1H1W92.0e-8474.36dehydration-responsive element-binding protein 1E-like isoform X1 OS=Cucurbita m... [more]
A0A0A0KQZ22.0e-8474.24Dehydration-responsive element-binding protein 1F OS=Cucumis sativus OX=3659 GN=... [more]
A0A6J1E8J89.8e-8473.53dehydration-responsive element-binding protein 1A-like OS=Cucurbita moschata OX=... [more]
A0A6J1K9392.8e-8373.93dehydration-responsive element-binding protein 1C-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT5G51990.11.5e-5256.02C-repeat-binding factor 4 [more]
AT1G12610.11.1e-5054.07Integrase-type DNA-binding superfamily protein [more]
AT4G25480.11.2e-4958.76dehydration response element B1A [more]
AT4G25470.16.5e-4853.92C-repeat/DRE binding factor 2 [more]
AT4G25490.13.2e-4756.12C-repeat/DRE binding factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 65..76
score: 57.88
coord: 87..103
score: 66.1
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 64..127
e-value: 1.1E-27
score: 107.9
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 63..113
e-value: 2.1E-14
score: 53.5
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 64..121
score: 22.009764
IPR001471AP2/ERF domainCDDcd00018AP2coord: 65..123
e-value: 2.21737E-29
score: 102.735
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 64..122
e-value: 3.8E-23
score: 83.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..174
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR31839:SF64DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1Fcoord: 4..225
NoneNo IPR availablePANTHERPTHR31839DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1Dcoord: 4..225
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 64..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS018013.1MS018013.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity