MS018003 (gene) Bitter gourd (TR) v1

Overview


Name	MS018003
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RHOMBOID-like protein
Location	scaffold373: 4576565 .. 4578485 (+)
RNA-Seq Expression	MS018003
Synteny	MS018003

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTAATCAAGATCTGGAGCGAGGAGCGGCTAAGAATCAAGCCAACACTAACCCAAATCGGAACTATTCAGCTTCTCCTTACTACATAGAGACTTCTGAGAAGCAATGGACGCCATGGCTGATTCCTATGTTCGTCGTCGCCAATGTCGCTATGTTTATTGTGGTCATGTACGTCAATAATTGCCCTACCAACAACAATCGGGGTTCTCGAGATAAGTGTGTCGCCAATTTCCTCGGCAGATTTTCGTTTCAGCCTCTCAACGAGAATCCCCTCTTCGGACCCTCTTCTTCTACGTATGTCTTCTTTCTGCTCTCTTCCTTTTCATGGAGTTTTAATTCCCATGAGTTTGATTCTCTGCATACTTCTGAATTCGGTTTGCTCCTAAGGAATTCTGTGGATTGGATCCTATTTGGGTTTGATTGAACCCATTGGCTTAAACAACCTTATTCCTCTGCAGGTTGGAGAAATTGGGAGCTCTGAAGTGGGACAGGGTTGTTCATCAGCATCAAGGATGGCGACTTCTCTCATGTATCTGGCTGCACGCTGGAATCATTCATCTTCTTGCAAATATGTTGAGTTTGGTTCTGATTGGGATCCGCCTTGAGCAGCAGTTTGGATTTGGTATGTCTCCTTTTTTTTCCCTTCTTGCGAGCTTCAATTTGTAAAGTTTTCAAAATTTGAACTCCAAATCATGTTAGATGTTGCACCATGTTCCGTACGTCTTAGTGAAAAGAGTAGTCGAATGTAATCAATATTTCATCTTTTGTTTTCCCTTTAGGAAGGTGGTGTTGAATTTTACAATTACCCCATGCGAGCTTTTTGTTAAAAAAAAAAAAAAAACATTTTCTGCTTGCTAAGTAATGGTGTGTAGTTTTTGAATTACCCATGATGAGCTTTTTTTATCAAATAAACATATGCACACATCCATTTACATATGCACGTGAACTTAAAAGACCTCCACTTTAAATTCCAACAGTACAGCAAGAGTGTTAGCTTCAAACTGTTTGCGAACAAAAGGTTTGGGGAAATATGGGACGAAATTTCTCTTCCCATGGCAGAAATTTTAACTACTCACATTTTATTTTCTCCCTTCATACTTTCCTTCCCTGTCTGAGCATCTTTTAATTTTTTTCAGTGCGAATTGGAATAATATATCTTGTGGCTGGAGTTGGTGGGAGTACGATGTCTTCCCTGTTCATCCGAAACAATATTTCTGTTGGAGCTTCTGGTGCCCTCTTTGGACTTCTCGGAGCTATGCTATCCGAGCTTTTGACGAACTGGACGATTTACACAAATAAGGTTTGCTGCCAGTCATTGACTTCAACTTCGGGTTTTTGCATTTGTGATAGTTGTCTCTGTGAAGCTTTACTGTGACGAATTTCCAAATCTAATAAACTAATTCATGTTTTTGTAGGCTGCTGCATTCTTCACCCTCATCGTTATCGTAGCAATTAACTTGGCAGTCGGAATTCTACCTCACGTAGACAACTTCGCCCACATCGGAGGATTCTTAGCAGGATTCCTTCTCGGGTTTGTGTTTCTTATCCGACCCCAGTTTAAATGGACAGAACGCCACCATCTCCCACCCAGTGCTCGCCGCGTATCTAAGTACAAAACTTACCAATACATTCTCTGGTTGGCTGCTGCAATTCTACTAGTTGCTGGGTAAACCTCAAACCCTTTAGCTTTTTCCATATAGTAGTAGTACACGTTTCTTTCAACTAAATAGCATCTGATAGTATCCAACTGGTCCTCGTTAACGACATATTTGAATTGAGTAAGCTAATTTATGTTGTTGATTCGTTGCAGCTTTACTGTGGGGCTGGTGATGCTGTTCAGAGGTGAGAATGGTAACGATCACTGCAGCTGGTGCCATTACCTAAGTTGTGTGCCAACCTCAAAATGGGACTGTGGGAAT

mRNA sequence

ATGTCTAATCAAGATCTGGAGCGAGGAGCGGCTAAGAATCAAGCCAACACTAACCCAAATCGGAACTATTCAGCTTCTCCTTACTACATAGAGACTTCTGAGAAGCAATGGACGCCATGGCTGATTCCTATGTTCGTCGTCGCCAATGTCGCTATGTTTATTGTGGTCATGTACGTCAATAATTGCCCTACCAACAACAATCGGGGTTCTCGAGATAAGTGTGTCGCCAATTTCCTCGGCAGATTTTCGTTTCAGCCTCTCAACGAGAATCCCCTCTTCGGACCCTCTTCTTCTACGTTGGAGAAATTGGGAGCTCTGAAGTGGGACAGGGTTGTTCATCAGCATCAAGGATGGCGACTTCTCTCATGTATCTGGCTGCACGCTGGAATCATTCATCTTCTTGCAAATATGTTGAGTTTGGTTCTGATTGGGATCCGCCTTGAGCAGCAGTTTGGATTTGTGCGAATTGGAATAATATATCTTGTGGCTGGAGTTGGTGGGAGTACGATGTCTTCCCTGTTCATCCGAAACAATATTTCTGTTGGAGCTTCTGGTGCCCTCTTTGGACTTCTCGGAGCTATGCTATCCGAGCTTTTGACGAACTGGACGATTTACACAAATAAGGCTGCTGCATTCTTCACCCTCATCGTTATCGTAGCAATTAACTTGGCAGTCGGAATTCTACCTCACGTAGACAACTTCGCCCACATCGGAGGATTCTTAGCAGGATTCCTTCTCGGGTTTGTGTTTCTTATCCGACCCCAGTTTAAATGGACAGAACGCCACCATCTCCCACCCAGTGCTCGCCGCGTATCTAAGTACAAAACTTACCAATACATTCTCTGGTTGGCTGCTGCAATTCTACTAGTTGCTGGCTTTACTGTGGGGCTGGTGATGCTGTTCAGAGGTGAGAATGGTAACGATCACTGCAGCTGGTGCCATTACCTAAGTTGTGTGCCAACCTCAAAATGGGACTGTGGGAAT

Coding sequence (CDS)

ATGTCTAATCAAGATCTGGAGCGAGGAGCGGCTAAGAATCAAGCCAACACTAACCCAAATCGGAACTATTCAGCTTCTCCTTACTACATAGAGACTTCTGAGAAGCAATGGACGCCATGGCTGATTCCTATGTTCGTCGTCGCCAATGTCGCTATGTTTATTGTGGTCATGTACGTCAATAATTGCCCTACCAACAACAATCGGGGTTCTCGAGATAAGTGTGTCGCCAATTTCCTCGGCAGATTTTCGTTTCAGCCTCTCAACGAGAATCCCCTCTTCGGACCCTCTTCTTCTACGTTGGAGAAATTGGGAGCTCTGAAGTGGGACAGGGTTGTTCATCAGCATCAAGGATGGCGACTTCTCTCATGTATCTGGCTGCACGCTGGAATCATTCATCTTCTTGCAAATATGTTGAGTTTGGTTCTGATTGGGATCCGCCTTGAGCAGCAGTTTGGATTTGTGCGAATTGGAATAATATATCTTGTGGCTGGAGTTGGTGGGAGTACGATGTCTTCCCTGTTCATCCGAAACAATATTTCTGTTGGAGCTTCTGGTGCCCTCTTTGGACTTCTCGGAGCTATGCTATCCGAGCTTTTGACGAACTGGACGATTTACACAAATAAGGCTGCTGCATTCTTCACCCTCATCGTTATCGTAGCAATTAACTTGGCAGTCGGAATTCTACCTCACGTAGACAACTTCGCCCACATCGGAGGATTCTTAGCAGGATTCCTTCTCGGGTTTGTGTTTCTTATCCGACCCCAGTTTAAATGGACAGAACGCCACCATCTCCCACCCAGTGCTCGCCGCGTATCTAAGTACAAAACTTACCAATACATTCTCTGGTTGGCTGCTGCAATTCTACTAGTTGCTGGCTTTACTGTGGGGCTGGTGATGCTGTTCAGAGGTGAGAATGGTAACGATCACTGCAGCTGGTGCCATTACCTAAGTTGTGTGCCAACCTCAAAATGGGACTGTGGGAAT

Protein sequence

MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDCGN

Homology

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BLAST of MS018003 vs. NCBI nr
Match: XP_022158027.1 (RHOMBOID-like protein 2 [Momordica charantia])

HSP 1 Score: 668.3 bits (1723), Expect = 3.4e-188
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL
Sbjct: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML
Sbjct: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGNDHCSWCHYLSCVPTSKWDCGN
Sbjct: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 328

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BLAST of MS018003 vs. NCBI nr
Match: XP_038874691.1 (RHOMBOID-like protein 2 [Benincasa hispida])

HSP 1 Score: 602.8 bits (1553), Expect = 1.7e-168
Identity = 294/328 (89.63%), Postives = 307/328 (93.60%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  NTNPN NYS SPYYIETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNGNYSTSPYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           +CP N+N G RDKCVA FLGRFSFQPL ENPL GPSS+TL KLGALKWD+VVH+HQGWRL
Sbjct: 61  DCP-NSNLGFRDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
            SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 FSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAA FTLIVIV INLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV L+RPQFKWTERH+LPP A+RV KYKTYQYILWLAAAILLVAG TVG+VML
Sbjct: 241 LTGFLLGFVLLVRPQFKWTERHYLPPDAQRVPKYKTYQYILWLAAAILLVAGLTVGMVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDCGN
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCGN 327

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BLAST of MS018003 vs. NCBI nr
Match: XP_004143929.1 (RHOMBOID-like protein 2 [Cucumis sativus] >KGN50139.1 hypothetical protein Csa_000665 [Cucumis sativus])

HSP 1 Score: 599.7 bits (1545), Expect = 1.5e-167
Identity = 291/328 (88.72%), Postives = 307/328 (93.60%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  NTNPN NYS SPYY+ETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSTSPYYLETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP ++N G  DKCVA+FLGRFSFQP+ ENPL GPSS+TL KLGALKW++VVH+HQGWRL
Sbjct: 61  NCP-DSNLGFGDKCVASFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIG+IYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGMIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNK AA FTLIVIV INLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKVAALFTLIVIVVINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV L+RPQFKWTERHHLPP ARRV KYKTYQYILWLAAAILLVAGFT+GLVML
Sbjct: 241 LTGFLLGFVLLVRPQFKWTERHHLPPGARRVPKYKTYQYILWLAAAILLVAGFTLGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDC N
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCAN 327

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BLAST of MS018003 vs. NCBI nr
Match: KAG6606117.1 (RHOMBOID-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597.4 bits (1539), Expect = 7.3e-167
Identity = 292/328 (89.02%), Postives = 305/328 (92.99%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  N+NP  NYSA PY++ETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPTNSNPTGNYSAPPYHVETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP   N G  DKCVA FLGRFSFQPL ENPL GPSS+TL KLGALKWD+VVH+HQGWRL
Sbjct: 61  NCP-KTNLGVGDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIGIIYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAA FTLIVIVAINLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV LIRPQFKW ERHHLPPSA+RVSKYK YQYILW+ AAILLVAGFTVGLVML
Sbjct: 241 LTGFLLGFVLLIRPQFKWIERHHLPPSAQRVSKYKIYQYILWIVAAILLVAGFTVGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDCGN
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCGN 327

[Close]

BLAST of MS018003 vs. NCBI nr
Match: KAG7036061.1 (RHOMBOID-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.0 bits (1538), Expect = 9.5e-167
Identity = 292/328 (89.02%), Postives = 305/328 (92.99%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  N+NP  NYSA PY++ETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNSNPTGNYSAPPYHVETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP   N G  DKCVA FLGRFSFQPL ENPL GPSS+TL KLGALKWD+VVH+HQGWRL
Sbjct: 61  NCP-KTNLGVGDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIGIIYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAA FTLIVIVAINLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV LIRPQFKW ERHHLPPSA+RVSKYK YQYILW+ AAILLVAGFTVGLVML
Sbjct: 241 LTGFLLGFVLLIRPQFKWIERHHLPPSAQRVSKYKIYQYILWIVAAILLVAGFTVGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDCGN
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCGN 327

[Close]

BLAST of MS018003 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 3.2e-125
Identity = 209/292 (71.58%), Postives = 244/292 (83.56%), Query Frame = 0


Query: 36  QWTPWLIPMFVVANVAMFIVVMYVNNCPTN-NNRGSRDKCVANFLGRFSFQPLNENPLFG 95
           +WT WL+PMFVVANVA+F+V M+VNNCP +  +   R  CVA FLGR SF+PL  NPLFG
Sbjct: 52  RWTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFG 111

Query: 96  PSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 155
           PSS TLEKLGAL+W +VV + +GWRLL+CIWLHAG+IHL ANMLSLV IGIRLEQQFGFV
Sbjct: 112 PSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFV 171

Query: 156 RIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFT 215
           RIG+IYL++G+GGS +SSLFIRN+ISVGASGALFGLLG+MLSEL TNWTIY+NK AA  T
Sbjct: 172 RIGVIYLLSGIGGSVLSSLFIRNSISVGASGALFGLLGSMLSELFTNWTIYSNKIAALLT 231

Query: 216 LIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKY 275
           L+ ++ INLA+GILPHVDNFAH+GGF+ GFLLGF+ L RPQFKW  R H+P       KY
Sbjct: 232 LLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPLRYKY 291

Query: 276 KTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
           KTYQY+LWL + +LL+AGF V L+MLFRGENGNDHC WCHYL CVPTS W C
Sbjct: 292 KTYQYLLWLLSLVLLIAGFVVALLMLFRGENGNDHCRWCHYLRCVPTSSWRC 343

[Close]

BLAST of MS018003 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.0e-123
Identity = 219/327 (66.97%), Postives = 260/327 (79.51%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETS-EKQWTPWLIPMFVVANVAMFIVVMYV 60
           M+N+D+ER   KN+     N NY    +Y E+S E  WT WLIP  VVAN+A+FI VM+V
Sbjct: 1   MANRDVERVGKKNR---GANNNY----FYEESSGETHWTSWLIPAIVVANLAVFIAVMFV 60

Query: 61  NNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWR 120
           N+CP     G   +CVA FLGRFSFQPL ENPLFGPSSSTLEK+GAL+W +VVH+HQGWR
Sbjct: 61  NDCP-KKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWR 120

Query: 121 LLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNI 180
           LLSC+WLHAGIIHLL NMLSL+ IGIRLEQQFGF+R+G+IYL++G+GGS +SSLF++ +I
Sbjct: 121 LLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESI 180

Query: 181 SVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGG 240
           SVGASGALFGLLGAMLSELLTNWTIY NKAAA  TL+ I+AINLA+G+LP VDNFAHIGG
Sbjct: 181 SVGASGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGG 240

Query: 241 FLAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVM 300
           FL GF LGFV L+RPQ+ W        ++R   KY  YQY+L++ + +LLV G TV LVM
Sbjct: 241 FLTGFCLGFVLLVRPQYGWEASR--TNTSRTKRKYSMYQYVLFVVSVVLLVVGLTVALVM 300

Query: 301 LFRGENGNDHCSWCHYLSCVPTSKWDC 327
           LF+GENGN HC WCHYLSC PTSKW C
Sbjct: 301 LFKGENGNKHCKWCHYLSCFPTSKWTC 317

[Close]

BLAST of MS018003 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 6.3e-113
Identity = 196/297 (65.99%), Postives = 238/297 (80.13%), Query Frame = 0


Query: 35  KQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFG 94
           K W PWL+P  VVAN+A+F + M++NNCP N+       C+A FLGRF+FQP+ ENPL G
Sbjct: 52  KLWFPWLVPAIVVANIALFAISMFINNCPKNS-----AYCLARFLGRFAFQPMKENPLLG 111

Query: 95  PSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 154
           PSS TLEK+GAL    VVH+H+ WRL +CIWLHAG+ H+LANMLSL+ IGIRLEQ+FGFV
Sbjct: 112 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 171

Query: 155 RIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFT 214
           RIG++Y+++G GGS +SSLF R  ISVGASGALFGLLGAMLSELLTNWTIY NK AA  T
Sbjct: 172 RIGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLT 231

Query: 215 LIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTERHHLP-----PSAR 274
           LI I+AINLAVGILPHVDNFAH+GGF +GFLLGFVFLIRPQ+ +  + + P     PSA+
Sbjct: 232 LIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAK 291

Query: 275 RVSKYKTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
             SK+K YQY+LW+ + +LL+AG+T GLV+L RG + N HCSWCHYLSC+PTS W C
Sbjct: 292 --SKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSLWSC 341

[Close]

BLAST of MS018003 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.2e-105
Identity = 185/294 (62.93%), Postives = 224/294 (76.19%), Query Frame = 0


Query: 34  EKQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLF 93
           + QWT WL P  VVANV++FIVVMY N+CP     G+   CVA  L RFSFQPL ENP  
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCP-KTTTGANGDCVAKLLRRFSFQPLRENPFL 74

Query: 94  GPSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGF 153
           GPSSSTLEKLGAL W +VV  ++ WRL++ +WLHAGIIHL+ NM  +++ GIRLEQQFGF
Sbjct: 75  GPSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGF 134

Query: 154 VRIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFF 213
           +RIG+IYL++G GGS +S+LF++ +ISVGASGAL GL+GAMLSELLTNWTIY +K  A  
Sbjct: 135 IRIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALL 194

Query: 214 TLIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTE-RHHLPPSARRVS 273
           + + I+AINLA+G+LP VDNFAHIGG L GF LGF+ L++PQ  W E R+     AR  S
Sbjct: 195 SFLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARS 254

Query: 274 KYKTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
           KY   QY+L+  AA+L+VAG TVGLVMLF GENGN HC WCH L C PTSKW C
Sbjct: 255 KYNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

[Close]

BLAST of MS018003 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.3e-104
Identity = 177/292 (60.62%), Postives = 223/292 (76.37%), Query Frame = 0


Query: 35  KQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFG 94
           + W PWLIP FVVANVA+F++ MYVNNCP  +       C A+FLGRFSFQ   ENPL G
Sbjct: 62  RSWFPWLIPCFVVANVAVFVITMYVNNCPKKSG-----DCFADFLGRFSFQNTRENPLLG 121

Query: 95  PSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 154
           PSS TL+ +G L   +VV   +GWRLLSC WLH G++HLL NML+L+ IGIR+E++FGF+
Sbjct: 122 PSSLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFI 181

Query: 155 RIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFT 214
           RIG++YL++G GGS +S+LF+R+NISVGASGA+FGLLG MLSE+  NWTIY+NK     T
Sbjct: 182 RIGLLYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVT 241

Query: 215 LIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKY 274
           L++IVA+NL +G+LP VDNFAHIGGF  GFLLGFV LIRP + W  + + P    +  ++
Sbjct: 242 LVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGWINQRNGP--GAKPHRF 301

Query: 275 KTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
           K YQ ILW  + ++LVAGF VGL+ LF   +GN+HCSWCHYLSCVPTSKW C
Sbjct: 302 KIYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSWCHYLSCVPTSKWSC 346

[Close]

BLAST of MS018003 vs. ExPASy TrEMBL
Match: A0A6J1DW32 (RHOMBOID-like protein OS=Momordica charantia OX=3673 GN=LOC111024611 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.6e-188
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL
Sbjct: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML
Sbjct: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGNDHCSWCHYLSCVPTSKWDCGN
Sbjct: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 328

[Close]

BLAST of MS018003 vs. ExPASy TrEMBL
Match: A0A0A0KMN4 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G155500 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.1e-168
Identity = 291/328 (88.72%), Postives = 307/328 (93.60%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  NTNPN NYS SPYY+ETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSTSPYYLETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP ++N G  DKCVA+FLGRFSFQP+ ENPL GPSS+TL KLGALKW++VVH+HQGWRL
Sbjct: 61  NCP-DSNLGFGDKCVASFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIG+IYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGMIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNK AA FTLIVIV INLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKVAALFTLIVIVVINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV L+RPQFKWTERHHLPP ARRV KYKTYQYILWLAAAILLVAGFT+GLVML
Sbjct: 241 LTGFLLGFVLLVRPQFKWTERHHLPPGARRVPKYKTYQYILWLAAAILLVAGFTLGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDC N
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCAN 327

[Close]

BLAST of MS018003 vs. ExPASy TrEMBL
Match: A0A5A7TMJ5 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002940 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.9e-167
Identity = 289/328 (88.11%), Postives = 306/328 (93.29%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  NTNPN NYS+S YYIETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSSSSYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP N+N G  DKCVA FLGRFSFQP+ ENPL GPSS+TL KLGALKW++VVH+HQGWRL
Sbjct: 61  NCP-NSNLGFGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
            SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIGIIYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 FSCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAA FTLIVI+ INLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIIVINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV L+RPQFKWTERHHLPP A+RV KYKTYQYILWLAAAI+LVAGFT+GLVML
Sbjct: 241 LTGFLLGFVLLVRPQFKWTERHHLPPGAQRVPKYKTYQYILWLAAAIVLVAGFTLGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDC N
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCAN 327

[Close]

BLAST of MS018003 vs. ExPASy TrEMBL
Match: A0A1S3AU27 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103482712 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.9e-167
Identity = 289/328 (88.11%), Postives = 306/328 (93.29%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  NTNPN NYS+S YYIETSEKQWTPWL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSSSSYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP N+N G  DKCVA FLGRFSFQP+ ENPL GPSS+TL KLGALKW++VVH+HQGWRL
Sbjct: 61  NCP-NSNLGFGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
            SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIGIIYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 FSCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAA FTLIVI+ INLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIIVINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV L+RPQFKWTERHHLPP A+RV KYKTYQYILWLAAAI+LVAGFT+GLVML
Sbjct: 241 LTGFLLGFVLLVRPQFKWTERHHLPPGAQRVPKYKTYQYILWLAAAIVLVAGFTLGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDC N
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCAN 327

[Close]

BLAST of MS018003 vs. ExPASy TrEMBL
Match: A0A6J1H457 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111459437 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.5e-165
Identity = 290/328 (88.41%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETSEKQWTPWLIPMFVVANVAMFIVVMYVN 60
           MSNQDLERGAAKN  N+NP  NYSA PY++ETSEKQWT WL+PMFVVAN+AMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNSNPTGNYSAPPYHVETSEKQWTAWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWRL 120
           NCP   N G  DKCVA FLGRFSFQPL ENPL GPSS+TL KLGALKWD+VVH+HQGWRL
Sbjct: 61  NCP-KTNLGFGDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRL 120

Query: 121 LSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNIS 180
           LSCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIGIIYLVAGVGGS MSSLFI+NNIS
Sbjct: 121 LSCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIYTNKAAA FTLIVIVAINLAVGILPHVDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGF 240

Query: 241 LAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVML 300
           L GFLLGFV LIRPQFKW ERHHLPPSA+RV KYK YQYILW+ AAILLVAGFTVGLVML
Sbjct: 241 LTGFLLGFVLLIRPQFKWIERHHLPPSAQRVPKYKIYQYILWIVAAILLVAGFTVGLVML 300

Query: 301 FRGENGNDHCSWCHYLSCVPTSKWDCGN 329
           FRGENGN HCSWCHYLSCVPTS+WDCGN
Sbjct: 301 FRGENGNKHCSWCHYLSCVPTSRWDCGN 327

[Close]

BLAST of MS018003 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 449.5 bits (1155), Expect = 2.3e-126
Identity = 209/292 (71.58%), Postives = 244/292 (83.56%), Query Frame = 0


Query: 36  QWTPWLIPMFVVANVAMFIVVMYVNNCPTN-NNRGSRDKCVANFLGRFSFQPLNENPLFG 95
           +WT WL+PMFVVANVA+F+V M+VNNCP +  +   R  CVA FLGR SF+PL  NPLFG
Sbjct: 52  RWTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGRLSFEPLRTNPLFG 111

Query: 96  PSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 155
           PSS TLEKLGAL+W +VV + +GWRLL+CIWLHAG+IHL ANMLSLV IGIRLEQQFGFV
Sbjct: 112 PSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFV 171

Query: 156 RIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFT 215
           RIG+IYL++G+GGS +SSLFIRN+ISVGASGALFGLLG+MLSEL TNWTIY+NK AA  T
Sbjct: 172 RIGVIYLLSGIGGSVLSSLFIRNSISVGASGALFGLLGSMLSELFTNWTIYSNKIAALLT 231

Query: 216 LIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKY 275
           L+ ++ INLA+GILPHVDNFAH+GGF+ GFLLGF+ L RPQFKW  R H+P       KY
Sbjct: 232 LLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPLRYKY 291

Query: 276 KTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
           KTYQY+LWL + +LL+AGF V L+MLFRGENGNDHC WCHYL CVPTS W C
Sbjct: 292 KTYQYLLWLLSLVLLIAGFVVALLMLFRGENGNDHCRWCHYLRCVPTSSWRC 343

[Close]

BLAST of MS018003 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 443.0 bits (1138), Expect = 2.1e-124
Identity = 219/327 (66.97%), Postives = 260/327 (79.51%), Query Frame = 0


Query: 1   MSNQDLERGAAKNQANTNPNRNYSASPYYIETS-EKQWTPWLIPMFVVANVAMFIVVMYV 60
           M+N+D+ER   KN+     N NY    +Y E+S E  WT WLIP  VVAN+A+FI VM+V
Sbjct: 1   MANRDVERVGKKNR---GANNNY----FYEESSGETHWTSWLIPAIVVANLAVFIAVMFV 60

Query: 61  NNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFGPSSSTLEKLGALKWDRVVHQHQGWR 120
           N+CP     G   +CVA FLGRFSFQPL ENPLFGPSSSTLEK+GAL+W +VVH+HQGWR
Sbjct: 61  NDCP-KKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWR 120

Query: 121 LLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSTMSSLFIRNNI 180
           LLSC+WLHAGIIHLL NMLSL+ IGIRLEQQFGF+R+G+IYL++G+GGS +SSLF++ +I
Sbjct: 121 LLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESI 180

Query: 181 SVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFTLIVIVAINLAVGILPHVDNFAHIGG 240
           SVGASGALFGLLGAMLSELLTNWTIY NKAAA  TL+ I+AINLA+G+LP VDNFAHIGG
Sbjct: 181 SVGASGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGG 240

Query: 241 FLAGFLLGFVFLIRPQFKWTERHHLPPSARRVSKYKTYQYILWLAAAILLVAGFTVGLVM 300
           FL GF LGFV L+RPQ+ W        ++R   KY  YQY+L++ + +LLV G TV LVM
Sbjct: 241 FLTGFCLGFVLLVRPQYGWEASR--TNTSRTKRKYSMYQYVLFVVSVVLLVVGLTVALVM 300

Query: 301 LFRGENGNDHCSWCHYLSCVPTSKWDC 327
           LF+GENGN HC WCHYLSC PTSKW C
Sbjct: 301 LFKGENGNKHCKWCHYLSCFPTSKWTC 317

[Close]

BLAST of MS018003 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 408.7 bits (1049), Expect = 4.5e-114
Identity = 196/297 (65.99%), Postives = 238/297 (80.13%), Query Frame = 0


Query: 35  KQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLFG 94
           K W PWL+P  VVAN+A+F + M++NNCP N+       C+A FLGRF+FQP+ ENPL G
Sbjct: 52  KLWFPWLVPAIVVANIALFAISMFINNCPKNS-----AYCLARFLGRFAFQPMKENPLLG 111

Query: 95  PSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 154
           PSS TLEK+GAL    VVH+H+ WRL +CIWLHAG+ H+LANMLSL+ IGIRLEQ+FGFV
Sbjct: 112 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 171

Query: 155 RIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFFT 214
           RIG++Y+++G GGS +SSLF R  ISVGASGALFGLLGAMLSELLTNWTIY NK AA  T
Sbjct: 172 RIGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLT 231

Query: 215 LIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTERHHLP-----PSAR 274
           LI I+AINLAVGILPHVDNFAH+GGF +GFLLGFVFLIRPQ+ +  + + P     PSA+
Sbjct: 232 LIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAK 291

Query: 275 RVSKYKTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
             SK+K YQY+LW+ + +LL+AG+T GLV+L RG + N HCSWCHYLSC+PTS W C
Sbjct: 292 --SKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSLWSC 341

[Close]

BLAST of MS018003 vs. TAIR 10
Match: AT1G12750.1 (RHOMBOID-like protein 6 )

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-106
Identity = 185/294 (62.93%), Postives = 224/294 (76.19%), Query Frame = 0


Query: 34  EKQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLF 93
           + QWT WL P  VVANV++FIVVMY N+CP     G+   CVA  L RFSFQPL ENP  
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCP-KTTTGANGDCVAKLLRRFSFQPLRENPFL 74

Query: 94  GPSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGF 153
           GPSSSTLEKLGAL W +VV  ++ WRL++ +WLHAGIIHL+ NM  +++ GIRLEQQFGF
Sbjct: 75  GPSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGF 134

Query: 154 VRIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFF 213
           +RIG+IYL++G GGS +S+LF++ +ISVGASGAL GL+GAMLSELLTNWTIY +K  A  
Sbjct: 135 IRIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALL 194

Query: 214 TLIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTE-RHHLPPSARRVS 273
           + + I+AINLA+G+LP VDNFAHIGG L GF LGF+ L++PQ  W E R+     AR  S
Sbjct: 195 SFLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARS 254

Query: 274 KYKTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
           KY   QY+L+  AA+L+VAG TVGLVMLF GENGN HC WCH L C PTSKW C
Sbjct: 255 KYNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

[Close]

BLAST of MS018003 vs. TAIR 10
Match: AT1G12750.2 (RHOMBOID-like protein 6 )

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-106
Identity = 185/294 (62.93%), Postives = 224/294 (76.19%), Query Frame = 0


Query: 34  EKQWTPWLIPMFVVANVAMFIVVMYVNNCPTNNNRGSRDKCVANFLGRFSFQPLNENPLF 93
           + QWT WL P  VVANV++FIVVMY N+CP     G+   CVA  L RFSFQPL ENP  
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCP-KTTTGANGDCVAKLLRRFSFQPLRENPFL 74

Query: 94  GPSSSTLEKLGALKWDRVVHQHQGWRLLSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGF 153
           GPSSSTLEKLGAL W +VV  ++ WRL++ +WLHAGIIHL+ NM  +++ GIRLEQQFGF
Sbjct: 75  GPSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGF 134

Query: 154 VRIGIIYLVAGVGGSTMSSLFIRNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAAFF 213
           +RIG+IYL++G GGS +S+LF++ +ISVGASGAL GL+GAMLSELLTNWTIY +K  A  
Sbjct: 135 IRIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALL 194

Query: 214 TLIVIVAINLAVGILPHVDNFAHIGGFLAGFLLGFVFLIRPQFKWTE-RHHLPPSARRVS 273
           + + I+AINLA+G+LP VDNFAHIGG L GF LGF+ L++PQ  W E R+     AR  S
Sbjct: 195 SFLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARS 254

Query: 274 KYKTYQYILWLAAAILLVAGFTVGLVMLFRGENGNDHCSWCHYLSCVPTSKWDC 327
           KY   QY+L+  AA+L+VAG TVGLVMLF GENGN HC WCH L C PTSKW C
Sbjct: 255 KYNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

The following BLAST results are available for this feature:

BLAST of MS018003 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158027.1	3.4e-188	100.00	RHOMBOID-like protein 2 [Momordica charantia]	[more]
XP_038874691.1	1.7e-168	89.63	RHOMBOID-like protein 2 [Benincasa hispida]	[more]
XP_004143929.1	1.5e-167	88.72	RHOMBOID-like protein 2 [Cucumis sativus] >KGN50139.1 hypothetical protein Csa_0...	[more]
KAG6606117.1	7.3e-167	89.02	RHOMBOID-like protein 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7036061.1	9.5e-167	89.02	RHOMBOID-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS018003 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LYP1	3.2e-125	71.58	RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1	[more]
Q9CAN1	3.0e-123	66.97	RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1	[more]
Q0WQX7	6.3e-113	65.99	RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1	[more]
Q8VZ48	2.2e-105	62.93	RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P...	[more]
F4JBM4	5.3e-104	60.62	RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1	[more]

BLAST of MS018003 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DW32	1.6e-188	100.00	RHOMBOID-like protein OS=Momordica charantia OX=3673 GN=LOC111024611 PE=3 SV=1	[more]
A0A0A0KMN4	7.1e-168	88.72	RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G155500 PE=3 SV=1	[more]
A0A5A7TMJ5	7.9e-167	88.11	RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44...	[more]
A0A1S3AU27	7.9e-167	88.11	RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103482712 PE=3 SV=1	[more]
A0A6J1H457	1.5e-165	88.41	RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111459437 PE=3 SV=1	[more]

BLAST of MS018003 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07250.1	2.3e-126	71.58	RHOMBOID-like protein 3	[more]
AT1G63120.1	2.1e-124	66.97	RHOMBOID-like 2	[more]
AT2G29050.1	4.5e-114	65.99	RHOMBOID-like 1	[more]
AT1G12750.1	1.5e-106	62.93	RHOMBOID-like protein 6	[more]
AT1G12750.2	1.5e-106	62.93	RHOMBOID-like protein 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 112..253 e-value: 2.3E-40 score: 138.0
IPR035952	Rhomboid-like superfamily	GENE3D	1.20.1540.10		coord: 33..255 e-value: 2.3E-37 score: 130.5
IPR035952	Rhomboid-like superfamily	SUPERFAMILY	144091	Rhomboid-like	coord: 36..257
None	No IPR available	PANTHER	PTHR22936:SF93	RHOMBOID-LIKE PROTEIN	coord: 1..328
IPR002610	Peptidase S54, rhomboid	PANTHER	PTHR22936	RHOMBOID-RELATED	coord: 1..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS018003.1	MS018003.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity