MS017980 (gene) Bitter gourd (TR) v1

Overview
NameMS017980
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionoxalate--CoA ligase-like
Locationscaffold373: 4403254 .. 4405266 (+)
RNA-Seq ExpressionMS017980
SyntenyMS017980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAGTACCTTGAGTGGGTTGTTGAAGAAGGCCGCCTCTGAGTTTCCTCGCCGGCGGGCTCTCTCAGTCTCCGGAAAGTTCGACCTGACACATGAGCGCTTGCAAGAGCTCGTCGAACATGCCGCGTCTCTCCTGGCGGATGCCGGAGTTAACGCCGGCGATGTGGTCGCCCTCACCTTCCATAACACTGTGGAGGTACGTCTGCGGTGAACTTCTTGTGATTTTTTTCCTTTTTTTTTTCCGCGGATTCTCTGGCTAAGCCCCTGTTTGGTTGCTGAGAAAATTTTGGTGAGAAACAGTAGAAAAAAGTGTCTGCATAGTGTCAATTACGCAATCTTAACGTCTTTTTTGTTTTTTGTGCTTTGATTCATGAGAATGAGAGCGAGAGGATTGATTGAATTAATTGATTTACCTTTACTTTTGTAAGAAGCTGATGATGATCAAATGTTTCCTCTTGCAGTTTGTGATCATGTTTCTGGCCGTAATCCGCTGCCGAGCCACGGCTGCGCCATTAAATCCGGCTTACACGGCTGAGGAGTTCGAGTTTTACTTATCGGACTCGGAATCGAAGCTTCTCCTGACGTCAGAAGAAGGGATTCCATCCGCTCAATCCGCCGCATTGAAGCTCAATATCCCTCAAGTAAAAGCAAGCCTGTCTGGCGACGATGAGTTTCTCAATCTTTCTCCGTCAGGAACCGAATCGAAATCGAACATGGCGGAAATCGTAAACGATCCGTCCGATGCGGCGCTCTTCCTCCACACGTCTGGTACCACCAGCCGGCCGAAGGGCGTGCCCCTGACTCAACAGAACCTCGCTGCCTCTGTTCAGAACATCAAATCGGTGTACAAGATTTCCGAGTCTGACTCAACCGTACTAGTCCTTCCGTTGTTCCACGTTCACGGATTAATGGCGGGATTGCTATCGTCGCTCATCTCCGGCGCCGCCGTGACTCTTCCGGCAGCCGGTCGATTCTCTGCTTCAACGTTTTGGCCCGATATGCTCACCTATAACGCGACGTGGTACACGGCGGTCCCTACGATTCACCAAATCATACTCGATCGCCACCTCAACAAGCCGGAGGCGGCCTACCCTAAGCTCCGGTTCATCCGGAGCTGCAGCGCGTCCCTTGCACCTTCGATTATGGAGCGCCTGGAAAACTCATTCGGAGCTCCGGTTTTGGAGTCTTACGCGATGACGGAAGCGTCACATCTCATGGCGTCCAATCCGTTGCCGGAGGACGGCGTGCACAAGGCCGGATCGGTAGGGAAGCCGGTGGGCCAAGAAATGGCGATTCTGGACGAGAACGGCGTGCCGCAGTCGGAAGGCGTTAGCGGAGAAGTGTGTATAAGAGGACCAAACGTGACGAAGGGATATAAGAACAATCCAGAAGCCAATAAGGCCGCCTTCTTGTACGGATGGTTCCACACCGGCGATATCGGTTATCTCGACTCCGACGGATACTTGCATCTGGTCGGCCGGATCAAAGAGCTCATCAACCGTGGAGGTACAGTCCAACAGCTCAGATTCGAACTCAAAAAATTAAACGAATCGCCAGTGAACAAAATTCACAATTCAAATTGAAAAAATTGCATCTACAGTCATCTGATCTAATTTGCCCCTTCTTGATCCAGGTGAAAAGATCTCTCCAATTGAAGTAGATTCCGTGCTTCTGTCTCACGTCGACATTGCTCAGGCCGTCGCCTTCGGAGTCCCCGATGAAAAATACGGCGAAGAGGTATGAAAACGTACCGTTGATTATCCTCACATCCGATCGATTCCGATCAATTCACTAGTGATTTGCATTCTAACCGATGCTTCTGCAGATCAATTGCGCTATAATTCCAAGGGAGGGAAGTTCAATCGACGAGGCGGAGGTGATGCGATTTTGCAAGAAAAACCTAGCCTCCTTCAAGGTCCCGAAGAAAGTGTTCATCACCGATTCCCTACCAAAAACTGCTACCGGGAAGATCCAACGCCGAATCGTGGCGGAGCATTTTCTAACTCCGATCGCC

mRNA sequence

AAGAGTACCTTGAGTGGGTTGTTGAAGAAGGCCGCCTCTGAGTTTCCTCGCCGGCGGGCTCTCTCAGTCTCCGGAAAGTTCGACCTGACACATGAGCGCTTGCAAGAGCTCGTCGAACATGCCGCGTCTCTCCTGGCGGATGCCGGAGTTAACGCCGGCGATGTGGTCGCCCTCACCTTCCATAACACTGTGGAGTTTGTGATCATGTTTCTGGCCGTAATCCGCTGCCGAGCCACGGCTGCGCCATTAAATCCGGCTTACACGGCTGAGGAGTTCGAGTTTTACTTATCGGACTCGGAATCGAAGCTTCTCCTGACGTCAGAAGAAGGGATTCCATCCGCTCAATCCGCCGCATTGAAGCTCAATATCCCTCAAGTAAAAGCAAGCCTGTCTGGCGACGATGAGTTTCTCAATCTTTCTCCGTCAGGAACCGAATCGAAATCGAACATGGCGGAAATCGTAAACGATCCGTCCGATGCGGCGCTCTTCCTCCACACGTCTGGTACCACCAGCCGGCCGAAGGGCGTGCCCCTGACTCAACAGAACCTCGCTGCCTCTGTTCAGAACATCAAATCGGTGTACAAGATTTCCGAGTCTGACTCAACCGTACTAGTCCTTCCGTTGTTCCACGTTCACGGATTAATGGCGGGATTGCTATCGTCGCTCATCTCCGGCGCCGCCGTGACTCTTCCGGCAGCCGGTCGATTCTCTGCTTCAACGTTTTGGCCCGATATGCTCACCTATAACGCGACGTGGTACACGGCGGTCCCTACGATTCACCAAATCATACTCGATCGCCACCTCAACAAGCCGGAGGCGGCCTACCCTAAGCTCCGGTTCATCCGGAGCTGCAGCGCGTCCCTTGCACCTTCGATTATGGAGCGCCTGGAAAACTCATTCGGAGCTCCGGTTTTGGAGTCTTACGCGATGACGGAAGCGTCACATCTCATGGCGTCCAATCCGTTGCCGGAGGACGGCGTGCACAAGGCCGGATCGGTAGGGAAGCCGGTGGGCCAAGAAATGGCGATTCTGGACGAGAACGGCGTGCCGCAGTCGGAAGGCGTTAGCGGAGAAGTGTGTATAAGAGGACCAAACGTGACGAAGGGATATAAGAACAATCCAGAAGCCAATAAGGCCGCCTTCTTGTACGGATGGTTCCACACCGGCGATATCGGTTATCTCGACTCCGACGGATACTTGCATCTGGTCGGCCGGATCAAAGAGCTCATCAACCGTGGAGGTGAAAAGATCTCTCCAATTGAAGTAGATTCCGTGCTTCTGTCTCACGTCGACATTGCTCAGGCCGTCGCCTTCGGAGTCCCCGATGAAAAATACGGCGAAGAGATCAATTGCGCTATAATTCCAAGGGAGGGAAGTTCAATCGACGAGGCGGAGGTGATGCGATTTTGCAAGAAAAACCTAGCCTCCTTCAAGGTCCCGAAGAAAGTGTTCATCACCGATTCCCTACCAAAAACTGCTACCGGGAAGATCCAACGCCGAATCGTGGCGGAGCATTTTCTAACTCCGATCGCC

Coding sequence (CDS)

AAGAGTACCTTGAGTGGGTTGTTGAAGAAGGCCGCCTCTGAGTTTCCTCGCCGGCGGGCTCTCTCAGTCTCCGGAAAGTTCGACCTGACACATGAGCGCTTGCAAGAGCTCGTCGAACATGCCGCGTCTCTCCTGGCGGATGCCGGAGTTAACGCCGGCGATGTGGTCGCCCTCACCTTCCATAACACTGTGGAGTTTGTGATCATGTTTCTGGCCGTAATCCGCTGCCGAGCCACGGCTGCGCCATTAAATCCGGCTTACACGGCTGAGGAGTTCGAGTTTTACTTATCGGACTCGGAATCGAAGCTTCTCCTGACGTCAGAAGAAGGGATTCCATCCGCTCAATCCGCCGCATTGAAGCTCAATATCCCTCAAGTAAAAGCAAGCCTGTCTGGCGACGATGAGTTTCTCAATCTTTCTCCGTCAGGAACCGAATCGAAATCGAACATGGCGGAAATCGTAAACGATCCGTCCGATGCGGCGCTCTTCCTCCACACGTCTGGTACCACCAGCCGGCCGAAGGGCGTGCCCCTGACTCAACAGAACCTCGCTGCCTCTGTTCAGAACATCAAATCGGTGTACAAGATTTCCGAGTCTGACTCAACCGTACTAGTCCTTCCGTTGTTCCACGTTCACGGATTAATGGCGGGATTGCTATCGTCGCTCATCTCCGGCGCCGCCGTGACTCTTCCGGCAGCCGGTCGATTCTCTGCTTCAACGTTTTGGCCCGATATGCTCACCTATAACGCGACGTGGTACACGGCGGTCCCTACGATTCACCAAATCATACTCGATCGCCACCTCAACAAGCCGGAGGCGGCCTACCCTAAGCTCCGGTTCATCCGGAGCTGCAGCGCGTCCCTTGCACCTTCGATTATGGAGCGCCTGGAAAACTCATTCGGAGCTCCGGTTTTGGAGTCTTACGCGATGACGGAAGCGTCACATCTCATGGCGTCCAATCCGTTGCCGGAGGACGGCGTGCACAAGGCCGGATCGGTAGGGAAGCCGGTGGGCCAAGAAATGGCGATTCTGGACGAGAACGGCGTGCCGCAGTCGGAAGGCGTTAGCGGAGAAGTGTGTATAAGAGGACCAAACGTGACGAAGGGATATAAGAACAATCCAGAAGCCAATAAGGCCGCCTTCTTGTACGGATGGTTCCACACCGGCGATATCGGTTATCTCGACTCCGACGGATACTTGCATCTGGTCGGCCGGATCAAAGAGCTCATCAACCGTGGAGGTGAAAAGATCTCTCCAATTGAAGTAGATTCCGTGCTTCTGTCTCACGTCGACATTGCTCAGGCCGTCGCCTTCGGAGTCCCCGATGAAAAATACGGCGAAGAGATCAATTGCGCTATAATTCCAAGGGAGGGAAGTTCAATCGACGAGGCGGAGGTGATGCGATTTTGCAAGAAAAACCTAGCCTCCTTCAAGGTCCCGAAGAAAGTGTTCATCACCGATTCCCTACCAAAAACTGCTACCGGGAAGATCCAACGCCGAATCGTGGCGGAGCATTTTCTAACTCCGATCGCC

Protein sequence

KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKLNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCIRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKKVFITDSLPKTATGKIQRRIVAEHFLTPIA
Homology
BLAST of MS017980 vs. NCBI nr
Match: XP_022159678.1 (oxalate--CoA ligase-like [Momordica charantia])

HSP 1 Score: 990.7 bits (2560), Expect = 4.6e-285
Identity = 508/511 (99.41%), Postives = 509/511 (99.61%), Query Frame = 0

Query: 1   KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTF 60
           KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTF
Sbjct: 3   KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTF 62

Query: 61  HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALK 120
           HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESK LLTSEEGIPSAQSAALK
Sbjct: 63  HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAALK 122

Query: 121 LNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ 180
           LNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ
Sbjct: 123 LNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ 182

Query: 181 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 240
           QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST
Sbjct: 183 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 242

Query: 241 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 300
           FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF
Sbjct: 243 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 302

Query: 301 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVC 360
           GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILD NGVPQSEGVSGEVC
Sbjct: 303 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGEVC 362

Query: 361 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 420
           IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI
Sbjct: 363 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 422

Query: 421 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 480
           EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP
Sbjct: 423 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 482

Query: 481 KKVFITDSLPKTATGKIQRRIVAEHFLTPIA 512
           K+VFITDSLPKTATGKIQRRIVAEHFLTPIA
Sbjct: 483 KRVFITDSLPKTATGKIQRRIVAEHFLTPIA 513

BLAST of MS017980 vs. NCBI nr
Match: KAG7036051.1 (Oxalate--CoA ligase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 888.6 bits (2295), Expect = 2.5e-254
Identity = 449/507 (88.56%), Postives = 475/507 (93.69%), Query Frame = 0

Query: 2   STLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 61
           STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVALTF 
Sbjct: 4   STLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVALTFP 63

Query: 62  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 121
           NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGIPSAQ+AA KL
Sbjct: 64  NTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAASKL 123

Query: 122 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 181
           NIPQV  +L+  D FL+  PS  ESK NM+EIVNDPSDAALFLHTSGTTSRPKGVPLTQQ
Sbjct: 124 NIPQVNVNLASGDAFLDFFPSPNESKVNMSEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 183

Query: 182 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 241
           NLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF
Sbjct: 184 NLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 243

Query: 242 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFG 301
           W DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFG
Sbjct: 244 WSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEKSFG 303

Query: 302 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCI 361
           APVLESYAMTEASHLMASNPLP+DGVHKAGSVGKP+GQEMAILDENGV Q EGV GEVCI
Sbjct: 304 APVLESYAMTEASHLMASNPLPDDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGEVCI 363

Query: 362 RGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIE 421
           RGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 364 RGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 423

Query: 422 VDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPK 481
           VD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK+PK
Sbjct: 424 VDAVLLSHADIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFKIPK 483

Query: 482 KVFITDSLPKTATGKIQRRIVAEHFLT 509
           KVFITD LPKTA+GKIQRR VAEHFL+
Sbjct: 484 KVFITDFLPKTASGKIQRRFVAEHFLS 510

BLAST of MS017980 vs. NCBI nr
Match: XP_038874771.1 (oxalate--CoA ligase-like [Benincasa hispida])

HSP 1 Score: 888.3 bits (2294), Expect = 3.2e-254
Identity = 448/505 (88.71%), Postives = 477/505 (94.46%), Query Frame = 0

Query: 3   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHN 62
           TL+GLLKKAASEFPRRRALSVSGKFDLTHERLQELV HAA  L D GV AGDVVALTF N
Sbjct: 5   TLTGLLKKAASEFPRRRALSVSGKFDLTHERLQELVVHAAGCLVDVGVKAGDVVALTFPN 64

Query: 63  TVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKLN 122
           TVE+VIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLL+TS+EGIPSAQ+AA KLN
Sbjct: 65  TVEYVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLITSKEGIPSAQTAATKLN 124

Query: 123 IPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN 182
           IPQVKA+LS  +EF++L PS TE+KSN+AEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN
Sbjct: 125 IPQVKANLSSGNEFIDLFPSATETKSNIAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN 184

Query: 183 LAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFW 242
           LAASVQNIKSVYK SESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSASTFW
Sbjct: 185 LAASVQNIKSVYKFSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSASTFW 244

Query: 243 PDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGA 302
            DM+ YNATWYTAVPTIHQIILDRH +KPEA+YPKLRFIRSCSASLAPS++ERLE S GA
Sbjct: 245 SDMIAYNATWYTAVPTIHQIILDRHFSKPEASYPKLRFIRSCSASLAPSVLERLETSLGA 304

Query: 303 PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCIR 362
           PVLE+YAMTEASHLMASNPLPEDG HKAGSVGKP+GQEMAILDENGV Q EGV GEVCIR
Sbjct: 305 PVLEAYAMTEASHLMASNPLPEDGGHKAGSVGKPIGQEMAILDENGVMQPEGVKGEVCIR 364

Query: 363 GPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEV 422
           G NVTKGYKNNPEANKAAF++GWFHTGD+G+ DSDGYLHLVGRIKELINRGGEKISPIEV
Sbjct: 365 GANVTKGYKNNPEANKAAFMFGWFHTGDVGFFDSDGYLHLVGRIKELINRGGEKISPIEV 424

Query: 423 DSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKK 482
           D+VLLSH +IAQAVAFGVPDEKYGEEINCAIIPR GSSIDEA+V++FCKKNLASFKVPKK
Sbjct: 425 DAVLLSHANIAQAVAFGVPDEKYGEEINCAIIPRGGSSIDEADVLQFCKKNLASFKVPKK 484

Query: 483 VFITDSLPKTATGKIQRRIVAEHFL 508
           VFITD LPKTA+GKIQRR VAEHFL
Sbjct: 485 VFITDYLPKTASGKIQRRFVAEHFL 509

BLAST of MS017980 vs. NCBI nr
Match: KAG6606105.1 (Oxalate--CoA ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887.5 bits (2292), Expect = 5.5e-254
Identity = 449/505 (88.91%), Postives = 473/505 (93.66%), Query Frame = 0

Query: 2   STLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 61
           STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVALTF 
Sbjct: 33  STLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVALTFP 92

Query: 62  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 121
           NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGIPSAQ+AA KL
Sbjct: 93  NTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAASKL 152

Query: 122 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 181
           NIPQV  +L+  D FL+  PS  ESK NM+EIVNDPSDAALFLHTSGTTSRPKGVPLTQQ
Sbjct: 153 NIPQVNVNLASGDAFLDFFPSPNESKVNMSEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 212

Query: 182 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 241
           NLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF
Sbjct: 213 NLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 272

Query: 242 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFG 301
           W DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFG
Sbjct: 273 WSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEKSFG 332

Query: 302 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCI 361
           APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILDENGV Q EGV GEVCI
Sbjct: 333 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGEVCI 392

Query: 362 RGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIE 421
           RGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 393 RGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 452

Query: 422 VDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPK 481
           VD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK+PK
Sbjct: 453 VDAVLLSHSDIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFKIPK 512

Query: 482 KVFITDSLPKTATGKIQRRIVAEHF 507
           KVFITD LPKTA+GKIQRR VAEHF
Sbjct: 513 KVFITDFLPKTASGKIQRRFVAEHF 537

BLAST of MS017980 vs. NCBI nr
Match: XP_023534085.1 (oxalate--CoA ligase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.1 bits (2291), Expect = 7.2e-254
Identity = 448/507 (88.36%), Postives = 475/507 (93.69%), Query Frame = 0

Query: 2   STLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 61
           STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVALTF 
Sbjct: 4   STLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVALTFP 63

Query: 62  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 121
           NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGIPSAQ+AA KL
Sbjct: 64  NTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAASKL 123

Query: 122 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 181
           NIPQV  +L+  D FL+  PS  ES+ NM+EIVNDPSDAALFLHTSGTTSRPKGVPLTQQ
Sbjct: 124 NIPQVNVNLASGDAFLDFFPSPNESEVNMSEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 183

Query: 182 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 241
           NLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF
Sbjct: 184 NLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 243

Query: 242 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFG 301
           W DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFG
Sbjct: 244 WSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEKSFG 303

Query: 302 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCI 361
           APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILDENGV Q EGV GEVCI
Sbjct: 304 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGEVCI 363

Query: 362 RGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIE 421
           RGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 364 RGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 423

Query: 422 VDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPK 481
           VD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK+PK
Sbjct: 424 VDAVLLSHADIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFKIPK 483

Query: 482 KVFITDSLPKTATGKIQRRIVAEHFLT 509
           KVFITD LPKTA+GKIQRR VAEHF++
Sbjct: 484 KVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MS017980 vs. ExPASy Swiss-Prot
Match: M4IQQ7 (Probable CoA ligase CCL9 OS=Humulus lupulus OX=3486 GN=CCL9 PE=2 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 3.3e-233
Identity = 410/511 (80.23%), Postives = 452/511 (88.45%), Query Frame = 0

Query: 3   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHN 62
           TL+GLLKKAA+EF  RRA+ VSG+F LTH RLQE+V+HAASLL  +GV  GDVVALTF N
Sbjct: 6   TLTGLLKKAAAEFSDRRAILVSGEFHLTHARLQEIVDHAASLLLASGVGHGDVVALTFPN 65

Query: 63  TVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKLN 122
           T+E+++MFLAVIRCRA AAPLN AYTAEEFEFYLSDSESKLL+T  +GI +A++AA KLN
Sbjct: 66  TIEYIVMFLAVIRCRAVAAPLNSAYTAEEFEFYLSDSESKLLITPPKGIEAARAAASKLN 125

Query: 123 IPQVKASLSGDDEFLNLSPSGTESKS--NMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ 182
           I  V A+L G D  + LSPS     S   +AE+ NDPSD +LFLHTSGTTSRPKGVPLTQ
Sbjct: 126 ITHVTATLPGGDGLIALSPSPNNESSLDAVAELTNDPSDVSLFLHTSGTTSRPKGVPLTQ 185

Query: 183 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 242
            NLA+SVQNIKSVYK+SESDSTV+VLPLFHVHG++AGLLSSLI+G+AVTLPAAGRFSAST
Sbjct: 186 LNLASSVQNIKSVYKLSESDSTVVVLPLFHVHGMIAGLLSSLIAGSAVTLPAAGRFSAST 245

Query: 243 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 302
           FW DM+  NATWYTAVPTIHQIILDRHLNKPE  YPKLRFIRSCSASLAP+IM RLE SF
Sbjct: 246 FWSDMIACNATWYTAVPTIHQIILDRHLNKPEPTYPKLRFIRSCSASLAPTIMGRLEESF 305

Query: 303 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVC 362
           GAPVLE+YAMTEA+HLMASNPLPEDG HK GSVGKPVGQEMAILD NG  Q  G SGEVC
Sbjct: 306 GAPVLEAYAMTEAAHLMASNPLPEDGGHKPGSVGKPVGQEMAILDLNGSAQLPGFSGEVC 365

Query: 363 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 422
           IRGPNVTKGYKNNPEANKAAF +GWFHTGD+GY D DGYLHLVGRIKELINRGGEKISPI
Sbjct: 366 IRGPNVTKGYKNNPEANKAAFQFGWFHTGDVGYFDEDGYLHLVGRIKELINRGGEKISPI 425

Query: 423 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 482
           EVD+VLLSH D+AQAVAFGVPD+KYGEEINCA+IPREGS +DE  V+RFCKKNLA+FKVP
Sbjct: 426 EVDAVLLSHPDLAQAVAFGVPDDKYGEEINCAVIPREGSEVDEDGVLRFCKKNLAAFKVP 485

Query: 483 KKVFITDSLPKTATGKIQRRIVAEHFLTPIA 512
           KKVFITDSLPKTATGKIQRRIVAEHFL  I+
Sbjct: 486 KKVFITDSLPKTATGKIQRRIVAEHFLAQIS 516

BLAST of MS017980 vs. ExPASy Swiss-Prot
Match: Q9SMT7 (Oxalate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE3 PE=1 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 6.0e-219
Identity = 389/507 (76.73%), Postives = 439/507 (86.59%), Query Frame = 0

Query: 3   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAAS-LLADAGVNAGDVVALTFH 62
           TLSGLL+  A +FP RRALSVSGKF+LTH RL +L+E AAS L++DAG+  GDVVALTF 
Sbjct: 5   TLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVALTFP 64

Query: 63  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 122
           NTVEFVIMFLAVIR RATAAPLN AYTAEEFEFYLSDS+SKLLLTS+EG   AQ AA KL
Sbjct: 65  NTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEAASKL 124

Query: 123 NIPQVKASLSGDDEFLNLSPSGTESKSNMA-EIVNDPSDAALFLHTSGTTSRPKGVPLTQ 182
            I  V A+L      L LS + ++S  + A E+VN P D ALFLHTSGTTSRPKGVPLTQ
Sbjct: 125 KISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGVPLTQ 184

Query: 183 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 242
            NLA+SV+NIK+VYK++ESDSTV+VLPLFHVHGL+AGLLSSL +GAAVTLPAAGRFSA+T
Sbjct: 185 LNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRFSATT 244

Query: 243 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 302
           FWPDM  YNATWYTAVPTIHQIILDRH + PE  YPKLRFIRSCSASLAP I+ RLE +F
Sbjct: 245 FWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRLEEAF 304

Query: 303 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVC 362
           GAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKPVGQEMAIL+E G  Q     GEVC
Sbjct: 305 GAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNKGEVC 364

Query: 363 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 422
           IRGPNVTKGYKNNPEANKA F +GWFHTGDIGY D+DGYLHLVGRIKELINRGGEKISPI
Sbjct: 365 IRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEKISPI 424

Query: 423 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 482
           EVD+VLL+H D++Q VAFGVPDEKYGEEINCA+IPREG+++ E ++  FCKKNLA+FKVP
Sbjct: 425 EVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAAFKVP 484

Query: 483 KKVFITDSLPKTATGKIQRRIVAEHFL 508
           K+VFITD+LPKTA+GKIQRRIVA+HFL
Sbjct: 485 KRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of MS017980 vs. ExPASy Swiss-Prot
Match: O74976 (Oxalate--CoA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pcs60 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 9.8e-121
Identity = 232/494 (46.96%), Postives = 318/494 (64.37%), Query Frame = 0

Query: 18  RRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCR 77
           R  ++ S   +L+   L+  +      +A  G+  GD V +   N +EFV+ F AV   R
Sbjct: 17  RALVAPSLNAELSFSELRIAIMDLQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQR 76

Query: 78  ATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPS---AQSAALKLNIPQVKASLSGDD 137
           A   PLN  Y   EFEFY+ D +SKL++  E  + +   A  AA KL++   + +     
Sbjct: 77  AICGPLNSNYKQSEFEFYIDDLKSKLVIVPEGSVAANTPAVRAAKKLSVAVAELAWCPKS 136

Query: 138 EFLNLSP-SGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSV 197
             + +    G +  +     +  P D  L LHTSGTT RPK VPLT +NL  S+ NI + 
Sbjct: 137 RLVRIVHFEGAKINAPQPLGLPQPDDVMLVLHTSGTTGRPKVVPLTHKNLCRSIHNITTS 196

Query: 198 YKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWY 257
           Y++   D++ +V+PLFHVHGL+ GLLS+L SG    +P   +FSA +FW + + Y ATWY
Sbjct: 197 YRLDPRDTSYVVMPLFHVHGLLCGLLSTLASGGCAVVPP--KFSAHSFWKEFIQYGATWY 256

Query: 258 TAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGAPVLESYAMTEA 317
           TAVPTIHQI+L     KP    P++RFIRSCS+ LAP ++ +LE +F APVLE+YAMTEA
Sbjct: 257 TAVPTIHQILLRTPPPKP---LPRIRFIRSCSSPLAPPVLSKLEATFRAPVLEAYAMTEA 316

Query: 318 SHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCIRGPNVTKGYKNN 377
           SH M +NPLP   VHK  SVGKP G E+ ILD+ G    +G  GE+C+RG NVTKGY NN
Sbjct: 317 SHQMTTNPLP-PLVHKPHSVGKPFGVELKILDQKGNEMPQGKEGEICVRGINVTKGYLNN 376

Query: 378 PEANKAAFLYG-WFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDI 437
           P ANK++F    +F TGD G LD DGY+ + GRIKEL+NRGGEKISP E+D+VL+ H D+
Sbjct: 377 PAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRIKELVNRGGEKISPAEIDAVLMQHPDV 436

Query: 438 AQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKKVFITDSLPKT 497
           ++AV F VPDEKYG++I  AI P  G ++   ++  + ++ +A+FK+PKK + TD +PKT
Sbjct: 437 SEAVCFAVPDEKYGQDIQAAINPVAGKTVTPKQLHDYLEQKVAAFKIPKKFYFTDRIPKT 496

Query: 498 ATGKIQRRIVAEHF 507
           ATGK+QRR+V + F
Sbjct: 497 ATGKVQRRLVCDAF 504

BLAST of MS017980 vs. ExPASy Swiss-Prot
Match: P38137 (Oxalate--CoA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PCS60 PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 7.5e-105
Identity = 227/512 (44.34%), Postives = 302/512 (58.98%), Query Frame = 0

Query: 29  LTHERLQELVEHAASLLAD------AGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAP 88
           +T+  L  +V H  ++  +        V   D VA++  N +EF++ FL         AP
Sbjct: 32  VTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAP 91

Query: 89  LNPAYTAEEFEFYLSDSESKLL--------LTSEEGIPSAQS-----AALKLNIPQVKAS 148
           LNP Y  +EF FYL+D +SK +        L S E + SA +       L  +  + +  
Sbjct: 92  LNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVE 151

Query: 149 ---LSGDDEFLNLSPSGTESKSNMAEIVND----------PSDAALFLHTSGTTSRPKGV 208
               S +D +  +         N A+ VN            SD AL LHTSGTTS PK V
Sbjct: 152 YDIYSPEDNYKRV----IYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTV 211

Query: 209 PLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRF 268
           PL   N+  S  NI + YK++  D + +V+PLFHVHGL+  LLS+  +  +V +P    F
Sbjct: 212 PLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDG--F 271

Query: 269 SASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERL 328
               FW   + YN  W++ VPTI  I+L  ++ KP   +P +RFIRSCS++LAP+   +L
Sbjct: 272 HPKLFWDQFVKYNCNWFSCVPTISMIML--NMPKPN-PFPHIRFIRSCSSALAPATFHKL 331

Query: 329 ENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVS 388
           E  F APVLE+YAMTEASH M SN LP  G  K G+VG+P G  + ILD+N      G  
Sbjct: 332 EKEFNAPVLEAYAMTEASHQMTSNNLP-PGKRKPGTVGQPQGVTVVILDDNDNVLPPGKV 391

Query: 389 GEVCIRGPNVTKGYKNNPEANKAAFL--YGWFHTGDIGYLDSDGYLHLVGRIKELINRGG 448
           GEV IRG NVT GY NNP+ANK  F     +F TGD GY D +G+L L GRIKELINRGG
Sbjct: 392 GEVSIRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGG 451

Query: 449 EKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNL 507
           EKISPIE+D ++LSH  I +AVAFGVPD+ YG+ +  AI+ ++G  +   E++ F KK+L
Sbjct: 452 EKISPIELDGIMLSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHL 511

BLAST of MS017980 vs. ExPASy Swiss-Prot
Match: Q0K844 (Probable sulfoacetate--CoA ligase OS=Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) OX=381666 GN=sauT PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.3e-74
Identity = 170/506 (33.60%), Postives = 262/506 (51.78%), Query Frame = 0

Query: 3   TLSGLLKKAASEFP-RRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 62
           TL+ L+   A+++P +   LS      LT   L           A AG+ +G  V++   
Sbjct: 12  TLAALIAVRAAQWPDKPYLLSPDSGHALTFGALATDAGTLGRSYAAAGLGSGQTVSVYLP 71

Query: 63  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 122
           N  +   + L  + C     P+N      +  + L+ S+++L+ T  +G  + + A  + 
Sbjct: 72  NGEQTARLLLGTMACGLVVNPINLLCQPAQLRYILAHSDTRLVFTWPDGEAAIREALREA 131

Query: 123 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 182
            +       + D   L   P+  ++ S +      P   AL ++TSGTT  PKGV LTQ+
Sbjct: 132 GLDVPVLVTAPDANSLPALPATHDAASPLPP--PQPDAPALLMYTSGTTGTPKGVLLTQR 191

Query: 183 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 242
           NL A+  N+   + +  +D  +  LPL+H++GL+   ++ L+ G +V +P   RFSAS F
Sbjct: 192 NLVANGTNVSREHCLGPADRVLATLPLYHINGLVVTAIAPLVHGGSVVMPM--RFSASAF 251

Query: 243 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPK-LRFIRSCSASLAPSIMERLENSF 302
           W D   +  TW   VPTI   +    LN P    P  +RF RS SA+L P      E  F
Sbjct: 252 WQDSARHGCTWLNVVPTIIAYL----LNDPHGQAPAGVRFCRSASAALPPEHHRAFEARF 311

Query: 303 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVC 362
           G  V+E+  MTE +    SNPL + G  + GS+G+P G    +L  +G P  +G  GE+ 
Sbjct: 312 GIGVIETMGMTETAAPAFSNPL-DPGQRRIGSIGRPSGTRARVLGRDGKPAPDGQVGEIV 371

Query: 363 IRGPNVTKGYKNNPEANKAAFLY-GWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISP 422
           ++G +V  GY   P+  + AF + GW  TGD+GY D+DGY ++ GR KELI +GGE I+P
Sbjct: 372 LQGESVMAGYYKAPDITREAFTHDGWLRTGDLGYRDADGYFYISGRAKELIIKGGENIAP 431

Query: 423 IEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKV 482
            E+D  LL H  + +A A GVPD  YG+EI   ++ RE +  D+A +   C + L  +K 
Sbjct: 432 REIDEALLRHPGVLEAAAVGVPDPAYGQEIVAYVVMREAARCDDAALRAHCLRELGRYKT 491

Query: 483 PKKVFITDSLPKTATGKIQRRIVAEH 506
           PK+      LP+  +GK+QR  +  H
Sbjct: 492 PKEFRFIAELPRGPSGKVQRLKLLNH 508

BLAST of MS017980 vs. ExPASy TrEMBL
Match: A0A6J1DZI2 (oxalate--CoA ligase-like OS=Momordica charantia OX=3673 GN=LOC111026021 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.2e-285
Identity = 508/511 (99.41%), Postives = 509/511 (99.61%), Query Frame = 0

Query: 1   KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTF 60
           KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTF
Sbjct: 3   KSTLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTF 62

Query: 61  HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALK 120
           HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESK LLTSEEGIPSAQSAALK
Sbjct: 63  HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKFLLTSEEGIPSAQSAALK 122

Query: 121 LNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ 180
           LNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ
Sbjct: 123 LNIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQ 182

Query: 181 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 240
           QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST
Sbjct: 183 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 242

Query: 241 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 300
           FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF
Sbjct: 243 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 302

Query: 301 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVC 360
           GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILD NGVPQSEGVSGEVC
Sbjct: 303 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDGNGVPQSEGVSGEVC 362

Query: 361 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 420
           IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI
Sbjct: 363 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 422

Query: 421 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 480
           EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP
Sbjct: 423 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 482

Query: 481 KKVFITDSLPKTATGKIQRRIVAEHFLTPIA 512
           K+VFITDSLPKTATGKIQRRIVAEHFLTPIA
Sbjct: 483 KRVFITDSLPKTATGKIQRRIVAEHFLTPIA 513

BLAST of MS017980 vs. ExPASy TrEMBL
Match: A0A5A7THK1 (Oxalate--CoA ligase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002700 PE=4 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.9e-254
Identity = 447/505 (88.51%), Postives = 476/505 (94.26%), Query Frame = 0

Query: 3   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHN 62
           TL+GLLKKAASEFP RRALSVSGKFDLTHERLQEL+EHAA+ L D+GVNAGDVVALTF N
Sbjct: 5   TLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVALTFPN 64

Query: 63  TVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKLN 122
           TVE+VIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGI SAQ+AA KLN
Sbjct: 65  TVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLN 124

Query: 123 IPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN 182
           IPQVK SLS  DE++ L PS  E  SN+ EIVNDPSDAALFLHTSGTTSRPKGVPLTQQN
Sbjct: 125 IPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN 184

Query: 183 LAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFW 242
           LAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA+TFW
Sbjct: 185 LAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSATTFW 244

Query: 243 PDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGA 302
            DM+ YNATWYTAVPTIHQIIL+RH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFGA
Sbjct: 245 SDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQSFGA 304

Query: 303 PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCIR 362
           PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILDENG  QSEGV GEVCIR
Sbjct: 305 PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGEVCIR 364

Query: 363 GPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEV 422
           GPNVTKGYKNNP+ANK+AF++GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIEV
Sbjct: 365 GPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEV 424

Query: 423 DSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKK 482
           D+VLLSH DI+QAVAFGVPD KYGEEINCAIIPREGSSI+EA+V+ FCKKNLASFKVPKK
Sbjct: 425 DAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFKVPKK 484

Query: 483 VFITDSLPKTATGKIQRRIVAEHFL 508
           VFITDSLPKTATGKIQRRIVAEHFL
Sbjct: 485 VFITDSLPKTATGKIQRRIVAEHFL 509

BLAST of MS017980 vs. ExPASy TrEMBL
Match: A0A6J1H1B7 (oxalate--CoA ligase-like OS=Cucurbita moschata OX=3662 GN=LOC111459244 PE=4 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.9e-254
Identity = 448/507 (88.36%), Postives = 474/507 (93.49%), Query Frame = 0

Query: 2   STLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 61
           STL+GLLKKAAS+FP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVV LTF 
Sbjct: 4   STLTGLLKKAASQFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVTLTFP 63

Query: 62  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 121
           NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGIPSAQ+AA KL
Sbjct: 64  NTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAASKL 123

Query: 122 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 181
           NIPQV  +L+  D FL+  PS  ESK NM+EIVNDPSDAALFLHTSGTTSRPKGVPLTQQ
Sbjct: 124 NIPQVNVNLASGDAFLDFFPSPNESKVNMSEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 183

Query: 182 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 241
           NLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF
Sbjct: 184 NLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 243

Query: 242 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFG 301
           W DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFG
Sbjct: 244 WSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEKSFG 303

Query: 302 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCI 361
           APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILDENGV Q EGV GEVCI
Sbjct: 304 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGEVCI 363

Query: 362 RGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIE 421
           RGPNVTKGYKNNPEANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 364 RGPNVTKGYKNNPEANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 423

Query: 422 VDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPK 481
           VD+VLLSH DIAQAVAFGVPDEKYGEEINCA+IPREGSSIDEAEV+RFCKKNLASFK+PK
Sbjct: 424 VDAVLLSHSDIAQAVAFGVPDEKYGEEINCAVIPREGSSIDEAEVLRFCKKNLASFKIPK 483

Query: 482 KVFITDSLPKTATGKIQRRIVAEHFLT 509
           KVFITD LPKTA+GKIQRR VAEHF++
Sbjct: 484 KVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MS017980 vs. ExPASy TrEMBL
Match: A0A6J1K3G7 (oxalate--CoA ligase-like OS=Cucurbita maxima OX=3661 GN=LOC111490818 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.7e-254
Identity = 448/507 (88.36%), Postives = 475/507 (93.69%), Query Frame = 0

Query: 2   STLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFH 61
           STL+GLLKKAASEFP RRALSV+GK DLTHERLQEL+EHAAS L DAGVNAGDVVALTF 
Sbjct: 4   STLTGLLKKAASEFPHRRALSVAGKLDLTHERLQELIEHAASRLVDAGVNAGDVVALTFP 63

Query: 62  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 121
           NTVEFVI FLA+IRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGIPSAQ+AA KL
Sbjct: 64  NTVEFVITFLAIIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGIPSAQTAASKL 123

Query: 122 NIPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 181
           NIPQV  +L+  D FL+  P+ +ESK NMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ
Sbjct: 124 NIPQVNVNLASGDAFLDFFPAPSESKVNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQ 183

Query: 182 NLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 241
           NLAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF
Sbjct: 184 NLAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTF 243

Query: 242 WPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFG 301
           W DM+ YNATWYTAVPTIHQIILDRH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFG
Sbjct: 244 WSDMVAYNATWYTAVPTIHQIILDRHFSKPEPSYPKLRFIRSCSASLAPSILERLEKSFG 303

Query: 302 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCI 361
           APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILDENGV Q EGV GEVCI
Sbjct: 304 APVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGVIQPEGVKGEVCI 363

Query: 362 RGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIE 421
           RGPNVTKGYKNNP+ANKA F +GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIE
Sbjct: 364 RGPNVTKGYKNNPDANKATFSFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIE 423

Query: 422 VDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPK 481
           +D+VLLSH DIAQAVAFGV DEKYGEEINCAIIPREGSSIDEAEV+RFCKKNLASFK+PK
Sbjct: 424 IDAVLLSHADIAQAVAFGVTDEKYGEEINCAIIPREGSSIDEAEVLRFCKKNLASFKIPK 483

Query: 482 KVFITDSLPKTATGKIQRRIVAEHFLT 509
           KVFITD LPKTA+GKIQRR VAEHF++
Sbjct: 484 KVFITDFLPKTASGKIQRRFVAEHFVS 510

BLAST of MS017980 vs. ExPASy TrEMBL
Match: A0A1S3AT78 (oxalate--CoA ligase-like OS=Cucumis melo OX=3656 GN=LOC103482735 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 7.7e-254
Identity = 447/505 (88.51%), Postives = 476/505 (94.26%), Query Frame = 0

Query: 3   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAASLLADAGVNAGDVVALTFHN 62
           TL+GLLKKAASEFP RRALSVSGKFDLTHERLQEL+EHAA+ L D+GVNAGDVVALTF N
Sbjct: 5   TLTGLLKKAASEFPHRRALSVSGKFDLTHERLQELIEHAAARLVDSGVNAGDVVALTFLN 64

Query: 63  TVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKLN 122
           TVE+VIMFLAVIRCRATAAPLN AYTAEEFEFYLSDSESKLLLTS+EGI SAQ+AA KLN
Sbjct: 65  TVEYVIMFLAVIRCRATAAPLNSAYTAEEFEFYLSDSESKLLLTSKEGISSAQTAATKLN 124

Query: 123 IPQVKASLSGDDEFLNLSPSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN 182
           IPQVK SLS  DE++ L PS  E  SN+ EIVNDPSDAALFLHTSGTTSRPKGVPLTQQN
Sbjct: 125 IPQVKVSLSSGDEYIELFPSPIEMSSNVVEIVNDPSDAALFLHTSGTTSRPKGVPLTQQN 184

Query: 183 LAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFW 242
           LAASVQNIKSVYK+SESDSTVLVLPLFHVHGLMAGLLSSLI+GAAVTLPAAGRFSA+TFW
Sbjct: 185 LAASVQNIKSVYKLSESDSTVLVLPLFHVHGLMAGLLSSLIAGAAVTLPAAGRFSATTFW 244

Query: 243 PDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGA 302
            DM+ YNATWYTAVPTIHQIIL+RH +KPE +YPKLRFIRSCSASLAPSI+ERLE SFGA
Sbjct: 245 SDMIAYNATWYTAVPTIHQIILERHFSKPEPSYPKLRFIRSCSASLAPSILERLEQSFGA 304

Query: 303 PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVCIR 362
           PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKP+GQEMAILDENG  QSEGV GEVCIR
Sbjct: 305 PVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPIGQEMAILDENGAIQSEGVKGEVCIR 364

Query: 363 GPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEV 422
           GPNVTKGYKNNP+ANK+AF++GWFHTGDIG+ DSDGYLHLVGRIKELINRGGEKISPIEV
Sbjct: 365 GPNVTKGYKNNPDANKSAFMFGWFHTGDIGFFDSDGYLHLVGRIKELINRGGEKISPIEV 424

Query: 423 DSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKK 482
           D+VLLSH DI+QAVAFGVPD KYGEEINCAIIPREGSSI+EA+V+ FCKKNLASFKVPKK
Sbjct: 425 DAVLLSHPDISQAVAFGVPDNKYGEEINCAIIPREGSSINEADVLEFCKKNLASFKVPKK 484

Query: 483 VFITDSLPKTATGKIQRRIVAEHFL 508
           VFITDSLPKTATGKIQRRIVAEHFL
Sbjct: 485 VFITDSLPKTATGKIQRRIVAEHFL 509

BLAST of MS017980 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 761.5 bits (1965), Expect = 4.2e-220
Identity = 389/507 (76.73%), Postives = 439/507 (86.59%), Query Frame = 0

Query: 3   TLSGLLKKAASEFPRRRALSVSGKFDLTHERLQELVEHAAS-LLADAGVNAGDVVALTFH 62
           TLSGLL+  A +FP RRALSVSGKF+LTH RL +L+E AAS L++DAG+  GDVVALTF 
Sbjct: 5   TLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVALTFP 64

Query: 63  NTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKL 122
           NTVEFVIMFLAVIR RATAAPLN AYTAEEFEFYLSDS+SKLLLTS+EG   AQ AA KL
Sbjct: 65  NTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEAASKL 124

Query: 123 NIPQVKASLSGDDEFLNLSPSGTESKSNMA-EIVNDPSDAALFLHTSGTTSRPKGVPLTQ 182
            I  V A+L      L LS + ++S  + A E+VN P D ALFLHTSGTTSRPKGVPLTQ
Sbjct: 125 KISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGVPLTQ 184

Query: 183 QNLAASVQNIKSVYKISESDSTVLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSAST 242
            NLA+SV+NIK+VYK++ESDSTV+VLPLFHVHGL+AGLLSSL +GAAVTLPAAGRFSA+T
Sbjct: 185 LNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRFSATT 244

Query: 243 FWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSF 302
           FWPDM  YNATWYTAVPTIHQIILDRH + PE  YPKLRFIRSCSASLAP I+ RLE +F
Sbjct: 245 FWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRLEEAF 304

Query: 303 GAPVLESYAMTEASHLMASNPLPEDGVHKAGSVGKPVGQEMAILDENGVPQSEGVSGEVC 362
           GAPVLE+YAMTEA+HLM+SNPLPE+G HK GSVGKPVGQEMAIL+E G  Q     GEVC
Sbjct: 305 GAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNKGEVC 364

Query: 363 IRGPNVTKGYKNNPEANKAAFLYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPI 422
           IRGPNVTKGYKNNPEANKA F +GWFHTGDIGY D+DGYLHLVGRIKELINRGGEKISPI
Sbjct: 365 IRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEKISPI 424

Query: 423 EVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVP 482
           EVD+VLL+H D++Q VAFGVPDEKYGEEINCA+IPREG+++ E ++  FCKKNLA+FKVP
Sbjct: 425 EVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAAFKVP 484

Query: 483 KKVFITDSLPKTATGKIQRRIVAEHFL 508
           K+VFITD+LPKTA+GKIQRRIVA+HFL
Sbjct: 485 KRVFITDNLPKTASGKIQRRIVAQHFL 511

BLAST of MS017980 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 201.8 bits (512), Expect = 1.3e-51
Identity = 161/520 (30.96%), Postives = 248/520 (47.69%), Query Frame = 0

Query: 2   STLSGLLKKAASEFPRRRALSVSGKFD-LTHERLQELVEHAASLLADAGVNAGDVVALTF 61
           ++L   L + +S +P + A++ S   D LT  +L+  V   A      G+   DVV +  
Sbjct: 27  TSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFA 86

Query: 62  HNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSESKLLLTSEEGIPSAQSAALK 121
            N+ +F + FLAV          NP YT  E    + DS  K++++  +     +     
Sbjct: 87  PNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKG---- 146

Query: 122 LNIPQVKASLSGDDEFLNLSPSGTESK-------SNMAEIVND-------PSDAALFLHT 181
            ++P V   L G  + + + P G+ SK         ++E V++        SD A  L++
Sbjct: 147 FDLPVV---LLGSKDTVEI-PPGSNSKILSFDNVMELSEPVSEYPFVEIKQSDTAALLYS 206

Query: 182 SGTTSRPKGVPLTQQN-LAASVQNIKSVYKISESDSTVL-VLPLFHVHGLMAGLLSSLIS 241
           SGTT   KGV LT  N +AAS+        + E     L  LP+FHV GL     S L  
Sbjct: 207 SGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQR 266

Query: 242 GAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQIILDRHLNKPEAAYPKLRFIRSC 301
           G A  L +  RF       ++  +  T    VP +   +  + + K +     L++I S 
Sbjct: 267 GNA--LVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVK-KFDLSSLKYIGSG 326

Query: 302 SASLAPSIMERL-ENSFGAPVLESYAMTEASHLMASNPLPEDGVHKAGSVG--KPVGQEM 361
           +A L   +ME    N     +++ Y MTE   +++    P  G   +GS G   P  +  
Sbjct: 327 AAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVED-PRLGKRNSGSAGMLAPGVEAQ 386

Query: 362 AILDENGVPQSEGVSGEVCIRGPNVTKGYKNNPEANKAAF-LYGWFHTGDIGYLDSDGYL 421
            +  E G  Q     GE+ +RGPN+ KGY NNP+A K       W HTGD+GY + DG L
Sbjct: 387 IVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNL 446

Query: 422 HLVGRIKELINRGGEKISPIEVDSVLLSHVDIAQAVAFGVPDEKYGEEINCAIIPREGSS 481
           ++V RIKELI   G +++P E++ +L+SH DI  AV    PDE+ GE     ++    SS
Sbjct: 447 YVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSS 506

Query: 482 IDEAEVMRFCKKNLASFKVPKKVFITDSLPKTATGKIQRR 501
           I E ++ +F  K +A +K  ++V     +PK+A GKI RR
Sbjct: 507 ITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRR 534

BLAST of MS017980 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 196.8 bits (499), Expect = 4.2e-50
Identity = 144/487 (29.57%), Postives = 238/487 (48.87%), Query Frame = 0

Query: 28  DLTHERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAY 87
           +LT   L   VE  A  L++ G+  G VV L   N++ F ++ L+V+   A     NP  
Sbjct: 57  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 116

Query: 88  TAEEFEFYLSDSESKLLLTSEEGIPSAQSAALKLNI---PQVKASLSGD----DEFLNLS 147
           T+ E    + DS   L  T+ + +P   +AA KL I    + +    GD     E +   
Sbjct: 117 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 176

Query: 148 PSGTESKSNMAEIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSVYKISESD 207
           PSG   K  +     D  D A  L++SGTT   KGV  + +NL A VQ I + +   + +
Sbjct: 177 PSGNRVKERV-----DQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGE 236

Query: 208 ST-VLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTI 267
              +  +P+FH++GL A     L  G+  T+    +F        +  Y AT    VP I
Sbjct: 237 QRFICTVPMFHIYGLAAFATGLLAYGS--TIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 296

Query: 268 HQIILDRHLNKPEAAY--PKLRFIRSCSASLAPSIMERLENSF-GAPVLESYAMTEASHL 327
             + +    ++ +A Y    +  +    A L+  + E     +    +L+ Y +TE++ +
Sbjct: 297 -LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGI 356

Query: 328 MASNPLPEDGVHKAGSVGK-PVGQEMAILDE-NGVPQSEGVSGEVCIRGPNVTKGYKNNP 387
            AS    E+   + G+ GK     E  I+D   G       +GE+ ++GP++ KGY +N 
Sbjct: 357 GASTDTVEES-RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNE 416

Query: 388 EANKAAF-LYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDIA 447
           EA  +     GW  TGD+ Y+D DG++ +V R+KELI   G +++P E++++LL+H +I 
Sbjct: 417 EATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEIT 476

Query: 448 QAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKKVFITDSLPKTA 501
            A     PD++ G+     ++ + GSS+ E  +M F  K +A +K  +KV    S+PK  
Sbjct: 477 DAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNP 534

BLAST of MS017980 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 194.9 bits (494), Expect = 1.6e-49
Identity = 151/488 (30.94%), Postives = 235/488 (48.16%), Query Frame = 0

Query: 30  THERLQELVEHAASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAYTA 89
           T+     +    AS L   G+  GDV+ +   N+ EFV  F+      A +   NP YT+
Sbjct: 77  TYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTS 136

Query: 90  EEFEFYLSDSESKLLLTSEEGIPSAQSAALKLNIPQVKASLSGDDEFLNLSPSGTESKSN 149
           +E    L  S +KL++T  + +   ++  L  N+  +       +  L  S   T+ ++N
Sbjct: 137 QELYKQLKSSGAKLIITHSQYVDKLKN--LGENLTLITTDEPTPENCLPFSTLITDDETN 196

Query: 150 MAEIVND--PSDAALFLHTSGTTSRPKGVPLTQQNLAASVQ-----NIKSVYKISESDST 209
             +   D    DAA    +SGTT  PKGV LT ++L  SV      +  ++Y +  +D  
Sbjct: 197 PFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLY-LKSNDVI 256

Query: 210 VLVLPLFHVHGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQI 269
           + VLPLFH++ L + LL+SL SGA V L    +F        +  +  T    VP +  I
Sbjct: 257 LCVLPLFHIYSLNSVLLNSLRSGATVLL--MHKFEIGALLDLIQRHRVTIAALVPPL-VI 316

Query: 270 ILDRHLNKPEAAYPKLRFIRSCSASLAPSIMERLENSFGAPVL-ESYAMTEASHLM---- 329
            L ++          +RF+ S +A L   + + L       +L + Y MTEA  ++    
Sbjct: 317 ALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSL 376

Query: 330 --ASNPLPEDGVHKAGSVGKPV-GQEMAILD-ENGVPQSEGVSGEVCIRGPNVTKGYKNN 389
             A  P+P     K+GS G  V   E+ ++  E  +       GE+CIRG  + K Y N+
Sbjct: 377 GFAKEPIPT----KSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLND 436

Query: 390 PEANKAAF-LYGWFHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDI 449
           PEA  A     GW HTGDIGY+D D  + +V R+KE+I   G ++ P E++S+L++H  I
Sbjct: 437 PEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSI 496

Query: 450 AQAVAFGVPDEKYGEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKKVFITDSLPKT 501
           A A      DE  GE     ++   G+ I E +V  +  K +  +K   KVF   S+PK+
Sbjct: 497 ADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKS 554

BLAST of MS017980 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 192.6 bits (488), Expect = 7.9e-49
Identity = 145/485 (29.90%), Postives = 240/485 (49.48%), Query Frame = 0

Query: 41  AASLLADAGVNAGDVVALTFHNTVEFVIMFLAVIRCRATAAPLNPAYTAEEFEFYLSDSE 100
           A  LL D G+  GDVV +   NT+   I+ L+V+   A     NP  TA E    ++DS 
Sbjct: 78  ADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLNTASEILRQIADSN 137

Query: 101 SKLLLTSEEGIPSAQSAALKLNIPQVKASL---------SGDDEFLNLSPSGTESKSNMA 160
            KL  T+ E  P   S+ + + + +V+ +L             E +   PSG   ++ + 
Sbjct: 138 PKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVGNLTEMMKKEPSGQAVRNQVH 197

Query: 161 EIVNDPSDAALFLHTSGTTSRPKGVPLTQQNLAASVQNIKSVYKISESDSTVLVLPLFHV 220
           +      D A+ L++SGTT R KGV  +  NL A V    +        + +  +PLFH 
Sbjct: 198 K-----DDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEPFEQPQQTFICTVPLFHT 257

Query: 221 HGLMAGLLSSLISGAAVTLPAAGRFSASTFWPDMLTYNATWYTAVPTIHQIIL---DRHL 280
            GL+  +L++L  G  V +    RF        +  Y AT    VP +   ++   D+ +
Sbjct: 258 FGLLNFVLATLALGTTVVI--LPRFDLGEMMAAVEKYRATTLILVPPVLVTMINKADQIM 317

Query: 281 NKPEAAYPKLRFIRSCSASLAPSIMERLENSF-GAPVLESYAMTEASHLMASNPLPEDGV 340
            K + ++  LR +R   A L+  + +     +    V + YA+TE++   AS    E+  
Sbjct: 318 KKYDVSF--LRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYALTESNGAGASIESVEES- 377

Query: 341 HKAGSVG-KPVGQEMAILDEN-GVPQSEGVSGEVCIRGPNVTKGYKNNPEANKAAFLYGW 400
            + G+VG    G E  I+D N G       +GE+ ++GP++ KGY  N E  +     GW
Sbjct: 378 RRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKGYFRNEE--EIITSEGW 437

Query: 401 FHTGDIGYLDSDGYLHLVGRIKELINRGGEKISPIEVDSVLLSHVDIAQAVAFGVPDEKY 460
             TGD+ Y+D+DG+L +V R+KELI   G ++ P E++++LL+H DI  A     PD++ 
Sbjct: 438 LKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPDKEA 497

Query: 461 GEEINCAIIPREGSSIDEAEVMRFCKKNLASFKVPKKVFITDSLPKTATGKIQRRIVAEH 511
           G+     +  +  S++ E +V+ F  K +A +K  +KV   DS+PKT +GK  R+ + + 
Sbjct: 498 GQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLRKDLIKF 550

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159678.14.6e-28599.41oxalate--CoA ligase-like [Momordica charantia][more]
KAG7036051.12.5e-25488.56Oxalate--CoA ligase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038874771.13.2e-25488.71oxalate--CoA ligase-like [Benincasa hispida][more]
KAG6606105.15.5e-25488.91Oxalate--CoA ligase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023534085.17.2e-25488.36oxalate--CoA ligase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
M4IQQ73.3e-23380.23Probable CoA ligase CCL9 OS=Humulus lupulus OX=3486 GN=CCL9 PE=2 SV=1[more]
Q9SMT76.0e-21976.73Oxalate--CoA ligase OS=Arabidopsis thaliana OX=3702 GN=AAE3 PE=1 SV=1[more]
O749769.8e-12146.96Oxalate--CoA ligase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
P381377.5e-10544.34Oxalate--CoA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=... [more]
Q0K8445.3e-7433.60Probable sulfoacetate--CoA ligase OS=Cupriavidus necator (strain ATCC 17699 / DS... [more]
Match NameE-valueIdentityDescription
A0A6J1DZI22.2e-28599.41oxalate--CoA ligase-like OS=Momordica charantia OX=3673 GN=LOC111026021 PE=4 SV=... [more]
A0A5A7THK15.9e-25488.51Oxalate--CoA ligase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A6J1H1B75.9e-25488.36oxalate--CoA ligase-like OS=Cucurbita moschata OX=3662 GN=LOC111459244 PE=4 SV=1[more]
A0A6J1K3G77.7e-25488.36oxalate--CoA ligase-like OS=Cucurbita maxima OX=3661 GN=LOC111490818 PE=4 SV=1[more]
A0A1S3AT787.7e-25488.51oxalate--CoA ligase-like OS=Cucumis melo OX=3656 GN=LOC103482735 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48990.14.2e-22076.73AMP-dependent synthetase and ligase family protein [more]
AT4G05160.11.3e-5130.96AMP-dependent synthetase and ligase family protein [more]
AT1G20510.14.2e-5029.57OPC-8:0 CoA ligase1 [more]
AT1G65060.11.6e-4930.944-coumarate:CoA ligase 3 [more]
AT5G38120.17.9e-4929.90AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..405
e-value: 1.3E-106
score: 358.6
NoneNo IPR availableGENE3D3.30.300.30coord: 406..507
e-value: 3.8E-28
score: 100.1
NoneNo IPR availablePANTHERPTHR43201:SF17OXALATE--COA LIGASE-LIKEcoord: 3..510
NoneNo IPR availablePANTHERPTHR43201ACYL-COA SYNTHETASEcoord: 3..510
NoneNo IPR availableCDDcd05926FACL_fum10p_likecoord: 16..504
e-value: 0.0
score: 741.051
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 3..508
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 9..412
e-value: 1.6E-96
score: 323.5
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 421..496
e-value: 5.9E-17
score: 62.4
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 163..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017980.1MS017980.1mRNA