MS017846 (gene) Bitter gourd (TR) v1

Overview
NameMS017846
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionValyl-tRNA synthetase
Locationscaffold373: 3164124 .. 3172918 (-)
RNA-Seq ExpressionMS017846
SyntenyMS017846
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGCAGGCAAGAGAAAAAGAATTGAAAAAGAAAAAGGCTTTAGAGAAAGCTTTGAAGCTGCAGGTAACCTTTTTTTTTGTTTTTTTTTTGTAAGTAACGTTTATGGTTTATCAATCATAGTATGTTTTACAGTGCAGTTTAAGATATCATTCCTTGGTTTTTCACCTTTTTTTACTCGGCAAGTCGGCATCATTTGTATAAATATACAAATTGATGGCTTAAGTTTGTAATTGATATTAAAATTTTCCAACTTATTTTAGGCTCAACAAACATCAAATGCTCCTAAAAAGAGTGAGAAGAAAAATGCCAGACGAGGTGGAGATGAAGAAAATGCTGAGGATTTTGTTGACCCTAACACCCCTTCTGGTGAAAAGAAACTGTTGTCTCAGCAAATGGCAAAGCAATACAATCCAAGTGCAGTGGAGAAGTCGTGAGTGTGGCATTTTGATTCCTATGACTTTTCATGCCTTGATTAAATTGACTTCATGATGCAAATCATAAAATTCTTGTTGCGGATCTAGGTGGTATGAGTGGTGGGAAAAATCAAGATTTTTTGTTGCTGATGCTAAAAGCTCCAAGCCGCCATTTGTAATTGTGAGCTTCTTTCATGACTTTGATGTTTGGTGCTTGAATTTCGGAATATAAGATCGTCCTTTTGCATGTTTTTCAGTTTTTCATATGATGTGGGATAGAACTCTTATAACTTAAGTGTTTATTTAAGATTTTCCTTCATTTTATGCGTTTTGCTTAGACCATATGATGTCTATCCTTCTTATTTTGGACCAAAAGATTATGGTTTAAATTTGCATTAGTAAATTCCACATTCACGATTGTGTTTTTAGTTCTCATATGTTTTTCAACAATACAGATTATTTTATTTTTTTATTTTTTTTGGAGAAGAGTCACCAACACAAAAATTGTAACATGCGCACCTACACTACGGCCACATTCTTCCAAGAAATTATGTGACTTTGACGTCCATATGTTAGCACATTTATATTAATTAGATTTAAATGTATTCATTGAATTGAAAACTTGTGTACTAAAAATAAATTTCATTGATCTGTCACTGTATCTTCGTATTTGGCAGGTGTTGCCCCCACCTAATGTGACAGGGGCCCTCCACATAGGTCATGCTCTTACTACTGCAATAGAGGTGCCGTAAATGCATTGACAGACAATTTCTTTATGCTGTTCATATCTACTGTAAGAAACTTCTTTTCTGGTCCAAGTGATTTTTTTGGGTCATGCAGGATGCTATCATTAGATGGCGGAGAATGTCTGGATATAACACCTTGTGGGTTCCTGGGACGGATCATGCTGGGATAGCAACCCAGGTTTTCACTTTTTATGTGTTAGTTTTCATTTCTTTTGATATTGATGGGATGGGGCATGCTGGGCAAGAGCATGAATGAGGTTTTTACCTTAATGGTTCAATGTTAAACCCCAATAACCAGAATATTATTGAAATATAAAAGATAGAAGAATACAATAACTCTTAAGTATTTCAAGGTACTTGGACCCCTCCATCTCTTGATTATTATTACTCAAGCCTACAATTGCTTGCCTTCATCTCCTCCTCCTCCCTTATGTATATCCAATTCCTTGGTTAATGACTAATATACCCTGACTACCTAATAATATTTCTAACTATTCAAGTGACATTCCTATTAGTTGTCCCTTTGTTGATTGGGCCTTGTTTATTGGGAGGCTCTTTTTTCTGTAGTTTATTGTTTTTAATGAAAGATGTTACCCTTGTAAAAACAAAATAAAAGAACAAAAATTTCTTAAATAAAGATTCTAAAGGTCATTCTTAGTTTCCATAAATTATTATCACTTATAGGATTGACTAATTCTTTAACTCGTCTTCAATGTTTGATTTAAAGCTTGATGGTAGAAGTAGCAATGGGCTCAATTACCATAATGGTGTACTTGCATTACAAAGTGAACTTTTATTTTTGAAGAATATATAGTTAACTGGTGTTATCTATTTCACAAGAGGCATAAGACTCGAGGCAAGGCAAAAAGTTTTTGTGCCTTGCTCTGAGGCTCGTAAGGGTTTTATAACGTTGCCCATGTGTGGCATATTAAAATAGGAATTATGCATTTGCTACCCACTATGCAAAATGTCACATCTAGGCATTTTTATTTGCATATGTTGATGAGTAAAATGTCATATTCCAGTGGCAATTGGTCTTCGCCATATCGTTGCTTAATTTTCATCTACTTATGCATATATGTAATATGATTGTCTAGAGTAAAGAGTTTCCCCTTTTCTTTTCGTGTAGGTTGTTGTAGAAAAGAAGATAATGCGGGAAAGGAATTTAACCAGGCATGATATTGGTCGGGAGAAGTTTATTTCTGAAGTAAGTTTGAACTCTACATCACTGATGTCAACTTTTCTTCTTTCTTTGGTGTTTTCATTTATTAAGCTGGCTTTCACTATCCATTTTACTTATGGTTGCATCAATTTTTGGGTACAGGTTTGGGATTGGAAAACCAAGTATGGTGGTACAATATTAAAACAGTTGCGCCGTTTGGGTGCCTCTCTTGATTGGTCCCGTGAGGTAATTGTTTAATGTATTCCTGCTTCAATTGCAGGTGATGTTACATTATTGACAGTACTAGCGCCATTATAGACCACAATACATATTTTGGATTTTTCTGTGACATTCTTTATTGCCTTTTAATTTTTAAATTTTGCTGCATACTCGTTTTAGTGCTTCACAATGGACGAGAAGAGATCAAAGGCCGTCACAGAGGCTTTTGTTAGGCTTTTCAAAAGTGGACTTATTTATAGGTATGCCTGAAGTATAAGTTATGTAACATTTGTTCATTTTGAATTTTGGAGGTGATTAGTGGCCTTGGAAAAATAACTACCAAATACTTACTGGTTAGGTTGGCAAGCTATGAAATTAGATGTCTTTGAAGTAATGAGTTCAAGTCTTTGTTGCACTTGGATATTTACATCCTATAAAGTTAATTGATGCCAAATTTTTTCAGGTTTAGATGGTTCATCAGTAGGATCAAGGTTATGGCCTGCATAAACTTGCTTTGATGTTAAGGATCATTGTAAAAGAAAAGAAAGAAAATCTTTGGAGGGGGTTTCCTCTTCCCCGGCCTTCAGATTGTTTTATTTTAGTTTTGTGAATATTCCGCCTCTTCTGTTAAAATGATAATAAATAGAGGGAAAATGGTGCCTTGTGGTCCGGCATGACTGTGGTCGACATGCTTACACAAACAGGTGTTTCAATATTACTCTAACTAAAAGCTGGCATGTACGATACTCAAAGATGTCTATATTCATAATAGAAAAAGCTTAACAGTTGAGTGTTATTGGAAAGTATCTAATTTTTGGATAGGATGAAGAAATTATGGTTGCTGATTAATGTTACAATATCAGTAATTTGACATTTGTTTATCATTTTCGTCCAGGGATTTACGTCTGGTGAACTGGGATTGTGTCTTGCGTACTGCAATATCTGATATTGAGGCAAGTTAATTACTATGAGGGGCGTGTTTTCTTAATCTATTATGTATTTATTAATGCTTCTGTCCATGTTTAGGTGGACTATATTGATATCAAAGAGAAGACTCTTCTCAAGGTTCCGGGATATGAGAACCCTGTGGAATTTGGTGTTCTGACCTCATTTGCTTATCCCCTCGAAGGGGAATTAGGCGAGATTGTGGTGGCCACTACCCGAGTAGAAACTATGCTTGGTGATACTGCAATTGCCATTCATCCTGATGACACGAGGTACAAGCATCTTCATGGAAAATATGCTATTCATCCTTTCAATGGAAGGAAGCTTCCTATAATTTGTGATGCAATTCTTGTTGATCCAAAATTTGGGACTGGTGCTGTTAAGGTAGGAGTTTCCTTTTTGCTTCTGAGGTACTAATGTTAGTTATACTTTTTTATTTTGCTTTCATGATTCAAGTGTTTCTCCACCTCCCCTCCCCCGTTTCTTATTTCTGCAGATCACACCTGCTCATGACCCGAACGATTTTGAGGTTGGAAAGCGTCATAATCTTGAGTTCATAAATATTTTTACTGATGATGGTAAAATAAATAGCAATGGTGGCTCAGAGTTTGCAGGATTGCCACGTTTTAAAGCACGAGAGACAGTAGTGGACGCCTTGCAGAAGAAGGTATTATACTAGAACTACTTATCTATTCTTAACTATTTTTTCTTCAAACTGCTATTAAATTGTGGGGCAAGCCCTAGCTGGGTTTGTTGACCCAGAATGCTAATGACTAGTAAACTTTGTTTTTATGGGAACATTAAGATAAAACTATCTTTTGTTGCTTTTAATTGAATTTTCCTCCCACAATTTATGCCAATTTTTCAACTTGCTTTCTAAACCTGCAAAGTGCATCTTTTGAAATGAAGGGACTGAAAGTAGTTGACACATTTGAGCAAGTTCACGTCTAATTTGTATGTCTCGTACCCATGAACTCGTAATCAGAGTTTGGCACATTTAATTTTGATAAAATTTGAGCATAAATTATCTCATTTGATGTAAAATTCTTTCTTGTTATTATATTTAATAATATGTTTTATGTGCTACTATCATAAGATTGTAGTGTTACGTAAAGCTCTGTAGAGAAATATACTGGTAATGATAGAAATACAGAAGGGTTACTACCAATTAATTTGAGGTCTTGGACCCCCTCCCTCTCTTGGGATTACTCAAGCCTGCACTAACTAAATCAATGTCGACCTTCATCTCATCCCACTCTCTCTAGTCTCTATTTATAACCAATAACTTGGCTAATAACTAATAAGGGCATTTTAGTTAAGCATGGAACTATAGAGCCGTCTCATGATGGTTCAAGTGGTCAATGAGGACCAATGTAAATAGCAAAGGACTTAGAGGGAATGTGTTCAAACCATGGTAGCCACCTACCTAGGATTTAATATCCTAGTCAACCAAATGTAGTAGGGTCAGGCGGTTGGCCCGTGACTAGTCGAGGTGCACGTAAGCTGGCCCGGACACACGGATATCAAAAAAAAAAAAAAGTTAGCATGGAACTACATGCTCCACTAACAAATTTATTTTCATCTACTTGATGCCAGTTTTGGCTCATTTGAGAGTGCAAAATTATATTCAAGTCATCTTTGTTGTATAATAGCTATTATATGTGACCATCTAAATTAATGATTGTTACGTTGCAATTTCCATGAGGTATCTTATATGTCTGTAGGGTCTTTATAGAGGTGCTAAAGACAATGAGATGCGTCTTGGCCTTTGTTCAAGGAGCAACGATGTTGTGGAGCCAATGATAAAGCCTCAATGGTATGTTAATTGCAAAGATATGGCAAAACAGAGTCTTGATGCTGCAACGGATAATGAATCCAGAAAGATTGAGATCATACCAAAACAATATTCTGCCGACTGGAAGAGGTTATCTTGCTTATTTGACTAAGTTGCATTGCATTTACACTTAAAATAAAATTGTGAATACGATTTGTTGGTATAAAATGTGAGGTTCTGAAATATTATTTTCAATGTTTTGGGTTCATCTTGGTGAATTCTCTATAAGTTCATTTACATAATAAATTACAATTATTTTTTACATATGCAAATAGAAGGAATTTTTTATGTATTCTTTTGGATATGAGTGGGATAATGTCTTATGTCCCCCGTTCAGTTGTACCTCTCTTTTATTTCAATAAGAGTTCATTTCCTAAAGTGTTATGGCTAAATTGTTGTTTCTTGTACTTTCTTAGTTTGGGCATGAGTTTTCATTGACCTCTACCTTAATTCCATCGCCATCCTTTCGCTCTGGCATGTGAAGCATTGTTCTAATATAGGTTGTATGCCACAGGTGGCTAGAGAACATTCGTGATTGGTGCATCTCAAGGCAACTTTGGTGGGGTCATAGGATTCCGGCATGGTACGCTACATTGGAGGATGATCAACTGAAGGAGTTTGGTGCTTACAACGATCATTGGGTGGTTGCAAGAAGTCAGGAAGAGGCTGAGGTGGAGGCTAAACAAATTTATGCAGGAAAAAGGTTCCATTTAGCACAAGATCCTGATGTGCTTGATACTTGGTTTTCCTCGGGTCTCTTTCCATTATCTGTGTTAGGTTGGCCTGATGATACTGAAGATCTAAAGACATTCTACCCAACATCAGCGCTAGAAACTGGTCATGACATTCTGTTTTTCTGGGTTGCTCGCATGGTAATGTTAGGAATCACGTTAGGTGGAGATGTACCTTTCCGAACGGTAATACAACTTCTCCCTATTTTTCACATGCTAGAAACTTTTTCCATTCCACCTCGTATCCACATCATGTGACAAATGCACTCTAAATTGATTATTTTAGATATATTTGCATCCGATGATTCGTGATGCGCATGGACGTAAGATGTCCAAGTCCTTGGGAAATGTCATTGATCCAGTTGAGGTGATAAATGGGATATCTCTTGAAGGCCTTCATAAAAGGCTGGAGGAGGGCAATCTGGACCCCAAAGAGTTGGCTACTGCCAAGGAAGGACAGGTTAAGGACTTCCCTAATGGTATTTCTGAATGTGGTGCAGATGCTCTCCGTTTTGCATTGATCTCCTACACTGCTCAGGTTTGTCTTTGGTTGTGAATTTATCTGCTGTTTCTAGAAATTGTGTAACTGATTACTGCTCTCTCTCTCTCTCCCCCTTGGTTCATCAGTCTGATAAAATAAATTTGGATATCCAAAGAGTTGTCGGTTACCGTCAATGGTGCAATAAATTATGGAATGCAATCCGATTTGCTATGAGTAAGCTCGGAAATGATTATGTTCCAAGCAATAATGTGACACCGGACACCTTACCATTTAGCTGTCAGTGGATACTGTCGGTACTAAACAAGGCTATATCCAGAACTGTTTCATCACTGGAAGCATACGAGTTCTCGGATGCAACCACTGCTGTCTATTCATGGTGGCAATACCAATTATGTGATGTCTTCATTGAAGCAATTAAGCCATACTTTTCCTCCAATGACACAGAATTTGCGTTGGCAAGAAGTCACGCACAAGATACATTATGGTTATGTCTTGACAATGGATTGCGATTGCTTCACCCGTTTATGCCTTATGTCACAGAAGAACTATGGCAGCGCCTTCCGTCCCCAAAGAGTAGTGCGAGGCTAGAATCGATTATGATATGCAACTATCCATCCGTGGTAGAGGTTAGTTTTTCCTTGAAGGTGCATGCATATGAATCTATGAGGTTCCATCATGGTATAGCTCCAAGGTGAATTTGAGGATAATGTTTAGTAAAACTGTACAGGATAGGTCCGGTTTATATTGTTAATGTAGTTATAAATACTGCACCTTCTAGTTCTTGATTATGACTCAACCCGGAGTTTCTTCCTGTCTTGAATGTTATTGTTATGTAATTGAAAGGTCAGGCTGATGACTTGGCCGATCATATGCATCTGTAATCATGAAATTGCTGATTAGGGTTCTTGAATTCTTCAGGAATGGACTAATGACGCTGTGGAGAATGAGATGGATCTTGTAGTGTCCGCTGTTAAATCTCTAAGGTCACTGGCAAAAGAAAGCCGTGAAAGGTTTGTTATCAATTTCACGATTGTTCTACTTGACGTTGCAAAAACAATTCAATCTATTGTTAACTTGAAGTCTACGAAGCTGGGTAGTGTTTCACTTCTTAAGAATCTACATTTTGTCATTTTTTCTTTTCATTAGCTGTTTAGTTAACCTGTGGAGATTTAAGGGAGTGATTTTGGTTTTGTCATTTTCTAAAATCATTTGTATATCATTGTCAAATTTTGACAATTATAAAAATGATTTTGACATGACCAAAATCATTTCCAAACGTGCCCTTACATTTGTCAATGAACTTCAAGCGATGTAAGAAAATGGCAAAAGTGATTTAGGCATCTCATTCATGCTTTTAAACTTTTTGGGAGCTGTTTGATCCAATTTATCGTGAGATGCCATGGTCCGGGAAGAAATAGATGTTAATGAGATGATTAACAATGGACATATGGTTCTGTAAACTGTTGGGGGTGGGGTCTGAGTTGGGTTTTTCTTTAAAATATAATTATGGACCTTTGACATTGGCCATGTGTAATATGATTTTCCCTCTTTTTATCTGAGCTGTTTTGTTTTAATTTAATTCAGGAGACCTGGCTACGTGCTCCCTCGAAATGATGCAGTTACCGAGACCGTCAACAAACGCAAGTTGGAAATAGTGACCCTTGCCAATTTATCATCTTTAACGGTATAGTTCCATTAATATGATAACAATAATAATAGTCTCTCTTGCAATTTCCTATTCTAGGGGTGGGGGATGAGTTCTCTTTCATGTTTACTTTGATCCAATCATCATCTCTATTAATCTCTATTCAGGTAATCAACGACCATGACGCCGCTCCGGTCGGGTGTGCAGTATCTGTTGTAAACGAAAACCTATCAGTCTATCTCCAGTTTCAAGGAGTGATTTCTGCTGAAGCCGAGCTCGAAAAGATCAGGAAAAAGATGGACGAGGTTAAAAAGTAAGCTGCCTGCTTGGTTCTAAGCAAAAATGGTTACATCTGCATCTCATCTCATTTTTTGGTACCCTGACATTTTATCTTATATCCATCATCATTTGTATACTTCGTAAACTTTTTCATTCGTGGTGCAGGCAACAGGAGAGGCTGACGAAGATGATGGACGCGAACGGATACAAAGAGAAGGTCCCGGCGCATATTCACGAGGAGAACATGAATAAGGTAGCATCCCTTATGCAGGAGCTTCTGTCTCTTGAAGAAGCAGGGCAGCATATTGAGGCTCACAAA

mRNA sequence

TGGCAGGCAAGAGAAAAAGAATTGAAAAAGAAAAAGGCTTTAGAGAAAGCTTTGAAGCTGCAGGCTCAACAAACATCAAATGCTCCTAAAAAGAGTGAGAAGAAAAATGCCAGACGAGGTGGAGATGAAGAAAATGCTGAGGATTTTGTTGACCCTAACACCCCTTCTGGTGAAAAGAAACTGTTGTCTCAGCAAATGGCAAAGCAATACAATCCAAGTGCAGTGGAGAAGTCGTGGTATGAGTGGTGGGAAAAATCAAGATTTTTTGTTGCTGATGCTAAAAGCTCCAAGCCGCCATTTGTAATTGTGTTGCCCCCACCTAATGTGACAGGGGCCCTCCACATAGGTCATGCTCTTACTACTGCAATAGAGGATGCTATCATTAGATGGCGGAGAATGTCTGGATATAACACCTTGTGGGTTCCTGGGACGGATCATGCTGGGATAGCAACCCAGGTTGTTGTAGAAAAGAAGATAATGCGGGAAAGGAATTTAACCAGGCATGATATTGGTCGGGAGAAGTTTATTTCTGAAGTTTGGGATTGGAAAACCAAGTATGGTGGTACAATATTAAAACAGTTGCGCCGTTTGGGTGCCTCTCTTGATTGGTCCCGTGAGTGCTTCACAATGGACGAGAAGAGATCAAAGGCCGTCACAGAGGCTTTTGTTAGGCTTTTCAAAAGTGGACTTATTTATAGGGATTTACGTCTGGTGAACTGGGATTGTGTCTTGCGTACTGCAATATCTGATATTGAGGTGGACTATATTGATATCAAAGAGAAGACTCTTCTCAAGGTTCCGGGATATGAGAACCCTGTGGAATTTGGTGTTCTGACCTCATTTGCTTATCCCCTCGAAGGGGAATTAGGCGAGATTGTGGTGGCCACTACCCGAGTAGAAACTATGCTTGGTGATACTGCAATTGCCATTCATCCTGATGACACGAGGTACAAGCATCTTCATGGAAAATATGCTATTCATCCTTTCAATGGAAGGAAGCTTCCTATAATTTGTGATGCAATTCTTGTTGATCCAAAATTTGGGACTGGTGCTGTTAAGATCACACCTGCTCATGACCCGAACGATTTTGAGGTTGGAAAGCGTCATAATCTTGAGTTCATAAATATTTTTACTGATGATGGTAAAATAAATAGCAATGGTGGCTCAGAGTTTGCAGGATTGCCACGTTTTAAAGCACGAGAGACAGTAGTGGACGCCTTGCAGAAGAAGGGTCTTTATAGAGGTGCTAAAGACAATGAGATGCGTCTTGGCCTTTGTTCAAGGAGCAACGATGTTGTGGAGCCAATGATAAAGCCTCAATGGTATGTTAATTGCAAAGATATGGCAAAACAGAGTCTTGATGCTGCAACGGATAATGAATCCAGAAAGATTGAGATCATACCAAAACAATATTCTGCCGACTGGAAGAGGTGGCTAGAGAACATTCGTGATTGGTGCATCTCAAGGCAACTTTGGTGGGGTCATAGGATTCCGGCATGGTACGCTACATTGGAGGATGATCAACTGAAGGAGTTTGGTGCTTACAACGATCATTGGGTGGTTGCAAGAAGTCAGGAAGAGGCTGAGGTGGAGGCTAAACAAATTTATGCAGGAAAAAGGTTCCATTTAGCACAAGATCCTGATGTGCTTGATACTTGGTTTTCCTCGGGTCTCTTTCCATTATCTGTGTTAGGTTGGCCTGATGATACTGAAGATCTAAAGACATTCTACCCAACATCAGCGCTAGAAACTGGTCATGACATTCTGTTTTTCTGGGTTGCTCGCATGGTAATGTTAGGAATCACGTTAGGTGGAGATGTACCTTTCCGAACGATATATTTGCATCCGATGATTCGTGATGCGCATGGACGTAAGATGTCCAAGTCCTTGGGAAATGTCATTGATCCAGTTGAGGTGATAAATGGGATATCTCTTGAAGGCCTTCATAAAAGGCTGGAGGAGGGCAATCTGGACCCCAAAGAGTTGGCTACTGCCAAGGAAGGACAGGTTAAGGACTTCCCTAATGGTATTTCTGAATGTGGTGCAGATGCTCTCCGTTTTGCATTGATCTCCTACACTGCTCAGTCTGATAAAATAAATTTGGATATCCAAAGAGTTGTCGGTTACCGTCAATGGTGCAATAAATTATGGAATGCAATCCGATTTGCTATGAGTAAGCTCGGAAATGATTATGTTCCAAGCAATAATGTGACACCGGACACCTTACCATTTAGCTGTCAGTGGATACTGTCGGTACTAAACAAGGCTATATCCAGAACTGTTTCATCACTGGAAGCATACGAGTTCTCGGATGCAACCACTGCTGTCTATTCATGGTGGCAATACCAATTATGTGATGTCTTCATTGAAGCAATTAAGCCATACTTTTCCTCCAATGACACAGAATTTGCGTTGGCAAGAAGTCACGCACAAGATACATTATGGTTATGTCTTGACAATGGATTGCGATTGCTTCACCCGTTTATGCCTTATGTCACAGAAGAACTATGGCAGCGCCTTCCGTCCCCAAAGAGTAGTGCGAGGCTAGAATCGATTATGATATGCAACTATCCATCCGTGGTAGAGGAATGGACTAATGACGCTGTGGAGAATGAGATGGATCTTGTAGTGTCCGCTGTTAAATCTCTAAGGTCACTGGCAAAAGAAAGCCGTGAAAGGAGACCTGGCTACGTGCTCCCTCGAAATGATGCAGTTACCGAGACCGTCAACAAACGCAAGTTGGAAATAGTGACCCTTGCCAATTTATCATCTTTAACGGTAATCAACGACCATGACGCCGCTCCGGTCGGGTGTGCAGTATCTGTTGTAAACGAAAACCTATCAGTCTATCTCCAGTTTCAAGGAGTGATTTCTGCTGAAGCCGAGCTCGAAAAGATCAGGAAAAAGATGGACGAGGTTAAAAAGCAACAGGAGAGGCTGACGAAGATGATGGACGCGAACGGATACAAAGAGAAGGTCCCGGCGCATATTCACGAGGAGAACATGAATAAGGTAGCATCCCTTATGCAGGAGCTTCTGTCTCTTGAAGAAGCAGGGCAGCATATTGAGGCTCACAAA

Coding sequence (CDS)

TGGCAGGCAAGAGAAAAAGAATTGAAAAAGAAAAAGGCTTTAGAGAAAGCTTTGAAGCTGCAGGCTCAACAAACATCAAATGCTCCTAAAAAGAGTGAGAAGAAAAATGCCAGACGAGGTGGAGATGAAGAAAATGCTGAGGATTTTGTTGACCCTAACACCCCTTCTGGTGAAAAGAAACTGTTGTCTCAGCAAATGGCAAAGCAATACAATCCAAGTGCAGTGGAGAAGTCGTGGTATGAGTGGTGGGAAAAATCAAGATTTTTTGTTGCTGATGCTAAAAGCTCCAAGCCGCCATTTGTAATTGTGTTGCCCCCACCTAATGTGACAGGGGCCCTCCACATAGGTCATGCTCTTACTACTGCAATAGAGGATGCTATCATTAGATGGCGGAGAATGTCTGGATATAACACCTTGTGGGTTCCTGGGACGGATCATGCTGGGATAGCAACCCAGGTTGTTGTAGAAAAGAAGATAATGCGGGAAAGGAATTTAACCAGGCATGATATTGGTCGGGAGAAGTTTATTTCTGAAGTTTGGGATTGGAAAACCAAGTATGGTGGTACAATATTAAAACAGTTGCGCCGTTTGGGTGCCTCTCTTGATTGGTCCCGTGAGTGCTTCACAATGGACGAGAAGAGATCAAAGGCCGTCACAGAGGCTTTTGTTAGGCTTTTCAAAAGTGGACTTATTTATAGGGATTTACGTCTGGTGAACTGGGATTGTGTCTTGCGTACTGCAATATCTGATATTGAGGTGGACTATATTGATATCAAAGAGAAGACTCTTCTCAAGGTTCCGGGATATGAGAACCCTGTGGAATTTGGTGTTCTGACCTCATTTGCTTATCCCCTCGAAGGGGAATTAGGCGAGATTGTGGTGGCCACTACCCGAGTAGAAACTATGCTTGGTGATACTGCAATTGCCATTCATCCTGATGACACGAGGTACAAGCATCTTCATGGAAAATATGCTATTCATCCTTTCAATGGAAGGAAGCTTCCTATAATTTGTGATGCAATTCTTGTTGATCCAAAATTTGGGACTGGTGCTGTTAAGATCACACCTGCTCATGACCCGAACGATTTTGAGGTTGGAAAGCGTCATAATCTTGAGTTCATAAATATTTTTACTGATGATGGTAAAATAAATAGCAATGGTGGCTCAGAGTTTGCAGGATTGCCACGTTTTAAAGCACGAGAGACAGTAGTGGACGCCTTGCAGAAGAAGGGTCTTTATAGAGGTGCTAAAGACAATGAGATGCGTCTTGGCCTTTGTTCAAGGAGCAACGATGTTGTGGAGCCAATGATAAAGCCTCAATGGTATGTTAATTGCAAAGATATGGCAAAACAGAGTCTTGATGCTGCAACGGATAATGAATCCAGAAAGATTGAGATCATACCAAAACAATATTCTGCCGACTGGAAGAGGTGGCTAGAGAACATTCGTGATTGGTGCATCTCAAGGCAACTTTGGTGGGGTCATAGGATTCCGGCATGGTACGCTACATTGGAGGATGATCAACTGAAGGAGTTTGGTGCTTACAACGATCATTGGGTGGTTGCAAGAAGTCAGGAAGAGGCTGAGGTGGAGGCTAAACAAATTTATGCAGGAAAAAGGTTCCATTTAGCACAAGATCCTGATGTGCTTGATACTTGGTTTTCCTCGGGTCTCTTTCCATTATCTGTGTTAGGTTGGCCTGATGATACTGAAGATCTAAAGACATTCTACCCAACATCAGCGCTAGAAACTGGTCATGACATTCTGTTTTTCTGGGTTGCTCGCATGGTAATGTTAGGAATCACGTTAGGTGGAGATGTACCTTTCCGAACGATATATTTGCATCCGATGATTCGTGATGCGCATGGACGTAAGATGTCCAAGTCCTTGGGAAATGTCATTGATCCAGTTGAGGTGATAAATGGGATATCTCTTGAAGGCCTTCATAAAAGGCTGGAGGAGGGCAATCTGGACCCCAAAGAGTTGGCTACTGCCAAGGAAGGACAGGTTAAGGACTTCCCTAATGGTATTTCTGAATGTGGTGCAGATGCTCTCCGTTTTGCATTGATCTCCTACACTGCTCAGTCTGATAAAATAAATTTGGATATCCAAAGAGTTGTCGGTTACCGTCAATGGTGCAATAAATTATGGAATGCAATCCGATTTGCTATGAGTAAGCTCGGAAATGATTATGTTCCAAGCAATAATGTGACACCGGACACCTTACCATTTAGCTGTCAGTGGATACTGTCGGTACTAAACAAGGCTATATCCAGAACTGTTTCATCACTGGAAGCATACGAGTTCTCGGATGCAACCACTGCTGTCTATTCATGGTGGCAATACCAATTATGTGATGTCTTCATTGAAGCAATTAAGCCATACTTTTCCTCCAATGACACAGAATTTGCGTTGGCAAGAAGTCACGCACAAGATACATTATGGTTATGTCTTGACAATGGATTGCGATTGCTTCACCCGTTTATGCCTTATGTCACAGAAGAACTATGGCAGCGCCTTCCGTCCCCAAAGAGTAGTGCGAGGCTAGAATCGATTATGATATGCAACTATCCATCCGTGGTAGAGGAATGGACTAATGACGCTGTGGAGAATGAGATGGATCTTGTAGTGTCCGCTGTTAAATCTCTAAGGTCACTGGCAAAAGAAAGCCGTGAAAGGAGACCTGGCTACGTGCTCCCTCGAAATGATGCAGTTACCGAGACCGTCAACAAACGCAAGTTGGAAATAGTGACCCTTGCCAATTTATCATCTTTAACGGTAATCAACGACCATGACGCCGCTCCGGTCGGGTGTGCAGTATCTGTTGTAAACGAAAACCTATCAGTCTATCTCCAGTTTCAAGGAGTGATTTCTGCTGAAGCCGAGCTCGAAAAGATCAGGAAAAAGATGGACGAGGTTAAAAAGCAACAGGAGAGGCTGACGAAGATGATGGACGCGAACGGATACAAAGAGAAGGTCCCGGCGCATATTCACGAGGAGAACATGAATAAGGTAGCATCCCTTATGCAGGAGCTTCTGTCTCTTGAAGAAGCAGGGCAGCATATTGAGGCTCACAAA

Protein sequence

WQAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKLLSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLPSPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPRNDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQHIEAHK
Homology
BLAST of MS017846 vs. NCBI nr
Match: XP_022156305.1 (valine--tRNA ligase, mitochondrial 1 [Momordica charantia])

HSP 1 Score: 2075.8 bits (5377), Expect = 0.0e+00
Identity = 1022/1025 (99.71%), Postives = 1023/1025 (99.80%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL
Sbjct: 21   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 80

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT
Sbjct: 81   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 140

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 141  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 200

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFK GLIYRDLRLVNWD
Sbjct: 201  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKRGLIYRDLRLVNWD 260

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKE TLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 261  CVLRTAISDIEVDYIDIKENTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 320

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN
Sbjct: 321  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 380

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM
Sbjct: 381  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 440

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN
Sbjct: 441  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 500

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF
Sbjct: 501  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 560

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI
Sbjct: 561  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 620

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 621  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 680

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 681  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 740

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ
Sbjct: 741  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 800

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP
Sbjct: 801  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 860

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
            SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR
Sbjct: 861  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 920

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA
Sbjct: 921  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 980

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH
Sbjct: 981  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1040

Query: 1022 IEAHK 1027
            IEAHK
Sbjct: 1041 IEAHK 1045

BLAST of MS017846 vs. NCBI nr
Match: XP_038875929.1 (valine--tRNA ligase, mitochondrial 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1977.2 bits (5121), Expect = 0.0e+00
Identity = 960/1025 (93.66%), Postives = 996/1025 (97.17%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+T  G+KKL
Sbjct: 21   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTSFGKKKL 80

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            LSQQMAKQYNPSAVEKSWYEWWEKS FFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 81   LSQQMAKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 140

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKF+SEVWD
Sbjct: 141  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFMSEVWD 200

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 201  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 260

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 261  CVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 320

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGK+AIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN
Sbjct: 321  MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 380

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEFAG+PRFKARE VVDALQKKGLYRGAKDNEM
Sbjct: 381  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREVVVDALQKKGLYRGAKDNEM 440

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKD AKQSLDAA DNE +K+EIIPKQYSADWKRWLEN
Sbjct: 441  RLGLCSRSNDVVEPMIKPQWYVNCKDPAKQSLDAAMDNECKKLEIIPKQYSADWKRWLEN 500

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVAR++EEAE +A+QIYAGK+F
Sbjct: 501  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARTEEEAEEDARQIYAGKKF 560

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI
Sbjct: 561  HLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 620

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
             LGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVING+SLEGLHKRLEEGNLDPKE
Sbjct: 621  VLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGVSLEGLHKRLEEGNLDPKE 680

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 681  LAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 740

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVP+ NVTPD LPFSCQWILSVLNKAISRT+SSLE+YEFSDATTAVYSWWQ
Sbjct: 741  FAMSKLGNDYVPNKNVTPDVLPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWWQ 800

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSND EFA  RSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 801  YQLCDVFIEAIKPYFSSNDAEFASVRSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 860

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PKSS R ESIMIC+YP V+EEWTN+AVENEMDL+VSAVKSLRSLAKESRERRPGYV PR
Sbjct: 861  YPKSSTRPESIMICDYPLVIEEWTNEAVENEMDLIVSAVKSLRSLAKESRERRPGYVFPR 920

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            NDAV ET+NKRKLEIVTLANLSSLTVIND+DAAPVGC +SVVNENLSVYLQFQG ISAEA
Sbjct: 921  NDAVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCTISVVNENLSVYLQFQGAISAEA 980

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI+KKMDE+KKQQE+L KMMDANGYKEKV  HIHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 981  ELEKIKKKMDEIKKQQEKLKKMMDANGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1040

Query: 1022 IEAHK 1027
            I+A +
Sbjct: 1041 IQAQR 1045

BLAST of MS017846 vs. NCBI nr
Match: XP_023533939.1 (valine--tRNA ligase, mitochondrial 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1976.8 bits (5120), Expect = 0.0e+00
Identity = 963/1025 (93.95%), Postives = 995/1025 (97.07%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G KKL
Sbjct: 82   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKL 141

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            +SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 142  MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 201

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 202  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 261

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 262  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 321

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 322  CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 381

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGK+AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDPN
Sbjct: 382  MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 441

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEF G+PRFK RE VVDALQKKGLYRGAKDNEM
Sbjct: 442  DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEM 501

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDA TDNE +KIEIIPKQYSADWKRWLEN
Sbjct: 502  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNECKKIEIIPKQYSADWKRWLEN 561

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVVAR++EEAE EAK IYAGK+F
Sbjct: 562  IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 621

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPD+TEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 622  HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 681

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 682  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 741

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 742  LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 801

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPS NVTPD LPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ
Sbjct: 802  FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 861

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTE+A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 862  YQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 921

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PKSS R ESIMIC+YPSV EEWTN++VE EMDL+VSAVKSLRSLAKESRERRPGYVLPR
Sbjct: 922  YPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPR 981

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            ND V ET+NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSVVNENLSVYLQFQG ISAEA
Sbjct: 982  NDIVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 1041

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI+KKM+EV+KQQE+L KMMDA+GYKEKV  HIHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 1042 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1101

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1102 IQAHR 1106

BLAST of MS017846 vs. NCBI nr
Match: XP_022957954.1 (valine--tRNA ligase, mitochondrial 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1975.7 bits (5117), Expect = 0.0e+00
Identity = 961/1025 (93.76%), Postives = 996/1025 (97.17%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G+KKL
Sbjct: 82   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 141

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            +SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 142  MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 201

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 202  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 261

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 262  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 321

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 322  CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 381

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGK+AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDPN
Sbjct: 382  MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 441

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEF G+PRF+ RE VVDALQKKGLYRGAKDNEM
Sbjct: 442  DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFEVREMVVDALQKKGLYRGAKDNEM 501

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDA TDNE +KIEIIPKQYSADWKRWLEN
Sbjct: 502  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNECKKIEIIPKQYSADWKRWLEN 561

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVVAR++EEAE EAK IYAGK+F
Sbjct: 562  IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 621

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPD+TEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 622  HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 681

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 682  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 741

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 742  LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 801

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPS NVTPD LPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ
Sbjct: 802  FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 861

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTE+A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 862  YQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 921

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PKSS R ESIMIC+YPSV EEWTN++VE EMDL+VSAVKSLRSLAKESRERRPGYVLPR
Sbjct: 922  YPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPR 981

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            ND V ET+NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSV+NENLSVYLQFQG ISAEA
Sbjct: 982  NDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVINENLSVYLQFQGAISAEA 1041

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI+KKM+EV+KQQE+L KMMDA+GYKEKV  HIHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 1042 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1101

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1102 IQAHR 1106

BLAST of MS017846 vs. NCBI nr
Match: KAG6605705.1 (Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1974.5 bits (5114), Expect = 0.0e+00
Identity = 961/1025 (93.76%), Postives = 994/1025 (96.98%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G KKL
Sbjct: 64   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGNKKL 123

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            +SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 124  MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 183

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 184  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 243

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 244  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 303

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 304  CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 363

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGK+AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDPN
Sbjct: 364  MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 423

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEF G+PRFK RE VVDALQKKGLYRGAKDNEM
Sbjct: 424  DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEM 483

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDA  DNE +KIEIIPKQYSADWKRWLEN
Sbjct: 484  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVMDNECKKIEIIPKQYSADWKRWLEN 543

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVVAR++EEAE EAK IYAGK+F
Sbjct: 544  IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 603

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPD+TEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 604  HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 663

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 664  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 723

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 724  LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 783

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPS NVTPD LPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ
Sbjct: 784  FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 843

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTE+A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 844  YQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 903

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PKSS R ESIMIC+YPSV EEWTN++VE EMDL+VSAVKSLRSLAKESRERRPGYVLPR
Sbjct: 904  YPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPR 963

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            ND + ET+NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSVVNENLSVYLQFQG ISAEA
Sbjct: 964  NDVIAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 1023

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI+KKM+EV+KQQE+L KMMDA+GYKEKV  HIHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 1024 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1083

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1084 IQAHR 1088

BLAST of MS017846 vs. ExPASy Swiss-Prot
Match: P93736 (Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=1 SV=2)

HSP 1 Score: 1597.8 bits (4136), Expect = 0.0e+00
Identity = 758/1034 (73.31%), Postives = 880/1034 (85.11%), Query Frame = 0

Query: 2    QAREKELKKKKALEK----ALKL-QAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPS 61
            +A+EKELKK+KALEK     LK  QA+  +N PKKS KK+++R   EEN EDFVDP TP 
Sbjct: 67   KAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKKSSKRDASEENPEDFVDPETPL 126

Query: 62   GEKKLLSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIG 121
            GE+K LS QMAKQY+P+ VEKSWY WWEKS  F ADAKSSKPPFVIVLPPPNVTGALHIG
Sbjct: 127  GERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIG 186

Query: 122  HALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 181
            HALT+AIED IIRW+RMSGYN LWVPG DHAGIATQVVVEKKIMR+R +TRHD+GRE+F+
Sbjct: 187  HALTSAIEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMTRHDVGREEFV 246

Query: 182  SEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLR 241
             EVW WK +YGGTIL QLRRLGASLDWSRECFTMDE+RSKAVTEAFVRL+K GLIYRD+R
Sbjct: 247  KEVWKWKNQYGGTILTQLRRLGASLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIR 306

Query: 242  LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVAT 301
            LVNWDC+LRTAISD+EV+YIDIKEKTLLKVPGYE PVEFG+LTSFAYPLEG LGE++VAT
Sbjct: 307  LVNWDCILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFGLLTSFAYPLEGGLGEVIVAT 366

Query: 302  TRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITP 361
            TRVETMLGDTAIAIHPDD RYKHLHGK+A+HPFNGRKLPIICD ILVDP FGTG VKITP
Sbjct: 367  TRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFNGRKLPIICDGILVDPNFGTGCVKITP 426

Query: 362  AHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGA 421
            AHDPND EVGKRH LEFINIFTDDGKIN+NGGS+FAG+PRF ARE VV+ALQK+GLYRGA
Sbjct: 427  AHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPRFAAREAVVEALQKQGLYRGA 486

Query: 422  KDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWK 481
            K+NEMRLGLCSR+NDV+EPMIKPQWYVNC  + K++LD A  +E++K+E +PKQY+A+W+
Sbjct: 487  KNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVAITDENKKLEFVPKQYTAEWR 546

Query: 482  RWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIY 541
            RWLENIRDWCISRQLWWGHRIPAWYATLE+DQLKE GAY+DHWVVAR++++A  EA Q +
Sbjct: 547  RWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYSDHWVVARTEDDAREEAAQKF 606

Query: 542  AGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARM 601
             GK+F L +DPDVLDTWFSSGLFPLSVLGWPD T+D K FYPTS LETGHDILFFWVARM
Sbjct: 607  LGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVTDDFKAFYPTSVLETGHDILFFWVARM 666

Query: 602  VMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGN 661
            VM+G+ LGG+VPF  +Y HPMIRDAHGRKMSKSLGNVIDP+EVING++LEGLHKRLEEGN
Sbjct: 667  VMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDPLEVINGVTLEGLHKRLEEGN 726

Query: 662  LDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKL 721
            LDPKE+  AKEGQVKDFPNGI ECG DALRFAL+SYTAQSDKINLDI RVVGYRQWCNKL
Sbjct: 727  LDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQSDKINLDILRVVGYRQWCNKL 786

Query: 722  WNAIRFAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAV 781
            WNA+RFAM KLG+ Y P   ++P+T+PFSCQWILSVLNKAIS+TV SL+A+EFSDA   +
Sbjct: 787  WNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKAISKTVVSLDAFEFSDAANTI 846

Query: 782  YSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEEL 841
            Y+WWQYQ CDV+IEAIKPYF+ ++  FA  R+HAQ  LW+ L+ GLRLLHPFMP+VTEEL
Sbjct: 847  YAWWQYQFCDVYIEAIKPYFAGDNPTFASERAHAQHALWISLETGLRLLHPFMPFVTEEL 906

Query: 842  WQRLPSPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRS-----LAKESR 901
            WQRLP+PK + R  SIMIC+YPS +E W+N+ VE+EMD V++ VK +R+     L K+  
Sbjct: 907  WQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTVLATVKCMRALRAGLLEKQKN 966

Query: 902  ERRPGYVLPRNDAVTETVNKRKLEIVTLANLSSLTVIN-DHDAAPVGCAVSVVNENLSVY 961
            ER P + L  N+  +E V   +LEI TLANLSSL V++    AAP G +V  VNENL VY
Sbjct: 967  ERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKGQHAAPPGSSVETVNENLKVY 1026

Query: 962  LQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQ 1021
            L+  G I+ EAE EKIR K+ E++KQ+E+L KMM  + Y+EKVPA+I E+N NK+A ++Q
Sbjct: 1027 LEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYEEKVPANIKEDNANKLAKILQ 1086

Query: 1022 ELLSLEEAGQHIEA 1025
            E    E+    + A
Sbjct: 1087 EFDFFEKESARLAA 1100

BLAST of MS017846 vs. ExPASy Swiss-Prot
Match: Q86KU2 (Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=valS1 PE=3 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 6.6e-302
Identity = 524/1029 (50.92%), Postives = 693/1029 (67.35%), Query Frame = 0

Query: 4    REKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP--NTPSGEKKL 63
            +EKE K+  A EK L+ + ++     +K  ++  ++  +E+   + ++    TP GEKK 
Sbjct: 40   KEKEAKQAAAKEKELQNKLKKEQEKKEKEAEEKEKQEAEEKIVREHIEKILKTPKGEKKD 99

Query: 64   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPP-------FVIVLPPPNVTGALH 123
            +S  +   Y+P+AVE  WY++W  + +F  + +    P       FVIV+PPPNVTG+LH
Sbjct: 100  VS-SLLSSYHPTAVESIWYDYWLDNGYFSPEKQMEIQPHVVKDKKFVIVIPPPNVTGSLH 159

Query: 124  IGHALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREK 183
            +GHALT +I+DA++R+ RM G   LWVPGTDHAGIATQVVVEKKI +E  +TRHD+GRE 
Sbjct: 160  LGHALTNSIQDAVVRYHRMKGEVCLWVPGTDHAGIATQVVVEKKIWKENKITRHDLGREG 219

Query: 184  FISEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRD 243
            FI +VW+WK +YG  I  QL+++G+S+DWSRE FTMD++RSKAV  AF+R+F  GLI R 
Sbjct: 220  FIKKVWEWKGEYGARIQGQLKKMGSSVDWSREVFTMDDQRSKAVNTAFIRMFNDGLIVRT 279

Query: 244  LRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVV 303
             RLVNW C L+TAISDIEVD+ID+++ T + VPG++   +FGVL  FAY +EG   E+VV
Sbjct: 280  TRLVNWSCALKTAISDIEVDFIDLEKHTKMSVPGHQGQYDFGVLFEFAYQVEGSGEELVV 339

Query: 304  ATTRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKI 363
            ATTR+ETML DTAIAIHPDDTRY HLHGK+AIHP NGRK+PII D++LVD  FGTG VKI
Sbjct: 340  ATTRIETMLADTAIAIHPDDTRYTHLHGKFAIHPLNGRKIPIITDSVLVDKDFGTGVVKI 399

Query: 364  TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYR 423
            TP+HDPND+E   RH LEFIN+FTD+G IN NGG  FAG+ RF AR  VV+AL++K L++
Sbjct: 400  TPSHDPNDYETALRHGLEFINLFTDEGLINENGGERFAGMKRFDARNAVVEALKEKNLFK 459

Query: 424  GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSAD 483
            G KDN+MRLGLCSRS DV+EPMIKPQWYV C DMA +++ A  D +   ++I PK +   
Sbjct: 460  GMKDNKMRLGLCSRSKDVIEPMIKPQWYVKCDDMAARAVKAVRDGD---LKITPKTHEVT 519

Query: 484  WKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEA-- 543
            W RWLE I+DWC+SRQLWWGHRIPA++  ++  +   +    + WVV  +QEEA   A  
Sbjct: 520  WYRWLEGIKDWCVSRQLWWGHRIPAYHCIIKGVKSNPYD--TNQWVVGANQEEATQNAIE 579

Query: 544  --KQIYAGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILF 603
              K         L QDPDVLDTWFSSGLFP SV+GWP+ T+D++ +YPTS LETG DILF
Sbjct: 580  KFKLSSKDDILSLEQDPDVLDTWFSSGLFPFSVMGWPEQTQDMQDYYPTSLLETGSDILF 639

Query: 604  FWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHK 663
            FWVARMVM+G  L G +PF  ++LH M+RD+HGRKMSKSLGNVIDP +VI GISL+ L  
Sbjct: 640  FWVARMVMMGQQLTGKLPFEQVFLHAMVRDSHGRKMSKSLGNVIDPNDVIKGISLDELIA 699

Query: 664  RLEEGNLDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYR 723
            +L EGNLD KE+  A  G   DFP GI+ECG DA+RFAL +YT+Q   INLDIQRVVGYR
Sbjct: 700  KLYEGNLDSKEIEKATSGVKSDFPTGIAECGTDAMRFALCAYTSQGRDINLDIQRVVGYR 759

Query: 724  QWCNKLWNAIRFAMSKLGNDYVPSNNVTPDTLPFSCQ---WILSVLNKAISRTVSSLEAY 783
             +CNK+WNA RFA  KLG+ + P +      L  S     WIL+   +AI+ +    + Y
Sbjct: 760  NFCNKIWNATRFAHMKLGDSFKPESFDASKLLQQSNAINLWILNAAQRAITLSEEGFKEY 819

Query: 784  EFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHP 843
            +FS  TTA+YS+W  ++CDV++E  K  FS    E +  +   ++TL+ C+D GLRLLHP
Sbjct: 820  DFSKVTTAIYSFWLNEMCDVYLEMTKSIFSLE--EDSPIKQKTKETLYTCIDIGLRLLHP 879

Query: 844  FMPYVTEELWQRLPSPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLA 903
            FMPY+TEEL+Q LP       + +IM+C YP     W N  +E EM      +KS+RSL 
Sbjct: 880  FMPYLTEELYQSLPRRPEDV-IPTIMLCPYPQSQSCWANSDIEEEMKQCQDIIKSIRSLR 939

Query: 904  K----ESRERRPGYVLPRNDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVV 963
                  + ++   Y+  +N    E   K    I  LA  S L V    ++ P GC V+VV
Sbjct: 940  ATYTIPASKKLHTYLHVKNQENLERFTKNSSFIRVLAYASELEVSISDESRP-GCIVNVV 999

Query: 964  NENLSVYLQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMN 1013
            NEN+S  L  +G +    E+ ++  K  ++ K  E L        Y +KVP +I ++N  
Sbjct: 1000 NENVSTLLDVRGSVDFNLEIARLETKKQQLVKNYETLVSKTTIPSY-DKVPQNIRDDNTQ 1057

BLAST of MS017846 vs. ExPASy Swiss-Prot
Match: Q04462 (Valine--tRNA ligase OS=Rattus norvegicus OX=10116 GN=Vars1 PE=2 SV=2)

HSP 1 Score: 984.6 bits (2544), Expect = 8.7e-286
Identity = 518/1037 (49.95%), Postives = 683/1037 (65.86%), Query Frame = 0

Query: 5    EKELKKKKALEK---ALKLQAQQTSNAPK--KSEKKNARRGGDEENAEDFVDPNTPSGEK 64
            +KE KK++ LEK     K Q QQ ++  K  K EKK  R  G         D  TP GEK
Sbjct: 235  KKEAKKREKLEKFQQKQKTQQQQPAHGEKKPKPEKKEKRDPG-----VITYDLPTPPGEK 294

Query: 65   KLLSQQMAKQYNPSAVEKSWYEWWEKSRFFVAD-------AKSSKPPFVIVLPPPNVTGA 124
            K +S  M   Y+P  VE +WY WWE+  FF  +       A + +  F++ +PPPNVTG+
Sbjct: 295  KDVSGTMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGS 354

Query: 125  LHIGHALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGR 184
            LH+GHALT AI+D++ RW RM G  TLW PG DHAGIATQVVVEKK+ +ER L RH +GR
Sbjct: 355  LHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGR 414

Query: 185  EKFISEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIY 244
            E F+ EVW WK + G  I  QL++LG+SLDW R CFTMD K S  VTEAFVRL + G+IY
Sbjct: 415  EAFLQEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIY 474

Query: 245  RDLRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELG-- 304
            R  RLVNW C L +AISDIEVD  ++  +TLL VPGY+  VEFGVL SFAY ++G     
Sbjct: 475  RSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDE 534

Query: 305  EIVVATTRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTG 364
            E+VVATTR+ETMLGD A+A+HP D RY+HL GK  +HPF  R LPI+ D   VD +FGTG
Sbjct: 535  EVVVATTRIETMLGDVAVAVHPKDPRYQHLKGKSVVHPFLSRSLPIVFDD-FVDMEFGTG 594

Query: 365  AVKITPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKK 424
            AVKITPAHD ND+EVG+RH LE I+I    G +  N    F GLPRF+AR+ V+ AL+++
Sbjct: 595  AVKITPAHDQNDYEVGQRHRLEAISIMDSKGAL-VNVPPPFLGLPRFEARKAVLAALKEQ 654

Query: 425  GLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQ 484
            GL+RG KDN M + LC+RS DVVEP+++PQWYV C +MA+ +  A T  + R   I+P+ 
Sbjct: 655  GLFRGIKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLR---ILPEA 714

Query: 485  YSADWKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYND--HWVVARSQEEA 544
            +   W  W++NIRDWCISRQLWWGHRIPA++ T+ D  +   G   D  +WV  R++ EA
Sbjct: 715  HQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPP-GEDPDGRYWVSGRTEAEA 774

Query: 545  EVEAKQIY--AGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGH 604
              +A + +  +  +  L QD DVLDTWFSSGLFP S+ GWP+ +EDL  FYP + LETGH
Sbjct: 775  REKAAREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGH 834

Query: 605  DILFFWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLE 664
            DILFFWVARMVMLG+ L   +PFR +YLH ++RDAHGRKMSKSLGNVIDP++VI+G+SL+
Sbjct: 835  DILFFWVARMVMLGLKLTEKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQ 894

Query: 665  GLHKRLEEGNLDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRV 724
            GLH +L   NLDP E+  AKEGQ  DFP GI ECG DALRF L +YT+Q   INLD+ R+
Sbjct: 895  GLHDQLLNSNLDPSEVEKAKEGQRADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRI 954

Query: 725  VGYRQWCNKLWNAIRFAMSKLGNDYVPSNNVTPD-TLPFSCQWILSVLNKAISRTVSSLE 784
            +GYR +CNKLWNA +FA+  LG  +VPS    P+       +WI S L +A+  +    +
Sbjct: 955  LGYRHFCNKLWNATKFALRGLGKGFVPSPTSKPEGHESLVDRWIRSRLAEAVRLSNEGFQ 1014

Query: 785  AYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLL 844
            AY+F   TTA YS+W Y+LCDV++E +KP  +  D    +A   A+ TL+ CLD GLRLL
Sbjct: 1015 AYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVD---QVAADCARQTLYTCLDVGLRLL 1074

Query: 845  HPFMPYVTEELWQRLPSPKSSARLESIMICNYPSVVE-EWTNDAVENEMDLVVSAVKSLR 904
             PFMP+VTEEL+QRLP    +A   S+ +  YP   E  W +   E  ++L +S  +++R
Sbjct: 1075 SPFMPFVTEELFQRLPRRTPNAP-ASLCVTPYPEPSECSWKDPEAEAALELALSITRAVR 1134

Query: 905  SLAKE---SRERRPGYVLPRNDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVS 964
            SL  +   +R R   ++   ++A     +     + TLA+   + V+     AP GCAV+
Sbjct: 1135 SLRADYNLTRTRPDCFLEVADEATGALASAVSAYVQTLASAGVVAVLALGAPAPQGCAVA 1194

Query: 965  VVNENLSVYLQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEEN 1019
            V ++  S++LQ QG++    EL K++ K  E ++Q +RL +   A+GY  KVP  + E +
Sbjct: 1195 VASDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASGYSAKVPLEVQEAD 1254

BLAST of MS017846 vs. ExPASy Swiss-Prot
Match: P49696 (Valine--tRNA ligase OS=Takifugu rubripes OX=31033 GN=vars1 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 2.5e-285
Identity = 503/1039 (48.41%), Postives = 691/1039 (66.51%), Query Frame = 0

Query: 5    EKELKKK-KALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFV--DPNTPSGEKKL 64
            E +LKK+ K  EK  K Q ++   A KK +    ++   E+     +  D  TPSGEKK 
Sbjct: 181  EAQLKKEAKKREKLEKFQQKKEMEAKKKMQPVAEKKAKPEKRELGVITYDIPTPSGEKKD 240

Query: 65   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVAD-------AKSSKPPFVIVLPPPNVTGALH 124
            +   +   Y+P  VE +WY WWEK  FF  +        ++ +  F++ +PPPNVTG+LH
Sbjct: 241  VVSPLPDSYSPQYVEAAWYPWWEKQGFFKPEFGRKSIGEQNPRGIFMMCIPPPNVTGSLH 300

Query: 125  IGHALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREK 184
            +GHALT AI+D + RW RM G  TLW PG DHAGIATQVVVEKK+MRE+  +RHD+GREK
Sbjct: 301  LGHALTNAIQDTLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLMREKGTSRHDLGREK 360

Query: 185  FISEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRD 244
            FI EVW WK + G  I  QL++LG+SLDW R CFTMD K S AV EAF+R+   G+IYR 
Sbjct: 361  FIEEVWKWKNEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSYAVQEAFIRMHDEGVIYRS 420

Query: 245  LRLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVV 304
             RLVNW C L +AISDIEVD  ++  +TLL VPGY+  VEFGVL SFAY ++G   E+VV
Sbjct: 421  KRLVNWSCSLNSAISDIEVDKNELSGRTLLPVPGYKEKVEFGVLVSFAYKVDGSDEEVVV 480

Query: 305  ATTRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKI 364
            ATTR+ETMLGDTA+A+HP D+RY+HL GK  +HPF  RK+P++ D   VD  FGTGAVKI
Sbjct: 481  ATTRIETMLGDTAVAVHPSDSRYQHLKGKTVLHPFCDRKIPVVFDD-FVDMSFGTGAVKI 540

Query: 365  TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYR 424
            TPAHD ND+EVG RHNL FINI  ++G +  N    F G+ RF AR+ V+ AL+ +  ++
Sbjct: 541  TPAHDHNDYEVGVRHNLAFINILDENGFV-INVPPPFLGMKRFDARKAVLQALKDRDQFK 600

Query: 425  GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSAD 484
              KDN M + +CSRS D+VEP++KPQWYV+C DM KQ+ DA  +    +++IIP  +S  
Sbjct: 601  EIKDNPMVVPVCSRSKDIVEPLMKPQWYVSCSDMGKQAADAVREG---RLKIIPDHHSQT 660

Query: 485  WKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHW-VVARSQEEAEVEAK 544
            W  W++NIRDWCISRQLWWGHRIPA++ T+ D  +K     + H+ V  R+ EEA  +A 
Sbjct: 661  WFNWMDNIRDWCISRQLWWGHRIPAYFITVSDPSVKPGEDMDGHYRVSGRTPEEAREKAA 720

Query: 545  QIY--AGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFF 604
            + +  +  +  L QD DVLDTWFSSG+ P S+LGWP++TEDL  FYP + LETGHDILFF
Sbjct: 721  KRFNVSPDKIALRQDEDVLDTWFSSGINPFSILGWPNETEDLNVFYPGTLLETGHDILFF 780

Query: 605  WVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKR 664
            WVARMVM+G+ L G +PF+ +Y   ++RDAHGRKMSKSLGNVIDP++   GI+LEGLH +
Sbjct: 781  WVARMVMMGLKLTGKLPFKEVYHCAVVRDAHGRKMSKSLGNVIDPLDDHIGIALEGLHAQ 840

Query: 665  LEEGNLDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQ 724
            L + NLDP E+   K+ Q  D+PN I ECG DALRFAL +YT+Q   INLD+ R++GYR 
Sbjct: 841  LMDTNLDPLEVEKPKKVQKADYPNCIPECGTDALRFALCAYTSQGRDINLDVNRILGYRH 900

Query: 725  WCNKLWNAIRFAMSKLGNDYVPSNNVTPDTLP------FSCQWILSVLNKAISRTVSSLE 784
            +CNKLWNA++FAM  LG+ +VP+     DT P       S +WILS L+ A+++  ++  
Sbjct: 901  FCNKLWNAVKFAMRTLGDQFVPA-----DTSPAEREESVSDRWILSRLSTAVAQCDAAFR 960

Query: 785  AYEFSDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHA--QDTLWLCLDNGLR 844
             Y+F   TTA+Y++W Y+LCDV++E++KP F     + +  R  A  + TL+ CL+ GLR
Sbjct: 961  TYDFPAITTAIYNFWLYELCDVYLESVKPVFIKAKEDGSCERPAAVCRQTLYTCLEVGLR 1020

Query: 845  LLHPFMPYVTEELWQRLPSPKSSARLESIMICNYPSVVEE-WTNDAVENEMDLVVSAVKS 904
            LL P MP+VTEEL+QRLP  +  +   SI +  YP   E  W  + V+ ++D ++  V++
Sbjct: 1021 LLAPLMPFVTEELYQRLPRRRPQSDPPSICVTPYPDAAEFCWQCEDVDRDIDFIMGVVRT 1080

Query: 905  LRSLAKESRERRPG---YVLPRNDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCA 964
            +RSL  + +  +     Y+   + A    V K  L+I TL+   ++  +     AP GCA
Sbjct: 1081 IRSLRSDYKLTKTAADCYLQCTDAATVSLVQKYSLQIQTLSYSQAIVPLMAPQPAPEGCA 1140

Query: 965  VSVVNENLSVYLQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHE 1019
            V++ ++  +V +  +G+I  E E+ K+  K  +++KQ E+L++ +    YKEKVP  + E
Sbjct: 1141 VAIASDRCTVNMMLKGLIDVEKEVPKLMGKKTDLEKQIEKLSEKISKGDYKEKVPVKVQE 1200

BLAST of MS017846 vs. ExPASy Swiss-Prot
Match: Q9Z1Q9 (Valine--tRNA ligase OS=Mus musculus OX=10090 GN=Vars1 PE=1 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 3.3e-285
Identity = 514/1035 (49.66%), Postives = 683/1035 (65.99%), Query Frame = 0

Query: 5    EKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFV-DPNTPSGEKKLLS 64
            +KE KK++ LEK    Q Q+T   P   EKK      ++ +      D  TP GEKK +S
Sbjct: 235  KKEAKKREKLEKF--QQKQKTQQQPPHGEKKPKPEKKEKRDPGVITYDLPTPPGEKKDVS 294

Query: 65   QQMAKQYNPSAVEKSWYEWWEKSRFFVAD-------AKSSKPPFVIVLPPPNVTGALHIG 124
              M   Y+P  VE +WY WWE+  FF  +       A + +  F++ +PPPNVTG+LH+G
Sbjct: 295  GAMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSLHLG 354

Query: 125  HALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 184
            HALT AI+D++ RW RM G  TLW PG DHAGIATQVVVEKK+ +ER L RH +GRE F+
Sbjct: 355  HALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFL 414

Query: 185  SEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLR 244
             EVW WK + G  I  QL++LG+SLDW R CFTMD K S  VTEAFVRL + G+IYR  R
Sbjct: 415  EEVWKWKAEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTR 474

Query: 245  LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELG--EIVV 304
            LVNW C L +AISDIEVD  ++  +TLL VPGY+  VEFGVL SFAY ++G     E+VV
Sbjct: 475  LVNWSCTLNSAISDIEVDKKELTGRTLLPVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVV 534

Query: 305  ATTRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKI 364
            ATTR+ETMLGD A+A+HP D RY+HL GK  +HPF  R LPI+ D   VD +FGTGAVKI
Sbjct: 535  ATTRIETMLGDVAVAVHPKDPRYQHLKGKCVVHPFLSRSLPIVFDD-FVDMEFGTGAVKI 594

Query: 365  TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYR 424
            TPAHD ND+EVG+RH LE I+I    G +  N    F GLPRF+AR+ V+ AL+++GL+R
Sbjct: 595  TPAHDQNDYEVGQRHRLEAISIMDSKGAL-INVPPPFLGLPRFEARKAVLAALKERGLFR 654

Query: 425  GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSAD 484
            G KDN M + LC+RS DVVEP+++PQWYV C +MA+ +  A T  + R   I+P+ +   
Sbjct: 655  GVKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLR---ILPEAHQRT 714

Query: 485  WKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYND--HWVVARSQEEAEVEA 544
            W  W++NIRDWCISRQLWWGHRIPA++ T+ D  +   G   D  +WV  R++ EA  +A
Sbjct: 715  WHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPP-GEDPDGRYWVSGRTEAEAREKA 774

Query: 545  KQIY--AGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILF 604
             + +  +  +  L QD DVLDTWFSSGLFP S+ GWP+ +EDL  FYP + LETGHDILF
Sbjct: 775  AREFGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILF 834

Query: 605  FWVARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHK 664
            FWVARMVMLG+ L G +PFR +YLH ++RDAHGRKMSKSLGNVIDP++VI+G+SL+GL+ 
Sbjct: 835  FWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLYD 894

Query: 665  RLEEGNLDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYR 724
            +L   NLDP E+  AKEGQ  DFP GI ECG DALRF L +YT+Q   INLD+ R++GYR
Sbjct: 895  QLLNSNLDPSEVEKAKEGQKADFPAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYR 954

Query: 725  QWCNKLWNAIRFAMSKLGNDYVPSNNVTPD-TLPFSCQWILSVLNKAISRTVSSLEAYEF 784
             +CNKLWNA +FA+  LG  +VPS    P+       +WI S L +A+  +    +AY+F
Sbjct: 955  HFCNKLWNATKFALRGLGKGFVPSATSKPEGHESLVDRWIRSRLTEAVRLSNEGFQAYDF 1014

Query: 785  SDATTAVYSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFM 844
               TTA YS+W Y+LCDV++E +KP  +  D    +A   A+ TL+ CLD GLRLL PFM
Sbjct: 1015 PAITTAQYSFWLYELCDVYLECLKPVLNGVD---QVAAECARQTLYTCLDVGLRLLSPFM 1074

Query: 845  PYVTEELWQRLP--SPKSSARLESIMICNYPSVVE-EWTNDAVENEMDLVVSAVKSLRSL 904
            P+VTEEL+QRLP  +PK+ A   S+ +  YP   E  W +   E  ++L +S  +++RSL
Sbjct: 1075 PFVTEELFQRLPRRTPKAPA---SLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSL 1134

Query: 905  AKE---SRERRPGYVLPRNDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVV 964
              +   +R R   ++   ++A     +     +  LA+   + V+     AP GCAV+V 
Sbjct: 1135 RADYNLTRTRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVAVA 1194

Query: 965  NENLSVYLQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMN 1019
            ++  S++LQ QG++    EL K++ K  E ++Q +RL +   A+ Y  KVP  + E +  
Sbjct: 1195 SDRCSIHLQLQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASSYSAKVPLEVQEADEA 1254

BLAST of MS017846 vs. ExPASy TrEMBL
Match: A0A6J1DPX4 (Valyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111023228 PE=3 SV=1)

HSP 1 Score: 2075.8 bits (5377), Expect = 0.0e+00
Identity = 1022/1025 (99.71%), Postives = 1023/1025 (99.80%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL
Sbjct: 21   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 80

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT
Sbjct: 81   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 140

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 141  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 200

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFK GLIYRDLRLVNWD
Sbjct: 201  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKRGLIYRDLRLVNWD 260

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKE TLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 261  CVLRTAISDIEVDYIDIKENTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 320

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN
Sbjct: 321  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 380

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM
Sbjct: 381  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 440

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN
Sbjct: 441  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 500

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF
Sbjct: 501  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 560

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI
Sbjct: 561  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 620

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 621  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 680

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 681  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 740

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ
Sbjct: 741  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 800

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP
Sbjct: 801  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 860

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
            SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR
Sbjct: 861  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 920

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA
Sbjct: 921  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 980

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH
Sbjct: 981  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1040

Query: 1022 IEAHK 1027
            IEAHK
Sbjct: 1041 IEAHK 1045

BLAST of MS017846 vs. ExPASy TrEMBL
Match: A0A6J1H3K8 (Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1)

HSP 1 Score: 1975.7 bits (5117), Expect = 0.0e+00
Identity = 961/1025 (93.76%), Postives = 996/1025 (97.17%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G+KKL
Sbjct: 82   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 141

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            +SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 142  MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 201

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 202  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 261

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 262  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 321

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 322  CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 381

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGK+AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDPN
Sbjct: 382  MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 441

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEF G+PRF+ RE VVDALQKKGLYRGAKDNEM
Sbjct: 442  DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFEVREMVVDALQKKGLYRGAKDNEM 501

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDA TDNE +KIEIIPKQYSADWKRWLEN
Sbjct: 502  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNECKKIEIIPKQYSADWKRWLEN 561

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVVAR++EEAE EAK IYAGK+F
Sbjct: 562  IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 621

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPD+TEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 622  HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 681

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 682  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 741

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 742  LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 801

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPS NVTPD LPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ
Sbjct: 802  FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 861

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTE+A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 862  YQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 921

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PKSS R ESIMIC+YPSV EEWTN++VE EMDL+VSAVKSLRSLAKESRERRPGYVLPR
Sbjct: 922  YPKSSTRPESIMICDYPSVTEEWTNESVETEMDLIVSAVKSLRSLAKESRERRPGYVLPR 981

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            ND V ET+NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSV+NENLSVYLQFQG ISAEA
Sbjct: 982  NDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVINENLSVYLQFQGAISAEA 1041

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI+KKM+EV+KQQE+L KMMDA+GYKEKV  HIHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 1042 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1101

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1102 IQAHR 1106

BLAST of MS017846 vs. ExPASy TrEMBL
Match: A0A6J1JYM1 (Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1)

HSP 1 Score: 1970.7 bits (5104), Expect = 0.0e+00
Identity = 959/1025 (93.56%), Postives = 995/1025 (97.07%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G+KKL
Sbjct: 83   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 142

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            +SQQM+KQYNPSAVEKSWYEWWEKS FFVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 143  MSQQMSKQYNPSAVEKSWYEWWEKSGFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 202

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD
Sbjct: 203  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 262

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 263  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 322

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLLKVPGYE PVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 323  CVLRTAISDIEVDYIDIKEKTLLKVPGYEKPVEFGVLTSFAYPLEGELGEIVVATTRVET 382

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIAIHPDDTRYKHLHGK+AIHPFNGRKLPIICDAILVDP FGTGAVKITPAHDPN
Sbjct: 383  MLGDTAIAIHPDDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPNFGTGAVKITPAHDPN 442

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEF G+PRFK RE VVDALQKKGLYRGAKDNEM
Sbjct: 443  DFEVGKRHNLEFINIFTDDGKINSNGGSEFEGMPRFKVREMVVDALQKKGLYRGAKDNEM 502

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDA TDNE +KI+IIPKQYSADWKRWLEN
Sbjct: 503  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAVTDNECKKIKIIPKQYSADWKRWLEN 562

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATL+DDQLKEFGAYNDHWVVAR++EEAE EAK IYAGK+F
Sbjct: 563  IRDWCISRQLWWGHRIPAWYATLDDDQLKEFGAYNDHWVVARNEEEAEEEAKLIYAGKKF 622

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPD+TEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 623  HLTQDPDVLDTWFSSGLFPLSVLGWPDNTEDLKAFYPTSALETGHDILFFWVARMVMLGI 682

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 683  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 742

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 743  LAVAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 802

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVPS NVTPD LPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVY WWQ
Sbjct: 803  FAMSKLGNDYVPSKNVTPDALPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYYWWQ 862

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSNDTE+A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 863  YQLCDVFIEAIKPYFSSNDTEYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 922

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PKSS R ESIMIC+YPSV EEWT+++VE EMDL+VSAVKSLRSLAKESRERRPGY+LPR
Sbjct: 923  YPKSSTRPESIMICDYPSVTEEWTSESVETEMDLIVSAVKSLRSLAKESRERRPGYMLPR 982

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            ND V ET+NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSVVNENLSVYLQFQG ISAEA
Sbjct: 983  NDVVAETINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 1042

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI+KKM+EV+KQQE+L KMMDA+GYKEKV  HIHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 1043 ELEKIKKKMEEVRKQQEKLKKMMDASGYKEKVRPHIHEENVNKLASLMQELLSLEEAGQH 1102

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1103 IQAHR 1107

BLAST of MS017846 vs. ExPASy TrEMBL
Match: A0A5D3BM83 (Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00290 PE=3 SV=1)

HSP 1 Score: 1963.0 bits (5084), Expect = 0.0e+00
Identity = 954/1025 (93.07%), Postives = 997/1025 (97.27%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G+KKL
Sbjct: 6    RAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 65

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            LSQQMAKQYNPSAVEKSWYEWWEKS +FVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 66   LSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 125

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW+
Sbjct: 126  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWE 185

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 186  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 245

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 246  CVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 305

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIA+HP+DTRYKHLHGK AIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN
Sbjct: 306  MLGDTAIAVHPEDTRYKHLHGKSAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 365

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEFAG+PRFKARE VVDALQKKGLYRGAKDNEM
Sbjct: 366  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNEM 425

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSR+NDVVEPMIKPQWYVNCK+ AKQSLDAA DNE +KI+IIPKQYSADWKRWL+N
Sbjct: 426  RLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNECKKIDIIPKQYSADWKRWLDN 485

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVAR++EEAE EAK+IYAGK+F
Sbjct: 486  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAEEEAKRIYAGKKF 545

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPDDTEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 546  HLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLGI 605

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 606  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 665

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 666  LAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 725

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVP+ NVTPD LPFSCQWILSVLNKAISRT+SSLE+YEFSDATTAVYSWWQ
Sbjct: 726  FAMSKLGNDYVPNKNVTPDVLPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWWQ 785

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSN+T++A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 786  YQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 845

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PK+S R ESIMIC+YPSV EEWTN+AVENEMDL+VSAV+SLRSLAKESRER+PGYVLPR
Sbjct: 846  YPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAVRSLRSLAKESRERKPGYVLPR 905

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            N AV E +NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSVVNENLSVYLQFQG ISAEA
Sbjct: 906  NLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 965

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI KK+DE+KKQQERL KMMDA+GYKEKV   IHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 966  ELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEENVNKLASLMQELLSLEEAGQH 1025

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1026 IQAHR 1030

BLAST of MS017846 vs. ExPASy TrEMBL
Match: A0A1S3CT04 (Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1)

HSP 1 Score: 1961.8 bits (5081), Expect = 0.0e+00
Identity = 952/1025 (92.88%), Postives = 997/1025 (97.27%), Query Frame = 0

Query: 2    QAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPSGEKKL 61
            +AREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDP+TP G+KKL
Sbjct: 21   KAREKELKKKKALEKALKLQAQQTSNAPKKSEKKNARRGGDEENAEDFVDPDTPFGKKKL 80

Query: 62   LSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIGHALTT 121
            LSQQMAKQYNPSAVEKSWYEWWEKS +FVADAKSSKPPFVIVLPPPNVTGALHIGHALT 
Sbjct: 81   LSQQMAKQYNPSAVEKSWYEWWEKSGYFVADAKSSKPPFVIVLPPPNVTGALHIGHALTA 140

Query: 122  AIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWD 181
            AIEDAIIRWRRMSG+NTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVW+
Sbjct: 141  AIEDAIIRWRRMSGFNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWE 200

Query: 182  WKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWD 241
            WKTKYGGTILKQLRRLGASLDW+RECFTMDEKRS+AVTEAFVRLFK+GLIYRDLRLVNWD
Sbjct: 201  WKTKYGGTILKQLRRLGASLDWTRECFTMDEKRSRAVTEAFVRLFKNGLIYRDLRLVNWD 260

Query: 242  CVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 301
            CVLRTAISDIEVDYIDIKEKTLL+VPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET
Sbjct: 261  CVLRTAISDIEVDYIDIKEKTLLRVPGYENPVEFGVLTSFAYPLEGELGEIVVATTRVET 320

Query: 302  MLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 361
            MLGDTAIA+HP+DTRYKHLHGK+AIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN
Sbjct: 321  MLGDTAIAVHPEDTRYKHLHGKFAIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPN 380

Query: 362  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGAKDNEM 421
            DFEVGKRHNLEFINIFTDDGKINSNGGSEFAG+PRFKARE VVDALQKKGLYRGAKDNEM
Sbjct: 381  DFEVGKRHNLEFINIFTDDGKINSNGGSEFAGMPRFKAREMVVDALQKKGLYRGAKDNEM 440

Query: 422  RLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLEN 481
            RLGLCSR+NDVVEPMIKPQWYVNCK+ AKQSLDAA DNE +KI+IIPKQYSADWKRWL+N
Sbjct: 441  RLGLCSRTNDVVEPMIKPQWYVNCKEPAKQSLDAAMDNECKKIDIIPKQYSADWKRWLDN 500

Query: 482  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIYAGKRF 541
            IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVAR++EEAE EAK+IYAGK+F
Sbjct: 501  IRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARNEEEAEEEAKRIYAGKKF 560

Query: 542  HLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGI 601
            HL QDPDVLDTWFSSGLFPLSVLGWPDDTEDLK FYPTSALETGHDILFFWVARMVMLGI
Sbjct: 561  HLTQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKAFYPTSALETGHDILFFWVARMVMLGI 620

Query: 602  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 661
            TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE
Sbjct: 621  TLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKE 680

Query: 662  LATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 721
            LA AKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR
Sbjct: 681  LAIAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIR 740

Query: 722  FAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQ 781
            FAMSKLGNDYVP+ N TPD LPFSCQWILSVLNKAISRT+SSLE+YEFSDATTAVYSWWQ
Sbjct: 741  FAMSKLGNDYVPNKNATPDVLPFSCQWILSVLNKAISRTISSLESYEFSDATTAVYSWWQ 800

Query: 782  YQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP 841
            YQLCDVFIEAIKPYFSSN+T++A ARSHAQDTLWLCL+NGLRLLHPFMPYVTEELWQRLP
Sbjct: 801  YQLCDVFIEAIKPYFSSNETDYASARSHAQDTLWLCLENGLRLLHPFMPYVTEELWQRLP 860

Query: 842  SPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRSLAKESRERRPGYVLPR 901
             PK+S R ESIMIC+YPSV EEWTN+AVENEMDL+VSAV+SLRSLAKESRER+PGYVLPR
Sbjct: 861  YPKNSTRPESIMICDYPSVTEEWTNEAVENEMDLIVSAVRSLRSLAKESRERKPGYVLPR 920

Query: 902  NDAVTETVNKRKLEIVTLANLSSLTVINDHDAAPVGCAVSVVNENLSVYLQFQGVISAEA 961
            N AV E +NKRKLEIVTLANLSSLTVIND+DAAPVGCAVSVVNENLSVYLQFQG ISAEA
Sbjct: 921  NLAVAEIINKRKLEIVTLANLSSLTVINDNDAAPVGCAVSVVNENLSVYLQFQGAISAEA 980

Query: 962  ELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQELLSLEEAGQH 1021
            ELEKI KK+DE+KKQQERL KMMDA+GYKEKV   IHEEN+NK+ASLMQELLSLEEAGQH
Sbjct: 981  ELEKINKKIDEIKKQQERLKKMMDASGYKEKVRPQIHEENVNKLASLMQELLSLEEAGQH 1040

Query: 1022 IEAHK 1027
            I+AH+
Sbjct: 1041 IQAHR 1045

BLAST of MS017846 vs. TAIR 10
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS) )

HSP 1 Score: 1597.8 bits (4136), Expect = 0.0e+00
Identity = 758/1034 (73.31%), Postives = 880/1034 (85.11%), Query Frame = 0

Query: 2    QAREKELKKKKALEK----ALKL-QAQQTSNAPKKSEKKNARRGGDEENAEDFVDPNTPS 61
            +A+EKELKK+KALEK     LK  QA+  +N PKKS KK+++R   EEN EDFVDP TP 
Sbjct: 67   KAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKKSSKRDASEENPEDFVDPETPL 126

Query: 62   GEKKLLSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHIG 121
            GE+K LS QMAKQY+P+ VEKSWY WWEKS  F ADAKSSKPPFVIVLPPPNVTGALHIG
Sbjct: 127  GERKRLSSQMAKQYSPATVEKSWYAWWEKSDLFKADAKSSKPPFVIVLPPPNVTGALHIG 186

Query: 122  HALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFI 181
            HALT+AIED IIRW+RMSGYN LWVPG DHAGIATQVVVEKKIMR+R +TRHD+GRE+F+
Sbjct: 187  HALTSAIEDTIIRWKRMSGYNALWVPGVDHAGIATQVVVEKKIMRDRGMTRHDVGREEFV 246

Query: 182  SEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLR 241
             EVW WK +YGGTIL QLRRLGASLDWSRECFTMDE+RSKAVTEAFVRL+K GLIYRD+R
Sbjct: 247  KEVWKWKNQYGGTILTQLRRLGASLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIR 306

Query: 242  LVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVAT 301
            LVNWDC+LRTAISD+EV+YIDIKEKTLLKVPGYE PVEFG+LTSFAYPLEG LGE++VAT
Sbjct: 307  LVNWDCILRTAISDVEVEYIDIKEKTLLKVPGYEKPVEFGLLTSFAYPLEGGLGEVIVAT 366

Query: 302  TRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFNGRKLPIICDAILVDPKFGTGAVKITP 361
            TRVETMLGDTAIAIHPDD RYKHLHGK+A+HPFNGRKLPIICD ILVDP FGTG VKITP
Sbjct: 367  TRVETMLGDTAIAIHPDDARYKHLHGKFAVHPFNGRKLPIICDGILVDPNFGTGCVKITP 426

Query: 362  AHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYRGA 421
            AHDPND EVGKRH LEFINIFTDDGKIN+NGGS+FAG+PRF ARE VV+ALQK+GLYRGA
Sbjct: 427  AHDPNDCEVGKRHKLEFINIFTDDGKINTNGGSDFAGMPRFAAREAVVEALQKQGLYRGA 486

Query: 422  KDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSADWK 481
            K+NEMRLGLCSR+NDV+EPMIKPQWYVNC  + K++LD A  +E++K+E +PKQY+A+W+
Sbjct: 487  KNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKEALDVAITDENKKLEFVPKQYTAEWR 546

Query: 482  RWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQIY 541
            RWLENIRDWCISRQLWWGHRIPAWYATLE+DQLKE GAY+DHWVVAR++++A  EA Q +
Sbjct: 547  RWLENIRDWCISRQLWWGHRIPAWYATLEEDQLKEVGAYSDHWVVARTEDDAREEAAQKF 606

Query: 542  AGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARM 601
             GK+F L +DPDVLDTWFSSGLFPLSVLGWPD T+D K FYPTS LETGHDILFFWVARM
Sbjct: 607  LGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVTDDFKAFYPTSVLETGHDILFFWVARM 666

Query: 602  VMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGN 661
            VM+G+ LGG+VPF  +Y HPMIRDAHGRKMSKSLGNVIDP+EVING++LEGLHKRLEEGN
Sbjct: 667  VMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKSLGNVIDPLEVINGVTLEGLHKRLEEGN 726

Query: 662  LDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKL 721
            LDPKE+  AKEGQVKDFPNGI ECG DALRFAL+SYTAQSDKINLDI RVVGYRQWCNKL
Sbjct: 727  LDPKEVIVAKEGQVKDFPNGIPECGTDALRFALVSYTAQSDKINLDILRVVGYRQWCNKL 786

Query: 722  WNAIRFAMSKLGNDYVPSNNVTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAV 781
            WNA+RFAM KLG+ Y P   ++P+T+PFSCQWILSVLNKAIS+TV SL+A+EFSDA   +
Sbjct: 787  WNAVRFAMMKLGDGYTPPQTLSPETMPFSCQWILSVLNKAISKTVVSLDAFEFSDAANTI 846

Query: 782  YSWWQYQLCDVFIEAIKPYFSSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEEL 841
            Y+WWQYQ CDV+IEAIKPYF+ ++  FA  R+HAQ  LW+ L+ GLRLLHPFMP+VTEEL
Sbjct: 847  YAWWQYQFCDVYIEAIKPYFAGDNPTFASERAHAQHALWISLETGLRLLHPFMPFVTEEL 906

Query: 842  WQRLPSPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVKSLRS-----LAKESR 901
            WQRLP+PK + R  SIMIC+YPS +E W+N+ VE+EMD V++ VK +R+     L K+  
Sbjct: 907  WQRLPAPKDTERKASIMICDYPSAIENWSNEKVESEMDTVLATVKCMRALRAGLLEKQKN 966

Query: 902  ERRPGYVLPRNDAVTETVNKRKLEIVTLANLSSLTVIN-DHDAAPVGCAVSVVNENLSVY 961
            ER P + L  N+  +E V   +LEI TLANLSSL V++    AAP G +V  VNENL VY
Sbjct: 967  ERLPAFALCENNVTSEIVKSHELEIRTLANLSSLEVVSKGQHAAPPGSSVETVNENLKVY 1026

Query: 962  LQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANGYKEKVPAHIHEENMNKVASLMQ 1021
            L+  G I+ EAE EKIR K+ E++KQ+E+L KMM  + Y+EKVPA+I E+N NK+A ++Q
Sbjct: 1027 LEVDGAINTEAEQEKIRNKIGELQKQKEKLQKMMSVSTYEEKVPANIKEDNANKLAKILQ 1086

Query: 1022 ELLSLEEAGQHIEA 1025
            E    E+    + A
Sbjct: 1087 EFDFFEKESARLAA 1100

BLAST of MS017846 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 643.7 bits (1659), Expect = 2.6e-184
Identity = 378/984 (38.41%), Postives = 546/984 (55.49%), Query Frame = 0

Query: 56   SGEKKLLSQQMAKQYNPSAVEKSWYEWWEKSRFFVADAKSSKPPFVIVLPPPNVTGALHI 115
            SG     S + +K ++ S+ EK  Y+WWE   +F  +      PFVI +PPPNVTG+LH+
Sbjct: 59   SGNNVFTSPETSKTFDFSSEEKI-YKWWESQGYFKPNFDQGGSPFVIPMPPPNVTGSLHM 118

Query: 116  GHALTTAIEDAIIRWRRMSGYNTLWVPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKF 175
            GHA+   +ED ++R+ RM+G  TLW+PGTDHAGIATQ+VVEK +  E  + R D+GR++F
Sbjct: 119  GHAMFVTLEDIMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASE-GIKRVDLGRDEF 178

Query: 176  ISEVWDWKTKYGGTILKQLRRLGASLDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDL 235
               VW+WK KYGGTI  Q++RLGAS DWSRE FT+DE+ S+AV EAFV+L   GLIY+  
Sbjct: 179  TKRVWEWKEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGS 238

Query: 236  RLVNWDCVLRTAISDIEVDYIDIKEKTLLKVPGYENPVEFGVLTSFAYPLEGELGEIVVA 295
             +VNW   L+TA+SD+EV+Y +                E G L    Y + G    + +A
Sbjct: 239  YMVNWSPNLQTAVSDLEVEYSE----------------EPGFLYHIKYRVAGSPDFLTIA 298

Query: 296  TTRVETMLGDTAIAIHPDDTRYKHLHGKYAIHPFN-GRKLPIICDAILVDPKFGTGAVKI 355
            TTR ET+ GD A+A+HP+D RY    G+ AI P   GR +PII D   VD  FGTG +KI
Sbjct: 299  TTRPETLFGDVALAVHPEDDRYSKYVGQTAIVPMTYGRHVPIIADK-YVDKDFGTGVLKI 358

Query: 356  TPAHDPNDFEVGKRHNLEFINIFTDDGKINSNGGSEFAGLPRFKARETVVDALQKKGLYR 415
            +P HD ND+ + ++  L  +N+   D  +N   G  F GL RF+ RE +   L++ GL  
Sbjct: 359  SPGHDHNDYLLARKLGLPILNVMNKDATLNDVAGL-FCGLDRFEVREKLWADLEEIGLAV 418

Query: 416  GAKDNEMRLGLCSRSNDVVEPMIKPQWYVNCKDMAKQSLDAATDNESRKIEIIPKQYSAD 475
              + + +R+    R  +V+EP++  QW+V+   +A+++L A    E++++ IIP+++   
Sbjct: 419  KKEPHTLRVPRSQRGGEVIEPLVSKQWFVHMDPLAEKALLAV---ENKELTIIPERFEKI 478

Query: 476  WKRWLENIRDWCISRQLWWGHRIPAWYATLEDDQLKEFGAYNDHWVVARSQEEAEVEAKQ 535
            +  WL NI+DWCISRQLWWGHRIP WY   +D +        + ++VA+S EEA  +A +
Sbjct: 479  YNHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCE--------EDYIVAKSAEEALEKALE 538

Query: 536  IYAGKRFHLAQDPDVLDTWFSSGLFPLSVLGWPD-DTEDLKTFYPTSALETGHDILFFWV 595
             Y GK   + QDPDVLDTWFSS L+P S LGWPD   +D   FYPT+ LETGHDILFFWV
Sbjct: 539  KY-GKDVEIYQDPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNMLETGHDILFFWV 598

Query: 596  ARMVMLGITLGGDVPFRTIYLHPMIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLE 655
            ARMVM+GI   G VPF  +YLH +IRD+ GRKMSKSLGNVIDP++ I             
Sbjct: 599  ARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTI------------- 658

Query: 656  EGNLDPKELATAKEGQVKDFPNGISECGADALRFALISYTAQSDKINLDIQRVVGYRQWC 715
                             KDF       G DALRF +   TA  D +NL  +R+   + + 
Sbjct: 659  -----------------KDF-------GTDALRFTIALGTAGQD-LNLSTERLTANKAFT 718

Query: 716  NKLWNAIRFAMSKLGN-----------DYVPSNNVTPDTLPFSCQWILSVLNKAISRTVS 775
            NKLWNA +F +  L +           D       T  +LP    W +S L+  I    +
Sbjct: 719  NKLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTA 778

Query: 776  SLEAYEFSDATTAVYSWWQYQLCDVFIEAIKP-YFSSNDTEFALARSHAQDTLWLCLDNG 835
            S E   F D     Y ++     D +IEA K   + S     +LA   +Q  L    +N 
Sbjct: 779  SYEKLFFGDVGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLA---SQAVLLYVFENI 838

Query: 836  LRLLHPFMPYVTEELWQRLPSPKSSARLESIMICNYPSVVEEWTNDAVENEMDLVVSAVK 895
            L+LLHPFMP+VTE+LWQ LP  K +             +V  W  +++   ++  +   +
Sbjct: 839  LKLLHPFMPFVTEDLWQALPYRKEAL------------IVSPWPQNSLPRNVE-SIKRFE 898

Query: 896  SLRSLAKESRERRPGYVLP----------RNDAVTETVNKRKLEIVTLANLSSLTVINDH 955
            +L++L +  R  R  Y +            +  V E ++K K E++ L +   L  ++  
Sbjct: 899  NLQALTRAIRNARAEYSVEPVKRISASVVGSAEVIEYISKEK-EVLALLSRLDLNNVHFS 951

Query: 956  DAAPVGCAVS---VVNENLSVYLQFQGVISAEAELEKIRKKMDEVKKQQERLTKMMDANG 1013
            +A P    +S   V +E L  YL    ++   +E+++I K++ +++ + + L   + +  
Sbjct: 959  NAPPGDANLSVHLVASEGLEAYLPLAAMVDISSEVQRISKRLSKMQTEYDALITRLSSPK 951

BLAST of MS017846 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-49
Identity = 193/858 (22.49%), Postives = 327/858 (38.11%), Query Frame = 0

Query: 83  WEKSRFF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTTAIEDAIIRWRRMSGYNTLW 142
           WE+++ F  V+D  ++   F++   PP   G LH+GHAL   ++D I R++ +  Y   +
Sbjct: 130 WEENQVFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQY 189

Query: 143 VPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGAS 202
           VPG D  G+  ++ V + + +E    R ++   K  ++   +      T ++  +R G  
Sbjct: 190 VPGWDCHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVW 249

Query: 203 LDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWDCVLRTAISDIEVDY----I 262
            DW+    T+D +   A  E F ++   G IYR  + V+W    RTA+++ E++Y    I
Sbjct: 250 ADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHI 309

Query: 263 DIKEKTLLKVPG-----------------------YENPVEFGV---------------- 322
                 + K+ G                       +  P    V                
Sbjct: 310 SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSF 369

Query: 323 ------LTSFAYPLEGELGE------IVVATTRVETMLGDTAIAIHPDDTRY-KHLHGKY 382
                 +TS    + G++ +      ++VAT  V  +     + +    T     L    
Sbjct: 370 SEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCR 429

Query: 383 AIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKIN 442
             HP + R  P++     +  + GTG V   P H   D+  G ++ L  ++   D+GK  
Sbjct: 430 YTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFT 489

Query: 443 SNGGSEFAGLPRF-KARETVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYV 502
              G +F GL    +    VV  L +       +    +     R+          QW+ 
Sbjct: 490 EEAG-QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFA 549

Query: 503 NCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYAT 562
           + +     ++DA  +     ++ +P Q          +  DWCISRQ  WG  IPA+Y  
Sbjct: 550 SVEGFRTATMDAINN-----VKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHV 609

Query: 563 LEDDQLKEFGAYND-HWVVARSQEEA------EVEAKQIYAGKRFHLAQDPDVLDTWFSS 622
              + L      N    ++++   +A      E    + Y  K     +  D +D WF S
Sbjct: 610 KTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDS 669

Query: 623 GLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHP 682
           G     VLG     E L   +P      G D    W    ++  I   G  P+  +  H 
Sbjct: 670 GSSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHG 729

Query: 683 MIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELATAKEGQVKDFPNG 742
            + D  G KMSKSLGNV+DP  VI G                            KD P  
Sbjct: 730 FVLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKDAP-- 789

Query: 743 ISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSNN 802
               GAD +R  + S     D + +  Q +        KL   +R+ +  L +D+   N 
Sbjct: 790 --AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWRVDNA 849

Query: 803 VTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYF 862
           V    LP   Q  L  L   +       E Y+F      +  +    L + + +  K   
Sbjct: 850 VPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRL 909

Query: 863 SSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP---SPKSSARLESIM 872
            +  T  +  R   Q  L   L + LR++ P +P++ E++WQ LP     +  +  E + 
Sbjct: 910 YTGGTS-SFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVF 941

BLAST of MS017846 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-49
Identity = 193/858 (22.49%), Postives = 327/858 (38.11%), Query Frame = 0

Query: 83  WEKSRFF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTTAIEDAIIRWRRMSGYNTLW 142
           WE+++ F  V+D  ++   F++   PP   G LH+GHAL   ++D I R++ +  Y   +
Sbjct: 130 WEENQVFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQY 189

Query: 143 VPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGAS 202
           VPG D  G+  ++ V + + +E    R ++   K  ++   +      T ++  +R G  
Sbjct: 190 VPGWDCHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVW 249

Query: 203 LDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWDCVLRTAISDIEVDY----I 262
            DW+    T+D +   A  E F ++   G IYR  + V+W    RTA+++ E++Y    I
Sbjct: 250 ADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHI 309

Query: 263 DIKEKTLLKVPG-----------------------YENPVEFGV---------------- 322
                 + K+ G                       +  P    V                
Sbjct: 310 SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSF 369

Query: 323 ------LTSFAYPLEGELGE------IVVATTRVETMLGDTAIAIHPDDTRY-KHLHGKY 382
                 +TS    + G++ +      ++VAT  V  +     + +    T     L    
Sbjct: 370 SEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCR 429

Query: 383 AIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKIN 442
             HP + R  P++     +  + GTG V   P H   D+  G ++ L  ++   D+GK  
Sbjct: 430 YTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFT 489

Query: 443 SNGGSEFAGLPRF-KARETVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYV 502
              G +F GL    +    VV  L +       +    +     R+          QW+ 
Sbjct: 490 EEAG-QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFA 549

Query: 503 NCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYAT 562
           + +     ++DA  +     ++ +P Q          +  DWCISRQ  WG  IPA+Y  
Sbjct: 550 SVEGFRTATMDAINN-----VKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHV 609

Query: 563 LEDDQLKEFGAYND-HWVVARSQEEA------EVEAKQIYAGKRFHLAQDPDVLDTWFSS 622
              + L      N    ++++   +A      E    + Y  K     +  D +D WF S
Sbjct: 610 KTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDS 669

Query: 623 GLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHP 682
           G     VLG     E L   +P      G D    W    ++  I   G  P+  +  H 
Sbjct: 670 GSSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHG 729

Query: 683 MIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELATAKEGQVKDFPNG 742
            + D  G KMSKSLGNV+DP  VI G                            KD P  
Sbjct: 730 FVLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKDAP-- 789

Query: 743 ISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSNN 802
               GAD +R  + S     D + +  Q +        KL   +R+ +  L +D+   N 
Sbjct: 790 --AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWRVDNA 849

Query: 803 VTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYF 862
           V    LP   Q  L  L   +       E Y+F      +  +    L + + +  K   
Sbjct: 850 VPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRL 909

Query: 863 SSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP---SPKSSARLESIM 872
            +  T  +  R   Q  L   L + LR++ P +P++ E++WQ LP     +  +  E + 
Sbjct: 910 YTGGTS-SFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVF 941

BLAST of MS017846 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-49
Identity = 193/858 (22.49%), Postives = 327/858 (38.11%), Query Frame = 0

Query: 83   WEKSRFF--VADAKSSKPPFVIVLPPPNVTGALHIGHALTTAIEDAIIRWRRMSGYNTLW 142
            WE+++ F  V+D  ++   F++   PP   G LH+GHAL   ++D I R++ +  Y   +
Sbjct: 316  WEENQVFKRVSD-NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQY 375

Query: 143  VPGTDHAGIATQVVVEKKIMRERNLTRHDIGREKFISEVWDWKTKYGGTILKQLRRLGAS 202
            VPG D  G+  ++ V + + +E    R ++   K  ++   +      T ++  +R G  
Sbjct: 376  VPGWDCHGLPIELKVLQSLDQE---VRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVW 435

Query: 203  LDWSRECFTMDEKRSKAVTEAFVRLFKSGLIYRDLRLVNWDCVLRTAISDIEVDY----I 262
             DW+    T+D +   A  E F ++   G IYR  + V+W    RTA+++ E++Y    I
Sbjct: 436  ADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHI 495

Query: 263  DIKEKTLLKVPG-----------------------YENPVEFGV---------------- 322
                  + K+ G                       +  P    V                
Sbjct: 496  SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSF 555

Query: 323  ------LTSFAYPLEGELGE------IVVATTRVETMLGDTAIAIHPDDTRY-KHLHGKY 382
                  +TS    + G++ +      ++VAT  V  +     + +    T     L    
Sbjct: 556  SEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCR 615

Query: 383  AIHPFNGRKLPIICDAILVDPKFGTGAVKITPAHDPNDFEVGKRHNLEFINIFTDDGKIN 442
              HP + R  P++     +  + GTG V   P H   D+  G ++ L  ++   D+GK  
Sbjct: 616  YTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFT 675

Query: 443  SNGGSEFAGLPRF-KARETVVDALQKKGLYRGAKDNEMRLGLCSRSNDVVEPMIKPQWYV 502
               G +F GL    +    VV  L +       +    +     R+          QW+ 
Sbjct: 676  EEAG-QFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFA 735

Query: 503  NCKDMAKQSLDAATDNESRKIEIIPKQYSADWKRWLENIRDWCISRQLWWGHRIPAWYAT 562
            + +     ++DA  +     ++ +P Q          +  DWCISRQ  WG  IPA+Y  
Sbjct: 736  SVEGFRTATMDAINN-----VKWVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHV 795

Query: 563  LEDDQLKEFGAYND-HWVVARSQEEA------EVEAKQIYAGKRFHLAQDPDVLDTWFSS 622
               + L      N    ++++   +A      E    + Y  K     +  D +D WF S
Sbjct: 796  KTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDS 855

Query: 623  GLFPLSVLGWPDDTEDLKTFYPTSALETGHDILFFWVARMVMLGITLGGDVPFRTIYLHP 682
            G     VLG     E L   +P      G D    W    ++  I   G  P+  +  H 
Sbjct: 856  GSSWAGVLG---KREGLS--FPADVYLEGTDQHRGWFQSSLLTSIATQGKAPYSAVITHG 915

Query: 683  MIRDAHGRKMSKSLGNVIDPVEVINGISLEGLHKRLEEGNLDPKELATAKEGQVKDFPNG 742
             + D  G KMSKSLGNV+DP  VI G                            KD P  
Sbjct: 916  FVLDEKGMKMSKSLGNVVDPRLVIEG------------------------GKNSKDAP-- 975

Query: 743  ISECGADALRFALISYTAQSDKINLDIQRVVGYRQWCNKLWNAIRFAMSKLGNDYVPSNN 802
                GAD +R  + S     D + +  Q +        KL   +R+ +  L +D+   N 
Sbjct: 976  --AYGADVMRLWVSSVDYTGD-VLIGPQILRQMSDIYRKLRGTLRYLLGNL-HDWRVDNA 1035

Query: 803  VTPDTLPFSCQWILSVLNKAISRTVSSLEAYEFSDATTAVYSWWQYQLCDVFIEAIKPYF 862
            V    LP   Q  L  L   +       E Y+F      +  +    L + + +  K   
Sbjct: 1036 VPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAKDRL 1095

Query: 863  SSNDTEFALARSHAQDTLWLCLDNGLRLLHPFMPYVTEELWQRLP---SPKSSARLESIM 872
             +  T  +  R   Q  L   L + LR++ P +P++ E++WQ LP     +  +  E + 
Sbjct: 1096 YTGGTS-SFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVF 1127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156305.10.0e+0099.71valine--tRNA ligase, mitochondrial 1 [Momordica charantia][more]
XP_038875929.10.0e+0093.66valine--tRNA ligase, mitochondrial 1 isoform X1 [Benincasa hispida][more]
XP_023533939.10.0e+0093.95valine--tRNA ligase, mitochondrial 1 [Cucurbita pepo subsp. pepo][more]
XP_022957954.10.0e+0093.76valine--tRNA ligase, mitochondrial 1 isoform X2 [Cucurbita moschata][more]
KAG6605705.10.0e+0093.76Valine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sor... [more]
Match NameE-valueIdentityDescription
P937360.0e+0073.31Valine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=TWN2 PE=... [more]
Q86KU26.6e-30250.92Probable valine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 G... [more]
Q044628.7e-28649.95Valine--tRNA ligase OS=Rattus norvegicus OX=10116 GN=Vars1 PE=2 SV=2[more]
P496962.5e-28548.41Valine--tRNA ligase OS=Takifugu rubripes OX=31033 GN=vars1 PE=3 SV=1[more]
Q9Z1Q93.3e-28549.66Valine--tRNA ligase OS=Mus musculus OX=10090 GN=Vars1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DPX40.0e+0099.71Valyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111023228 PE=3 SV=1[more]
A0A6J1H3K80.0e+0093.76Valyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459332 PE=3 SV=1[more]
A0A6J1JYM10.0e+0093.56Valyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490926 PE=3 SV=1[more]
A0A5D3BM830.0e+0093.07Valyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold17... [more]
A0A1S3CT040.0e+0092.88Valyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103504618 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G14610.10.0e+0073.31valyl-tRNA synthetase / valine--tRNA ligase (VALRS) [more]
AT5G16715.12.6e-18438.41ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... [more]
AT5G49030.11.1e-4922.49tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G49030.21.1e-4922.49tRNA synthetase class I (I, L, M and V) family protein [more]
AT5G49030.31.1e-4922.49tRNA synthetase class I (I, L, M and V) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 998..1018
NoneNo IPR availableCOILSCoilCoilcoord: 3..24
NoneNo IPR availableCOILSCoilCoilcoord: 956..986
NoneNo IPR availableGENE3D1.10.730.10coord: 707..890
e-value: 1.1E-50
score: 173.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..65
NoneNo IPR availablePANTHERPTHR11946:SF113BNAA08G31530D PROTEINcoord: 2..1018
NoneNo IPR availableCDDcd00817ValRS_corecoord: 98..701
e-value: 0.0
score: 573.424
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 65..711
IPR002303Valine-tRNA ligasePRINTSPR00986TRNASYNTHVALcoord: 428..441
score: 51.06
coord: 311..328
score: 57.2
coord: 100..111
score: 70.99
coord: 577..595
score: 67.84
coord: 546..567
score: 73.4
IPR002303Valine-tRNA ligaseTIGRFAMTIGR00422TIGR00422coord: 66..1004
e-value: 0.0
score: 1014.7
IPR002303Valine-tRNA ligasePANTHERPTHR11946VALYL-TRNA SYNTHETASEScoord: 2..1018
IPR002303Valine-tRNA ligaseHAMAPMF_02004Val_tRNA_synth_type1coord: 66..1023
score: 13.893896
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 447..700
e-value: 1.1E-35
score: 125.2
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 67..265
e-value: 7.4E-65
score: 221.5
IPR037118Valyl-tRNA synthetase, tRNA-binding arm superfamilyGENE3D1.10.287.380coord: 951..1022
e-value: 5.1E-8
score: 34.8
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 79..702
e-value: 5.4E-202
score: 672.3
IPR013155Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-bindingPFAMPF08264Anticodon_1coord: 747..889
e-value: 6.2E-36
score: 123.7
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainGENE3D3.90.740.10coord: 274..418
e-value: 4.4E-50
score: 171.1
IPR009008Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domainSUPERFAMILY50677ValRS/IleRS/LeuRS editing domaincoord: 273..423
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 107..118
IPR033705Valyl tRNA synthetase, anticodon-binding domainCDDcd07962Anticodon_Ia_Valcoord: 701..840
e-value: 3.71334E-55
score: 185.453
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 713..972

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017846.1MS017846.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0006438 valyl-tRNA aminoacylation
molecular_function GO:0002161 aminoacyl-tRNA editing activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0004832 valine-tRNA ligase activity