MS017770 (gene) Bitter gourd (TR) v1

Overview
NameMS017770
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCytochrome p450
Locationscaffold373: 2394670 .. 2397012 (-)
RNA-Seq ExpressionMS017770
SyntenyMS017770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAGCAATAATGAGTTTACTTATTTTGTTGGTGTTGTGGGTATTGTTTTGCTGTTGGATTTCGCCCTTTTTGGCGTACAATAAACTGAAGAGAAATGGGTTTGGAGGTCCAACGCCAAGTTTTCCTCTTGGAAATATTAGTGAGATGAAAAAGAAGAACAAAAATTGTTCTGTATTAGAATCTTCTCAAGTAACCCACAACATACATTCCACTGTGTTTCCTTATTTTTCTCGCTGGCAAGCTTCCCATGGTAAGCCTTAATAAACAAAACAAAAAAAAACCTTTTTTTTTTCTTGTCTTTTGGCTTCGATACCAAAAACTTTGTTTAATTTGGTGTGTGTTTTGTTTAATGTTGTCACTCAGGGAAAATATTTATATATTGGTTGGGGACAGAGCCGTTCTTGTACATTGCAGAGCCAGAATTTTTGAAGACATTGTCGACAGAAGTGAAGGCCAAGTTTTGGGGAAAGCCTTCTGTGTTCAAAGATGATAGGAAATCAATGTTTGGAAATGGTTTGGTGATGGCTGAAGGTGATCAGTGGGTTCGCCAAAGGCATGTTATTACTCCTGCATTCAATCCATCCAACTTGAAGGTAATTAATTAATTTGTAATCTAAAAATTAATTAAATTAATTTGTTCAAACACAACCCTTTTTCTTTCTTTCTAATTTTCTAGTTGTTTCGAATTAGCTCTCAACCAACCCTCAAGTTTGTGTTTATGTTTTAAAAAAGTCTAAAATAGTTATTTTAACTTTCAATTTTGTATTTAATAAGTTGTTCTATGTCAATTTCTATCACTATGCTATTAATACATTATTTGACTATTAGATTTTGGGTAGATTCAATCATTTGATTATATATAAATAAATTTAAACACTTCCACTCACTTAAAAGTTAATAGATTTTATTAGAAACTTTTTAAAATACAAAAATTGGAATGTTAGGACCAAATTTGTAATAATAAAGTTTCTCATATAATATTAAAAAATATTATCCGATCGAAAGTTTGATAAATGGGAATATATATTATATGTATAGGCGATGGGAAGCTTAATGGTGGAGTCGACCACCGAAATGCTGGAGAGGTGGGCCCACATGATAAACTCCGGCGACCGGGAAATCGAAGTGGAGCGAGAGATCACGGCGACGGCCGGCGAGATCATCGCGAAAACCAGCTTCGGAATCGACCACCGGAGCGGAAGGCAAGTGTTCGATAAACTGAGGGCTCTTCAGATGACACTCTTCAAAACGAACCGCCTCGTCGGCGTTCCGTTCGGCCGTCTACTTAACGCCGGCAAGGCGAGGGAGGCGGAGCGGCTCGGGGAGGAAATAGACGGCCTTTTCGAGTCCGTGATAACGGCGAGAAGGGAGGGGAATAATAATCAGGTGCAGCAGGATCTACTGAGCTTGCTTTTGAGAGACGACGGCGGGAGGAGATTGAGTACGACGGAGCTGGTTGACGAGTGCAAGACGTTCTTCTTCGGAGGGCATGAAACGACGGCGTTGGCTGTGACGTGGACTCTGCTGCTTTTGGGTATCCATACAGAGTGGCAAACGCAGCTGAGAGAAGAGGTTAAGGAAGTTCTTGGAAATAAAGAGAGTGAATTTGATTTCTCCAACCTTGCCGGACTCAAGAAGGTAAAATCACTTCAAATTCACCTTTTTTTGGGGTGAAATATTTTCTACTTTTTCACTCTTCCTTTTTTATTCTTTTTTCTTTATTAATGCACAACAAATAATTGATTTAAATATAGGCATTACATAGGGTAATAATTGATTTAAAAGTATGATATGACAAGATGAGAGTGTTACACAATAATACACCAAAGTTTCCTAGAAATCTTTCATGGTAATTAATTGTGAAACATTAATAAATCCAAAAGCTGACAAGACATTGGAAAAAAAATGCAGATGGGATGGGTGATGAGGGAGGTCCTCCGTCTGTACCCATCAGCACCAAACGTCCAAAGGCAAGCCAGAAAAGACATCACCATAAACGGCCTCACCATCCCCGACGGCACCAACATGTGGATCGATGTCGTGGCGATGCACCATGACGAGGCCCTTTGGGGTGACAAAGTCAACGAATTTCATCCCGAAAGGTTCGAAAACGATGCTGTTGCTGGTGGCTGCACCCACAAGATGGGGTACCTACCCTTCGGGTTCGGAGGGAGAATGTGCGTCGGAAGGCATTTGACGTTTATGGAGTATAAGATTATGGTGGCACTGATTTTGTGTAGGTTTTCCTTCTCTCTTTCTCCTGTTTATCGCCACTCGCCTTCGATTATGCTATCGCTCCGACCTGCTCACGGGTTGCCCCTCATATTCCAACCGCTGGAAACT

mRNA sequence

TTAGCAATAATGAGTTTACTTATTTTGTTGGTGTTGTGGGTATTGTTTTGCTGTTGGATTTCGCCCTTTTTGGCGTACAATAAACTGAAGAGAAATGGGTTTGGAGGTCCAACGCCAAGTTTTCCTCTTGGAAATATTAGTGAGATGAAAAAGAAGAACAAAAATTGTTCTGTATTAGAATCTTCTCAAGTAACCCACAACATACATTCCACTGTGTTTCCTTATTTTTCTCGCTGGCAAGCTTCCCATGGGAAAATATTTATATATTGGTTGGGGACAGAGCCGTTCTTGTACATTGCAGAGCCAGAATTTTTGAAGACATTGTCGACAGAAGTGAAGGCCAAGTTTTGGGGAAAGCCTTCTGTGTTCAAAGATGATAGGAAATCAATGTTTGGAAATGGTTTGGTGATGGCTGAAGGTGATCAGTGGGTTCGCCAAAGGCATGTTATTACTCCTGCATTCAATCCATCCAACTTGAAGGCGATGGGAAGCTTAATGGTGGAGTCGACCACCGAAATGCTGGAGAGGTGGGCCCACATGATAAACTCCGGCGACCGGGAAATCGAAGTGGAGCGAGAGATCACGGCGACGGCCGGCGAGATCATCGCGAAAACCAGCTTCGGAATCGACCACCGGAGCGGAAGGCAAGTGTTCGATAAACTGAGGGCTCTTCAGATGACACTCTTCAAAACGAACCGCCTCGTCGGCGTTCCGTTCGGCCGTCTACTTAACGCCGGCAAGGCGAGGGAGGCGGAGCGGCTCGGGGAGGAAATAGACGGCCTTTTCGAGTCCGTGATAACGGCGAGAAGGGAGGGGAATAATAATCAGGTGCAGCAGGATCTACTGAGCTTGCTTTTGAGAGACGACGGCGGGAGGAGATTGAGTACGACGGAGCTGGTTGACGAGTGCAAGACGTTCTTCTTCGGAGGGCATGAAACGACGGCGTTGGCTGTGACGTGGACTCTGCTGCTTTTGGGTATCCATACAGAGTGGCAAACGCAGCTGAGAGAAGAGGTTAAGGAAGTTCTTGGAAATAAAGAGAACATTGGAAAAAAAATGCAGATGGGATGGGTGATGAGGGAGGTCCTCCGTCTGTACCCATCAGCACCAAACGTCCAAAGGCAAGCCAGAAAAGACATCACCATAAACGGCCTCACCATCCCCGACGGCACCAACATGTGGATCGATGTCGTGGCGATGCACCATGACGAGGCCCTTTGGGGTGACAAAGTCAACGAATTTCATCCCGAAAGGTTCGAAAACGATGCTGTTGCTGGTGGCTGCACCCACAAGATGGGGTACCTACCCTTCGGGTTCGGAGGGAGAATGTGCGTCGGAAGGCATTTGACGTTTATGGAGTATAAGATTATGGTGGCACTGATTTTGTGTAGGTTTTCCTTCTCTCTTTCTCCTGTTTATCGCCACTCGCCTTCGATTATGCTATCGCTCCGACCTGCTCACGGGTTGCCCCTCATATTCCAACCGCTGGAAACT

Coding sequence (CDS)

TTAGCAATAATGAGTTTACTTATTTTGTTGGTGTTGTGGGTATTGTTTTGCTGTTGGATTTCGCCCTTTTTGGCGTACAATAAACTGAAGAGAAATGGGTTTGGAGGTCCAACGCCAAGTTTTCCTCTTGGAAATATTAGTGAGATGAAAAAGAAGAACAAAAATTGTTCTGTATTAGAATCTTCTCAAGTAACCCACAACATACATTCCACTGTGTTTCCTTATTTTTCTCGCTGGCAAGCTTCCCATGGGAAAATATTTATATATTGGTTGGGGACAGAGCCGTTCTTGTACATTGCAGAGCCAGAATTTTTGAAGACATTGTCGACAGAAGTGAAGGCCAAGTTTTGGGGAAAGCCTTCTGTGTTCAAAGATGATAGGAAATCAATGTTTGGAAATGGTTTGGTGATGGCTGAAGGTGATCAGTGGGTTCGCCAAAGGCATGTTATTACTCCTGCATTCAATCCATCCAACTTGAAGGCGATGGGAAGCTTAATGGTGGAGTCGACCACCGAAATGCTGGAGAGGTGGGCCCACATGATAAACTCCGGCGACCGGGAAATCGAAGTGGAGCGAGAGATCACGGCGACGGCCGGCGAGATCATCGCGAAAACCAGCTTCGGAATCGACCACCGGAGCGGAAGGCAAGTGTTCGATAAACTGAGGGCTCTTCAGATGACACTCTTCAAAACGAACCGCCTCGTCGGCGTTCCGTTCGGCCGTCTACTTAACGCCGGCAAGGCGAGGGAGGCGGAGCGGCTCGGGGAGGAAATAGACGGCCTTTTCGAGTCCGTGATAACGGCGAGAAGGGAGGGGAATAATAATCAGGTGCAGCAGGATCTACTGAGCTTGCTTTTGAGAGACGACGGCGGGAGGAGATTGAGTACGACGGAGCTGGTTGACGAGTGCAAGACGTTCTTCTTCGGAGGGCATGAAACGACGGCGTTGGCTGTGACGTGGACTCTGCTGCTTTTGGGTATCCATACAGAGTGGCAAACGCAGCTGAGAGAAGAGGTTAAGGAAGTTCTTGGAAATAAAGAGAACATTGGAAAAAAAATGCAGATGGGATGGGTGATGAGGGAGGTCCTCCGTCTGTACCCATCAGCACCAAACGTCCAAAGGCAAGCCAGAAAAGACATCACCATAAACGGCCTCACCATCCCCGACGGCACCAACATGTGGATCGATGTCGTGGCGATGCACCATGACGAGGCCCTTTGGGGTGACAAAGTCAACGAATTTCATCCCGAAAGGTTCGAAAACGATGCTGTTGCTGGTGGCTGCACCCACAAGATGGGGTACCTACCCTTCGGGTTCGGAGGGAGAATGTGCGTCGGAAGGCATTTGACGTTTATGGAGTATAAGATTATGGTGGCACTGATTTTGTGTAGGTTTTCCTTCTCTCTTTCTCCTGTTTATCGCCACTCGCCTTCGATTATGCTATCGCTCCGACCTGCTCACGGGTTGCCCCTCATATTCCAACCGCTGGAAACT

Protein sequence

LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFGRLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKENIGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLET
Homology
BLAST of MS017770 vs. NCBI nr
Match: XP_022159617.1 (cytokinin hydroxylase-like [Momordica charantia])

HSP 1 Score: 983.8 bits (2542), Expect = 5.5e-283
Identity = 488/504 (96.83%), Postives = 491/504 (97.42%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE
Sbjct: 8   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 67

Query: 61  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 120
           SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP
Sbjct: 68  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 127

Query: 121 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 180
           SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWA M
Sbjct: 128 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWARM 187

Query: 181 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFG 240
           INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVF+KLRALQMTLFKTNRLVGVPFG
Sbjct: 188 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFEKLRALQMTLFKTNRLVGVPFG 247

Query: 241 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELV 300
           RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELV
Sbjct: 248 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELV 307

Query: 301 DECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKE------NIGKKMQ 360
           DECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKE      N+    +
Sbjct: 308 DECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKESEFDFSNLAGLKK 367

Query: 361 MGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEF 420
           MGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEF
Sbjct: 368 MGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEF 427

Query: 421 HPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPVYR 480
           HPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSP YR
Sbjct: 428 HPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPAYR 487

Query: 481 HSPSIMLSLRPAHGLPLIFQPLET 499
           HSPSIMLSLRPAHGLPLIFQ LET
Sbjct: 488 HSPSIMLSLRPAHGLPLIFQLLET 511

BLAST of MS017770 vs. NCBI nr
Match: XP_022958036.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 793.9 bits (2049), Expect = 8.1e-226
Identity = 400/514 (77.82%), Postives = 445/514 (86.58%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           L IM+ L  L+  +LF CWISPFLA+ KLKRNGF GPTPSFPLGNISEMK+ + +  V E
Sbjct: 9   LVIMAFLTSLLFKILFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFD-RVFE 68

Query: 61  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 120
           S  +TH+IHS VFPYFSRWQA+HGK+F YWLGTEPFLY+AEPEF+K LS EV+AK+WGKP
Sbjct: 69  S--LTHDIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKMLSREVQAKYWGKP 128

Query: 121 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 180
           SVFK DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVESTT+ML+RWAH+
Sbjct: 129 SVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHL 188

Query: 181 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFG 240
           + SG  +IEVE EIT+TAGEIIAKTSFGIDH SGRQVF KLR LQMTLFKTNRLVGVPF 
Sbjct: 189 VLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFA 248

Query: 241 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQV------QQDLLSLLLRDDG---- 300
            +LNAGKAREA+RLGEEID LF  VITARRE   NQ       Q DLLSLLL++ G    
Sbjct: 249 GILNAGKAREAKRLGEEIDELFREVITARRE---NQAAAEVVQQNDLLSLLLKESGGEGK 308

Query: 301 -GRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKEN- 360
            GR LSTTEL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQTQLR+E+KEVLG+KE+ 
Sbjct: 309 LGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESD 368

Query: 361 -----IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHD 420
                + +  +MGWVM EVLRLYPSAPNVQRQAR+DIT+NGLTIP+GTNMWIDVVAMHHD
Sbjct: 369 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHD 428

Query: 421 EALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILC 480
           EALWG++VNEF PERFE+D VAGGC+HKMGYLPFGFGGRMCVGRHLTFMEYKI++ LILC
Sbjct: 429 EALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILC 488

Query: 481 RFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           RFSF+LSP Y HSP IMLSLRPAHGLPLIFQ LE
Sbjct: 489 RFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 516

BLAST of MS017770 vs. NCBI nr
Match: XP_023533013.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.0 bits (2026), Expect = 3.7e-223
Identity = 395/512 (77.15%), Postives = 438/512 (85.55%), Query Frame = 0

Query: 4   MSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLESSQ 63
           M+    L+  +LF CWISPFLA+ KLKRNGF GPTPSFPLGNISEMK+ +    V ES  
Sbjct: 1   MAFFTSLLFMLLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFG-RVFES-- 60

Query: 64  VTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPSVF 123
           +TH+IHS VFPYFSRWQA+HGKIF YWLGTEPFLYIAEPEF++ LS EV+AK+WGKPSVF
Sbjct: 61  LTHDIHSIVFPYFSRWQAAHGKIFTYWLGTEPFLYIAEPEFVRMLSREVQAKYWGKPSVF 120

Query: 124 KDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHMINS 183
           K DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVESTT+ML+RWAH++ S
Sbjct: 121 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 180

Query: 184 GDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFGRLL 243
           G  +IEVE EIT+TAGEIIAKTSFGIDH SGRQVF KLR LQMTLFKTNRLVGVPF  +L
Sbjct: 181 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGIL 240

Query: 244 NAGKAREAERLGEEIDGLFESVITARREGNNNQV-------QQDLLSLLLRDDG-----G 303
            AGKAREA+RLGEEID LF  VITARRE             Q DLLSLLL++ G     G
Sbjct: 241 IAGKAREAKRLGEEIDELFREVITARRENQAAAAAAAEVVQQNDLLSLLLKESGGEGKLG 300

Query: 304 RRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKEN--- 363
           R LSTTEL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQTQLR+E+KEVLG+KE+   
Sbjct: 301 RWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESDFD 360

Query: 364 ---IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEA 423
              + +  +MGWVM EVLRLYPSAPNVQRQAR+DIT+NGLTIP+GTNMWIDVVAMHHDEA
Sbjct: 361 FTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHDEA 420

Query: 424 LWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRF 483
           LWG++VNEF PERFE+D VAGGC+HKMGYLPFGFGGRMCVGRHLTFMEYKI++ LILCRF
Sbjct: 421 LWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILCRF 480

Query: 484 SFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           SF+LSP Y HSP IMLSLRPAHGLPLIFQ LE
Sbjct: 481 SFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 509

BLAST of MS017770 vs. NCBI nr
Match: XP_022995689.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-222
Identity = 394/514 (76.65%), Postives = 441/514 (85.80%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           L IM+  I L+  VLF CWISPFLA+ KLKRNGF GPTPSFPLGNISEMK+ +    V E
Sbjct: 9   LVIMAFFISLLFKVLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFG-RVFE 68

Query: 61  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 120
              +TH+IHS VFPYFSRWQA++GKIF YWLGTEPFLY+A+PEF+K LS EV+AK+WGKP
Sbjct: 69  C--LTHDIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKP 128

Query: 121 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 180
           SVFK DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVESTT+ML+RWAH+
Sbjct: 129 SVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHL 188

Query: 181 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFG 240
           + SG  +IEVE EIT+TAGEIIAKTSFGIDH SGRQVF KLR LQMTLFKTNRLVGVPF 
Sbjct: 189 VLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFA 248

Query: 241 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQ------VQQDLLSLLLRDDGG--- 300
            +LNAGKAREA+RLGEEID LF  VITARRE  +        +Q DLLSLLL++ GG   
Sbjct: 249 GILNAGKAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKESGGEGK 308

Query: 301 --RRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKEN- 360
             R LST EL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQ QLR+E+KEVLG+KE+ 
Sbjct: 309 LRRWLSTAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESD 368

Query: 361 -----IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHD 420
                + +  +MGWVM EVLRLYPSAPNVQRQAR+DIT+NGLTIP+GTNMWIDVVAMHHD
Sbjct: 369 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHD 428

Query: 421 EALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILC 480
           EALWG++VNEF PERFE+D VAGGC+HKMGYLPFGFGGRMCVGRHLTFMEYKI++ LILC
Sbjct: 429 EALWGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILC 488

Query: 481 RFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           RFSF+LSP Y HSP IMLSLRPAHGLPLIF+ LE
Sbjct: 489 RFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLE 519

BLAST of MS017770 vs. NCBI nr
Match: XP_038875746.1 (LOW QUALITY PROTEIN: cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 770.4 bits (1988), Expect = 9.5e-219
Identity = 381/509 (74.85%), Postives = 432/509 (84.87%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           L I++L IL    VLFCCW+SPFL + KLKRNGF GPTPSFPLGNISEMKKKN N +  E
Sbjct: 11  LVIIALFILFAFRVLFCCWVSPFLVHKKLKRNGFRGPTPSFPLGNISEMKKKNVNPNTFE 70

Query: 61  SS--QVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWG 120
            S  QVTH+IHS VFPYF+RWQ ++GK+F YWLGTEPFLYI EPE +K LS EVKAK+WG
Sbjct: 71  ESSHQVTHDIHSIVFPYFARWQTTYGKVFTYWLGTEPFLYIGEPEIVKILSKEVKAKYWG 130

Query: 121 KPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWA 180
           KPSVFK+DRKSMFGNGLVMAEGD+WVRQRH+ITPAFNPSNLKAM SLM ES T+M+ERWA
Sbjct: 131 KPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMAESATQMVERWA 190

Query: 181 HMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVP 240
           +++ SG+  IEVE+EIT+TAGEIIAKTSFGI H SGRQVF+KLR LQ TLFKTNRLVGVP
Sbjct: 191 NLVISGNPXIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRHLQTTLFKTNRLVGVP 250

Query: 241 FGRLLNAGKAREAERLGEEIDGLFESVITARRE---GNNNQVQQDLLSLLLRDDGGRRLS 300
           F  +LNA KAR+A+RLG+EID LF SVI  RRE       + Q DLLSLLL+ +G R LS
Sbjct: 251 FAGVLNAAKARQAKRLGKEIDALFMSVIKERRENAAAAAGEQQNDLLSLLLK-EGERALS 310

Query: 301 TTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNK-------EN 360
           TTEL+DECKTFFFGGHETTALA+TWTL+LL IH+EWQT+LREE+KEV G+         N
Sbjct: 311 TTELIDECKTFFFGGHETTALALTWTLMLLAIHSEWQTKLREEIKEVFGDNIQTHFDFTN 370

Query: 361 IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWG 420
           +    +MGWVM EVLRLYPSAPNVQRQARKDITINGLTIP+GTN+WIDVVAMHHD+ALWG
Sbjct: 371 LASLKKMGWVMSEVLRLYPSAPNVQRQARKDITINGLTIPNGTNIWIDVVAMHHDQALWG 430

Query: 421 DKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFS 480
            +VNEFHPERF+ND V+GGCTHKMGYLPFGFGGRMCVGRHLTFMEYKI++  IL RFSFS
Sbjct: 431 HQVNEFHPERFQNDVVSGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTHILSRFSFS 490

Query: 481 LSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           LS  YRHSPSIMLSLRP HGLPL+F+ L+
Sbjct: 491 LSSDYRHSPSIMLSLRPIHGLPLVFRLLD 518

BLAST of MS017770 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.0e-86
Identity = 184/509 (36.15%), Postives = 279/509 (54.81%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCW-ISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVL 60
           L I+  LIL VL+   CC+ ++P      ++R G  GP P    GNI ++ K   + +  
Sbjct: 11  LVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASN 70

Query: 61  ESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGK 120
           + S + HNI   + P++  W   +GK FI W GTEP L + E E +K L T+      GK
Sbjct: 71  DCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTK-HNPVTGK 130

Query: 121 PSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAH 180
             + +   K   G GL+MA G+ W  QRH+  PAF    LK     MVE T  M ER   
Sbjct: 131 SWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRK 190

Query: 181 MINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPF 240
            +     E+E+  E+     +II++T FG     G+++F  L  LQ    +  R +  P 
Sbjct: 191 EVG---EEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPG 250

Query: 241 GRLLNAGKAREAERLGEEIDGLFESVITARRE----GNNNQVQQDLLSLLLR--DDGGRR 300
            R L +   RE + L  E++ L   +I +R++    G ++    DLL LLL   D     
Sbjct: 251 SRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKNN 310

Query: 301 LSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLG-----NKEN 360
           L+   ++DECKTFFF GHETT+L +TWTL+LL  +  WQ  +R+EV++V G     + E 
Sbjct: 311 LNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQ 370

Query: 361 IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWG 420
           +     +  V+ E LRLYP A  + R A +DI +  L IP G ++WI V+A+HH   LWG
Sbjct: 371 LSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWG 430

Query: 421 DKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFS 480
           +  NEF+PERF   + A        ++PF  G R C+G+    ME KI++A+++ +FSF+
Sbjct: 431 EDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFA 490

Query: 481 LSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           +S  YRH+P ++L+++P +G+ L+ +PL+
Sbjct: 491 ISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of MS017770 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.9e-85
Identity = 179/515 (34.76%), Postives = 280/515 (54.37%), Query Frame = 0

Query: 1   LAIMSLLILLVLW-VLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVL 60
           L I    IL VL+  + C W++P      +++ G  GP P    GNI E+       +  
Sbjct: 10  LVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASK 69

Query: 61  ESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTE---VKAKF 120
           +   + H+I   + P++  W   +GK FI W GT+P L + E E +K L  +   V  + 
Sbjct: 70  DCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRS 129

Query: 121 WGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLER 180
           W    + +   K+  G GL+MA G  W  QRH+  PAF    LK     MVE T++++ER
Sbjct: 130 W----LQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVER 189

Query: 181 WAHMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVG 240
               +  G  E+E+  E+     +II++T FG     G+++F+ L  LQ    +  R + 
Sbjct: 190 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 249

Query: 241 VPFGRLLNAGKAREAERLGEEIDGLFESVITARRE----GNNNQVQQDLLSLLL------ 300
            P  R L +   RE + L +E++ L   +I +RR+    G ++    DLL LLL      
Sbjct: 250 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDID 309

Query: 301 RDDGGRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNK 360
           +++     +   ++DECKTFFF GHETTAL +TWT +LL  +  WQ ++REEV+EV G  
Sbjct: 310 KNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRN 369

Query: 361 -----ENIGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMH 420
                + + K   +  V+ E LRLYP A  + R A +D+ +  LTIP G ++WI V+A+H
Sbjct: 370 GLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIH 429

Query: 421 HDEALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALI 480
           H E LWG   N+F+PERF     A G      ++PF  G R C+G+    ME KI++A +
Sbjct: 430 HSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILATL 489

Query: 481 LCRFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPL 497
           + +F+F++S  YRH+P ++L+++P +G+ +I +PL
Sbjct: 490 ISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of MS017770 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 3.5e-83
Identity = 173/507 (34.12%), Postives = 269/507 (53.06%), Query Frame = 0

Query: 6   LLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLESSQV- 65
           L ILL  +V    W+ P     KL+  G  GP PSF  GNI EM++  +        +  
Sbjct: 18  LCILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEAG 77

Query: 66  -----THNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGK 125
                + N  +T+FPYF  W   +G I++Y  G+   L + +P  +K L+   K+   GK
Sbjct: 78  STDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELA-NCKSLDLGK 137

Query: 126 PSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAH 185
           P   + +R ++ G G++ + GD WV QR VI P      +K M +LM+E+   ML  W +
Sbjct: 138 PCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKN 197

Query: 186 MI--NSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGV 245
            +    G  EI V+  +   + ++I++  FG     G+++F K+R LQ  + K + L+GV
Sbjct: 198 EVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGV 257

Query: 246 PFGRLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLR--DDGGRRLS 305
           P  R L     R    L   I  L  ++       ++  V +DLL  +++   DG     
Sbjct: 258 PGSRYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNKDLLHSIIQGSKDGPFASC 317

Query: 306 TTE--LVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNK----ENI 365
           T E  +VD CK  +F GHETT+    W L+LL  H EWQ++ R E  ++   +    + +
Sbjct: 318 TPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLDFDIL 377

Query: 366 GKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGD 425
            K  ++  V++E LRLYP A  V R+A  D+ + G+ IP GTN+WI +   H D ++WG 
Sbjct: 378 RKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPSVWGP 437

Query: 426 KVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSL 485
             ++F P+RF N  +AG C     Y+PFG G R C G++L  +E K++++L+L +F F L
Sbjct: 438 SADKFDPDRFAN-GIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKFEFKL 497

Query: 486 SPVYRHSPSIMLSLRPAHGLPLIFQPL 497
           SP Y H P+  L++ P  G+PLIF+ L
Sbjct: 498 SPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of MS017770 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.8e-80
Identity = 166/508 (32.68%), Postives = 271/508 (53.35%), Query Frame = 0

Query: 10  LVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKK-----KNKNCSVLESSQ- 69
           L +++    W+ P     K +    GGP PSFP GN+++M++     K    S   S   
Sbjct: 23  LAVYLYHILWLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAAAAAKAARASARRSGSG 82

Query: 70  ---VTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 129
              + H+    V PY+ +W+  +G IF Y +G   FL+++ P+ ++ ++  V     GK 
Sbjct: 83  GGGIVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCVSLDL-GKS 142

Query: 130 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 189
           S  K   + +FG G++ + G+ W+ QR +I P F    +K M  LMV+S   +L  W   
Sbjct: 143 SYLKATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEER 202

Query: 190 I--NSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVP 249
           +  N G  +I+++ +I A + ++I++T FG  +  G+++F K+R LQ  + K N L  + 
Sbjct: 203 VDRNGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAVSKPNVLAEMT 262

Query: 250 FGRLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTE 309
             R   + + ++A  L +++  L   ++    E  N      LLS +L      R+   E
Sbjct: 263 GLRFFPSMRNKQAWELHKQVRKLILEIVKESGEDRN------LLSAILHSASTSRVGIAE 322

Query: 310 ----LVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLG----NKENIG 369
               +VD CK+ +F GHE+TA+   W L+LLG+H EWQ ++REEV EV      +  ++ 
Sbjct: 323 AENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQ 382

Query: 370 KKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDK 429
           K   +  V++E LRLYP+   V RQA +++ + G+ IP G N++I V  MH D  LWG  
Sbjct: 383 KMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWGPD 442

Query: 430 VNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLS 489
           V EF+PERF +            YLPFG G R C+G+     E KI+++LI+ +F   LS
Sbjct: 443 VKEFNPERFSDVR-----PQLHSYLPFGAGARTCLGQGFAMAELKILISLIVSKFVLKLS 502

Query: 490 PVYRHSPSIMLSLRPAHGLPLIFQPLET 499
           P Y+HSP++ L + P  G+ L    +++
Sbjct: 503 PHYQHSPTLKLIVEPELGVDLTLTKVQS 518

BLAST of MS017770 vs. ExPASy Swiss-Prot
Match: B9G934 (Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 SV=2)

HSP 1 Score: 298.9 bits (764), Expect = 1.1e-79
Identity = 175/510 (34.31%), Postives = 276/510 (54.12%), Query Frame = 0

Query: 3   IMSLLILLVLWVLFCC---WISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVL 62
           I+ L+ILL L + + C   W+       KL+R G  GP P+F  GN  E+K+  +    L
Sbjct: 8   IVVLVILLSLALFYLCNILWLRAVKIRKKLRRQGIRGPKPTFLYGNTKEIKRIRQELK-L 67

Query: 63  ESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGK 122
              Q T+N  ST+FP+F  W+ ++G +F+Y  G    L ++ P+ +K +     ++  GK
Sbjct: 68  SQKQGTNNFISTLFPHFLLWRETYGPVFLYSTGAMEILQVSHPDMVKDIGRWTPSEL-GK 127

Query: 123 PSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAH 182
           P+  K  RK++FG GL    GD+W  QR +I P F    +K M  L+ ++T  +LE W  
Sbjct: 128 PNYLKKSRKALFGGGLFTENGDEWAYQRKIIAPEFFMDKIKGMIQLIEDATVPVLEAWED 187

Query: 183 MIN--SGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGV 242
           MI+   G REI V+  +   + ++IA+  FG     G ++F KLR LQ  + + +  VG+
Sbjct: 188 MIDDEGGCREIVVDDYLRNLSADVIARACFGSSFTKGEEIFCKLRQLQKAIARQDSFVGL 247

Query: 243 -PFGRLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDG---GRR 302
               + L    ++E + L E++  L   V  A+ + +       L++ ++  DG   GR 
Sbjct: 248 SALWKYLPTKSSQEIQMLDEQVRLLILDV--AKEQHHYQDSHNSLVNAII--DGAQDGRS 307

Query: 303 LSTTE--LVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKENIG- 362
            +  E  +V  CKT +FGGHE+TA+   W L+LL  H EWQ + R E  EV   +  +  
Sbjct: 308 AAEAEDFIVGNCKTIYFGGHESTAVTAIWCLMLLATHPEWQERARAEAMEVCRGRSTLDV 367

Query: 363 ----KKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEAL 422
               +   +  V++E LRLYP A  + R+A  D+ +  + +P GT + +  + +H D+  
Sbjct: 368 DALRRLKIVTMVIQETLRLYPPASVMMREALTDVKLGSIEVPRGTIVQVPRLMLHLDKEA 427

Query: 423 WGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFS 482
           WG   +EF P+RF N  VA  C     Y+PFG G R C+G++L   E K+++A +L +F+
Sbjct: 428 WGADADEFRPDRFAN-GVAAACRAAHMYVPFGHGPRTCIGQNLAMAELKVVLARLLTKFA 487

Query: 483 FSLSPVYRHSPSIMLSLRPAHGLPLIFQPL 497
           FS SP YRHSP+  L++ P  GLPL+   L
Sbjct: 488 FSPSPRYRHSPAFRLTIEPGFGLPLMVTKL 510

BLAST of MS017770 vs. ExPASy TrEMBL
Match: A0A6J1E0B2 (cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 2.7e-283
Identity = 488/504 (96.83%), Postives = 491/504 (97.42%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE
Sbjct: 8   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 67

Query: 61  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 120
           SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP
Sbjct: 68  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 127

Query: 121 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 180
           SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWA M
Sbjct: 128 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWARM 187

Query: 181 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFG 240
           INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVF+KLRALQMTLFKTNRLVGVPFG
Sbjct: 188 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFEKLRALQMTLFKTNRLVGVPFG 247

Query: 241 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELV 300
           RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELV
Sbjct: 248 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTELV 307

Query: 301 DECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKE------NIGKKMQ 360
           DECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKE      N+    +
Sbjct: 308 DECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKESEFDFSNLAGLKK 367

Query: 361 MGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEF 420
           MGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEF
Sbjct: 368 MGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEF 427

Query: 421 HPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPVYR 480
           HPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSP YR
Sbjct: 428 HPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPAYR 487

Query: 481 HSPSIMLSLRPAHGLPLIFQPLET 499
           HSPSIMLSLRPAHGLPLIFQ LET
Sbjct: 488 HSPSIMLSLRPAHGLPLIFQLLET 511

BLAST of MS017770 vs. ExPASy TrEMBL
Match: A0A6J1H0W5 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.9e-226
Identity = 400/514 (77.82%), Postives = 445/514 (86.58%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           L IM+ L  L+  +LF CWISPFLA+ KLKRNGF GPTPSFPLGNISEMK+ + +  V E
Sbjct: 9   LVIMAFLTSLLFKILFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFD-RVFE 68

Query: 61  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 120
           S  +TH+IHS VFPYFSRWQA+HGK+F YWLGTEPFLY+AEPEF+K LS EV+AK+WGKP
Sbjct: 69  S--LTHDIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKMLSREVQAKYWGKP 128

Query: 121 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 180
           SVFK DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVESTT+ML+RWAH+
Sbjct: 129 SVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHL 188

Query: 181 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFG 240
           + SG  +IEVE EIT+TAGEIIAKTSFGIDH SGRQVF KLR LQMTLFKTNRLVGVPF 
Sbjct: 189 VLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFA 248

Query: 241 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQV------QQDLLSLLLRDDG---- 300
            +LNAGKAREA+RLGEEID LF  VITARRE   NQ       Q DLLSLLL++ G    
Sbjct: 249 GILNAGKAREAKRLGEEIDELFREVITARRE---NQAAAEVVQQNDLLSLLLKESGGEGK 308

Query: 301 -GRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKEN- 360
            GR LSTTEL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQTQLR+E+KEVLG+KE+ 
Sbjct: 309 LGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLGDKESD 368

Query: 361 -----IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHD 420
                + +  +MGWVM EVLRLYPSAPNVQRQAR+DIT+NGLTIP+GTNMWIDVVAMHHD
Sbjct: 369 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHD 428

Query: 421 EALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILC 480
           EALWG++VNEF PERFE+D VAGGC+HKMGYLPFGFGGRMCVGRHLTFMEYKI++ LILC
Sbjct: 429 EALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILC 488

Query: 481 RFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           RFSF+LSP Y HSP IMLSLRPAHGLPLIFQ LE
Sbjct: 489 RFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 516

BLAST of MS017770 vs. ExPASy TrEMBL
Match: A0A6J1K6M8 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.9e-223
Identity = 394/514 (76.65%), Postives = 441/514 (85.80%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLE 60
           L IM+  I L+  VLF CWISPFLA+ KLKRNGF GPTPSFPLGNISEMK+ +    V E
Sbjct: 9   LVIMAFFISLLFKVLFSCWISPFLAHKKLKRNGFRGPTPSFPLGNISEMKRTSFG-RVFE 68

Query: 61  SSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKP 120
              +TH+IHS VFPYFSRWQA++GKIF YWLGTEPFLY+A+PEF+K LS EV+AK+WGKP
Sbjct: 69  C--LTHDIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKP 128

Query: 121 SVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHM 180
           SVFK DRKSMFGNGLVMAEGD+WVRQRHVITPAFNPSNLKAM SLMVESTT+ML+RWAH+
Sbjct: 129 SVFKRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHL 188

Query: 181 INSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFG 240
           + SG  +IEVE EIT+TAGEIIAKTSFGIDH SGRQVF KLR LQMTLFKTNRLVGVPF 
Sbjct: 189 VLSGHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFA 248

Query: 241 RLLNAGKAREAERLGEEIDGLFESVITARREGNNNQ------VQQDLLSLLLRDDGG--- 300
            +LNAGKAREA+RLGEEID LF  VITARRE  +        +Q DLLSLLL++ GG   
Sbjct: 249 GILNAGKAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKESGGEGK 308

Query: 301 --RRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKEN- 360
             R LST EL+DECKTFFFGGHETTALA+TWTLLLLGIHTEWQ QLR+E+KEVLG+KE+ 
Sbjct: 309 LRRWLSTAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLGDKESD 368

Query: 361 -----IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHD 420
                + +  +MGWVM EVLRLYPSAPNVQRQAR+DIT+NGLTIP+GTNMWIDVVAMHHD
Sbjct: 369 FDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHHD 428

Query: 421 EALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILC 480
           EALWG++VNEF PERFE+D VAGGC+HKMGYLPFGFGGRMCVGRHLTFMEYKI++ LILC
Sbjct: 429 EALWGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILC 488

Query: 481 RFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           RFSF+LSP Y HSP IMLSLRPAHGLPLIF+ LE
Sbjct: 489 RFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLE 519

BLAST of MS017770 vs. ExPASy TrEMBL
Match: A0A1S4DS94 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 6.0e-219
Identity = 388/528 (73.48%), Postives = 434/528 (82.20%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKK------NK 60
           L I  L IL VL VLFCCW+SPFL + KLKRNGF GPTP FPLGNISEMK K      N 
Sbjct: 11  LVIFGLFILFVLRVLFCCWVSPFLVHKKLKRNGFRGPTPCFPLGNISEMKMKMKMKNVNN 70

Query: 61  NCSVLESS------QVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTL 120
             S  E S       +THNIHSTVFPYF+RWQ ++GK+F YWLGTEPFLYIA+PEF+K L
Sbjct: 71  PNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIADPEFVKIL 130

Query: 121 STEVKAKFWGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVE 180
           S EVKAK WGKPSVFK+DRKSMFGNGLVMAEGD+WVRQRH+ITPAFNPSNLKAM SLMVE
Sbjct: 131 SKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVE 190

Query: 181 STTEMLERWAHMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTL 240
           S T+M+ERWA+++ SG+ +IEVE+EIT+TAGEIIAKTSFGI H SGRQVF+KLR LQ+TL
Sbjct: 191 SATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQITL 250

Query: 241 FKTNRLVGVPFGRLLNAGKAREAERLGEEIDGLFESVITARREG-----NNNQVQQDLLS 300
           FKTNRLVGVPF  LLNA KAREA+ LGEEID LF SVIT RRE         + Q DLLS
Sbjct: 251 FKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERTTTEQQNDLLS 310

Query: 301 LLLRD-------DGGRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLR 360
           LLL++       + GRRLST EL+DECKTFFFGGHETTALA+TWTL+LL +H+EWQT LR
Sbjct: 311 LLLKESSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILR 370

Query: 361 EEVKEVLGNK-------ENIGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPD 420
           EE+KEV G+         N+    +MGWVM EVLRLYPSAPNVQRQARKDI INGLTIP+
Sbjct: 371 EEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIIINGLTIPN 430

Query: 421 GTNMWIDVVAMHHDEALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHL 480
           GTNMWIDVV+MHHD+ALWG +VNEFHPERF ND V+GGCTHKMGYLPFGFGGRMCVGRHL
Sbjct: 431 GTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHL 490

Query: 481 TFMEYKIMVALILCRFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           TFMEYKI++ LIL RFSFSLSP YRHSPSIMLSLRPAHGLPL+F+ LE
Sbjct: 491 TFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLE 538

BLAST of MS017770 vs. ExPASy TrEMBL
Match: E5GCU7 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 1.0e-210
Identity = 372/506 (73.52%), Postives = 417/506 (82.41%), Query Frame = 0

Query: 23  FLAYNKLKRNGFGGPTPSFPLGNISEMKKK------NKNCSVLESS------QVTHNIHS 82
           F  + KLKRNGF GPTP FPLGNISEMK K      N   S  E S       +THNIHS
Sbjct: 6   FEVHKKLKRNGFRGPTPCFPLGNISEMKMKMKMKNVNNPNSTFEDSFSHDDQLITHNIHS 65

Query: 83  TVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPSVFKDDRKSM 142
           TVFPYF+RWQ ++GK+F YWLGTEPFLYIA+PEF+K LS EVKAK WGKPSVFK+DRKSM
Sbjct: 66  TVFPYFARWQTTYGKVFTYWLGTEPFLYIADPEFVKILSKEVKAKNWGKPSVFKNDRKSM 125

Query: 143 FGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHMINSGDREIEV 202
           FGNGLVMAEGD+WVRQRH+ITPAFNPSNLKAM SLMVES T+M+ERWA+++ SG+ +IEV
Sbjct: 126 FGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATKMVERWANLVISGNPQIEV 185

Query: 203 EREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFGRLLNAGKARE 262
           E+EIT+TAGEIIAKTSFGI H SGRQVF+KLR LQ+TLFKTNRLVGVPF  LLNA KARE
Sbjct: 186 EQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQITLFKTNRLVGVPFVGLLNAAKARE 245

Query: 263 AERLGEEIDGLFESVITARREG-----NNNQVQQDLLSLLLRD-------DGGRRLSTTE 322
           A+ LGEEID LF SVIT RRE         + Q DLLSLLL++       + GRRLST E
Sbjct: 246 AKLLGEEIDELFRSVITERREDAVAERTTTEQQNDLLSLLLKESSGGGGGEEGRRLSTAE 305

Query: 323 LVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNK-------ENIGK 382
           L+DECKTFFFGGHETTALA+TWTL+LL +H+EWQT LREE+KEV G+         N+  
Sbjct: 306 LIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIKEVFGHNLQTHFDFTNLSS 365

Query: 383 KMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKV 442
             +MGWVM EVLRLYPSAPNVQRQARKDI INGLTIP+GTNMWIDVV+MHHD+ALWG +V
Sbjct: 366 LKKMGWVMSEVLRLYPSAPNVQRQARKDIIINGLTIPNGTNMWIDVVSMHHDQALWGHQV 425

Query: 443 NEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSP 498
           NEFHPERF ND V+GGCTHKMGYLPFGFGGRMCVGRHLTFMEYKI++ LIL RFSFSLSP
Sbjct: 426 NEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSP 485

BLAST of MS017770 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 580.5 bits (1495), Expect = 1.3e-165
Identity = 286/503 (56.86%), Postives = 362/503 (71.97%), Query Frame = 0

Query: 6   LLILLVLWVLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLESSQ-- 65
           ++ L+ L +   CWI P  A  KL+ NGF GP PSFP GN+++MKK      V+++S+  
Sbjct: 20  VICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDNSKSS 79

Query: 66  --VTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPS 125
             + H+IHS   P+F+RWQ  +GK+F+YWLG EPF+Y+A+PEFL  +S  V  K WGKP+
Sbjct: 80  TIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSWGKPN 139

Query: 126 VFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHMI 185
           VFK DR+ MFG GLVM EGD W R RH+ITPAF P NLK M ++MVES + ML+RW   I
Sbjct: 140 VFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRWGIQI 199

Query: 186 NSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPFGR 245
           NSG+ E ++E EI  TAGEIIAKTSFG+   +G QV   LRA+Q  LF +NR VGVPF  
Sbjct: 200 NSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRYVGVPFSN 259

Query: 246 LLNAGKAREAERLGEEIDGLFESVITARR----EGNNNQVQQDLLSLLLRDDGGRRLSTT 305
           +L+  +  +A+ LG EIDGL  S I  R+    EG++     DLL +LL+ D     +  
Sbjct: 260 ILSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQ--GHDLLGMLLKADQKGNFTAK 319

Query: 306 ELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNKENIGKKM---- 365
           ELVDECKTFFF GHETTALA+TWT +LL IH EWQ  +REE++EV+G+ +    K+    
Sbjct: 320 ELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKIEYNKLAGLK 379

Query: 366 QMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNE 425
           +M WVM EVLRLYP APN QRQAR DI +NG  IP+GTN+WIDVVAMHHD  LWGD VNE
Sbjct: 380 KMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDVELWGDDVNE 439

Query: 426 FHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPVY 485
           F PERF+ + + GGC +KMGY+PFGFGGRMC+GR+LT MEYKI+++L+L RF  S+SP Y
Sbjct: 440 FKPERFDGN-LHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSRFEISVSPGY 499

Query: 486 RHSPSIMLSLRPAHGLPLIFQPL 497
           RHSP+ MLSLRP +GLPLI +PL
Sbjct: 500 RHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of MS017770 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 321.2 bits (822), Expect = 1.4e-87
Identity = 184/509 (36.15%), Postives = 279/509 (54.81%), Query Frame = 0

Query: 1   LAIMSLLILLVLWVLFCCW-ISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVL 60
           L I+  LIL VL+   CC+ ++P      ++R G  GP P    GNI ++ K   + +  
Sbjct: 11  LVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASN 70

Query: 61  ESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGK 120
           + S + HNI   + P++  W   +GK FI W GTEP L + E E +K L T+      GK
Sbjct: 71  DCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTK-HNPVTGK 130

Query: 121 PSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAH 180
             + +   K   G GL+MA G+ W  QRH+  PAF    LK     MVE T  M ER   
Sbjct: 131 SWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLRK 190

Query: 181 MINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVGVPF 240
            +     E+E+  E+     +II++T FG     G+++F  L  LQ    +  R +  P 
Sbjct: 191 EVG---EEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRHLCFPG 250

Query: 241 GRLLNAGKAREAERLGEEIDGLFESVITARRE----GNNNQVQQDLLSLLLR--DDGGRR 300
            R L +   RE + L  E++ L   +I +R++    G ++    DLL LLL   D     
Sbjct: 251 SRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKNN 310

Query: 301 LSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLG-----NKEN 360
           L+   ++DECKTFFF GHETT+L +TWTL+LL  +  WQ  +R+EV++V G     + E 
Sbjct: 311 LNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQ 370

Query: 361 IGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWG 420
           +     +  V+ E LRLYP A  + R A +DI +  L IP G ++WI V+A+HH   LWG
Sbjct: 371 LSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWG 430

Query: 421 DKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFS 480
           +  NEF+PERF   + A        ++PF  G R C+G+    ME KI++A+++ +FSF+
Sbjct: 431 EDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFA 490

Query: 481 LSPVYRHSPSIMLSLRPAHGLPLIFQPLE 498
           +S  YRH+P ++L+++P +G+ L+ +PL+
Sbjct: 491 ISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of MS017770 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 316.6 bits (810), Expect = 3.5e-86
Identity = 179/515 (34.76%), Postives = 280/515 (54.37%), Query Frame = 0

Query: 1   LAIMSLLILLVLW-VLFCCWISPFLAYNKLKRNGFGGPTPSFPLGNISEMKKKNKNCSVL 60
           L I    IL VL+  + C W++P      +++ G  GP P    GNI E+       +  
Sbjct: 10  LVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASK 69

Query: 61  ESSQVTHNIHSTVFPYFSRWQASHGKIFIYWLGTEPFLYIAEPEFLKTLSTE---VKAKF 120
           +   + H+I   + P++  W   +GK FI W GT+P L + E E +K L  +   V  + 
Sbjct: 70  DCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRS 129

Query: 121 WGKPSVFKDDRKSMFGNGLVMAEGDQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLER 180
           W    + +   K+  G GL+MA G  W  QRH+  PAF    LK     MVE T++++ER
Sbjct: 130 W----LQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVER 189

Query: 181 WAHMINSGDREIEVEREITATAGEIIAKTSFGIDHRSGRQVFDKLRALQMTLFKTNRLVG 240
               +  G  E+E+  E+     +II++T FG     G+++F+ L  LQ    +  R + 
Sbjct: 190 LRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQATRHLC 249

Query: 241 VPFGRLLNAGKAREAERLGEEIDGLFESVITARRE----GNNNQVQQDLLSLLL------ 300
            P  R L +   RE + L +E++ L   +I +RR+    G ++    DLL LLL      
Sbjct: 250 FPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDID 309

Query: 301 RDDGGRRLSTTELVDECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEVKEVLGNK 360
           +++     +   ++DECKTFFF GHETTAL +TWT +LL  +  WQ ++REEV+EV G  
Sbjct: 310 KNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRN 369

Query: 361 -----ENIGKKMQMGWVMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMH 420
                + + K   +  V+ E LRLYP A  + R A +D+ +  LTIP G ++WI V+A+H
Sbjct: 370 GLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIH 429

Query: 421 HDEALWGDKVNEFHPERFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALI 480
           H E LWG   N+F+PERF     A G      ++PF  G R C+G+    ME KI++A +
Sbjct: 430 HSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQFALMEAKIILATL 489

Query: 481 LCRFSFSLSPVYRHSPSIMLSLRPAHGLPLIFQPL 497
           + +F+F++S  YRH+P ++L+++P +G+ +I +PL
Sbjct: 490 ISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of MS017770 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-80
Identity = 168/492 (34.15%), Postives = 263/492 (53.46%), Query Frame = 0

Query: 28  KLKRNGFGGPTPSFPLGNISEMKKKNKNCSVLESSQ-------VTHNIHSTVFPYFSRWQ 87
           KL   G  GP PS   GN+ EM+K      ++ +S+       + H+  S++FPY   W+
Sbjct: 41  KLTMQGVKGPPPSLFRGNVPEMQKIQS--QIMSNSKHYSGDNIIAHDYTSSLFPYLDHWR 100

Query: 88  ASHGKIFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPSVFKDDRKSMFGNGLVMAEG 147
             +G+++ Y  G +  LY+  PE +K L+ +      GK S      KS+ G G++ + G
Sbjct: 101 KQYGRVYTYSTGVKQHLYMNHPELVKELN-QANTLNLGKVSYVTKRLKSILGRGVITSNG 160

Query: 148 DQWVRQRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHMI-NSGDR--EIEVEREITAT 207
             W  QR +I P F    +K M  L+VES   ML +W  M+   G+   +I V+ ++ A 
Sbjct: 161 PHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMKREGEMVCDIIVDEDLRAA 220

Query: 208 AGEIIAKTSFGIDHRSGRQVFDKLRALQMT------LFKTNRLVGVPFGRLLNA-GKARE 267
           + ++I++  FG     G+++F KLR LQ        LF  N    V FG   +  GK  E
Sbjct: 221 SADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNGFTDVVFGTKKHGNGKIDE 280

Query: 268 AERLGEEIDGLFESVITARREGNNNQVQQDLLSLLLRD-----DGGRRLSTTE----LVD 327
            ER    I+ L    +  R        ++DL+ L+L       DG     T      +VD
Sbjct: 281 LER---HIESLIWETVKERERECVGDHKKDLMQLILEGARSSCDGNLEDKTQSYKSFVVD 340

Query: 328 ECKTFFFGGHETTALAVTWTLLLLGIHTEWQTQLREEV----KEVLGNKENIGKKMQMGW 387
            CK+ +F GHET+A+AV+W L+LL ++  WQT++R+EV    K  + + ++I     +  
Sbjct: 341 NCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNGIPDADSISNLKTVTM 400

Query: 388 VMREVLRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEFHPE 447
           V++E LRLYP A  V R+A +D  +  L +P G  +W  +  +H D  +WG   NEF+PE
Sbjct: 401 VIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHRDPEIWGADANEFNPE 460

Query: 448 RFENDAVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPVYRHSP 490
           RF ++ V+  C H   ++PFG G R+C+G++   ME K++V+LI+ RFSF+LSP Y+HSP
Sbjct: 461 RF-SEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIVSRFSFTLSPTYQHSP 520

BLAST of MS017770 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 292.7 bits (748), Expect = 5.4e-79
Identity = 162/487 (33.26%), Postives = 262/487 (53.80%), Query Frame = 0

Query: 29  LKRNGFGGPTPSFPLGNISEMKK---KNKNCSVLESSQVTHNIHSTVFPYFSRWQASHGK 88
           LK  G  GP PS   GN+SEM++   + K+CS    + ++H+  S++FP+F  W+  +G+
Sbjct: 42  LKLQGVKGPPPSIFNGNVSEMQRIQSEAKHCS--GDNIISHDYSSSLFPHFDHWRKQYGR 101

Query: 89  IFIYWLGTEPFLYIAEPEFLKTLSTEVKAKFWGKPSVFKDDRKSMFGNGLVMAEGDQWVR 148
           I+ Y  G +  LYI  PE +K LS +      G+ +        + GNG++ + G  W  
Sbjct: 102 IYTYSTGLKQHLYINHPEMVKELS-QTNTLNLGRITHITKRLNPILGNGIITSNGPHWAH 161

Query: 149 QRHVITPAFNPSNLKAMGSLMVESTTEMLERWAHMINSGDR---EIEVEREITATAGEII 208
           QR +I   F    +K M  LMVES   ML +W  M+  G     +I V+ ++   + ++I
Sbjct: 162 QRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVI 221

Query: 209 AKTSFGIDHRSGRQVFDKLRAL------QMTLFKTNRLVGVPFGRLLNAGKAREAERLGE 268
           AK  FG     G+ +F  +R L      +  LF+ N    + FG   +     + + L  
Sbjct: 222 AKACFGSSFSKGKAIFSMIRDLLTAITKRSVLFRFNGFTDMVFGSKKHGDV--DIDALEM 281

Query: 269 EIDGLFESVITARREGNNNQVQQDLLSLLLRDDGGRRLSTTEL----------VDECKTF 328
           E++      +  R     +  ++DL+ L+L  +G  R     L          VD CK+ 
Sbjct: 282 ELESSIWETVKEREIECKDTHKKDLMQLIL--EGAMRSCDGNLWDKSAYRRFVVDNCKSI 341

Query: 329 FFGGHETTALAVTWTLLLLGIHTEWQTQLREEV----KEVLGNKENIGKKMQMGWVMREV 388
           +F GH++TA++V+W L+LL ++  WQ ++R+E+    K  + + E+I     +  V++E 
Sbjct: 342 YFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSSCKNGIPDAESIPNLKTVTMVIQET 401

Query: 389 LRLYPSAPNVQRQARKDITINGLTIPDGTNMWIDVVAMHHDEALWGDKVNEFHPERFEND 448
           +RLYP AP V R+A KDI +  L +P G  +W  + A+H D  +WG   N+F PERF ++
Sbjct: 402 MRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERF-SE 461

Query: 449 AVAGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIMVALILCRFSFSLSPVYRHSPSIMLS 490
            ++  C +   Y+PFG G R CVG++   ME K++V+LI+ +FSF+LSP Y+HSPS  L 
Sbjct: 462 GISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLL 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159617.15.5e-28396.83cytokinin hydroxylase-like [Momordica charantia][more]
XP_022958036.18.1e-22677.82cytokinin hydroxylase-like [Cucurbita moschata][more]
XP_023533013.13.7e-22377.15cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022995689.11.4e-22276.65cytokinin hydroxylase-like [Cucurbita maxima][more]
XP_038875746.19.5e-21974.85LOW QUALITY PROTEIN: cytokinin hydroxylase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9ZW952.0e-8636.15Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
Q9FF184.9e-8534.76Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q2QYH73.5e-8334.12Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... [more]
B6SSW84.8e-8032.68Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1[more]
B9G9341.1e-7934.31Cytochrome P450 714C3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C3 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1E0B22.7e-28396.83cytokinin hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025977 PE=3 S... [more]
A0A6J1H0W53.9e-22677.82cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV... [more]
A0A6J1K6M86.9e-22376.65cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1[more]
A0A1S4DS946.0e-21973.48cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1[more]
E5GCU71.0e-21073.52Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52400.11.3e-16556.86cytochrome P450, family 715, subfamily A, polypeptide 1 [more]
AT1G67110.11.4e-8736.15cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT5G38450.13.5e-8634.76cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT5G24910.11.3e-8034.15cytochrome P450, family 714, subfamily A, polypeptide 1 [more]
AT5G24900.15.4e-7933.26cytochrome P450, family 714, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 444..467
score: 29.44
coord: 318..344
score: 29.84
coord: 298..315
score: 28.15
coord: 434..444
score: 51.95
coord: 80..99
score: 22.64
IPR001128Cytochrome P450PRINTSPR00385P450coord: 357..368
score: 39.79
coord: 309..326
score: 35.67
coord: 435..444
score: 57.77
coord: 444..455
score: 34.73
IPR001128Cytochrome P450PFAMPF00067p450coord: 69..472
e-value: 9.8E-75
score: 252.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 22..497
e-value: 1.2E-106
score: 359.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 29..495
NoneNo IPR availablePANTHERPTHR24282:SF149CYTOCHROME P450 FAMILY 72 PROTEINcoord: 4..492
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 4..492
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 437..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017770.1MS017770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen