MS017750 (gene) Bitter gourd (TR) v1

Overview


Name	MS017750
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold373: 2151906 .. 2155501 (-)
RNA-Seq Expression	MS017750
Synteny	MS017750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTGGGCAGGTTTCGGCGGCCGTCATTCTATTGGCATTGGTGAGTTTAGCAACCGTCGATGGCAGAAAACCGTGTCCAAATTCTAACTGCGAGGAGTTTCCGGCGATTAATTGCCGGAAGTACTCGGCGGTGCTGACGGAGTTCGGCGCCGTCGGCGACGGAGTTACTTCAAATACGCACGCGTTCCGGCAAGCGATCGAGCATCTGAAGCCGCTGGCGGCGGCCGGCGGCGCTCAGCTGTTTGTTCCGGCGGGGAAATGGCTCACCGGAAGCTTTAATCTCACCAGCCATTTCACTCTCTTCCTCCATAAGGATGCTCAAATTCTAGCTTCTCAGGTTTCCCCTTCATCTTTATATATATTGAAATGGAAATATTTATATATTTTTAAGTCATTTTAATTTCACCTTTTTTAATTTGTCACAAGATATCGATTTTTTTACCCATTATTCAAAATTTCGAATTTTCATCCCATGTGTTGAACTAAAAAAAGAGGTAAAACAGTTCAAAACGAATCACATGGATATCCACTTCATTATTTTGGAAAATTTTTATTTAGTATCACCTAAAAAAAATAAAAGTGGGAAAAAAATAGCACATGGATACCCTTATTCCTTTGGTGTAATTAAAGAATAAAAAGAGGATAAAGAAAAGTACATGGAATGAAGTTTGAAATGTTTTAATAGTATGAACTAAAAAGGGTGATTTGGAAAGAATTAAAAAGTTGGAGGGTGTATTTGAGAATTTGTAATAAATTTGTTGCATAATGAAATATTAATTTGTAGTCTTCGGTTTGATGTGGGGGTTTTTTTTGGGTATTATATATAGGCTATCTCTTTTTTAATTAGGTAGAAGTGCTTAAAAAGTGACATTAATTATTTTTCTGAAATGGAAATAAATGTTTATTTAGTGAGTGATATATTAAATATGGTGAGGTAGATATTGACTTCATTGATAGAGTCAAAATTAGTTCAATTTGGAAGAGAGAGAAAAAAAACTGCGTTTGGAATTTTAATTCTGATTTATTCCGACCCGACCCGACCCAATGGGGTACTGATCGGCTGCGGGCGAGAGTATTGAGAACTGATTGGAATTGGAAAATCGGACGGTGCAGGATGAATCAGAATGGCCACTCGTGGCAATTCTTCCTTCTTATGGAAGGGGAAGAGACGCGCCTGGTGGACGCTTCAGCAGTCTCATTTATGGAACCAATCTCACCGATGTTGTCATCACAGGTGATTACTTCTCTCTATTTTTCTTTTTCTATTTCTCTAAATTTAAAAATTCGTTCACCAAACCATTTTTTACGACCTAACTTGCGAAAATTAAAAAACAAAAAATGGCAGTTTTCAAAAAGAGTTTTTTTTTCTTTTTATTTTTAAAGTTTGACTAGAACTAATGTATAGCATGCAAACTTAATCTTAAAATTTTGAAAATTAGAAACTTTGTGAATTTGTTGACCTCCTCCTCCTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTTTACTTGGTTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTAAAAATTTATGCATTTTTTTCGCAATTTTAAAATAAACAAAAAAGAAGTAAATAAATATATATTTTAAAAGCATAAAACCAAGCCTGGGTTGAGTTAAATACCTTGAAATGTTATGCATTCGTTTGTTTTGTTATATAGTTTTTAAAAATATTTTCAAAATCTAAATAAAACAATTCAAATGCCTTTTCAAAAAGGTGAAAAAATAACCAATAAAATTGTAAAAAAACATGTAAAAAATTTTAAAAATTTGAAACTAAAAATGAAAGGGCAATTGATAATGAAATTTATAAAAGAGGTAAAAAAGTGGGGCCCACTCATTGTATTGGTGACGTGGTGTAGGGAACAACGGGACGATTGACGGGCAGGGATCTTATTGGTGGGATAAATTCCACAAAAACGAGCTCAACGTAACCCGGCCGTACCTGATCGAGATCATGTACTCCGATCAGATCCAGATCTCTAATCTCACGTTGGTCAACTCTCCTTCTTGGTTCGTCCATCCGATCTACAGCAGGTTTGACTTTTTGCCTTCATCGCACGGCTCCTATTCCTTCCCATTTATTTATTTTATTTTATTTTATTTTATCATGTTCTGTTCATTACATTGTTTCCAGCTCTATACTCTCACATCATTAATCTTGTTTCAATAAGATTTCATCAGTTTATTTAAAAGTAGAATACTAAAATTTTGAAAAATAGTATTCTTTTTTAATTTTTTTTAATTCATTTTGATCTATTTCGTTCAATTTTGTTGGTTCAAAGGGTTTGGTCAATTTCTAAGTTTGGATTGAGGTTATAGGTTCAAAATCCAGATTAGTTTTTTTCTTTACCAACACTAATACTTTTGAAAATAAATTTGTAATTAACATTAACTATTTTTTTAAAGAACTAATTTGAGGTATACAAAAAAGAAAAATATGATACTTTTTTTATGGTATTTAGTATGAAATAAACACAATGTAATGTAAAATATTTAGGTCCTGCCTTTAATAAGTGCTGACTATATATTATGTTCAAAGTTTCAATAGTGAATTTTCCTATCCTATATTCTAAAAAAAATTGTAAAATTAATATTGATTTTATGCAAGTGTAATTTCTTAAGAAAAAGAGTAAAAAGAAAATGTGTGTTTTTTTTTAATGAATTAATTTTGTGGTGGTGAAAATGCAGTAATGTGATAATTCAAGGGCTCACAATTCTGGCTCCAATCGACTCTCCCAACACGGACGGAATAGACCCAGGTTCATTCAATTTCCAAAATTAGGGCTTAGGGTTAAGGTTTGTTTTTTCTTCTTCTTCCTCAATCCTCATCCCTAATTTCTCGATCCAATTCCAGATTCCTGCACCAACACACGAATCGAGGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAATACGGGATTCGATTCGGGATGCCGACCGAGCACCTCCTCATCCGCCGCCTCACCTGCATCTCCCCTGACAGCGCCGGAATCGCCCTCGGCAGCGAAATGTCCGGCGGAATCCGCGACGTCAGGATCGAGGACGTCACCGCCATCAACACTCAGTCCGCCGTCCGGATCAAGACCGCGCGTGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGCCGGATGTTCCTCTCCACCATGAAGTACGTCTTCTGGATGACCGGAGACTACAAATCGCATCCGGATCCCGAGTTCGACCCGGCCGCCATTCCCGAGATTAAGAACATCAATTACAGAGACGTGGTGGCAGAGAACGTGACGTACGCCGCCAGATTGGAGGGCATCTCCGGAGATCCCTTCACCGACATCTGTGTTACCAACGTCACCATCGGCCTCACCAAGAAGCCGAAGAAGTTGCAGTGGAACTGTACGAATGTTGAGGGATTCAGTAGCGGTGTGGTGCCGCCGCCGTGCGCTCCGCTCGCCAAGCCGGCGGAAGGCGGCGGCTGTGCTTTCCCGACAGATCGGCTGCCGATCGAGGAGTTTCCGTTGAAGACCTGCAGTATTTCAACTTGT

mRNA sequence

TTTTTTGGGCAGGTTTCGGCGGCCGTCATTCTATTGGCATTGGTGAGTTTAGCAACCGTCGATGGCAGAAAACCGTGTCCAAATTCTAACTGCGAGGAGTTTCCGGCGATTAATTGCCGGAAGTACTCGGCGGTGCTGACGGAGTTCGGCGCCGTCGGCGACGGAGTTACTTCAAATACGCACGCGTTCCGGCAAGCGATCGAGCATCTGAAGCCGCTGGCGGCGGCCGGCGGCGCTCAGCTGTTTGTTCCGGCGGGGAAATGGCTCACCGGAAGCTTTAATCTCACCAGCCATTTCACTCTCTTCCTCCATAAGGATGCTCAAATTCTAGCTTCTCAGGATGAATCAGAATGGCCACTCGTGGCAATTCTTCCTTCTTATGGAAGGGGAAGAGACGCGCCTGGTGGACGCTTCAGCAGTCTCATTTATGGAACCAATCTCACCGATGTTGTCATCACAGGGAACAACGGGACGATTGACGGGCAGGGATCTTATTGGTGGGATAAATTCCACAAAAACGAGCTCAACGTAACCCGGCCGTACCTGATCGAGATCATGTACTCCGATCAGATCCAGATCTCTAATCTCACGTTGGTCAACTCTCCTTCTTGGTTCGTCCATCCGATCTACAGCAGTAATGTGATAATTCAAGGGCTCACAATTCTGGCTCCAATCGACTCTCCCAACACGGACGGAATAGACCCAGATTCCTGCACCAACACACGAATCGAGGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAATACGGGATTCGATTCGGGATGCCGACCGAGCACCTCCTCATCCGCCGCCTCACCTGCATCTCCCCTGACAGCGCCGGAATCGCCCTCGGCAGCGAAATGTCCGGCGGAATCCGCGACGTCAGGATCGAGGACGTCACCGCCATCAACACTCAGTCCGCCGTCCGGATCAAGACCGCGCGTGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGCCGGATGTTCCTCTCCACCATGAAGTACGTCTTCTGGATGACCGGAGACTACAAATCGCATCCGGATCCCGAGTTCGACCCGGCCGCCATTCCCGAGATTAAGAACATCAATTACAGAGACGTGGTGGCAGAGAACGTGACGTACGCCGCCAGATTGGAGGGCATCTCCGGAGATCCCTTCACCGACATCTGTGTTACCAACGTCACCATCGGCCTCACCAAGAAGCCGAAGAAGTTGCAGTGGAACTGTACGAATGTTGAGGGATTCAGTAGCGGTGTGGTGCCGCCGCCGTGCGCTCCGCTCGCCAAGCCGGCGGAAGGCGGCGGCTGTGCTTTCCCGACAGATCGGCTGCCGATCGAGGAGTTTCCGTTGAAGACCTGCAGTATTTCAACTTGT

Coding sequence (CDS)

TTTTTTGGGCAGGTTTCGGCGGCCGTCATTCTATTGGCATTGGTGAGTTTAGCAACCGTCGATGGCAGAAAACCGTGTCCAAATTCTAACTGCGAGGAGTTTCCGGCGATTAATTGCCGGAAGTACTCGGCGGTGCTGACGGAGTTCGGCGCCGTCGGCGACGGAGTTACTTCAAATACGCACGCGTTCCGGCAAGCGATCGAGCATCTGAAGCCGCTGGCGGCGGCCGGCGGCGCTCAGCTGTTTGTTCCGGCGGGGAAATGGCTCACCGGAAGCTTTAATCTCACCAGCCATTTCACTCTCTTCCTCCATAAGGATGCTCAAATTCTAGCTTCTCAGGATGAATCAGAATGGCCACTCGTGGCAATTCTTCCTTCTTATGGAAGGGGAAGAGACGCGCCTGGTGGACGCTTCAGCAGTCTCATTTATGGAACCAATCTCACCGATGTTGTCATCACAGGGAACAACGGGACGATTGACGGGCAGGGATCTTATTGGTGGGATAAATTCCACAAAAACGAGCTCAACGTAACCCGGCCGTACCTGATCGAGATCATGTACTCCGATCAGATCCAGATCTCTAATCTCACGTTGGTCAACTCTCCTTCTTGGTTCGTCCATCCGATCTACAGCAGTAATGTGATAATTCAAGGGCTCACAATTCTGGCTCCAATCGACTCTCCCAACACGGACGGAATAGACCCAGATTCCTGCACCAACACACGAATCGAGGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAATACGGGATTCGATTCGGGATGCCGACCGAGCACCTCCTCATCCGCCGCCTCACCTGCATCTCCCCTGACAGCGCCGGAATCGCCCTCGGCAGCGAAATGTCCGGCGGAATCCGCGACGTCAGGATCGAGGACGTCACCGCCATCAACACTCAGTCCGCCGTCCGGATCAAGACCGCGCGTGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGCCGGATGTTCCTCTCCACCATGAAGTACGTCTTCTGGATGACCGGAGACTACAAATCGCATCCGGATCCCGAGTTCGACCCGGCCGCCATTCCCGAGATTAAGAACATCAATTACAGAGACGTGGTGGCAGAGAACGTGACGTACGCCGCCAGATTGGAGGGCATCTCCGGAGATCCCTTCACCGACATCTGTGTTACCAACGTCACCATCGGCCTCACCAAGAAGCCGAAGAAGTTGCAGTGGAACTGTACGAATGTTGAGGGATTCAGTAGCGGTGTGGTGCCGCCGCCGTGCGCTCCGCTCGCCAAGCCGGCGGAAGGCGGCGGCTGTGCTTTCCCGACAGATCGGCTGCCGATCGAGGAGTTTCCGTTGAAGACCTGCAGTATTTCAACTTGT

Protein sequence

FFGQVSAAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAKPAEGGGCAFPTDRLPIEEFPLKTCSISTC

Homology

[Close]

BLAST of MS017750 vs. NCBI nr
Match: XP_038906749.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 834.7 bits (2155), Expect = 3.9e-238
Identity = 395/458 (86.24%), Postives = 429/458 (93.67%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVI+LALVSLATV+G+KPCP +N  E PAI+CRK++AVLT+FG VGDGVTSNTHAFRQA
Sbjct: 18  SAVIILALVSLATVEGKKPCPTTNYLEVPAISCRKHTAVLTDFGGVGDGVTSNTHAFRQA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HK A ILASQDESEWPLVA+LPS
Sbjct: 78  IEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKYATILASQDESEWPLVAVLPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YGRGRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHK+ELNVTRPYLIE+M
Sbjct: 138 YGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKDELNVTRPYLIEMM 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQIS+LTLVNSPSWFVHPIYS NVII+GLTILAPIDSPNTDGIDPDSC+NTRIEDC
Sbjct: 198 YSDQIQISDLTLVNSPSWFVHPIYSRNVIIRGLTILAPIDSPNTDGIDPDSCSNTRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPTAVP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EI NINYRDVVAENVTY+ARLEGISGDPFTDIC++NV IGLT KPKKLQWNCT+VEGFSS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSS 437

Query: 427 GVVPPPCAPLAKPAEGGGCAFPTDRLPIEEFPLKTCSI 465
            V+PPPCAPLAK A+ GGC FP D LPIE+  LKTC +
Sbjct: 438 DVMPPPCAPLAKAAKSGGCYFPEDELPIEKVQLKTCFV 475

[Close]

BLAST of MS017750 vs. NCBI nr
Match: XP_023532553.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 825.9 bits (2132), Expect = 1.8e-235
Identity = 394/457 (86.21%), Postives = 424/457 (92.78%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILLA VSL +VDG+KPCP S+  EFPAI+CRK++AVLT+FG VGDGVTSNT+AFR+A
Sbjct: 18  SAVILLAFVSLGSVDGKKPCPFSSYLEFPAISCRKHTAVLTDFGGVGDGVTSNTNAFREA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           +EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWPLV +LPS
Sbjct: 78  MEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWPLVEVLPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFH+NELNVTRPY+IEIM
Sbjct: 138 YGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHRNELNVTRPYMIEIM 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC NTRIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS
Sbjct: 378 EIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSS 437

Query: 427 GVVPPPCAPLAKP-AEGGGCAFPTDRLPIEEFPLKTC 463
            VVPPPCAPLAK      GC FP DRLPIE+  L TC
Sbjct: 438 DVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDVKLMTC 474

[Close]

BLAST of MS017750 vs. NCBI nr
Match: XP_008437531.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK05998.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 819.3 bits (2115), Expect = 1.7e-233
Identity = 391/458 (85.37%), Postives = 425/458 (92.79%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDFGGVGDGVTSNTQAFRRA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA ILASQDESEWP VAILPS
Sbjct: 78  IEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATILASQDESEWPQVAILPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHK ELN+TRPY+IEI+
Sbjct: 138 YGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKGELNLTRPYMIEIL 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+N RIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNIRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISS 437

Query: 427 GVVPPPCAPLAKPAEGGGCAFPTDRLPIEEFPLKTCSI 465
            VVPPPCAPLAK A+ GGC FP D+LPIE+  LKTC +
Sbjct: 438 DVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFV 474

[Close]

BLAST of MS017750 vs. NCBI nr
Match: XP_022996001.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 818.1 bits (2112), Expect = 3.7e-233
Identity = 392/457 (85.78%), Postives = 422/457 (92.34%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILL LVSLA+VDG+KPCP S+  EF AI+CRK++AVLT+FG VGDGVTSNT+AFR+A
Sbjct: 18  SAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDFGGVGDGVTSNTNAFREA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           IEHL  LA  GG+QL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWPLV +LPS
Sbjct: 78  IEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWPLVEVLPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTRPY+IEIM
Sbjct: 138 YGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTRPYMIEIM 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC NTRIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS
Sbjct: 378 EIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSS 437

Query: 427 GVVPPPCAPLAKP-AEGGGCAFPTDRLPIEEFPLKTC 463
            VVP PCAPLAK      GC FP D+LPIE+  L TC
Sbjct: 438 DVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTC 474

[Close]

BLAST of MS017750 vs. NCBI nr
Match: XP_022957618.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 392/464 (84.48%), Postives = 424/464 (91.38%), Query Frame = 0


Query: 4   QVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSN 63
           Q++AA    VILLALVSL +VDG+K CP S+  EFPAI+CRK++AVLT+FG +GDGVTSN
Sbjct: 11  QITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDFGGIGDGVTSN 70

Query: 64  THAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWP 123
           T+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWP
Sbjct: 71  TNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWP 130

Query: 124 LVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTR 183
           LV +LPSYGRGRDAP GRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTR
Sbjct: 131 LVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTR 190

Query: 184 PYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCT 243
           PY+IEIMYS+QIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC 
Sbjct: 191 PYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCI 250

Query: 244 NTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGG 303
           NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGG
Sbjct: 251 NTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGG 310

Query: 304 IRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPE 363
           IR+VRIE+VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTGDYKSHPD +
Sbjct: 311 IRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGDYKSHPDEK 370

Query: 364 FDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCT 423
           FDP A+PEIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++N+ IGLT K KKLQWNCT
Sbjct: 371 FDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTAKQKKLQWNCT 430

Query: 424 NVEGFSSGVVPPPCAPLAKP-AEGGGCAFPTDRLPIEEFPLKTC 463
           NVEG+SS VVPPPCAPLAK      GC FP DRLPIE+  L TC
Sbjct: 431 NVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTC 474

[Close]

BLAST of MS017750 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.2e-179
Identity = 299/464 (64.44%), Postives = 363/464 (78.23%), Query Frame = 0


Query: 2   FGQVSAAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTH 61
           F  VS  V ++ L+S++  + RK        E+ AI+CR +SA L +FG VGDG T NT 
Sbjct: 22  FQVVSVLVAVVLLLSVSRGECRKG-RILEALEYSAISCRAHSASLVDFGGVGDGQTLNTK 81

Query: 62  AFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLV 121
           AF+ A+  L    + GGAQL+VPAGKWLTGSF+LTSHFTLFLH+DA +LASQD S+WP++
Sbjct: 82  AFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVI 141

Query: 122 AILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPY 181
             LPSYGRGRDA  GR++SLI+GTNLTDV+ITG+NGTIDGQG  WW +FH  +L  TRPY
Sbjct: 142 KPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPY 201

Query: 182 LIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNT 241
           LIE+MYS  IQISNLTL+NSPSW VHP+YS N++IQG+TILAP+ SPNTDGI+PDSCTNT
Sbjct: 202 LIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNT 261

Query: 242 RIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIR 301
           RIEDC+IVSGDDC+AVKSGWD+YGI +GMPT+ L+IRRLTCISP SA IALGSEMSGGI+
Sbjct: 262 RIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQ 321

Query: 302 DVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFD 361
           DVR ED+ AIN++S +RIKT  GRGG+VKDI+VR M + TMK+ FWMTG+Y SH D  +D
Sbjct: 322 DVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYD 381

Query: 362 PAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNV 421
           P A P I+ INYRD+VAENV+ AARLEGI  DPFT IC++NVTI L  K KK+ W CT+V
Sbjct: 382 PKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDV 441

Query: 422 EGFSSGVVPPPCAPLAK--PAEGGGCAFPTDRLPIEEFPLKTCS 464
           EG SSGV P PC+ L    P +   C FP + LPI+   L+ CS
Sbjct: 442 EGISSGVTPTPCSTLPDQGPEKTSLCNFPAESLPIDTVELQKCS 484

[Close]

BLAST of MS017750 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.3e-30
Identity = 116/403 (28.78%), Postives = 179/403 (44.42%), Query Frame = 0


Query: 46  LTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKW-------LTGSFNLTSH 105
           +T+FGAVGDGVT ++ AF +A E +   +  G  Q  VPAG           GS   T  
Sbjct: 26  VTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTFMLQPLKFQGSCKSTPV 85

Query: 106 FTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGT 165
           F   L K                 + PS G  +    G     I  T++  +VI G +G 
Sbjct: 86  FVQMLGK----------------LVAPSKGNWK----GDKDQWILFTDIEGLVIEG-DGE 145

Query: 166 IDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQG 225
           I+GQGS WW+  HK     +RP  ++    + +++S LT ++SP   +H    + V I  
Sbjct: 146 INGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISS 205

Query: 226 LTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIR 285
           L I AP  SPNTDGID  + +N  I+DC I +GDDCIA+ SG           T ++ I 
Sbjct: 206 LRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHIS 265

Query: 286 RLTCISPDSAGIALGSEMSGG----IRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFV 345
            + C      GI++GS    G    + +V +++     T +  RIKT +G  G+ + I  
Sbjct: 266 GIDC--GPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITF 325

Query: 346 RRMFLSTMKY-----VFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEG 405
             + L  ++       F+  GD  +  D +     + ++   N+  +      Y      
Sbjct: 326 NGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNF--IGTSKSEYGVDFRC 384

Query: 406 ISGDPFTDICVTNVTI--GLTKKPKKLQWNCTNVEGFSSGVVP 431
               P T+I + ++ I    +   +  Q  C NV G S+  VP
Sbjct: 386 SERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVP 384

[Close]

BLAST of MS017750 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-28
Identity = 121/408 (29.66%), Postives = 174/408 (42.65%), Query Frame = 0


Query: 48  EFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS---FNLTSHFTLFLH 107
           ++GAVGDG   +T AF               A L VPA K    +   F       L   
Sbjct: 63  QYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLSFK 122

Query: 108 KDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGS 167
            D  I+A  D + W    I   + +                 LTD  + G  G IDGQG 
Sbjct: 123 VDGTIVAQPDPARWKNSKIWLQFAQ-----------------LTDFNLMG-TGVIDGQGQ 182

Query: 168 YWWDKFHK--------NELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVII 227
            WW    K        N+ N  RP  I+I YS  + +  LTL+NSP + +       V I
Sbjct: 183 QWWAGQCKVVNGRTVCNDRN--RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKI 242

Query: 228 QGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLL 287
           QGL I AP DSPNTDGID  +     IE C I +GDDCIA+ +G           + ++ 
Sbjct: 243 QGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------SSNIT 302

Query: 288 IRRLTCISPDSAGIALGS----EMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 347
           I+ L C      GI++GS         +  V +     I+TQ+ +RIKT +G  G    I
Sbjct: 303 IKDLIC--GPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYI 362

Query: 348 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAA-RLEGIS 407
               + +   +    +   Y +      +  +  +I+ + Y+++   + T AA +L    
Sbjct: 363 TYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSD 422

Query: 408 GDPFTDICVTNVTIGLTK-KPKKLQWNCT--NVEGFSSGVVPPPCAPL 437
             P T I ++NV++ LT  KP     +C   N  GF SG + P C  L
Sbjct: 423 SVPCTGIQLSNVSLKLTSGKPA----SCVDKNARGFYSGRLIPTCKNL 430

[Close]

BLAST of MS017750 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.5e-27
Identity = 117/407 (28.75%), Postives = 177/407 (43.49%), Query Frame = 0


Query: 49  FGAVGDGVTSNTHAFRQAIEHLKPLAAAG--GAQLFVPAGKWLTGSFNLT------SHFT 108
           +GAVGDG   +T AF +        AA     A   VPA K    + NL        HF+
Sbjct: 65  YGAVGDGKHDSTDAFEKTWN-----AACNKLSAVFLVPANKKFVVN-NLVFYGPCQPHFS 124

Query: 109 LFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTID 168
             +  D  I A  D ++W    I   + R                 LTD  + G  G ID
Sbjct: 125 FKV--DGTIAAYPDPAKWKNSKIWMHFAR-----------------LTDFNLMG-TGVID 184

Query: 169 GQGSYWWDKFHKNELNVT------RPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNV 228
           GQG+ WW    K     T      RP  I+I +S  + +  LTL NSP + +       V
Sbjct: 185 GQGNRWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGV 244

Query: 229 IIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEH 288
            IQG+ I AP DSPNTDGID  +     IE C I +GDDC+AV +G           + +
Sbjct: 245 KIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------SSN 304

Query: 289 LLIRRLTCISPDSAGIALGSEMSGGIRD----VRIEDVTAINTQSAVRIKTARGRGGFVK 348
           + I+ LTC      G+++GS   G  R     V ++    I+TQ+ +RIKT +G  G   
Sbjct: 305 ITIKDLTC--GPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLAS 364

Query: 349 DIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAA-RLEG 408
            I    + +   +    +   Y +      +  +  +I+++ ++++   + T AA +L  
Sbjct: 365 HITYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMC 424

Query: 409 ISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPL 437
               P ++I ++NV + LT        N  N  G+ +  + P C  L
Sbjct: 425 SDSVPCSNIKLSNVFLKLTSGKVATCVN-KNANGYYTNPLNPSCKSL 431

[Close]

BLAST of MS017750 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 2.9e-26
Identity = 106/399 (26.57%), Postives = 174/399 (43.61%), Query Frame = 0


Query: 46  LTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGK-WLTGSFNLTS--HFTLF 105
           +++FGA GDG T +T AF  A +  K  ++ G   L VP G  +L  S  LT   +  L 
Sbjct: 70  VSDFGAKGDGKTDDTQAFVNAWK--KACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 129

Query: 106 LHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQ 165
           +     + ASQ  S++  ++              ++       NL+  V  G+ G +DG 
Sbjct: 130 VQIFGTLSASQKRSDYKDIS--------------KWIMFDGVNNLS--VDGGDTGVVDGN 189

Query: 166 GSYWWD---KFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGL 225
           G  WW    K +K +     P  +    S  + + NL + N+    +     SNV +  +
Sbjct: 190 GETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNV 249

Query: 226 TILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRR 285
            + AP DSPNTDGI   +  N R+ +  I +GDDCI+++SG           ++++ I  
Sbjct: 250 VVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQNVQIND 309

Query: 286 LTCISPDSAGIALGS----EMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVR 345
           +TC      GI++GS         +  V ++      T + VRIKT +G  G   +I  +
Sbjct: 310 ITC--GPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQ 369

Query: 346 RMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISGD-P 405
            + +  +K    +  DY        + +A+ ++KN+ YRD+   + +  A     S + P
Sbjct: 370 NIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTSASENAITFNCSKNYP 429

Query: 406 FTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPC 434
              I +  V I      K  +  CTN      G V P C
Sbjct: 430 CQGIVLDRVNI------KGGKATCTNANVVDKGAVLPQC 430

[Close]

BLAST of MS017750 vs. ExPASy TrEMBL
Match: A0A5D3C461 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00950 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 8.1e-234
Identity = 391/458 (85.37%), Postives = 425/458 (92.79%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDFGGVGDGVTSNTQAFRRA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA ILASQDESEWP VAILPS
Sbjct: 78  IEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATILASQDESEWPQVAILPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHK ELN+TRPY+IEI+
Sbjct: 138 YGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKGELNLTRPYMIEIL 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+N RIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNIRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISS 437

Query: 427 GVVPPPCAPLAKPAEGGGCAFPTDRLPIEEFPLKTCSI 465
            VVPPPCAPLAK A+ GGC FP D+LPIE+  LKTC +
Sbjct: 438 DVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFV 474

[Close]

BLAST of MS017750 vs. ExPASy TrEMBL
Match: A0A1S3AUV5 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 8.1e-234
Identity = 391/458 (85.37%), Postives = 425/458 (92.79%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDFGGVGDGVTSNTQAFRRA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           IEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA ILASQDESEWP VAILPS
Sbjct: 78  IEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATILASQDESEWPQVAILPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHK ELN+TRPY+IEI+
Sbjct: 138 YGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKGELNLTRPYMIEIL 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+N RIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNIRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VT INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+P
Sbjct: 318 NVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS
Sbjct: 378 EITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISS 437

Query: 427 GVVPPPCAPLAKPAEGGGCAFPTDRLPIEEFPLKTCSI 465
            VVPPPCAPLAK A+ GGC FP D+LPIE+  LKTC +
Sbjct: 438 DVVPPPCAPLAKAAKSGGCDFPADQLPIEKVQLKTCFV 474

[Close]

BLAST of MS017750 vs. ExPASy TrEMBL
Match: A0A6J1K3G8 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.8e-233
Identity = 392/457 (85.78%), Postives = 422/457 (92.34%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILL LVSLA+VDG+KPCP S+  EF AI+CRK++AVLT+FG VGDGVTSNT+AFR+A
Sbjct: 18  SAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDFGGVGDGVTSNTNAFREA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPS 126
           IEHL  LA  GG+QL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWPLV +LPS
Sbjct: 78  IEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWPLVEVLPS 137

Query: 127 YGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIM 186
           YGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTRPY+IEIM
Sbjct: 138 YGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTRPYMIEIM 197

Query: 187 YSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDC 246
           YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC NTRIEDC
Sbjct: 198 YSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDC 257

Query: 247 FIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIE 306
           FIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE
Sbjct: 258 FIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIE 317

Query: 307 DVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIP 366
           +VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+P
Sbjct: 318 NVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALP 377

Query: 367 EIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSS 426
           EIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS
Sbjct: 378 EIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSS 437

Query: 427 GVVPPPCAPLAKP-AEGGGCAFPTDRLPIEEFPLKTC 463
            VVP PCAPLAK      GC FP D+LPIE+  L TC
Sbjct: 438 DVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTC 474

[Close]

BLAST of MS017750 vs. ExPASy TrEMBL
Match: A0A6J1H0R5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.3e-233
Identity = 392/464 (84.48%), Postives = 424/464 (91.38%), Query Frame = 0


Query: 4   QVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSN 63
           Q++AA    VILLALVSL +VDG+K CP S+  EFPAI+CRK++AVLT+FG +GDGVTSN
Sbjct: 11  QITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDFGGIGDGVTSN 70

Query: 64  THAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWP 123
           T+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQILASQDESEWP
Sbjct: 71  TNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQILASQDESEWP 130

Query: 124 LVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTR 183
           LV +LPSYGRGRDAP GRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDKFHKNE NVTR
Sbjct: 131 LVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDKFHKNEFNVTR 190

Query: 184 PYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCT 243
           PY+IEIMYS+QIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC 
Sbjct: 191 PYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCI 250

Query: 244 NTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGG 303
           NTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGG
Sbjct: 251 NTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGG 310

Query: 304 IRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPE 363
           IR+VRIE+VTAINTQSAVRIKTA GRGG+VKDIFVR+MFLSTMKYVFWMTGDYKSHPD +
Sbjct: 311 IRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGDYKSHPDEK 370

Query: 364 FDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCT 423
           FDP A+PEIKNINYRDVVAENVTY+ARL+GISGDPFTDIC++N+ IGLT K KKLQWNCT
Sbjct: 371 FDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTAKQKKLQWNCT 430

Query: 424 NVEGFSSGVVPPPCAPLAKP-AEGGGCAFPTDRLPIEEFPLKTC 463
           NVEG+SS VVPPPCAPLAK      GC FP DRLPIE+  L TC
Sbjct: 431 NVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTC 474

[Close]

BLAST of MS017750 vs. ExPASy TrEMBL
Match: A0A0A0KNF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.3e-227
Identity = 380/460 (82.61%), Postives = 422/460 (91.74%), Query Frame = 0


Query: 7   AAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQA 66
           +AVILLAL+SLATV+G++ CP +N  + PAI+CRK++A++T+FG VGDGVTSNT AFR+A
Sbjct: 18  SAVILLALLSLATVEGKRSCP-TNYLQVPAISCRKHTALITDFGGVGDGVTSNTQAFRRA 77

Query: 67  IEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQ--DESEWPLVAIL 126
           IEHL  LAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA+IL SQ  +ESEWP VA+L
Sbjct: 78  IEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKILGSQVENESEWPQVALL 137

Query: 127 PSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIE 186
           PSYG GRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQG+YWWDKFHK ELN+TRPYLIE
Sbjct: 138 PSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWWDKFHKGELNLTRPYLIE 197

Query: 187 IMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIE 246
           IMYSDQIQIS+LTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPNTDGIDPDSC+NTRIE
Sbjct: 198 IMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPNTDGIDPDSCSNTRIE 257

Query: 247 DCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVR 306
           DCFIVSGDDCIAVKSGWDQYGI+FGMPTE L+I+RLTCISPDSAG+ALGSEMSGGIR+VR
Sbjct: 258 DCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGVALGSEMSGGIRNVR 317

Query: 307 IEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAA 366
           IE+ T INTQSAVRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA
Sbjct: 318 IENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAA 377

Query: 367 IPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGF 426
           +PEI NINYRDVVAENVTY+ARLEGISGDPFT+IC++NV IGLT  PKKLQWNCT+VEGF
Sbjct: 378 LPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTATPKKLQWNCTDVEGF 437

Query: 427 SSGVVPPPCAPLAKPAEGGGCAFPTDRLPIEEFPLKTCSI 465
           SS VVPPPCAPLAK  + GGC FP D+LPIE   LKTC +
Sbjct: 438 SSDVVPPPCAPLAKAGKSGGCDFPEDQLPIERVQLKTCFV 476

[Close]

BLAST of MS017750 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 306/468 (65.38%), Postives = 372/468 (79.49%), Query Frame = 0


Query: 5   VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 64
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 65  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 124
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 125 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 184
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 185 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRI 244
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+PDSCTNTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 245 EDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDV 304
           EDC+I+SGDDCIAVKSGWD+YGI FGMPT+HL+IRRLTCISP SA IALGSEMSGGI DV
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 305 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPA 364
           R ED+TA  T+S VRIKTA GRG FVK+I+V+ M L TMK+VFWMTG+YK+H D  +DP 
Sbjct: 305 RAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPH 364

Query: 365 AIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEG 424
           A+PEI  INYRD+VAENV+ A RLEGISGDPFT IC++N TI +  K KK  W C++VEG
Sbjct: 365 ALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEG 424

Query: 425 FSSGVVPPPCAPL--------AKPAEGGGCAFPTDRLPIEEFPLKTCS 464
            +SGV P PC  L         K    GGC FPTD L I+   LKTCS
Sbjct: 425 VTSGVDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCS 472

[Close]

BLAST of MS017750 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 306/468 (65.38%), Postives = 372/468 (79.49%), Query Frame = 0


Query: 5   VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 64
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 65  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 124
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 125 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 184
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 185 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRI 244
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+PDSCTNTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 245 EDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDV 304
           EDC+I+SGDDCIAVKSGWD+YGI FGMPT+HL+IRRLTCISP SA IALGSEMSGGI DV
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 305 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPA 364
           R ED+TA  T+S VRIKTA GRG FVK+I+V+ M L TMK+VFWMTG+YK+H D  +DP 
Sbjct: 305 RAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPH 364

Query: 365 AIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEG 424
           A+PEI  INYRD+VAENV+ A RLEGISGDPFT IC++N TI +  K KK  W C++VEG
Sbjct: 365 ALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEG 424

Query: 425 FSSGVVPPPCAPL--------AKPAEGGGCAFPTDRLPIEEFPLKTCS 464
            +SGV P PC  L         K    GGC FPTD L I+   LKTCS
Sbjct: 425 VTSGVDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCS 472

[Close]

BLAST of MS017750 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 306/468 (65.38%), Postives = 372/468 (79.49%), Query Frame = 0


Query: 5   VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 64
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 65  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 124
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 125 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 184
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 185 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPDSCTNTRI 244
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+PDSCTNTRI
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRI 244

Query: 245 EDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDV 304
           EDC+I+SGDDCIAVKSGWD+YGI FGMPT+HL+IRRLTCISP SA IALGSEMSGGI DV
Sbjct: 245 EDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDV 304

Query: 305 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPA 364
           R ED+TA  T+S VRIKTA GRG FVK+I+V+ M L TMK+VFWMTG+YK+H D  +DP 
Sbjct: 305 RAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPH 364

Query: 365 AIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEG 424
           A+PEI  INYRD+VAENV+ A RLEGISGDPFT IC++N TI +  K KK  W C++VEG
Sbjct: 365 ALPEITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEG 424

Query: 425 FSSGVVPPPCAPL--------AKPAEGGGCAFPTDRLPIEEFPLKTCS 464
            +SGV P PC  L         K    GGC FPTD L I+   LKTCS
Sbjct: 425 VTSGVDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCS 472

[Close]

BLAST of MS017750 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 644.4 bits (1661), Expect = 6.9e-185
Identity = 301/434 (69.35%), Postives = 357/434 (82.26%), Query Frame = 0


Query: 33  EFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS 92
           EF A+NCRK+SA+LT+FGAVGDG TSNT AFR AI  L  +A  GGAQL VP GKWLTGS
Sbjct: 34  EFSALNCRKHSAILTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGS 93

Query: 93  FNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPG-GRFSSLIYGTNLTDVV 152
           FNLTSHFTLF+ + A ILASQDESEWP++A LPSYG+GRD  G GRF+SLI GTNLTDVV
Sbjct: 94  FNLTSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVV 153

Query: 153 ITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYS 212
           ITGNNGTI+GQG YWWDKF K +  +TRPYLIEI++S  IQISN+TL++SPSW +HP+Y 
Sbjct: 154 ITGNNGTINGQGQYWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYC 213

Query: 213 SNVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMP 272
           ++VI++ +T+LAP+  PNTDGI+PDSCTNT IEDC+IVSGDDCIAVKSGWDQYGI+FGMP
Sbjct: 214 NSVIVKSVTVLAPVTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMP 273

Query: 273 TEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKD 332
           T+ L IRRLTCISP SAG+ALGSEMSGGI+DVRIEDVT  NT+SA+RIKTA GRG +VKD
Sbjct: 274 TQQLSIRRLTCISPKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKD 333

Query: 333 IFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGIS 392
           I+ RR+ + TMKYVFWM+G+Y SHPD  FDP A+PEI NINYRD+ AENVT +A L+GI 
Sbjct: 334 IYARRITMKTMKYVFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGID 393

Query: 393 GDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK---PAEGGGCAFP 452
            DPFT IC++NVTI L  K KK+QWNCT+V G +S V P PC+ L +    A+   CAFP
Sbjct: 394 KDPFTGICISNVTIALAAKAKKMQWNCTDVAGVTSRVTPEPCSLLPEKKAQAKNVDCAFP 453

Query: 453 TDRLPIEEFPLKTC 463
           +D +PIE   LK C
Sbjct: 454 SDLIPIESVVLKKC 467

[Close]

BLAST of MS017750 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 644.4 bits (1661), Expect = 6.9e-185
Identity = 303/432 (70.14%), Postives = 354/432 (81.94%), Query Frame = 0


Query: 33  EFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS 92
           E+ AI+CR YSA L EFGAVGDGVTSNT AFR A+  L   A  GG+ LFVPAG+WLTG+
Sbjct: 57  EYSAISCRAYSASLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRWLTGN 116

Query: 93  FNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVI 152
           FNLTSHFTLFLH+DA ILASQ+ES++ ++  LPSYGRGRD  GGRF SL++G+NLTDVVI
Sbjct: 117 FNLTSHFTLFLHRDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLTDVVI 176

Query: 153 TGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSS 212
           TG NGTIDGQG  WW KF + EL  TRPYLIEIM+SD IQISNLT +NSPSW +HP+YSS
Sbjct: 177 TGENGTIDGQGEPWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHPVYSS 236

Query: 213 NVIIQGLTILAPIDSPNTDGIDPDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPT 272
           N+ IQGLTILAP+  PNTDGI+PDSCTNTRIEDC+IVSGDDCIAVKSGWDQYGI +GMPT
Sbjct: 237 NIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKSGWDQYGINYGMPT 296

Query: 273 EHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 332
           + LLIRRLTCISPDSA IALGSEMSGGI DVR ED+ AIN++S +RIKTA GRGG+VKD+
Sbjct: 297 KQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRIKTAIGRGGYVKDV 356

Query: 333 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISG 392
           +VR M + TMKYVFWMTG Y SHPD  +DP A+P I+NINY+D+VAENVT  A+L GISG
Sbjct: 357 YVRGMTMMTMKYVFWMTGSYGSHPDDHYDPKALPVIQNINYQDMVAENVTMPAQLAGISG 416

Query: 393 DPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPC--APLAKPAEGGGCAFPTD 452
           D FT IC++NVTI L+KKPKK+ WNCT+V G++SGV P PC   P  +P     C FP D
Sbjct: 417 DQFTGICISNVTITLSKKPKKVLWNCTDVSGYTSGVTPQPCQLLPEKQPGTVVPCNFPED 476

Query: 453 RLPIEEFPLKTC 463
            +PI+E  L+ C
Sbjct: 477 PIPIDEVKLQRC 488

The following BLAST results are available for this feature:

BLAST of MS017750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906749.1	3.9e-238	86.24	probable polygalacturonase [Benincasa hispida]	[more]
XP_023532553.1	1.8e-235	86.21	probable polygalacturonase [Cucurbita pepo subsp. pepo]	[more]
XP_008437531.1	1.7e-233	85.37	PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative poly...	[more]
XP_022996001.1	3.7e-233	85.78	probable polygalacturonase [Cucurbita maxima]	[more]
XP_022957618.1	1.1e-232	84.48	probable polygalacturonase [Cucurbita moschata]	[more]

BLAST of MS017750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	7.2e-179	64.44	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q9LW07	4.3e-30	28.78	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q7M1E7	1.1e-28	29.66	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9FY19	7.5e-27	28.75	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]
Q8RY29	2.9e-26	26.57	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]

BLAST of MS017750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C461	8.1e-234	85.37	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3AUV5	8.1e-234	85.37	probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1	[more]
A0A6J1K3G8	1.8e-233	85.78	probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1	[more]
A0A6J1H0R5	5.3e-233	84.48	probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV...	[more]
A0A0A0KNF9	3.3e-227	82.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1	[more]

BLAST of MS017750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61490.1	1.1e-185	65.38	Pectin lyase-like superfamily protein	[more]
AT3G61490.2	1.1e-185	65.38	Pectin lyase-like superfamily protein	[more]
AT3G61490.3	1.1e-185	65.38	Pectin lyase-like superfamily protein	[more]
AT3G48950.1	6.9e-185	69.35	Pectin lyase-like superfamily protein	[more]
AT4G23500.1	6.9e-185	70.14	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 238..259 e-value: 430.0 score: 9.7 coord: 300..321 e-value: 560.0 score: 9.0 coord: 272..294 e-value: 2800.0 score: 4.0 coord: 211..237 e-value: 1000.0 score: 7.2
IPR024535	Pectate lyase superfamily protein	PFAM	PF12708	Pectate_lyase_3	coord: 47..101 e-value: 1.9E-6 score: 27.9
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 140..422 e-value: 2.7E-42 score: 145.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 28..426 e-value: 5.1E-117 score: 393.3
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 8..463
None	No IPR available	PANTHER	PTHR31339:SF12	ENDO-POLYGALACTURONASE-LIKE PROTEIN	coord: 8..463
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 42..454

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017750.1	MS017750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity