MS017739 (gene) Bitter gourd (TR) v1

Overview


Name	MS017739
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Beta-carotene hydroxylase
Location	scaffold373: 2034482 .. 2036464 (-)
RNA-Seq Expression	MS017739
Synteny	MS017739

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACGGGGATCTCGGGCGTGGCAACCGCCGCAGCGTACCGGTTCAACCCGGGGCTGTTTCCGGATGCTCCGAAAATCGTAGCGAAGAGGACCCCACGTTTCCCGCGGCGGTGCTCGAGGCAGAGGTGGAAGAGATTGTCGGCCGGCGGCGGAGGAGATGACGTTAATGAATTGGAAGTGGTGAATAAGGTTTCGAATGCGACTGTGGAGGAGAAATTGGCCAGGAAGAAATTGGAAAGAGAAACTTATCTTGTCGCTGCAATTGTATCGAGCTTCGGGATTACTTCAATGGCCGCCGTCGCCGTTTGTTACAGATTCTCATGTCAAATGGGGGTATTTTTTCCTTTATTTTTTTTATTTTTTTTAATTTTTCCTCCTTCCAATATTCTCGGAGTTCTAAATGGTCTAATCTATTAATCTTTTAAAAGTATCCATTTGAATATTTAATTAAATTCTTTTATAATATAAAAAATATTTCATTGGTCATTATTTTCTAAAAAAGATGTACTAAATAGGCTCATATTCGGATGTATTAATAAAATAATAAATAATCCATTTACAGAACGGAGAGTTTCCAGTAGCTGAAATGTTGGGTACTTTCGCTCTCTCTATTGGCGCTGCGGTGAGTGTGTGAGTGGAGTTAAATTATCGAATATTAGATGAAATCCCCTTTTAAGGACCTTTTTGGTTTTTTAATTTTGATTTTTGATTTTAGGTGGGAATGGAATTTTGGGCAAGATGGGCTCATAGAGCGCTGTGGCATGCTTCATTATGGAATATGCACCAGGTGGCGTTACATTAAATGCGTGGATTATGATAATTTAAAGCTAAGTTAAATAATGTCAAAAAGCATATAGTATAAAGTTAAAAGTAAGCAAGTTATATATATGTCTGTGGCTCTTCTTTTGGTGTGTAGTCTCATCACAGGCCTAGAAATGGAGCATTTGAACTAAACGACGTGTTTGTGATTATCAACGCTGTGCCTGCCATTGCTCTTATTTCCTTCGGCCTCTACAATAAAGGCTTCATTCCAGGCCTCTGCTTTGGTGCCGTATGTTCATTTTAACACTTTTTTTCTCTTTTATCCCAAACTTTCAAAATTATAAAACTTCAAATGTTATTCAACTTTAAAAATGTGTAAGGTATCTTTTAACTTTAAATTCTTAGAGGCTTTCTTTTACTTATTAGACACAAATTTGACCTTAATCTTCTAAATTTCTAATTTATTAAATACAAAATTAAAAATTTTGAATTCTAATAACTTGTTAGAACACAAAATTGTCATTTTAGGAACTAAAGTTAAAATTTAACTTCGGGCTAATATTAAAATAGTTTAAATACTATTTTAGTCTTACTTTTTAGAGTTTGATTCATTTTGATTATTGTATTATTCTTTACTTTTAATTTTTATTTATTTTAATCTCTATGCTGTGAGATCGTGGATTTTGGTCTTTGTGCTCCCTTAATTTTAATTTTTATTTCAATCAAAAACATTTAAATCTATTAAAAAATTCGATATTTTTAATGTTTTCATCCTAATTGGACTAAAACTGATAGAAGTTCCCACTTTCCGACCTAATCTAGTAAAAGTGAAGGCTATTGAAATCCAAGTCAGCTATGTATGACGTGTCAAATTATGATTGGCTATTGACTATTTAATAATATTCGGCAGGGTTTGGGCATTACGGTCTTCGGTATGGCTTATATGTTCGTACACGACGGCCTCGTGCACCGTCGTTTCCCGGTGGGCCCCATAGCAGCTGTCCCCTATTTACGACGTGTCGCGGCTGCCCATCATGTACTTCTTTCATCACTCGTAGCTTTCTACGACATGTCGCCTGCTTCAGTACAACTTTTATTATTATTATTATTATTATTAATATAATATGATATTTGTTTGGGGTAATTTTAAATTTTGGGCAGATTCATCACACGGACAAATTCAACGGAGTCCCATACGGATTGTTTTTAGGACCCAAG

mRNA sequence

ATGGCGACGGGGATCTCGGGCGTGGCAACCGCCGCAGCGTACCGGTTCAACCCGGGGCTGTTTCCGGATGCTCCGAAAATCGTAGCGAAGAGGACCCCACGTTTCCCGCGGCGGTGCTCGAGGCAGAGGTGGAAGAGATTGTCGGCCGGCGGCGGAGGAGATGACGTTAATGAATTGGAAGTGGTGAATAAGGTTTCGAATGCGACTGTGGAGGAGAAATTGGCCAGGAAGAAATTGGAAAGAGAAACTTATCTTGTCGCTGCAATTGTATCGAGCTTCGGGATTACTTCAATGGCCGCCGTCGCCGTTTGTTACAGATTCTCATGTCAAATGGGGAACGGAGAGTTTCCAGTAGCTGAAATGTTGGGTACTTTCGCTCTCTCTATTGGCGCTGCGGTGGGAATGGAATTTTGGGCAAGATGGGCTCATAGAGCGCTGTGGCATGCTTCATTATGGAATATGCACCAGTCTCATCACAGGCCTAGAAATGGAGCATTTGAACTAAACGACGTGTTTGTGATTATCAACGCTGTGCCTGCCATTGCTCTTATTTCCTTCGGCCTCTACAATAAAGGCTTCATTCCAGGCCTCTGCTTTGGTGCCGGTTTGGGCATTACGGTCTTCGGTATGGCTTATATGTTCGTACACGACGGCCTCGTGCACCGTCGTTTCCCGGTGGGCCCCATAGCAGCTGTCCCCTATTTACGACGTGTCGCGGCTGCCCATCATATTCATCACACGGACAAATTCAACGGAGTCCCATACGGATTGTTTTTAGGACCCAAG

Coding sequence (CDS)

ATGGCGACGGGGATCTCGGGCGTGGCAACCGCCGCAGCGTACCGGTTCAACCCGGGGCTGTTTCCGGATGCTCCGAAAATCGTAGCGAAGAGGACCCCACGTTTCCCGCGGCGGTGCTCGAGGCAGAGGTGGAAGAGATTGTCGGCCGGCGGCGGAGGAGATGACGTTAATGAATTGGAAGTGGTGAATAAGGTTTCGAATGCGACTGTGGAGGAGAAATTGGCCAGGAAGAAATTGGAAAGAGAAACTTATCTTGTCGCTGCAATTGTATCGAGCTTCGGGATTACTTCAATGGCCGCCGTCGCCGTTTGTTACAGATTCTCATGTCAAATGGGGAACGGAGAGTTTCCAGTAGCTGAAATGTTGGGTACTTTCGCTCTCTCTATTGGCGCTGCGGTGGGAATGGAATTTTGGGCAAGATGGGCTCATAGAGCGCTGTGGCATGCTTCATTATGGAATATGCACCAGTCTCATCACAGGCCTAGAAATGGAGCATTTGAACTAAACGACGTGTTTGTGATTATCAACGCTGTGCCTGCCATTGCTCTTATTTCCTTCGGCCTCTACAATAAAGGCTTCATTCCAGGCCTCTGCTTTGGTGCCGGTTTGGGCATTACGGTCTTCGGTATGGCTTATATGTTCGTACACGACGGCCTCGTGCACCGTCGTTTCCCGGTGGGCCCCATAGCAGCTGTCCCCTATTTACGACGTGTCGCGGCTGCCCATCATATTCATCACACGGACAAATTCAACGGAGTCCCATACGGATTGTTTTTAGGACCCAAG

Protein sequence

MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELEVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAEMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPAIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFNGVPYGLFLGPK

Homology

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BLAST of MS017739 vs. NCBI nr
Match: XP_022159511.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 527.3 bits (1357), Expect = 7.4e-146
Identity = 259/262 (98.85%), Postives = 259/262 (98.85%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELE 60
           MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPR PRRCSRQRWKRLSAGGGGDDVNELE
Sbjct: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRVPRRCSRQRWKRLSAGGGGDDVNELE 60

Query: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAE 120
           VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPV E
Sbjct: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVVE 120

Query: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180
           MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA
Sbjct: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180

Query: 181 IALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALISFGL NKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALISFGLCNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHHIHHTDKFNGVPYGLFLGPK 263
           AHHIHHTDKFNGVPYGLFLGPK
Sbjct: 241 AHHIHHTDKFNGVPYGLFLGPK 262

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BLAST of MS017739 vs. NCBI nr
Match: XP_022159510.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 516.9 bits (1330), Expect = 1.0e-142
Identity = 259/278 (93.17%), Postives = 259/278 (93.17%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELE 60
           MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPR PRRCSRQRWKRLSAGGGGDDVNELE
Sbjct: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRVPRRCSRQRWKRLSAGGGGDDVNELE 60

Query: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAE 120
           VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPV E
Sbjct: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVVE 120

Query: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180
           MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA
Sbjct: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180

Query: 181 IALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALISFGL NKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALISFGLCNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHH----------------IHHTDKFNGVPYGLFLGPK 263
           AHH                IHHTDKFNGVPYGLFLGPK
Sbjct: 241 AHHVLLSSLVAFYDMSPASIHHTDKFNGVPYGLFLGPK 278

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BLAST of MS017739 vs. NCBI nr
Match: XP_022159512.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X3 [Momordica charantia])

HSP 1 Score: 468.4 bits (1204), Expect = 4.1e-128
Identity = 240/278 (86.33%), Postives = 240/278 (86.33%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELE 60
           MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPR PRRCSRQRWKRLSAGGGGDDVNELE
Sbjct: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRVPRRCSRQRWKRLSAGGGGDDVNELE 60

Query: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAE 120
           VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMG        
Sbjct: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMG-------- 120

Query: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180
                       VGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA
Sbjct: 121 ------------VGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180

Query: 181 IALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALISFGL NKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALISFGLCNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHH----------------IHHTDKFNGVPYGLFLGPK 263
           AHH                IHHTDKFNGVPYGLFLGPK
Sbjct: 241 AHHVLLSSLVAFYDMSPASIHHTDKFNGVPYGLFLGPK 258

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BLAST of MS017739 vs. NCBI nr
Match: XP_022929023.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG7016987.1 Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 371.3 bits (952), Expect = 6.8e-99
Identity = 191/263 (72.62%), Postives = 212/263 (80.61%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRC-SRQRWKRLSAGGGGDDVNEL 60
           MATGIS VATAA YR         P        R P RC ++Q  +RLS       V+E+
Sbjct: 1   MATGISAVATAAPYR----RLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEV 60

Query: 61  EVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVA 120
           E+V K     VEEK+ RKK ER+ YLVAAIVSSFGITSMAA+AV YRFS Q+  G+FPV 
Sbjct: 61  EIV-KDLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVL 120

Query: 121 EMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVP 180
           EM+GTFALS+GAAVGMEFWARWAH+ LWH SLWNMH+SHHR RNG FELNDVF IINAVP
Sbjct: 121 EMVGTFALSVGAAVGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVP 180

Query: 181 AIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240
           AIAL+SFG +++GF+PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA
Sbjct: 181 AIALLSFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240

Query: 241 AAHHIHHTDKFNGVPYGLFLGPK 263
           AAHHIHHTDKF+GVPYGLFLGPK
Sbjct: 241 AAHHIHHTDKFDGVPYGLFLGPK 258

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BLAST of MS017739 vs. NCBI nr
Match: KAG6579527.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.8 bits (948), Expect = 2.0e-98
Identity = 190/263 (72.24%), Postives = 211/263 (80.23%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRC-SRQRWKRLSAGGGGDDVNEL 60
           MATGIS VATAA YR         P        R P RC ++Q  +RLS       V+E+
Sbjct: 1   MATGISAVATAAPYR----RLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEV 60

Query: 61  EVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVA 120
           E+V K     VEEK+ RKK ER+ YLVAAIVSSFGITSMAA+AV YRFS Q+  G+FPV 
Sbjct: 61  EIV-KDLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVL 120

Query: 121 EMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVP 180
           EM+GTF LS+GAAVGMEFWARWAH+ LWH SLWNMH+SHHR RNG FELNDVF IINAVP
Sbjct: 121 EMVGTFTLSVGAAVGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVP 180

Query: 181 AIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240
           AIAL+SFG +++GF+PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA
Sbjct: 181 AIALLSFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240

Query: 241 AAHHIHHTDKFNGVPYGLFLGPK 263
           AAHHIHHTDKF+GVPYGLFLGPK
Sbjct: 241 AAHHIHHTDKFDGVPYGLFLGPK 258

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BLAST of MS017739 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 333.6 bits (854), Expect = 2.1e-90
Identity = 156/210 (74.29%), Postives = 183/210 (87.14%), Query Frame = 0


Query: 53  GDDVNELEVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMG 112
           G++  E+++  ++S   + EKLARKK ER TYLVAA++SSFGITSMA +AV YRF  QM 
Sbjct: 74  GEEDIEMKIEEQISATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQME 133

Query: 113 NGEFPVAEMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVF 172
            GE P +EM GTFALS+GAAVGMEFWARWAH+ALWHASLW+MH+SHH+PR G FELNDVF
Sbjct: 134 GGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVF 193

Query: 173 VIINAVPAIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAV 232
            IINAVPAIAL+ +G ++KG IPGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGP+A V
Sbjct: 194 AIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANV 253

Query: 233 PYLRRVAAAHHIHHTDKFNGVPYGLFLGPK 263
           PYLR+VAAAH +HH++KFNGVPYGLFLGPK
Sbjct: 254 PYLRKVAAAHSLHHSEKFNGVPYGLFLGPK 283

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BLAST of MS017739 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 7.8e-90
Identity = 158/212 (74.53%), Postives = 180/212 (84.91%), Query Frame = 0


Query: 51  GGGDDVNELEVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQ 110
           G G+   + E     S   + +KLARKK ER TYLVAA++SSFGITSMA ++V YRFS Q
Sbjct: 85  GAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQ 144

Query: 111 MGNGEFPVAEMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELND 170
           M  GE P++EM GTFALS+GAAVGMEFWARWAH ALWHASLW+MH+SHH+PR G FELND
Sbjct: 145 MEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPFELND 204

Query: 171 VFVIINAVPAIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIA 230
           +F IINAVPAIAL+S+G ++KG IPGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA
Sbjct: 205 IFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIA 264

Query: 231 AVPYLRRVAAAHHIHHTDKFNGVPYGLFLGPK 263
            VPY RRVAAAH +HH+DKFNGVPYGLFLGPK
Sbjct: 265 DVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPK 296

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BLAST of MS017739 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 6.6e-89
Identity = 153/191 (80.10%), Postives = 170/191 (89.01%), Query Frame = 0


Query: 72  EKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAEMLGTFALSIGA 131
           EKL RKK ER TYL+AA++SSFGITSMA +AV YRFS QM  GE  + EM GTFALS+GA
Sbjct: 86  EKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGA 145

Query: 132 AVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPAIALISFGLYNK 191
           AVGMEFWARWAHRALWHASLWNMH+SHH+PR G FELNDVF I+NA PAI L+S+G +NK
Sbjct: 146 AVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNK 205

Query: 192 GFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFN 251
           G +PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKFN
Sbjct: 206 GLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFN 265

Query: 252 GVPYGLFLGPK 263
           GVPYGLFLGPK
Sbjct: 266 GVPYGLFLGPK 276

[Close]

BLAST of MS017739 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-86
Identity = 160/239 (66.95%), Postives = 189/239 (79.08%), Query Frame = 0


Query: 25  PKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELEVVNKVSNAT-VEEKLARKKLERET 84
           P++ A    +  +  + Q  K+      GD++ E+E   + S A  + EK ARKK ER T
Sbjct: 53  PRLAACFVLKDDKLYTAQSGKQSDTEAIGDEI-EVETNEEKSLAVRLAEKFARKKSERFT 112

Query: 85  YLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAEMLGTFALSIGAAVGMEFWARWAH 144
           YLVAA++SS GITSMA ++V YRFS QM  GE P +EM  TFAL+ GAA+GME+WARWAH
Sbjct: 113 YLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAAIGMEYWARWAH 172

Query: 145 RALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPAIALISFGLYNKGFIPGLCFGAGL 204
           RALWHASLW+MH+SHHRPR G FELND+F IINAVPAIAL+SFG  +KG IPGLCFGAGL
Sbjct: 173 RALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIALLSFGFNHKGLIPGLCFGAGL 232

Query: 205 GITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFNGVPYGLFLGPK 263
           GITVFGMAYMFVHDGLVH+RFPVGPIA VPY +RVAAAH +HH+DKF+GVPYGLFLGPK
Sbjct: 233 GITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQLHHSDKFDGVPYGLFLGPK 290

[Close]

BLAST of MS017739 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.0e-86
Identity = 155/213 (72.77%), Postives = 173/213 (81.22%), Query Frame = 0


Query: 54  DDVNELEVVNKVSNATVEEKL----ARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSC 113
           DD N+ E     S   +  +L     +KK ER TYL+AA++SSFGITSMA +AV YRFS 
Sbjct: 62  DDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAIMAVYYRFSW 121

Query: 114 QMGNGEFPVAEMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELN 173
           QM  GE  V EM GTFALS+GAAVGMEFWARWAHRALWH SLWNMH+SHH+PR GAFELN
Sbjct: 122 QMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELN 181

Query: 174 DVFVIINAVPAIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPI 233
           DVF I NAVPAI L+ +G  NKG +PGLCFGAGLGIT+FGMAYMFVHDGLVH+RFPVGPI
Sbjct: 182 DVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPI 241

Query: 234 AAVPYLRRVAAAHHIHHTDKFNGVPYGLFLGPK 263
           A VPYLR+VAAAH +HHTDKF GVPYGLFLGPK
Sbjct: 242 ANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPK 274

[Close]

BLAST of MS017739 vs. ExPASy TrEMBL
Match: A0A6J1E454 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025906 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.6e-146
Identity = 259/262 (98.85%), Postives = 259/262 (98.85%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELE 60
           MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPR PRRCSRQRWKRLSAGGGGDDVNELE
Sbjct: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRVPRRCSRQRWKRLSAGGGGDDVNELE 60

Query: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAE 120
           VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPV E
Sbjct: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVVE 120

Query: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180
           MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA
Sbjct: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180

Query: 181 IALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALISFGL NKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALISFGLCNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHHIHHTDKFNGVPYGLFLGPK 263
           AHHIHHTDKFNGVPYGLFLGPK
Sbjct: 241 AHHIHHTDKFNGVPYGLFLGPK 262

[Close]

BLAST of MS017739 vs. ExPASy TrEMBL
Match: A0A6J1DYY1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025906 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 4.8e-143
Identity = 259/278 (93.17%), Postives = 259/278 (93.17%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELE 60
           MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPR PRRCSRQRWKRLSAGGGGDDVNELE
Sbjct: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRVPRRCSRQRWKRLSAGGGGDDVNELE 60

Query: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAE 120
           VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPV E
Sbjct: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVVE 120

Query: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180
           MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA
Sbjct: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180

Query: 181 IALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALISFGL NKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALISFGLCNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHH----------------IHHTDKFNGVPYGLFLGPK 263
           AHH                IHHTDKFNGVPYGLFLGPK
Sbjct: 241 AHHVLLSSLVAFYDMSPASIHHTDKFNGVPYGLFLGPK 278

[Close]

BLAST of MS017739 vs. ExPASy TrEMBL
Match: A0A6J1E019 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025906 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.0e-128
Identity = 240/278 (86.33%), Postives = 240/278 (86.33%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRCSRQRWKRLSAGGGGDDVNELE 60
           MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPR PRRCSRQRWKRLSAGGGGDDVNELE
Sbjct: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRVPRRCSRQRWKRLSAGGGGDDVNELE 60

Query: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAE 120
           VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMG        
Sbjct: 61  VVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMG-------- 120

Query: 121 MLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180
                       VGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA
Sbjct: 121 ------------VGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPA 180

Query: 181 IALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240
           IALISFGL NKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA
Sbjct: 181 IALISFGLCNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAA 240

Query: 241 AHH----------------IHHTDKFNGVPYGLFLGPK 263
           AHH                IHHTDKFNGVPYGLFLGPK
Sbjct: 241 AHHVLLSSLVAFYDMSPASIHHTDKFNGVPYGLFLGPK 258

[Close]

BLAST of MS017739 vs. ExPASy TrEMBL
Match: A0A6J1EQT9 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435746 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.3e-99
Identity = 191/263 (72.62%), Postives = 212/263 (80.61%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRC-SRQRWKRLSAGGGGDDVNEL 60
           MATGIS VATAA YR         P        R P RC ++Q  +RLS       V+E+
Sbjct: 1   MATGISAVATAAPYR----RLIQRPAKAPPPANRVPWRCFAKQSTRRLSTVKKKSGVSEV 60

Query: 61  EVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVA 120
           E+V K     VEEK+ RKK ER+ YLVAAIVSSFGITSMAA+AV YRFS Q+  G+FPV 
Sbjct: 61  EIV-KDLKVIVEEKMVRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLKGGDFPVL 120

Query: 121 EMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVP 180
           EM+GTFALS+GAAVGMEFWARWAH+ LWH SLWNMH+SHHR RNG FELNDVF IINAVP
Sbjct: 121 EMVGTFALSVGAAVGMEFWARWAHKQLWHDSLWNMHKSHHRARNGPFELNDVFAIINAVP 180

Query: 181 AIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240
           AIAL+SFG +++GF+PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA
Sbjct: 181 AIALLSFGFFHQGFVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240

Query: 241 AAHHIHHTDKFNGVPYGLFLGPK 263
           AAHHIHHTDKF+GVPYGLFLGPK
Sbjct: 241 AAHHIHHTDKFDGVPYGLFLGPK 258

[Close]

BLAST of MS017739 vs. ExPASy TrEMBL
Match: A0A6J1I1U3 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.1e-98
Identity = 190/263 (72.24%), Postives = 211/263 (80.23%), Query Frame = 0


Query: 1   MATGISGVATAAAYRFNPGLFPDAPKIVAKRTPRFPRRC-SRQRWKRLSAGGGGDDVNEL 60
           MATGIS VATAA YR         P        R P RC ++Q  +RLS       V+E+
Sbjct: 1   MATGISAVATAAPYR----RIIQRPAKAPAPANRVPWRCFAKQSTRRLSTVKKKCGVSEV 60

Query: 61  EVVNKVSNATVEEKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVA 120
           E+V  +    VEEK+ RKK ER+ YLVAAIVSSFGITSMAA+AV YRFS Q+  G+FPV 
Sbjct: 61  EMVRDL-KVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEGGDFPVL 120

Query: 121 EMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVP 180
           EM GTFALS+GAAVGMEFWARWAH+ LWH +LWNMH+SHHR R+GAFELNDVF IINAVP
Sbjct: 121 EMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVP 180

Query: 181 AIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240
           AIAL+SFG +N+GF PGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA
Sbjct: 181 AIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 240

Query: 241 AAHHIHHTDKFNGVPYGLFLGPK 263
           AAHHIHHTDKF+GVPYGLFLGPK
Sbjct: 241 AAHHIHHTDKFDGVPYGLFLGPK 258

[Close]

BLAST of MS017739 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 328.6 bits (841), Expect = 4.7e-90
Identity = 153/191 (80.10%), Postives = 170/191 (89.01%), Query Frame = 0


Query: 72  EKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAEMLGTFALSIGA 131
           EKL RKK ER TYL+AA++SSFGITSMA +AV YRFS QM  GE  + EM GTFALS+GA
Sbjct: 86  EKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGA 145

Query: 132 AVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPAIALISFGLYNK 191
           AVGMEFWARWAHRALWHASLWNMH+SHH+PR G FELNDVF I+NA PAI L+S+G +NK
Sbjct: 146 AVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNK 205

Query: 192 GFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFN 251
           G +PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKFN
Sbjct: 206 GLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFN 265

Query: 252 GVPYGLFLGPK 263
           GVPYGLFLGPK
Sbjct: 266 GVPYGLFLGPK 276

[Close]

BLAST of MS017739 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 319.3 bits (817), Expect = 2.9e-87
Identity = 155/213 (72.77%), Postives = 173/213 (81.22%), Query Frame = 0


Query: 54  DDVNELEVVNKVSNATVEEKL----ARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSC 113
           DD N+ E     S   +  +L     +KK ER TYL+AA++SSFGITSMA +AV YRFS 
Sbjct: 62  DDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAIMAVYYRFSW 121

Query: 114 QMGNGEFPVAEMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELN 173
           QM  GE  V EM GTFALS+GAAVGMEFWARWAHRALWH SLWNMH+SHH+PR GAFELN
Sbjct: 122 QMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELN 181

Query: 174 DVFVIINAVPAIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPI 233
           DVF I NAVPAI L+ +G  NKG +PGLCFGAGLGIT+FGMAYMFVHDGLVH+RFPVGPI
Sbjct: 182 DVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPI 241

Query: 234 AAVPYLRRVAAAHHIHHTDKFNGVPYGLFLGPK 263
           A VPYLR+VAAAH +HHTDKF GVPYGLFLGPK
Sbjct: 242 ANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPK 274

[Close]

BLAST of MS017739 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 208.4 bits (529), Expect = 7.1e-54
Identity = 98/130 (75.38%), Postives = 111/130 (85.38%), Query Frame = 0


Query: 72  EKLARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSCQMGNGEFPVAEMLGTFALSIGA 131
           EKL RKK ER TYL+AA++SSFGITSMA +AV YRFS QM  GE  + EM GTFALS+GA
Sbjct: 86  EKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGA 145

Query: 132 AVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELNDVFVIINAVPAIALISFGLYNK 191
           AVGMEFWARWAHRALWHASLWNMH+SHH+PR G FELNDVF I+NA PAI L+S+G +NK
Sbjct: 146 AVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNK 205

Query: 192 GFIPGLCFGA 202
           G +PGLCFGA
Sbjct: 206 GLVPGLCFGA 215

[Close]

BLAST of MS017739 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 165.6 bits (418), Expect = 5.3e-41
Identity = 100/213 (46.95%), Postives = 113/213 (53.05%), Query Frame = 0


Query: 54  DDVNELEVVNKVSNATVEEKL----ARKKLERETYLVAAIVSSFGITSMAAVAVCYRFSC 113
           DD N+ E     S   +  +L     +KK ER TYL+AA++SSFGITSMA +AV YRFS 
Sbjct: 62  DDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAIMAVYYRFSW 121

Query: 114 QMGNGEFPVAEMLGTFALSIGAAVGMEFWARWAHRALWHASLWNMHQSHHRPRNGAFELN 173
           QM  GE  V EM GTFALS+GA                                      
Sbjct: 122 QMKGGEVSVLEMFGTFALSVGA-------------------------------------- 181

Query: 174 DVFVIINAVPAIALISFGLYNKGFIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPI 233
                                          AGLGIT+FGMAYMFVHDGLVH+RFPVGPI
Sbjct: 182 -------------------------------AGLGITMFGMAYMFVHDGLVHKRFPVGPI 205

Query: 234 AAVPYLRRVAAAHHIHHTDKFNGVPYGLFLGPK 263
           A VPYLR+VAAAH +HHTDKF GVPYGLFLGPK
Sbjct: 242 ANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPK 205

The following BLAST results are available for this feature:

BLAST of MS017739 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159511.1	7.4e-146	98.85	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 [Momordica charanti...	[more]
XP_022159510.1	1.0e-142	93.17	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Momordica charanti...	[more]
XP_022159512.1	4.1e-128	86.33	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X3 [Momordica charanti...	[more]
XP_022929023.1	6.8e-99	72.62	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Cucurbita moschata...	[more]
KAG6579527.1	2.0e-98	72.24	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]

BLAST of MS017739 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	2.1e-90	74.29	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	7.8e-90	74.53	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	6.6e-89	80.10	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	1.4e-86	66.95	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	4.0e-86	72.77	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of MS017739 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E454	3.6e-146	98.85	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Momordica charan...	[more]
A0A6J1DYY1	4.8e-143	93.17	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Momordica charan...	[more]
A0A6J1E019	2.0e-128	86.33	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X3 OS=Momordica charan...	[more]
A0A6J1EQT9	3.3e-99	72.62	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita moscha...	[more]
A0A6J1I1U3	2.1e-98	72.24	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima...	[more]

BLAST of MS017739 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	4.7e-90	80.10	beta-hydroxylase 1	[more]
AT5G52570.1	2.9e-87	72.77	beta-carotene hydroxylase 2	[more]
AT4G25700.2	7.1e-54	75.38	beta-hydroxylase 1	[more]
AT5G52570.2	5.3e-41	46.95	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 131..258 e-value: 2.2E-13 score: 50.7
None	No IPR available	PANTHER	PTHR31899:SF14	HYDROXYLASE, PUTATIVE, EXPRESSED-RELATED	coord: 35..262
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 35..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017739.1	MS017739.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity