MS017712 (gene) Bitter gourd (TR) v1

Overview


Name	MS017712
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Tyrosine--tRNA ligase
Location	scaffold373: 1810985 .. 1813516 (+)
RNA-Seq Expression	MS017712
Synteny	MS017712

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACGGAAGCGCCTTCACAGGCCCCATCCGAGGATATACAGTCCCTCTCAATCTCCGACGCTTCAGTGCAAGAGCCCGGTTGCTCATCGAACTCTTCATCAAACCAGTAAGAAATTTTTGATTCGAGATACTTGTTTTCGCATCTATATGGCCGTTGATTTGTGTTCTGAATCGGATATTACTTGCTCTGTTACGTATTTTTTCTTCAGATTGAGCTTGGACGAGAAGTTTCGAATTGTTAGAAGTGTTGGGGAAGAGTGTATCCAAGAAGATGAGCTGCGGAACCTGCTGGAGAAGAAGCCTGAACCACTGTGCTATGACGGCTTCGAACCCTCTGGGAGAATGCATATTGCTCAGGTATTGTTTTGAGGAATCTCTGCGCCAGGCCTTCAAATTACAATTTACTTTCTTCAATGAGTCGCGTTGCATTTGGATGCATAGTGGAAACTCTAGGTGTAGTTTCTACACTCAGTGAATTGGGATTTAGTTTTCGGTGCCAATGGTATCCTGTTGACAGTTTAAATTTCAGCAGGCAAAATGGCAAACTAAGTAAGTAATACATTAATCTAGAATGCAACGAAAATTAAACAATTCCCAAGTAAGTTTATTCCACTCAAGGGTCCTTTGAATACGAGAAGCTTATTTTTGCCCTCGTTCGCTTGCACAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGGTGCAGAGTGAAAATATGGATTGCAGATTGGTTTGCTCAGCTAAACAATAAAATGGGGGGCGATCTGAAGAAAATTGAGACTGTTGGCCAGTACCTGATCGAGATATGGAAAGCAGTGGGGATGGATCTGGAAGGAGGCAAGGTCGAATTTTTGTGGTCGTCCAAGGAGATTAACTCAAGGGCATCAGAATACTGGCCCCTTGTAATGGATATTGCTCGGAGGAACAAGCTTCCAAGGATAATTAGGTATAAATATAATATTAGAGTCTGGTGCATTTTGTGTTCAATCTGCAACAGTTTGACTCATCGTTTGCTTCTTGGCTTCCCTCCCTCTCCTGCAGGTGTAGCCAAATTATGGGAAGAAATGAGCAGGATGAGTTGAGCGCCGCCCAAATTTTCTATCCATGCATGCAATGTGCCGACATTTTTTTCCTGAAGGTTTTTCTCCTTGTGATTTATCTTTCCAGTATTTTGTTGGTGTGCATTTTTCTTCTCATATTATAAGGAGGCAAAGATCTAATTTGGTATGTATGCTGCAAAATATATTTGTCTGGCTATGATATTTTTATGGTGATATAGGCTGTCCATTTCTTTTACTCTCCTCTCCTATACTGGGATGTACGGAGAGATTACCATATTTTTTTGTCAGCTCGTTGTTTAATTCCATTGCAACAGGCTGATATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTACTTGCAAGAGAGTATTGTGATGATATCAAGAGGAAGAACAAGCCTATCATCCTATCACACCGTATGCTCTTTGCACTGACATTACTTCTTACTGATGCTCATGATATAAGCAAAGTAATATGATGCCAATGCTGGTTGCAGACATGTTACCGGGCTTACAGCAAGGGCAGGAGAAGATGTCAAAAAGTGATCCGTCATCCTCTATATTTATGGAAGATGATGAGGTAAGGCATCATATAGTGGAAATTAAGAATCCCATGTGAGGAGGGGAACTGAAATGCATCTGTCATTTTTACGTTGAGTTCAGTTTTAGGTTGCCTGAAGTGTCTGGCTCTAACATTGAGGCTAACATATGATATTCTGGATTAATGTCTATCACGTTCATCTGGTTATCTCAGGCGGAAGTGAATGTGAAGATAAAGAAAGCATACTGCCCTCCATTGATCGTGGAAGGGAATCCATGCCTGGAGTACATTAAGTATCTTATTTTCCCTTGGTTCAAGGAGTTCACGGTGGAACGAAGTGCTGATCATGGTGGAAATAAGTAAGCTTTTCTGTTCATTCAATTTCTCACTTTAGAAGTTTCAAATTAATTCTTGTTTCTTTCACCTATGTCATGATCCCAGATGTTGGTTATAATCATTTATCTTGGCCCCCTCTTTGTTTGTCGTCTCACATTACAGGACCTTCACAAGCTTCGAAGAACTGGTTGCTGACTACGAAACAGGAGTACTGCATCCAGGTGATCTCAAACCAGCATTGTCTAAGGCTTTGAATAAGATACTGGAGGTATGTTTTCATTTCTACTCAATATCTTCAAATAAAATACAGTTTCTTTACTATTCATTCATGCTCTTGCAATTTCATCTGATAAAGAAGCACCTAGCATAACTTAAGAAGCTTAATTCCTGTTATGAAAATTCAAGCAAAATTTTCAGCGATAGGGTAGTGATGCAGCTAGAATTTTTTCTAGATTATACTTGCATTATAAAGCTAAGATCTGTATCATCCATCTGCAGCCTGTACGAGAGCACTTCAAAAATGATAGCAGAGCCAAAGATTTATTAAAGAGGGTCAAGGTATGTGTTTGGCTTAAT

mRNA sequence

ATGGCGACGGAAGCGCCTTCACAGGCCCCATCCGAGGATATACAGTCCCTCTCAATCTCCGACGCTTCAGTGCAAGAGCCCGGTTGCTCATCGAACTCTTCATCAAACCAATTGAGCTTGGACGAGAAGTTTCGAATTGTTAGAAGTGTTGGGGAAGAGTGTATCCAAGAAGATGAGCTGCGGAACCTGCTGGAGAAGAAGCCTGAACCACTGTGCTATGACGGCTTCGAACCCTCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGGTGCAGAGTGAAAATATGGATTGCAGATTGGTTTGCTCAGCTAAACAATAAAATGGGGGGCGATCTGAAGAAAATTGAGACTGTTGGCCAGTACCTGATCGAGATATGGAAAGCAGTGGGGATGGATCTGGAAGGAGGCAAGGTCGAATTTTTGTGGTCGTCCAAGGAGATTAACTCAAGGGCATCAGAATACTGGCCCCTTGTAATGGATATTGCTCGGAGGAACAAGCTTCCAAGGATAATTAGGTGTAGCCAAATTATGGGAAGAAATGAGCAGGATGAGTTGAGCGCCGCCCAAATTTTCTATCCATGCATGCAATGTGCCGACATTTTTTTCCTGAAGGCTGATATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTACTTGCAAGAGAGTATTGTGATGATATCAAGAGGAAGAACAAGCCTATCATCCTATCACACCACATGTTACCGGGCTTACAGCAAGGGCAGGAGAAGATGTCAAAAAGTGATCCGTCATCCTCTATATTTATGGAAGATGATGAGGCGGAAGTGAATGTGAAGATAAAGAAAGCATACTGCCCTCCATTGATCGTGGAAGGGAATCCATGCCTGGAGTACATTAAGTATCTTATTTTCCCTTGGTTCAAGGAGTTCACGGTGGAACGAAGTGCTGATCATGGTGGAAATAAGACCTTCACAAGCTTCGAAGAACTGGTTGCTGACTACGAAACAGGAGTACTGCATCCAGGTGATCTCAAACCAGCATTGTCTAAGGCTTTGAATAAGATACTGGAGCCTGTACGAGAGCACTTCAAAAATGATAGCAGAGCCAAAGATTTATTAAAGAGGGTCAAGGTATGTGTTTGGCTTAAT

Coding sequence (CDS)

ATGGCGACGGAAGCGCCTTCACAGGCCCCATCCGAGGATATACAGTCCCTCTCAATCTCCGACGCTTCAGTGCAAGAGCCCGGTTGCTCATCGAACTCTTCATCAAACCAATTGAGCTTGGACGAGAAGTTTCGAATTGTTAGAAGTGTTGGGGAAGAGTGTATCCAAGAAGATGAGCTGCGGAACCTGCTGGAGAAGAAGCCTGAACCACTGTGCTATGACGGCTTCGAACCCTCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGGTGCAGAGTGAAAATATGGATTGCAGATTGGTTTGCTCAGCTAAACAATAAAATGGGGGGCGATCTGAAGAAAATTGAGACTGTTGGCCAGTACCTGATCGAGATATGGAAAGCAGTGGGGATGGATCTGGAAGGAGGCAAGGTCGAATTTTTGTGGTCGTCCAAGGAGATTAACTCAAGGGCATCAGAATACTGGCCCCTTGTAATGGATATTGCTCGGAGGAACAAGCTTCCAAGGATAATTAGGTGTAGCCAAATTATGGGAAGAAATGAGCAGGATGAGTTGAGCGCCGCCCAAATTTTCTATCCATGCATGCAATGTGCCGACATTTTTTTCCTGAAGGCTGATATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTACTTGCAAGAGAGTATTGTGATGATATCAAGAGGAAGAACAAGCCTATCATCCTATCACACCACATGTTACCGGGCTTACAGCAAGGGCAGGAGAAGATGTCAAAAAGTGATCCGTCATCCTCTATATTTATGGAAGATGATGAGGCGGAAGTGAATGTGAAGATAAAGAAAGCATACTGCCCTCCATTGATCGTGGAAGGGAATCCATGCCTGGAGTACATTAAGTATCTTATTTTCCCTTGGTTCAAGGAGTTCACGGTGGAACGAAGTGCTGATCATGGTGGAAATAAGACCTTCACAAGCTTCGAAGAACTGGTTGCTGACTACGAAACAGGAGTACTGCATCCAGGTGATCTCAAACCAGCATTGTCTAAGGCTTTGAATAAGATACTGGAGCCTGTACGAGAGCACTTCAAAAATGATAGCAGAGCCAAAGATTTATTAAAGAGGGTCAAGGTATGTGTTTGGCTTAAT

Protein sequence

MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDELRNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVREHFKNDSRAKDLLKRVKVCVWLN

Homology

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BLAST of MS017712 vs. NCBI nr
Match: XP_022159739.1 (tyrosine--tRNA ligase 1, cytoplasmic [Momordica charantia])

HSP 1 Score: 773.1 bits (1995), Expect = 1.1e-219
Identity = 380/382 (99.48%), Postives = 381/382 (99.74%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL
Sbjct: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLLEKKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLEKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVGQYLIEIWKAVGMDLE GKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR
Sbjct: 121 GDLKKIETVGQYLIEIWKAVGMDLERGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVREHFKNDSRAKDLLKRVK
Sbjct: 361 LEPVREHFKNDSRAKDLLKRVK 382

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BLAST of MS017712 vs. NCBI nr
Match: KAA0042754.1 (tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 744.2 bits (1920), Expect = 5.7e-211
Identity = 362/385 (94.03%), Postives = 375/385 (97.40%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPSED+QSLSISDA VQEP CSSN SSNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSN-SSNQLSLEEKYQIVRSVGEECIQEDEL 102

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 103 RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 162

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIARRNKLPR
Sbjct: 163 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPR 222

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 223 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 282

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVE NPC
Sbjct: 283 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPC 342

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTFT+FEELV+DY  G LHPGDLKPALSKALNKI
Sbjct: 343 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKI 402

Query: 361 LEPVREHFKNDSRAKDLLKRVKVCV 386
           LEPVR+HFKNDS AKDLLKRVKVCV
Sbjct: 403 LEPVRDHFKNDSNAKDLLKRVKVCV 426

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BLAST of MS017712 vs. NCBI nr
Match: XP_008437283.1 (PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo])

HSP 1 Score: 737.3 bits (1902), Expect = 7.0e-209
Identity = 359/382 (93.98%), Postives = 372/382 (97.38%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPSED+QSLSISDA VQEP CSSN SSNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSN-SSNQLSLEEKYQIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIARRNKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVE NPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTFT+FEELV+DY  G LHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVR+HFKNDS AKDLLKRVK
Sbjct: 361 LEPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of MS017712 vs. NCBI nr
Match: XP_004143904.1 (tyrosine--tRNA ligase 1, cytoplasmic [Cucumis sativus] >KGN50094.1 hypothetical protein Csa_000293 [Cucumis sativus])

HSP 1 Score: 736.5 bits (1900), Expect = 1.2e-208
Identity = 357/382 (93.46%), Postives = 373/382 (97.64%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAP+QAPSED+QSLS+SDA VQEP CSSN SSNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSN-SSNQLSLEEKYQIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIARRNKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTF++FEELV+DY  G LHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVR+HFKNDS AKDLLKRVK
Sbjct: 361 LEPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of MS017712 vs. NCBI nr
Match: XP_038906448.1 (tyrosine--tRNA ligase 1, cytoplasmic [Benincasa hispida])

HSP 1 Score: 735.7 bits (1898), Expect = 2.0e-208
Identity = 359/382 (93.98%), Postives = 372/382 (97.38%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQA SED+QSLSISDA VQEP CSSN  SNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 1   MATEAPSQALSEDLQSLSISDAKVQEPACSSN-FSNQLSLEEKYQIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMK INVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA+EYWPLVMDIAR+NKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAAEYWPLVMDIARKNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTF +FEELVADY TGVLHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFATFEELVADYATGVLHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVREHFKNDS AKDLLKRVK
Sbjct: 361 LEPVREHFKNDSNAKDLLKRVK 381

[Close]

BLAST of MS017712 vs. ExPASy Swiss-Prot
Match: Q8S9J2 (Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g33840 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 5.1e-170
Identity = 287/376 (76.33%), Postives = 329/376 (87.50%), Query Frame = 0


Query: 11  SEDIQSLS--ISDASVQEPGCSSNSSSNQLS--LDEKFRIVRSVGEECIQEDELRNLLEK 70
           SE + +LS  +   SV     SS+S   Q+S  ++++++IVRS+GEECIQE+EL+NLL K
Sbjct: 6   SEIVNALSSEMEAVSVSSTQASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELKNLLAK 65

Query: 71  KPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKI 130
           K  P+CYDGFEPSGRMHIAQGVMK INVNK+TSAGCRVKIWIADWFAQLNNKMGGDLKKI
Sbjct: 66  KAAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGGDLKKI 125

Query: 131 ETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPRIIRCSQ 190
             VG+Y  EIWKA GMD    KVEFLWSS+EINS+A +YWPLVMDIAR+NKLPRI+RC Q
Sbjct: 126 RVVGEYFQEIWKAAGMD--NDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRILRCVQ 185

Query: 191 IMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPI 250
           IMGR+E DELSAAQI YPCMQCADIFFL+ADICQLGMDQRKVNVLAREYCDDIKRKNKPI
Sbjct: 186 IMGRSETDELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKRKNKPI 245

Query: 251 ILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKY 310
           ILSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVNVKIKKAYCPP +V+GNPCLEYIKY
Sbjct: 246 ILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCLEYIKY 305

Query: 311 LIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVRE 370
           +I PWF EFTVER+ ++GGNKT+ SFE++ ADYE+G LHPGDLK  L  ALNKIL+PVR+
Sbjct: 306 IILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKILQPVRD 365

Query: 371 HFKNDSRAKDLLKRVK 383
           HFK D+RAK+LLK++K
Sbjct: 366 HFKTDARAKNLLKQIK 379

[Close]

BLAST of MS017712 vs. ExPASy Swiss-Prot
Match: F4HWL4 (Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g28350 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-155
Identity = 266/357 (74.51%), Postives = 305/357 (85.43%), Query Frame = 0


Query: 30  SSNSSSNQLSLDE----KFRIVRSVGEECIQEDELRNLLEKKPEPLCYDGFEPSGRMHIA 89
           S++SS+  L + E    K++IVRS+GEECIQEDEL+NLL KKP P+CYDGFEPSGRMHIA
Sbjct: 389 SASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPSGRMHIA 448

Query: 90  QGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGQYLIEIWKAVGMDLE 149
           QGVMK  NVNKLTSAGC+VKIWIADWFAQLNNK+GGDL++I  VG+Y  EIW+A GM+  
Sbjct: 449 QGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQAGGMN-- 508

Query: 150 GGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELSAAQIFYPC 209
             KVEFLW+S EIN + S+YWPLVMDIARRN L RI+RC QIMGR+E + LSAAQI YPC
Sbjct: 509 NDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAAQILYPC 568

Query: 210 MQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSK 269
           MQCADIF L+ADICQLGMDQRKVN+LAREYC DIKRKNKPIILSHHMLPGLQQGQEKMSK
Sbjct: 569 MQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLPGLQQGQEKMSK 628

Query: 270 SDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERSADHGG 329
           SDPSS+IFMED+EA+VN KI KAYCPP  VEGNPCLEY+KY++ P F EF VE S  +GG
Sbjct: 629 SDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNEFKVE-SEKNGG 688

Query: 330 NKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVREHFKNDSRAKDLLKRVK 383
           NKTF SFE++VADYETG LHP DLK AL KALN  L+PVR+HFK + RAK+LL++VK
Sbjct: 689 NKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQPVRDHFKTNERAKNLLEQVK 742

[Close]

BLAST of MS017712 vs. ExPASy Swiss-Prot
Match: Q5UPJ7 (Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.5e-73
Identity = 153/337 (45.40%), Postives = 212/337 (62.91%), Query Frame = 0


Query: 49  SVGEECIQEDELRNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWI 108
           S+ EEC   D L+ L++       Y+GFEPSGR+HIAQ ++  +N N +   G ++ I+I
Sbjct: 18  SIAEECETLDRLKQLVDSGRIFTAYNGFEPSGRIHIAQALITVMNTNNIIECGGQMIIYI 77

Query: 109 ADWFAQLNNKMGGDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPL 168
           ADWFA++N KM GD+ KI  +G+Y IE++KA G++L+G    F+W+S+ I S  S Y   
Sbjct: 78  ADWFAKMNLKMNGDINKIRELGRYFIEVFKACGINLDG--TRFIWASEFIASNPS-YIER 137

Query: 169 VMDIARRNKLPRIIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFL---KADICQLGMDQ 228
           ++DIA  + + R+ RC QIMGRNE D L A+QIFYPCMQ AD+F L     DICQLG+DQ
Sbjct: 138 MLDIAEFSTISRVKRCCQIMGRNESDCLKASQIFYPCMQAADVFELVPEGIDICQLGIDQ 197

Query: 229 RKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKI 288
           RKVN+LA EY +D +    PI LSHHML  L   ++KMSKSDP  +IFM+D E EV+ KI
Sbjct: 198 RKVNMLAIEYAND-RGLKIPISLSHHMLMSLSGPKKKMSKSDPQGAIFMDDTEQEVSEKI 257

Query: 289 KKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLH 348
            +AYC     + NP  EYIKYL+  WF    +         K +T  E +  D+ +  ++
Sbjct: 258 SRAYCTDETFD-NPIFEYIKYLLLRWFGTLNL-------CGKIYTDIESIQEDFSS--MN 317

Query: 349 PGDLKPALSKALNKILEPVREHFKNDSRAKDLLKRVK 383
             +LK  ++  +N I++ VREHFK      +LL  VK
Sbjct: 318 KRELKTDVANYINTIIDLVREHFKK-PELSELLSNVK 339

[Close]

BLAST of MS017712 vs. ExPASy Swiss-Prot
Match: Q5JF63 (Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=tyrS PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 7.1e-63
Identity = 136/375 (36.27%), Postives = 214/375 (57.07%), Query Frame = 0


Query: 38  LSLDEKFRIV-RSVGEECIQEDELRNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNK 97
           + ++ K  ++ +   EE + E+ LR+LLE       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIERKIELIKKKPTEELLTEENLRHLLEVGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 98  LTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGQYLIEIWKAVGMDLEGG---KVEFLW 157
           L  AG + +I++ADW + +N+K+GGDL+ I+ V     +      + + GG   KVEF+ 
Sbjct: 61  LQKAGVKTRIFLADWHSWINDKLGGDLEVIQKVALTYFKEGMKQSIKVMGGDPDKVEFVL 120

Query: 158 SSKEINSRASEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELSAAQIFYPCMQCADIFF 217
           +S+ ++    +YW  V+DI++   L R++R   IMGR   + +  A++ YP MQ ADIF+
Sbjct: 121 ASEILDK--GDYWQTVIDISKNVTLARMLRSITIMGRQMGEAIDFAKLIYPAMQVADIFY 180

Query: 218 LKADICQLGMDQRKVNVLAREYCDDIK--------RKNKPIILSHHMLPGLQQ------- 277
               I   GMDQRK +V+A E    +K         K KP+ L HH+L GLQ+       
Sbjct: 181 QGVTIAHAGMDQRKAHVIAIEVAQKLKYHPLEWKGEKLKPVALHHHLLLGLQEPPVWPIE 240

Query: 278 ---------GQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKYLIF 337
                     Q KMSKS P S++F+ D   E+  K++KA+CP   V+ NP L++ +Y+IF
Sbjct: 241 SEEQFKELKTQMKMSKSKPYSAVFIHDSPEEIKQKLRKAFCPAREVKYNPVLDWAEYIIF 300

Query: 338 -PWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVREHF 384
                EFT+ R A  GG+ T+T+FEEL  D+  G LHP DLK A+++ L ++L+PVRE+F
Sbjct: 301 REEPTEFTIHRPAKFGGDVTYTTFEELKKDFAEGKLHPLDLKNAVAEYLIELLKPVREYF 360

[Close]

BLAST of MS017712 vs. ExPASy Swiss-Prot
Match: Q8U2H3 (Tyrosine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=tyrS PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.6e-62
Identity = 130/375 (34.67%), Postives = 216/375 (57.60%), Query Frame = 0


Query: 38  LSLDEKFRIV-RSVGEECIQEDELRNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNK 97
           + ++E+ ++V +   EE +  + LR+L E       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIEERIKLVLKKPTEEVLTVENLRHLFEIGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 98  LTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGQYLIEIWKAVGMDLEGG---KVEFLW 157
              AG + ++++ADW + +N+K+GGDL+ I+ V     ++     +++ GG   KVEF+ 
Sbjct: 61  FQKAGIKTRVFLADWHSWINDKLGGDLETIQEVALKYFKVGMEKSIEVMGGKPEKVEFVL 120

Query: 158 SSKEINSRASEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELSAAQIFYPCMQCADIFF 217
           +S+ +     +YW  V+DI++   L R++R   IMGR   + +  A++ YP MQ ADIF+
Sbjct: 121 ASEILEK--GDYWQTVIDISKNVTLSRVLRSITIMGRKMGEAIDFAKLIYPMMQVADIFY 180

Query: 218 LKADICQLGMDQRKVNVLAREYCDDIK--------RKNKPIILSHHMLPGLQQ------- 277
               I   GMDQRK +V+A E  + ++         K KP+ + HH+L GLQ+       
Sbjct: 181 QGVTIAHAGMDQRKAHVIAIEVAEKLRYHPIIHNGEKLKPVAVHHHLLLGLQEPPKWPIE 240

Query: 278 ---------GQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKYLIF 337
                     + KMSKS P S++F+ D   E+  K++KA+CP   V+ NP L++++YL+F
Sbjct: 241 SEEEFKEIKAEMKMSKSKPYSAVFIHDSPEEIREKLRKAFCPAREVKYNPVLDWVEYLVF 300

Query: 338 -PWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVREHF 384
                EFT+ R A +GG+ T+T+FEEL  D+  G LHP DLK A+++ L  +LEP+R++F
Sbjct: 301 REEPTEFTIHRPAKYGGDVTYTTFEELKRDFAEGKLHPLDLKNAVAEYLIDLLEPIRKYF 360

[Close]

BLAST of MS017712 vs. ExPASy TrEMBL
Match: A0A6J1E377 (Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111026078 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 5.6e-220
Identity = 380/382 (99.48%), Postives = 381/382 (99.74%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL
Sbjct: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLLEKKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLEKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVGQYLIEIWKAVGMDLE GKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR
Sbjct: 121 GDLKKIETVGQYLIEIWKAVGMDLERGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVREHFKNDSRAKDLLKRVK
Sbjct: 361 LEPVREHFKNDSRAKDLLKRVK 382

[Close]

BLAST of MS017712 vs. ExPASy TrEMBL
Match: A0A5A7THE1 (Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002540 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 2.8e-211
Identity = 362/385 (94.03%), Postives = 375/385 (97.40%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPSED+QSLSISDA VQEP CSSN SSNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSN-SSNQLSLEEKYQIVRSVGEECIQEDEL 102

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 103 RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 162

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIARRNKLPR
Sbjct: 163 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPR 222

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 223 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 282

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVE NPC
Sbjct: 283 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPC 342

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTFT+FEELV+DY  G LHPGDLKPALSKALNKI
Sbjct: 343 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKI 402

Query: 361 LEPVREHFKNDSRAKDLLKRVKVCV 386
           LEPVR+HFKNDS AKDLLKRVKVCV
Sbjct: 403 LEPVRDHFKNDSNAKDLLKRVKVCV 426

[Close]

BLAST of MS017712 vs. ExPASy TrEMBL
Match: A0A1S3ATS8 (Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.4e-209
Identity = 359/382 (93.98%), Postives = 372/382 (97.38%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPSED+QSLSISDA VQEP CSSN SSNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSN-SSNQLSLEEKYQIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIARRNKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVE NPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTFT+FEELV+DY  G LHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVR+HFKNDS AKDLLKRVK
Sbjct: 361 LEPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of MS017712 vs. ExPASy TrEMBL
Match: A0A0A0KMK0 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 5.8e-209
Identity = 357/382 (93.46%), Postives = 373/382 (97.64%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAP+QAPSED+QSLS+SDA VQEP CSSN SSNQLSL+EK++IVRSVGEECIQEDEL
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSN-SSNQLSLEEKYQIVRSVGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLL+KKPEP+CYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIARRNKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRNEQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTF++FEELV+DY  G LHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVR+HFKNDS AKDLLKRVK
Sbjct: 361 LEPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of MS017712 vs. ExPASy TrEMBL
Match: A0A6J1ETE5 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 2.9e-208
Identity = 356/382 (93.19%), Postives = 375/382 (98.17%), Query Frame = 0


Query: 1   MATEAPSQAPSEDIQSLSISDASVQEPGCSSNSSSNQLSLDEKFRIVRSVGEECIQEDEL 60
           MATEAPSQAPS+D+QSLSI++A VQEPG SS+SSSNQLSL+EKF+IVRS+GEECIQEDEL
Sbjct: 1   MATEAPSQAPSQDLQSLSIAEAKVQEPG-SSSSSSNQLSLEEKFKIVRSIGEECIQEDEL 60

Query: 61  RNLLEKKPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMG 120
           RNLLEKKPEP+CYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPR 180
           GDLKKIETVG+YLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIAR+NKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPR 180

Query: 181 IIRCSQIMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGR EQDEL+AAQI YPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVN+KIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVER+ADHGGNKTFT+FEELVADY +GVLHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKI 360

Query: 361 LEPVREHFKNDSRAKDLLKRVK 383
           LEPVR+HFKNDS AKDLLKRVK
Sbjct: 361 LEPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of MS017712 vs. TAIR 10
Match: AT2G33840.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 598.6 bits (1542), Expect = 3.6e-171
Identity = 287/376 (76.33%), Postives = 329/376 (87.50%), Query Frame = 0


Query: 11  SEDIQSLS--ISDASVQEPGCSSNSSSNQLS--LDEKFRIVRSVGEECIQEDELRNLLEK 70
           SE + +LS  +   SV     SS+S   Q+S  ++++++IVRS+GEECIQE+EL+NLL K
Sbjct: 6   SEIVNALSSEMEAVSVSSTQASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELKNLLAK 65

Query: 71  KPEPLCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKI 130
           K  P+CYDGFEPSGRMHIAQGVMK INVNK+TSAGCRVKIWIADWFAQLNNKMGGDLKKI
Sbjct: 66  KAAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGGDLKKI 125

Query: 131 ETVGQYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPRIIRCSQ 190
             VG+Y  EIWKA GMD    KVEFLWSS+EINS+A +YWPLVMDIAR+NKLPRI+RC Q
Sbjct: 126 RVVGEYFQEIWKAAGMD--NDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRILRCVQ 185

Query: 191 IMGRNEQDELSAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPI 250
           IMGR+E DELSAAQI YPCMQCADIFFL+ADICQLGMDQRKVNVLAREYCDDIKRKNKPI
Sbjct: 186 IMGRSETDELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKRKNKPI 245

Query: 251 ILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKY 310
           ILSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVNVKIKKAYCPP +V+GNPCLEYIKY
Sbjct: 246 ILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCLEYIKY 305

Query: 311 LIFPWFKEFTVERSADHGGNKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVRE 370
           +I PWF EFTVER+ ++GGNKT+ SFE++ ADYE+G LHPGDLK  L  ALNKIL+PVR+
Sbjct: 306 IILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKILQPVRD 365

Query: 371 HFKNDSRAKDLLKRVK 383
           HFK D+RAK+LLK++K
Sbjct: 366 HFKTDARAKNLLKQIK 379

[Close]

BLAST of MS017712 vs. TAIR 10
Match: AT1G28350.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 550.8 bits (1418), Expect = 8.6e-157
Identity = 266/357 (74.51%), Postives = 305/357 (85.43%), Query Frame = 0


Query: 30  SSNSSSNQLSLDE----KFRIVRSVGEECIQEDELRNLLEKKPEPLCYDGFEPSGRMHIA 89
           S++SS+  L + E    K++IVRS+GEECIQEDEL+NLL KKP P+CYDGFEPSGRMHIA
Sbjct: 389 SASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPSGRMHIA 448

Query: 90  QGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGQYLIEIWKAVGMDLE 149
           QGVMK  NVNKLTSAGC+VKIWIADWFAQLNNK+GGDL++I  VG+Y  EIW+A GM+  
Sbjct: 449 QGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQAGGMN-- 508

Query: 150 GGKVEFLWSSKEINSRASEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELSAAQIFYPC 209
             KVEFLW+S EIN + S+YWPLVMDIARRN L RI+RC QIMGR+E + LSAAQI YPC
Sbjct: 509 NDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAAQILYPC 568

Query: 210 MQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSK 269
           MQCADIF L+ADICQLGMDQRKVN+LAREYC DIKRKNKPIILSHHMLPGLQQGQEKMSK
Sbjct: 569 MQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLPGLQQGQEKMSK 628

Query: 270 SDPSSSIFMEDDEAEVNVKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERSADHGG 329
           SDPSS+IFMED+EA+VN KI KAYCPP  VEGNPCLEY+KY++ P F EF VE S  +GG
Sbjct: 629 SDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNEFKVE-SEKNGG 688

Query: 330 NKTFTSFEELVADYETGVLHPGDLKPALSKALNKILEPVREHFKNDSRAKDLLKRVK 383
           NKTF SFE++VADYETG LHP DLK AL KALN  L+PVR+HFK + RAK+LL++VK
Sbjct: 689 NKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQPVRDHFKTNERAKNLLEQVK 742

The following BLAST results are available for this feature:

BLAST of MS017712 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159739.1	1.1e-219	99.48	tyrosine--tRNA ligase 1, cytoplasmic [Momordica charantia]	[more]
KAA0042754.1	5.7e-211	94.03	tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa]	[more]
XP_008437283.1	7.0e-209	93.98	PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo]	[more]
XP_004143904.1	1.2e-208	93.46	tyrosine--tRNA ligase 1, cytoplasmic [Cucumis sativus] >KGN50094.1 hypothetical ...	[more]
XP_038906448.1	2.0e-208	93.98	tyrosine--tRNA ligase 1, cytoplasmic [Benincasa hispida]	[more]

BLAST of MS017712 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S9J2	5.1e-170	76.33	Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g3384...	[more]
F4HWL4	1.2e-155	74.51	Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g2835...	[more]
Q5UPJ7	1.5e-73	45.40	Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1...	[more]
Q5JF63	7.1e-63	36.27	Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12...	[more]
Q8U2H3	1.6e-62	34.67	Tyrosine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM...	[more]

BLAST of MS017712 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E377	5.6e-220	99.48	Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111026078 PE=3 SV=1	[more]
A0A5A7THE1	2.8e-211	94.03	Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3ATS8	3.4e-209	93.98	Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1	[more]
A0A0A0KMK0	5.8e-209	93.46	Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1	[more]
A0A6J1ETE5	2.9e-208	93.19	Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1	[more]

BLAST of MS017712 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33840.1	3.6e-171	76.33	Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial	[more]
AT1G28350.1	8.6e-157	74.51	Nucleotidylyl transferase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 196..385 e-value: 6.3E-81 score: 273.8
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 31..195 e-value: 6.1E-69 score: 234.4
IPR023617	Tyrosine-tRNA ligase, archaeal/eukaryotic-type	PIRSF	PIRSF006588	TyrRS_arch_euk	coord: 37..385 e-value: 3.0E-145 score: 481.5
IPR002305	Aminoacyl-tRNA synthetase, class Ic	PFAM	PF00579	tRNA-synt_1b	coord: 69..362 e-value: 1.8E-66 score: 224.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	PANTHER	PTHR46264:SF8	BNAANNG11300D PROTEIN	coord: 23..385
None	No IPR available	PANTHER	PTHR46264	TYROSINE-TRNA LIGASE	coord: 23..385
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 45..372

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017712.1	MS017712.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006418	tRNA aminoacylation for protein translation
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0004831	tyrosine-tRNA ligase activity