MS017319 (gene) Bitter gourd (TR) v1

Overview


Name	MS017319
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	glutathione S-transferase DHAR2-like
Location	scaffold33: 1575296 .. 1577134 (-)
RNA-Seq Expression	MS017319
Synteny	MS017319

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTCGAGGTTGCTGCCAAGGCTGCCGTTGGTGCACCCGATGTTATCGGAGACTGTATGTTCTTCTTTCCCACCGGAAATTTGTTTGAGTTTCTCTTAATTTTGACTTTGGGGTTTTTTTTCTCTCTCTCTCCTGCAGGCCCTTTCACCCAGAGGGTTCTCTTAACTTTGGAGGAGAAGAAAATTCCTTACAAACTGCATCTAATCAATCTCTCCGACAAACCCAGTTGGTAATATCTCGAAATCTTGCTTCTATTTCAGTTTCATTTCGGGTTGTGCTACTTTAAGAGGGTTTTTAAGCAAATGGGGTTGTGTGGATTGTTAATGATTTTGGCAGGTTCTTGGAAGTGAGCCCAGAAGGGAAAGTTCCGGTGGTGAAGTTTGATGACAAATGGGTTCCTGACTCCGATGTAATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTCGTTACTCCTCCTGAATTTTCTTCTGTGTAAGCTCCTTTACCTCCTGAATTTTTCTATTCACTGTTTGCTGTTCTTCTTCGTTGATCAATGTTTTCAACAGTTCAGACTTGAAGTTTCTATTTCTTGAGTTGTGTGACTATTACTGTGGAGTTGGGATGATTGCATGAATTGTAACCACATGTCTTTGTGTTTGTTTGATGTGTCCATTGTTTTCAAGTCTCACTGACTTCTAGTTTGGCAAGATGTTTGGTTCTGTTGTAGCTAAGTTTGTCTTGTGATGGTGGCTGAATATTGATTGTATTGCATTTAAGGCATTAGATCTAGATGAAGACAACTTGATCTCTTTACTGGTTTGGATAAAGAATTAATTTCATAATTATTCCTTCTACGAGTTGTACAAACTTCTTGTTTCTCTACCTTTTAATCCACGGAGAAAGTGTCTTAATTTGGTCTAGTTCAGTACTCTCGTTAGGCATCATCCAAGATTGTAATTGCCTCGGCTTTCCTCAAATTATAAACTATGCTTTGGTTCTGAATAAAACTGTCCTCTCTGTACTTTGGTTCATAGGGGATCAAAGATAAACGGTGCTTTTGTAAAATTCTTGATGAGCAAAGACTCGAATGATGGTTCGGAGCAGGCATTGCTTGAAGAGTTGAAGGCGTTGGACAAGCATCTTAAGGCTCATGTATGTGCGCCGTGCATAATGTATCGAACAGCGATTTTAAAGTGTTTATGATTTTGCTCTCCTCATTTTTGGCAATGTCTCATGGTGCTAGGGTCCTTATGTTGCTGGAGAGAAGGTAACTGCTGCTGATTTGAGTCTGGCACCAAAGTTGTACCAAATTGATGTTGCTCTTGGCCACTTCAAGAAGTGGACTTTCCCTGAAAACTTAGCCAGTTTGTCTGCCTACAAAAAGGTACGATGTAGTTGCACCGACGTTGTATAGCTTGCAGTTGCATCTTTTAGTTCAATCAGATCTTTTTAAGCCAGTATGAGGTTTCTATTTGTTATCAGTTTCATTTTCGTTGCTCTCTATCTGCTTAGACTTTGCTGGCTTCTTAGATTACTTGCAGTTTGAAATCTAATGTGCTGGTTGGTTTCTGCTCAACTAAACTCGAATCTCCGATAACGGGGTTAACTGATGGAGAATGCCAGGATAAAATTTGTTCTTTTTTAAGTGTAGATGGGTTCATCGTCACCCCGACGAGTGGGACATGAGTTCATCTGGTTAGGAGACGAATCAGGATCCATATTTGTTCAATGACAGTGAAGGGTGAATGTTGATTTTGCAGTTGCTTTTCGCTCGAGAATCCTTCGTGAAGACAAAGGCTGCCCCGGAACATGTAATTGCTGGGTGGGCGCTGAAGGTGAATGCTGCT

mRNA sequence

ATGGCCCTCGAGGTTGCTGCCAAGGCTGCCGTTGGTGCACCCGATGTTATCGGAGACTGTATGTTCTTCTTTCCCACCGGAAATTTGTTTGAGTTTCTCTTAATTTTGACTTTGGGGTTTTTTTTCTCTCTCTCTCCTGCAGGCCCTTTCACCCAGAGGGTTCTCTTAACTTTGGAGGAGAAGAAAATTCCTTACAAACTGCATCTAATCAATCTCTCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCAGAAGGGAAAGTTCCGGTGGTGAAGTTTGATGACAAATGGGTTCCTGACTCCGATGTAATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTCGTTACTCCTCCTGAATTTTCTTCTGTGGGATCAAAGATAAACGGTGCTTTTGTAAAATTCTTGATGAGCAAAGACTCGAATGATGGTTCGGAGCAGGCATTGCTTGAAGAGTTGAAGGCGTTGGACAAGCATCTTAAGGCTCATGGTCCTTATGTTGCTGGAGAGAAGGTAACTGCTGCTGATTTGAGTCTGGCACCAAAGTTGTACCAAATTGATGTTGCTCTTGGCCACTTCAAGAAGTGGACTTTCCCTGAAAACTTAGCCAGTTTGTCTGCCTACAAAAAGTTGCTTTTCGCTCGAGAATCCTTCGTGAAGACAAAGGCTGCCCCGGAACATGTAATTGCTGGGTGGGCGCTGAAGGTGAATGCTGCT

Coding sequence (CDS)

ATGGCCCTCGAGGTTGCTGCCAAGGCTGCCGTTGGTGCACCCGATGTTATCGGAGACTGTATGTTCTTCTTTCCCACCGGAAATTTGTTTGAGTTTCTCTTAATTTTGACTTTGGGGTTTTTTTTCTCTCTCTCTCCTGCAGGCCCTTTCACCCAGAGGGTTCTCTTAACTTTGGAGGAGAAGAAAATTCCTTACAAACTGCATCTAATCAATCTCTCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCAGAAGGGAAAGTTCCGGTGGTGAAGTTTGATGACAAATGGGTTCCTGACTCCGATGTAATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTCGTTACTCCTCCTGAATTTTCTTCTGTGGGATCAAAGATAAACGGTGCTTTTGTAAAATTCTTGATGAGCAAAGACTCGAATGATGGTTCGGAGCAGGCATTGCTTGAAGAGTTGAAGGCGTTGGACAAGCATCTTAAGGCTCATGGTCCTTATGTTGCTGGAGAGAAGGTAACTGCTGCTGATTTGAGTCTGGCACCAAAGTTGTACCAAATTGATGTTGCTCTTGGCCACTTCAAGAAGTGGACTTTCCCTGAAAACTTAGCCAGTTTGTCTGCCTACAAAAAGTTGCTTTTCGCTCGAGAATCCTTCGTGAAGACAAAGGCTGCCCCGGAACATGTAATTGCTGGGTGGGCGCTGAAGGTGAATGCTGCT

Protein sequence

MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEEKKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLSLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNAA

Homology

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BLAST of MS017319 vs. NCBI nr
Match: XP_022139338.1 (glutathione S-transferase DHAR2-like [Momordica charantia])

HSP 1 Score: 407.1 bits (1045), Expect = 1.0e-109
Identity = 211/241 (87.55%), Postives = 212/241 (87.97%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           M LEVAAKAAVGAPDVIGDC                            PFTQRVLLTLEE
Sbjct: 1   MDLEVAAKAAVGAPDVIGDC----------------------------PFTQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALD+HLKAHGPYVAGEKVTAADLS
Sbjct: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDEHLKAHGPYVAGEKVTAADLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA
Sbjct: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

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BLAST of MS017319 vs. NCBI nr
Match: XP_022985936.1 (uncharacterized protein LOC111483829 isoform X1 [Cucurbita maxima])

HSP 1 Score: 369.0 bits (946), Expect = 3.1e-98
Identity = 188/241 (78.01%), Postives = 199/241 (82.57%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EVA KAA+GAP+VIGDC                            PF+QRVLLTLEE
Sbjct: 1   MAIEVAVKAAIGAPNVIGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYKLHLINLSDKP WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKVPYKLHLINLSDKPIWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVK L SKD NDGSE ALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY +D+ALGHFKKW FP NL SLS YK+LLFARESF+KTKAAPEHVI+GW  KVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

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BLAST of MS017319 vs. NCBI nr
Match: XP_022985944.1 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 369.0 bits (946), Expect = 3.1e-98
Identity = 188/241 (78.01%), Postives = 199/241 (82.57%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EVA KAA+GAP+VIGDC                            PF+QRVLLTLEE
Sbjct: 1   MAIEVAVKAAIGAPNVIGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYKLHLINLSDKP WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKVPYKLHLINLSDKPIWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVK L SKD NDGSE ALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY +D+ALGHFKKW FP NL SLS YK+LLFARESF+KTKAAPEHVI+GW  KVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

[Close]

BLAST of MS017319 vs. NCBI nr
Match: XP_022985954.1 (uncharacterized protein LOC111483829 isoform X3 [Cucurbita maxima])

HSP 1 Score: 369.0 bits (946), Expect = 3.1e-98
Identity = 188/241 (78.01%), Postives = 199/241 (82.57%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EVA KAA+GAP+VIGDC                            PF+QRVLLTLEE
Sbjct: 1   MAIEVAVKAAIGAPNVIGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYKLHLINLSDKP WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKVPYKLHLINLSDKPIWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVK L SKD NDGSE ALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY +D+ALGHFKKW FP NL SLS YK+LLFARESF+KTKAAPEHVI+GW  KVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

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BLAST of MS017319 vs. NCBI nr
Match: XP_022976477.1 (glutathione S-transferase DHAR2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 368.2 bits (944), Expect = 5.3e-98
Identity = 187/241 (77.59%), Postives = 197/241 (81.74%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EV  KAA GAPDV+GDC                            PF  RVLLTLEE
Sbjct: 1   MAIEVVVKAATGAPDVLGDC----------------------------PFCHRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+ YKL L+NLSDKPSWFLEVSPEGKVPVVKFD KWVPDSDVIVGILEEKYP+PSLVTP
Sbjct: 61  KKVAYKLLLVNLSDKPSWFLEVSPEGKVPVVKFDGKWVPDSDVIVGILEEKYPDPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           P+FSSVGSKINGAFVKFL+SKD  DGSEQALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PQFSSVGSKINGAFVKFLLSKDPTDGSEQALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY ID+ALGHFKKW FPENL+SLSAYKKLLFARESFVKTKA PEHVIAGW  KVNA
Sbjct: 181 LAPKLYHIDIALGHFKKWVFPENLSSLSAYKKLLFARESFVKTKAVPEHVIAGWETKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

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BLAST of MS017319 vs. ExPASy Swiss-Prot
Match: Q9FRL8 (Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.2e-83
Identity = 156/241 (64.73%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MAL++  K AVGAPDV+GDC                            PF+QRVLLTLEE
Sbjct: 1   MALDICVKVAVGAPDVLGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYK HLIN+SDKP WFL++SPEGKVPVVK D KWV DSDVIVG+LEEKYPEPSL TP
Sbjct: 61  KKLPYKTHLINVSDKPQWFLDISPEGKVPVVKLDGKWVADSDVIVGLLEEKYPEPSLKTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAH-GPYVAGEKVTAADL 180
           PEF+SVGSKI GAFV FL SKD+NDGSE+AL++EL+AL+ HLK H GP+VAGEK+TA DL
Sbjct: 121 PEFASVGSKIFGAFVTFLKSKDANDGSEKALVDELEALENHLKTHSGPFVAGEKITAVDL 180

Query: 181 SLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           SLAPKLY ++VALGH+K W+ PE+L S+  Y K LF+RESF  TKA  E V+AGW  KVN
Sbjct: 181 SLAPKLYHLEVALGHYKNWSVPESLTSVRNYAKALFSRESFENTKAKKEIVVAGWESKVN 213

[Close]

BLAST of MS017319 vs. ExPASy Swiss-Prot
Match: Q9FWR4 (Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 4.5e-76
Identity = 145/240 (60.42%), Postives = 172/240 (71.67%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MALE+  KAAVGAPD +GDC                            PF+QR LLTLEE
Sbjct: 1   MALEICVKAAVGAPDHLGDC----------------------------PFSQRALLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           K + YK+HLINLSDKP WFL++SP+GKVPV+K DDKWV DSDVIVGILEEKYP+P L TP
Sbjct: 61  KSLTYKIHLINLSDKPQWFLDISPQGKVPVLKIDDKWVTDSDVIVGILEEKYPDPPLKTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAH-GPYVAGEKVTAADL 180
            EF+SVGS I G F  FL SKDSNDGSE ALL EL+AL+ HLK+H GP++AGE+V+A DL
Sbjct: 121 AEFASVGSNIFGTFGTFLKSKDSNDGSEHALLVELEALENHLKSHDGPFIAGERVSAVDL 180

Query: 181 SLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           SLAPKLY + VALGHFK W+ PE+   +  Y K LF+ +SF KTK   ++VI+GWA KVN
Sbjct: 181 SLAPKLYHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKYVISGWAPKVN 212

[Close]

BLAST of MS017319 vs. ExPASy Swiss-Prot
Match: Q65XA0 (Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 5.9e-76
Identity = 142/241 (58.92%), Postives = 175/241 (72.61%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           M +EV  KAAVG PD +GDC                            PF+QRVLLTLEE
Sbjct: 1   MGVEVCVKAAVGHPDTLGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVK-FDDKWVPDSDVIVGILEEKYPEPSLVT 120
           KK+PY++ LI++ +KP WFL++SPEGKVPV    D KW+PDSDVI  ++EEKYP PSLVT
Sbjct: 61  KKVPYEMKLIDVQNKPDWFLKISPEGKVPVFNGGDGKWIPDSDVITQVIEEKYPTPSLVT 120

Query: 121 PPEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADL 180
           PPE++SVGSKI   F  FL SKD NDGSE+ALL EL+AL++HLKAHGP++ G+ ++AADL
Sbjct: 121 PPEYASVGSKIFSCFTTFLKSKDPNDGSEKALLTELQALEEHLKAHGPFINGQNISAADL 180

Query: 181 SLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           SLAPKLY + VAL HFK W  PE+L ++ AY + LF+RESF+KTKAA EH+IAGWA KVN
Sbjct: 181 SLAPKLYHLQVALEHFKGWKIPEDLTNVHAYTEALFSRESFIKTKAAKEHLIAGWAPKVN 213

[Close]

BLAST of MS017319 vs. ExPASy Swiss-Prot
Match: Q9FG59 (Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR4 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.3e-72
Identity = 144/244 (59.02%), Postives = 176/244 (72.13%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           M +EV  KAA GAPDV+GDC                            PF QR+LLTLE+
Sbjct: 1   MGIEVCVKAASGAPDVLGDC----------------------------PFGQRILLTLED 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKF--DDKWVPDSDVIVGILEEKYPEPSLV 120
           KK+PYK HLI++S KP WFL +SP+GK+P+VKF  D+ WV DSD+IVGI+EEKYPEPSLV
Sbjct: 61  KKLPYKTHLIDVSLKPDWFLAISPKGKLPLVKFDEDENWVADSDLIVGIIEEKYPEPSLV 120

Query: 121 T-PPEFSSVGSKINGAFVKFLMSKD-SNDGSEQALLEELKALDKHLKAH-GPYVAGEKVT 180
           T PPEF+SVGSKI GAFV FL SKD +NDGS+ ALL+EL+ALD HLK H GP+VAG+KVT
Sbjct: 121 TFPPEFASVGSKIIGAFVMFLTSKDHANDGSDMALLDELEALDHHLKTHVGPFVAGDKVT 180

Query: 181 AADLSLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWA 240
             DLSLAPKLY ++  LGHF  W  PE+L ++  Y K+LF+ ESF KTKAA E++IA WA
Sbjct: 181 VVDLSLAPKLYHLETTLGHFMDWCVPESLTNVRDYMKVLFSLESFEKTKAAKEYLIASWA 216

[Close]

BLAST of MS017319 vs. ExPASy Swiss-Prot
Match: Q67UK9 (Probable glutathione S-transferase DHAR2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=DHAR2 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.0e-67
Identity = 126/236 (53.39%), Postives = 165/236 (69.92%), Query Frame = 0


Query: 3   LEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEEKK 62
           LEV AKA++  PD +GDC                            PFTQRVLLT+EEK 
Sbjct: 63  LEVCAKASLTVPDRLGDC----------------------------PFTQRVLLTIEEKH 122

Query: 63  IPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPE 122
           +PY + L++L++KP WFL++SPEGKVP+VK +++WV DSDVI   +EEKYPEPSL TPPE
Sbjct: 123 LPYDIKLVDLANKPDWFLKISPEGKVPIVKLEEQWVADSDVITQAIEEKYPEPSLATPPE 182

Query: 123 FSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLSLA 182
            +SVGSKI   F+ FL SKD NDG+EQALL EL + D +LK +GP++ GE ++AADLSLA
Sbjct: 183 KASVGSKIFSTFIGFLKSKDPNDGTEQALLSELTSFDSYLKDNGPFINGETISAADLSLA 242

Query: 183 PKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKV 239
           PKLY +++ALGH+K W+ P++L+ +  Y K +F+ +SFVKT A  E VIAGW  KV
Sbjct: 243 PKLYHMEIALGHYKNWSVPDSLSHVKKYMKTIFSMDSFVKTIALQEDVIAGWRPKV 270

[Close]

BLAST of MS017319 vs. ExPASy TrEMBL
Match: A0A6J1CFE5 (glutathione S-transferase DHAR2-like OS=Momordica charantia OX=3673 GN=LOC111010278 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 5.0e-110
Identity = 211/241 (87.55%), Postives = 212/241 (87.97%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           M LEVAAKAAVGAPDVIGDC                            PFTQRVLLTLEE
Sbjct: 1   MDLEVAAKAAVGAPDVIGDC----------------------------PFTQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALD+HLKAHGPYVAGEKVTAADLS
Sbjct: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDEHLKAHGPYVAGEKVTAADLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA
Sbjct: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

[Close]

BLAST of MS017319 vs. ExPASy TrEMBL
Match: A0A6J1JER3 (uncharacterized protein LOC111483829 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.5e-98
Identity = 188/241 (78.01%), Postives = 199/241 (82.57%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EVA KAA+GAP+VIGDC                            PF+QRVLLTLEE
Sbjct: 1   MAIEVAVKAAIGAPNVIGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYKLHLINLSDKP WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKVPYKLHLINLSDKPIWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVK L SKD NDGSE ALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY +D+ALGHFKKW FP NL SLS YK+LLFARESF+KTKAAPEHVI+GW  KVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

[Close]

BLAST of MS017319 vs. ExPASy TrEMBL
Match: A0A6J1JEQ3 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.5e-98
Identity = 188/241 (78.01%), Postives = 199/241 (82.57%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EVA KAA+GAP+VIGDC                            PF+QRVLLTLEE
Sbjct: 1   MAIEVAVKAAIGAPNVIGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYKLHLINLSDKP WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKVPYKLHLINLSDKPIWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVK L SKD NDGSE ALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY +D+ALGHFKKW FP NL SLS YK+LLFARESF+KTKAAPEHVI+GW  KVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

[Close]

BLAST of MS017319 vs. ExPASy TrEMBL
Match: A0A6J1J9M2 (uncharacterized protein LOC111483829 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.5e-98
Identity = 188/241 (78.01%), Postives = 199/241 (82.57%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EVA KAA+GAP+VIGDC                            PF+QRVLLTLEE
Sbjct: 1   MAIEVAVKAAIGAPNVIGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYKLHLINLSDKP WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP
Sbjct: 61  KKVPYKLHLINLSDKPIWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           PEFSSVGSKINGAFVK L SKD NDGSE ALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PEFSSVGSKINGAFVKMLKSKDPNDGSELALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY +D+ALGHFKKW FP NL SLS YK+LLFARESF+KTKAAPEHVI+GW  KVNA
Sbjct: 181 LAPKLYNLDIALGHFKKWIFPVNLNSLSKYKELLFARESFMKTKAAPEHVISGWEPKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

[Close]

BLAST of MS017319 vs. ExPASy TrEMBL
Match: A0A6J1IFU4 (glutathione S-transferase DHAR2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476861 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.6e-98
Identity = 187/241 (77.59%), Postives = 197/241 (81.74%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MA+EV  KAA GAPDV+GDC                            PF  RVLLTLEE
Sbjct: 1   MAIEVVVKAATGAPDVLGDC----------------------------PFCHRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+ YKL L+NLSDKPSWFLEVSPEGKVPVVKFD KWVPDSDVIVGILEEKYP+PSLVTP
Sbjct: 61  KKVAYKLLLVNLSDKPSWFLEVSPEGKVPVVKFDGKWVPDSDVIVGILEEKYPDPSLVTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLS 180
           P+FSSVGSKINGAFVKFL+SKD  DGSEQALLEELKALD+HLKAHGPYVAGEKVTA DLS
Sbjct: 121 PQFSSVGSKINGAFVKFLLSKDPTDGSEQALLEELKALDEHLKAHGPYVAGEKVTAVDLS 180

Query: 181 LAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVNA 240
           LAPKLY ID+ALGHFKKW FPENL+SLSAYKKLLFARESFVKTKA PEHVIAGW  KVNA
Sbjct: 181 LAPKLYHIDIALGHFKKWVFPENLSSLSAYKKLLFARESFVKTKAVPEHVIAGWETKVNA 213

Query: 241 A 242
           A
Sbjct: 241 A 213

[Close]

BLAST of MS017319 vs. TAIR 10
Match: AT1G75270.1 (dehydroascorbate reductase 2 )

HSP 1 Score: 310.1 bits (793), Expect = 1.6e-84
Identity = 156/241 (64.73%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MAL++  K AVGAPDV+GDC                            PF+QRVLLTLEE
Sbjct: 1   MALDICVKVAVGAPDVLGDC----------------------------PFSQRVLLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           KK+PYK HLIN+SDKP WFL++SPEGKVPVVK D KWV DSDVIVG+LEEKYPEPSL TP
Sbjct: 61  KKLPYKTHLINVSDKPQWFLDISPEGKVPVVKLDGKWVADSDVIVGLLEEKYPEPSLKTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAH-GPYVAGEKVTAADL 180
           PEF+SVGSKI GAFV FL SKD+NDGSE+AL++EL+AL+ HLK H GP+VAGEK+TA DL
Sbjct: 121 PEFASVGSKIFGAFVTFLKSKDANDGSEKALVDELEALENHLKTHSGPFVAGEKITAVDL 180

Query: 181 SLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           SLAPKLY ++VALGH+K W+ PE+L S+  Y K LF+RESF  TKA  E V+AGW  KVN
Sbjct: 181 SLAPKLYHLEVALGHYKNWSVPESLTSVRNYAKALFSRESFENTKAKKEIVVAGWESKVN 213

[Close]

BLAST of MS017319 vs. TAIR 10
Match: AT1G19570.1 (dehydroascorbate reductase )

HSP 1 Score: 285.8 bits (730), Expect = 3.2e-77
Identity = 145/240 (60.42%), Postives = 172/240 (71.67%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MALE+  KAAVGAPD +GDC                            PF+QR LLTLEE
Sbjct: 1   MALEICVKAAVGAPDHLGDC----------------------------PFSQRALLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           K + YK+HLINLSDKP WFL++SP+GKVPV+K DDKWV DSDVIVGILEEKYP+P L TP
Sbjct: 61  KSLTYKIHLINLSDKPQWFLDISPQGKVPVLKIDDKWVTDSDVIVGILEEKYPDPPLKTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAH-GPYVAGEKVTAADL 180
            EF+SVGS I G F  FL SKDSNDGSE ALL EL+AL+ HLK+H GP++AGE+V+A DL
Sbjct: 121 AEFASVGSNIFGTFGTFLKSKDSNDGSEHALLVELEALENHLKSHDGPFIAGERVSAVDL 180

Query: 181 SLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           SLAPKLY + VALGHFK W+ PE+   +  Y K LF+ +SF KTK   ++VI+GWA KVN
Sbjct: 181 SLAPKLYHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKYVISGWAPKVN 212

[Close]

BLAST of MS017319 vs. TAIR 10
Match: AT1G19570.2 (dehydroascorbate reductase )

HSP 1 Score: 276.9 bits (707), Expect = 1.5e-74
Identity = 144/240 (60.00%), Postives = 171/240 (71.25%), Query Frame = 0


Query: 1   MALEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEE 60
           MALE+  KAAVGAPD +GDC                            PF+QR LLTLEE
Sbjct: 1   MALEICVKAAVGAPDHLGDC----------------------------PFSQRALLTLEE 60

Query: 61  KKIPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTP 120
           K + YK+HLINLSDKP  FL++SP+GKVPV+K DDKWV DSDVIVGILEEKYP+P L TP
Sbjct: 61  KSLTYKIHLINLSDKPQ-FLDISPQGKVPVLKIDDKWVTDSDVIVGILEEKYPDPPLKTP 120

Query: 121 PEFSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAH-GPYVAGEKVTAADL 180
            EF+SVGS I G F  FL SKDSNDGSE ALL EL+AL+ HLK+H GP++AGE+V+A DL
Sbjct: 121 AEFASVGSNIFGTFGTFLKSKDSNDGSEHALLVELEALENHLKSHDGPFIAGERVSAVDL 180

Query: 181 SLAPKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           SLAPKLY + VALGHFK W+ PE+   +  Y K LF+ +SF KTK   ++VI+GWA KVN
Sbjct: 181 SLAPKLYHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKYVISGWAPKVN 211

[Close]

BLAST of MS017319 vs. TAIR 10
Match: AT5G16710.1 (dehydroascorbate reductase 1 )

HSP 1 Score: 256.5 bits (654), Expect = 2.1e-68
Identity = 124/236 (52.54%), Postives = 163/236 (69.07%), Query Frame = 0


Query: 3   LEVAAKAAVGAPDVIGDCMFFFPTGNLFEFLLILTLGFFFSLSPAGPFTQRVLLTLEEKK 62
           LE+  KA++  P+ +GDC                            PF Q+VLLT+EEK 
Sbjct: 49  LEICVKASITTPNKLGDC----------------------------PFCQKVLLTMEEKN 108

Query: 63  IPYKLHLINLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPE 122
           +PY + +++LS+KP WFL++SPEGKVPVVKFD+KWVPDSDVI   LEEKYPEP L TPPE
Sbjct: 109 VPYDMKMVDLSNKPEWFLKISPEGKVPVVKFDEKWVPDSDVITQALEEKYPEPPLATPPE 168

Query: 123 FSSVGSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAHGPYVAGEKVTAADLSLA 182
            +SVGSKI   FV FL SKDS DG+EQ LL+EL   + ++K +GP++ GEK++AADLSLA
Sbjct: 169 KASVGSKIFSTFVGFLKSKDSGDGTEQVLLDELTTFNDYIKDNGPFINGEKISAADLSLA 228

Query: 183 PKLYQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKV 239
           PKLY + +ALGH+K W+ P++L  + +Y + +F+RESF  T+A  E VIAGW  KV
Sbjct: 229 PKLYHMKIALGHYKNWSVPDSLPFVKSYMENVFSRESFTNTRAETEDVIAGWRPKV 256

[Close]

BLAST of MS017319 vs. TAIR 10
Match: AT1G19550.1 (Glutathione S-transferase family protein )

HSP 1 Score: 169.1 bits (427), Expect = 4.4e-42
Identity = 88/174 (50.57%), Postives = 112/174 (64.37%), Query Frame = 0


Query: 69  LINLSDKPS--WFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSV 128
           ++ +   PS   F ++SP+GKVPV+K DDKWV DSD  VGILEEKYP+P L TP EF+SV
Sbjct: 3   VVQICSSPSHEMFWDISPQGKVPVLKIDDKWVTDSDATVGILEEKYPDPPLKTPAEFASV 62

Query: 129 GSKINGAFVKFLMSKDSNDGSEQALLEELKALDKHLKAH-GPYVAGEKVTAADLSLAPKL 188
           GS I                         +AL+ HLK+H GP++AGE+V+A DLSLAPKL
Sbjct: 63  GSNI------------------------FEALENHLKSHDGPFIAGERVSAVDLSLAPKL 122

Query: 189 YQIDVALGHFKKWTFPENLASLSAYKKLLFARESFVKTKAAPEHVIAGWALKVN 240
           Y + VALGHFK W+ PE+   +  Y K LF+ +SF KTK   + VI+GWA KVN
Sbjct: 123 YHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKCVISGWAPKVN 152

The following BLAST results are available for this feature:

BLAST of MS017319 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139338.1	1.0e-109	87.55	glutathione S-transferase DHAR2-like [Momordica charantia]	[more]
XP_022985936.1	3.1e-98	78.01	uncharacterized protein LOC111483829 isoform X1 [Cucurbita maxima]	[more]
XP_022985944.1	3.1e-98	78.01	probable glutathione S-transferase DHAR2, chloroplastic isoform X2 [Cucurbita ma...	[more]
XP_022985954.1	3.1e-98	78.01	uncharacterized protein LOC111483829 isoform X3 [Cucurbita maxima]	[more]
XP_022976477.1	5.3e-98	77.59	glutathione S-transferase DHAR2-like isoform X2 [Cucurbita maxima]	[more]

BLAST of MS017319 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FRL8	2.2e-83	64.73	Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV...	[more]
Q9FWR4	4.5e-76	60.42	Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 G...	[more]
Q65XA0	5.9e-76	58.92	Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japon...	[more]
Q9FG59	2.3e-72	59.02	Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR...	[more]
Q67UK9	1.0e-67	53.39	Probable glutathione S-transferase DHAR2, chloroplastic OS=Oryza sativa subsp. j...	[more]

BLAST of MS017319 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CFE5	5.0e-110	87.55	glutathione S-transferase DHAR2-like OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1JER3	1.5e-98	78.01	uncharacterized protein LOC111483829 isoform X3 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1JEQ3	1.5e-98	78.01	probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita ...	[more]
A0A6J1J9M2	1.5e-98	78.01	uncharacterized protein LOC111483829 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IFU4	2.6e-98	77.59	glutathione S-transferase DHAR2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]

BLAST of MS017319 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75270.1	1.6e-84	64.73	dehydroascorbate reductase 2	[more]
AT1G19570.1	3.2e-77	60.42	dehydroascorbate reductase	[more]
AT1G19570.2	1.5e-74	60.00	dehydroascorbate reductase	[more]
AT5G16710.1	2.1e-68	52.54	dehydroascorbate reductase 1	[more]
AT1G19550.1	4.4e-42	50.57	Glutathione S-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.1050.10		coord: 118..240 e-value: 5.4E-45 score: 154.3
None	No IPR available	PFAM	PF13410	GST_C_2	coord: 145..206 e-value: 3.0E-8 score: 33.6
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 43..117 e-value: 2.0E-25 score: 90.7
None	No IPR available	SFLD	SFLDG00358	Main_(cytGST)	coord: 47..202 e-value: 3.1E-27 score: 90.3
None	No IPR available	CDD	cd00570	GST_N_family	coord: 45..105 e-value: 4.8065E-15 score: 66.056
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF13417	GST_N_3	coord: 46..115 e-value: 5.0E-17 score: 62.0
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 38..116 score: 15.876472
IPR040079	Glutathione Transferase family	SFLD	SFLDS00019	Glutathione_Transferase_(cyto	coord: 47..202 e-value: 3.1E-27 score: 90.3
IPR044627	Dehydroascorbate reductases DHAR1/2/3/4	PANTHER	PTHR44420	GLUTATHIONE S-TRANSFERASE DHAR2-RELATED	coord: 2..236
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 112..234 score: 10.467372
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 45..115
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 127..234

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017319.1	MS017319.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033355	ascorbate glutathione cycle
biological_process	GO:0098869	cellular oxidant detoxification
biological_process	GO:0006749	glutathione metabolic process
molecular_function	GO:0045174	glutathione dehydrogenase (ascorbate) activity
molecular_function	GO:0005515	protein binding