MS017289 (gene) Bitter gourd (TR) v1

Overview
NameMS017289
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionElp3 domain-containing protein
Locationscaffold33: 1365488 .. 1369664 (+)
RNA-Seq ExpressionMS017289
SyntenyMS017289
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGCCATTTCAATGGCGCTCATTCACCATGTATGCTCCACATCCGCCGCACGCGCAGTACGGCCTCGCCGCGTCGCCGCCGTGCCCTCTCGGAACCTCTCCACGTCCATATCCCCTCGCGTACGCCCCCCTCCCGAAGCTTCGAAAGTGCTCATCGGCTTGTCGGAGCAGGAGCTCCAACAGCTCGCTTTGGACTTCGGCCAGGTAACAAACGATAAACGACTGTATTAGTCTAAAAGCCTGTTTTGGATTGCTCGCAAATGAGTGGCTTCATTTTGTGGAGCTTTTCGCTTCTGTTTCGTTAAATTGTTGGAGATGTTTCTGTTTGGGATGAATTAGGAAAAGTATAGAGGTAAGCAACTCCACCAGCTCATTTACAAAAGGAAGCTCAAGGAAATTGAAGAGTTTACCAACTGTCAGTATTATTTCTTCTTCTCTGATTAACTTGGTCTGTCTTTACTTTATCTTGTTATTGAGTTGCTGTCTGTTATGGTGATTTGGGTTGATTGCAGTGCCACAGGCATTCAGGAATGGTCTTCAGGAAGCTGGATGGAGGGTGGGCCGTTCTCCTGTTTACCAGTCTGTTACTGCATCTGACGGGACCGTTAAGGTACTTTGATATTAAGATCATTCATTGCTTTATCCGATTTTGATTTAATTGAATTGCAGTTGTTCACTGTTTCAATTGTTTTCTGTTTGCATTCTTATTAAATTATTCTAAATTTATCATGGTAAGTGAATGGTTGAATGTTTGTACACGAGTCTGTTTGGCTTTAATGCATCCATACGTCTAAATTTTGCAGTTGTTGCTGAAGTTGGATGACAACAGGCTGATTGAAACCGTTGGCATACCTGTGGAAGACAAAGACAATAAAGGTTCAGTTCGCCTTACTGCTTGCATCTCATCACAGGTAATATTCATATTTGATCATTCAGAGACGTGAATTCTGTCATTTACATTTTCATCAATGTATCTTAGTTTATGATTTGGTCACAGAGTATTTCTCTTCTTAGTAAAGTGATTGGGCGTTAATGTAGTACCCGGTAGTAGTCTAGCCCCATGTATACTTGAGAAATAACCACATTGCCCAATAGATGAAATTACGGTACATATATGTCTTTTGAAAGCACTTGCAATGATTTTTTAACCGGTTCCCTGGGAAATGGGATTGTCTGAACTCATTATGGTGTTCAATCTTAAATCAAATTTATACTGAATTTGAAACATTAAGGTTGGCTGCCCATTGCGCTGTTCATTTTGTGCCACTGGAAAAGGGGGGTTTTCAAGGAATCTTCAGAGACATGAAATTGTTGAACAGGTATTTTATATCTCTTACATTTTGGTTTCGTGTTTTTGTTATGGATTTTTTCTGTTCTAGTATCTCATTGATTTTTAGAAATTTGAATAAATGAAATTATTTCAGGTGTAATTAATCCTCCCCATTGTGATTAGTTGCATTTTATATACACAGGTATTTGCGATTGAGGATATTTTCAACCATAGGGTGACAAATGTAGTATTCATGGGAATGGGCGAGCCAATGTTAAATATGAAATCGGTGCTTGAAGCGCATCAGTGCTTGAACAAGGTTGATTCTTCAAGACTCATTTATCCTAACATTTTGTGGGGTAATTTTTTGTGTACTATTTGATCTTTTCCCCTTTGTTATTCTTCCGTTCTATATTTTCTTTTTACCCGGTTCCGGTACATACACTGCAAGAGAGGACCTGGAGAGCAAAACTCTTCATTTACTAGCAAAACAAGTGCATTGAAAGAATGAAATACACAAGGAAAGGCCCCCCAGAGAATACAGCATTACAAGAAAACTCTCAAATTGGTAAAATTAAAATATAATTATGAGAAACCTTATGAGAGAGCTCTGAGAGGAGGAAAAAACAACAGTTGCCGAGATCTGGCCCAAAAATATATCCATCAAGCACCACATAAAAAATTCTTCTATTTCTTCTAACCACCAAATATCTCATAAAAAGTTCTAATAGCAATAAGCCATAGGGTATTAAATTTTCTTTTTACAAGGTAATTGCGTTTCACACATCTAAGAGCAACCAAGCGAAAGAGCAAGATTACACTATAGAAGTGTGAACTTATTGTAGAATGATTCTTAGATTCAATTGAATTCCAAGATATAGGTATTCTATTTGAGTCATCCCATTTTATGCAAAGAGAACCCATCCAGGAGATAAGGAGACATTGCTTGGCTTTTTTCCTGAGTACTAAACCGCCCCAGAGGGTAGACCAAATGAAAACCTTTCTTTTTTCCTTTTTTGGGTATCCATCCTTATGCCTTCCCAAGCTTCTGGTTTGGTGCATTTAAGCACAAAACTGTCTTTAGATCATCCCCAAACAACCCCATATATTTTTTGAGGTTCTATCAGTATCATGCCTGTCCACTTTGAGATAAAGGTTCTTATGTCATTATTATCTTTTAATAATAAAATGTTATCACATGAAACTGTCATGGGAAGGCTTGCTTTAAAGTTCCACCATGTTCATTACACACTAATTTTATGTGCCGAGTCTGCAATTTCTCATGAAATTTTTTATGTTTTTTCTAGGACGTTCAAATTGGACAAAGAATGATCACGATTTCCACTGTGGGTGTTCCTAACACAATTAAAAGGTTGGCGTCTCACAAGCTTCAGTCAACATTGGCTGTCAGGTAATATATACTCAACAGGAAAAATATTATAGATTGTTTGATTAAATCAAAAACAGAAAACAAAAAATTCAGTCATTTTTAGTGATATTTTGCCAAGGGATAAATTTTGCTAGTTTTCATGTACTGCTTTGTGATATATAATTATACGTCCATTTTAAGAGTGTAGGTCTTTTCTTTCCAGCCAACTTATGCTGTCACCCCTAAATTTTTAATAGGTAGCAGTTAATTAAGAACTGTGCTAGTTGGAGAATTTTGGTGTAGTTTATTGTTACGACTCAAAATTTTGCCATTCCAAATATCTGAGTGAAGATGTCTATGTTTACGGTGGTTGATTGCTTTCTTATGGTTGTCCCAATAAATTCTGTTTACTTATTTAGCAGTTAGATTACTGATTTCTCACTGATTATAACTATTTTTTAAAAATGTTTTTAATTAATGGAGAAGACATGCAAAAGAGGGAGAGGTGAGAAATCCTCCCAAATGCATAGGATGTTACAGAACGGTGTTCCAACTTCCAATTATTGTAAATCATAATCAGGGAATAACTACAAAAACGTTAAGAGATTGTACTAGCCTACTAGGCAGACCCCTTCCCATTGTTCTTTATGAGTTTTTTCTGGCCTCAATTTTTTTTTTCTCTCCCTAATCTTTCAAACCATATGGTCCATTCCAAAGCAAAATTTAATACTTTTAATCCTTTATTTTGAAGAGATGCCCATTACCATTTGTAGGATGAGGTCCTTAGATTTTGGCTCTCTTTTTAAATTCTACTTCTATGAACTCTTCATTTTCTGTCCATCAGCTTACATGCACCAAACCAGAAGCTTCGGGAGACTATTGTGCCCAGTGCAAAAGCATATCCTCTTGAAGCGATTATGAAGGATTGTCGCGATTATTTCCTCGAAACTAGTCGGAGAGTTTCCTTTGAGTATGCACTTTTAGGTACTGGATTCACTTTAGCACCACTATCCCTCTCTCTTCTTCCATTGTGTTCCTCGTTGAAGATTTGGGCTTCCAAAACATGACATTCTTCCCCCCTGATTTTTCTGGTGCTTATCAATCGAACAGCCGGAGTCAATGATGCAGTAGAGCATGCCGTAGAACTTGCAAAGCTTCTCCACGAATGGGGGCGCGGTTATCACGTGAACCTGATACCTTTCAATCCCATAGAAGGCTCTGAGTATCAGCGCCCATATAAGAAAGCAGTAAGATGCTCTTAACAGATGCGCTGTTTCCCATCACGTATCAAGAAGAAAATGTTTCAGATTTTGTATGTATACTTTTCACTGATCAATGATCTTTTCGTTTATCTTCAGGTGGTGGCATTTGTAGCTGCTCTAGAGTCCCGTAAAATAACCGTAAGCGTTCGTCAAACAAGAGGCCTGGATGCAAATGCAGCTTGTGGTCAGCTAAGAAACCAATTCCAGAAGGACCCTTTGGTCGCCGATTCTGACAGCAATCGGTCCGACGAATCAGCTGTAGCTATGGCTTGT

mRNA sequence

ATGATTGCCATTTCAATGGCGCTCATTCACCATGTATGCTCCACATCCGCCGCACGCGCAGTACGGCCTCGCCGCGTCGCCGCCGTGCCCTCTCGGAACCTCTCCACGTCCATATCCCCTCGCGTACGCCCCCCTCCCGAAGCTTCGAAAGTGCTCATCGGCTTGTCGGAGCAGGAGCTCCAACAGCTCGCTTTGGACTTCGGCCAGGAAAAGTATAGAGGTAAGCAACTCCACCAGCTCATTTACAAAAGGAAGCTCAAGGAAATTGAAGAGTTTACCAACTTGCCACAGGCATTCAGGAATGGTCTTCAGGAAGCTGGATGGAGGGTGGGCCGTTCTCCTGTTTACCAGTCTGTTACTGCATCTGACGGGACCGTTAAGTTGTTGCTGAAGTTGGATGACAACAGGCTGATTGAAACCGTTGGCATACCTGTGGAAGACAAAGACAATAAAGGTTCAGTTCGCCTTACTGCTTGCATCTCATCACAGGTTGGCTGCCCATTGCGCTGTTCATTTTGTGCCACTGGAAAAGGGGGGTTTTCAAGGAATCTTCAGAGACATGAAATTGTTGAACAGGTATTTGCGATTGAGGATATTTTCAACCATAGGGTGACAAATGTAGTATTCATGGGAATGGGCGAGCCAATGTTAAATATGAAATCGGTGCTTGAAGCGCATCAGTGCTTGAACAAGGACGTTCAAATTGGACAAAGAATGATCACGATTTCCACTGTGGGTGTTCCTAACACAATTAAAAGGTTGGCGTCTCACAAGCTTCAGTCAACATTGGCTGTCAGCTTACATGCACCAAACCAGAAGCTTCGGGAGACTATTGTGCCCAGTGCAAAAGCATATCCTCTTGAAGCGATTATGAAGGATTGTCGCGATTATTTCCTCGAAACTAGTCGGAGAGTTTCCTTTGAGTATGCACTTTTAGCCGGAGTCAATGATGCAGTAGAGCATGCCGTAGAACTTGCAAAGCTTCTCCACGAATGGGGGCGCGGTTATCACGTGAACCTGATACCTTTCAATCCCATAGAAGGCTCTGAGTATCAGCGCCCATATAAGAAAGCAGTGGTGGCATTTGTAGCTGCTCTAGAGTCCCGTAAAATAACCGTAAGCGTTCGTCAAACAAGAGGCCTGGATGCAAATGCAGCTTGTGGTCAGCTAAGAAACCAATTCCAGAAGGACCCTTTGGTCGCCGATTCTGACAGCAATCGGTCCGACGAATCAGCTGTAGCTATGGCTTGT

Coding sequence (CDS)

ATGATTGCCATTTCAATGGCGCTCATTCACCATGTATGCTCCACATCCGCCGCACGCGCAGTACGGCCTCGCCGCGTCGCCGCCGTGCCCTCTCGGAACCTCTCCACGTCCATATCCCCTCGCGTACGCCCCCCTCCCGAAGCTTCGAAAGTGCTCATCGGCTTGTCGGAGCAGGAGCTCCAACAGCTCGCTTTGGACTTCGGCCAGGAAAAGTATAGAGGTAAGCAACTCCACCAGCTCATTTACAAAAGGAAGCTCAAGGAAATTGAAGAGTTTACCAACTTGCCACAGGCATTCAGGAATGGTCTTCAGGAAGCTGGATGGAGGGTGGGCCGTTCTCCTGTTTACCAGTCTGTTACTGCATCTGACGGGACCGTTAAGTTGTTGCTGAAGTTGGATGACAACAGGCTGATTGAAACCGTTGGCATACCTGTGGAAGACAAAGACAATAAAGGTTCAGTTCGCCTTACTGCTTGCATCTCATCACAGGTTGGCTGCCCATTGCGCTGTTCATTTTGTGCCACTGGAAAAGGGGGGTTTTCAAGGAATCTTCAGAGACATGAAATTGTTGAACAGGTATTTGCGATTGAGGATATTTTCAACCATAGGGTGACAAATGTAGTATTCATGGGAATGGGCGAGCCAATGTTAAATATGAAATCGGTGCTTGAAGCGCATCAGTGCTTGAACAAGGACGTTCAAATTGGACAAAGAATGATCACGATTTCCACTGTGGGTGTTCCTAACACAATTAAAAGGTTGGCGTCTCACAAGCTTCAGTCAACATTGGCTGTCAGCTTACATGCACCAAACCAGAAGCTTCGGGAGACTATTGTGCCCAGTGCAAAAGCATATCCTCTTGAAGCGATTATGAAGGATTGTCGCGATTATTTCCTCGAAACTAGTCGGAGAGTTTCCTTTGAGTATGCACTTTTAGCCGGAGTCAATGATGCAGTAGAGCATGCCGTAGAACTTGCAAAGCTTCTCCACGAATGGGGGCGCGGTTATCACGTGAACCTGATACCTTTCAATCCCATAGAAGGCTCTGAGTATCAGCGCCCATATAAGAAAGCAGTGGTGGCATTTGTAGCTGCTCTAGAGTCCCGTAAAATAACCGTAAGCGTTCGTCAAACAAGAGGCCTGGATGCAAATGCAGCTTGTGGTCAGCTAAGAAACCAATTCCAGAAGGACCCTTTGGTCGCCGATTCTGACAGCAATCGGTCCGACGAATCAGCTGTAGCTATGGCTTGT

Protein sequence

MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC
Homology
BLAST of MS017289 vs. NCBI nr
Match: XP_022139334.1 (uncharacterized protein LOC111010274 [Momordica charantia])

HSP 1 Score: 808.5 bits (2087), Expect = 2.6e-230
Identity = 413/417 (99.04%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIAISMALIHHVCS SAARAVRPRRVAAVPSRNLSTS SPR RPPPEASKVLIGLSEQEL
Sbjct: 1   MIAISMALIHHVCSXSAARAVRPRRVAAVPSRNLSTSTSPRARPPPEASKVLIGLSEQEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLALDFGQEKYRGKQLHQLIYKRK+KEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKQLHQLIYKRKVKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC
Sbjct: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 417

BLAST of MS017289 vs. NCBI nr
Match: XP_038892136.1 (probable dual-specificity RNA methyltransferase RlmN [Benincasa hispida])

HSP 1 Score: 749.2 bits (1933), Expect = 1.9e-212
Identity = 384/417 (92.09%), Postives = 400/417 (95.92%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MI ISMALIH VCS S ARA+RPR VAAVPSRNLSTSISP  R  PEA KVLIGLS+QEL
Sbjct: 2   MIPISMALIHQVCSPSVARALRPRCVAAVPSRNLSTSISPPARASPEAPKVLIGLSDQEL 61

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLA+DFGQEKYRGKQLHQLIYKRK+KEIEEFTNLP AFRN LQEAGW+VGRSPVYQSVT
Sbjct: 62  QQLAVDFGQEKYRGKQLHQLIYKRKVKEIEEFTNLPLAFRNDLQEAGWKVGRSPVYQSVT 121

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK+NKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 122 ASDGTVKLLLKLDDNRLIETVGIPVEDKNNKGSVRLTACISSQVGCPLRCSFCATGKGGF 181

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 182 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 241

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+MKDCRDYFL+
Sbjct: 242 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEALMKDCRDYFLQ 301

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVEL+KLLHEWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 302 TSRRVSFEYALLAGVNDAVEHAVELSKLLHEWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 361

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+ DSD ++S ESAVA+AC
Sbjct: 362 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLVDSDISQS-ESAVAVAC 417

BLAST of MS017289 vs. NCBI nr
Match: XP_022957587.1 (uncharacterized protein LOC111458934 [Cucurbita moschata])

HSP 1 Score: 743.0 bits (1917), Expect = 1.4e-210
Identity = 380/417 (91.13%), Postives = 400/417 (95.92%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIA+SMALIH VCS S ARA+RPR VAAVPSR  STSISP  R  PE+ KVLIGLSE+EL
Sbjct: 1   MIAVSMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLA+D GQEKYRGKQLHQLIYKRK+KE+EEFTNLP AFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF++
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+AD+DS++S ESAVA+AC
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQS-ESAVAVAC 416

BLAST of MS017289 vs. NCBI nr
Match: XP_022989725.1 (uncharacterized protein LOC111486721 [Cucurbita maxima])

HSP 1 Score: 743.0 bits (1917), Expect = 1.4e-210
Identity = 381/417 (91.37%), Postives = 399/417 (95.68%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MI ISMALIH VCS S ARA+RPR VAAVPSR  STSISP  R  PE+ KVLIGLSE+EL
Sbjct: 1   MITISMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLA+D GQEKYRGKQLHQLIYKRK+KE+EEFTNLP AFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFL+
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+AD+DS++S ESAVA+AC
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQS-ESAVAVAC 416

BLAST of MS017289 vs. NCBI nr
Match: KAG7031617.1 (rlmN, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 741.1 bits (1912), Expect = 5.2e-210
Identity = 380/417 (91.13%), Postives = 399/417 (95.68%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIAISMALIH VCS S ARA+RPR VAAVPSR  ST ISP  R  PE+ KVLIGLSE+EL
Sbjct: 1   MIAISMALIHQVCSPSVARALRPRCVAAVPSRKSSTFISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLA+D GQEKYRGKQLHQLIYKRK+KE+EEFTNLP AFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF++
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+AD+DS++S ESAVA+AC
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQS-ESAVAVAC 416

BLAST of MS017289 vs. ExPASy Swiss-Prot
Match: Q2JRQ8 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=rlmN PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.7e-100
Identity = 191/346 (55.20%), Postives = 247/346 (71.39%), Query Frame = 0

Query: 52  LIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVG 111
           L+G S   L+  A++ GQ  YRG+QLH  +Y + ++ ++E T  P+A+R  LQE  + VG
Sbjct: 8   LLGQSLAALKDWAVEQGQPAYRGQQLHTWLYHKGIRSLQEVTVFPKAWREALQE--YPVG 67

Query: 112 RSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCS 171
           RS V Q + + DGTVK LL+L D  LIETVGIP        + RLT C+SSQVGCP+ C+
Sbjct: 68  RSQVVQRIESRDGTVKFLLQLADGELIETVGIPT-------ARRLTVCVSSQVGCPMACN 127

Query: 172 FCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNK 231
           FCATGK G+ RNL+ HEI++QV  +++ F  RV++VVFMGMGEP+LN  +V++A + LN+
Sbjct: 128 FCATGKMGYRRNLKLHEILDQVLTVQEDFGRRVSHVVFMGMGEPLLNRDTVVQAIRSLNQ 187

Query: 232 DVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIM 291
           D+ IGQR IT+STVGVP  I  LA   LQ TLAVSLHAPNQ+LR+ ++PSA  YPL+A++
Sbjct: 188 DIGIGQRHITLSTVGVPRQIPWLAQQDLQITLAVSLHAPNQELRQQLIPSAAHYPLDALI 247

Query: 292 KDCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLH---EWGRGYHVNLIPFNPIEG 351
           +DCRDY L + RR+SFEY LLAGVND   HA +LA LL    + G   HVNLIP+NPI  
Sbjct: 248 QDCRDYMLCSGRRISFEYTLLAGVNDLPIHARQLAHLLRQASQAGARLHVNLIPYNPIAE 307

Query: 352 SEYQRPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQF 395
           ++YQRP+   V  FV  LE   + VSVRQTRGLD+NAACGQLR  F
Sbjct: 308 ADYQRPHPTRVAEFVRLLEEHHVQVSVRQTRGLDSNAACGQLRGSF 344

BLAST of MS017289 vs. ExPASy Swiss-Prot
Match: Q2JMN2 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=rlmN PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 8.6e-99
Identity = 188/346 (54.34%), Postives = 242/346 (69.94%), Query Frame = 0

Query: 52  LIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVG 111
           L+G S   L+  A+  GQ  YRG+QLH  IY++ ++ +E+ T  P+A+R  +Q   + VG
Sbjct: 8   LLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQ--SYPVG 67

Query: 112 RSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCS 171
           RS + Q   A DGTVK LL L D +LIETVGIP        + RLT C+SSQVGCP+ C 
Sbjct: 68  RSRIVQRTEARDGTVKFLLGLADGQLIETVGIPT-------AKRLTVCVSSQVGCPMACD 127

Query: 172 FCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNK 231
           FCATGK G+ RNL+ HEI++QV  +++ F  RV++VVFMGMGEP+LN  +V++A + LN+
Sbjct: 128 FCATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMGEPLLNRDTVVQAIRSLNQ 187

Query: 232 DVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIM 291
           D+ IGQR IT+STVGVP  I  LA   LQ TLAVSLHAPNQ LR+ ++PSA  YPL+ ++
Sbjct: 188 DIGIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTLI 247

Query: 292 KDCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRG---YHVNLIPFNPIEG 351
           +DCRDY L T RRVSFEY LL+GVND   HA +LA+LL +  R     HVNLIP+NPI  
Sbjct: 248 QDCRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPISE 307

Query: 352 SEYQRPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQF 395
           ++YQRP+   V  FV  LE  ++  +VRQTRGLD NAACGQLR  F
Sbjct: 308 ADYQRPHPTRVREFVRQLEQHQVRATVRQTRGLDGNAACGQLRGSF 344

BLAST of MS017289 vs. ExPASy Swiss-Prot
Match: B0C9F4 (Probable dual-specificity RNA methyltransferase RlmN OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=rlmN PE=3 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 5.6e-98
Identity = 187/352 (53.12%), Postives = 246/352 (69.89%), Query Frame = 0

Query: 47  EASKVLIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEA 106
           E+   L+G S+ +L   A  + Q  YRG+Q+HQ IY++ +  + + T LP+ +R  +  A
Sbjct: 8   ESIPPLLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQWRTEI--A 67

Query: 107 GWRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGC 166
              VGRS ++    A DGTVK LLKL D ++IETVGIP +        RLT C+SSQVGC
Sbjct: 68  DIPVGRSQIHHRSAAQDGTVKYLLKLADGQIIETVGIPTQK-------RLTVCVSSQVGC 127

Query: 167 PLRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAH 226
           P+ C FCATGKG + RNL  HEIV+QV  +++ F  RV NVVFMGMGEP+LN++ VL A 
Sbjct: 128 PMGCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVVFMGMGEPLLNLEQVLAAV 187

Query: 227 QCLNKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYP 286
           + LN+DV IGQR +T+STVG+P  I +LA H+LQ TLAVSLHA NQ++R  +VPSAK YP
Sbjct: 188 RSLNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYP 247

Query: 287 LEAIMKDCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPI 346
           LE ++KDCR Y  +T RRV+FEY +L+GVND  EHA+ELA   H  G   HVNLIP+NPI
Sbjct: 248 LEKLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAH--HLRGFQSHVNLIPYNPI 307

Query: 347 EGSEYQRPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDP 399
              +YQRP +K +  F+ +L+S+ IT S+R++RGLD +AACGQLR     DP
Sbjct: 308 SEVDYQRPTQKQLQQFLNSLQSQHITASIRRSRGLDKDAACGQLRATQMIDP 348

BLAST of MS017289 vs. ExPASy Swiss-Prot
Match: Q8DG98 (Probable dual-specificity RNA methyltransferase RlmN OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=rlmN PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.6e-97
Identity = 185/341 (54.25%), Postives = 239/341 (70.09%), Query Frame = 0

Query: 51  VLIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRV 110
           VL+G S  EL+      GQ  YRG+QLHQ +Y++  + ++E T  P+ +R  L +    +
Sbjct: 2   VLLGRSAAELKAWVEAQGQPGYRGQQLHQWLYRKGARSLQEITVFPKQWRAALADV--EI 61

Query: 111 GRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRC 170
           GRS +     A DGTVKLLL L D   IETVGIP  D       RLT C+SSQVGCP+ C
Sbjct: 62  GRSEIRYRHDAQDGTVKLLLALADGETIETVGIPSSD-------RLTVCVSSQVGCPMAC 121

Query: 171 SFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLN 230
            FCATGKGG+ RNL  HEI++QV  I+     RV++VVFMGMGEP+LN+ +VL+A  CLN
Sbjct: 122 DFCATGKGGYRRNLACHEILDQVLTIQSEMGRRVSHVVFMGMGEPLLNLPAVLQAITCLN 181

Query: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 290
           +D+ IGQR ITISTVG+P  I+RLA H+LQ+TLAVSLHAPNQ LRE ++PSAK YPL  +
Sbjct: 182 RDIGIGQRHITISTVGIPQQIQRLAQHQLQTTLAVSLHAPNQALREQLIPSAKHYPLSQL 241

Query: 291 MKDCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSE 350
           + DCR Y  +T RR++FEY +LAGVND  +HA ELA+LL   G   HVNLIP+NPI  + 
Sbjct: 242 IADCRAYVQQTGRRITFEYTVLAGVNDRPQHAEELAQLLR--GFQSHVNLIPYNPIAEAA 301

Query: 351 YQRPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLR 392
           YQRP  + +  F++ L++  +T S+R++RGLD  AACGQLR
Sbjct: 302 YQRPTDQHLRQFLSQLQALGVTASIRRSRGLDRQAACGQLR 331

BLAST of MS017289 vs. ExPASy Swiss-Prot
Match: Q7U8K0 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain WH8102) OX=84588 GN=rlmN PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 4.0e-96
Identity = 184/351 (52.42%), Postives = 238/351 (67.81%), Query Frame = 0

Query: 51  VLIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRV 110
           +L+G S  EL+  A+  GQ+ +RG+QLH  +Y +  + + E T LP+A+R  L+E G  V
Sbjct: 1   MLLGRSAAELESWAVAQGQKPFRGRQLHDWLYAKGARSLSEITVLPKAWRESLKEDGVEV 60

Query: 111 GRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRC 170
           GR        A+D T KLLL  DD   IETVGIP +        RLT C+SSQVGCP+ C
Sbjct: 61  GRLKEVHRSVAADATTKLLLSTDDGETIETVGIPTDQ-------RLTVCVSSQVGCPMAC 120

Query: 171 SFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLN 230
            FCATGKGG  R+L+ HEIV+QV ++ ++ + R +++VFMGMGEP+LN ++VL+A +CLN
Sbjct: 121 RFCATGKGGLQRSLRTHEIVDQVLSVREVMDRRPSHIVFMGMGEPLLNSQAVLDAIRCLN 180

Query: 231 KDVQIGQRMITISTVGVPNTIKRLASHKL------QSTLAVSLHAPNQKLRETIVPSAKA 290
            D+ IGQR IT+STVGVP T+ +LA   L      Q TLAVSLHAPNQ LRE ++P+A A
Sbjct: 181 DDLGIGQRRITVSTVGVPKTLPQLAELALATLGRAQFTLAVSLHAPNQALREELIPTAHA 240

Query: 291 YPLEAIMKDCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFN 350
           YP EA+++DCR Y   T RRVSFEY LL G+NDA EHA ELA  +   G   HVNLI +N
Sbjct: 241 YPYEALLEDCRHYLAVTGRRVSFEYILLGGLNDAPEHAAELADRV--GGFQSHVNLIAYN 300

Query: 351 PIEGSEYQRPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQFQ 396
           PIE  E+QRP +  +  F   LE R + VS+R +RGLD NAACGQLR + Q
Sbjct: 301 PIEEEEFQRPTRARIEGFQRVLERRGVAVSLRASRGLDQNAACGQLRRRRQ 342

BLAST of MS017289 vs. ExPASy TrEMBL
Match: A0A6J1CCN0 (uncharacterized protein LOC111010274 OS=Momordica charantia OX=3673 GN=LOC111010274 PE=3 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.3e-230
Identity = 413/417 (99.04%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIAISMALIHHVCS SAARAVRPRRVAAVPSRNLSTS SPR RPPPEASKVLIGLSEQEL
Sbjct: 1   MIAISMALIHHVCSXSAARAVRPRRVAAVPSRNLSTSTSPRARPPPEASKVLIGLSEQEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLALDFGQEKYRGKQLHQLIYKRK+KEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKQLHQLIYKRKVKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC
Sbjct: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 417

BLAST of MS017289 vs. ExPASy TrEMBL
Match: A0A6J1JN65 (uncharacterized protein LOC111486721 OS=Cucurbita maxima OX=3661 GN=LOC111486721 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.6e-211
Identity = 381/417 (91.37%), Postives = 399/417 (95.68%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MI ISMALIH VCS S ARA+RPR VAAVPSR  STSISP  R  PE+ KVLIGLSE+EL
Sbjct: 1   MITISMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLA+D GQEKYRGKQLHQLIYKRK+KE+EEFTNLP AFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFL+
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+AD+DS++S ESAVA+AC
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQS-ESAVAVAC 416

BLAST of MS017289 vs. ExPASy TrEMBL
Match: A0A6J1GZJ0 (uncharacterized protein LOC111458934 OS=Cucurbita moschata OX=3662 GN=LOC111458934 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.6e-211
Identity = 380/417 (91.13%), Postives = 400/417 (95.92%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIA+SMALIH VCS S ARA+RPR VAAVPSR  STSISP  R  PE+ KVLIGLSE+EL
Sbjct: 1   MIAVSMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLA+D GQEKYRGKQLHQLIYKRK+KE+EEFTNLP AFRNGLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF++
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+AD+DS++S ESAVA+AC
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQS-ESAVAVAC 416

BLAST of MS017289 vs. ExPASy TrEMBL
Match: A0A0A0KLM9 (Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G181470 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.3e-210
Identity = 380/425 (89.41%), Postives = 398/425 (93.65%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIAIS ALIH VCS S ARA+RPR VAAVPSRNLSTSISP  RP PEA KVLIGLS+QEL
Sbjct: 1   MIAISTALIHQVCSPSVARALRPRCVAAVPSRNLSTSISPSARPSPEAPKVLIGLSDQEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLALDFGQEKYRGKQLHQLIYKRK+KE+EEFTNLP  FR  LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLKFRTDLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           A+DGTVKLLLKLDDNRLIETVGIPVEDK+NKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 AADGTVKLLLKLDDNRLIETVGIPVEDKNNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTI+RLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+MKDCRDYFL+
Sbjct: 241 TISTVGVPNTIQRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEALMKDCRDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSD--------ESA 418
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+ DSD ++ +        E+A
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLVDSDISQPEAAEAAEAAEAA 420

BLAST of MS017289 vs. ExPASy TrEMBL
Match: A0A1S3CGI7 (probable dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103500206 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 7.3e-210
Identity = 380/417 (91.13%), Postives = 395/417 (94.72%), Query Frame = 0

Query: 1   MIAISMALIHHVCSTSAARAVRPRRVAAVPSRNLSTSISPRVRPPPEASKVLIGLSEQEL 60
           MIAIS ALIH VCS S ARA+R R VAAVPSRNLSTSISP   P PEA KVLIGLS+QEL
Sbjct: 1   MIAISTALIHQVCSPSVARALRSRCVAAVPSRNLSTSISPSAEPSPEAPKVLIGLSDQEL 60

Query: 61  QQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120
           QQLALDFGQEKYRGK LHQLIYKRK+KEIEEFTNLP  FRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKLLHQLIYKRKVKEIEEFTNLPLKFRNDLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           A+DGTVKLLLKLDDNRLIETVGIPVEDK+NKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 AADGTVKLLLKLDDNRLIETVGIPVEDKNNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+MKDC DYFL+
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEALMKDCHDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWG GYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMAC 418
           AFVAALES KITVSVR+TRGLDANAACGQLRNQFQK+PL+ DSD ++S E+A A+AC
Sbjct: 361 AFVAALESHKITVSVRRTRGLDANAACGQLRNQFQKNPLLVDSDISQS-EAAEAVAC 416

BLAST of MS017289 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 606.7 bits (1563), Expect = 1.4e-173
Identity = 309/422 (73.22%), Postives = 356/422 (84.36%), Query Frame = 0

Query: 5   SMALIHH-VCSTSAARAVRPRRVAAVPSRNLSTSISPRVRP--------PPEASKVLIGL 64
           +MA++ + V S   +R V  R   A      +T++ P  +P        P   S VLIG+
Sbjct: 8   TMAMLQNLVFSVPISRMVVRRHSLATTFSAAATTVVPSPKPVSAKPARTPHVDSHVLIGM 67

Query: 65  SEQELQQLALDFGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGRSPV 124
           SE ELQ+LA++ GQE YRGKQLH LIYKRK+ ++E+F+NLP  FR GL + G++VGRSP+
Sbjct: 68  SEPELQELAINLGQEGYRGKQLHHLIYKRKVNKVEDFSNLPLTFRKGLVDGGFKVGRSPI 127

Query: 125 YQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCAT 184
           YQ+VTA+DGT+KLLLKL+DN LIETVGIPV+D D KG  RLTAC+SSQVGCPLRCSFCAT
Sbjct: 128 YQTVTATDGTIKLLLKLEDNLLIETVGIPVQD-DEKGITRLTACVSSQVGCPLRCSFCAT 187

Query: 185 GKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQI 244
           GKGGFSRNLQRHEI+EQV AIED+F HRVTNVVFMGMGEPMLN+KSVL+AH+CLNKD++I
Sbjct: 188 GKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGEPMLNLKSVLDAHRCLNKDIEI 247

Query: 245 GQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCR 304
           GQRMITISTVGVPNTIK+LASHKLQSTLAVSLHAPNQ LRE IVPSAKAYPLEAIMKDCR
Sbjct: 248 GQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMKDCR 307

Query: 305 DYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPY 364
           DYF ET+RRVSFEYALLAGVND VEHAVELA+LL EWG+ YHVNLIP+NPIEGSEYQRPY
Sbjct: 308 DYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQRPY 367

Query: 365 KKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAM 418
           KKAV+AF AALESRKIT SVRQTRGLDA+AACGQLRN+FQK PL+ ++DS  S   A A+
Sbjct: 368 KKAVLAFAAALESRKITASVRQTRGLDASAACGQLRNKFQKSPLLTETDSQESQPDAEAV 427

BLAST of MS017289 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 597.8 bits (1540), Expect = 6.6e-171
Identity = 308/425 (72.47%), Postives = 355/425 (83.53%), Query Frame = 0

Query: 5   SMALIHH-VCSTSAARAVRPRRVAAVPSRNLSTSISPRVRP--------PPEASKVLIGL 64
           +MA++ + V S   +R V  R   A      +T++ P  +P        P   S VLIG+
Sbjct: 8   TMAMLQNLVFSVPISRMVVRRHSLATTFSAAATTVVPSPKPVSAKPARTPHVDSHVLIGM 67

Query: 65  SEQELQQLALD---FGQEKYRGKQLHQLIYKRKLKEIEEFTNLPQAFRNGLQEAGWRVGR 124
           SE ELQ+LA++     QE YRGKQLH LIYKRK+ ++E+F+NLP  FR GL + G++VGR
Sbjct: 68  SEPELQELAINLVLIFQEGYRGKQLHHLIYKRKVNKVEDFSNLPLTFRKGLVDGGFKVGR 127

Query: 125 SPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSF 184
           SP+YQ+VTA+DGT+KLLLKL+DN LIETVGIPV+D D KG  RLTAC+SSQVGCPLRCSF
Sbjct: 128 SPIYQTVTATDGTIKLLLKLEDNLLIETVGIPVQD-DEKGITRLTACVSSQVGCPLRCSF 187

Query: 185 CATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKD 244
           CATGKGGFSRNLQRHEI+EQV AIED+F HRVTNVVFMGMGEPMLN+KSVL+AH+CLNKD
Sbjct: 188 CATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGEPMLNLKSVLDAHRCLNKD 247

Query: 245 VQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMK 304
           ++IGQRMITISTVGVPNTIK+LASHKLQSTLAVSLHAPNQ LRE IVPSAKAYPLEAIMK
Sbjct: 248 IEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMK 307

Query: 305 DCRDYFLETSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQ 364
           DCRDYF ET+RRVSFEYALLAGVND VEHAVELA+LL EWG+ YHVNLIP+NPIEGSEYQ
Sbjct: 308 DCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQ 367

Query: 365 RPYKKAVVAFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESA 418
           RPYKKAV+AF AALESRKIT SVRQTRGLDA+AACGQLRN+FQK PL+ ++DS  S   A
Sbjct: 368 RPYKKAVLAFAAALESRKITASVRQTRGLDASAACGQLRNKFQKSPLLTETDSQESQPDA 427

BLAST of MS017289 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-48
Identity = 122/312 (39.10%), Postives = 181/312 (58.01%), Query Frame = 0

Query: 90  EEFTNLPQAFRNGLQEAGWRVGRSPVYQSVTASDGTV-KLLLKLDDNRLIETVGIPVE-- 149
           +E  +LP A    L  + ++   S ++    +SDGT  KLL+KL +   +E V +  +  
Sbjct: 42  DEIPSLPSA-AYSLLHSKFKTLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTR 101

Query: 150 -----DKDNKGSVRLTACISSQVGCPLRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFN 209
                 K   G +R T CISSQVGC + C+FCATG  GF  NL   EIVEQ+     I +
Sbjct: 102 LGMLGGKPRPGGIRSTLCISSQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIAD 161

Query: 210 HRVTNVVFMGMGEPMLNMKSVLEA-HQCLNKDVQIGQRMITISTVGVPNTIKRLASHKLQ 269
             + N+VFMGMGEP+ N  +V+EA    LN+  Q+  + ITISTVG+ + I +L +    
Sbjct: 162 --IRNIVFMGMGEPLNNYNAVVEAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPG 221

Query: 270 STLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLETSRRVSFEYALLAGVNDAVE 329
            +LAVSLHAP Q++R  I+P+A+A+PL+ +M   + +   + +++  EY +L GVND  +
Sbjct: 222 VSLAVSLHAPVQEIRCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQ 281

Query: 330 HAVELAKLLHEWGRGYHVNLIPFNPI-EGSEYQRPYKKAVVAFVAAL-ESRKITVSVRQT 389
           HA  L +LL  +     +NLIPFNPI   S+++    + V  F   L E+ KI  ++R+ 
Sbjct: 282 HAHLLGELLKTF--QVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKE 341

Query: 390 RGLDANAACGQL 391
            G D + ACGQL
Sbjct: 342 MGQDISGACGQL 348

BLAST of MS017289 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-45
Identity = 129/372 (34.68%), Postives = 190/372 (51.08%), Query Frame = 0

Query: 27  AAVPSRNLSTSISPRVRPPPEASKVLIGLSEQELQQLALDFGQEKYRGKQLHQLIYKRKL 86
           A +PS     S    V+       VL G++   LQ+     G    +   L + +YK  +
Sbjct: 72  AYLPSLEEYPSAKGSVKRDDNQKVVLKGMTYASLQEWVQSHGFRPGQALMLWKRLYKDNI 131

Query: 87  --KEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIP 146
               ++E   L +  +  + E     G        +ASDGT K+L  LDD  +IETV IP
Sbjct: 132 WANNVDELEGLNKDLKRMISEHA-EFGALSFKDIRSASDGTRKILFTLDDGLVIETVVIP 191

Query: 147 VEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRV 206
            +    +G  R T C+SSQVGC + C FC TG+ G  RNL   EIVEQ      + +H V
Sbjct: 192 CD----RG--RTTVCVSSQVGCAMNCQFCYTGRMGLKRNLTTAEIVEQAVYARRLLSHEV 251

Query: 207 ---TNVVFMGMGEPMLNMKSVLEAHQCL--NKDVQIGQRMITISTVGVPNTIKRLASHKL 266
              TNVVFMGMGEP  N+ +V++A   +     +    R +T+ST G+   +KR    + 
Sbjct: 252 GSITNVVFMGMGEPFHNIDNVIKAANIMVDENGLHFSPRKVTVSTSGLVPQLKRFL-RES 311

Query: 267 QSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLETSR-RVSFEYALLAGVNDA 326
              LAVSL+A   ++R  I+P  + Y L  +++  R+      + +V FEY +LAGVND+
Sbjct: 312 NCALAVSLNATTDEVRNWIMPINRKYKLSLLLETLREGLSSRHKYKVLFEYVMLAGVNDS 371

Query: 327 VEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVVAFVAALESRKITVSVRQT 386
           ++ A  L +L+   G    +NLI FNP  GS++ +  +  ++ F   L     TV +R +
Sbjct: 372 MDDARRLVELVQ--GIPCKINLIQFNPHSGSQFIQTEEDKMIKFRNVLAEGGCTVLMRFS 431

Query: 387 RGLDANAACGQL 391
           RG D  AACGQL
Sbjct: 432 RGNDQMAACGQL 433

BLAST of MS017289 vs. TAIR 10
Match: AT1G50050.1 (CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 2.2e-09
Identity = 30/41 (73.17%), Postives = 36/41 (87.80%), Query Frame = 0

Query: 364 AALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSD 405
           AAL+SRKIT SVRQTRGL A+AACGQLRN+FQK P +A ++
Sbjct: 154 AALKSRKITASVRQTRGLGASAACGQLRNKFQKSPSLATAE 194

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139334.12.6e-23099.04uncharacterized protein LOC111010274 [Momordica charantia][more]
XP_038892136.11.9e-21292.09probable dual-specificity RNA methyltransferase RlmN [Benincasa hispida][more]
XP_022957587.11.4e-21091.13uncharacterized protein LOC111458934 [Cucurbita moschata][more]
XP_022989725.11.4e-21091.37uncharacterized protein LOC111486721 [Cucurbita maxima][more]
KAG7031617.15.2e-21091.13rlmN, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q2JRQ82.7e-10055.20Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strai... [more]
Q2JMN28.6e-9954.34Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strai... [more]
B0C9F45.6e-9853.13Probable dual-specificity RNA methyltransferase RlmN OS=Acaryochloris marina (st... [more]
Q8DG981.6e-9754.25Probable dual-specificity RNA methyltransferase RlmN OS=Thermosynechococcus elon... [more]
Q7U8K04.0e-9652.42Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strai... [more]
Match NameE-valueIdentityDescription
A0A6J1CCN01.3e-23099.04uncharacterized protein LOC111010274 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1JN656.6e-21191.37uncharacterized protein LOC111486721 OS=Cucurbita maxima OX=3661 GN=LOC111486721... [more]
A0A6J1GZJ06.6e-21191.13uncharacterized protein LOC111458934 OS=Cucurbita moschata OX=3662 GN=LOC1114589... [more]
A0A0A0KLM93.3e-21089.41Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G181470... [more]
A0A1S3CGI77.3e-21091.13probable dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=... [more]
Match NameE-valueIdentityDescription
AT2G39670.11.4e-17373.22Radical SAM superfamily protein [more]
AT2G39670.26.6e-17172.47Radical SAM superfamily protein [more]
AT3G19630.12.4e-4839.10Radical SAM superfamily protein [more]
AT1G60230.11.9e-4534.68Radical SAM superfamily protein [more]
AT1G50050.12.2e-0973.17CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 pro... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 156..374
e-value: 0.0059
score: 7.6
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 161..325
e-value: 1.1E-14
score: 55.1
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 152..382
score: 36.012527
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 113..399
e-value: 2.2E-109
score: 367.0
NoneNo IPR availableGENE3D1.10.150.530coord: 50..106
e-value: 4.0E-14
score: 53.9
NoneNo IPR availableSFLDSFLDG01062methyltransferase_(Class_A)coord: 48..396
e-value: 0.0
score: 412.0
NoneNo IPR availablePANTHERPTHR30544:SF5RADICAL SAM SUPERFAMILY PROTEINcoord: 36..405
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 165..358
e-value: 1.20853E-11
score: 61.5805
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 129..355
IPR004383Ribosomal RNA large subunit methyltransferase RlmN/CfrPIRSFPIRSF006004Cfrcoord: 41..412
e-value: 4.2E-131
score: 434.8
IPR004383Ribosomal RNA large subunit methyltransferase RlmN/CfrSFLDSFLDF00275adenosine_C2_methyltransferascoord: 48..396
e-value: 0.0
score: 412.0
IPR027492Dual-specificity RNA methyltransferase RlmNTIGRFAMTIGR00048TIGR00048coord: 51..396
e-value: 6.4E-120
score: 398.3
IPR027492Dual-specificity RNA methyltransferase RlmNHAMAPMF_01849RNA_methyltr_RlmNcoord: 49..396
score: 63.449818
IPR040072Methyltransferase (Class A)PANTHERPTHR3054423S RRNA METHYLTRANSFERASEcoord: 36..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017289.1MS017289.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
biological_process GO:0006364 rRNA processing
biological_process GO:0030488 tRNA methylation
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0008173 RNA methyltransferase activity