MS017252 (gene) Bitter gourd (TR) v1

BLAST of MS017252 vs. NCBI nr
Match: XP_022139190.1 (LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia])

HSP 1 Score: 1434.5 bits (3712), Expect = 0.0e+00
Identity = 726/741 (97.98%), Postives = 728/741 (98.25%), Query Frame = 0

Query: 1   TMSSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIG 60
           TMSSLIFKFIFFSLFYRLLVSGSH DNDGRKTYIVYMGSKPEDTTSTP HHRAMLEEVIG
Sbjct: 6   TMSSLIFKFIFFSLFYRLLVSGSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAMLEEVIG 65

Query: 61  STF---APESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM 120
           STF   APESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM
Sbjct: 66  STFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM 125

Query: 121 GFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKI 180
           GFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKI
Sbjct: 126 GFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKI 185

Query: 181 IGARVYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRI 240
           IGARVYRSESLPP DIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPS RI
Sbjct: 186 IGARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSARI 245

Query: 241 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGI 300
           AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEP SYFSDPIAIGAFHAMKHGI
Sbjct: 246 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMKHGI 305

Query: 301 LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLG 360
           LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIY QGVAINTFDLLG
Sbjct: 306 LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIY-QGVAINTFDLLG 365

Query: 361 KQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVG 420
           KQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVG
Sbjct: 366 KQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVG 425

Query: 421 IVMRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVS 480
           IVM+DLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVS
Sbjct: 426 IVMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVS 485

Query: 481 FSSRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHA 540
           FSSRGP FITRDILK DLSAPGVEILAAWSPLAPV GVKGDSRSELYNIISGTSMSCPHA
Sbjct: 486 FSSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHA 545

Query: 541 TAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYD 600
           TAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYD
Sbjct: 546 TAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYD 605

Query: 601 ANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF 660
           ANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF
Sbjct: 606 ANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF 665

Query: 661 RRTLTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSA 720
           RRTLTNVESGASTYSAVVFGPPDGL+ITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSA
Sbjct: 666 RRTLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSA 725

Query: 721 SLVWSDGVHQVRSPITIYFLK 739
           SL WSDGVHQVRSPITIYFLK
Sbjct: 726 SLEWSDGVHQVRSPITIYFLK 745

BLAST of MS017252 vs. NCBI nr
Match: XP_023548142.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 568/735 (77.28%), Postives = 636/735 (86.53%), Query Frame = 0

Query: 3   SSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGST 62
           SSL+F FIF +LF  LLVS SH D+DGRKTYIVYMGSKPED +ST  +HR MLE+V+GS+
Sbjct: 8   SSLVFNFIFLNLFSCLLVSSSHSDSDGRKTYIVYMGSKPEDISSTSLYHRTMLEQVVGSS 67

Query: 63  FAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQS 122
           F+P+ LLYSYKRSFNGFAVRLT+EEA K+A +EGVVSVFPN KKH+HTTRSWDFMGF+QS
Sbjct: 68  FSPKHLLYSYKRSFNGFAVRLTKEEAQKIALEEGVVSVFPNEKKHVHTTRSWDFMGFTQS 127

Query: 123 VPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARV 182
           VPR +QVES++VVGVLDTGIWPESPSF DE   PPP +W G CQ + DF+CN+KIIGAR 
Sbjct: 128 VPRVNQVESNIVVGVLDTGIWPESPSFNDEGVDPPPKEWKGTCQISPDFQCNRKIIGARA 187

Query: 183 YRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYKI 242
           YRSE+LP G++ +PRDS GHGTHTAS VAGGLV +ASLYGLG GTARGGVPS RIAVYKI
Sbjct: 188 YRSENLPKGEVVNPRDSKGHGTHTASIVAGGLVRKASLYGLGFGTARGGVPSARIAVYKI 247

Query: 243 CWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSNS 302
           CWSDGC DADILAAFDDAIADGVDIISLSVGGS+PKSYF+D IAIGAFHAMKHGILTSNS
Sbjct: 248 CWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKSYFNDSIAIGAFHAMKHGILTSNS 307

Query: 303 AGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYPL 362
           AGN GPNYFT SN+SPWSLSVAAST DRK ++ VQ GNG ++ QGVAINTFDLL KQYP+
Sbjct: 308 AGNRGPNYFTTSNVSPWSLSVAASTMDRKFVSNVQFGNGTLH-QGVAINTFDLLEKQYPM 367

Query: 363 IYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMRD 422
           I+ GDAPN A GFN S SR CVENSVD +LVRGKILVCDSLL A+ + SF   VGI+M+ 
Sbjct: 368 IHGGDAPNKARGFNASTSRFCVENSVDLSLVRGKILVCDSLLRAATMPSFTGAVGIIMQG 427

Query: 423 LRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSRG 482
            R KDYASSYP PA+YL  AA +DIQL  SS T+ TATIL+S AV+DASAP VVSFSSRG
Sbjct: 428 TREKDYASSYPLPATYLHIAAANDIQL--SSPTESTATILQSNAVKDASAPSVVSFSSRG 487

Query: 483 PNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIAA 542
           PN +T DILKPDL+APGVEILAAWSP+APVSGV GDSRS +YNIISGTSMSCPHATA+A 
Sbjct: 488 PNLVTLDILKPDLTAPGVEILAAWSPIAPVSGVVGDSRSVVYNIISGTSMSCPHATAMAV 547

Query: 543 YVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANEGD 602
           YVKTFHPTWSPAA+KSALMTTAFTMN+KLN QAEFAYG+GHVNPLKA+NPGLVY+A+E D
Sbjct: 548 YVKTFHPTWSPAAIKSALMTTAFTMNSKLNTQAEFAYGAGHVNPLKAINPGLVYNASESD 607

Query: 603 YVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRRTLT 662
           Y+  LCGQGY+TAMVR IT DSSACT  N GRVWDLNYPSFALST PS  I+QFF RTLT
Sbjct: 608 YIDMLCGQGYTTAMVRLITHDSSACTSTNTGRVWDLNYPSFALSTTPSESIHQFFTRTLT 667

Query: 663 NVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLVWS 722
           NVE  AS Y+A VF PP  L ITV+P+VLSFNG+G+TKSF LTVQGTVSQAIVSASLVWS
Sbjct: 668 NVELRASIYTATVFAPP-SLRITVDPAVLSFNGIGETKSFRLTVQGTVSQAIVSASLVWS 727

Query: 723 DGVHQVRSPITIYFL 738
           DG+H VRSPI +Y +
Sbjct: 728 DGMHDVRSPIIVYVI 738

BLAST of MS017252 vs. NCBI nr
Match: XP_038891007.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 572/737 (77.61%), Postives = 627/737 (85.07%), Query Frame = 0

Query: 2   MSSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGS 61
           +SSLI  FIF +LF   LVS SH DND RKTYIVYMG K EDT+STP HHR MLE+V+GS
Sbjct: 6   LSSLILNFIFLNLFNCQLVSSSHSDNDDRKTYIVYMGRKLEDTSSTPLHHRTMLEQVVGS 65

Query: 62  TFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQ 121
           TFAPE LLYSYKRSFNGFAV+LT+EEA K+A KEGVVSVFPNGKKH+HTTRSWDFMGF+Q
Sbjct: 66  TFAPEHLLYSYKRSFNGFAVKLTKEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQ 125

Query: 122 SVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGAR 181
           SVP   QVES++VVGVLDTGIWPESPSF D    PPPA W G CQ +  F+CN+KIIGAR
Sbjct: 126 SVPHVKQVESNIVVGVLDTGIWPESPSFNDADLDPPPAGWKGECQISPTFQCNRKIIGAR 185

Query: 182 VYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYK 241
           +YRSE+  P DI+SPRDS GHGTHTASTVAGGLV QASLYGLG GTARGGVPS RIAVYK
Sbjct: 186 LYRSENFSPEDIQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYK 245

Query: 242 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSN 301
           ICWSDGC DADILAAFDDAIADGVDIISLSVGGSEPKSYF+D IAIGAFHAM+HGILTSN
Sbjct: 246 ICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKSYFNDSIAIGAFHAMQHGILTSN 305

Query: 302 SAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYP 361
           SAGNEGP YFT SNISPWSLSVAAST DRK ++QVQL NG IY QGVAI+TFDL+GKQ+P
Sbjct: 306 SAGNEGPEYFTTSNISPWSLSVAASTMDRKFVSQVQLANGTIY-QGVAIHTFDLMGKQHP 365

Query: 362 LIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASF--NATVGIV 421
           LIY GDAPN AGGFN S SR C E SVD +LV+GKILVCDSLL AS + SF  N  VGI+
Sbjct: 366 LIYGGDAPNKAGGFNSSTSRFCDEKSVDPSLVKGKILVCDSLLRASTMESFNNNGVVGII 425

Query: 422 MRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFS 481
           M+  RLKDYASSYP PASYL     +DI+L        TATI KS A  DASAP VVSFS
Sbjct: 426 MQGSRLKDYASSYPLPASYLH---NEDIKL------SSTATIFKSNANLDASAPSVVSFS 485

Query: 482 SRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATA 541
           SRGPN +T DILKPDL+APGVEILAAWSP+APVSG   D+RS LYNIISGTSMSCPHATA
Sbjct: 486 SRGPNLVTLDILKPDLTAPGVEILAAWSPIAPVSGTARDTRSVLYNIISGTSMSCPHATA 545

Query: 542 IAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDAN 601
           IA YVKTFHPTWSPAA+KSALMTTAF+MN K+NPQAEFAYG+GH+NPLKA+NPGLVY+A+
Sbjct: 546 IAVYVKTFHPTWSPAAIKSALMTTAFSMNGKVNPQAEFAYGAGHINPLKAINPGLVYNAS 605

Query: 602 EGDYVKFLCG-QGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFR 661
           E DY+KFLCG +GY+T MVR ITGD++ACTP N GRVWDLNYPSFALST PS+ INQFF 
Sbjct: 606 ETDYIKFLCGHEGYTTDMVRHITGDNTACTPTNSGRVWDLNYPSFALSTTPSQSINQFFT 665

Query: 662 RTLTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSAS 721
           RTLTNVE   S YSA+VF PP  L ITV P +LSFNG+GDTKSF LTVQGTVSQAIVSAS
Sbjct: 666 RTLTNVEFRTSLYSAMVFAPP-SLRITVEPPLLSFNGIGDTKSFKLTVQGTVSQAIVSAS 725

Query: 722 LVWSDGVHQVRSPITIY 736
           LVW+DGVHQVRSPIT+Y
Sbjct: 726 LVWTDGVHQVRSPITVY 731

BLAST of MS017252 vs. NCBI nr
Match: XP_008461717.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 566/738 (76.69%), Postives = 622/738 (84.28%), Query Frame = 0

Query: 5   LIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGSTFA 64
           LI  F+F +LF    V GSH DNDGRKTYIVYMGS  EDT+STP HHRAMLE+VIGS FA
Sbjct: 12  LILNFVFLNLFNCQPVFGSHLDNDGRKTYIVYMGSTLEDTSSTPLHHRAMLEQVIGSNFA 71

Query: 65  PESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQSVP 124
           P+ LLYSYKRSFNGFAVRLTEEEA K+A KEGVVSVFPNGKKH+HTTRSWDFMGF+QSVP
Sbjct: 72  PKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVP 131

Query: 125 RAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARVYR 184
           R +QVES++VVGVLDTGIWPESPSF D    PPPA W G CQ + DF+CN+KIIGAR YR
Sbjct: 132 RVNQVESNIVVGVLDTGIWPESPSFNDADLDPPPAGWKGQCQTSPDFQCNRKIIGARTYR 191

Query: 185 SESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYKICW 244
           SE+LPPG+ +SPRDS GHGTHTASTVAGGLV QASLYGLG GTARGGVPS RIAVYKICW
Sbjct: 192 SENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYKICW 251

Query: 245 SDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSNSAG 304
           SDGC DADILAAFDDAIADGVDIISLSVGGS  KSYF+D IAIGAFHAMKHGILTSNSAG
Sbjct: 252 SDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMKHGILTSNSAG 311

Query: 305 NEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYPLIY 364
           NEGP YFT SN+SPWSLSVAAST DRK +++VQL NG  Y QG AI+TFDL+GKQYPLIY
Sbjct: 312 NEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAY-QGPAIHTFDLMGKQYPLIY 371

Query: 365 AGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASF--NATVGIVMRD 424
            GDAPN +GGFN SISR C ENSVD++LV+GKILVCDS+L AS + SF  N  VGI+M+ 
Sbjct: 372 GGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKNGAVGIIMQG 431

Query: 425 LRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSRG 484
            R KDYASSYP PASYL  A         + T   TA+I KS A+ D+SAP VVSFSSRG
Sbjct: 432 SRFKDYASSYPLPASYLHNA---------NITLSSTASIFKSNAILDSSAPSVVSFSSRG 491

Query: 485 PNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIAA 544
           PN  T DILKPDL+APGVEILAAWSP+A VSGV GDSRS LYNIISGTSMSCPHATAIA 
Sbjct: 492 PNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAV 551

Query: 545 YVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANEGD 604
           YVKTF+PTWSPAA+KSALMTTAF+MNAK+NPQAEFAYG+GH+NPLKAVNPGLVY+A E D
Sbjct: 552 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETD 611

Query: 605 YVKFLCGQ-GYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSR-PINQFFRRT 664
           Y+ FLCGQ GY+T MVRRITGD++ACTP N GRVWDLNYPSFA ST PS+  INQFF RT
Sbjct: 612 YINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFART 671

Query: 665 LTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLV 724
           LTNVES  S YSA VF PP  L ITV+P  L FNG+GDTKSF LTVQGTV+Q I+S SLV
Sbjct: 672 LTNVESKTSLYSAKVFAPP-SLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLV 731

Query: 725 WSDGVHQVRSPITIYFLK 739
           W+DGVHQVRSPIT+Y  K
Sbjct: 732 WTDGVHQVRSPITVYVAK 738

BLAST of MS017252 vs. NCBI nr
Match: XP_022138973.1 (cucumisin-like [Momordica charantia])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 553/735 (75.24%), Postives = 622/735 (84.63%), Query Frame = 0

Query: 3   SSLIFKFIFFSLFYRLLVSGSHFDN-DGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGS 62
           SSLIFK +F SL   LL S    +N DGRK YIVY+G+KPEDT STPSHH  MLEEV+GS
Sbjct: 3   SSLIFKLVFLSLLCGLLASSLDSNNDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVVGS 62

Query: 63  TFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQ 122
           TFAPE+LL+SYKRSFNGF V+LTEEEA K+AAKEGVVSVF NGKKHLHTTRSWDFMGF++
Sbjct: 63  TFAPEALLHSYKRSFNGFVVKLTEEEAQKIAAKEGVVSVFLNGKKHLHTTRSWDFMGFTK 122

Query: 123 SVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGAR 182
           +V R  QVES++VVGVLD+GIWPESPSF D  +GPPP KW G CQ + +F CNKKIIGAR
Sbjct: 123 NVARVKQVESNIVVGVLDSGIWPESPSFSDLGYGPPPVKWKGTCQTSANFHCNKKIIGAR 182

Query: 183 VYRSES-LPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVY 242
            YRS +  PP DIRSPRDS+GHGTHTASTVAGGLV QASLYGL +GTARGGVPS RIAVY
Sbjct: 183 AYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 242

Query: 243 KICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTS 302
           KICWSDGC DADILAAFDDAIADGVDIISLSVGGSEPK YF+D IAIGAFH+MKHGILTS
Sbjct: 243 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 302

Query: 303 NSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQY 362
           NSAGN+GP+YFT  N SPWSLSVAAS+ DRK +T+VQLGN N+Y QG  INTFDLLGKQY
Sbjct: 303 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLY-QGYTINTFDLLGKQY 362

Query: 363 PLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVM 422
           PLIY GDAPNI GGF  S SR C +N+VD+NLV+GKILVCD++L  S  ASF   VG+VM
Sbjct: 363 PLIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASFXGAVGVVM 422

Query: 423 RDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSS 482
           +D  +KD A SYP PASYL TAA   I+ Y++S    TATILKS AV D SAP+VVSFSS
Sbjct: 423 QDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSFSS 482

Query: 483 RGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAI 542
           RGPN  T DILKPDL+APGVEILAAWSP+AP+SG+  DSR  LYNIISGTSMSCPHATA+
Sbjct: 483 RGPNPETFDILKPDLTAPGVEILAAWSPIAPISGIT-DSRKSLYNIISGTSMSCPHATAV 542

Query: 543 AAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANE 602
           A YVKTFHPTWSPAA+KSALMTTA  +N KLNPQAEFAYG+GHVNPLKAVNPGLVYDA+E
Sbjct: 543 AVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDAHE 602

Query: 603 GDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRRT 662
            DYVKFLCGQGY+TAMV+R+T D+SACTP N  RVWDLNYPSFALS+ PS  INQFF RT
Sbjct: 603 SDYVKFLCGQGYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSESINQFFTRT 662

Query: 663 LTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLV 722
           LTNV S ASTY A V+G P GL I+V+P VLSF+ +G+ KSF LT++GT+S +IVSASLV
Sbjct: 663 LTNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTLSHSIVSASLV 722

Query: 723 WSDGVHQVRSPITIY 736
           W+DG+HQVRSPIT+Y
Sbjct: 723 WNDGLHQVRSPITVY 735

BLAST of MS017252 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 6.3e-278
Identity = 486/735 (66.12%), Postives = 572/735 (77.82%), Query Frame = 0

Query: 3   SSLIFKFIFFSLFY-RLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGS 62
           SSLIFK  FFSLF+   L S    D+DG+  YIVYMG K ED  S   HHRAMLE+V+GS
Sbjct: 3   SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGS 62

Query: 63  TFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQ 122
           TFAPES+L++YKRSFNGFAV+LTEEEA K+A+ EGVVSVF N    LHTTRSWDF+GF  
Sbjct: 63  TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPL 122

Query: 123 SVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGAR 182
           +VPR  QVES++VVGVLDTGIWPESPSF DE F PPP KW G C+ +++FRCN+KIIGAR
Sbjct: 123 TVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGAR 182

Query: 183 VYR-SESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVY 242
            Y     + PGD+  PRD+NGHGTHTAST AGGLV QA+LYGLG+GTARGGVP  RIA Y
Sbjct: 183 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 242

Query: 243 KICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTS 302
           K+CW+DGCSD DILAA+DDAIADGVDIISLSVGG+ P+ YF D IAIG+FHA++ GILTS
Sbjct: 243 KVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 302

Query: 303 NSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQY 362
           NSAGN GPN+FT +++SPW LSVAAST DRK +TQVQ+GNG  + QGV+INTFD   + Y
Sbjct: 303 NSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSF-QGVSINTFD--NQYY 362

Query: 363 PLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASK-LASFNATVGIV 422
           PL+   D PN   GF+ S SR C + SV+ NL++GKI+VC++  G  +   S +   G++
Sbjct: 363 PLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVL 422

Query: 423 MRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFS 482
           M     +DYA SYP P+S L          YI S   P ATI KS  + +ASAPVVVSFS
Sbjct: 423 MTS-NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFS 482

Query: 483 SRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATA 542
           SRGPN  T+D++KPD+S PGVEILAAW  +APV G++   R+ L+NIISGTSMSCPH T 
Sbjct: 483 SRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR---RNTLFNIISGTSMSCPHITG 542

Query: 543 IAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDAN 602
           IA YVKT++PTWSPAA+KSALMTTA  MNA+ NPQAEFAYGSGHVNPLKAV PGLVYDAN
Sbjct: 543 IATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDAN 602

Query: 603 EGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRR 662
           E DYVKFLCGQGY+T  VRRITGD SACT GN GRVWDLNYPSF LS +PS+  NQ+F R
Sbjct: 603 ESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNR 662

Query: 663 TLTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASL 722
           TLT+V   ASTY A++   P GL I+VNP+VLSFNG+GD KSF LTV+G++   +VSASL
Sbjct: 663 TLTSVAPQASTYRAMI-SAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASL 722

Query: 723 VWSDGVHQVRSPITI 735
           VWSDGVH VRSPITI
Sbjct: 723 VWSDGVHYVRSPITI 727

BLAST of MS017252 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 1.8e-187
Identity = 346/719 (48.12%), Postives = 468/719 (65.09%), Query Frame = 0

Query: 27  NDGRK---TYIVYMGSKPEDTTSTPSHHRAMLEEVIGSTFAPESLLYSYKRSFNGFAVRL 86
           ND R+    YIVYMG+ PE   S PSHH ++L++++G+  A   L+ SYKRSFNGFA  L
Sbjct: 24  NDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANL 83

Query: 87  TEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQSVPRAHQVESDVVVGVLDTGIW 146
           ++ E+ K+   + VVSVFP+    L TTRSWDF+GF +   R    ESDV+VGV+D+GIW
Sbjct: 84  SQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIW 143

Query: 147 PESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARVYRSESLPPGDIRSPRDSNGHG 206
           PES SF DE FGPPP KW G+C+    F CN K+IGAR Y   +       S RD  GHG
Sbjct: 144 PESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFA------DSARDEEGHG 203

Query: 207 THTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYKICWSDGCSDADILAAFDDAIAD 266
           THTAST AG  V+ AS YGL  GTARGGVPS RIA YK+C+ + C+D DILAAFDDAIAD
Sbjct: 204 THTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIAD 263

Query: 267 GVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSNSAGNEGPNYFTASNISPWSLSV 326
           GVD+IS+S+      +  +  +AIG+FHAM  GI+T+ SAGN GP+  + +N+SPW ++V
Sbjct: 264 GVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITV 323

Query: 327 AASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYPLIYAGDAPNIAGGFNGSISRLC 386
           AAS TDR+ + +V LGNG     G+++NTF+L G ++P++Y     N++   + + +  C
Sbjct: 324 AASGTDRQFIDRVVLGNGKAL-TGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYC 383

Query: 387 VENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMRDLRLKDYASSYPFPASYLRTAA 446
               VD  LV+GKI++CD  LG  + A     +G+++++  L D A   PFPAS L    
Sbjct: 384 SSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFED 443

Query: 447 TDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSRGPNFITRDILKPDLSAPGVEIL 506
              I+ YI S   P A IL++  + D  AP V SFSSRGP+F+ +++LKPD+SAPG+EIL
Sbjct: 444 YKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEIL 503

Query: 507 AAWSPLAPVSGVKG--DSRSELYNIISGTSMSCPHATAIAAYVKTFHPTWSPAALKSALM 566
           AA+SP+A  S      D RS  Y+++SGTSM+CPH   +AAYVK+FHP WSP+A+KSA+M
Sbjct: 504 AAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIM 563

Query: 567 TTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANEGDYVKFLCGQGYSTAMVRRIT 626
           TTA  MN K NP+ EFAYGSG +NP KA +PGLVY+    DY+K LC +G+ +  +   +
Sbjct: 564 TTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTS 623

Query: 627 GDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRRTLTNVESGASTYSAVVFGPPDG 686
           G +  C+      V DLNYP+     +   P N  F+RT+TNV    STY A V      
Sbjct: 624 GQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPE 683

Query: 687 LNITVNPSVLSFNGVGDTKSFNLTVQGTVSQ--AIVSASLVWSDGVHQVRSPITIYFLK 739
           L I++ P +L F  + + KSF +T+ G   +  + VS+S+VWSDG H VRSPI  Y ++
Sbjct: 684 LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSIQ 728

BLAST of MS017252 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 2.9e-182
Identity = 355/743 (47.78%), Postives = 472/743 (63.53%), Query Frame = 0

Query: 2   MSSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEEVIG 61
           M++L       S    L +S      D ++ YIVYMGS       TP S H  +L+EV G
Sbjct: 1   MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60

Query: 62  STFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFS 121
            +     L+ SYKRSFNGFA RLTE E  +VA   GVVSVFPN K  L TT SWDFMG  
Sbjct: 61  ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120

Query: 122 QSV--PRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKII 181
           + +   R   VESD ++GV+D+GI PES SF D+ FGPPP KW G C    +F CN K+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180

Query: 182 GARVYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIA 241
           GAR Y SE          RD +GHGTHTAST AG  V  AS +G+G GT RGGVP++R+A
Sbjct: 181 GARDYTSE--------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVA 240

Query: 242 VYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGIL 301
            YK+C   GCS   +L+AFDDAIADGVD+I++S+G      + +DPIAIGAFHAM  G+L
Sbjct: 241 AYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVL 300

Query: 302 TSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGK 361
           T NSAGN GP   + S ++PW L+VAASTT+R  +T+V LGNG     G ++N +++ GK
Sbjct: 301 TVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTL-VGKSVNAYEMKGK 360

Query: 362 QYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGI 421
            YPL+Y   A + A   +   + LC  + VDK+ V+GKILVC    G  K+      VG+
Sbjct: 361 DYPLVYGKSAASSA--CDAESAGLCELSCVDKSRVKGKILVCGG-PGGLKIVESVGAVGL 420

Query: 422 VMRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSF 481
           + R  +  D A  +P PA+ L T   + +  Y+ ST  P A +LK+ A+ + ++PV+ SF
Sbjct: 421 IYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASF 480

Query: 482 SSRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHAT 541
           SSRGPN I  DILKPD++APGVEILAA+SP    S  + D+R   Y+++SGTSMSCPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVA 540

Query: 542 AIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLN--PQAEFAYGSGHVNPLKAVNPGLVY 601
            +AAYVKTF+P WSP+ ++SA+MTTA+ +NA        EFAYGSGHV+P+ A NPGLVY
Sbjct: 541 GVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVY 600

Query: 602 DANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPS-RPINQ 661
           + ++ D++ FLCG  Y++ +++ I+G++  C+        +LNYPS +   + S      
Sbjct: 601 ELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTV 660

Query: 662 FFRRTLTNVESGASTY-SAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGT--VSQ 721
            F RTLTNV +  STY S VV G    L++ + PSVLSF  V + +SF +TV G+   S+
Sbjct: 661 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 720

Query: 722 AIVSASLVWSDGVHQVRSPITIY 736
              SA+L+WSDG H VRSPI +Y
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVY 728

BLAST of MS017252 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 2.5e-181
Identity = 349/741 (47.10%), Postives = 480/741 (64.78%), Query Frame = 0

Query: 7   FKFIFFSLFYRLL--VSGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEEVIGSTF 66
           F F+F SL    L  VS    D+  ++ YIVY+GS P     TP S H ++L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 67  APESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQSV 126
               L+ SYK+SFNGFA RLTE E  ++A  E VVSVFP+ K  L TT SW+FMG  + +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 127 --PRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGAR 186
              R   +ESD ++GV+D+GI+PES SF D+ FGPPP KW G C    +F CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 187 VYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYK 246
            Y ++S      ++ RD +GHGTHTAS  AG  V  ++ YGLG GTARGGVP+ RIAVYK
Sbjct: 187 DYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYK 246

Query: 247 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSN 306
           +C ++GC    +++AFDDAIADGVD+IS+S+       +  DPIAIGAFHAM  G+LT N
Sbjct: 247 VCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVN 306

Query: 307 SAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYP 366
           +AGN GP   T ++ +PW  SVAAS T+R  + +V LG+G I   G ++NT+D+ G  YP
Sbjct: 307 AAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKIL-IGRSVNTYDMNGTNYP 366

Query: 367 LIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMR 426
           L+Y   A       +   +RLC    +D  LV+GKI++CDS  G  +     A VG +++
Sbjct: 367 LVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGA-VGSIVK 426

Query: 427 DLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSR 486
           +    D A    FP S+L       +  Y++ST +P AT+LKS  + +  AP+V SFSSR
Sbjct: 427 NPE-PDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSR 486

Query: 487 GPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIA 546
           GP+ I  DILKPD++APGVEILAA+SP +  +  + D+R   Y+++SGTSM+CPH   +A
Sbjct: 487 GPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVA 546

Query: 547 AYVKTFHPTWSPAALKSALMTTAFTMNAKLN--PQAEFAYGSGHVNPLKAVNPGLVYDAN 606
           AYVKTFHP WSP+ ++SA+MTTA+ MNA  +     EFAYGSGHV+P+ A+NPGLVY+  
Sbjct: 547 AYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELT 606

Query: 607 EGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVW--DLNYPSFALSTAPSRPINQFF 666
           + D++ FLCG  Y++  +R I+GD+S CT   I +    +LNYP+ +   + ++P N  F
Sbjct: 607 KADHINFLCGLNYTSDHLRIISGDNSTCTK-EISKTLPRNLNYPTMSAKVSGTKPFNITF 666

Query: 667 RRTLTNVESGASTYSA-VVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTV--SQAI 726
           +RT+TNV    STY+A VV  P   L+I V+P VLS   + + +SF +TV      ++  
Sbjct: 667 QRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 726

Query: 727 VSASLVWSDGVHQVRSPITIY 736
           VSA+L+WSDG H VRSPI +Y
Sbjct: 727 VSANLIWSDGTHNVRSPIIVY 738

BLAST of MS017252 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 2.5e-181
Identity = 353/725 (48.69%), Postives = 460/725 (63.45%), Query Frame = 0

Query: 20  VSGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEEVIGSTFAPESLLYSYKRSFNG 79
           VS   +D   ++ Y+VYMGS P     TP S+H  +L+EV G +     L+ SYKRSFNG
Sbjct: 21  VSAIIYDPQDKQVYVVYMGSLPSQPNYTPMSNHINILQEVTGESSIEGRLVRSYKRSFNG 80

Query: 80  FAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQ--SVPRAHQVESDVVVG 139
           F+  LTE E   VA  EGVVSVF +    L TT SWDFMG  +  +  R   VESD ++G
Sbjct: 81  FSALLTESEREGVAEMEGVVSVFRSKNYKLQTTASWDFMGMKEGKNTKRNFAVESDTIIG 140

Query: 140 VLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARVYRSESLPPGDIRSP 199
            +D+GIWPES SF D+ FGPPP KW G C+   +F CN K+IGAR Y SE          
Sbjct: 141 FIDSGIWPESESFSDKGFGPPPKKWKGVCKGGKNFTCNNKLIGARDYTSE--------GT 200

Query: 200 RDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYKICWSDGCSDADILAA 259
           RD  GHGTHT ST AG  V   S +G+G GTARGGVP++R+A YK+C   GCSD ++L+A
Sbjct: 201 RDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPASRVAAYKVCTITGCSDDNVLSA 260

Query: 260 FDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSNSAGNEGPNYFTASNI 319
           FDDAIADGVD+IS+S+GG  P  Y  D IAIGAFHAM  GILT +SAGN GPN  T  ++
Sbjct: 261 FDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAFHAMAKGILTVHSAGNAGPNPTTVVSV 320

Query: 320 SPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYPLIYAGDAPNIAGGFN 379
           +PW L+VAA+TT+R+ LT+V LGNG     G ++N FDL GK+YPL Y GD  N      
Sbjct: 321 APWMLTVAATTTNRRFLTKVVLGNGKTL-VGKSVNAFDLKGKKYPLEY-GDYLN------ 380

Query: 380 GSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMRDLRLKDYASSYPFPA 439
                        ++LV+GKILV   L G+    SF  T          KDYAS    P 
Sbjct: 381 -------------ESLVKGKILVSRYLSGSEVAVSFITTDN--------KDYASISSRPL 440

Query: 440 SYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSRGPNFITRDILKPDLS 499
           S L     D +  YI+ST  P  ++LK+ A+ +  +P V SFSSRGPN I  DILKPD+S
Sbjct: 441 SVLSQDDFDSLVSYINSTRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIAVDILKPDIS 500

Query: 500 APGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIAAYVKTFHPTWSPAAL 559
           APGVEILAA+SPL+  S  + D R   Y+++SGTSM+CPH T +AAY+KTFHP WSP+ +
Sbjct: 501 APGVEILAAYSPLSLPSEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTFHPDWSPSVI 560

Query: 560 KSALMTTAFTMNAKLN--PQAEFAYGSGHVNPLKAVNPGLVYDANEGDYVKFLCGQGYST 619
           +SA+MTTA+ MNA        EFAYG+GHV+P+ A+NPGLVY+ N+ D++ FLCG  Y++
Sbjct: 561 QSAIMTTAWQMNATGTGAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGMNYTS 620

Query: 620 AMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSR-PINQFFRRTLTNVESGASTY-S 679
             ++ I+GD+  C+   + R  +LNYPS +   + S       F+RT+TN+ +  STY S
Sbjct: 621 KTLKLISGDAVICSGKTLQR--NLNYPSMSAKLSESNSSFTVTFKRTVTNLGTANSTYKS 680

Query: 680 AVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIV--SASLVWSDGVHQVRS 736
            +V      LN+ V+PSVLS   + + +SF +TV G+     +  SA+L+WSDG H VRS
Sbjct: 681 KIVLNHGSKLNVKVSPSVLSMKSLKEKQSFTVTVSGSNIDPKLPSSANLIWSDGTHNVRS 706

BLAST of MS017252 vs. ExPASy TrEMBL
Match: A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)

HSP 1 Score: 1434.5 bits (3712), Expect = 0.0e+00
Identity = 726/741 (97.98%), Postives = 728/741 (98.25%), Query Frame = 0

Query: 1   TMSSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIG 60
           TMSSLIFKFIFFSLFYRLLVSGSH DNDGRKTYIVYMGSKPEDTTSTP HHRAMLEEVIG
Sbjct: 6   TMSSLIFKFIFFSLFYRLLVSGSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAMLEEVIG 65

Query: 61  STF---APESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM 120
           STF   APESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM
Sbjct: 66  STFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM 125

Query: 121 GFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKI 180
           GFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKI
Sbjct: 126 GFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKI 185

Query: 181 IGARVYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRI 240
           IGARVYRSESLPP DIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPS RI
Sbjct: 186 IGARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSARI 245

Query: 241 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGI 300
           AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEP SYFSDPIAIGAFHAMKHGI
Sbjct: 246 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMKHGI 305

Query: 301 LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLG 360
           LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIY QGVAINTFDLLG
Sbjct: 306 LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIY-QGVAINTFDLLG 365

Query: 361 KQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVG 420
           KQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVG
Sbjct: 366 KQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVG 425

Query: 421 IVMRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVS 480
           IVM+DLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVS
Sbjct: 426 IVMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVS 485

Query: 481 FSSRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHA 540
           FSSRGP FITRDILK DLSAPGVEILAAWSPLAPV GVKGDSRSELYNIISGTSMSCPHA
Sbjct: 486 FSSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHA 545

Query: 541 TAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYD 600
           TAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYD
Sbjct: 546 TAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYD 605

Query: 601 ANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF 660
           ANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF
Sbjct: 606 ANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF 665

Query: 661 RRTLTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSA 720
           RRTLTNVESGASTYSAVVFGPPDGL+ITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSA
Sbjct: 666 RRTLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSA 725

Query: 721 SLVWSDGVHQVRSPITIYFLK 739
           SL WSDGVHQVRSPITIYFLK
Sbjct: 726 SLEWSDGVHQVRSPITIYFLK 745

BLAST of MS017252 vs. ExPASy TrEMBL
Match: A0A1S3CF95 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 566/738 (76.69%), Postives = 622/738 (84.28%), Query Frame = 0

Query: 5   LIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGSTFA 64
           LI  F+F +LF    V GSH DNDGRKTYIVYMGS  EDT+STP HHRAMLE+VIGS FA
Sbjct: 12  LILNFVFLNLFNCQPVFGSHLDNDGRKTYIVYMGSTLEDTSSTPLHHRAMLEQVIGSNFA 71

Query: 65  PESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQSVP 124
           P+ LLYSYKRSFNGFAVRLTEEEA K+A KEGVVSVFPNGKKH+HTTRSWDFMGF+QSVP
Sbjct: 72  PKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVP 131

Query: 125 RAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARVYR 184
           R +QVES++VVGVLDTGIWPESPSF D    PPPA W G CQ + DF+CN+KIIGAR YR
Sbjct: 132 RVNQVESNIVVGVLDTGIWPESPSFNDADLDPPPAGWKGQCQTSPDFQCNRKIIGARTYR 191

Query: 185 SESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYKICW 244
           SE+LPPG+ +SPRDS GHGTHTASTVAGGLV QASLYGLG GTARGGVPS RIAVYKICW
Sbjct: 192 SENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYKICW 251

Query: 245 SDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSNSAG 304
           SDGC DADILAAFDDAIADGVDIISLSVGGS  KSYF+D IAIGAFHAMKHGILTSNSAG
Sbjct: 252 SDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMKHGILTSNSAG 311

Query: 305 NEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYPLIY 364
           NEGP YFT SN+SPWSLSVAAST DRK +++VQL NG  Y QG AI+TFDL+GKQYPLIY
Sbjct: 312 NEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAY-QGPAIHTFDLMGKQYPLIY 371

Query: 365 AGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASF--NATVGIVMRD 424
            GDAPN +GGFN SISR C ENSVD++LV+GKILVCDS+L AS + SF  N  VGI+M+ 
Sbjct: 372 GGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKNGAVGIIMQG 431

Query: 425 LRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSRG 484
            R KDYASSYP PASYL  A         + T   TA+I KS A+ D+SAP VVSFSSRG
Sbjct: 432 SRFKDYASSYPLPASYLHNA---------NITLSSTASIFKSNAILDSSAPSVVSFSSRG 491

Query: 485 PNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIAA 544
           PN  T DILKPDL+APGVEILAAWSP+A VSGV GDSRS LYNIISGTSMSCPHATAIA 
Sbjct: 492 PNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAV 551

Query: 545 YVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANEGD 604
           YVKTF+PTWSPAA+KSALMTTAF+MNAK+NPQAEFAYG+GH+NPLKAVNPGLVY+A E D
Sbjct: 552 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETD 611

Query: 605 YVKFLCGQ-GYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSR-PINQFFRRT 664
           Y+ FLCGQ GY+T MVRRITGD++ACTP N GRVWDLNYPSFA ST PS+  INQFF RT
Sbjct: 612 YINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFART 671

Query: 665 LTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLV 724
           LTNVES  S YSA VF PP  L ITV+P  L FNG+GDTKSF LTVQGTV+Q I+S SLV
Sbjct: 672 LTNVESKTSLYSAKVFAPP-SLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLV 731

Query: 725 WSDGVHQVRSPITIYFLK 739
           W+DGVHQVRSPIT+Y  K
Sbjct: 732 WTDGVHQVRSPITVYVAK 738

BLAST of MS017252 vs. ExPASy TrEMBL
Match: A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 553/735 (75.24%), Postives = 622/735 (84.63%), Query Frame = 0

Query: 3   SSLIFKFIFFSLFYRLLVSGSHFDN-DGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGS 62
           SSLIFK +F SL   LL S    +N DGRK YIVY+G+KPEDT STPSHH  MLEEV+GS
Sbjct: 3   SSLIFKLVFLSLLCGLLASSLDSNNDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVVGS 62

Query: 63  TFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQ 122
           TFAPE+LL+SYKRSFNGF V+LTEEEA K+AAKEGVVSVF NGKKHLHTTRSWDFMGF++
Sbjct: 63  TFAPEALLHSYKRSFNGFVVKLTEEEAQKIAAKEGVVSVFLNGKKHLHTTRSWDFMGFTK 122

Query: 123 SVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGAR 182
           +V R  QVES++VVGVLD+GIWPESPSF D  +GPPP KW G CQ + +F CNKKIIGAR
Sbjct: 123 NVARVKQVESNIVVGVLDSGIWPESPSFSDLGYGPPPVKWKGTCQTSANFHCNKKIIGAR 182

Query: 183 VYRSES-LPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVY 242
            YRS +  PP DIRSPRDS+GHGTHTASTVAGGLV QASLYGL +GTARGGVPS RIAVY
Sbjct: 183 AYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 242

Query: 243 KICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTS 302
           KICWSDGC DADILAAFDDAIADGVDIISLSVGGSEPK YF+D IAIGAFH+MKHGILTS
Sbjct: 243 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 302

Query: 303 NSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQY 362
           NSAGN+GP+YFT  N SPWSLSVAAS+ DRK +T+VQLGN N+Y QG  INTFDLLGKQY
Sbjct: 303 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLY-QGYTINTFDLLGKQY 362

Query: 363 PLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVM 422
           PLIY GDAPNI GGF  S SR C +N+VD+NLV+GKILVCD++L  S  ASF   VG+VM
Sbjct: 363 PLIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASFXGAVGVVM 422

Query: 423 RDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSS 482
           +D  +KD A SYP PASYL TAA   I+ Y++S    TATILKS AV D SAP+VVSFSS
Sbjct: 423 QDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSFSS 482

Query: 483 RGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAI 542
           RGPN  T DILKPDL+APGVEILAAWSP+AP+SG+  DSR  LYNIISGTSMSCPHATA+
Sbjct: 483 RGPNPETFDILKPDLTAPGVEILAAWSPIAPISGIT-DSRKSLYNIISGTSMSCPHATAV 542

Query: 543 AAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANE 602
           A YVKTFHPTWSPAA+KSALMTTA  +N KLNPQAEFAYG+GHVNPLKAVNPGLVYDA+E
Sbjct: 543 AVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDAHE 602

Query: 603 GDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRRT 662
            DYVKFLCGQGY+TAMV+R+T D+SACTP N  RVWDLNYPSFALS+ PS  INQFF RT
Sbjct: 603 SDYVKFLCGQGYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSESINQFFTRT 662

Query: 663 LTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLV 722
           LTNV S ASTY A V+G P GL I+V+P VLSF+ +G+ KSF LT++GT+S +IVSASLV
Sbjct: 663 LTNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTLSHSIVSASLV 722

Query: 723 WSDGVHQVRSPITIY 736
           W+DG+HQVRSPIT+Y
Sbjct: 723 WNDGLHQVRSPITVY 735

BLAST of MS017252 vs. ExPASy TrEMBL
Match: A0A6J1K719 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 543/736 (73.78%), Postives = 620/736 (84.24%), Query Frame = 0

Query: 3   SSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGST 62
           SS+IFK +F   F  LL S    DNDGRK YIVY+G+KP+D  STPSHH  +LEEV+GST
Sbjct: 15  SSVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGST 74

Query: 63  FAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQS 122
           F+P++LL+SYKRSFNGFAVRLTEEEA K+ AK+GVVSVFPNGKKHLHTTRSWDFMGF+ +
Sbjct: 75  FSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTN 134

Query: 123 VPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARV 182
           VPR +QVESD+VVGVLD+GIWPESPSF D  +GP PAKW GACQA+++FRCNKKIIGAR 
Sbjct: 135 VPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARA 194

Query: 183 YRSE-SLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYK 242
           YRS+ + PP D  SPRDS+GHGTHTASTVAGGLV QASLYGL +GTARGGVPS RIAVYK
Sbjct: 195 YRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYK 254

Query: 243 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSN 302
           ICWSDGC DADILAAFDDAIADGVDIISLSVGGSEPK YF+D IAIGAFH+MKHGILTSN
Sbjct: 255 ICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSN 314

Query: 303 SAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYP 362
           SAGN+GP+YFT  N SPWSLSVAAS+ DRK+++ VQLGN NI+ QG  INTFDL GK YP
Sbjct: 315 SAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIF-QGYTINTFDLQGKHYP 374

Query: 363 LIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMR 422
           LIYAG APNI+GGF+GS SR C +NSVD+NLV+GKILVCDS+L  S  AS ++ VG+VM 
Sbjct: 375 LIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMN 434

Query: 423 DLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSR 482
           +  +KD A SYP P+SYL  AA + ++ YI S   PTATI KS  V D +AP+VVSFSSR
Sbjct: 435 NAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSR 494

Query: 483 GPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIA 542
           GPN  T DILKPDL+APGVEILAAW+P+APVS    DSR+ LYNIISGTSMSCPHATA A
Sbjct: 495 GPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAA 554

Query: 543 AYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANEG 602
            YVKTFHPTWSPAA+KSALMTTA  +N +LNPQAEFAYG+GH++PLKA+NPGL+YDANE 
Sbjct: 555 VYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANET 614

Query: 603 DYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRRTL 662
           DYV FLCGQGYSTAMVRR+TGD S CT  N GRVWDLNYPSFALST PS  INQFF RTL
Sbjct: 615 DYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTL 674

Query: 663 TNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLVW 722
           TNV S ASTY++ + G P+GL ITV+P VLSFNG+G  +SF LT+ GT+SQ+IVSAS+VW
Sbjct: 675 TNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVVW 734

Query: 723 SDGVHQVRSPITIYFL 738
           SDG H VRSPITIY +
Sbjct: 735 SDGSHNVRSPITIYIV 749

BLAST of MS017252 vs. ExPASy TrEMBL
Match: A0A6J1FVT5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447322 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 538/736 (73.10%), Postives = 612/736 (83.15%), Query Frame = 0

Query: 3   SSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTPSHHRAMLEEVIGST 62
           S +IFK +F   F  LL S     NDGRK YIVY+G+KP+D  STPSHH  +LEEV+GST
Sbjct: 17  SYVIFKLVFLGFFGCLLASSLDSVNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGST 76

Query: 63  FAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQS 122
           FAP++LL+SYKRSFNGF VRLTE EA K+ AK+GVVSVFPNG+KHLHTTRSWDFMGF+ +
Sbjct: 77  FAPDALLHSYKRSFNGFVVRLTEGEAQKIRAKDGVVSVFPNGRKHLHTTRSWDFMGFTTN 136

Query: 123 VPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARV 182
           VPR +QVESD+VVGVLD+GIWPESPSF D  +GPPPAKW GACQA+ +FRCNKKIIGAR 
Sbjct: 137 VPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQASTNFRCNKKIIGARA 196

Query: 183 YRSE-SLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYK 242
           YRS+ + PP D  SPRDS+GHGTHTASTVAGGLV QASLYGL +GTARGGVPS RIAVYK
Sbjct: 197 YRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYK 256

Query: 243 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSN 302
           ICWSDGC DADILAAFDDAIADGVDIISLSVGGSEP+ YF+D IAIGAFH+MKHGILTSN
Sbjct: 257 ICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPRYYFNDSIAIGAFHSMKHGILTSN 316

Query: 303 SAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYP 362
           SAGN+GP+YFT  N SPWSLSVAAS+ DRK++++VQLGN NI+ QG  INTFDL GKQYP
Sbjct: 317 SAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIF-QGYTINTFDLQGKQYP 376

Query: 363 LIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMR 422
           LIYAG APNI+GGF  S SR C +NSVD+NLV+GKI+VCDS+L  S   S ++ VG+VM 
Sbjct: 377 LIYAGHAPNISGGFTSSSSRYCSQNSVDRNLVKGKIVVCDSILSPSTFVSLSSAVGVVMN 436

Query: 423 DLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSR 482
           D  LKD A SYP P+SYL  AA + ++ Y+ S   PTATI KS  V D SAP+VVSFSSR
Sbjct: 437 DAGLKDNARSYPLPSSYLTRAAGNHVKNYMGSNNFPTATIHKSNEVNDTSAPIVVSFSSR 496

Query: 483 GPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIA 542
           GPN  T DILKPDL+APGVEILAAW+P+APVS    DSR+ LYNIISGTSMSCPHATA A
Sbjct: 497 GPNPETNDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAA 556

Query: 543 AYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDANEG 602
            YVKTFHPTWSPAA+KSALMTTA  +N +LNPQAEFAYG+GH++PLKA NPGLVYDANE 
Sbjct: 557 VYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKATNPGLVYDANET 616

Query: 603 DYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRRTL 662
           DYV FLCGQGYSTAMV+R+TGD S CT  N GRVWDLNYPSFALST PS  INQFF RTL
Sbjct: 617 DYVNFLCGQGYSTAMVQRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTL 676

Query: 663 TNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASLVW 722
           TNV S ASTY++ + G P+GL ITV+P  LSFN +GD KSF LT+ GT+SQ+IVSAS+ W
Sbjct: 677 TNVGSEASTYTSKILGAPEGLTITVDPPALSFNSIGDEKSFTLTIDGTISQSIVSASVEW 736

Query: 723 SDGVHQVRSPITIYFL 738
           SDG H VRSPITIY +
Sbjct: 737 SDGSHNVRSPITIYIV 751

BLAST of MS017252 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 648.3 bits (1671), Expect = 7.6e-186
Identity = 339/706 (48.02%), Postives = 460/706 (65.16%), Query Frame = 0

Query: 37  MGSKPEDTTSTPSHHRAMLEEVIGSTFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEG 96
           MG+ PE   S PSHH ++L++++G+  A   L+ SYKRSFNGFA  L++ E+ K+   + 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 97  VVSVFPNGKKHLHTTRSWDFMGFSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGP 156
           VVSVFP+    L TTRSWDF+GF +   R    ESDV+VGV+D+GIWPES SF DE FGP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 157 PPAKWMGACQAADDFRCNKKIIGARVYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVE 216
           PP KW G+C+    F CN K+IGAR Y   +       S RD  GHGTHTAST AG  V+
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFA------DSARDEEGHGTHTASTAAGNAVQ 180

Query: 217 QASLYGLGIGTARGGVPSTRIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSE 276
            AS YGL  GTARGGVPS RIA YK+C+ + C+D DILAAFDDAIADGVD+IS+S+    
Sbjct: 181 AASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADY 240

Query: 277 PKSYFSDPIAIGAFHAMKHGILTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQV 336
             +  +  +AIG+FHAM  GI+T+ SAGN GP+  + +N+SPW ++VAAS TDR+ + +V
Sbjct: 241 VSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRV 300

Query: 337 QLGNGNIYQQGVAINTFDLLGKQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGK 396
            LGNG     G+++NTF+L G ++P++Y     N++   + + +  C    VD  LV+GK
Sbjct: 301 VLGNGKAL-TGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 397 ILVCDSLLGASKLASFNATVGIVMRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTD 456
           I++CD  LG  + A     +G+++++  L D A   PFPAS L       I+ YI S   
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 457 PTATILKSIAVQDASAPVVVSFSSRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVK 516
           P A IL++  + D  AP V SFSSRGP+F+ +++LKPD+SAPG+EILAA+SP+A  S   
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 517 G--DSRSELYNIISGTSMSCPHATAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQ 576
              D RS  Y+++SGTSM+CPH   +AAYVK+FHP WSP+A+KSA+MTTA  MN K NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 577 AEFAYGSGHVNPLKAVNPGLVYDANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGR 636
            EFAYGSG +NP KA +PGLVY+    DY+K LC +G+ +  +   +G +  C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 637 VWDLNYPSFALSTAPSRPINQFFRRTLTNVESGASTYSAVVFGPPDGLNITVNPSVLSFN 696
           V DLNYP+     +   P N  F+RT+TNV    STY A V      L I++ P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 697 GVGDTKSFNLTVQGTVSQ--AIVSASLVWSDGVHQVRSPITIYFLK 739
            + + KSF +T+ G   +  + VS+S+VWSDG H VRSPI  Y ++
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSIQ 692

BLAST of MS017252 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 640.2 bits (1650), Expect = 2.1e-183
Identity = 355/743 (47.78%), Postives = 472/743 (63.53%), Query Frame = 0

Query: 2   MSSLIFKFIFFSLFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEEVIG 61
           M++L       S    L +S      D ++ YIVYMGS       TP S H  +L+EV G
Sbjct: 1   MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60

Query: 62  STFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFS 121
            +     L+ SYKRSFNGFA RLTE E  +VA   GVVSVFPN K  L TT SWDFMG  
Sbjct: 61  ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120

Query: 122 QSV--PRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKII 181
           + +   R   VESD ++GV+D+GI PES SF D+ FGPPP KW G C    +F CN K+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180

Query: 182 GARVYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIA 241
           GAR Y SE          RD +GHGTHTAST AG  V  AS +G+G GT RGGVP++R+A
Sbjct: 181 GARDYTSE--------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVA 240

Query: 242 VYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGIL 301
            YK+C   GCS   +L+AFDDAIADGVD+I++S+G      + +DPIAIGAFHAM  G+L
Sbjct: 241 AYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVL 300

Query: 302 TSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGK 361
           T NSAGN GP   + S ++PW L+VAASTT+R  +T+V LGNG     G ++N +++ GK
Sbjct: 301 TVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTL-VGKSVNAYEMKGK 360

Query: 362 QYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGI 421
            YPL+Y   A + A   +   + LC  + VDK+ V+GKILVC    G  K+      VG+
Sbjct: 361 DYPLVYGKSAASSA--CDAESAGLCELSCVDKSRVKGKILVCGG-PGGLKIVESVGAVGL 420

Query: 422 VMRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSF 481
           + R  +  D A  +P PA+ L T   + +  Y+ ST  P A +LK+ A+ + ++PV+ SF
Sbjct: 421 IYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASF 480

Query: 482 SSRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHAT 541
           SSRGPN I  DILKPD++APGVEILAA+SP    S  + D+R   Y+++SGTSMSCPH  
Sbjct: 481 SSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHVA 540

Query: 542 AIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLN--PQAEFAYGSGHVNPLKAVNPGLVY 601
            +AAYVKTF+P WSP+ ++SA+MTTA+ +NA        EFAYGSGHV+P+ A NPGLVY
Sbjct: 541 GVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVY 600

Query: 602 DANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPS-RPINQ 661
           + ++ D++ FLCG  Y++ +++ I+G++  C+        +LNYPS +   + S      
Sbjct: 601 ELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTV 660

Query: 662 FFRRTLTNVESGASTY-SAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGT--VSQ 721
            F RTLTNV +  STY S VV G    L++ + PSVLSF  V + +SF +TV G+   S+
Sbjct: 661 TFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSE 720

Query: 722 AIVSASLVWSDGVHQVRSPITIY 736
              SA+L+WSDG H VRSPI +Y
Sbjct: 721 VPSSANLIWSDGTHNVRSPIVVY 728

BLAST of MS017252 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 637.1 bits (1642), Expect = 1.7e-182
Identity = 349/741 (47.10%), Postives = 480/741 (64.78%), Query Frame = 0

Query: 7   FKFIFFSLFYRLL--VSGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEEVIGSTF 66
           F F+F SL    L  VS    D+  ++ YIVY+GS P     TP S H ++L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 67  APESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQSV 126
               L+ SYK+SFNGFA RLTE E  ++A  E VVSVFP+ K  L TT SW+FMG  + +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 127 --PRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGAR 186
              R   +ESD ++GV+D+GI+PES SF D+ FGPPP KW G C    +F CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 187 VYRSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYK 246
            Y ++S      ++ RD +GHGTHTAS  AG  V  ++ YGLG GTARGGVP+ RIAVYK
Sbjct: 187 DYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYK 246

Query: 247 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSN 306
           +C ++GC    +++AFDDAIADGVD+IS+S+       +  DPIAIGAFHAM  G+LT N
Sbjct: 247 VCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVN 306

Query: 307 SAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYP 366
           +AGN GP   T ++ +PW  SVAAS T+R  + +V LG+G I   G ++NT+D+ G  YP
Sbjct: 307 AAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKIL-IGRSVNTYDMNGTNYP 366

Query: 367 LIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMR 426
           L+Y   A       +   +RLC    +D  LV+GKI++CDS  G  +     A VG +++
Sbjct: 367 LVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGA-VGSIVK 426

Query: 427 DLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSR 486
           +    D A    FP S+L       +  Y++ST +P AT+LKS  + +  AP+V SFSSR
Sbjct: 427 NPE-PDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSR 486

Query: 487 GPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIA 546
           GP+ I  DILKPD++APGVEILAA+SP +  +  + D+R   Y+++SGTSM+CPH   +A
Sbjct: 487 GPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVA 546

Query: 547 AYVKTFHPTWSPAALKSALMTTAFTMNAKLN--PQAEFAYGSGHVNPLKAVNPGLVYDAN 606
           AYVKTFHP WSP+ ++SA+MTTA+ MNA  +     EFAYGSGHV+P+ A+NPGLVY+  
Sbjct: 547 AYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELT 606

Query: 607 EGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVW--DLNYPSFALSTAPSRPINQFF 666
           + D++ FLCG  Y++  +R I+GD+S CT   I +    +LNYP+ +   + ++P N  F
Sbjct: 607 KADHINFLCGLNYTSDHLRIISGDNSTCTK-EISKTLPRNLNYPTMSAKVSGTKPFNITF 666

Query: 667 RRTLTNVESGASTYSA-VVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGTV--SQAI 726
           +RT+TNV    STY+A VV  P   L+I V+P VLS   + + +SF +TV      ++  
Sbjct: 667 QRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 726

Query: 727 VSASLVWSDGVHQVRSPITIY 736
           VSA+L+WSDG H VRSPI +Y
Sbjct: 727 VSANLIWSDGTHNVRSPIIVY 738

BLAST of MS017252 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 636.7 bits (1641), Expect = 2.3e-182
Identity = 352/733 (48.02%), Postives = 469/733 (63.98%), Query Frame = 0

Query: 14  LFYRLLVSGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEEVIGSTFAPESLLYSY 73
           L   LL S S   ++  + YIVYMGS        P S H ++L++V G +     L+ SY
Sbjct: 14  LLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSY 73

Query: 74  KRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMGFSQ--SVPRAHQVE 133
           KRSFNGFA RLTE E   +A  EGVVSVFPN    LHTT SWDFMG  +  +  R   +E
Sbjct: 74  KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 133

Query: 134 SDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKIIGARVYRSESLPP 193
           SD ++GV+DTGIWPES SF D+ FGPPP KW G C    +F CN K+IGAR Y SE    
Sbjct: 134 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE---- 193

Query: 194 GDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSTRIAVYKICWSDGCSD 253
                 RD++GHGTHTAST AG  V+  S +G+G GT RGGVP++RIA YK+C   GCS 
Sbjct: 194 ----GTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSS 253

Query: 254 ADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFHAMKHGILTSNSAGNEGPNY 313
             +L++FDDAIADGVD+I++S+G   P  +  DPIAIGAFHAM  GILT +SAGN GP  
Sbjct: 254 EALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKP 313

Query: 314 FTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAINTFDLLGKQYPLIYAGDAPN 373
            T S+++PW  +VAASTT+R  +T+V LGNG     G ++N FD+ GK+YPL+Y   A +
Sbjct: 314 TTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTL-AGRSVNAFDMKGKKYPLVYGKSAAS 373

Query: 374 IAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIVMRDLRLKDYAS 433
            A   +   + LC    ++K+ V+GKILVC    G  K+A     + I+ +  R  D A 
Sbjct: 374 SA--CDAKTAALCAPACLNKSRVKGKILVCGGPSG-YKIAKSVGAIAIIDKSPR-PDVAF 433

Query: 434 SYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFSSRGPNFITRDI 493
           ++  PAS L+      +  YI S   P A +LK+  + + ++PV+ SFSSRGPN I  DI
Sbjct: 434 THHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDI 493

Query: 494 LKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTSMSCPHATAIAAYVKTFHPT 553
           LKPD++APGVEILAA+SP    S  + D+R   Y++ SGTSM+CPH   +AAYVKTF+P 
Sbjct: 494 LKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPR 553

Query: 554 WSPAALKSALMTTAFTMNAKLN--PQAEFAYGSGHVNPLKAVNPGLVYDANEGDYVKFLC 613
           WSP+ ++SA+MTTA+ + AK       EFAYG+GHV+P+ A+NPGLVY+ ++ D++ FLC
Sbjct: 554 WSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLC 613

Query: 614 GQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSF-ALSTAPSRPINQFFRRTLTNVESG 673
           G  Y++  ++ I+GD+  C+  N     +LNYPS  A  +      +  F RTLTNV + 
Sbjct: 614 GMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTP 673

Query: 674 ASTY-SAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQGT--VSQAIVSASLVWSDG 733
            STY S VV G    L+I V PSVL F  V + +SF++TV G+   S+   SA+L+WSDG
Sbjct: 674 NSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDG 731

Query: 734 VHQVRSPITIYFL 738
            H VRSPI +Y +
Sbjct: 734 THNVRSPIVVYIM 731

BLAST of MS017252 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 635.6 bits (1638), Expect = 5.1e-182
Identity = 355/750 (47.33%), Postives = 474/750 (63.20%), Query Frame = 0

Query: 2   MSSLIFKFIFFSLFYRLLV---SGSHFDNDGRKTYIVYMGSKPEDTTSTP-SHHRAMLEE 61
           M+     +   S  + LLV   + +  D+  ++ YIVYMG+ P      P SHH ++L++
Sbjct: 1   MTKPAVSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQD 60

Query: 62  VIGSTFAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFM 121
           V G +   + L+ +YKRSFNGFA RLT+ E   +A+ + VVSVFPN K  L TT SW+FM
Sbjct: 61  VTGESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFM 120

Query: 122 GFSQS--VPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNK 181
           G  +S    R   +ESD ++GV+D+GI+PES SF  + FGPPP KW G C+   +F  N 
Sbjct: 121 GLKESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNN 180

Query: 182 KIIGARVY--RSESLPPGDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVP 241
           K+IGAR Y  + E  P     S RD  GHG+HTAST AG  V+  S YGLG GTARGGVP
Sbjct: 181 KLIGARYYTPKLEGFP----ESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVP 240

Query: 242 STRIAVYKIC--WSDGCSDADILAAFDDAIADGVDIISLSVGGSEPKSYFSDPIAIGAFH 301
           + RIAVYK+C    DGC+   ILAAFDDAIAD VDII++S+GG     +  DPIAIGAFH
Sbjct: 241 AARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFH 300

Query: 302 AMKHGILTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQQGVAIN 361
           AM  GIL  NSAGN GP   T ++I+PW  +VAAS T+R  +T+V LGNG     G ++N
Sbjct: 301 AMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV--GRSVN 360

Query: 362 TFDLLGKQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLAS 421
           +FDL GK+YPL+Y   A +  G  +      C    +D   V+GKI++CDS     +  +
Sbjct: 361 SFDLNGKKYPLVYGKSASSSCGAASAG---FCSPGCLDSKRVKGKIVLCDSPQNPDEAQA 420

Query: 422 FNATVGIVMRDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDAS 481
             A   IV       D AS + FP S L     + +  Y++ST +P A +LKS  + +  
Sbjct: 421 MGAIASIVRS--HRTDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQR 480

Query: 482 APVVVSFSSRGPNFITRDILKPDLSAPGVEILAAWSPLAPVSGVKGDSRSELYNIISGTS 541
           APVV S+ SRGPN I  DILKPD++APG EI+AA+SP AP S    D+R   Y++ +GTS
Sbjct: 481 APVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTS 540

Query: 542 MSCPHATAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNP---QAEFAYGSGHVNPLK 601
           MSCPH   +AAY+K+FHP WSP+ ++SA+MTTA+ MNA  +P    AEFAYG+GHV+P+ 
Sbjct: 541 MSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPIT 600

Query: 602 AVNPGLVYDANEGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRV-WDLNYPSFALST 661
           A++PGLVY+AN+ D++ FLCG  Y+   +R I+GDSS+CT      +  +LNYPS     
Sbjct: 601 AIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQV 660

Query: 662 APSRPINQFFRRTLTNVESGASTYSAVVFGPPDGLNITVNPSVLSFNGVGDTKSFNLTVQ 721
           + ++P    FRRT+TNV    +TY A V G    L + V P+VLS   + + KSF +T  
Sbjct: 661 SAAKPFKVIFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTAS 720

Query: 722 GTVSQA--IVSASLVWSDGVHQVRSPITIY 736
           G   +A  +VSA L+WSDGVH VRSPI +Y
Sbjct: 721 GAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735