MS017214 (gene) Bitter gourd (TR) v1

BLAST of MS017214 vs. NCBI nr
Match: XP_022156924.1 (uncharacterized protein At2g39920 [Momordica charantia] >XP_022156925.1 uncharacterized protein At2g39920 [Momordica charantia] >XP_022156926.1 uncharacterized protein At2g39920 [Momordica charantia])

HSP 1 Score: 553.5 bits (1425), Expect = 1.0e-153
Identity = 275/280 (98.21%), Postives = 276/280 (98.57%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK
Sbjct: 26  SDMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
           PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA
Sbjct: 86  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
           VQV NGRDIVLMDIDDVLFENPGYDNLLIPC CVDEAKILKQKFLLQLYKKLRSCGWPLI
Sbjct: 146 VQVNNGRDIVLMDIDDVLFENPGYDNLLIPC-CVDEAKILKQKFLLQLYKKLRSCGWPLI 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATIERLT AG KGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG
Sbjct: 206 LISRKSEVHRNATIERLTSAGCKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQI 281
           VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQI
Sbjct: 266 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQI 304

BLAST of MS017214 vs. NCBI nr
Match: XP_022978228.1 (uncharacterized protein At2g39920 isoform X1 [Cucurbita maxima] >XP_022978236.1 uncharacterized protein At2g39920 isoform X1 [Cucurbita maxima] >XP_022978244.1 uncharacterized protein At2g39920 isoform X1 [Cucurbita maxima])

HSP 1 Score: 439.1 bits (1128), Expect = 2.8e-119
Identity = 218/276 (78.99%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVHNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD+DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDLDDLLFANHDYDDLLISSSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGYKGWL LIMRMDD++QI H EYF++QQAAL  EGFRIVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYKGWLHLIMRMDDKMQISHREYFSQQQAALRKEGFRIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+Q + K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQKLSK 301

BLAST of MS017214 vs. NCBI nr
Match: XP_038891286.1 (uncharacterized protein At2g39920 isoform X1 [Benincasa hispida] >XP_038891287.1 uncharacterized protein At2g39920 isoform X1 [Benincasa hispida] >XP_038891288.1 uncharacterized protein At2g39920 isoform X1 [Benincasa hispida])

HSP 1 Score: 438.3 bits (1126), Expect = 4.8e-119
Identity = 215/279 (77.06%), Postives = 243/279 (87.10%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGSRYI+ESGFYM PLTATIFIGALVTVGVLLITLLVSLTVMLQYCQN+S G+VEIQK
Sbjct: 26  SDMGSRYIIESGFYMAPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGIVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
             V YDYCKALSVHLELNSLETDGIPSFCK+ +IQYIK+GQYERDL S LQVVD YFC A
Sbjct: 86  SSVDYDYCKALSVHLELNSLETDGIPSFCKDVSIQYIKSGQYERDLYSILQVVDKYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N +DIVLMDIDD+LF N  Y++LLI  SCVD+AK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVGNSQDIVLMDIDDLLFTNRDYNDLLISSSCVDQAKRLKQNFLLKLYKKLRSCRWPIT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKS+V+RNATIE LT AGYKGWL LIMRMDD ++IDH EYF++QQA L  EGFR+VG
Sbjct: 206 LISRKSDVNRNATIEHLTSAGYKGWLQLIMRMDDGMKIDHREYFSQQQAVLQNEGFRVVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQ 280
           VMSSHMDALS P +GTRIYKLPNP++ NIN+Q + K T+
Sbjct: 266 VMSSHMDALSAPSVGTRIYKLPNPMYLNINHQKLSKHTK 304

BLAST of MS017214 vs. NCBI nr
Match: XP_022957004.1 (uncharacterized protein At2g39920 isoform X1 [Cucurbita moschata] >XP_022957005.1 uncharacterized protein At2g39920 isoform X1 [Cucurbita moschata])

HSP 1 Score: 436.0 bits (1120), Expect = 2.4e-118
Identity = 217/276 (78.62%), Postives = 241/276 (87.32%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V+NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVNNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDSDDLLFANHDYDDLLISSSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGY GWL LIMRMDD++QI H EYF++QQAAL  EGF IVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYNGWLHLIMRMDDKMQISHGEYFSQQQAALRNEGFHIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+QN+ K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQNLSK 301

BLAST of MS017214 vs. NCBI nr
Match: XP_022978252.1 (uncharacterized protein At2g39920 isoform X2 [Cucurbita maxima])

HSP 1 Score: 434.9 bits (1117), Expect = 5.3e-118
Identity = 218/276 (78.99%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVHNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD+DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDLDDLLFANHDYDDLLI-SSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGYKGWL LIMRMDD++QI H EYF++QQAAL  EGFRIVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYKGWLHLIMRMDDKMQISHREYFSQQQAALRKEGFRIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+Q + K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQKLSK 300

BLAST of MS017214 vs. ExPASy Swiss-Prot
Match: O04195 (Uncharacterized protein At2g39920 OS=Arabidopsis thaliana OX=3702 GN=At2g39920 PE=2 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 9.3e-49
Identity = 113/279 (40.50%), Postives = 167/279 (59.86%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +++GSRY +ESG YMT L A+IFI +LVT GVL+ITLL++L+ MLQ C+N++  +VE Q+
Sbjct: 21  SELGSRYSIESGCYMTSLAASIFIASLVTFGVLMITLLIALSTMLQSCENRNIAIVEAQR 80

Query: 61  PGVGYDYCKALSVHLELNSLETDG-IPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCG 120
               + YCK LS+H +LNSL+ +  +P  C++ A+  IK G Y R+L+ T+Q+   YF  
Sbjct: 81  LDESFGYCKILSLHSQLNSLDEESELPLLCRDVALHRIKQGIYLRELNFTIQMALTYFQT 140

Query: 121 AVQVYNGRDIVLMDIDDV-LFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWP 180
              + +  D+V++DIDD  L E   Y         ++EAK  K   +L LY KLRS G+ 
Sbjct: 141 IKPMNDNCDVVVIDIDDTNLLEQDSY-----YMKYIEEAKHQKSILILALYSKLRSQGYS 200

Query: 181 LILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRI 240
           ++L+SR+ E  RNATIE+L   GY  W  LIM  +D            +Q   L  G R+
Sbjct: 201 MVLLSRRPETERNATIEQLKSRGYSDWSHLIMSRED-----------TRQKEELERGHRV 260

Query: 241 VGVMSSHMDALSGP--PIGTRIYKLPNPIHFNINYQNMR 276
           +GV+ +HMD L G       R++KLP+  + ++   N R
Sbjct: 261 IGVIGNHMDVLRGQWNWQSKRLFKLPSLTYDDVLDYNER 283

BLAST of MS017214 vs. ExPASy Swiss-Prot
Match: P27061 (Acid phosphatase 1 OS=Solanum lycopersicum OX=4081 GN=APS1 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.9e-18
Identity = 58/211 (27.49%), Postives = 100/211 (47.39%), Query Frame = 0

Query: 68  CKALSVHLELNSLET-DGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGAVQVYNG 127
           C      +E N+L     IP  C ++  +Y+    Y+ ++D        Y        +G
Sbjct: 43  CTTWRFVVETNNLSPWKTIPEECADYVKEYMVGPGYKMEIDRVSDEAGEYAKSVDLGDDG 102

Query: 128 RDIVLMDIDDVLFEN-PGYDNLLIPCSCVDEAKILK---------QKFLLQLYKKLRSCG 187
           RD+ + D+D+ L  N P Y +        D+ +  K             L+LY+++   G
Sbjct: 103 RDVWIFDVDETLLSNLPYYSDHRYGLEVFDDVEFDKWVENGTAPALGSSLKLYQEVLKLG 162

Query: 188 WPLILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGF 247
           + + L++ +SE HR+ T+E L  AG+  W  LI+R  D+       Y ++++ A++ EGF
Sbjct: 163 FKVFLLTGRSERHRSVTVENLMNAGFHDWHKLILRGSDDHGKTATTYKSERRNAMVEEGF 222

Query: 248 RIVGVMSSHMDALSGPPIGTRIYKLPNPIHF 268
           RIVG        L G  +  R +KLPNP+++
Sbjct: 223 RIVGNSGDQWSDLLGSSMSYRSFKLPNPMYY 253

BLAST of MS017214 vs. ExPASy Swiss-Prot
Match: O82122 (Vegetative storage protein 2 OS=Arabidopsis thaliana OX=3702 GN=VSP2 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.6e-10
Identity = 65/264 (24.62%), Postives = 122/264 (46.21%), Query Frame = 0

Query: 18  LTATIFIGALVTVGVL-LITLLVSLTVMLQYCQNKSEGVVEIQKPGVGYDYCKALSVHLE 77
           L+  + + A V+  V  LI L+ S T+      N +E ++E +K  + Y  C++  + +E
Sbjct: 6   LSLLLLLAATVSASVPGLIELVDSKTIF----GNVAE-LLEKEKLSINYANCRSWHLGVE 65

Query: 78  L-NSLETDGIPSFCKNFAIQYIKTG-QYERDLDSTLQVVDNYFCGAVQVYNGRDIVLMDI 137
             N ++ D +P+ CK++   Y+ T  QY+ D  +  +    Y  G     +  ++ + D+
Sbjct: 66  TSNIIDFDTVPANCKDYVEDYLITSKQYQYDSKTVCKEAYFYAKGLALKNDTVNVWIFDL 125

Query: 138 DDVLFEN-PGYDNLLIPCSCVDE----------AKILKQKFLLQLYKKLRSCGWPLILIS 197
           DD L  + P Y          D           A        L LY+ +   G   I++S
Sbjct: 126 DDTLLSSIPYYAKYGYGTEKTDPGAYWLWLGTGASTPGLPEALHLYQNIIELGIEPIILS 185

Query: 198 RKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVGVMS 257
            + ++ +N T++ L  AG   W  LI++ +    +    Y +K + +L+ +G+ IVG + 
Sbjct: 186 DRWKLWKNVTLDNLEAAGVTYWKHLILKPNGS-NLRQVVYKSKVRKSLVKKGYNIVGNIG 245

Query: 258 SHMDALSGPPIGTRIYKLPNPIHF 268
                L     G R++KLPNP+++
Sbjct: 246 DQWADLVEDTPG-RVFKLPNPLYY 262

BLAST of MS017214 vs. ExPASy Swiss-Prot
Match: P10743 (Stem 31 kDa glycoprotein OS=Glycine max OX=3847 GN=VSPB PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.2e-09
Identity = 51/212 (24.06%), Postives = 92/212 (43.40%), Query Frame = 0

Query: 68  CKALSVHLELNSLET-DGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGAVQVYNG 127
           C +  + +E +++     IP  C      YI   Q+  D  +  Q    +F  + +  + 
Sbjct: 43  CASFRLAVEAHNIRAFKTIPEECVEPTKDYINGEQFRSDSKTVNQ--QAFFYASEREVHH 102

Query: 128 RDIVLMDIDDVLFEN-PGYD---------NLLIPCSCVDEAKILKQKFLLQLYKKLRSCG 187
            DI +  ID+ +  N P Y+         N  +    V++         L+ Y KL S G
Sbjct: 103 NDIFIFGIDNTVLSNIPYYEKHGYGVEEFNETLYDEWVNKGDAPALPETLKNYNKLLSLG 162

Query: 188 WPLILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGF 247
           + ++ +S +       T   L  AG+  W  LI++    +  +   Y +  +  LL +G+
Sbjct: 163 FKIVFLSGRYLDKMAVTEANLKKAGFHTWEQLILKDPHLITPNALSYKSAMRENLLRQGY 222

Query: 248 RIVGVMSSHMDALSGPPIG-TRIYKLPNPIHF 268
           RIVG++      L G   G +R +KLPNP+++
Sbjct: 223 RIVGIIGDQWSDLLGDHRGESRTFKLPNPMYY 252

BLAST of MS017214 vs. ExPASy TrEMBL
Match: A0A6J1DWF6 (uncharacterized protein At2g39920 OS=Momordica charantia OX=3673 GN=LOC111023752 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.0e-154
Identity = 275/280 (98.21%), Postives = 276/280 (98.57%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK
Sbjct: 26  SDMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
           PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA
Sbjct: 86  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
           VQV NGRDIVLMDIDDVLFENPGYDNLLIPC CVDEAKILKQKFLLQLYKKLRSCGWPLI
Sbjct: 146 VQVNNGRDIVLMDIDDVLFENPGYDNLLIPC-CVDEAKILKQKFLLQLYKKLRSCGWPLI 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATIERLT AG KGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG
Sbjct: 206 LISRKSEVHRNATIERLTSAGCKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQI 281
           VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQI
Sbjct: 266 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRKQTQI 304

BLAST of MS017214 vs. ExPASy TrEMBL
Match: A0A6J1ISF3 (uncharacterized protein At2g39920 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478272 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.4e-119
Identity = 218/276 (78.99%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVHNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD+DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDLDDLLFANHDYDDLLISSSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGYKGWL LIMRMDD++QI H EYF++QQAAL  EGFRIVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYKGWLHLIMRMDDKMQISHREYFSQQQAALRKEGFRIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+Q + K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQKLSK 301

BLAST of MS017214 vs. ExPASy TrEMBL
Match: A0A6J1GZB6 (uncharacterized protein At2g39920 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458511 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.2e-118
Identity = 217/276 (78.62%), Postives = 241/276 (87.32%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V+NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVNNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDSDDLLFANHDYDDLLISSSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGY GWL LIMRMDD++QI H EYF++QQAAL  EGF IVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYNGWLHLIMRMDDKMQISHGEYFSQQQAALRNEGFHIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+QN+ K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQNLSK 301

BLAST of MS017214 vs. ExPASy TrEMBL
Match: A0A6J1IKM3 (uncharacterized protein At2g39920 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478272 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.6e-118
Identity = 218/276 (78.99%), Postives = 242/276 (87.68%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVHNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD+DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDLDDLLFANHDYDDLLI-SSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGYKGWL LIMRMDD++QI H EYF++QQAAL  EGFRIVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYKGWLHLIMRMDDKMQISHREYFSQQQAALRKEGFRIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+Q + K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQKLSK 300

BLAST of MS017214 vs. ExPASy TrEMBL
Match: A0A6J1GY16 (uncharacterized protein At2g39920 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458511 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.2e-117
Identity = 217/276 (78.62%), Postives = 241/276 (87.32%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +DMGS YI++SGFYMTPLTA IFIGALVTVGVLLITLLVSLTVMLQYCQN+S GVVEIQK
Sbjct: 26  SDMGSNYIIDSGFYMTPLTAAIFIGALVTVGVLLITLLVSLTVMLQYCQNRSGGVVEIQK 85

Query: 61  PGVGYDYCKALSVHLELNSLETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCGA 120
               YDYCKALS+HLELNSLETDGIPSFCK+FAIQYI+ GQYERDLDSTLQ+V+NYFC A
Sbjct: 86  LSFDYDYCKALSMHLELNSLETDGIPSFCKDFAIQYIRHGQYERDLDSTLQLVNNYFCSA 145

Query: 121 VQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWPLI 180
            +V N R+IVLMD DD+LF N  YD+LLI  SCVDEAK LKQ FLL+LYKKLRSC WP+ 
Sbjct: 146 AKVDNRREIVLMDSDDLLFANHDYDDLLI-SSCVDEAKRLKQNFLLKLYKKLRSCRWPVT 205

Query: 181 LISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRIVG 240
           LISRKSEVHRNATI+ LT AGY GWL LIMRMDD++QI H EYF++QQAAL  EGF IVG
Sbjct: 206 LISRKSEVHRNATIQHLTSAGYNGWLHLIMRMDDKMQISHGEYFSQQQAALRNEGFHIVG 265

Query: 241 VMSSHMDALSGPPIGTRIYKLPNPIHFNINYQNMRK 277
           VMSS+MDALS P +GTRIYKLPNPI FNIN+QN+ K
Sbjct: 266 VMSSYMDALSVPSVGTRIYKLPNPICFNINHQNLSK 300

BLAST of MS017214 vs. TAIR 10
Match: AT2G39920.2 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 196.1 bits (497), Expect = 3.9e-50
Identity = 114/279 (40.86%), Postives = 167/279 (59.86%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +++GSRY +ESG YMT L A+IFI +LVT GVL+ITLL++L+ MLQ C+N++  +VE Q+
Sbjct: 21  SELGSRYSIESGCYMTSLAASIFIASLVTFGVLMITLLIALSTMLQSCENRNIAIVEAQR 80

Query: 61  PGVGYDYCKALSVHLELNSLETDG-IPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCG 120
               + YCK LS+H +LNSL+ +  +P  C++ A+  IK G Y R+L+ T+Q+   YF  
Sbjct: 81  LDESFGYCKILSLHSQLNSLDEESELPLLCRDVALHRIKQGIYLRELNFTIQMALTYFQT 140

Query: 121 AVQVYNGRDIVLMDIDDV-LFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWP 180
              + +  D+V++DIDD  L E   Y         ++EAK  K   +L LY KLRS G+ 
Sbjct: 141 IKPMNDNCDVVVIDIDDTNLLEQDSY-----YMKYIEEAKHQKSILILALYSKLRSQGYS 200

Query: 181 LILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRI 240
           ++L+SR+ E  RNATIE+L   GY  W  LIMR D             +Q   L  G R+
Sbjct: 201 MVLLSRRPETERNATIEQLKSRGYSDWSHLIMRED------------TRQKEELERGHRV 260

Query: 241 VGVMSSHMDALSGP--PIGTRIYKLPNPIHFNINYQNMR 276
           +GV+ +HMD L G       R++KLP+  + ++   N R
Sbjct: 261 IGVIGNHMDVLRGQWNWQSKRLFKLPSLTYDDVLDYNER 282

BLAST of MS017214 vs. TAIR 10
Match: AT2G39920.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 195.3 bits (495), Expect = 6.6e-50
Identity = 113/279 (40.50%), Postives = 167/279 (59.86%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +++GSRY +ESG YMT L A+IFI +LVT GVL+ITLL++L+ MLQ C+N++  +VE Q+
Sbjct: 21  SELGSRYSIESGCYMTSLAASIFIASLVTFGVLMITLLIALSTMLQSCENRNIAIVEAQR 80

Query: 61  PGVGYDYCKALSVHLELNSLETDG-IPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCG 120
               + YCK LS+H +LNSL+ +  +P  C++ A+  IK G Y R+L+ T+Q+   YF  
Sbjct: 81  LDESFGYCKILSLHSQLNSLDEESELPLLCRDVALHRIKQGIYLRELNFTIQMALTYFQT 140

Query: 121 AVQVYNGRDIVLMDIDDV-LFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWP 180
              + +  D+V++DIDD  L E   Y         ++EAK  K   +L LY KLRS G+ 
Sbjct: 141 IKPMNDNCDVVVIDIDDTNLLEQDSY-----YMKYIEEAKHQKSILILALYSKLRSQGYS 200

Query: 181 LILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRI 240
           ++L+SR+ E  RNATIE+L   GY  W  LIM  +D            +Q   L  G R+
Sbjct: 201 MVLLSRRPETERNATIEQLKSRGYSDWSHLIMSRED-----------TRQKEELERGHRV 260

Query: 241 VGVMSSHMDALSGP--PIGTRIYKLPNPIHFNINYQNMR 276
           +GV+ +HMD L G       R++KLP+  + ++   N R
Sbjct: 261 IGVIGNHMDVLRGQWNWQSKRLFKLPSLTYDDVLDYNER 283

BLAST of MS017214 vs. TAIR 10
Match: AT2G39920.3 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 195.3 bits (495), Expect = 6.6e-50
Identity = 113/279 (40.50%), Postives = 167/279 (59.86%), Query Frame = 0

Query: 1   ADMGSRYIVESGFYMTPLTATIFIGALVTVGVLLITLLVSLTVMLQYCQNKSEGVVEIQK 60
           +++GSRY +ESG YMT L A+IFI +LVT GVL+ITLL++L+ MLQ C+N++  +VE Q+
Sbjct: 21  SELGSRYSIESGCYMTSLAASIFIASLVTFGVLMITLLIALSTMLQSCENRNIAIVEAQR 80

Query: 61  PGVGYDYCKALSVHLELNSLETDG-IPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYFCG 120
               + YCK LS+H +LNSL+ +  +P  C++ A+  IK G Y R+L+ T+Q+   YF  
Sbjct: 81  LDESFGYCKILSLHSQLNSLDEESELPLLCRDVALHRIKQGIYLRELNFTIQMALTYFQT 140

Query: 121 AVQVYNGRDIVLMDIDDV-LFENPGYDNLLIPCSCVDEAKILKQKFLLQLYKKLRSCGWP 180
              + +  D+V++DIDD  L E   Y         ++EAK  K   +L LY KLRS G+ 
Sbjct: 141 IKPMNDNCDVVVIDIDDTNLLEQDSY-----YMKYIEEAKHQKSILILALYSKLRSQGYS 200

Query: 181 LILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAALLYEGFRI 240
           ++L+SR+ E  RNATIE+L   GY  W  LIM  +D            +Q   L  G R+
Sbjct: 201 MVLLSRRPETERNATIEQLKSRGYSDWSHLIMSRED-----------TRQKEELERGHRV 260

Query: 241 VGVMSSHMDALSGP--PIGTRIYKLPNPIHFNINYQNMR 276
           +GV+ +HMD L G       R++KLP+  + ++   N R
Sbjct: 261 IGVIGNHMDVLRGQWNWQSKRLFKLPSLTYDDVLDYNER 283

BLAST of MS017214 vs. TAIR 10
Match: AT5G44020.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 108.6 bits (270), Expect = 8.2e-24
Identity = 66/232 (28.45%), Postives = 114/232 (49.14%), Query Frame = 0

Query: 54  GVVEIQKPGVGYDYCKALSVHLELNSL-ETDGIPSFCKNFAIQYIKTGQYERDLDSTLQV 113
           GV  ++ P +   YC++  V++ELN++ +   +P  C  F  +Y+ + QYE D++  +  
Sbjct: 43  GVTSLKAPNLN-GYCESWRVNVELNNIRDFKVVPQECVWFVQKYMTSSQYEDDVERAVDE 102

Query: 114 VDNYF---CGAVQVYNGRDIVLMDIDDVLFENPGYDNLLIPCSCVDEAKILKQKF----- 173
              Y    C   +  +G D  + DIDD L     Y        C    ++   KF     
Sbjct: 103 AILYLGKTCCEKKTCDGMDAWIFDIDDTLLSTIPYHK---SNGCFGGEQLNTTKFEEWQN 162

Query: 174 ---------LLQLYKKLRSCGWPLILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDE 233
                    +++LY ++R  G+ + LIS + E  R+AT+E L  AGY  W  L++R +D+
Sbjct: 163 SGKAPAVPHMVKLYHEIRERGFKIFLISSRKEYLRSATVENLIEAGYHSWSNLLLRGEDD 222

Query: 234 VQIDHHEYFTKQQAALLYEGFRIVGVMSSHMDALSGPPIGTRIYKLPNPIHF 268
            +    +Y    +  L   G+R+ GVM +  ++ SG P+  R +KLPN I++
Sbjct: 223 EKKSVSQYKADLRTWLTSLGYRVWGVMGAQWNSFSGCPVPKRTFKLPNSIYY 270

BLAST of MS017214 vs. TAIR 10
Match: AT1G04040.1 (HAD superfamily, subfamily IIIB acid phosphatase )

HSP 1 Score: 99.0 bits (245), Expect = 6.5e-21
Identity = 62/216 (28.70%), Postives = 113/216 (52.31%), Query Frame = 0

Query: 67  YCKALSVHLELNSL-ETDGIPSFCKNFAIQYIKTGQYERDLDSTLQVVDNYF---CGAVQ 126
           YC++  +++E++++ + D +P  C +    Y+ + QY+ D+  T+  V  +F   C +  
Sbjct: 54  YCESWRINVEVHNIRKFDVVPQECVSHIKDYMTSSQYKDDVARTVDEVILHFGSMCCSKS 113

Query: 127 VYNGRDIVLMDIDDVLFENPGY--DNLLIPCSCVDEAK----ILKQK-----FLLQLYKK 186
             +G D  + DIDD L     Y   N       ++  K    I K+K      + +LY  
Sbjct: 114 KCDGMDAWIFDIDDTLLSTIPYHKKNGFFGGEKLNSTKFEDWIQKKKAPAVPHMKKLYHD 173

Query: 187 LRSCGWPLILISRKSEVHRNATIERLTCAGYKGWLLLIMRMDDEVQIDHHEYFTKQQAAL 246
           +R  G  + LIS + E  R+AT++ L  AGY GW  L++R  ++ Q +  +Y ++++  L
Sbjct: 174 IRERGIKIFLISSRKEYLRSATVDNLIQAGYYGWSNLMLRGLEDQQKEVKQYKSEKRKWL 233

Query: 247 LYEGFRIVGVMSSHMDALSGPPIGTRIYKLPNPIHF 268
           +  G+R+ GVM     + +G P+  R +KLPN I++
Sbjct: 234 MSLGYRVWGVMGDQWSSFAGCPLPRRTFKLPNSIYY 269