MS017184 (gene) Bitter gourd (TR) v1

Overview
NameMS017184
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionOmega-3 fatty acid desaturase
Locationscaffold33: 421486 .. 423552 (+)
RNA-Seq ExpressionMS017184
SyntenyMS017184
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGTGGGTCTTGTCTGAATGTGGGGTTAAGCCCCTCCCCAGGATCTACCCTCTGCCTTCCAGGACTGGGCTTGCTTCCAAGGCTTTCATGGAGATTAGGCAGCCATTGTCGGCTTCTAGAGCTCACATTCTGCGCTCTTCTTTGGGTCTTTGGAAGGGGAAGAGTTGGGGGCTGAATGTGAGTGCTCCATTGAGAGTTGCTTCTGTGGATGAAGATAATGAGAGGGAGAGAGTTGATGTGGTTGGAGACGATGGCGGGTTCGACCCCGGTGCTGCTCCGCCGTTTCGGCTGGCCGACGTTCGGGCCGCCATTCCGAATCATTGTTGGGTTAAGGATCCTTGGAAATCAATGAGCTATGTTGTGAGGGATGTGGTTGTTGTGCTTGGATTGGCGGCTGTTGCTGCTTACTTCAACAATTGGGTTGTTTGGCCTCTTTACTGGTTTGCTCAGGGCACCATGTTTTGGGCTCTTTTTGTTCTTGGTCATGATTGGTAATTTTCTCTGTTCAAGAAATTGAATCTGTCCTCACTGTCTTCCAATGCTTTTAATTCCTTTGCCTGGATTTTCTAATTCTTGGTTGGATTTGCAGTGGCCATGGAAGTTTCTCTAACAACCCAAATCTGAATAGTGTTGTTGGTCATCTTCTTCATTCTTCAATTCTAGTTCCATATCATGGATGGTTAGTTCCCTGATTACCTTATCAAAGACGAATGTCTTCCAATCGTTGGTTGATGTTGATCATTATTCTCTGCAGCTTTAACTCTCTATCAGAAATGTAAAATAGTTCTGTTTATCTTGTTTAACTCAGGAGAATAAGCCACAGAACTCATCACCAGAACCACGGTCATGTCGAAAACGACGAATCATGGCATCCTGTAAGTTCTTTATCTCTAGGAAGCAGTTTGTAACAAGAAGAGGCTATTTTTTTCAGTGTCATTTAATGGCAATATGTTCATATCTGTTTTGCAGTTGTCTGAGAAAATTTATAGGAGTTTGGATAAAGCAACTAAAACATTGAGGTTCACCTTGCCTTTCCCCATGCTTGCATATCCTGTTTATCTGGTTAGTACACTCTGTTTCTTCACCCTTCTTTATGAACTTCAATGCTATGCCAGTGTTTTGATGGATTATAATGCATCCTGTTGGTGGCATTACAGTGGAATAGAAGCCCTGGAAAGAAGGGCTCTCATTTTGACCCGAGCAGCGATTTGTTCGTACCGAGCGAGCGGAATGATATCATTACATCCACGGCCTGTTGGGTTGCAATGGTTGCTCTGCTTGGGTGTCTGTCCGTAGTGATGGGTCCCCTCCAACTCCTCAAACTCTATGGCATTCCCTACCTGGTGAGTTCAGCTCCGGTCACGAATCCACGACGAATGCTTTTCAGTATTCATTAAGACTTCTACGTTTAGTCGACTTTTTTTCAATACCATCATTCCCTTGTGCACTCTTTTGAATGTTCTTCTTCTATATGCAGCTGTTTGTCATGTGGCTGGATGTGGTCACGTACCTGCATCACCACGGTCACGACGACAAACTTCCTTGGTATCGTGGAGAGGTATGCTGATCAAAGCTTTTCAAGTGTTCGATGTCAAATTTCAAGAACTAACACATAAAAGTATCATGTTTGACACAGTTTTATTAACTTGGCTGGCTCTGAATTTCATGATCAAACAGGAATGGAGTTATTTAAGAGGAGGATTAACCACTCTCGATCGCGATTACGGATGGATCAACAACATCCACCATGACATTGGAACCCATGTCATTCACCATCTCTTCCCTCAGATCCCACACTATCATTTAATAGAAGCTGTGAGTATACTTGAAATCTAAACTTATCTTTGTACTGCATCTTCAAAATTCTCACTGAAAACTGTAATCTAATGGTTTCAGACCGAGGCAGCAAAGCCAGTGCTCGGGAAGTACTATCGGGAACCGAAGAAATCCTTGCCTCTCCCACTTCACTTGTTGGGAGTTCTCGTAAAAAGCATGAGGAAGGATCACTATGTGAGCGATTCTGGGGACATTGTATATTATCAGACTGACCCCAACCTCTCT

mRNA sequence

ATGGCGAGGTGGGTCTTGTCTGAATGTGGGGTTAAGCCCCTCCCCAGGATCTACCCTCTGCCTTCCAGGACTGGGCTTGCTTCCAAGGCTTTCATGGAGATTAGGCAGCCATTGTCGGCTTCTAGAGCTCACATTCTGCGCTCTTCTTTGGGTCTTTGGAAGGGGAAGAGTTGGGGGCTGAATGTGAGTGCTCCATTGAGAGTTGCTTCTGTGGATGAAGATAATGAGAGGGAGAGAGTTGATGTGGTTGGAGACGATGGCGGGTTCGACCCCGGTGCTGCTCCGCCGTTTCGGCTGGCCGACGTTCGGGCCGCCATTCCGAATCATTGTTGGGTTAAGGATCCTTGGAAATCAATGAGCTATGTTGTGAGGGATGTGGTTGTTGTGCTTGGATTGGCGGCTGTTGCTGCTTACTTCAACAATTGGGTTGTTTGGCCTCTTTACTGGTTTGCTCAGGGCACCATGTTTTGGGCTCTTTTTGTTCTTGGTCATGATTGTGGCCATGGAAGTTTCTCTAACAACCCAAATCTGAATAGTGTTGTTGGTCATCTTCTTCATTCTTCAATTCTAGTTCCATATCATGGATGGAGAATAAGCCACAGAACTCATCACCAGAACCACGGTCATGTCGAAAACGACGAATCATGGCATCCTTTGTCTGAGAAAATTTATAGGAGTTTGGATAAAGCAACTAAAACATTGAGGTTCACCTTGCCTTTCCCCATGCTTGCATATCCTGTTTATCTGTGGAATAGAAGCCCTGGAAAGAAGGGCTCTCATTTTGACCCGAGCAGCGATTTGTTCGTACCGAGCGAGCGGAATGATATCATTACATCCACGGCCTGTTGGGTTGCAATGGTTGCTCTGCTTGGGTGTCTGTCCGTAGTGATGGGTCCCCTCCAACTCCTCAAACTCTATGGCATTCCCTACCTGCTGTTTGTCATGTGGCTGGATGTGGTCACGTACCTGCATCACCACGGTCACGACGACAAACTTCCTTGGTATCGTGGAGAGGAATGGAGTTATTTAAGAGGAGGATTAACCACTCTCGATCGCGATTACGGATGGATCAACAACATCCACCATGACATTGGAACCCATGTCATTCACCATCTCTTCCCTCAGATCCCACACTATCATTTAATAGAAGCTACCGAGGCAGCAAAGCCAGTGCTCGGGAAGTACTATCGGGAACCGAAGAAATCCTTGCCTCTCCCACTTCACTTGTTGGGAGTTCTCGTAAAAAGCATGAGGAAGGATCACTATGTGAGCGATTCTGGGGACATTGTATATTATCAGACTGACCCCAACCTCTCT

Coding sequence (CDS)

ATGGCGAGGTGGGTCTTGTCTGAATGTGGGGTTAAGCCCCTCCCCAGGATCTACCCTCTGCCTTCCAGGACTGGGCTTGCTTCCAAGGCTTTCATGGAGATTAGGCAGCCATTGTCGGCTTCTAGAGCTCACATTCTGCGCTCTTCTTTGGGTCTTTGGAAGGGGAAGAGTTGGGGGCTGAATGTGAGTGCTCCATTGAGAGTTGCTTCTGTGGATGAAGATAATGAGAGGGAGAGAGTTGATGTGGTTGGAGACGATGGCGGGTTCGACCCCGGTGCTGCTCCGCCGTTTCGGCTGGCCGACGTTCGGGCCGCCATTCCGAATCATTGTTGGGTTAAGGATCCTTGGAAATCAATGAGCTATGTTGTGAGGGATGTGGTTGTTGTGCTTGGATTGGCGGCTGTTGCTGCTTACTTCAACAATTGGGTTGTTTGGCCTCTTTACTGGTTTGCTCAGGGCACCATGTTTTGGGCTCTTTTTGTTCTTGGTCATGATTGTGGCCATGGAAGTTTCTCTAACAACCCAAATCTGAATAGTGTTGTTGGTCATCTTCTTCATTCTTCAATTCTAGTTCCATATCATGGATGGAGAATAAGCCACAGAACTCATCACCAGAACCACGGTCATGTCGAAAACGACGAATCATGGCATCCTTTGTCTGAGAAAATTTATAGGAGTTTGGATAAAGCAACTAAAACATTGAGGTTCACCTTGCCTTTCCCCATGCTTGCATATCCTGTTTATCTGTGGAATAGAAGCCCTGGAAAGAAGGGCTCTCATTTTGACCCGAGCAGCGATTTGTTCGTACCGAGCGAGCGGAATGATATCATTACATCCACGGCCTGTTGGGTTGCAATGGTTGCTCTGCTTGGGTGTCTGTCCGTAGTGATGGGTCCCCTCCAACTCCTCAAACTCTATGGCATTCCCTACCTGCTGTTTGTCATGTGGCTGGATGTGGTCACGTACCTGCATCACCACGGTCACGACGACAAACTTCCTTGGTATCGTGGAGAGGAATGGAGTTATTTAAGAGGAGGATTAACCACTCTCGATCGCGATTACGGATGGATCAACAACATCCACCATGACATTGGAACCCATGTCATTCACCATCTCTTCCCTCAGATCCCACACTATCATTTAATAGAAGCTACCGAGGCAGCAAAGCCAGTGCTCGGGAAGTACTATCGGGAACCGAAGAAATCCTTGCCTCTCCCACTTCACTTGTTGGGAGTTCTCGTAAAAAGCATGAGGAAGGATCACTATGTGAGCGATTCTGGGGACATTGTATATTATCAGACTGACCCCAACCTCTCT

Protein sequence

MARWVLSECGVKPLPRIYPLPSRTGLASKAFMEIRQPLSASRAHILRSSLGLWKGKSWGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSMRKDHYVSDSGDIVYYQTDPNLS
Homology
BLAST of MS017184 vs. NCBI nr
Match: XP_038892287.1 (omega-3 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 849.0 bits (2192), Expect = 1.9e-242
Identity = 398/445 (89.44%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYP PSR G+ASK FM  EIRQPL AS+AH+LRSSLGL  +GKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGIASKTFMVSEIRQPLLASKAHVLRSSLGLSTRGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVG---DDGGFDPGAAPPFRLADVRAAIPNHCWVKD 120
           W LNVSAP+RVAS+DEDNE+ERVD +    D+G FDP AAPPFRLAD+RAAIP HCWVKD
Sbjct: 61  WALNVSAPVRVASIDEDNEKERVDGINGVEDEGDFDPAAAPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNN 180
           PWKSMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 121 PWKSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTL 240
           P LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKAT+TL
Sbjct: 181 PKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLS 300
           RFTLPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITSTACW AM+ALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360
           VVMGPLQ+LKLYGIPYL+FVMWLD VTYLHHHGH+DKLPWYRGEEWSYLRGGLTTLDRDY
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHEDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLV 420
           GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPVLGKYYREP+KS PLP HLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYREPEKSWPLPFHLLGVLV 420

Query: 421 KSMRKDHYVSDSGDIVYYQTDPNLS 440
           KS+RKDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KSLRKDHFVSDSGDIVYYQTDPDLS 445

BLAST of MS017184 vs. NCBI nr
Match: XP_008465212.1 (PREDICTED: omega-3 fatty acid desaturase, chloroplastic [Cucumis melo] >KAA0052881.1 omega-3 fatty acid desaturase [Cucumis melo var. makuwa] >TYK14479.1 omega-3 fatty acid desaturase [Cucumis melo var. makuwa])

HSP 1 Score: 845.9 bits (2184), Expect = 1.6e-241
Identity = 397/445 (89.21%), Postives = 417/445 (93.71%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYP PSR G ASK FM  EIRQPL  S+AH+LRSSLG+  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVG---DDGGFDPGAAPPFRLADVRAAIPNHCWVKD 120
           W LNVS P+ +ASVDEDNERERVD V    D+GGFDP A PPFRLAD+RAAIP HCWVKD
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNN 180
           PW+SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTL 240
           PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK+YRSLDKAT+TL
Sbjct: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLS 300
           RFTLPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITSTACW AM+ALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360
           VVMGPLQ+LKLYGIPYL+FVMWLD VTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLV 420
           GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPV GKYYREP+KS PLP HLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVFGKYYREPEKSWPLPFHLLGVLV 420

Query: 421 KSMRKDHYVSDSGDIVYYQTDPNLS 440
           KS+RKDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KSLRKDHFVSDSGDIVYYQTDPDLS 445

BLAST of MS017184 vs. NCBI nr
Match: NP_001274404.1 (omega-3 fatty acid desaturase [Cucumis sativus] >ACE80931.1 omega-3 fatty acid desaturase [Cucumis sativus] >KGN50667.1 hypothetical protein Csa_005845 [Cucumis sativus])

HSP 1 Score: 841.3 bits (2172), Expect = 3.9e-240
Identity = 392/445 (88.09%), Postives = 417/445 (93.71%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYPLPSR G ASK FM  EIRQPL AS+AH+LRSSLGL  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPLPSRNGFASKTFMVSEIRQPLLASKAHVLRSSLGLSTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVG---DDGGFDPGAAPPFRLADVRAAIPNHCWVKD 120
           W LNVS P+ VASVDEDNERERV+ V    D+G FDP A PPFRLAD+RAAIP HCWV+D
Sbjct: 61  WALNVSTPVAVASVDEDNERERVNEVNGVEDEGSFDPAAPPPFRLADIRAAIPKHCWVRD 120

Query: 121 PWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNN 180
           PW+SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTL 240
           PNLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKAT+TL
Sbjct: 181 PNLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLS 300
           RF LPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITSTACW AM+ALLGCLS
Sbjct: 241 RFALPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360
           VVMGPLQ++KLYGIPY++FVMWLD VTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY
Sbjct: 301 VVMGPLQIIKLYGIPYMMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLV 420
           GWINNIHHDIGTHV+HHLFPQIPHYHLIEATEA KP+ GKYYREP+KS PLP HLLGVLV
Sbjct: 361 GWINNIHHDIGTHVVHHLFPQIPHYHLIEATEATKPIFGKYYREPEKSWPLPFHLLGVLV 420

Query: 421 KSMRKDHYVSDSGDIVYYQTDPNLS 440
           +S+RKDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 RSLRKDHFVSDSGDIVYYQTDPDLS 445

BLAST of MS017184 vs. NCBI nr
Match: XP_022995701.1 (omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 828.6 bits (2139), Expect = 2.6e-236
Identity = 387/442 (87.56%), Postives = 414/442 (93.67%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYP PSR GLAS+ +M  EIR     S+ + LRSSL L  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPRPSRNGLASRTYMVSEIRFSSVPSKPNFLRSSLSLSTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNVSAP RVASVDEDNE+++VD V ++GGFDP AAPPFRLAD+RAAIP HCWVKDPW+
Sbjct: 61  WALNVSAPARVASVDEDNEKDKVDGVENEGGFDPAAAPPFRLADIRAAIPKHCWVKDPWR 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+R+LDKAT+TLRFT
Sbjct: 181 NSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRTLDKATRTLRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITST CW AM+ALLGCLSVVM
Sbjct: 241 LPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLSVVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GPLQ+LKLYGIPY++FVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSM 420
           NNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPVLGKYYR+P+KS PLP HLLGVLVKSM
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLVKSM 420

Query: 421 RKDHYVSDSGDIVYYQTDPNLS 440
           +KDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KKDHFVSDSGDIVYYQTDPDLS 442

BLAST of MS017184 vs. NCBI nr
Match: XP_023547843.1 (omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828.2 bits (2138), Expect = 3.4e-236
Identity = 386/442 (87.33%), Postives = 413/442 (93.44%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVK LPRIYP PSR GLAS+ +M  EIR     S+ H LRSSL L  K KS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTYMVSEIRSSSVPSKPHFLRSSLSLSTKRKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNVSAP+RVASVDEDNE+++VD V ++GGFDP AAPPFRLAD+RAAIP HCWVKDPW+
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVDGVENEGGFDPAAAPPFRLADIRAAIPKHCWVKDPWR 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++LDKATKTLRFT
Sbjct: 181 NSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATKTLRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYP+YLW+RSPGKKGSHFDP+SDLFVPSERNDIITST CW AM+ALLGCLSVVM
Sbjct: 241 LPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLSVVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GPLQ+LKLYGIPY++FVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSM 420
           NNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPVLGKYYR+P+KS PLP HLLGVLVKSM
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLVKSM 420

Query: 421 RKDHYVSDSGDIVYYQTDPNLS 440
           +KDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KKDHFVSDSGDIVYYQTDPDLS 442

BLAST of MS017184 vs. ExPASy Swiss-Prot
Match: P48620 (Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum OX=4182 GN=FAD7 PE=2 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 6.5e-206
Identity = 336/443 (75.85%), Postives = 376/443 (84.88%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTG--LASKAFMEIR-QPLSASRAHILRSSLGLWKGKS 60
           MA WVLSECG++PLPR+YP P RTG  L +    ++R              S G+ + K+
Sbjct: 1   MASWVLSECGLRPLPRVYPKP-RTGHPLLNSNPTKLRFSRTDLGNGSSFCLSSGILREKN 60

Query: 61  WGLNVSAPLRVASVDEDNE-RERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPW 120
           W L VSAPLRV  V+E+ E +E   V+     FDPGA PPF+L+D+R AIP HCWVKDPW
Sbjct: 61  WALRVSAPLRVLQVEEEEENKEGERVINGGEEFDPGAPPPFKLSDIREAIPKHCWVKDPW 120

Query: 121 KSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPN 180
           +SM YVVRDV VV GLAAVAAYFNNWVVWPLYWFAQ TMFWALFVLGHDCGHGSFSN+P 
Sbjct: 121 RSMGYVVRDVAVVFGLAAVAAYFNNWVVWPLYWFAQSTMFWALFVLGHDCGHGSFSNDPK 180

Query: 181 LNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRF 240
           LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++LD ATK LRF
Sbjct: 181 LNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKNLDTATKKLRF 240

Query: 241 TLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVV 300
           TLPFP+LAYP+YLW+RSPGK+GSHF P SDLFVP+E+ D+ITST CW AM+ALL  LS V
Sbjct: 241 TLPFPLLAYPIYLWSRSPGKQGSHFHPDSDLFVPNEKKDVITSTVCWTAMLALLVGLSFV 300

Query: 301 MGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGW 360
           +GP+QLLKLYGIPYL  VMWLD+VTYLHHHGH+DKLPWYRG+EWSYLRGGLTTLDRDYGW
Sbjct: 301 IGPVQLLKLYGIPYLGNVMWLDLVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGW 360

Query: 361 INNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKS 420
           INNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKS PLP HLLG L +S
Sbjct: 361 INNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSAPLPFHLLGDLTRS 420

Query: 421 MRKDHYVSDSGDIVYYQTDPNLS 440
           +++DHYVSD GD+VYYQTDP L+
Sbjct: 421 LKRDHYVSDVGDVVYYQTDPQLT 442

BLAST of MS017184 vs. ExPASy Swiss-Prot
Match: P48619 (Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis OX=3988 GN=FAD7A-1 PE=2 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 4.2e-205
Identity = 341/452 (75.44%), Postives = 382/452 (84.51%), Query Frame = 0

Query: 2   ARWVLSECGVKPLPRIYPLPSRTGLASKA--FMEIRQPLSASRAHILRSS--LGLW---K 61
           A WVLSECG++PLPRIY  P R G  SK    +++R+ L  S+++ L SS  +  W   K
Sbjct: 3   AGWVLSECGLRPLPRIYSRP-RIGFTSKTTNLLKLRE-LPDSKSYNLCSSFKVSSWSNSK 62

Query: 62  GKSWGLNVSAPLRVASVDEDNERERVDVVG----DDGG---FDPGAAPPFRLADVRAAIP 121
             +W LNV+ P+ V++V  +++RER +  G    D+G    FD GA PPF LAD+RAAIP
Sbjct: 63  QSNWALNVAVPVNVSTVSGEDDREREEFNGIVNVDEGKGEFFDAGAPPPFTLADIRAAIP 122

Query: 122 NHCWVKDPWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCG 181
            HCWVK+PW+SMSYV+RDVVVV GLAAVAAYFNNWV WPLYWF QGTMFWALFVLGHDCG
Sbjct: 123 KHCWVKNPWRSMSYVLRDVVVVFGLAAVAAYFNNWVAWPLYWFCQGTMFWALFVLGHDCG 182

Query: 182 HGSFSNNPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSL 241
           HGSFSNNP LNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI++SL
Sbjct: 183 HGSFSNNPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFKSL 242

Query: 242 DKATKTLRFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMV 301
           D  TKTLRF+LPFPMLAYP YLW+RSPGKKGSHF P S LFVP ER DIITSTACW AM 
Sbjct: 243 DNVTKTLRFSLPFPMLAYPFYLWSRSPGKKGSHFHPDSGLFVPKERKDIITSTACWTAMA 302

Query: 302 ALLGCLSVVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGL 361
           ALL  L+  MGP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+ WSYLRGGL
Sbjct: 303 ALLVYLNFSMGPVQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKAWSYLRGGL 362

Query: 362 TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPL 421
           TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHL+EATEAAKPV+GKYYREPKKS PLPL
Sbjct: 363 TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVMGKYYREPKKSGPLPL 422

Query: 422 HLLGVLVKSMRKDHYVSDSGDIVYYQTDPNLS 440
           HLLG LV+SM++DHYVSD+GD+VYYQ DP LS
Sbjct: 423 HLLGSLVRSMKEDHYVSDTGDVVYYQKDPKLS 452

BLAST of MS017184 vs. ExPASy Swiss-Prot
Match: P48622 (Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD8 PE=1 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 5.3e-200
Identity = 323/442 (73.08%), Postives = 364/442 (82.35%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLAS--KAFMEIRQPLSASRAHILRSSLGLW-KGKS 60
           MA  VLSECG +PLPR YP    T  AS  K   +   PL    + +L S  G + K ++
Sbjct: 1   MASSVLSECGFRPLPRFYP-KHTTSFASNPKPTFKFNPPLKPP-SSLLNSRYGFYSKTRN 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNV+ PL       + + ER         FDPGA PPF LAD+RAAIP HCWVK+PW 
Sbjct: 61  WALNVATPLTTLQSPSEEDTER---------FDPGAPPPFNLADIRAAIPKHCWVKNPWM 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDV +V GLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSV GHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY++L+K T+  RFT
Sbjct: 181 NSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMFRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYP YLWNRSPGK+GSH+ P SDLF+P E+ D++TSTACW AM ALL CL+ VM
Sbjct: 241 LPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+EWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSM 420
           NNIHHDIGTHVIHHLFPQIPHYHL+EATEAAKPVLGKYYREPK S PLPLHLLG L+KSM
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPKNSGPLPLHLLGSLIKSM 420

Query: 421 RKDHYVSDSGDIVYYQTDPNLS 440
           ++DH+VSD+GD+VYY+ DP L+
Sbjct: 421 KQDHFVSDTGDVVYYEADPKLN 431

BLAST of MS017184 vs. ExPASy Swiss-Prot
Match: P48621 (Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 GN=FAD7 PE=2 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 4.2e-197
Identity = 321/453 (70.86%), Postives = 373/453 (82.34%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKA-----FMEIRQPLSASRAHILRSSLGLWKG 60
           MA W   +CG+KPL  + P P RTG A  +     F++  + ++  +   LR +L   + 
Sbjct: 1   MATWYHQKCGLKPLAPVIPRP-RTGAALSSTSRVEFLDTNKVVAGPKFQPLRCNL---RE 60

Query: 61  KSWGLNVSAPLRVASVDEDNERERVDVVGDDGG--------FDPGAAPPFRLADVRAAIP 120
           ++WGL VSAPLRVAS++E  E++ VD+     G        FDPGA PPF LAD+RAAIP
Sbjct: 61  RNWGLKVSAPLRVASIEE--EQKSVDLTNGTNGVEHEKLPEFDPGAPPPFNLADIRAAIP 120

Query: 121 NHCWVKDPWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCG 180
            HCWVKDPW+SMSYVVRDV+ V GLAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCG
Sbjct: 121 KHCWVKDPWRSMSYVVRDVIAVFGLAAAAAYLNNWLVWPLYWAAQGTMFWALFVLGHDCG 180

Query: 181 HGSFSNNPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSL 240
           HGSFSNN  LNSVVGHLLHSSILVPYHGWRISHRTHHQ+HGH ENDESWHPL EK++RSL
Sbjct: 181 HGSFSNNSKLNSVVGHLLHSSILVPYHGWRISHRTHHQHHGHAENDESWHPLPEKLFRSL 240

Query: 241 DKATKTLRFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMV 300
           D  T+ LRFT PFP+LA+PVYL++RSPGK GSHFDPSSDLFVP+ER D+ITSTACW AM+
Sbjct: 241 DTVTRMLRFTAPFPLLAFPVYLFSRSPGKTGSHFDPSSDLFVPNERKDVITSTACWAAML 300

Query: 301 ALLGCLSVVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGL 360
            LL  L  VMGP+QLLKLYG+PY++FVMWLD+VTYLHHHGH+DKLPWYRG+EWSYLRGGL
Sbjct: 301 GLLVGLGFVMGPIQLLKLYGVPYVIFVMWLDLVTYLHHHGHEDKLPWYRGKEWSYLRGGL 360

Query: 361 TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKS-LPLP 420
           TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHL+EATEAAKPV GKYYREPKKS  PLP
Sbjct: 361 TTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVFGKYYREPKKSAAPLP 420

Query: 421 LHLLGVLVKSMRKDHYVSDSGDIVYYQTDPNLS 440
            HL+G +++S + DH+VSD+GD+VYYQTD  ++
Sbjct: 421 FHLIGEIIRSFKTDHFVSDTGDVVYYQTDSKIN 447

BLAST of MS017184 vs. ExPASy Swiss-Prot
Match: Q56VS4 (sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Helianthus annuus OX=4232 GN=FAD7 PE=1 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 6.1e-196
Identity = 320/449 (71.27%), Postives = 369/449 (82.18%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFMEI---RQPLSASRAHILRSSLGLWKGKS 60
           MA  VLS C +KP  +  P+P++  + + + + +   + P+ +   H             
Sbjct: 1   MAGLVLSGCAIKPFSQSLPIPTKRFITNPSNINLLHPKDPIFSPNFHGF---------SR 60

Query: 61  WGLNVSAPLRVASVDE-----DNERERVDV--VGDDGGFDPGAAPPFRLADVRAAIPNHC 120
           W + VSAPLR+ S+D+     D ERER+    V ++  FD GA PPF+LAD+RAAIP  C
Sbjct: 61  WAVKVSAPLRIPSIDQQDLDLDLERERISSLDVQEEEIFDAGAPPPFKLADIRAAIPKRC 120

Query: 121 WVKDPWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGS 180
           WVKDPW+SMSYVVRDV +VLGLAA AA+ NNW+VWPLYW AQGTMFWALFVLGHDCGHGS
Sbjct: 121 WVKDPWRSMSYVVRDVAIVLGLAAAAAHLNNWLVWPLYWAAQGTMFWALFVLGHDCGHGS 180

Query: 181 FSNNPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKA 240
           FSNN  LNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL+EK ++SLD  
Sbjct: 181 FSNNAKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLTEKTFKSLDWI 240

Query: 241 TKTLRFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALL 300
           T+TLRFTLPFPMLAYP YLWNRSPGK GSHFDPSSDLFVP+E+ D+ITST CW  M+ALL
Sbjct: 241 TRTLRFTLPFPMLAYPFYLWNRSPGKSGSHFDPSSDLFVPAEQKDVITSTICWTTMLALL 300

Query: 301 GCLSVVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTL 360
             L+ V+GP+Q+LKLYGIPYL+ VMWLD VTYLHHHGH+DKLPWYRG+EWSYLRGGLTT+
Sbjct: 301 FGLNFVVGPVQMLKLYGIPYLINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTI 360

Query: 361 DRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLL 420
           DRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKS P+P HLL
Sbjct: 361 DRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSSPIPFHLL 420

Query: 421 GVLVKSMRKDHYVSDSGDIVYYQTDPNLS 440
           G LV+S++KDHYVSD+GD++YYQTD  LS
Sbjct: 421 GELVRSLKKDHYVSDTGDVLYYQTDDKLS 440

BLAST of MS017184 vs. ExPASy TrEMBL
Match: A0A5A7UH66 (Omega-3 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold186G001460 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.6e-242
Identity = 397/445 (89.21%), Postives = 417/445 (93.71%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYP PSR G ASK FM  EIRQPL  S+AH+LRSSLG+  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVG---DDGGFDPGAAPPFRLADVRAAIPNHCWVKD 120
           W LNVS P+ +ASVDEDNERERVD V    D+GGFDP A PPFRLAD+RAAIP HCWVKD
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNN 180
           PW+SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTL 240
           PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK+YRSLDKAT+TL
Sbjct: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLS 300
           RFTLPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITSTACW AM+ALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360
           VVMGPLQ+LKLYGIPYL+FVMWLD VTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLV 420
           GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPV GKYYREP+KS PLP HLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVFGKYYREPEKSWPLPFHLLGVLV 420

Query: 421 KSMRKDHYVSDSGDIVYYQTDPNLS 440
           KS+RKDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KSLRKDHFVSDSGDIVYYQTDPDLS 445

BLAST of MS017184 vs. ExPASy TrEMBL
Match: A0A1S3CNS0 (omega-3 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502871 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 7.6e-242
Identity = 397/445 (89.21%), Postives = 417/445 (93.71%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYP PSR G ASK FM  EIRQPL  S+AH+LRSSLG+  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVG---DDGGFDPGAAPPFRLADVRAAIPNHCWVKD 120
           W LNVS P+ +ASVDEDNERERVD V    D+GGFDP A PPFRLAD+RAAIP HCWVKD
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVEDEGGFDPAAPPPFRLADIRAAIPKHCWVKD 120

Query: 121 PWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNN 180
           PW+SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTL 240
           PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK+YRSLDKAT+TL
Sbjct: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLS 300
           RFTLPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITSTACW AM+ALLGCLS
Sbjct: 241 RFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360
           VVMGPLQ+LKLYGIPYL+FVMWLD VTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY
Sbjct: 301 VVMGPLQILKLYGIPYLMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLV 420
           GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPV GKYYREP+KS PLP HLLGVLV
Sbjct: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVFGKYYREPEKSWPLPFHLLGVLV 420

Query: 421 KSMRKDHYVSDSGDIVYYQTDPNLS 440
           KS+RKDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KSLRKDHFVSDSGDIVYYQTDPDLS 445

BLAST of MS017184 vs. ExPASy TrEMBL
Match: B3VA19 (Omega-3 fatty acid desaturase OS=Cucumis sativus OX=3659 GN=fad7 PE=2 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 1.9e-240
Identity = 392/445 (88.09%), Postives = 417/445 (93.71%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYPLPSR G ASK FM  EIRQPL AS+AH+LRSSLGL  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPLPSRNGFASKTFMVSEIRQPLLASKAHVLRSSLGLSTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVG---DDGGFDPGAAPPFRLADVRAAIPNHCWVKD 120
           W LNVS P+ VASVDEDNERERV+ V    D+G FDP A PPFRLAD+RAAIP HCWV+D
Sbjct: 61  WALNVSTPVAVASVDEDNERERVNEVNGVEDEGSFDPAAPPPFRLADIRAAIPKHCWVRD 120

Query: 121 PWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNN 180
           PW+SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+
Sbjct: 121 PWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSND 180

Query: 181 PNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTL 240
           PNLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKAT+TL
Sbjct: 181 PNLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTL 240

Query: 241 RFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLS 300
           RF LPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITSTACW AM+ALLGCLS
Sbjct: 241 RFALPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLS 300

Query: 301 VVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360
           VVMGPLQ++KLYGIPY++FVMWLD VTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY
Sbjct: 301 VVMGPLQIIKLYGIPYMMFVMWLDAVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDY 360

Query: 361 GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLV 420
           GWINNIHHDIGTHV+HHLFPQIPHYHLIEATEA KP+ GKYYREP+KS PLP HLLGVLV
Sbjct: 361 GWINNIHHDIGTHVVHHLFPQIPHYHLIEATEATKPIFGKYYREPEKSWPLPFHLLGVLV 420

Query: 421 KSMRKDHYVSDSGDIVYYQTDPNLS 440
           +S+RKDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 RSLRKDHFVSDSGDIVYYQTDPDLS 445

BLAST of MS017184 vs. ExPASy TrEMBL
Match: A0A6J1K2M7 (omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491157 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.3e-236
Identity = 387/442 (87.56%), Postives = 414/442 (93.67%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVKPLPRIYP PSR GLAS+ +M  EIR     S+ + LRSSL L  KGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPRPSRNGLASRTYMVSEIRFSSVPSKPNFLRSSLSLSTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNVSAP RVASVDEDNE+++VD V ++GGFDP AAPPFRLAD+RAAIP HCWVKDPW+
Sbjct: 61  WALNVSAPARVASVDEDNEKDKVDGVENEGGFDPAAAPPFRLADIRAAIPKHCWVKDPWR 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+R+LDKAT+TLRFT
Sbjct: 181 NSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRTLDKATRTLRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYPVYLW+RSPGKKGSHFDP+SDLFVPSERNDIITST CW AM+ALLGCLSVVM
Sbjct: 241 LPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLSVVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GPLQ+LKLYGIPY++FVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSM 420
           NNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPVLGKYYR+P+KS PLP HLLGVLVKSM
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLVKSM 420

Query: 421 RKDHYVSDSGDIVYYQTDPNLS 440
           +KDH+VSDSGDIVYYQTDP+LS
Sbjct: 421 KKDHFVSDSGDIVYYQTDPDLS 442

BLAST of MS017184 vs. ExPASy TrEMBL
Match: A0A6J1FSG4 (omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447744 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.2e-236
Identity = 385/442 (87.10%), Postives = 412/442 (93.21%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLASKAFM--EIRQPLSASRAHILRSSLGL-WKGKS 60
           MARWVLSECGVK LPRIYP PSR GLAS+  M  EIR     S+ H LRSSL L  KGKS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTCMVSEIRSSSVPSKPHFLRSSLSLSTKGKS 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNVSAP+RVASVDEDNE+++VD V ++GGFDP AAPPFRLAD+RAAIP HCWVKDPW+
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVDGVENEGGFDPAAAPPFRLADIRAAIPKHCWVKDPWR 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDVVVV GLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++LDKATKTLRFT
Sbjct: 181 NSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATKTLRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYP+YLW+RSPGKKGSHFDP+SDLFVPSERNDIITST CW AM+ALLGCLS VM
Sbjct: 241 LPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGCLSAVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GPLQ+LKLYGIPY++FVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPLQILKLYGIPYVMFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSM 420
           NNIHHDIGTHVIHHLFPQIPHYHLIEATEA KPVLGKYYR+P+KS PLP HLLGVLVKSM
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEATKPVLGKYYRQPEKSWPLPFHLLGVLVKSM 420

Query: 421 RKDHYVSDSGDIVYYQTDPNLS 440
           +KDH+VS+SGDIVYYQTDP+LS
Sbjct: 421 KKDHFVSNSGDIVYYQTDPDLS 442

BLAST of MS017184 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 698.4 bits (1801), Expect = 3.8e-201
Identity = 323/442 (73.08%), Postives = 364/442 (82.35%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLAS--KAFMEIRQPLSASRAHILRSSLGLW-KGKS 60
           MA  VLSECG +PLPR YP    T  AS  K   +   PL    + +L S  G + K ++
Sbjct: 1   MASSVLSECGFRPLPRFYP-KHTTSFASNPKPTFKFNPPLKPP-SSLLNSRYGFYSKTRN 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNV+ PL       + + ER         FDPGA PPF LAD+RAAIP HCWVK+PW 
Sbjct: 61  WALNVATPLTTLQSPSEEDTER---------FDPGAPPPFNLADIRAAIPKHCWVKNPWM 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDV +V GLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSV GHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY++L+K T+  RFT
Sbjct: 181 NSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMFRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYP YLWNRSPGK+GSH+ P SDLF+P E+ D++TSTACW AM ALL CL+ VM
Sbjct: 241 LPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+EWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSM 420
           NNIHHDIGTHVIHHLFPQIPHYHL+EATEAAKPVLGKYYREPK S PLPLHLLG L+KSM
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPKNSGPLPLHLLGSLIKSM 420

Query: 421 RKDHYVSDSGDIVYYQTDPNLS 440
           ++DH+VSD+GD+VYY+ DP L+
Sbjct: 421 KQDHFVSDTGDVVYYEADPKLN 431

BLAST of MS017184 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 674.9 bits (1740), Expect = 4.5e-194
Identity = 319/453 (70.42%), Postives = 359/453 (79.25%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPS----------RTGLASKAFMEIRQPLSASRAHILRSSL 60
           MA  VLSECG++PLPRIY  P           R  L+S ++     PLS      L S  
Sbjct: 1   MANLVLSECGIRPLPRIYTTPRSNFLSNNNKFRPSLSSSSYKTSSSPLSFG----LNSRD 60

Query: 61  GLWKGKSWGLNVSAPLRV-----ASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAA 120
           G    ++W LNVS PL       + ++EDN++           FDPGA PPF LAD+RAA
Sbjct: 61  GF--TRNWALNVSTPLTTPIFEESPLEEDNKQR----------FDPGAPPPFNLADIRAA 120

Query: 121 IPNHCWVKDPWKSMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHD 180
           IP HCWVK+PWKS+SYVVRDV +V  LAA AAY NNW+VWPLYW AQGTMFWALFVLGHD
Sbjct: 121 IPKHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLYWLAQGTMFWALFVLGHD 180

Query: 181 CGHGSFSNNPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYR 240
           CGHGSFSN+P LNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKIY 
Sbjct: 181 CGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYN 240

Query: 241 SLDKATKTLRFTLPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVA 300
           +LDK T+  RFTLP  MLAYP YLW RSPGKKGSH+ P SDLF+P ER D++TSTACW A
Sbjct: 241 TLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERKDVLTSTACWTA 300

Query: 301 MVALLGCLSVVMGPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRG 360
           M ALL CL+  +GP+Q+LKLYGIPY + VMWLD VTYLHHHGH+DKLPWYRG+EWSYLRG
Sbjct: 301 MAALLVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRG 360

Query: 361 GLTTLDRDYGWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPL 420
           GLTTLDRDYG INNIHHDIGTHVIHHLFPQIPHYHL+EATEAAKPVLGKYYREP KS PL
Sbjct: 361 GLTTLDRDYGLINNIHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYREPDKSGPL 420

Query: 421 PLHLLGVLVKSMRKDHYVSDSGDIVYYQTDPNL 439
           PLHLL +L KS+++DHYVSD G++VYY+ DPNL
Sbjct: 421 PLHLLEILAKSIKEDHYVSDEGEVVYYKADPNL 437

BLAST of MS017184 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 606.7 bits (1563), Expect = 1.5e-173
Identity = 281/389 (72.24%), Postives = 315/389 (80.98%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPLPSRTGLAS--KAFMEIRQPLSASRAHILRSSLGLW-KGKS 60
           MA  VLSECG +PLPR YP    T  AS  K   +   PL    + +L S  G + K ++
Sbjct: 1   MASSVLSECGFRPLPRFYP-KHTTSFASNPKPTFKFNPPLKPP-SSLLNSRYGFYSKTRN 60

Query: 61  WGLNVSAPLRVASVDEDNERERVDVVGDDGGFDPGAAPPFRLADVRAAIPNHCWVKDPWK 120
           W LNV+ PL       + + ER         FDPGA PPF LAD+RAAIP HCWVK+PW 
Sbjct: 61  WALNVATPLTTLQSPSEEDTER---------FDPGAPPPFNLADIRAAIPKHCWVKNPWM 120

Query: 121 SMSYVVRDVVVVLGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNL 180
           SMSYVVRDV +V GLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFSN+P L
Sbjct: 121 SMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPRL 180

Query: 181 NSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFT 240
           NSV GHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY++L+K T+  RFT
Sbjct: 181 NSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMFRFT 240

Query: 241 LPFPMLAYPVYLWNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVM 300
           LPFPMLAYP YLWNRSPGK+GSH+ P SDLF+P E+ D++TSTACW AM ALL CL+ VM
Sbjct: 241 LPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVM 300

Query: 301 GPLQLLKLYGIPYLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWI 360
           GP+Q+LKLYGIPY +FVMWLD VTYLHHHGH+DKLPWYRG+EWSYLRGGLTTLDRDYGWI
Sbjct: 301 GPIQMLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWI 360

Query: 361 NNIHHDIGTHVIHHLFPQIPHYHLIEATE 387
           NNIHHDIGTHVIHHLFPQIPHYHL+EA +
Sbjct: 361 NNIHHDIGTHVIHHLFPQIPHYHLVEAKQ 378

BLAST of MS017184 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 568.5 bits (1464), Expect = 4.5e-162
Identity = 257/369 (69.65%), Postives = 303/369 (82.11%), Query Frame = 0

Query: 78  ERVDVVGDDGG--------FDPGAAPPFRLADVRAAIPNHCWVKDPWKSMSYVVRDVVVV 137
           +R +V GD G         FDP A PPF++ D+RAAIP HCWVK P +SMSYVVRD++ V
Sbjct: 7   QRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAV 66

Query: 138 LGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNLNSVVGHLLHSSI 197
             LA  A Y ++W +WPLYW AQGT+FWA+FVLGHDCGHGSFS+ P LNSVVGH+LHS I
Sbjct: 67  AALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFI 126

Query: 198 LVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFTLPFPMLAYPVYL 257
           LVPYHGWRISHRTHHQNHGHVENDESW PL E++Y+ L  +T+ LR+T+P PMLAYP+YL
Sbjct: 127 LVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPMLAYPLYL 186

Query: 258 WNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVMGPLQLLKLYGIP 317
             RSPGK+GSHF+P S LF PSER  I TST CW  M   L  LS V GPL +LK+YG+P
Sbjct: 187 CYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGVP 246

Query: 318 YLLFVMWLDVVTYLHHHGHDDKLPWYRGEEWSYLRGGLTTLDRDYGWINNIHHDIGTHVI 377
           Y++FVMWLD VTYLHHHGHD+KLPWYRG+EWSYLRGGLTT+DRDYG  NNIHHDIGTHVI
Sbjct: 247 YIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDIGTHVI 306

Query: 378 HHLFPQIPHYHLIEATEAAKPVLGKYYREPKKSLPLPLHLLGVLVKSMRKDHYVSDSGDI 437
           HHLFPQIPHYHL++AT+AAK VLG+YYREPK S  +P+HL+  LV S++KDHYVSD+GDI
Sbjct: 307 HHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVSDTGDI 366

Query: 438 VYYQTDPNL 439
           V+Y+TDP+L
Sbjct: 367 VFYETDPDL 375

BLAST of MS017184 vs. TAIR 10
Match: AT2G29980.2 (fatty acid desaturase 3 )

HSP 1 Score: 398.3 bits (1022), Expect = 8.1e-111
Identity = 180/269 (66.91%), Postives = 212/269 (78.81%), Query Frame = 0

Query: 78  ERVDVVGDDGG--------FDPGAAPPFRLADVRAAIPNHCWVKDPWKSMSYVVRDVVVV 137
           +R +V GD G         FDP A PPF++ D+RAAIP HCWVK P +SMSYVVRD++ V
Sbjct: 7   QRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAV 66

Query: 138 LGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNNPNLNSVVGHLLHSSI 197
             LA  A Y ++W +WPLYW AQGT+FWA+FVLGHDCGHGSFS+ P LNSVVGH+LHS I
Sbjct: 67  AALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFI 126

Query: 198 LVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATKTLRFTLPFPMLAYPVYL 257
           LVPYHGWRISHRTHHQNHGHVENDESW PL E++Y+ L  +T+ LR+T+P PMLAYP+YL
Sbjct: 127 LVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPMLAYPLYL 186

Query: 258 WNRSPGKKGSHFDPSSDLFVPSERNDIITSTACWVAMVALLGCLSVVMGPLQLLKLYGIP 317
             RSPGK+GSHF+P S LF PSER  I TST CW  M   L  LS V GPL +LK+YG+P
Sbjct: 187 CYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGVP 246

Query: 318 YLLFVMWLDVVTYLHHHGHDDKLPWYRGE 339
           Y++FVMWLD VTYLHHHGHD+KLPWYRG+
Sbjct: 247 YIIFVMWLDAVTYLHHHGHDEKLPWYRGK 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892287.11.9e-24289.44omega-3 fatty acid desaturase, chloroplastic [Benincasa hispida][more]
XP_008465212.11.6e-24189.21PREDICTED: omega-3 fatty acid desaturase, chloroplastic [Cucumis melo] >KAA00528... [more]
NP_001274404.13.9e-24088.09omega-3 fatty acid desaturase [Cucumis sativus] >ACE80931.1 omega-3 fatty acid d... [more]
XP_022995701.12.6e-23687.56omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita maxima][more]
XP_023547843.13.4e-23687.33omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P486206.5e-20675.85Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum OX=4182 GN=FAD7 ... [more]
P486194.2e-20575.44Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis OX=3988 GN=FAD7... [more]
P486225.3e-20073.08Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloropla... [more]
P486214.2e-19770.86Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 GN=FAD7 PE=2... [more]
Q56VS46.1e-19671.27sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Helianthus ann... [more]
Match NameE-valueIdentityDescription
A0A5A7UH667.6e-24289.21Omega-3 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CNS07.6e-24289.21omega-3 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350... [more]
B3VA191.9e-24088.09Omega-3 fatty acid desaturase OS=Cucumis sativus OX=3659 GN=fad7 PE=2 SV=1[more]
A0A6J1K2M71.3e-23687.56omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1FSG48.2e-23687.10omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT5G05580.13.8e-20173.08fatty acid desaturase 8 [more]
AT3G11170.14.5e-19470.42fatty acid desaturase 7 [more]
AT5G05580.21.5e-17372.24fatty acid desaturase 8 [more]
AT2G29980.14.5e-16269.65fatty acid desaturase 3 [more]
AT2G29980.28.1e-11166.91fatty acid desaturase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 142..396
e-value: 1.0E-32
score: 114.0
IPR021863Fatty acid desaturase, N-terminalPFAMPF11960DUF3474coord: 1..134
e-value: 1.8E-50
score: 170.7
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 42..438
NoneNo IPR availablePANTHERPTHR32100:SF52TEMPERATURE-SENSITIVE SN-2 ACYL-LIPID OMEGA-3 DESATURASE (FERREDOXIN), CHLOROPLASTICcoord: 42..438
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 113..381
e-value: 4.22311E-76
score: 234.812

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017184.1MS017184.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water