MS017088 (gene) Bitter gourd (TR) v1

Overview
NameMS017088
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsyntaxin-related protein KNOLLE
Locationscaffold197: 1330899 .. 1332037 (-)
RNA-Seq ExpressionMS017088
SyntenyMS017088
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGATCTGATGACCAAATCCTTCACCAGCTACGTGGATCTGAAGAAGGCTGCCATGAAAGACATCGACCTCGAGGCCGGTTTAGAAATGGCGTCCACTGACAGCGGCGGTGACATGGTTCTCTTCCTCGAAGAGGCCGAGAAGGTGAAATCGGAGATGGGTTCGATTCGAGAGATCCTTGGAAAGCTCCAGCAGGCCAATGAGGAGACCAAATCGGCTCACAAACCGGAAACTTTGAAATCTCTCCGCCACACCATTAACGTCGACATCGTCACCGTCCTGAAGAAGGCGAGATCGATCCGATCCCAGCTCGAGGAAATGGACCGCACCAATGCTGCCAAGAAGAGGATCTCTGGCAGCAAAGAAGGATCCGCCATTTACAGGTACTAAACCGATAAATTACAGTAAATTTGATTAGTTCTGGACTTAGATGGAAACTTTTTACTCTGCTCTGATCGTAAATTTAATCTCCATCCACACGCAAACTGAACAAAATAGGATGCAAATCATTTGAGTCATTTTAGCGTAGTTCTAGATTTAAATTGAAATCTCTAATTCTACTCTGGATGGGTTACTCTCGTCCGTATGTTCAACAAACCAGGACGCGGATGGCGGTGACAAACGGGCTGCGGAAGAAGCTAAAGGAGCTAATGATGGAGTTCCAGAGCCTGAGGCAACGGATGATGACGGAGTACAAGGAGACGGTGGGGCGGCGGTACTTCACGGTGACGGGGGAGGAGGCGGAGGAGGAGGTGATAGAGAAGATAATAGAGAACGGCGGGGAGGAGTTTCTGGGGAGGGCGATAGAGGAGCACGGGCGGGGGAAGGTGGCGGAGACGGTGGTGGAGATACAGGACCGGCACGGGGCGGCGAAGGAGATAGAGAGGAGCTTGCTGGAGCTGCACCAGGTGTTCTTGGACATGGCAGTGATGGTGGAAGCACAGGGGGAGAAGATGGATGACATTGAACACCATGTCATCAATGCTTCACAGTATGTGACAGATGGGACTAAGGATTTGAAGAATGCTAAGGATTTGCAGAGGAGCAGTAGGAAATGGATGTGTTTGGGGATTTTGCTTCTCATTCTCATTGTTTTTGTTGTTGTTATTCCCATTGCTGTTAGTTTTGGGAGTTCT

mRNA sequence

ATGAACGATCTGATGACCAAATCCTTCACCAGCTACGTGGATCTGAAGAAGGCTGCCATGAAAGACATCGACCTCGAGGCCGGTTTAGAAATGGCGTCCACTGACAGCGGCGGTGACATGGTTCTCTTCCTCGAAGAGGCCGAGAAGGTGAAATCGGAGATGGGTTCGATTCGAGAGATCCTTGGAAAGCTCCAGCAGGCCAATGAGGAGACCAAATCGGCTCACAAACCGGAAACTTTGAAATCTCTCCGCCACACCATTAACGTCGACATCGTCACCGTCCTGAAGAAGGCGAGATCGATCCGATCCCAGCTCGAGGAAATGGACCGCACCAATGCTGCCAAGAAGAGGATCTCTGGCAGCAAAGAAGGATCCGCCATTTACAGGACGCGGATGGCGGTGACAAACGGGCTGCGGAAGAAGCTAAAGGAGCTAATGATGGAGTTCCAGAGCCTGAGGCAACGGATGATGACGGAGTACAAGGAGACGGTGGGGCGGCGGTACTTCACGGTGACGGGGGAGGAGGCGGAGGAGGAGGTGATAGAGAAGATAATAGAGAACGGCGGGGAGGAGTTTCTGGGGAGGGCGATAGAGGAGCACGGGCGGGGGAAGGTGGCGGAGACGGTGGTGGAGATACAGGACCGGCACGGGGCGGCGAAGGAGATAGAGAGGAGCTTGCTGGAGCTGCACCAGGTGTTCTTGGACATGGCAGTGATGGTGGAAGCACAGGGGGAGAAGATGGATGACATTGAACACCATGTCATCAATGCTTCACAGTATGTGACAGATGGGACTAAGGATTTGAAGAATGCTAAGGATTTGCAGAGGAGCAGTAGGAAATGGATGTGTTTGGGGATTTTGCTTCTCATTCTCATTGTTTTTGTTGTTGTTATTCCCATTGCTGTTAGTTTTGGGAGTTCT

Coding sequence (CDS)

ATGAACGATCTGATGACCAAATCCTTCACCAGCTACGTGGATCTGAAGAAGGCTGCCATGAAAGACATCGACCTCGAGGCCGGTTTAGAAATGGCGTCCACTGACAGCGGCGGTGACATGGTTCTCTTCCTCGAAGAGGCCGAGAAGGTGAAATCGGAGATGGGTTCGATTCGAGAGATCCTTGGAAAGCTCCAGCAGGCCAATGAGGAGACCAAATCGGCTCACAAACCGGAAACTTTGAAATCTCTCCGCCACACCATTAACGTCGACATCGTCACCGTCCTGAAGAAGGCGAGATCGATCCGATCCCAGCTCGAGGAAATGGACCGCACCAATGCTGCCAAGAAGAGGATCTCTGGCAGCAAAGAAGGATCCGCCATTTACAGGACGCGGATGGCGGTGACAAACGGGCTGCGGAAGAAGCTAAAGGAGCTAATGATGGAGTTCCAGAGCCTGAGGCAACGGATGATGACGGAGTACAAGGAGACGGTGGGGCGGCGGTACTTCACGGTGACGGGGGAGGAGGCGGAGGAGGAGGTGATAGAGAAGATAATAGAGAACGGCGGGGAGGAGTTTCTGGGGAGGGCGATAGAGGAGCACGGGCGGGGGAAGGTGGCGGAGACGGTGGTGGAGATACAGGACCGGCACGGGGCGGCGAAGGAGATAGAGAGGAGCTTGCTGGAGCTGCACCAGGTGTTCTTGGACATGGCAGTGATGGTGGAAGCACAGGGGGAGAAGATGGATGACATTGAACACCATGTCATCAATGCTTCACAGTATGTGACAGATGGGACTAAGGATTTGAAGAATGCTAAGGATTTGCAGAGGAGCAGTAGGAAATGGATGTGTTTGGGGATTTTGCTTCTCATTCTCATTGTTTTTGTTGTTGTTATTCCCATTGCTGTTAGTTTTGGGAGTTCT

Protein sequence

MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEVIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPIAVSFGSS
Homology
BLAST of MS017088 vs. NCBI nr
Match: XP_022156189.1 (syntaxin-related protein KNOLLE [Momordica charantia])

HSP 1 Score: 568.5 bits (1464), Expect = 3.4e-158
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
           LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKR+SG
Sbjct: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRLSG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
           SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV
Sbjct: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180

Query: 181 IEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMV 240
           IEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMV
Sbjct: 181 IEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMV 240

Query: 241 EAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPI 300
           EAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPI
Sbjct: 241 EAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPI 300

Query: 301 AVSFGSS 308
           AVSFGSS
Sbjct: 301 AVSFGSS 307

BLAST of MS017088 vs. NCBI nr
Match: XP_038881435.1 (syntaxin-related protein KNOLLE [Benincasa hispida])

HSP 1 Score: 510.4 bits (1313), Expect = 1.1e-140
Identity = 275/309 (89.00%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMAS--TDSGGDMVLFLEEAEKVKSEMGSIR 60
           MNDLMTKSFTSYVDLKKAAMKD+DLE+GLEM S  T+  GDM LFLEEAEKVK+EMGSIR
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDLDLESGLEMPSSITNDNGDMGLFLEEAEKVKTEMGSIR 60

Query: 61  EILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRI 120
           EIL KLQQANEETKSAHKPE LKSLR+TIN DI+TVLKKARSIRSQLEEMDR NAAKKR+
Sbjct: 61  EILVKLQQANEETKSAHKPEILKSLRNTINADIITVLKKARSIRSQLEEMDRANAAKKRL 120

Query: 121 SGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEE 180
           SGSKEG+AIYRTR+AVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGE  EE
Sbjct: 121 SGSKEGTAIYRTRIAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEHPEE 180

Query: 181 EVIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAV 240
           EVIEKII NGGEEFLG+AI+EHGRGKVAETVVEIQDRHGAAKEIE+SLLELHQVFLDMAV
Sbjct: 181 EVIEKIISNGGEEFLGKAIKEHGRGKVAETVVEIQDRHGAAKEIEKSLLELHQVFLDMAV 240

Query: 241 MVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVI 300
           MVEAQGEKMDDIEHHV+NASQYV DGTKDLK AKDLQR+SRK+MC+GILLL+LI+ VVVI
Sbjct: 241 MVEAQGEKMDDIEHHVMNASQYVRDGTKDLKTAKDLQRNSRKYMCVGILLLLLIILVVVI 300

Query: 301 PIAVSFGSS 308
           PIAVSFGSS
Sbjct: 301 PIAVSFGSS 309

BLAST of MS017088 vs. NCBI nr
Match: KAG7027497.1 (Syntaxin-related protein KNOLLE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 509.2 bits (1310), Expect = 2.4e-140
Identity = 275/308 (89.29%), Postives = 291/308 (94.48%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDS-GGDMVLFLEEAEKVKSEMGSIRE 60
           MNDLMT+SFTSYVDLKKAAMKDIDLEAGLE AS+D+  GDM LFLEEAEKVK EMGSIRE
Sbjct: 1   MNDLMTQSFTSYVDLKKAAMKDIDLEAGLETASSDTDNGDMGLFLEEAEKVKLEMGSIRE 60

Query: 61  ILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRIS 120
           ILGKLQQANEETKSAHKPETLKSLR TIN DIVTVLKKARSIRS+LEEMDR N AKKR+S
Sbjct: 61  ILGKLQQANEETKSAHKPETLKSLRQTINGDIVTVLKKARSIRSKLEEMDRANDAKKRLS 120

Query: 121 GSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEE 180
            SKEG+AIYRTR+AVTNGLRKKLKELMMEFQ+LRQRMMTEYKETV RRYFTVTGE  EEE
Sbjct: 121 ASKEGTAIYRTRIAVTNGLRKKLKELMMEFQTLRQRMMTEYKETVERRYFTVTGEHPEEE 180

Query: 181 VIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           VIEKII NGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM
Sbjct: 181 VIEKIISNGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQGEKMDDIEHHV+NASQYV DG+KDLK+AKDLQRS+RKWMC G+LLL++I+ VVVIP
Sbjct: 241 VEAQGEKMDDIEHHVMNASQYVRDGSKDLKSAKDLQRSNRKWMCFGVLLLLIIILVVVIP 300

Query: 301 IAVSFGSS 308
           IAVSFGSS
Sbjct: 301 IAVSFGSS 308

BLAST of MS017088 vs. NCBI nr
Match: XP_004147530.1 (syntaxin-related protein KNOLLE [Cucumis sativus] >KGN53948.1 hypothetical protein Csa_021581 [Cucumis sativus])

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-139
Identity = 276/309 (89.32%), Postives = 291/309 (94.17%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSG--GDMVLFLEEAEKVKSEMGSIR 60
           MNDLMTKSFTSYVDLKKAAMKD+DLEAGLE AS+ +G  GDM LFLEEAEKVK+EMGSIR
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDLDLEAGLEKASSVTGDNGDMGLFLEEAEKVKTEMGSIR 60

Query: 61  EILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRI 120
           EIL KLQQANEETKSAHKPETLK LR+ INVDIVTVLKKARSIRSQLEEMDR NAAKKR+
Sbjct: 61  EILVKLQQANEETKSAHKPETLKLLRNAINVDIVTVLKKARSIRSQLEEMDRANAAKKRL 120

Query: 121 SGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEE 180
           SGSKEG+AIYRTR+AVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGE  EE
Sbjct: 121 SGSKEGTAIYRTRIAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEHPEE 180

Query: 181 EVIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAV 240
           EVIEKII NGGEEFL RAIEEHGRGKVAETVVEIQDRHGAAKEIE+SLLELHQVFLDMAV
Sbjct: 181 EVIEKIISNGGEEFLARAIEEHGRGKVAETVVEIQDRHGAAKEIEKSLLELHQVFLDMAV 240

Query: 241 MVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVI 300
           MVEAQGEKMDDIEHHV+NASQYV DGTKDLK AKDLQR+SRK +C GILLL++I+ VVVI
Sbjct: 241 MVEAQGEKMDDIEHHVMNASQYVIDGTKDLKTAKDLQRNSRKCLCFGILLLLVIILVVVI 300

Query: 301 PIAVSFGSS 308
           PIAVSFGSS
Sbjct: 301 PIAVSFGSS 309

BLAST of MS017088 vs. NCBI nr
Match: XP_022925127.1 (syntaxin-related protein KNOLLE-like [Cucurbita moschata])

HSP 1 Score: 505.8 bits (1301), Expect = 2.7e-139
Identity = 273/308 (88.64%), Postives = 290/308 (94.16%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDS-GGDMVLFLEEAEKVKSEMGSIRE 60
           MNDLMT+SFTSYVDLKKAAMKDIDLEAGLE  S+D+  GDM LFLEEAEKVK EMGSIRE
Sbjct: 1   MNDLMTQSFTSYVDLKKAAMKDIDLEAGLETVSSDTDNGDMGLFLEEAEKVKLEMGSIRE 60

Query: 61  ILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRIS 120
           ILGKLQQANEETKSAHKPETLKSLR TIN DIVTVLKKARSIRS+LEEMDR N AKKR+S
Sbjct: 61  ILGKLQQANEETKSAHKPETLKSLRQTINGDIVTVLKKARSIRSKLEEMDRANDAKKRLS 120

Query: 121 GSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEE 180
            SKEG+AIYRTR+AVTNGLRKKLKELMMEFQ+LRQRMMTE+KETV RRYFTVTGE  EEE
Sbjct: 121 ASKEGTAIYRTRIAVTNGLRKKLKELMMEFQTLRQRMMTEHKETVERRYFTVTGEHPEEE 180

Query: 181 VIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           VIEKII NGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM
Sbjct: 181 VIEKIISNGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQGEKMDDIEHHV+NASQYV DG+KDLK+AKDLQRS+RKWMC G+LLL++I+ VVVIP
Sbjct: 241 VEAQGEKMDDIEHHVMNASQYVRDGSKDLKSAKDLQRSNRKWMCFGVLLLLIIILVVVIP 300

Query: 301 IAVSFGSS 308
           IAVSFGSS
Sbjct: 301 IAVSFGSS 308

BLAST of MS017088 vs. ExPASy Swiss-Prot
Match: Q42374 (Syntaxin-related protein KNOLLE OS=Arabidopsis thaliana OX=3702 GN=KN PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.5e-103
Identity = 212/316 (67.09%), Postives = 258/316 (81.65%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAG----LEMASTDS---GGDMVLFLEEAEKVKSE 60
           MNDLMTKSF SYVDLKKAAMK  D+EAG    LEMAST +     ++  FLEEAE VK+E
Sbjct: 1   MNDLMTKSFMSYVDLKKAAMK--DMEAGPDFDLEMASTKADKMDENLSSFLEEAEYVKAE 60

Query: 61  MGSIREILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNA 120
           MG I E L +++Q +EE+K  HK E++KSLR+ I+ +IV+ L+KA+SI+S+LEEMD+ N 
Sbjct: 61  MGLISETLARIEQYHEESKGVHKAESVKSLRNKISNEIVSGLRKAKSIKSKLEEMDKANK 120

Query: 121 AKKRISGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTG 180
             KR+S    G+ +YR+R AVTNGLRKKLKE+MMEFQ LRQ+MM+EYKETV RRYFTVTG
Sbjct: 121 EIKRLS----GTPVYRSRTAVTNGLRKKLKEVMMEFQGLRQKMMSEYKETVERRYFTVTG 180

Query: 181 EEAEEEVIEKIIEN--GGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQ 240
           E A +E+IEKII +  GGEEFL RAI+EHG+GKV ETVVEIQDR+ AAKEIE+SLLELHQ
Sbjct: 181 EHANDEMIEKIITDNAGGEEFLTRAIQEHGKGKVLETVVEIQDRYDAAKEIEKSLLELHQ 240

Query: 241 VFLDMAVMVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLIL 300
           VFLDMAVMVE+QGE+MD+IEHHVINAS YV DG  +LK AK  QR+SRKWMC+GI++L+L
Sbjct: 241 VFLDMAVMVESQGEQMDEIEHHVINASHYVADGANELKTAKSHQRNSRKWMCIGIIVLLL 300

Query: 301 IVFVVVIPIAVSFGSS 308
           I+ +VVIPI  SF SS
Sbjct: 301 IILIVVIPIITSFSSS 310

BLAST of MS017088 vs. ExPASy Swiss-Prot
Match: O64791 (Syntaxin-124 OS=Arabidopsis thaliana OX=3702 GN=SYP124 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.1e-69
Identity = 144/300 (48.00%), Postives = 205/300 (68.33%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDL + SF  Y DLK+ A  D D+E+G E  + D       F E+ E VK  M  +  +
Sbjct: 1   MNDLFSSSFKKYTDLKQQAQMD-DIESGKETMNLDK------FFEDVENVKDNMKGVETL 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
              LQ +NEE K+ H  + +K LR  ++ D+  VLK+ + I+ +LE +++ NA  + +SG
Sbjct: 61  YKSLQDSNEECKTVHNAKKVKELRAKMDGDVAQVLKRVKMIKQKLEALEKANANSRNVSG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
              GS+  RTR +V +GL KKLK+LM  FQ LR RM  EYKETV RRYFT+TGE+A+E+ 
Sbjct: 121 CGPGSSTDRTRTSVVSGLGKKLKDLMDSFQGLRARMNAEYKETVERRYFTITGEQADEQT 180

Query: 181 IEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           IE +I +G  E FL +AI+E GRG++ +T+ EIQ+RH A KEIE++L+ELHQVFLDMA +
Sbjct: 181 IENLISSGESENFLQKAIQEQGRGQILDTISEIQERHDAVKEIEKNLIELHQVFLDMAAL 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VE+QG++++DIE HV  AS +V  GT  L++A++ Q+SSRKW C  ILL I++  +++IP
Sbjct: 241 VESQGQQLNDIESHVSKASSFVRRGTDQLQDAREYQKSSRKWTCYAILLFIVVFALLLIP 293

BLAST of MS017088 vs. ExPASy Swiss-Prot
Match: Q9ZQZ8 (Syntaxin-123 OS=Arabidopsis thaliana OX=3702 GN=SYP123 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.8e-68
Identity = 147/301 (48.84%), Postives = 211/301 (70.10%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDL++ SF  Y DL      D D+E+  +  S DS G++  F    E VK +M ++ EI
Sbjct: 1   MNDLISSSFKRYTDLNHQVQLD-DIES--QNVSLDS-GNLDEFFGYVESVKEDMKAVDEI 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
             +LQ ANEE+K+ H  + +K LR  ++  +  VLK+ + I+++L  ++++NAA+++++G
Sbjct: 61  HKRLQDANEESKTVHDSKAVKKLRARMDSSVTEVLKRVKMIKTKLVALEKSNAAQRKVAG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
              GS+  RTR +V +GL KKLK++M +FQ LR +M TEYKETV RRYFTVTG++A+EE 
Sbjct: 121 CGPGSSADRTRTSVVSGLGKKLKDMMDDFQRLRTKMATEYKETVERRYFTVTGQKADEET 180

Query: 181 IEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           +EK+I +G  E FL +AI+E GRG+V +T+ EIQ+RH   KEIERSLLELHQVFLDMA +
Sbjct: 181 VEKLISSGESERFLQKAIQEQGRGQVMDTLSEIQERHDTVKEIERSLLELHQVFLDMAAL 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQG  ++DIE +V  AS +V  GT  L  AK LQR++RKW C+  +L I++V V++ P
Sbjct: 241 VEAQGNMLNDIESNVSKASSFVMRGTDQLHGAKVLQRNNRKWACIATILAIVVVIVILFP 297

BLAST of MS017088 vs. ExPASy Swiss-Prot
Match: Q9SXB0 (Syntaxin-125 OS=Arabidopsis thaliana OX=3702 GN=SYP125 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.6e-65
Identity = 141/301 (46.84%), Postives = 203/301 (67.44%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDL + SF      KK   +  D+EAG E  + D       F E+ E VK +M  +  +
Sbjct: 1   MNDLFSNSF------KKNQAQLGDVEAGQETMNLDK------FFEDVENVKDDMKGVEAL 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
             KLQ +NEE K+ H  + +K LR  ++ D+  VLK+ + I+ +LE +++ NA  + + G
Sbjct: 61  YKKLQDSNEECKTVHNAKKVKELRAKMDGDVAMVLKRVKIIKQKLEALEKANANSRNVPG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
              GS+  RTR +V +GL KKLK+LM  FQ LR RM  EYKETV RRYFT+TGE+A+E+ 
Sbjct: 121 CGPGSSTDRTRSSVVSGLGKKLKDLMDSFQGLRARMNNEYKETVERRYFTITGEKADEQT 180

Query: 181 IEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           I+ +I +G  E FL +AI+E GRG++ +T+ EIQ+RH A KEIE++LLELHQVFLDMA +
Sbjct: 181 IDNLIASGESENFLQKAIQEQGRGQILDTISEIQERHDAVKEIEKNLLELHQVFLDMAAL 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQG+++++IE HV  AS +V  GT  L++A++ Q+SSRKW C  I+L I+I  +++IP
Sbjct: 241 VEAQGQQLNNIESHVAKASSFVRRGTDQLQDAREYQKSSRKWTCYAIILFIVIFILLLIP 289

BLAST of MS017088 vs. ExPASy Swiss-Prot
Match: Q9ZSD4 (Syntaxin-121 OS=Arabidopsis thaliana OX=3702 GN=SYP121 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-60
Identity = 133/312 (42.63%), Postives = 205/312 (65.71%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEA-GLEMAS---TDSGGDMVLFLEEAEKVKSEMGS 60
           MNDL + SF+ +   + +  +D+     G++MA+   +  G ++  F E+ E VK E+  
Sbjct: 1   MNDLFSSSFSRFRSGEPSPRRDVAGGGDGVQMANPAGSTGGVNLDKFFEDVESVKEELKE 60

Query: 61  IREILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKK 120
           +  +   L   +E++K+ H  + +K LR  ++ D+   LKKA+ I+ +LE +DR NAA +
Sbjct: 61  LDRLNETLSSCHEQSKTLHNAKAVKDLRSKMDGDVGVALKKAKMIKVKLEALDRANAANR 120

Query: 121 RISGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEA 180
            + G   GS+  RTR +V NGLRKKL + M  F  LR+ + +EY+ETV RRYFTVTGE  
Sbjct: 121 SLPGCGPGSSSDRTRTSVLNGLRKKLMDSMDSFNRLRELISSEYRETVQRRYFTVTGENP 180

Query: 181 EEEVIEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLD 240
           +E  ++++I  G  E FL +AI+E GRG+V +T+ EIQ+RH A K+IE++L ELHQVFLD
Sbjct: 181 DERTLDRLISTGESERFLQKAIQEQGRGRVLDTINEIQERHDAVKDIEKNLRELHQVFLD 240

Query: 241 MAVMVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFV 300
           MAV+VE QG ++DDIE HV  AS ++  GT  L+ A+  Q+++RKW C+ I++LI+I+ V
Sbjct: 241 MAVLVEHQGAQLDDIESHVGRASSFIRGGTDQLQTARVYQKNTRKWTCIAIIILIIIITV 300

Query: 301 VVIPIAVSFGSS 308
           VV+ +   + +S
Sbjct: 301 VVLAVLKPWNNS 312

BLAST of MS017088 vs. ExPASy TrEMBL
Match: A0A6J1DSL9 (syntaxin-related protein KNOLLE OS=Momordica charantia OX=3673 GN=LOC111023138 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.6e-158
Identity = 306/307 (99.67%), Postives = 307/307 (100.00%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
           LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKR+SG
Sbjct: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRLSG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
           SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV
Sbjct: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180

Query: 181 IEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMV 240
           IEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMV
Sbjct: 181 IEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVMV 240

Query: 241 EAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPI 300
           EAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPI
Sbjct: 241 EAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIPI 300

Query: 301 AVSFGSS 308
           AVSFGSS
Sbjct: 301 AVSFGSS 307

BLAST of MS017088 vs. ExPASy TrEMBL
Match: A0A0A0KWP3 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G192200 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.9e-140
Identity = 276/309 (89.32%), Postives = 291/309 (94.17%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSG--GDMVLFLEEAEKVKSEMGSIR 60
           MNDLMTKSFTSYVDLKKAAMKD+DLEAGLE AS+ +G  GDM LFLEEAEKVK+EMGSIR
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDLDLEAGLEKASSVTGDNGDMGLFLEEAEKVKTEMGSIR 60

Query: 61  EILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRI 120
           EIL KLQQANEETKSAHKPETLK LR+ INVDIVTVLKKARSIRSQLEEMDR NAAKKR+
Sbjct: 61  EILVKLQQANEETKSAHKPETLKLLRNAINVDIVTVLKKARSIRSQLEEMDRANAAKKRL 120

Query: 121 SGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEE 180
           SGSKEG+AIYRTR+AVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGE  EE
Sbjct: 121 SGSKEGTAIYRTRIAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEHPEE 180

Query: 181 EVIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAV 240
           EVIEKII NGGEEFL RAIEEHGRGKVAETVVEIQDRHGAAKEIE+SLLELHQVFLDMAV
Sbjct: 181 EVIEKIISNGGEEFLARAIEEHGRGKVAETVVEIQDRHGAAKEIEKSLLELHQVFLDMAV 240

Query: 241 MVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVI 300
           MVEAQGEKMDDIEHHV+NASQYV DGTKDLK AKDLQR+SRK +C GILLL++I+ VVVI
Sbjct: 241 MVEAQGEKMDDIEHHVMNASQYVIDGTKDLKTAKDLQRNSRKCLCFGILLLLVIILVVVI 300

Query: 301 PIAVSFGSS 308
           PIAVSFGSS
Sbjct: 301 PIAVSFGSS 309

BLAST of MS017088 vs. ExPASy TrEMBL
Match: A0A5A7UY08 (Syntaxin-related protein KNOLLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00470 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 276/309 (89.32%), Postives = 289/309 (93.53%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSG--GDMVLFLEEAEKVKSEMGSIR 60
           MNDLMTKSFTSYVDLKKAAMKD+DLEAGLEMAS+ +G  GDM  FLEEAEKVK EMGSIR
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDLDLEAGLEMASSVNGDNGDMGQFLEEAEKVKMEMGSIR 60

Query: 61  EILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRI 120
           EIL KLQQANEETKSAHKPETLK LR  INVDIVTVLKKARSIRSQLEEMDR NAAKKR+
Sbjct: 61  EILVKLQQANEETKSAHKPETLKLLRDAINVDIVTVLKKARSIRSQLEEMDRANAAKKRL 120

Query: 121 SGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEE 180
           SGSKEGSAIYRTR+AVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGE  EE
Sbjct: 121 SGSKEGSAIYRTRIAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEHPEE 180

Query: 181 EVIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAV 240
           EVIEKII NGGEEFL RAI+EHGRGKVAETVVEIQDRHGAAKEIE+SLLELHQVFLDMAV
Sbjct: 181 EVIEKIISNGGEEFLARAIQEHGRGKVAETVVEIQDRHGAAKEIEKSLLELHQVFLDMAV 240

Query: 241 MVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVI 300
           MVEAQGEKMDDIEHHV+NASQYV DGTKDLK AKDLQR+SRK MC GILLL++++ VVVI
Sbjct: 241 MVEAQGEKMDDIEHHVMNASQYVRDGTKDLKTAKDLQRNSRKCMCFGILLLLVLILVVVI 300

Query: 301 PIAVSFGSS 308
           PIAVSFGSS
Sbjct: 301 PIAVSFGSS 309

BLAST of MS017088 vs. ExPASy TrEMBL
Match: A0A6J1EAY4 (syntaxin-related protein KNOLLE-like OS=Cucurbita moschata OX=3662 GN=LOC111432461 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 273/308 (88.64%), Postives = 290/308 (94.16%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDS-GGDMVLFLEEAEKVKSEMGSIRE 60
           MNDLMT+SFTSYVDLKKAAMKDIDLEAGLE  S+D+  GDM LFLEEAEKVK EMGSIRE
Sbjct: 1   MNDLMTQSFTSYVDLKKAAMKDIDLEAGLETVSSDTDNGDMGLFLEEAEKVKLEMGSIRE 60

Query: 61  ILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRIS 120
           ILGKLQQANEETKSAHKPETLKSLR TIN DIVTVLKKARSIRS+LEEMDR N AKKR+S
Sbjct: 61  ILGKLQQANEETKSAHKPETLKSLRQTINGDIVTVLKKARSIRSKLEEMDRANDAKKRLS 120

Query: 121 GSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEE 180
            SKEG+AIYRTR+AVTNGLRKKLKELMMEFQ+LRQRMMTE+KETV RRYFTVTGE  EEE
Sbjct: 121 ASKEGTAIYRTRIAVTNGLRKKLKELMMEFQTLRQRMMTEHKETVERRYFTVTGEHPEEE 180

Query: 181 VIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           VIEKII NGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM
Sbjct: 181 VIEKIISNGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQGEKMDDIEHHV+NASQYV DG+KDLK+AKDLQRS+RKWMC G+LLL++I+ VVVIP
Sbjct: 241 VEAQGEKMDDIEHHVMNASQYVRDGSKDLKSAKDLQRSNRKWMCFGVLLLLIIILVVVIP 300

Query: 301 IAVSFGSS 308
           IAVSFGSS
Sbjct: 301 IAVSFGSS 308

BLAST of MS017088 vs. ExPASy TrEMBL
Match: A0A1S3B445 (syntaxin-related protein KNOLLE OS=Cucumis melo OX=3656 GN=LOC103485972 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 276/309 (89.32%), Postives = 289/309 (93.53%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSG--GDMVLFLEEAEKVKSEMGSIR 60
           MNDLMTKSFTSYVDLKKAAMKD+DLEAGLEMAS+ +G  GDM  FLEEAEKVK EMGSIR
Sbjct: 1   MNDLMTKSFTSYVDLKKAAMKDLDLEAGLEMASSVNGDNGDMGQFLEEAEKVKMEMGSIR 60

Query: 61  EILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRI 120
           EIL KLQQANEETKSAHKPETLK LR  INVDIVTVLKKARSIRSQLEEMDR NAAKKR+
Sbjct: 61  EILVKLQQANEETKSAHKPETLKLLRDAINVDIVTVLKKARSIRSQLEEMDRANAAKKRL 120

Query: 121 SGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEE 180
           SGSKEGSAIYRTR+AVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGE  EE
Sbjct: 121 SGSKEGSAIYRTRIAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEHPEE 180

Query: 181 EVIEKIIENGGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAV 240
           EVIEKII NGGEEFL RAI+EHGRGKVAETVVEIQDRHGAAKEIE+SLLELHQVFLDMAV
Sbjct: 181 EVIEKIISNGGEEFLARAIQEHGRGKVAETVVEIQDRHGAAKEIEKSLLELHQVFLDMAV 240

Query: 241 MVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVI 300
           MVEAQGEKMDDIEHHV+NASQYV DGTKDLK AKDLQR+SRK MC GILLL++++ VVVI
Sbjct: 241 MVEAQGEKMDDIEHHVMNASQYVRDGTKDLKTAKDLQRNSRKCMCFGILLLLVLILVVVI 300

Query: 301 PIAVSFGSS 308
           PIAVSFGSS
Sbjct: 301 PIAVSFGSS 309

BLAST of MS017088 vs. TAIR 10
Match: AT1G08560.1 (syntaxin of plants 111 )

HSP 1 Score: 375.6 bits (963), Expect = 3.9e-104
Identity = 212/316 (67.09%), Postives = 258/316 (81.65%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAG----LEMASTDS---GGDMVLFLEEAEKVKSE 60
           MNDLMTKSF SYVDLKKAAMK  D+EAG    LEMAST +     ++  FLEEAE VK+E
Sbjct: 1   MNDLMTKSFMSYVDLKKAAMK--DMEAGPDFDLEMASTKADKMDENLSSFLEEAEYVKAE 60

Query: 61  MGSIREILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNA 120
           MG I E L +++Q +EE+K  HK E++KSLR+ I+ +IV+ L+KA+SI+S+LEEMD+ N 
Sbjct: 61  MGLISETLARIEQYHEESKGVHKAESVKSLRNKISNEIVSGLRKAKSIKSKLEEMDKANK 120

Query: 121 AKKRISGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTG 180
             KR+S    G+ +YR+R AVTNGLRKKLKE+MMEFQ LRQ+MM+EYKETV RRYFTVTG
Sbjct: 121 EIKRLS----GTPVYRSRTAVTNGLRKKLKEVMMEFQGLRQKMMSEYKETVERRYFTVTG 180

Query: 181 EEAEEEVIEKIIEN--GGEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQ 240
           E A +E+IEKII +  GGEEFL RAI+EHG+GKV ETVVEIQDR+ AAKEIE+SLLELHQ
Sbjct: 181 EHANDEMIEKIITDNAGGEEFLTRAIQEHGKGKVLETVVEIQDRYDAAKEIEKSLLELHQ 240

Query: 241 VFLDMAVMVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLIL 300
           VFLDMAVMVE+QGE+MD+IEHHVINAS YV DG  +LK AK  QR+SRKWMC+GI++L+L
Sbjct: 241 VFLDMAVMVESQGEQMDEIEHHVINASHYVADGANELKTAKSHQRNSRKWMCIGIIVLLL 300

Query: 301 IVFVVVIPIAVSFGSS 308
           I+ +VVIPI  SF SS
Sbjct: 301 IILIVVIPIITSFSSS 310

BLAST of MS017088 vs. TAIR 10
Match: AT1G61290.1 (syntaxin of plants 124 )

HSP 1 Score: 265.0 bits (676), Expect = 7.5e-71
Identity = 144/300 (48.00%), Postives = 205/300 (68.33%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDL + SF  Y DLK+ A  D D+E+G E  + D       F E+ E VK  M  +  +
Sbjct: 1   MNDLFSSSFKKYTDLKQQAQMD-DIESGKETMNLDK------FFEDVENVKDNMKGVETL 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
              LQ +NEE K+ H  + +K LR  ++ D+  VLK+ + I+ +LE +++ NA  + +SG
Sbjct: 61  YKSLQDSNEECKTVHNAKKVKELRAKMDGDVAQVLKRVKMIKQKLEALEKANANSRNVSG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
              GS+  RTR +V +GL KKLK+LM  FQ LR RM  EYKETV RRYFT+TGE+A+E+ 
Sbjct: 121 CGPGSSTDRTRTSVVSGLGKKLKDLMDSFQGLRARMNAEYKETVERRYFTITGEQADEQT 180

Query: 181 IEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           IE +I +G  E FL +AI+E GRG++ +T+ EIQ+RH A KEIE++L+ELHQVFLDMA +
Sbjct: 181 IENLISSGESENFLQKAIQEQGRGQILDTISEIQERHDAVKEIEKNLIELHQVFLDMAAL 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VE+QG++++DIE HV  AS +V  GT  L++A++ Q+SSRKW C  ILL I++  +++IP
Sbjct: 241 VESQGQQLNDIESHVSKASSFVRRGTDQLQDAREYQKSSRKWTCYAILLFIVVFALLLIP 293

BLAST of MS017088 vs. TAIR 10
Match: AT4G03330.1 (syntaxin of plants 123 )

HSP 1 Score: 259.2 bits (661), Expect = 4.1e-69
Identity = 147/301 (48.84%), Postives = 211/301 (70.10%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDL++ SF  Y DL      D D+E+  +  S DS G++  F    E VK +M ++ EI
Sbjct: 1   MNDLISSSFKRYTDLNHQVQLD-DIES--QNVSLDS-GNLDEFFGYVESVKEDMKAVDEI 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
             +LQ ANEE+K+ H  + +K LR  ++  +  VLK+ + I+++L  ++++NAA+++++G
Sbjct: 61  HKRLQDANEESKTVHDSKAVKKLRARMDSSVTEVLKRVKMIKTKLVALEKSNAAQRKVAG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
              GS+  RTR +V +GL KKLK++M +FQ LR +M TEYKETV RRYFTVTG++A+EE 
Sbjct: 121 CGPGSSADRTRTSVVSGLGKKLKDMMDDFQRLRTKMATEYKETVERRYFTVTGQKADEET 180

Query: 181 IEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           +EK+I +G  E FL +AI+E GRG+V +T+ EIQ+RH   KEIERSLLELHQVFLDMA +
Sbjct: 181 VEKLISSGESERFLQKAIQEQGRGQVMDTLSEIQERHDTVKEIERSLLELHQVFLDMAAL 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQG  ++DIE +V  AS +V  GT  L  AK LQR++RKW C+  +L I++V V++ P
Sbjct: 241 VEAQGNMLNDIESNVSKASSFVMRGTDQLHGAKVLQRNNRKWACIATILAIVVVIVILFP 297

BLAST of MS017088 vs. TAIR 10
Match: AT1G11250.1 (syntaxin of plants 125 )

HSP 1 Score: 251.1 bits (640), Expect = 1.1e-66
Identity = 141/301 (46.84%), Postives = 203/301 (67.44%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEAGLEMASTDSGGDMVLFLEEAEKVKSEMGSIREI 60
           MNDL + SF      KK   +  D+EAG E  + D       F E+ E VK +M  +  +
Sbjct: 1   MNDLFSNSF------KKNQAQLGDVEAGQETMNLDK------FFEDVENVKDDMKGVEAL 60

Query: 61  LGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKKRISG 120
             KLQ +NEE K+ H  + +K LR  ++ D+  VLK+ + I+ +LE +++ NA  + + G
Sbjct: 61  YKKLQDSNEECKTVHNAKKVKELRAKMDGDVAMVLKRVKIIKQKLEALEKANANSRNVPG 120

Query: 121 SKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEAEEEV 180
              GS+  RTR +V +GL KKLK+LM  FQ LR RM  EYKETV RRYFT+TGE+A+E+ 
Sbjct: 121 CGPGSSTDRTRSSVVSGLGKKLKDLMDSFQGLRARMNNEYKETVERRYFTITGEKADEQT 180

Query: 181 IEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLDMAVM 240
           I+ +I +G  E FL +AI+E GRG++ +T+ EIQ+RH A KEIE++LLELHQVFLDMA +
Sbjct: 181 IDNLIASGESENFLQKAIQEQGRGQILDTISEIQERHDAVKEIEKNLLELHQVFLDMAAL 240

Query: 241 VEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFVVVIP 300
           VEAQG+++++IE HV  AS +V  GT  L++A++ Q+SSRKW C  I+L I+I  +++IP
Sbjct: 241 VEAQGQQLNNIESHVAKASSFVRRGTDQLQDAREYQKSSRKWTCYAIILFIVIFILLLIP 289

BLAST of MS017088 vs. TAIR 10
Match: AT3G11820.1 (syntaxin of plants 121 )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 133/312 (42.63%), Postives = 205/312 (65.71%), Query Frame = 0

Query: 1   MNDLMTKSFTSYVDLKKAAMKDIDLEA-GLEMAS---TDSGGDMVLFLEEAEKVKSEMGS 60
           MNDL + SF+ +   + +  +D+     G++MA+   +  G ++  F E+ E VK E+  
Sbjct: 1   MNDLFSSSFSRFRSGEPSPRRDVAGGGDGVQMANPAGSTGGVNLDKFFEDVESVKEELKE 60

Query: 61  IREILGKLQQANEETKSAHKPETLKSLRHTINVDIVTVLKKARSIRSQLEEMDRTNAAKK 120
           +  +   L   +E++K+ H  + +K LR  ++ D+   LKKA+ I+ +LE +DR NAA +
Sbjct: 61  LDRLNETLSSCHEQSKTLHNAKAVKDLRSKMDGDVGVALKKAKMIKVKLEALDRANAANR 120

Query: 121 RISGSKEGSAIYRTRMAVTNGLRKKLKELMMEFQSLRQRMMTEYKETVGRRYFTVTGEEA 180
            + G   GS+  RTR +V NGLRKKL + M  F  LR+ + +EY+ETV RRYFTVTGE  
Sbjct: 121 SLPGCGPGSSSDRTRTSVLNGLRKKLMDSMDSFNRLRELISSEYRETVQRRYFTVTGENP 180

Query: 181 EEEVIEKIIENG-GEEFLGRAIEEHGRGKVAETVVEIQDRHGAAKEIERSLLELHQVFLD 240
           +E  ++++I  G  E FL +AI+E GRG+V +T+ EIQ+RH A K+IE++L ELHQVFLD
Sbjct: 181 DERTLDRLISTGESERFLQKAIQEQGRGRVLDTINEIQERHDAVKDIEKNLRELHQVFLD 240

Query: 241 MAVMVEAQGEKMDDIEHHVINASQYVTDGTKDLKNAKDLQRSSRKWMCLGILLLILIVFV 300
           MAV+VE QG ++DDIE HV  AS ++  GT  L+ A+  Q+++RKW C+ I++LI+I+ V
Sbjct: 241 MAVLVEHQGAQLDDIESHVGRASSFIRGGTDQLQTARVYQKNTRKWTCIAIIILIIIITV 300

Query: 301 VVIPIAVSFGSS 308
           VV+ +   + +S
Sbjct: 301 VVLAVLKPWNNS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156189.13.4e-15899.67syntaxin-related protein KNOLLE [Momordica charantia][more]
XP_038881435.11.1e-14089.00syntaxin-related protein KNOLLE [Benincasa hispida][more]
KAG7027497.12.4e-14089.29Syntaxin-related protein KNOLLE, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_004147530.11.2e-13989.32syntaxin-related protein KNOLLE [Cucumis sativus] >KGN53948.1 hypothetical prote... [more]
XP_022925127.12.7e-13988.64syntaxin-related protein KNOLLE-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q423745.5e-10367.09Syntaxin-related protein KNOLLE OS=Arabidopsis thaliana OX=3702 GN=KN PE=1 SV=1[more]
O647911.1e-6948.00Syntaxin-124 OS=Arabidopsis thaliana OX=3702 GN=SYP124 PE=2 SV=1[more]
Q9ZQZ85.8e-6848.84Syntaxin-123 OS=Arabidopsis thaliana OX=3702 GN=SYP123 PE=2 SV=1[more]
Q9SXB01.6e-6546.84Syntaxin-125 OS=Arabidopsis thaliana OX=3702 GN=SYP125 PE=2 SV=1[more]
Q9ZSD41.5e-6042.63Syntaxin-121 OS=Arabidopsis thaliana OX=3702 GN=SYP121 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DSL91.6e-15899.67syntaxin-related protein KNOLLE OS=Momordica charantia OX=3673 GN=LOC111023138 P... [more]
A0A0A0KWP35.9e-14089.32t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365... [more]
A0A5A7UY081.3e-13989.32Syntaxin-related protein KNOLLE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1EAY41.3e-13988.64syntaxin-related protein KNOLLE-like OS=Cucurbita moschata OX=3662 GN=LOC1114324... [more]
A0A1S3B4451.3e-13989.32syntaxin-related protein KNOLLE OS=Cucumis melo OX=3656 GN=LOC103485972 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G08560.13.9e-10467.09syntaxin of plants 111 [more]
AT1G61290.17.5e-7148.00syntaxin of plants 124 [more]
AT4G03330.14.1e-6948.84syntaxin of plants 123 [more]
AT1G11250.11.1e-6646.84syntaxin of plants 125 [more]
AT3G11820.11.1e-6142.63syntaxin of plants 121 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 135..155
NoneNo IPR availableGENE3D1.20.58.70coord: 40..167
e-value: 4.1E-21
score: 77.2
NoneNo IPR availableGENE3D1.20.5.110coord: 204..305
e-value: 4.5E-30
score: 105.5
NoneNo IPR availablePANTHERPTHR19957SYNTAXINcoord: 4..300
NoneNo IPR availablePANTHERPTHR19957:SF251SYNTAXIN-RELATED PROTEIN KNOLLEcoord: 4..300
NoneNo IPR availableCDDcd15848SNARE_syntaxin1-likecoord: 208..269
e-value: 1.77114E-22
score: 86.4357
IPR000727Target SNARE coiled-coil homology domainSMARTSM00397tSNARE_6coord: 204..271
e-value: 1.2E-17
score: 74.7
IPR000727Target SNARE coiled-coil homology domainPFAMPF05739SNAREcoord: 247..297
e-value: 1.4E-14
score: 53.8
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 209..271
score: 16.644669
IPR006011Syntaxin, N-terminal domainSMARTSM00503SynN_4coord: 35..161
e-value: 1.1E-33
score: 127.8
IPR006011Syntaxin, N-terminal domainPFAMPF00804Syntaxincoord: 42..244
e-value: 5.7E-65
score: 218.8
IPR006011Syntaxin, N-terminal domainCDDcd00179SynNcoord: 40..173
e-value: 1.10775E-30
score: 111.222
IPR006012Syntaxin/epimorphin, conserved sitePROSITEPS00914SYNTAXINcoord: 215..255
IPR010989SNARESUPERFAMILY47661t-snare proteinscoord: 38..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS017088.1MS017088.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0016020 membrane
molecular_function GO:0005484 SNAP receptor activity