MS017086 (gene) Bitter gourd (TR) v1

Overview


Name	MS017086
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	B3 domain-containing protein At1g05920-like
Location	scaffold197: 1325753 .. 1326589 (+)
RNA-Seq Expression	MS017086
Synteny	MS017086

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAAGAAATTCCACTAACAACCGGCGAAGTCGACGAAAATCAAGAAAAGAAAGAGGTTGCAGAAGAAGAAACCATCACAATAATCGATCTCACTAACGACGACAATGAACGAATGCCACTAAAAAAGAGGAAGAAGAGGAAGAGGAGGGATATGGAAGCAGACCTGAAGCAACTGAGATTATTGAGAGAAAAGCTTCTGAAACGCCCAATCGTCGAATCGAAAGAAGTTCAAAGTCTCGAAGCGGCACCACCGGCCGCGGAGTGCGACAGCGAGAGCGAGGGCGAGGGCGAGGTCATCACCGATCGAGAATGCGCCCTAATCATCGAGGCGATGCCGCAATTAGCGGAAGTCCGGGGAGCGGTGAGCTACGACCTGCCGCAGGAGATCCAGAGAGCGATGAATTTCATGGGAGGATGTTGTTCGGGCCCTAATTTCGTGATGGCGAAGAAATTGGGGAAAGCGGATGTGGACGAGAGATGCAATCGGCTGGTGATTCCGCAGAGGAAGATTAGAGAGAACTTTATGGAACTGGAGGAGGAGGAAAAATTGAACCAGGAGAAGTGGATGATGGTGGAGATCATCGAGCCGGACTCTTCTGTGAGTTTGATGACGCTGAGCAAGTGGGAGACGAGGAAGGGGCCGGCGTATGTGATGATCACGGAGTGGCACGGCCTGGTGGAGAGGAACGAGTTGAAGGAGGGAGATACGGTGAATCTATGGAGTTTCCGCGCCGGCGGCGGTGGCGGCGGTGGCGGCGGCGGCCATGGGAGGCTCTGCTTCTTGCTCGTTGAAGAAGAAGGAGAAGAAGGGGGAAAATTAGGACCTACGATTGAG

mRNA sequence

AAGAAAGAAATTCCACTAACAACCGGCGAAGTCGACGAAAATCAAGAAAAGAAAGAGGTTGCAGAAGAAGAAACCATCACAATAATCGATCTCACTAACGACGACAATGAACGAATGCCACTAAAAAAGAGGAAGAAGAGGAAGAGGAGGGATATGGAAGCAGACCTGAAGCAACTGAGATTATTGAGAGAAAAGCTTCTGAAACGCCCAATCGTCGAATCGAAAGAAGTTCAAAGTCTCGAAGCGGCACCACCGGCCGCGGAGTGCGACAGCGAGAGCGAGGGCGAGGGCGAGGTCATCACCGATCGAGAATGCGCCCTAATCATCGAGGCGATGCCGCAATTAGCGGAAGTCCGGGGAGCGGTGAGCTACGACCTGCCGCAGGAGATCCAGAGAGCGATGAATTTCATGGGAGGATGTTGTTCGGGCCCTAATTTCGTGATGGCGAAGAAATTGGGGAAAGCGGATGTGGACGAGAGATGCAATCGGCTGGTGATTCCGCAGAGGAAGATTAGAGAGAACTTTATGGAACTGGAGGAGGAGGAAAAATTGAACCAGGAGAAGTGGATGATGGTGGAGATCATCGAGCCGGACTCTTCTGTGAGTTTGATGACGCTGAGCAAGTGGGAGACGAGGAAGGGGCCGGCGTATGTGATGATCACGGAGTGGCACGGCCTGGTGGAGAGGAACGAGTTGAAGGAGGGAGATACGGTGAATCTATGGAGTTTCCGCGCCGGCGGCGGTGGCGGCGGTGGCGGCGGCGGCCATGGGAGGCTCTGCTTCTTGCTCGTTGAAGAAGAAGGAGAAGAAGGGGGAAAATTAGGACCTACGATTGAG

Coding sequence (CDS)

AAGAAAGAAATTCCACTAACAACCGGCGAAGTCGACGAAAATCAAGAAAAGAAAGAGGTTGCAGAAGAAGAAACCATCACAATAATCGATCTCACTAACGACGACAATGAACGAATGCCACTAAAAAAGAGGAAGAAGAGGAAGAGGAGGGATATGGAAGCAGACCTGAAGCAACTGAGATTATTGAGAGAAAAGCTTCTGAAACGCCCAATCGTCGAATCGAAAGAAGTTCAAAGTCTCGAAGCGGCACCACCGGCCGCGGAGTGCGACAGCGAGAGCGAGGGCGAGGGCGAGGTCATCACCGATCGAGAATGCGCCCTAATCATCGAGGCGATGCCGCAATTAGCGGAAGTCCGGGGAGCGGTGAGCTACGACCTGCCGCAGGAGATCCAGAGAGCGATGAATTTCATGGGAGGATGTTGTTCGGGCCCTAATTTCGTGATGGCGAAGAAATTGGGGAAAGCGGATGTGGACGAGAGATGCAATCGGCTGGTGATTCCGCAGAGGAAGATTAGAGAGAACTTTATGGAACTGGAGGAGGAGGAAAAATTGAACCAGGAGAAGTGGATGATGGTGGAGATCATCGAGCCGGACTCTTCTGTGAGTTTGATGACGCTGAGCAAGTGGGAGACGAGGAAGGGGCCGGCGTATGTGATGATCACGGAGTGGCACGGCCTGGTGGAGAGGAACGAGTTGAAGGAGGGAGATACGGTGAATCTATGGAGTTTCCGCGCCGGCGGCGGTGGCGGCGGTGGCGGCGGCGGCCATGGGAGGCTCTGCTTCTTGCTCGTTGAAGAAGAAGGAGAAGAAGGGGGAAAATTAGGACCTACGATTGAG

Protein sequence

KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLREKLLKRPIVESKEVQSLEAAPPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRGAVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEEEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLWSFRAGGGGGGGGGGHGRLCFLLVEEEGEEGGKLGPTIE

Homology

[Close]

BLAST of MS017086 vs. NCBI nr
Match: XP_022156209.1 (B3 domain-containing protein At1g05920-like [Momordica charantia])

HSP 1 Score: 531.2 bits (1367), Expect = 5.5e-147
Identity = 277/279 (99.28%), Postives = 278/279 (99.64%), Query Frame = 0


Query: 1   KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLR 60
           KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLR
Sbjct: 110 KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLR 169

Query: 61  LLREKLLKRPIVESKEVQSLEAAPPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRG 120
           LLREKLLKRPIVESKEVQSLEAAPPAAEC+SESEGEGEVITDRECALIIEAMPQLAEVRG
Sbjct: 170 LLREKLLKRPIVESKEVQSLEAAPPAAECESESEGEGEVITDRECALIIEAMPQLAEVRG 229

Query: 121 AVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEE 180
           AVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEE
Sbjct: 230 AVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEE 289

Query: 181 EEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNL 240
           EEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNL
Sbjct: 290 EEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNL 349

Query: 241 WSFRAGGGGGGGGGGHGRLCFLLVEEEGEEGGKLGPTIE 280
           WSFRAGGGGGGGGGGHGRLCFLLV EEGEEGGKLGPTIE
Sbjct: 350 WSFRAGGGGGGGGGGHGRLCFLLV-EEGEEGGKLGPTIE 387

[Close]

BLAST of MS017086 vs. NCBI nr
Match: XP_023544949.1 (B3 domain-containing protein At2g31420-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-51
Identity = 134/271 (49.45%), Postives = 175/271 (64.58%), Query Frame = 0


Query: 5   PLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLRE 64
           P  T +  ++Q+++ V ++E +++I      N+R P                K+L+LL+E
Sbjct: 17  PNLTSDGVQDQDRESVEDDEHLSLIKRKRSPNKRTP----------------KRLKLLKE 76

Query: 65  KLLKRPIVESKEVQSLEAA--PPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRGAV 124
           + LKRPIV+S     LEA+   P    D+E+E E +V  D +  L+I     + E    +
Sbjct: 77  RSLKRPIVQSIASPVLEASAEQPDIIFDAETESESDVNDDLD-DLLIGCKQDVHEFDRIL 136

Query: 125 SYDLPQEIQRAMNFMGGCC-SGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEE 184
              LPQ+ +  M FMG    S PNF++ K+L K DVDER NRLVIPQRK+R NF++LEEE
Sbjct: 137 VDGLPQDYKSLMIFMGASSNSSPNFLLQKRLKKGDVDERRNRLVIPQRKMRNNFLDLEEE 196

Query: 185 EKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLW 244
           EKLN E+W MVE+IEPD SVSL+TLSKWET  G AYV ITEW+GLVERNELKEGD V LW
Sbjct: 197 EKLNMERWWMVEVIEPDCSVSLITLSKWETPSGAAYVFITEWNGLVERNELKEGDLVQLW 256

Query: 245 SFRAGGGGGGGGGGHGRLCFLLVE--EEGEE 271
           SFR    G GG G HGRLCF+L+E  EEG++
Sbjct: 257 SFRV-HVGEGGDGDHGRLCFMLLEICEEGDD 269

[Close]

BLAST of MS017086 vs. NCBI nr
Match: XP_038883177.1 (B3 domain-containing protein At1g05920-like [Benincasa hispida])

HSP 1 Score: 213.4 bits (542), Expect = 2.5e-51
Identity = 139/280 (49.64%), Postives = 186/280 (66.43%), Query Frame = 0


Query: 8   TGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLREKLL 67
           TG+VD++QE    A+E T T  +L +D+++ +PL KRK+ K   +    K+ +LLRE+ L
Sbjct: 9   TGDVDKDQETTVSAKEGT-TDRNL-SDEDDHLPLIKRKRTK---VHISSKRHKLLRERRL 68

Query: 68  KRPI-------VESKEVQSLEAAPPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRG 127
           KRP+       +   + Q  E+        +ESE + +   +RE AL I  +  +AE   
Sbjct: 69  KRPLPLRIADPIPKFDEQPEESPRKNLIFVAESESDSDTDRNRE-ALAIAGLADIAETNQ 128

Query: 128 AVSYDLPQEIQRAMNFMGGCCS-GPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELE 187
            +  ++P+E +  M FMGG  S  PN V+ K+L K DV+ER NRL+IPQRKI+ NF+E+E
Sbjct: 129 ELIQNIPEEYKNLMIFMGGSRSASPNLVLQKRLRKGDVEERRNRLLIPQRKIKTNFLEVE 188

Query: 188 EEEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVN 247
           EEEKLN++ W MVEIIEPD SVSL+TLSKWET KG AYV ITEW+GLVERNELKEGD + 
Sbjct: 189 EEEKLNRDIWWMVEIIEPDCSVSLITLSKWETWKGVAYVFITEWNGLVERNELKEGDLMQ 248

Query: 248 LWSFRAGGGGGGGGGGHGRLCFLLVE--EEGEEGGKLGPT 278
           LWSFRA    GGGGG  GRLCF+L+E  +  EE  K+ P+
Sbjct: 249 LWSFRA---AGGGGGRRGRLCFMLLELDDNREENDKVTPS 279

[Close]

BLAST of MS017086 vs. NCBI nr
Match: KAG7033674.1 (B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 211.5 bits (537), Expect = 9.6e-51
Identity = 132/271 (48.71%), Postives = 174/271 (64.21%), Query Frame = 0


Query: 5   PLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLRE 64
           P  T +  ++Q+++ V ++E +++I      N+R P                K+L+LL+E
Sbjct: 17  PNLTRDGVQDQDQESVEDDEHLSLIKRKRSPNKRTP----------------KRLKLLKE 76

Query: 65  KLLKRPIVESKEVQSLEAA--PPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRGAV 124
           + LKRPIV+S     LEA+   P    D+E+E + +V  D +  L+I     + E    +
Sbjct: 77  RSLKRPIVQSIGSPVLEASAEQPDIIFDAETESDSDVNDDLD-DLLIGCKQDVHEFDRIL 136

Query: 125 SYDLPQEIQRAMNFMGGCC-SGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEE 184
           +  LPQ  +  M FMG    S PNF++ K+L K DVDER NRLVIPQRK+R NF++LEEE
Sbjct: 137 ADGLPQNYKSLMIFMGASSKSSPNFLLQKRLKKGDVDERRNRLVIPQRKMRNNFLDLEEE 196

Query: 185 EKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLW 244
           EKLN E+W +VE+IEPD SVSLMTLSKWET  G AYV ITEW+GLVERNELKEGD V LW
Sbjct: 197 EKLNMERWWIVEVIEPDCSVSLMTLSKWETPSGAAYVFITEWNGLVERNELKEGDLVQLW 256

Query: 245 SFRAGGGGGGGGGGHGRLCFLLVE--EEGEE 271
           SFR    G GG   HGRLCF+L+E  EEG++
Sbjct: 257 SFRV--HGSGGDDDHGRLCFMLLEICEEGDD 268

[Close]

BLAST of MS017086 vs. NCBI nr
Match: XP_022950597.1 (B3 domain-containing protein At2g31420-like [Cucurbita moschata])

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-50
Identity = 132/271 (48.71%), Postives = 174/271 (64.21%), Query Frame = 0


Query: 5   PLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLRE 64
           P  T +  ++Q+++ V ++E +++I      N+R P                K+L+LL+E
Sbjct: 17  PNLTRDGVQDQDQESVEDDEHLSLIKRKRSPNKRTP----------------KRLKLLKE 76

Query: 65  KLLKRPIVESKEVQSLEAA--PPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRGAV 124
           + LKRPIV+S     LEA+   P    D+E+E + +V  D +  L+I     + E    +
Sbjct: 77  RSLKRPIVQSIASPVLEASAEQPDIIFDAETESDSDVNDDLD-DLLIGFKQDVHEFDRIL 136

Query: 125 SYDLPQEIQRAMNFMGGCC-SGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEE 184
           +  LPQ  +  M FMG    S PNF++ K+L K DVDER NRLVIPQRK+R NF++LEEE
Sbjct: 137 ADGLPQNYKSLMIFMGASSKSSPNFLLQKRLKKGDVDERRNRLVIPQRKMRNNFLDLEEE 196

Query: 185 EKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLW 244
           EKLN E+W +VE+IEPD SVSLMTLSKWET  G AYV ITEW+GLVERNELKEGD V LW
Sbjct: 197 EKLNMERWWIVEVIEPDCSVSLMTLSKWETPSGAAYVFITEWNGLVERNELKEGDLVQLW 256

Query: 245 SFRAGGGGGGGGGGHGRLCFLLVE--EEGEE 271
           SFR     GGG   HGRLCF+L+E  EEG++
Sbjct: 257 SFRV---HGGGDDDHGRLCFMLLEICEEGDD 267

[Close]

BLAST of MS017086 vs. ExPASy Swiss-Prot
Match: Q5RM09 (B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05920 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.4e-07
Identity = 72/251 (28.69%), Postives = 109/251 (43.43%), Query Frame = 0


Query: 42  KKRKKRKRRDMEADLKQLRLLREKLLKRPI-------VESKEVQSLEAAPPAAECDSESE 101
           K+R++   RD     K +  +R+   KR          E       + AP  A       
Sbjct: 71  KRREEMISRDNRTKPKTVAQVRDAKRKRTFDHVPRGTREPHAYLRNDPAPQVASVPKSVP 130

Query: 102 GEGEVITDRECALIIEAMPQLAEVRGAVSYDL--------PQEIQRAMNFMGGCCSGPNF 161
            E +VI DR    I+  +P+    +   SY+         PQ + + M+ M G    P  
Sbjct: 131 EEKDVILDR----ILSNVPRR---KKTTSYEFVPPKHPQEPQWLLQVMSRMNG-AGDPKL 190

Query: 162 VMAKKLGKADVDERCNRLVIPQRKIREN-FMELEE-----EEKLNQEKWMMVE--IIEPD 221
           ++ K L   DVD R NRL IP   + +N F+ L+E     E+++  E  M V   +++  
Sbjct: 191 IIEKNLDSNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDEDEITNEGNMGVAAFLVDQR 250

Query: 222 SSVSLMTLSKW--ETRKGPAY---VMITEWHGLVERNELKEGDTVNLWSFRAGGGGGGGG 265
           +    M   +W   T  G +Y   V+  EW  +VE N LKEGD ++LWSFR+        
Sbjct: 251 TKKWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRS-------- 303

[Close]

BLAST of MS017086 vs. ExPASy Swiss-Prot
Match: Q9LRX6 (Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 GN=At3g24850 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 8.3e-05
Identity = 38/135 (28.15%), Postives = 61/135 (45.19%), Query Frame = 0


Query: 125 DLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEEEKL 184
           D P+ + + M  M         +  K L   D++   NRL +P   + +N      E ++
Sbjct: 208 DTPKWLVKVMRDMKE-AKDAKLIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRI 267

Query: 185 ------NQEKWMMVEIIEPDSSVSL-MTLSKWETRKGPA-----YVMITEWHGLVERNEL 244
                 N +K  +  I+    SV   + L +WE +K        Y +I  W+ +VE N L
Sbjct: 268 IKEDINNNKKIGVGAILVDQRSVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGL 327

Query: 245 KEGDTVNLWSFRAGG 248
           KEGD +++WSFR  G
Sbjct: 328 KEGDNISVWSFRCRG 341

[Close]

BLAST of MS017086 vs. ExPASy Swiss-Prot
Match: Q9FLV7 (B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24050 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.1e-04
Identity = 37/113 (32.74%), Postives = 53/113 (46.90%), Query Frame = 0


Query: 147 VMAKKLGKADVDERCNRLVIP-QRKIRENFMELEE----EEKLNQEKWMMVEIIEPDSSV 206
           +  K L   DV+   NRL +P    +R +F+   E     E +N    + V  I  D   
Sbjct: 219 IFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIIINEDINNNNKIGVGAILVDQRC 278

Query: 207 SL--MTLSKWETRKGP-----AYVMITEWHGLVERNELKEGDTVNLWSFRAGG 248
               + L +WE +K       +Y +I  W+ +VE N LKEGD + LWSFR  G
Sbjct: 279 KKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLKEGDNITLWSFRCCG 331

[Close]

BLAST of MS017086 vs. ExPASy Swiss-Prot
Match: Q9LSF7 (B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25182 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.8e-04
Identity = 36/113 (31.86%), Postives = 56/113 (49.56%), Query Frame = 0


Query: 147 VMAKKLGKADVDERCNRLVIP-QRKIRENFMELEE----EEKLNQEKWMMVEIIEPDSSV 206
           +  K L   DV+   NRL +P    +R +F+   E    ++ +  +K + V  I  D   
Sbjct: 216 IFEKTLFVTDVNPTQNRLSMPFNNLLRNDFLTSVESRIIDKDIKNDKKIGVGAILVDQRC 275

Query: 207 SL--MTLSKWETRKGPA-----YVMITEWHGLVERNELKEGDTVNLWSFRAGG 248
               + L +WE +K        Y +I  W+ +VE N LKEGD ++LWSFR+ G
Sbjct: 276 KKWGVMLKRWEMKKESGKGSWNYNLICGWNDIVEANGLKEGDNISLWSFRSCG 328

[Close]

BLAST of MS017086 vs. ExPASy TrEMBL
Match: A0A6J1DSR8 (B3 domain-containing protein At1g05920-like OS=Momordica charantia OX=3673 GN=LOC111023152 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.6e-147
Identity = 277/279 (99.28%), Postives = 278/279 (99.64%), Query Frame = 0


Query: 1   KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLR 60
           KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLR
Sbjct: 110 KKEIPLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLR 169

Query: 61  LLREKLLKRPIVESKEVQSLEAAPPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRG 120
           LLREKLLKRPIVESKEVQSLEAAPPAAEC+SESEGEGEVITDRECALIIEAMPQLAEVRG
Sbjct: 170 LLREKLLKRPIVESKEVQSLEAAPPAAECESESEGEGEVITDRECALIIEAMPQLAEVRG 229

Query: 121 AVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEE 180
           AVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEE
Sbjct: 230 AVSYDLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEE 289

Query: 181 EEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNL 240
           EEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNL
Sbjct: 290 EEKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNL 349

Query: 241 WSFRAGGGGGGGGGGHGRLCFLLVEEEGEEGGKLGPTIE 280
           WSFRAGGGGGGGGGGHGRLCFLLV EEGEEGGKLGPTIE
Sbjct: 350 WSFRAGGGGGGGGGGHGRLCFLLV-EEGEEGGKLGPTIE 387

[Close]

BLAST of MS017086 vs. ExPASy TrEMBL
Match: A0A6J1GF95 (B3 domain-containing protein At2g31420-like OS=Cucurbita moschata OX=3662 GN=LOC111453649 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 6.1e-51
Identity = 132/271 (48.71%), Postives = 174/271 (64.21%), Query Frame = 0


Query: 5   PLTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLRE 64
           P  T +  ++Q+++ V ++E +++I      N+R P                K+L+LL+E
Sbjct: 17  PNLTRDGVQDQDQESVEDDEHLSLIKRKRSPNKRTP----------------KRLKLLKE 76

Query: 65  KLLKRPIVESKEVQSLEAA--PPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRGAV 124
           + LKRPIV+S     LEA+   P    D+E+E + +V  D +  L+I     + E    +
Sbjct: 77  RSLKRPIVQSIASPVLEASAEQPDIIFDAETESDSDVNDDLD-DLLIGFKQDVHEFDRIL 136

Query: 125 SYDLPQEIQRAMNFMGGCC-SGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEE 184
           +  LPQ  +  M FMG    S PNF++ K+L K DVDER NRLVIPQRK+R NF++LEEE
Sbjct: 137 ADGLPQNYKSLMIFMGASSKSSPNFLLQKRLKKGDVDERRNRLVIPQRKMRNNFLDLEEE 196

Query: 185 EKLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLW 244
           EKLN E+W +VE+IEPD SVSLMTLSKWET  G AYV ITEW+GLVERNELKEGD V LW
Sbjct: 197 EKLNMERWWIVEVIEPDCSVSLMTLSKWETPSGAAYVFITEWNGLVERNELKEGDLVQLW 256

Query: 245 SFRAGGGGGGGGGGHGRLCFLLVE--EEGEE 271
           SFR     GGG   HGRLCF+L+E  EEG++
Sbjct: 257 SFRV---HGGGDDDHGRLCFMLLEICEEGDD 267

[Close]

BLAST of MS017086 vs. ExPASy TrEMBL
Match: A0A6J1ITM1 (uncharacterized protein LOC111478299 OS=Cucurbita maxima OX=3661 GN=LOC111478299 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 6.7e-50
Identity = 129/269 (47.96%), Postives = 172/269 (63.94%), Query Frame = 0


Query: 6   LTTGEVDENQEKKEVAEEETITIIDLTNDDNERMPLKKRKKRKRRDMEADLKQLRLLREK 65
           ++ G  D++Q+++ V ++E +++I      N+R P                K+L+LL+E+
Sbjct: 20  ISDGVQDQDQDQESVEDDERLSLIKRKRSPNKRTP----------------KRLKLLKER 79

Query: 66  LLKRPIVESKEVQSLEAA--PPAAECDSESEGEGEVITDRECALIIEAMPQLAEVRGAVS 125
            LKRPIV+S     LEA+   P    D+E+E + +V  D    L+I     + E    ++
Sbjct: 80  SLKRPIVQSIASPVLEASAEQPDIIFDTETESDSDVKDDLG-DLLIGFKQDVYEFDRILA 139

Query: 126 YDLPQEIQRAMNFMGGCC-SGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEEE 185
             L Q+ +  M FMG    S P+F++ K+L K DVDER NRLVIPQRK+R NF++LEEEE
Sbjct: 140 DGLHQDYKSLMIFMGASSNSSPSFLLQKRLKKGDVDERRNRLVIPQRKMRNNFLDLEEEE 199

Query: 186 KLNQEKWMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLWS 245
           KLN E+W MVE+IEPD SVSLMTLSKWET  G AYV ITEW+GLVERNELKEGD + LWS
Sbjct: 200 KLNMERWWMVEVIEPDCSVSLMTLSKWETPSGAAYVFITEWNGLVERNELKEGDLIQLWS 259

Query: 246 FRAGGGGGGGGGGHGRLCFLLVEE-EGEE 271
           FR        GG HGRLCF+L+E  EG+E
Sbjct: 260 FRV------HGGDHGRLCFMLLEICEGDE 265

[Close]

BLAST of MS017086 vs. ExPASy TrEMBL
Match: A0A5D3C998 (B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00460 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.2e-46
Identity = 121/259 (46.72%), Postives = 168/259 (64.86%), Query Frame = 0


Query: 15  QEKKEVAEEETITIIDLT-NDDNERMPLKKRKKRKRRDMEADLKQLRLLREKLLKRPIVE 74
           Q+++E        I D   +DD++ +PL KRK+ + + +    ++ +  REKLLKRP+  
Sbjct: 15  QQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQL---ARRHKRDREKLLKRPLKH 74

Query: 75  --SKEVQSLEAAPPAAEC--DSESEGEGEVITDRECALIIEAMPQLAEVRGAVSYDLPQE 134
             +  +   +  P   +   D+E+E EG+   ++E  L   A+ ++ E       ++P+E
Sbjct: 75  PVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQE-TLTFMALAEIVESEREFLENIPEE 134

Query: 135 IQRAMNFMGGCCS-GPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEEEKLNQEK 194
            ++ M FMGG     P+ VM K+L K DV+ER NRL+IPQRK R  F+E+EEEEKLN + 
Sbjct: 135 YKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEVEEEEKLNTDV 194

Query: 195 WMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLWSFRAGGG 254
           W MVEIIEPD +VSL+TLSKWETRKG AYV+ITEW+GLVERN+LKEGD + LWSFRA   
Sbjct: 195 WWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQLWSFRA--- 254

Query: 255 GGGGGGGHGRLCFLLVEEE 268
               GGG GRLCF+L+E E
Sbjct: 255 ----GGGRGRLCFMLLEVE 262

[Close]

BLAST of MS017086 vs. ExPASy TrEMBL
Match: A0A5A7SSE3 (B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold7305G00010 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 2.0e-46
Identity = 120/259 (46.33%), Postives = 168/259 (64.86%), Query Frame = 0


Query: 15  QEKKEVAEEETITIIDLT-NDDNERMPLKKRKKRKRRDMEADLKQLRLLREKLLKRPIVE 74
           Q+++E        I D   +DD++ +PL KRK+ + + +    ++ +  REK+LKRP+  
Sbjct: 15  QQEQETTVSTNGEITDQNPSDDDDHLPLIKRKRTRMKQL---ARRHKRDREKILKRPLKH 74

Query: 75  --SKEVQSLEAAPPAAEC--DSESEGEGEVITDRECALIIEAMPQLAEVRGAVSYDLPQE 134
             +  +   +  P   +   D+E+E EG+   ++E  L   A+ ++ E       ++P+E
Sbjct: 75  PVNDPIPKSDEEPQGKDLVFDTETESEGDADQNQE-TLTFMALAEIVESEREFLENIPEE 134

Query: 135 IQRAMNFMGGCCS-GPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEEEKLNQEK 194
            ++ M FMGG     P+ VM K+L K DV+ER NRL+IPQRK R  F+E+EEEEKLN + 
Sbjct: 135 YKKLMIFMGGSWDVCPSLVMEKRLKKGDVEERRNRLLIPQRKTRTTFLEVEEEEKLNTDV 194

Query: 195 WMMVEIIEPDSSVSLMTLSKWETRKGPAYVMITEWHGLVERNELKEGDTVNLWSFRAGGG 254
           W MVEIIEPD +VSL+TLSKWETRKG AYV+ITEW+GLVERN+LKEGD + LWSFRA   
Sbjct: 195 WWMVEIIEPDCTVSLITLSKWETRKGVAYVLITEWNGLVERNDLKEGDLMQLWSFRA--- 254

Query: 255 GGGGGGGHGRLCFLLVEEE 268
               GGG GRLCF+L+E E
Sbjct: 255 ----GGGRGRLCFMLLEVE 262

[Close]

BLAST of MS017086 vs. TAIR 10
Match: AT1G05920.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 58.5 bits (140), Expect = 9.7e-09
Identity = 72/251 (28.69%), Postives = 109/251 (43.43%), Query Frame = 0


Query: 42  KKRKKRKRRDMEADLKQLRLLREKLLKRPI-------VESKEVQSLEAAPPAAECDSESE 101
           K+R++   RD     K +  +R+   KR          E       + AP  A       
Sbjct: 71  KRREEMISRDNRTKPKTVAQVRDAKRKRTFDHVPRGTREPHAYLRNDPAPQVASVPKSVP 130

Query: 102 GEGEVITDRECALIIEAMPQLAEVRGAVSYDL--------PQEIQRAMNFMGGCCSGPNF 161
            E +VI DR    I+  +P+    +   SY+         PQ + + M+ M G    P  
Sbjct: 131 EEKDVILDR----ILSNVPRR---KKTTSYEFVPPKHPQEPQWLLQVMSRMNG-AGDPKL 190

Query: 162 VMAKKLGKADVDERCNRLVIPQRKIREN-FMELEE-----EEKLNQEKWMMVE--IIEPD 221
           ++ K L   DVD R NRL IP   + +N F+ L+E     E+++  E  M V   +++  
Sbjct: 191 IIEKNLDSNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDEDEITNEGNMGVAAFLVDQR 250

Query: 222 SSVSLMTLSKW--ETRKGPAY---VMITEWHGLVERNELKEGDTVNLWSFRAGGGGGGGG 265
           +    M   +W   T  G +Y   V+  EW  +VE N LKEGD ++LWSFR+        
Sbjct: 251 TKKWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRS-------- 303

[Close]

BLAST of MS017086 vs. TAIR 10
Match: AT3G24850.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 49.3 bits (116), Expect = 5.9e-06
Identity = 38/135 (28.15%), Postives = 61/135 (45.19%), Query Frame = 0


Query: 125 DLPQEIQRAMNFMGGCCSGPNFVMAKKLGKADVDERCNRLVIPQRKIRENFMELEEEEKL 184
           D P+ + + M  M         +  K L   D++   NRL +P   + +N      E ++
Sbjct: 208 DTPKWLVKVMRDMKE-AKDAKLIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRI 267

Query: 185 ------NQEKWMMVEIIEPDSSVSL-MTLSKWETRKGPA-----YVMITEWHGLVERNEL 244
                 N +K  +  I+    SV   + L +WE +K        Y +I  W+ +VE N L
Sbjct: 268 IKEDINNNKKIGVGAILVDQRSVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGL 327

Query: 245 KEGDTVNLWSFRAGG 248
           KEGD +++WSFR  G
Sbjct: 328 KEGDNISVWSFRCRG 341

[Close]

BLAST of MS017086 vs. TAIR 10
Match: AT5G24050.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 48.9 bits (115), Expect = 7.7e-06
Identity = 37/113 (32.74%), Postives = 53/113 (46.90%), Query Frame = 0


Query: 147 VMAKKLGKADVDERCNRLVIP-QRKIRENFMELEE----EEKLNQEKWMMVEIIEPDSSV 206
           +  K L   DV+   NRL +P    +R +F+   E     E +N    + V  I  D   
Sbjct: 219 IFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIIINEDINNNNKIGVGAILVDQRC 278

Query: 207 SL--MTLSKWETRKGP-----AYVMITEWHGLVERNELKEGDTVNLWSFRAGG 248
               + L +WE +K       +Y +I  W+ +VE N LKEGD + LWSFR  G
Sbjct: 279 KKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLKEGDNITLWSFRCCG 331

The following BLAST results are available for this feature:

BLAST of MS017086 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156209.1	5.5e-147	99.28	B3 domain-containing protein At1g05920-like [Momordica charantia]	[more]
XP_023544949.1	1.5e-51	49.45	B3 domain-containing protein At2g31420-like [Cucurbita pepo subsp. pepo]	[more]
XP_038883177.1	2.5e-51	49.64	B3 domain-containing protein At1g05920-like [Benincasa hispida]	[more]
KAG7033674.1	9.6e-51	48.71	B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
XP_022950597.1	1.3e-50	48.71	B3 domain-containing protein At2g31420-like [Cucurbita moschata]	[more]

BLAST of MS017086 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5RM09	1.4e-07	28.69	B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05...	[more]
Q9LRX6	8.3e-05	28.15	Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FLV7	1.1e-04	32.74	B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24...	[more]
Q9LSF7	1.8e-04	31.86	B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25...	[more]

BLAST of MS017086 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DSR8	2.6e-147	99.28	B3 domain-containing protein At1g05920-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1GF95	6.1e-51	48.71	B3 domain-containing protein At2g31420-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1ITM1	6.7e-50	47.96	uncharacterized protein LOC111478299 OS=Cucurbita maxima OX=3661 GN=LOC111478299...	[more]
A0A5D3C998	1.2e-46	46.72	B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A5A7SSE3	2.0e-46	46.33	B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]

BLAST of MS017086 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05920.1	9.7e-09	28.69	Domain of unknown function (DUF313)	[more]
AT3G24850.1	5.9e-06	28.15	Domain of unknown function (DUF313)	[more]
AT5G24050.1	7.7e-06	32.74	Domain of unknown function (DUF313)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 42..69
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..52
None	No IPR available	PANTHER	PTHR31541:SF25	DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATED	coord: 12..270
IPR005508	B3 domain-containing protein At2g31720-like	PFAM	PF03754	DUF313	coord: 127..228 e-value: 5.5E-9 score: 36.2
IPR005508	B3 domain-containing protein At2g31720-like	PANTHER	PTHR31541	B3 DOMAIN PLANT PROTEIN-RELATED	coord: 12..270
IPR015300	DNA-binding pseudobarrel domain superfamily	GENE3D	2.40.330.10		coord: 142..248 e-value: 7.7E-17 score: 63.1
IPR015300	DNA-binding pseudobarrel domain superfamily	SUPERFAMILY	101936	DNA-binding pseudobarrel domain	coord: 144..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017086.1	MS017086.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003677	DNA binding