MS017006 (gene) Bitter gourd (TR) v1

Overview


Name	MS017006
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cysteine proteinase inhibitor
Location	scaffold197: 599790 .. 599967 (-)
RNA-Seq Expression	MS017006
Synteny	MS017006

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGAATGCTCTTCTGGAATTTGAGCGGGTGTTGAAGCCTAAAACAGCAGGTAGTTTCCGGTATGTCGTATCTTATTACACTGGAAGCCATCGATGGTGGTAAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTGCAGCCGATGAACTTCCAAGAGTTGCAGGAATTCAACGCATGCCCA

mRNA sequence

CAGAATGCTCTTCTGGAATTTGAGCGGGTGTTGAAGAAACAGCAGGTAGTTTCCGGTATGTCGTATCTTATTACACTGGAAGCCATCGATGGTGGTAAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTGCAGCCGATGAACTTCCAAGAGTTGCAGGAATTCAACGCATGCCCA

Coding sequence (CDS)

CAGAATGCTCTTCTGGAATTTGAGCGGGTGTTGAAGAAACAGCAGGTAGTTTCCGGTATGTCGTATCTTATTACACTGGAAGCCATCGATGGTGGTAAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTGCAGCCGATGAACTTCCAAGAGTTGCAGGAATTCAACGCATGCCCA

Protein sequence

QNALLEFERVLKKQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQPMNFQELQEFNACP

Homology

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BLAST of MS017006 vs. NCBI nr
Match: XP_038880981.1 (cysteine proteinase inhibitor A-like [Benincasa hispida])

HSP 1 Score: 88.2 bits (217), Expect = 2.5e-14
Identity = 44/60 (73.33%), Postives = 52/60 (86.67%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNACP 59
           +N LLEFERVLK K+QV++G  Y +TLEAIDGGKKKVYEAKVWV+P MNF++LQEF  CP
Sbjct: 87  ENGLLEFERVLKAKEQVIAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLCP 146

[Close]

BLAST of MS017006 vs. NCBI nr
Match: XP_022932782.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932784.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >KAG7034455.1 hypothetical protein SDJN02_04184 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 85.5 bits (210), Expect = 1.6e-13
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNACP 59
           +N LLEFERVLK K+QVV+G  Y +TLEAIDGG KKVYEAKVWV+P MNF++LQEF  CP
Sbjct: 63  ENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKPCP 122

[Close]

BLAST of MS017006 vs. NCBI nr
Match: XP_023543498.1 (cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543501.1 cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 85.1 bits (209), Expect = 2.2e-13
Identity = 44/60 (73.33%), Postives = 50/60 (83.33%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNACP 59
           QN LLEFERVLK K+QVV+G  Y +TLEA DGG KKVYEAKVWV+P MNF++LQEF  CP
Sbjct: 63  QNGLLEFERVLKAKEQVVAGKLYHLTLEASDGGMKKVYEAKVWVKPWMNFKQLQEFKPCP 122

[Close]

BLAST of MS017006 vs. NCBI nr
Match: XP_008455746.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901819.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >KAA0025834.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa] >TYK09622.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa])

HSP 1 Score: 84.7 bits (208), Expect = 2.8e-13
Identity = 43/59 (72.88%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 58
           +N LLEF+RVLK K+QVV+G  Y +TLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 63  ENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 121

[Close]

BLAST of MS017006 vs. NCBI nr
Match: XP_022967919.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967921.1 cysteine proteinase inhibitor A-like [Cucurbita maxima])

HSP 1 Score: 84.7 bits (208), Expect = 2.8e-13
Identity = 44/59 (74.58%), Postives = 50/59 (84.75%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 58
           +N LLEFERVLK K+QVVSG  Y +TLEAIDGG KKVYEAKVWV+P MNF++LQEF  C
Sbjct: 63  ENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKPC 121

[Close]

BLAST of MS017006 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.2e-13
Identity = 41/56 (73.21%), Postives = 47/56 (83.93%), Query Frame = 0


Query: 1  QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
          QNALLEF RV+  +QQVVSG  Y ITLEA DGG+KKVYEAKVW +P +NF+ELQEF
Sbjct: 34 QNALLEFGRVVSAQQQVVSGTLYTITLEAKDGGQKKVYEAKVWEKPWLNFKELQEF 89

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BLAST of MS017006 vs. ExPASy Swiss-Prot
Match: Q10992 (Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.2e-12
Identity = 38/56 (67.86%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 1  QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQPM-NFQELQEF 55
          QNALLEF +V+  K+QVV+G  Y ITLEA +GG KK YEAKVWV+P  NF+ELQEF
Sbjct: 19 QNALLEFGKVVNTKEQVVAGKMYYITLEATNGGVKKTYEAKVWVKPWENFKELQEF 74

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BLAST of MS017006 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 1.2e-11
Identity = 35/56 (62.50%), Postives = 48/56 (85.71%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           +NALLEF RV+K K+QVV+G  + +TLE ++ G+KK+YEAKVWV+P +NF+ELQEF
Sbjct: 67  ENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEF 122

[Close]

BLAST of MS017006 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.6e-11
Identity = 35/56 (62.50%), Postives = 49/56 (87.50%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           +NALLEF RV++ K+QVV+G  + +TLEA++ G+KKVYEAKVWV+P ++F+ELQEF
Sbjct: 78  ENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLDFKELQEF 133

[Close]

BLAST of MS017006 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 1.6e-11
Identity = 35/56 (62.50%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           +NA+LE  RVLK  +QVV+G  Y +TLE I+ G+KK+YEAKVWV+P MNF++LQEF
Sbjct: 76  ENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEF 131

[Close]

BLAST of MS017006 vs. ExPASy TrEMBL
Match: A0A6J1F2Q3 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.0e-14
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNACP 59
           +N LLEFERVLK K+QVV+G  Y +TLEAIDGG KKVYEAKVWV+P MNF++LQEF  CP
Sbjct: 63  ENGLLEFERVLKAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKPCP 122

[Close]

BLAST of MS017006 vs. ExPASy TrEMBL
Match: A0A6J1HVR4 (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-13
Identity = 44/59 (74.58%), Postives = 50/59 (84.75%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 58
           +N LLEFERVLK K+QVVSG  Y +TLEAIDGG KKVYEAKVWV+P MNF++LQEF  C
Sbjct: 63  ENGLLEFERVLKAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKPC 121

[Close]

BLAST of MS017006 vs. ExPASy TrEMBL
Match: A0A1S4E0R5 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-13
Identity = 43/59 (72.88%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 58
           +N LLEF+RVLK K+QVV+G  Y +TLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 63  ENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 121

[Close]

BLAST of MS017006 vs. ExPASy TrEMBL
Match: A0A5D3CEB5 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00160 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-13
Identity = 43/59 (72.88%), Postives = 51/59 (86.44%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 58
           +N LLEF+RVLK K+QVV+G  Y +TLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 63  ENGLLEFDRVLKAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 121

[Close]

BLAST of MS017006 vs. ExPASy TrEMBL
Match: A0A438J7Q5 (Cysteine proteinase inhibitor OS=Vitis vinifera OX=29760 GN=Os01g0270100_2 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.5e-12
Identity = 42/56 (75.00%), Postives = 49/56 (87.50%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           QNALLEF +VLK K+QVV+G  Y +TLEAID GKKK+YEAKVWV+P MNF+ELQEF
Sbjct: 67  QNALLEFAKVLKAKEQVVAGKIYYLTLEAIDAGKKKIYEAKVWVKPWMNFKELQEF 122

[Close]

BLAST of MS017006 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 69.7 bits (169), Expect = 8.7e-13
Identity = 35/56 (62.50%), Postives = 48/56 (85.71%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           +NALLEF RV+K K+QVV+G  + +TLE ++ G+KK+YEAKVWV+P +NF+ELQEF
Sbjct: 67  ENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEF 122

[Close]

BLAST of MS017006 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 69.7 bits (169), Expect = 8.7e-13
Identity = 35/56 (62.50%), Postives = 48/56 (85.71%), Query Frame = 0


Query: 1  QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
          +NALLEF RV+K K+QVV+G  + +TLE ++ G+KK+YEAKVWV+P +NF+ELQEF
Sbjct: 34 ENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEF 89

[Close]

BLAST of MS017006 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-12
Identity = 35/56 (62.50%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           +NA+LE  RVLK  +QVV+G  Y +TLE I+ G+KK+YEAKVWV+P MNF++LQEF
Sbjct: 76  ENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEF 131

[Close]

BLAST of MS017006 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 62.8 bits (151), Expect = 1.1e-10
Identity = 31/56 (55.36%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 1   QNALLEFERVLK-KQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 55
           QN +LEF++++K ++QVV+G  Y +TLEA +G + K +EAKVWV+P MNF++LQEF
Sbjct: 65  QNKILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEF 120

[Close]

BLAST of MS017006 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 43.9 bits (102), Expect = 5.1e-05
Identity = 25/57 (43.86%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 1  QNALLEFERVL-KKQQVVSGMSYLITLEAIDGGKKKVYEAKVWVQPM-NFQELQEFN 56
          +N  LE++R+L  K QVV+G  + +T+E  DG   KVYEAKV  +   N ++L+ FN
Sbjct: 40 ENLTLEYKRLLGAKTQVVAGTMHHLTVEVADGETNKVYEAKVLEKAWENLKQLESFN 96

The following BLAST results are available for this feature:

BLAST of MS017006 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880981.1	2.5e-14	73.33	cysteine proteinase inhibitor A-like [Benincasa hispida]	[more]
XP_022932782.1	1.6e-13	73.33	cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cystei...	[more]
XP_023543498.1	2.2e-13	73.33	cysteine proteinase inhibitor A-like [Cucurbita pepo subsp. pepo] >XP_023543500....	[more]
XP_008455746.1	2.8e-13	72.88	PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDIC...	[more]
XP_022967919.1	2.8e-13	74.58	cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine...	[more]

BLAST of MS017006 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06445	2.2e-13	73.21	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q10992	4.2e-12	67.86	Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]
Q8H0X6	1.2e-11	62.50	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q0JNR2	1.6e-11	62.50	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q8LC76	1.6e-11	62.50	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]

BLAST of MS017006 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2Q3	8.0e-14	73.33	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3...	[more]
A0A6J1HVR4	1.4e-13	74.58	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 S...	[more]
A0A1S4E0R5	1.4e-13	72.88	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1	[more]
A0A5D3CEB5	1.4e-13	72.88	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A438J7Q5	1.5e-12	75.00	Cysteine proteinase inhibitor OS=Vitis vinifera OX=29760 GN=Os01g0270100_2 PE=3 ...	[more]

BLAST of MS017006 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12490.2	8.7e-13	62.50	cystatin B	[more]
AT3G12490.1	8.7e-13	62.50	cystatin B	[more]
AT5G05110.1	1.1e-12	62.50	Cystatin/monellin family protein	[more]
AT2G40880.1	1.1e-10	55.36	cystatin A	[more]
AT5G12140.1	5.1e-05	43.86	cystatin-1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 3..57 e-value: 1.2E-8 score: 35.2
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 5..55 e-value: 2.48163E-7 score: 41.6908
None	No IPR available	GENE3D	3.10.450.10		coord: 1..58 e-value: 1.0E-19 score: 72.5
None	No IPR available	PANTHER	PTHR11413:SF110	CYSTEINE PROTEINASE INHIBITOR 7	coord: 1..55
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 2..55
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 1..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS017006.1	MS017006.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity