MS016986 (gene) Bitter gourd (TR) v1

Overview
NameMS016986
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsulfoquinovosidase-like isoform X1
Locationscaffold197: 329479 .. 334383 (-)
RNA-Seq ExpressionMS016986
SyntenyMS016986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAACCTTAAAATCACCAAAAAACATCACATTCACTTCAACAACCCTTTCCCTTCAGCTCCAACTTCACTTCCTTCAGTTGAGGGAGATCTCTCTGCAAACTTTCAAGCACTTCCTGCGATCAAAGTGTTCTCAATCGGACAGGATTTTCAGCTTCTATGGAGGTTTGAAAATGGTGGGTCTCTCTCAATCTACCATCTCTCTCAACCCACCAGATCCATCTGGTCAACAATCCCAGGGCAAGCTTTTGTGTCTGCAGCAATGGTGGAAACAGAGGTGGAAGAAAGCCGAGGCTCGTTTGCTGTGAAGGATGGGGCTGTTCATTTGGTCTGTAATCATCAAACAGTTGACGATATCAGGGTAATCAATGGCTGGGATCATGAGTTGGAGGTTAAGGATCATCATTTTCCATCTGGGTATTTGGGATTGGACCAGAAAATGCACCTGAAAGATGCCCAGTTTCCTATGCTGCTCATTAATGGTAGAATATTCAATACAAAGAAGAAGATGACGAGGAGGAAGAAGAAGAACAGGCTTCAAGAAACTGGTTTCAATGGAGATTTAAAGTACAACCCAAGAGCCCCTCCTGCTTCTGCAAGGTATTGGGTATTGTTTGAACAGAAAAACAGCAGCCAAATTGGGTTTCAGGTGATGCTTGGCCAACCCAGCTATGAATGTCGCCAAATGGCTCATTCAAGAGGGCGATTCGACAAGTTTAAGTTTCGATTGCATCGACTTAAGAAACGGAAAGTTGAATGGTATTGGTCTTTGGCAAAACTGAAAGGGTGTGTTAGAGTTTCCTCATCAGAAGAGGAAATGGAAGGCTTGAGATCAGCTGAGGAATTTGAAGGATTCAACAGGGTCTGCTTCACCTACACAAGTGAAGAAAAGGAAAGATTCTTTGGATTTGGAGAGCAGTTTTCTCATATGGATTTCAAGGGGAAAAGAGTTCCAATCTTTGTTCAAGAACAGGGCATTGGTAGAGGAGATCAGCCTATCACTTTTGCAGCTAATCTGGTTAGCTACAGGTAAGTTTAAACAAACAGTACACTTATTCATCTCTTGGTTCTAAAACTGAGTTCAAAACGATCCATTTAAATTTCTGGAACTTACCTTCACTTCAGGGCTGGAGGAGATTGGAGTACTACTTATGCTCCTTCTCCATTTTATATGACATCCAGAATGAGATCTCTGTACCTTGAAGGATATGAATACTCTGTATTTGATCTGACAAAGAATGATAGAGTTCAGATTCAGGTACCCTTCAATAACTATACTATAGCTCTTTTATTGCCTTCCACCAACTTAAAAAACCATGAAATGCTTGAAAAGAGCTTTGGTTTAAGACAAAAGTAGAAAGTAAAAGAAACATAATAATACCAAACAAATAAAGATAGAAGTGAAAAAGCTTAGTGCATTTTACCAACCTAAAGCCATGAAATGTTTGAAAAAAAGCCTTGGTTTAACACAAAAGTAGAAAGTAAATAGATATGAACTCCAAAAGAACATCTCAGGATATCCCCCCAACCCCAAGTAAGGAGCTCATATAAGAATTTTAAACTTGCAGTTTTCCCATTTTCTGTGAACTGGAGATTGCAGATACATGGGAATTCAATTCAAGGATGGATATTGCATGGAAACTCACCTTCTGAGCTTATTGAACGCTTCACCGACACCATTGGGAGGCCTCCAGAGCTTCCAGGATGGATAATATCAGGTGCTGTGGTTGGGATGCAGGGCGGGACTGATGCTGTACGCCAAATCTGGGATGACCTTAAAGTTTATAAAGTTCCCATTTCTGCATTTTGGTTGCAGGTAGTTCAAATTTCTCATTTCTACAAGCTAGTATTAAGATTTCTGGAACAAATTCAACATCCTATACTAATAGATGAAAAATATAATGCAGAACCACAAGTCATACAGTATTTTTCTCATGCTTTTATAGTTGTATAGTTTTTCTAAAGTTTCTGTACATTCTTTTACATTCCTCATCTGAGAAAATAATGTGTAGGACTGGGTGGGGCAGAGAGAAACAGTGATTGGATCACAACTATGGTGGAATTGGGAAGTTGACACAACAAGGTACTGTGGATGGAAACAATTAATTAAAGATCTTGGTGCTCAGCATATCAAAGTGATGACATACTGCAATCCCTGCCTAGCTCCGGTATCTTCTCCTTTCTCACTAAACTCTGTTCTTCTGCAATCACCAAATTGGAAATATTCTTACTACTCTTGCATTACGTTAAAAGGATGGATTCTTTTGAAGTCTTGTCTTCCACCAGATAGACATAGACACTGCTTTCTTAATGCCTGACTAAAATGGAGAGCTTATTATACAATTAGTGGATATGAGCAGAGAAGTGGAGGTTTAATTGTAGACTAATGATTTTCAGTTTCAAATTTCTATATGACTTCAGACTGATGAGAAGCAGAATAGAAGGAGAAACCTTTATGAGGAAGCAAAGGAGTTGGGGATCTTGATAAAGAAGAAGAATGGAGAACCCTACATGGTTCCCAACACAGCTTTTGATGTGGGAATGTTGGATCTTACACACCCAAATACTTCCAGTTGGTTCAAGGAAATTCTGCAAGAAATGGTGGATGATGGGGTGAGAGGATGGATGGCTGATTTTGGCGAAGGCTTGCCTGTAGATGCCACCCTCTATTCAGGTTGTACTAATTTCCCACCTCAGAACGAACATCGATTCTGGCTGCATCCACATTTAACCTCTTTATATATCTGCTTACACCTTCTGTTACTAAGCAAAAGACGACCCTTAACTGCTAATATTTGTACTCAATTTCAAAATAAATACCCAATAATGGTTGATTATCTCACAATTAAGAGATAATTTTCTATTTGTCATCTTGTAGACCATCAGATAAATCCTTTATATTTCGGTTGCTACTTCAAATTTATGCTGTAATCCATCTTACTTGCTTCTTTGAACATTGAGCTACCAACTCGGGTTGAACTTGAAATTTGTAATTCCCACAAGGTTGTCAAATGAGGTCCATATTTCCCTTTTTTCCAACATTTTTATGTCTGTATCCTGGACAAAAAGTTGATTCAAACAAGTGTGATTATCAATGTAACTTATTGTCACTAAAATACTGTTTAGTTTACCTTAATCAGAAATGATTTCACATTTCAGAACTGCTCTGTAATATCACTTACAGATTGACTCATAATTTCAGGTGAAGATCCCATTGCTGCCCATAATAGATACCCAGAAATATGGGCCCAGATGAACCGAGAATTCGCAGATGAATGGAAAAGCAAACTTGTCGGTAAGGAGAAAGAAGACCCACATGAGGCCTTAGTCTTCTTCATGAGAGCTGGTTTCAGAAACAGTCCCAAATGGGGGATGCTATTTTGGGAAGGAGACCAGATGGTAAGTTGGCAAGCCAATGATGGAATAAAGAGTGCTGTCACTGGCCTTCTCAGCAGTGGACTTTCTGGGTATGCTTTTAACCACAGTGATATAGGAGGGTACTGTGCGGTAAATTTACCATTCATCAAGTACCGAAGAAGTGAGGAACTGCTTCTACGGTGGATGGAGTTAAATGCCTTTACCACTATTTTCAGAACTCATGAAGTAAGTTAAAAACCAACTTTACTTTTTTTCCCATTCTGTTCTCTGCTCCTTTCTCTGAGAAGAATCAAAACCTAAAAAAACATTATGTCATGACATCAAATTCATAAGAAACATCTTTCTTTCATAACCACATCCTCAGATTATCCTCACTAAAATGCCCCAAACAAATGGAAACTTGTACCCTGCAGCAGAGTTTTATATATATACAAATAAGGTATGGGGAAAGCCTCATTTTGGACTGAGACTCGAAAATGAAATATGCTACAGCTATATAAATTTTGCATCAGAATATTAGTCACTGTTTTCAACTGTCTTCTGCATGTAGGGAAACAAACCATCCTGCAACAGCCAATTCTACTCAAACGACCGAACTTTGACACAGTTTGCTCGCTTTGCCAAGGTCTATAGTGCTTGGAAGTTTTACAGAGTCCAACTTGTAAAGGTACGTACACAAACTTTTACATTCCAACTGCGACTTCCATATTTGCAAATGGCTAAATTATGTTTTAGTGACTAAAATTTGAGTGCTTTTTGACTTAGTCCCTATTGTTAATAAAGTTTTTTTAGTCATCTCTTATGTTTTTAACATTTTTTTAGTTACATTCTTTCGTCAGTAGATGTTAAATATGGCTAATAGTAAACTTATGCAGCATCATACTTATTGAGTTGGTAGAAATTCAGGAGAGGAATTAAAATTTTCCTCTCTTAGGTAGCCTAGTTCCTCTTCTAAATTTCTTCCAATTCACTAAGAATCATGTCACATGATCTTACTATCAGAAAGGGTATAACCGAAAAATGTTAAACCATAAGGGACTAAATTATAACTTTTTAAACGATGGGAACTAAACCGAAAAAAGCTCAAACATTAGAGACTAAAACATATAAACATATATTTAGCATTTGCAAATTCCTCTGCGGATCCCATGAACTTCTATCTCTTGGGTGGCCGGGTCACAAAACAATACAGGAAGCTGCTCAAAAGGGCCTGCCTGTGTGCCGCCACCTATTTGTTCACTACCCAGAAGATGAGTATGTGCTTACATTGAGTCACCAGCAGTTTTTGGTTGGTTCTGAGATCCTTGTTGTGCCTGTTCTTGACAAAGGCAAGAACAATGTCAAAGCCTACTTTCCTTTGGGAGAAAGTTCTTCATGGCAGCATATATGGACGGGGGAATTATATACTAAACCAGGCTGCGAAGTTAAGGTAGACGCTCCAGTTGGCTATCCTGCTGTATTCATCAAGGTTGGCTCCATTGTCGGGGAAACCTTTGTTAGAAACCTGAAAAATTATAACATTCTT

mRNA sequence

ATGACAAACCTTAAAATCACCAAAAAACATCACATTCACTTCAACAACCCTTTCCCTTCAGCTCCAACTTCACTTCCTTCAGTTGAGGGAGATCTCTCTGCAAACTTTCAAGCACTTCCTGCGATCAAAGTGTTCTCAATCGGACAGGATTTTCAGCTTCTATGGAGGTTTGAAAATGGTGGGTCTCTCTCAATCTACCATCTCTCTCAACCCACCAGATCCATCTGGTCAACAATCCCAGGGCAAGCTTTTGTGTCTGCAGCAATGGTGGAAACAGAGGTGGAAGAAAGCCGAGGCTCGTTTGCTGTGAAGGATGGGGCTGTTCATTTGGTCTGTAATCATCAAACAGTTGACGATATCAGGGTAATCAATGGCTGGGATCATGAGTTGGAGGTTAAGGATCATCATTTTCCATCTGGGTATTTGGGATTGGACCAGAAAATGCACCTGAAAGATGCCCAGTTTCCTATGCTGCTCATTAATGGTAGAATATTCAATACAAAGAAGAAGATGACGAGGAGGAAGAAGAAGAACAGGCTTCAAGAAACTGGTTTCAATGGAGATTTAAAGTACAACCCAAGAGCCCCTCCTGCTTCTGCAAGGTATTGGGTATTGTTTGAACAGAAAAACAGCAGCCAAATTGGGTTTCAGGTGATGCTTGGCCAACCCAGCTATGAATGTCGCCAAATGGCTCATTCAAGAGGGCGATTCGACAAGTTTAAGTTTCGATTGCATCGACTTAAGAAACGGAAAGTTGAATGGTATTGGTCTTTGGCAAAACTGAAAGGGTGTGTTAGAGTTTCCTCATCAGAAGAGGAAATGGAAGGCTTGAGATCAGCTGAGGAATTTGAAGGATTCAACAGGGTCTGCTTCACCTACACAAGTGAAGAAAAGGAAAGATTCTTTGGATTTGGAGAGCAGTTTTCTCATATGGATTTCAAGGGGAAAAGAGTTCCAATCTTTGTTCAAGAACAGGGCATTGGTAGAGGAGATCAGCCTATCACTTTTGCAGCTAATCTGGTTAGCTACAGGGCTGGAGGAGATTGGAGTACTACTTATGCTCCTTCTCCATTTTATATGACATCCAGAATGAGATCTCTGTACCTTGAAGGATATGAATACTCTGTATTTGATCTGACAAAGAATGATAGAGTTCAGATTCAGATACATGGGAATTCAATTCAAGGATGGATATTGCATGGAAACTCACCTTCTGAGCTTATTGAACGCTTCACCGACACCATTGGGAGGCCTCCAGAGCTTCCAGGATGGATAATATCAGGTGCTGTGGTTGGGATGCAGGGCGGGACTGATGCTGTACGCCAAATCTGGGATGACCTTAAAGTTTATAAAGTTCCCATTTCTGCATTTTGGTTGCAGGACTGGGTGGGGCAGAGAGAAACAGTGATTGGATCACAACTATGGTGGAATTGGGAAGTTGACACAACAAGGTACTGTGGATGGAAACAATTAATTAAAGATCTTGGTGCTCAGCATATCAAAGTGATGACATACTGCAATCCCTGCCTAGCTCCGACTGATGAGAAGCAGAATAGAAGGAGAAACCTTTATGAGGAAGCAAAGGAGTTGGGGATCTTGATAAAGAAGAAGAATGGAGAACCCTACATGGTTCCCAACACAGCTTTTGATGTGGGAATGTTGGATCTTACACACCCAAATACTTCCAGTTGGTTCAAGGAAATTCTGCAAGAAATGGTGGATGATGGGGTGAGAGGATGGATGGCTGATTTTGGCGAAGGCTTGCCTGTAGATGCCACCCTCTATTCAGGTTGTGAAGATCCCATTGCTGCCCATAATAGATACCCAGAAATATGGGCCCAGATGAACCGAGAATTCGCAGATGAATGGAAAAGCAAACTTGTCGGTAAGGAGAAAGAAGACCCACATGAGGCCTTAGTCTTCTTCATGAGAGCTGGTTTCAGAAACAGTCCCAAATGGGGGATGCTATTTTGGGAAGGAGACCAGATGGTAAGTTGGCAAGCCAATGATGGAATAAAGAGTGCTGTCACTGGCCTTCTCAGCAGTGGACTTTCTGGGTATGCTTTTAACCACAGTGATATAGGAGGGTACTGTGCGGTAAATTTACCATTCATCAAGTACCGAAGAAGTGAGGAACTGCTTCTACGGTGGATGGAGTTAAATGCCTTTACCACTATTTTCAGAACTCATGAAGGAAACAAACCATCCTGCAACAGCCAATTCTACTCAAACGACCGAACTTTGACACAGTTTGCTCGCTTTGCCAAGGTCTATAGTGCTTGGAAGTTTTACAGAGTCCAACTTGTAAAGGAAGCTGCTCAAAAGGGCCTGCCTGTGTGCCGCCACCTATTTGTTCACTACCCAGAAGATGAGTATGTGCTTACATTGAGTCACCAGCAGTTTTTGGTTGGTTCTGAGATCCTTGTTGTGCCTGTTCTTGACAAAGGCAAGAACAATGTCAAAGCCTACTTTCCTTTGGGAGAAAGTTCTTCATGGCAGCATATATGGACGGGGGAATTATATACTAAACCAGGCTGCGAAGTTAAGGTAGACGCTCCAGTTGGCTATCCTGCTGTATTCATCAAGGTTGGCTCCATTGTCGGGGAAACCTTTGTTAGAAACCTGAAAAATTATAACATTCTT

Coding sequence (CDS)

ATGACAAACCTTAAAATCACCAAAAAACATCACATTCACTTCAACAACCCTTTCCCTTCAGCTCCAACTTCACTTCCTTCAGTTGAGGGAGATCTCTCTGCAAACTTTCAAGCACTTCCTGCGATCAAAGTGTTCTCAATCGGACAGGATTTTCAGCTTCTATGGAGGTTTGAAAATGGTGGGTCTCTCTCAATCTACCATCTCTCTCAACCCACCAGATCCATCTGGTCAACAATCCCAGGGCAAGCTTTTGTGTCTGCAGCAATGGTGGAAACAGAGGTGGAAGAAAGCCGAGGCTCGTTTGCTGTGAAGGATGGGGCTGTTCATTTGGTCTGTAATCATCAAACAGTTGACGATATCAGGGTAATCAATGGCTGGGATCATGAGTTGGAGGTTAAGGATCATCATTTTCCATCTGGGTATTTGGGATTGGACCAGAAAATGCACCTGAAAGATGCCCAGTTTCCTATGCTGCTCATTAATGGTAGAATATTCAATACAAAGAAGAAGATGACGAGGAGGAAGAAGAAGAACAGGCTTCAAGAAACTGGTTTCAATGGAGATTTAAAGTACAACCCAAGAGCCCCTCCTGCTTCTGCAAGGTATTGGGTATTGTTTGAACAGAAAAACAGCAGCCAAATTGGGTTTCAGGTGATGCTTGGCCAACCCAGCTATGAATGTCGCCAAATGGCTCATTCAAGAGGGCGATTCGACAAGTTTAAGTTTCGATTGCATCGACTTAAGAAACGGAAAGTTGAATGGTATTGGTCTTTGGCAAAACTGAAAGGGTGTGTTAGAGTTTCCTCATCAGAAGAGGAAATGGAAGGCTTGAGATCAGCTGAGGAATTTGAAGGATTCAACAGGGTCTGCTTCACCTACACAAGTGAAGAAAAGGAAAGATTCTTTGGATTTGGAGAGCAGTTTTCTCATATGGATTTCAAGGGGAAAAGAGTTCCAATCTTTGTTCAAGAACAGGGCATTGGTAGAGGAGATCAGCCTATCACTTTTGCAGCTAATCTGGTTAGCTACAGGGCTGGAGGAGATTGGAGTACTACTTATGCTCCTTCTCCATTTTATATGACATCCAGAATGAGATCTCTGTACCTTGAAGGATATGAATACTCTGTATTTGATCTGACAAAGAATGATAGAGTTCAGATTCAGATACATGGGAATTCAATTCAAGGATGGATATTGCATGGAAACTCACCTTCTGAGCTTATTGAACGCTTCACCGACACCATTGGGAGGCCTCCAGAGCTTCCAGGATGGATAATATCAGGTGCTGTGGTTGGGATGCAGGGCGGGACTGATGCTGTACGCCAAATCTGGGATGACCTTAAAGTTTATAAAGTTCCCATTTCTGCATTTTGGTTGCAGGACTGGGTGGGGCAGAGAGAAACAGTGATTGGATCACAACTATGGTGGAATTGGGAAGTTGACACAACAAGGTACTGTGGATGGAAACAATTAATTAAAGATCTTGGTGCTCAGCATATCAAAGTGATGACATACTGCAATCCCTGCCTAGCTCCGACTGATGAGAAGCAGAATAGAAGGAGAAACCTTTATGAGGAAGCAAAGGAGTTGGGGATCTTGATAAAGAAGAAGAATGGAGAACCCTACATGGTTCCCAACACAGCTTTTGATGTGGGAATGTTGGATCTTACACACCCAAATACTTCCAGTTGGTTCAAGGAAATTCTGCAAGAAATGGTGGATGATGGGGTGAGAGGATGGATGGCTGATTTTGGCGAAGGCTTGCCTGTAGATGCCACCCTCTATTCAGGTTGTGAAGATCCCATTGCTGCCCATAATAGATACCCAGAAATATGGGCCCAGATGAACCGAGAATTCGCAGATGAATGGAAAAGCAAACTTGTCGGTAAGGAGAAAGAAGACCCACATGAGGCCTTAGTCTTCTTCATGAGAGCTGGTTTCAGAAACAGTCCCAAATGGGGGATGCTATTTTGGGAAGGAGACCAGATGGTAAGTTGGCAAGCCAATGATGGAATAAAGAGTGCTGTCACTGGCCTTCTCAGCAGTGGACTTTCTGGGTATGCTTTTAACCACAGTGATATAGGAGGGTACTGTGCGGTAAATTTACCATTCATCAAGTACCGAAGAAGTGAGGAACTGCTTCTACGGTGGATGGAGTTAAATGCCTTTACCACTATTTTCAGAACTCATGAAGGAAACAAACCATCCTGCAACAGCCAATTCTACTCAAACGACCGAACTTTGACACAGTTTGCTCGCTTTGCCAAGGTCTATAGTGCTTGGAAGTTTTACAGAGTCCAACTTGTAAAGGAAGCTGCTCAAAAGGGCCTGCCTGTGTGCCGCCACCTATTTGTTCACTACCCAGAAGATGAGTATGTGCTTACATTGAGTCACCAGCAGTTTTTGGTTGGTTCTGAGATCCTTGTTGTGCCTGTTCTTGACAAAGGCAAGAACAATGTCAAAGCCTACTTTCCTTTGGGAGAAAGTTCTTCATGGCAGCATATATGGACGGGGGAATTATATACTAAACCAGGCTGCGAAGTTAAGGTAGACGCTCCAGTTGGCTATCCTGCTGTATTCATCAAGGTTGGCTCCATTGTCGGGGAAACCTTTGTTAGAAACCTGAAAAATTATAACATTCTT

Protein sequence

MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENGGSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDIRVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRLQETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKFKFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKERFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYMTSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPELPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCEDPIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGELYTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL
Homology
BLAST of MS016986 vs. NCBI nr
Match: XP_022153908.1 (uncharacterized protein LOC111021314 isoform X1 [Momordica charantia])

HSP 1 Score: 1832.0 bits (4744), Expect = 0.0e+00
Identity = 875/881 (99.32%), Postives = 878/881 (99.66%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKV SIGQDFQLLWRFENG
Sbjct: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVLSIGQDFQLLWRFENG 60

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI
Sbjct: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKM RRKKKNRL
Sbjct: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMMRRKKKNRL 180

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFD+F
Sbjct: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDRF 240

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER
Sbjct: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM
Sbjct: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE
Sbjct: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGW+ISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 421 LPGWMISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE
Sbjct: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPN+SSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSG ED
Sbjct: 541 PYMVPNTAFDVGMLDLTHPNSSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSG-ED 600

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC
Sbjct: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL
Sbjct: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL
Sbjct: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 880

BLAST of MS016986 vs. NCBI nr
Match: XP_038882634.1 (sulfoquinovosidase-like [Benincasa hispida])

HSP 1 Score: 1689.5 bits (4374), Expect = 0.0e+00
Identity = 801/881 (90.92%), Postives = 836/881 (94.89%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           MTNLKITKKHH+H NNPFPS PTS P ++GDLSANFQ LP  K FSIG+DFQLLWR ENG
Sbjct: 52  MTNLKITKKHHMHLNNPFPSPPTSFPLLQGDLSANFQTLPPYKAFSIGRDFQLLWRSENG 111

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSIYHLS PTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQT+DDI
Sbjct: 112 GSLSIYHLSHPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTIDDI 171

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           + INGWDHELEVKDHHFPSGYLGLDQK H KD +FPMLLINGRIFNTKKKM  +KKKNRL
Sbjct: 172 KEINGWDHELEVKDHHFPSGYLGLDQKSHQKDTKFPMLLINGRIFNTKKKMMMKKKKNRL 231

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFNGD+K N +  PASARYWVLFEQKNSSQIGFQVMLGQPSYE RQM HSRG F + 
Sbjct: 232 QETGFNGDIKCNSKVLPASARYWVLFEQKNSSQIGFQVMLGQPSYEYRQMTHSRGGFSRL 291

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRL+KRK EWYWSL KLKG VRVSSSEEEME LR+AEEF  FNRV  TY+SEEKER
Sbjct: 292 KFRLHRLRKRKFEWYWSLPKLKGFVRVSSSEEEMEVLRAAEEFGEFNRVFLTYSSEEKER 351

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM
Sbjct: 352 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 411

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TS+MRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQG ILHGNSPSELIERFT+TIGRPPE
Sbjct: 412 TSKMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGRILHGNSPSELIERFTETIGRPPE 471

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGWIISGAVVGMQGGTDAVR+IW+DLK ++VPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 472 LPGWIISGAVVGMQGGTDAVRKIWNDLKAHEVPISAFWLQDWVGQRETVIGSQLWWNWEV 531

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRY GWKQLIKDLGA HIKVMTYCNPCLAPTDEKQNRRRNLYEEAK LGIL+KKKNGE
Sbjct: 532 DTTRYFGWKQLIKDLGAWHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKALGILVKKKNGE 591

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLP+DATLYSG +D
Sbjct: 592 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPIDATLYSG-DD 651

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PI AHNRYPEIWAQ+NREFADEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 652 PITAHNRYPEIWAQINREFADEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWEG 711

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 712 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 771

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTT+FRTHEGNKPSCNSQFYSNDRTL+QFARFAKVYSAWKFYR+QLVKEAAQ+GLP+C
Sbjct: 772 NAFTTVFRTHEGNKPSCNSQFYSNDRTLSQFARFAKVYSAWKFYRIQLVKEAAQRGLPIC 831

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNVKAYFPL ESSSWQHIWTGE+
Sbjct: 832 RHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVKAYFPLNESSSWQHIWTGEV 891

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           + KPGCE+KVDAPVGYPAVFIKVGS+VGETF+RNLK +NIL
Sbjct: 892 HAKPGCEIKVDAPVGYPAVFIKVGSVVGETFIRNLKIFNIL 931

BLAST of MS016986 vs. NCBI nr
Match: KAG6603308.1 (hypothetical protein SDJN03_03917, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1646.3 bits (4262), Expect = 0.0e+00
Identity = 786/881 (89.22%), Postives = 826/881 (93.76%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           M NLKITKKHHIH NNPFPS PTS P V+GDLSANFQALP  +VFSIGQDFQLLWR ENG
Sbjct: 1   MKNLKITKKHHIHLNNPFPSPPTSSPLVQGDLSANFQALPPYRVFSIGQDFQLLWRSENG 60

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSI+HLSQPTRSIWSTIPG+AFVSAAMVETEVEESRGSFAVKDGA+ LVCNHQT+DDI
Sbjct: 61  GSLSIHHLSQPTRSIWSTIPGRAFVSAAMVETEVEESRGSFAVKDGAIRLVCNHQTIDDI 120

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           + ING D+ELEV+DHHFPSGYL LDQK H KDAQFPMLLINGRIFNTKKKM ++K  N L
Sbjct: 121 KEINGCDYELEVRDHHFPSGYLDLDQKSHKKDAQFPMLLINGRIFNTKKKMIKKKNNNSL 180

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFN         PPASARYWVLFEQKNSSQIGFQVMLGQPSYE     HSRGR ++ 
Sbjct: 181 QETGFN--------VPPASARYWVLFEQKNSSQIGFQVMLGQPSYE----YHSRGRLNRL 240

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRL+K KVEW+WSLAKLKGCVRVSSSEEE E L++ EEFE FNRVC TY+SEEKER
Sbjct: 241 KFRLHRLRKGKVEWHWSLAKLKGCVRVSSSEEEREDLKAPEEFEAFNRVCLTYSSEEKER 300

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPI VQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY+
Sbjct: 301 FFGFGEQFSHMDFKGKRVPILVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYI 360

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSRMRSLYLEGYEYSVFDLTKND VQIQI+GNSIQG ILHGNSPSELIERFT+TIGRPPE
Sbjct: 361 TSRMRSLYLEGYEYSVFDLTKNDMVQIQIYGNSIQGRILHGNSPSELIERFTETIGRPPE 420

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGWIISGAVVGMQGGTDAVRQIWD LK ++VPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 421 LPGWIISGAVVGMQGGTDAVRQIWDVLKAHEVPISAFWLQDWVGQRETVIGSQLWWNWEV 480

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDL AQHIKVMTYCNPCLAP D+KQNRRRNLYEEAKELGIL+ KKNGE
Sbjct: 481 DTTRYCGWKQLIKDLDAQHIKVMTYCNPCLAPIDQKQNRRRNLYEEAKELGILV-KKNGE 540

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMV+DGVRGWMADFGEGLP+DATLYSG ED
Sbjct: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVNDGVRGWMADFGEGLPIDATLYSG-ED 600

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PI AHNRYPE+WAQ+NREFADEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 601 PITAHNRYPEMWAQINREFADEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWEG 660

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTT+FRTHEGNKP+CNSQFYSNDRTL+QFARFAKVY AWKFYR+QLVKEAAQ+GLPVC
Sbjct: 721 NAFTTVFRTHEGNKPTCNSQFYSNDRTLSQFARFAKVYCAWKFYRIQLVKEAAQRGLPVC 780

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE+
Sbjct: 781 RHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEI 840

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           Y KPGCE+KVDAPV YPAVFIKVGS+VGETF+RNLK++NIL
Sbjct: 841 YAKPGCEIKVDAPVAYPAVFIKVGSVVGETFIRNLKSFNIL 867

BLAST of MS016986 vs. NCBI nr
Match: XP_022932849.1 (uncharacterized protein LOC111439390 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1638.6 bits (4242), Expect = 0.0e+00
Identity = 784/881 (88.99%), Postives = 824/881 (93.53%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           M NLKITKKHHIH NNPFPS PTS P V+GDLSANFQALP  +VFSIGQDFQLLWR ENG
Sbjct: 1   MKNLKITKKHHIHLNNPFPSPPTSSPLVQGDLSANFQALPPYRVFSIGQDFQLLWRSENG 60

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSI+HLSQPTRSIWSTIPG+AFVSAAMVETEVEESRGSFAVKDGA+ LVCNHQT+DDI
Sbjct: 61  GSLSIHHLSQPTRSIWSTIPGRAFVSAAMVETEVEESRGSFAVKDGAIRLVCNHQTIDDI 120

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           + ING D+ELEV+DHHFPSGYL LDQK H KDAQFPMLLINGRIFNTKKKM ++K  N L
Sbjct: 121 KEINGCDYELEVRDHHFPSGYLDLDQKSHKKDAQFPMLLINGRIFNTKKKMIKKKNNNSL 180

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFN         PPASARYWVLFEQKNSSQIGFQVMLGQPSYE     HSRGR ++ 
Sbjct: 181 QETGFN--------VPPASARYWVLFEQKNSSQIGFQVMLGQPSYE----YHSRGRLNRL 240

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRL+K KVEW+ SLAKLKGCVRVSSSEEE E L++ EEFE FNRVC TY+SEEKER
Sbjct: 241 KFRLHRLRKGKVEWHCSLAKLKGCVRVSSSEEEREDLKAPEEFEAFNRVCLTYSSEEKER 300

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPI VQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY+
Sbjct: 301 FFGFGEQFSHMDFKGKRVPILVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYI 360

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSRMRSLYLEGYEYSVFDLTKND VQIQI+GNSIQG ILHGNSPSELIERFT+TIGRPPE
Sbjct: 361 TSRMRSLYLEGYEYSVFDLTKNDMVQIQIYGNSIQGRILHGNSPSELIERFTETIGRPPE 420

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGWIISGAVVGMQGGTDAVRQIWD LK ++VPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 421 LPGWIISGAVVGMQGGTDAVRQIWDVLKAHEVPISAFWLQDWVGQRETVIGSQLWWNWEV 480

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDL AQHIKVMTYCNPCLAP D+KQNRRRNLYEEAK LGIL+ KKNGE
Sbjct: 481 DTTRYCGWKQLIKDLDAQHIKVMTYCNPCLAPIDQKQNRRRNLYEEAKGLGILV-KKNGE 540

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMV+DGVRGWMADFGEGLP+DATLYSG ED
Sbjct: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVNDGVRGWMADFGEGLPIDATLYSG-ED 600

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PI AHNRYPE+WAQ+NREFADEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 601 PITAHNRYPEMWAQINREFADEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWEG 660

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTT+FRTHEGNKP+CNSQFYSNDRTL+QFARFAKVY AWKFYR+QLVKEAAQ+GLPVC
Sbjct: 721 NAFTTVFRTHEGNKPTCNSQFYSNDRTLSQFARFAKVYCAWKFYRIQLVKEAAQRGLPVC 780

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE+
Sbjct: 781 RHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEI 840

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           Y KPGCE+KVDAPV YPAVFIKVGS+VGETF+RNLK++NIL
Sbjct: 841 YAKPGCEIKVDAPVAYPAVFIKVGSVVGETFIRNLKSFNIL 867

BLAST of MS016986 vs. NCBI nr
Match: XP_022967711.1 (uncharacterized protein LOC111467157 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1637.9 bits (4240), Expect = 0.0e+00
Identity = 780/881 (88.54%), Postives = 826/881 (93.76%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           M NLKITKKHHIH NNPFPSAPTS P V+GDLSANFQALP  +VFSIGQDFQLLWR ENG
Sbjct: 13  MKNLKITKKHHIHLNNPFPSAPTSFPLVQGDLSANFQALPPYRVFSIGQDFQLLWRSENG 72

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSI+HLSQPTRSIWSTIPG+AFVSAAMVETEVEESRGSF+VKDGA+ LVCNHQT+DDI
Sbjct: 73  GSLSIHHLSQPTRSIWSTIPGRAFVSAAMVETEVEESRGSFSVKDGAIRLVCNHQTIDDI 132

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           + ING D+ELEV+DHHFPSGYL LDQK H KDAQFPMLLINGRIFNTKKKM ++KK N L
Sbjct: 133 KEINGCDYELEVRDHHFPSGYLDLDQKSHKKDAQFPMLLINGRIFNTKKKMIKKKKNNSL 192

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFN         PPASARYWVLFEQKN+SQIGFQVMLGQPSYE     HSRGR ++ 
Sbjct: 193 QETGFN--------VPPASARYWVLFEQKNNSQIGFQVMLGQPSYE----YHSRGRLNRL 252

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRL+K KVEW+WSLAKLK CVRVSSSEEE E L++ EEF+ FNRVC +Y+SEEKER
Sbjct: 253 KFRLHRLRKGKVEWHWSLAKLKRCVRVSSSEEEREDLKAPEEFDAFNRVCLSYSSEEKER 312

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPI VQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY+
Sbjct: 313 FFGFGEQFSHMDFKGKRVPILVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYI 372

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSR+RSLYLEGYEYSVFDLTKN+ VQIQI+GNSIQG ILHGNSPSELIERFT+TIGRPPE
Sbjct: 373 TSRLRSLYLEGYEYSVFDLTKNNMVQIQIYGNSIQGRILHGNSPSELIERFTETIGRPPE 432

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGWIISGAVVGMQGGTDAVRQIWD LK ++VPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 433 LPGWIISGAVVGMQGGTDAVRQIWDVLKAHEVPISAFWLQDWVGQRETVIGSQLWWNWEV 492

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDL AQHIKVMTYCNPCLAP D+KQNRRRNLY+EAKELGIL+ KKNGE
Sbjct: 493 DTTRYCGWKQLIKDLDAQHIKVMTYCNPCLAPIDQKQNRRRNLYQEAKELGILV-KKNGE 552

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMV+DGVRGWMADFGEGLP+DATLYSG ED
Sbjct: 553 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVNDGVRGWMADFGEGLPIDATLYSG-ED 612

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PI AHNRYPE+WAQ+NREFADEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 613 PITAHNRYPEMWAQINREFADEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWEG 672

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 673 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 732

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTT+FRTHEGNKP+CNSQFYSNDRTL+QFARFAKVY AWKFYR+QLVKEAAQ+GLPVC
Sbjct: 733 NAFTTVFRTHEGNKPTCNSQFYSNDRTLSQFARFAKVYYAWKFYRIQLVKEAAQRGLPVC 792

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE+
Sbjct: 793 RHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEI 852

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           Y KPGCE+ VDAPV YPAVFIKVGS+VGETF+RNLK+YNIL
Sbjct: 853 YAKPGCEINVDAPVAYPAVFIKVGSVVGETFIRNLKSYNIL 879

BLAST of MS016986 vs. ExPASy Swiss-Prot
Match: P32138 (Sulfoquinovosidase OS=Escherichia coli (strain K12) OX=83333 GN=yihQ PE=1 SV=3)

HSP 1 Score: 397.9 bits (1021), Expect = 3.0e-109
Identity = 212/581 (36.49%), Postives = 328/581 (56.45%), Query Frame = 0

Query: 287 NRVCFTYTSEEKERFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQP-ITFAANLVSYRA 346
           NR+     ++ ++  +G GEQFS+ D +GK  P++  EQG+GR  Q  +T+ A+     A
Sbjct: 115 NRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQAD-CKENA 174

Query: 347 GGDWSTTYAPSPFYMTSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPS 406
           GGD+  T+ P P +++++    +++   Y  FD +  +  ++ +  +         ++  
Sbjct: 175 GGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEDKATLRFECADTYI 234

Query: 407 ELIERFTDTIGRPPELPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQ 466
            L+E+ T  +GR PELP WI  G  +G+QGGT+  ++  D ++   V ++  W QDW G 
Sbjct: 235 SLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGI 294

Query: 467 RETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYE 526
           R T  G ++ WNW+ ++  Y      IK    + ++ + Y NP +A         ++L E
Sbjct: 295 RMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYINPYVASD-------KDLCE 354

Query: 527 EAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSWFKEILQE-MVDDGVRGWMADF 586
           EA + G L K  +G  Y+V    F  G++DLT+P   +WFKE++++ M++ G  GWMADF
Sbjct: 355 EAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCGGWMADF 414

Query: 587 GEGLPVDATLYSGCEDPIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMR 646
           GE LP D  L++G    I  HN +P +WA+ N E  +E      GK  E     ++FFMR
Sbjct: 415 GEYLPTDTYLHNGVSAEI-MHNAWPALWAKCNYEALEE-----TGKLGE-----ILFFMR 474

Query: 647 AGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPF 706
           AG   S K+  + W GDQ V W  +DG+ S V   LS  ++G+  +HSDIGGY  +    
Sbjct: 475 AGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTTL---- 534

Query: 707 IKYRRSEELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFY 766
            + +RS+ELLLRW + +AFT + RTHEGN+P  N QF  +  T+  FAR   V++  K Y
Sbjct: 535 FEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFTTLKPY 594

Query: 767 RVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYF 826
             + V   A+ GLPV R LF+HY +D +  TL + Q+L+G +ILV PV ++G+++   Y 
Sbjct: 595 LKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKY-QYLLGRDILVAPVHEEGRSDWTLYL 654

Query: 827 PLGESSSWQHIWTGELYTKPGCEVKVDAPVGYPAVFIKVGS 866
           P     +W H WTGE +   G EV V+AP+G P VF +  S
Sbjct: 655 P---EDNWVHAWTGEAFR--GGEVTVNAPIGKPPVFYRADS 666

BLAST of MS016986 vs. ExPASy Swiss-Prot
Match: Q9F234 (Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 2.1e-38
Identity = 145/603 (24.05%), Postives = 241/603 (39.97%), Query Frame = 0

Query: 284 EGFNRVCFTYTSE--------EKERFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPIT 343
           EG   + FTY  E        E + F+GFGE+   +D +G+ + ++  +           
Sbjct: 124 EGKKGMAFTYQGEVCCFKMMDEADHFYGFGEKTGFLDKRGETMTMWNTD----------- 183

Query: 344 FAANLVSYRAGGDWSTTYAPSPFYMTSRMRS---LYLEGYEYSVFDL-TKNDRVQIQIHG 403
                V      +    Y   P++MT R  S   ++ +    + FD  T  D       G
Sbjct: 184 -----VYAPHNPETDPLYQSHPYFMTVRNGSAHGIFFDNTYKTTFDFQTATDEYCFSAEG 243

Query: 404 NSIQGWILHGNSPSELIERFTDTIGRPPELPGWIISGAVVGMQGGTD-AVRQIWDDLKVY 463
            +I  ++  G +P +++E++TD  GR P  P W +          T+  VR+I       
Sbjct: 244 GAIDYYVFAGPTPKDVLEQYTDLTGRMPLPPKWALGYHQSRYSYETEQEVREIAQTFIEK 303

Query: 464 KVPISAFWLQDWVGQRETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCL 523
            +P+   +L         V        +  D  R+   KQLI DL  + I+V+   +P +
Sbjct: 304 DIPLDVIYLDIHYMNGYRV--------FTFDRNRFPNLKQLIADLKQKGIRVVPIVDPGV 363

Query: 524 APTDEKQNRRRNLYEEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSWFKEILQ 583
                K++    +Y+E        K   G  Y            D T+     W+ E  Q
Sbjct: 364 -----KEDPEYVIYQEGIRHDYFCKYIEGNVYFGEVWPGKSAFPDFTNKKVRKWWGEKHQ 423

Query: 584 EMVDDGVRGWMAD------FGEGLPVDATLYSGCEDPIAAHNRYPEIWAQMNREFADEWK 643
              D G+ G   D      F E   +D  +    +     H     ++  M  E   +  
Sbjct: 424 FYTDLGIEGIWNDMNEPSVFNETKTMDVKVIHDNDGDPKTHRELHNVYGFMMGEATYKGM 483

Query: 644 SKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGL 703
            KL+  ++           RAGF    ++  + W GD    W   + ++ ++   ++ GL
Sbjct: 484 KKLLNGKRP------FLLTRAGFSGIQRYAAV-WTGDNRSFW---EHLQMSLPMCMNLGL 543

Query: 704 SGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSN 763
           SG AF   D+GG+         +  + ELL RWM++ AFT  FR H          +   
Sbjct: 544 SGVAFCGPDVGGFA--------HNTNGELLTRWMQVGAFTPYFRNHCAIGFRRQEPWAFG 603

Query: 764 DRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVG 823
           ++      ++ ++   W  +   L  EA + G PV R LF  YP+DE    L + +FLVG
Sbjct: 604 EKYERIIKKYIRLRYQWLPHLYTLFAEAHETGAPVMRPLFFEYPDDENTYNL-YDEFLVG 663

Query: 824 SEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGELYTKPGCEVKVDAPVGYPAVFIKVG 868
           + +L+ P++        AYFP G   +W   WTGE+  + G    + A +    +FIK G
Sbjct: 664 ANVLIAPIMTPSTTRRVAYFPKG---NWVDYWTGEV-LEGGQYHLISADLETLPIFIKQG 674

BLAST of MS016986 vs. ExPASy Swiss-Prot
Match: P96793 (Alpha-xylosidase XylQ OS=Lactiplantibacillus pentosus OX=1589 GN=xylQ PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 1.4e-34
Identity = 134/554 (24.19%), Postives = 245/554 (44.22%), Query Frame = 0

Query: 299 ERFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPF 358
           E+ +G GE+F++    G+ V  + Q+ G G                        Y   PF
Sbjct: 158 EKIYGLGERFTNFVKNGQVVDTWNQDGGTGS--------------------EQAYKNIPF 217

Query: 359 YMTSRMRSLYLEGYEYSVFDLTKN--DRVQIQIHGNSIQGWILHGNSPSELIERFTDTIG 418
           Y++S    ++++  +   F++     DRVQ    G S+Q ++++G +P E++ R+T   G
Sbjct: 218 YISSNGYGVFVDESQRVSFEIGSENVDRVQFSTEGQSLQYYVIYGPTPKEVLHRYTQLTG 277

Query: 419 RPPELPGWIISGAVVGMQGGTD----AVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGS 478
                P W   G  +     TD     V +  D ++ + +P+  F   D   Q+    G 
Sbjct: 278 AIKLPPAWSF-GLWLTTSFTTDYSEETVLKFIDGMQEHHIPLDVFHF-DCFWQK----GF 337

Query: 479 QLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGI 538
           + W   E D  ++   + L+K +  + IKV  + NP +A       ++  L++EAK+ G 
Sbjct: 338 E-WCTLEWDKEQFPDPEGLLKKIHDRGIKVCVWLNPYIA-------QKSPLFKEAKDKGY 397

Query: 539 LIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDA 598
           L+ ++NG+ +         G +D T+P    W+++ L+ ++D GV  +  DFGE +P + 
Sbjct: 398 LLTRENGDIWQWDLWQAGNGFVDFTNPAAVKWYQDKLKVLLDMGVDSFKTDFGERIPAED 457

Query: 599 TLYSGCEDPIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPK 658
             +    +P   HN Y     Q NR   +      V ++++   EA++F       ++P 
Sbjct: 458 VKFFDGSNPQQEHNYYT---LQYNRAVYE------VIQQEKGADEAVLFARSQRLVHNP- 517

Query: 659 WGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGY-CAVNLPFIKYRRSE 718
              + + G   +S ++       + G LS  LSG+ F   DIGG+      P      + 
Sbjct: 518 ---IQYTGAATIS-RSTAQCVIQLRGGLSFLLSGFGFWSHDIGGFEDGPGTP------TA 577

Query: 719 ELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKE 778
           +L  RW +    ++  R H  +       F  +D  +    ++    S   +   +    
Sbjct: 578 DLYKRWSQFGLLSSHSRYHGSDVYRVPWNF--DDEAVENTRKYVNKLSLMPYIYTEAAHA 637

Query: 779 AAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVL-DKGKNNVKAYFPLGESS 838
           AA  G P+ R +F+ + +D+ V   +  Q++ GS+ILV P+  D+GK +   Y P G+  
Sbjct: 638 AAAYGNPLMRPMFLEFGDDDNVYD-NATQYMFGSKILVAPIFNDQGKAHF--YLPSGK-- 649

Query: 839 SWQHIWTGELYTKP 845
            W  I  G++Y  P
Sbjct: 698 -WTSILDGKVYQAP 649

BLAST of MS016986 vs. ExPASy Swiss-Prot
Match: B3PEE6 (Oligosaccharide 4-alpha-D-glucosyltransferase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=agd31B PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.6e-33
Identity = 130/586 (22.18%), Postives = 218/586 (37.20%), Query Frame = 0

Query: 287 NRVCFTYTSEEKERFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAG 346
           +++ F +     E+  G G++   MD +G+R P++                 N   Y   
Sbjct: 140 DKINFRFYLSPGEKILGGGQRILGMDRRGQRFPLY-----------------NRAHYGYS 199

Query: 347 GDWSTTYAPSPFYMTSRMRSLYLEGYEYSVFDL--TKNDRVQIQIHGNSIQGWILHGNSP 406
                 Y   P  M+S+   L  +       D+  T++D +Q++         ++ GNS 
Sbjct: 200 DHSGQMYFGLPAIMSSKQYILVFDNSASGAMDIGKTESDILQLEAKSGRSAYILVAGNSY 259

Query: 407 SELIERFTDTIGRPPELPGWIISGAVVGMQGGTDA-VRQIWDDLKVYKVPISAFWLQ-DW 466
             LIE FT   GR P  P W +          ++A  R      K    P+    L   W
Sbjct: 260 PSLIENFTQVTGRQPLPPRWALGSFASRFGYRSEAETRATVQKYKTEDFPLDTIVLDLYW 319

Query: 467 VGQRETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRN 526
            G+        L W+ E   T       ++ D   Q +K +    P +  + ++      
Sbjct: 320 FGKDIKGHMGNLDWDKENFPTPL----DMMADFKQQGVKTVLITEPFVLTSSKR------ 379

Query: 527 LYEEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMA 586
            +++A +   L K   G+P        + G++D+     S WF  I +++   GV GW  
Sbjct: 380 -WDDAVKAKALAKDPQGQPKAFELYFGNGGIIDVFSKEGSRWFSSIYKDLSKQGVAGWWG 439

Query: 587 DFGEGLPVDATLYSGCEDPIAAHNRYPEIWAQM-NREFADEWKSKLVGKEKEDPHEALVF 646
           D GE             D    HN Y   WA+M  ++  D++           P      
Sbjct: 440 DLGEPEMHPEDTQHAIGDADTVHNAYGHRWAEMLYQQQLDQF-----------PELRPFI 499

Query: 647 FMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVN 706
            MRAGF  S ++GM+ W GD   +W    G+ S V   L   L G+ + HSD+GG+    
Sbjct: 500 MMRAGFVGSQRYGMIPWTGDVSRTW---GGLASQVELALQMSLLGFGYIHSDLGGFADGE 559

Query: 707 LPFIKYRRSEELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAW 766
                    +E+ +RW++   F  ++R H G     +   + ++ T        K+    
Sbjct: 560 ------TLDKEMYIRWLQYGVFQPVYRPH-GQDHIPSEPVFQDEETKAILRPLVKLRYRM 619

Query: 767 KFYRVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVK 826
             Y      +    G+P+ R LF    E    L  +   +  G  +LV P+   G  +V 
Sbjct: 620 LPYIYTAAYQNTLTGMPLMRPLFFS-DEKNPALIDNKTSYFWGDSLLVTPITQAGVESVS 672

Query: 827 AYFPLGESSSWQHIWTGELYTKPGCEVKVDAPVGYPAVFIKVGSIV 868
              P G    W   W    Y   G  + +   +    V +K G+ +
Sbjct: 680 IPAPKG---VWFDFWKDTRYQTDGAPLTLPTDLHTIPVLVKAGAFM 672

BLAST of MS016986 vs. ExPASy Swiss-Prot
Match: D2PPM7 (1,3-alpha-isomaltosidase OS=Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399) OX=479435 GN=Kfla_1895 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 7.3e-31
Identity = 159/663 (23.98%), Postives = 261/663 (39.37%), Query Frame = 0

Query: 239 KFKFRLHR-LKKRKVEWYW---SLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYT 298
           K++F  HR       EW+    ++    G   V    E + G+      +G +R  F   
Sbjct: 100 KYRFHAHRGGAAESTEWFEVSPAVWTADGVGEVRGGGERVRGVEWLVSSQGVHRGRFRLQ 159

Query: 299 SEEKERFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYA 358
            ++ +R  GFGE++  +D +G+ +   V EQ                 Y+A G    TY 
Sbjct: 160 LQDGDRLVGFGERYDALDQRGRELDAVVFEQ-----------------YKAQGVHGRTYL 219

Query: 359 PSPFYMT---------SRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPS 418
           P PF              +R+     Y  +  +LT    V++ +    +    ++   P+
Sbjct: 220 PMPFAHVVGADGNGWGFHVRTSRRTWYSSAGNELT----VEVALGDEPVVDLAIYEGDPA 279

Query: 419 ELIERFTDTIGRPPELPGWIISGAVVGMQGGT-DAVRQIWDDLKVYKVPISAFWLQDWVG 478
            ++  F D +GR  ELPGW+      G +  T   V    D  +   +P+ A  ++ W  
Sbjct: 280 TVLTGFLDEVGRAEELPGWVFRLWASGNEWNTQQLVTARMDTHRDLAIPVGAVVIEAWSD 339

Query: 479 QRETVIGSQLWWNWEVDTTRY----------CGW---KQLIKDLGAQHIKVMTYCNPCLA 538
           ++   I     +    D + +            W   K +I +L A+ IKV+ +  P L 
Sbjct: 340 EQGITIWRDAVYAVTEDGSAHRAEDFSYRPDGAWPDPKAMIDELHARGIKVILWQIP-LQ 399

Query: 539 PTDEKQNRRRNLYEEAKELGILIKKKNGEPYMVPNTAFDVGML-DLTHPNTSSWFKEILQ 598
            T+    +           G  + + +G  Y      F   ++ DL+   T  W+ E  +
Sbjct: 400 KTEFSTGQVAADAAAMVRDGHAVLEADGTAYRNRGWWFPQALMPDLSVQRTRDWWTEKRR 459

Query: 599 EMVDD-GVRGWMADFGEGLPVDATLYSGCEDPIAAHNRYPEIWAQMNREFADEWKSKLVG 658
            +V+   V G+  D GE       +Y+        +N YP  +A   R F D  +S   G
Sbjct: 460 YLVEHFDVDGFKTDGGEHAWGHDLVYADGRKGDEGNNLYPVHYA---RAFGDLLRS--AG 519

Query: 659 KEKEDPHEALVFFMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAF 718
           K       A V F RAGF  S   G +FW GD+  +WQA    +S+VT  L++   G  +
Sbjct: 520 K-------APVTFSRAGFTGSQAHG-IFWAGDEDSTWQA---FRSSVTAGLTAASCGIVY 579

Query: 719 NHSDIGGYCAVNLPFIKYRRSEELLLRWMELNAFTTIFRTH----------EGNKPSCNS 778
              D+ G+    +P        EL LR    +AF  I + H              P   +
Sbjct: 580 WGWDLAGFSG-PVP------DAELYLRAAAASAFMPIMQYHSEFNHHQLPLRDRTPWHVA 639

Query: 779 QFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTLSHQ 838
           +   +DR +  F RFA +  +   Y  +          P+ R LF  +  D  +    + 
Sbjct: 640 ETTGDDRVVPLFRRFATLRESLVPYLTEQAARTIATDRPLMRPLFFDHENDPEIWNHPY- 699

Query: 839 QFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGELYTKPGCEVKVDAPVGYPAV 863
           Q+L+G E+L+ PVL+ G      Y P GE   W  +WTG+    P   V  D P+    V
Sbjct: 700 QYLLGDELLINPVLEPGATTWTTYLPAGE---WIDVWTGD--RVPSGLVTRDVPLEVVPV 711

BLAST of MS016986 vs. ExPASy TrEMBL
Match: A0A6J1DIT6 (uncharacterized protein LOC111021314 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021314 PE=3 SV=1)

HSP 1 Score: 1832.0 bits (4744), Expect = 0.0e+00
Identity = 875/881 (99.32%), Postives = 878/881 (99.66%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKV SIGQDFQLLWRFENG
Sbjct: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVLSIGQDFQLLWRFENG 60

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI
Sbjct: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKM RRKKKNRL
Sbjct: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMMRRKKKNRL 180

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFD+F
Sbjct: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDRF 240

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER
Sbjct: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM
Sbjct: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE
Sbjct: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGW+ISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 421 LPGWMISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE
Sbjct: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPN+SSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSG ED
Sbjct: 541 PYMVPNTAFDVGMLDLTHPNSSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSG-ED 600

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC
Sbjct: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL
Sbjct: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL
Sbjct: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 880

BLAST of MS016986 vs. ExPASy TrEMBL
Match: A0A6J1EY61 (uncharacterized protein LOC111439390 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439390 PE=3 SV=1)

HSP 1 Score: 1638.6 bits (4242), Expect = 0.0e+00
Identity = 784/881 (88.99%), Postives = 824/881 (93.53%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           M NLKITKKHHIH NNPFPS PTS P V+GDLSANFQALP  +VFSIGQDFQLLWR ENG
Sbjct: 1   MKNLKITKKHHIHLNNPFPSPPTSSPLVQGDLSANFQALPPYRVFSIGQDFQLLWRSENG 60

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSI+HLSQPTRSIWSTIPG+AFVSAAMVETEVEESRGSFAVKDGA+ LVCNHQT+DDI
Sbjct: 61  GSLSIHHLSQPTRSIWSTIPGRAFVSAAMVETEVEESRGSFAVKDGAIRLVCNHQTIDDI 120

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           + ING D+ELEV+DHHFPSGYL LDQK H KDAQFPMLLINGRIFNTKKKM ++K  N L
Sbjct: 121 KEINGCDYELEVRDHHFPSGYLDLDQKSHKKDAQFPMLLINGRIFNTKKKMIKKKNNNSL 180

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFN         PPASARYWVLFEQKNSSQIGFQVMLGQPSYE     HSRGR ++ 
Sbjct: 181 QETGFN--------VPPASARYWVLFEQKNSSQIGFQVMLGQPSYE----YHSRGRLNRL 240

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRL+K KVEW+ SLAKLKGCVRVSSSEEE E L++ EEFE FNRVC TY+SEEKER
Sbjct: 241 KFRLHRLRKGKVEWHCSLAKLKGCVRVSSSEEEREDLKAPEEFEAFNRVCLTYSSEEKER 300

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPI VQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY+
Sbjct: 301 FFGFGEQFSHMDFKGKRVPILVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYI 360

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSRMRSLYLEGYEYSVFDLTKND VQIQI+GNSIQG ILHGNSPSELIERFT+TIGRPPE
Sbjct: 361 TSRMRSLYLEGYEYSVFDLTKNDMVQIQIYGNSIQGRILHGNSPSELIERFTETIGRPPE 420

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGWIISGAVVGMQGGTDAVRQIWD LK ++VPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 421 LPGWIISGAVVGMQGGTDAVRQIWDVLKAHEVPISAFWLQDWVGQRETVIGSQLWWNWEV 480

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDL AQHIKVMTYCNPCLAP D+KQNRRRNLYEEAK LGIL+ KKNGE
Sbjct: 481 DTTRYCGWKQLIKDLDAQHIKVMTYCNPCLAPIDQKQNRRRNLYEEAKGLGILV-KKNGE 540

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMV+DGVRGWMADFGEGLP+DATLYSG ED
Sbjct: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVNDGVRGWMADFGEGLPIDATLYSG-ED 600

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PI AHNRYPE+WAQ+NREFADEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 601 PITAHNRYPEMWAQINREFADEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWEG 660

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTT+FRTHEGNKP+CNSQFYSNDRTL+QFARFAKVY AWKFYR+QLVKEAAQ+GLPVC
Sbjct: 721 NAFTTVFRTHEGNKPTCNSQFYSNDRTLSQFARFAKVYCAWKFYRIQLVKEAAQRGLPVC 780

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE+
Sbjct: 781 RHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEI 840

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           Y KPGCE+KVDAPV YPAVFIKVGS+VGETF+RNLK++NIL
Sbjct: 841 YAKPGCEIKVDAPVAYPAVFIKVGSVVGETFIRNLKSFNIL 867

BLAST of MS016986 vs. ExPASy TrEMBL
Match: A0A6J1HSX1 (uncharacterized protein LOC111467157 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467157 PE=3 SV=1)

HSP 1 Score: 1637.9 bits (4240), Expect = 0.0e+00
Identity = 780/881 (88.54%), Postives = 826/881 (93.76%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           M NLKITKKHHIH NNPFPSAPTS P V+GDLSANFQALP  +VFSIGQDFQLLWR ENG
Sbjct: 13  MKNLKITKKHHIHLNNPFPSAPTSFPLVQGDLSANFQALPPYRVFSIGQDFQLLWRSENG 72

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSI+HLSQPTRSIWSTIPG+AFVSAAMVETEVEESRGSF+VKDGA+ LVCNHQT+DDI
Sbjct: 73  GSLSIHHLSQPTRSIWSTIPGRAFVSAAMVETEVEESRGSFSVKDGAIRLVCNHQTIDDI 132

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLKDAQFPMLLINGRIFNTKKKMTRRKKKNRL 180
           + ING D+ELEV+DHHFPSGYL LDQK H KDAQFPMLLINGRIFNTKKKM ++KK N L
Sbjct: 133 KEINGCDYELEVRDHHFPSGYLDLDQKSHKKDAQFPMLLINGRIFNTKKKMIKKKKNNSL 192

Query: 181 QETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDKF 240
           QETGFN         PPASARYWVLFEQKN+SQIGFQVMLGQPSYE     HSRGR ++ 
Sbjct: 193 QETGFN--------VPPASARYWVLFEQKNNSQIGFQVMLGQPSYE----YHSRGRLNRL 252

Query: 241 KFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKER 300
           KFRLHRL+K KVEW+WSLAKLK CVRVSSSEEE E L++ EEF+ FNRVC +Y+SEEKER
Sbjct: 253 KFRLHRLRKGKVEWHWSLAKLKRCVRVSSSEEEREDLKAPEEFDAFNRVCLSYSSEEKER 312

Query: 301 FFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYM 360
           FFGFGEQFSHMDFKGKRVPI VQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY+
Sbjct: 313 FFGFGEQFSHMDFKGKRVPILVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFYI 372

Query: 361 TSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPPE 420
           TSR+RSLYLEGYEYSVFDLTKN+ VQIQI+GNSIQG ILHGNSPSELIERFT+TIGRPPE
Sbjct: 373 TSRLRSLYLEGYEYSVFDLTKNNMVQIQIYGNSIQGRILHGNSPSELIERFTETIGRPPE 432

Query: 421 LPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWEV 480
           LPGWIISGAVVGMQGGTDAVRQIWD LK ++VPISAFWLQDWVGQRETVIGSQLWWNWEV
Sbjct: 433 LPGWIISGAVVGMQGGTDAVRQIWDVLKAHEVPISAFWLQDWVGQRETVIGSQLWWNWEV 492

Query: 481 DTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNGE 540
           DTTRYCGWKQLIKDL AQHIKVMTYCNPCLAP D+KQNRRRNLY+EAKELGIL+ KKNGE
Sbjct: 493 DTTRYCGWKQLIKDLDAQHIKVMTYCNPCLAPIDQKQNRRRNLYQEAKELGILV-KKNGE 552

Query: 541 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCED 600
           PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMV+DGVRGWMADFGEGLP+DATLYSG ED
Sbjct: 553 PYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVNDGVRGWMADFGEGLPIDATLYSG-ED 612

Query: 601 PIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEG 660
           PI AHNRYPE+WAQ+NREFADEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWEG
Sbjct: 613 PITAHNRYPEMWAQINREFADEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWEG 672

Query: 661 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 720
           DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL
Sbjct: 673 DQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMEL 732

Query: 721 NAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVC 780
           NAFTT+FRTHEGNKP+CNSQFYSNDRTL+QFARFAKVY AWKFYR+QLVKEAAQ+GLPVC
Sbjct: 733 NAFTTVFRTHEGNKPTCNSQFYSNDRTLSQFARFAKVYYAWKFYRIQLVKEAAQRGLPVC 792

Query: 781 RHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEL 840
           RHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE+
Sbjct: 793 RHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGEI 852

Query: 841 YTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           Y KPGCE+ VDAPV YPAVFIKVGS+VGETF+RNLK+YNIL
Sbjct: 853 YAKPGCEINVDAPVAYPAVFIKVGSVVGETFIRNLKSYNIL 879

BLAST of MS016986 vs. ExPASy TrEMBL
Match: A0A0A0L3I8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G430880 PE=3 SV=1)

HSP 1 Score: 1630.2 bits (4220), Expect = 0.0e+00
Identity = 774/882 (87.76%), Postives = 828/882 (93.88%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           MTNLK+TKKHHIH NNPFPS P S P ++G+LSAN+QAL + K FSIG+DFQLLWR +NG
Sbjct: 21  MTNLKVTKKHHIHLNNPFPSPPPSFPLLQGELSANYQALSSYKFFSIGKDFQLLWRSDNG 80

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSIYHLS PTRSIWSTI GQAFVSAAMVETEVEESRGSFAVKDGAVHL+CNHQT+DDI
Sbjct: 81  GSLSIYHLSDPTRSIWSTISGQAFVSAAMVETEVEESRGSFAVKDGAVHLICNHQTIDDI 140

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLK-DAQFPMLLINGRIFNTKKKMTRRKKKNR 180
           + ING DHE EVK+HHFPSGYLGLD K + K DAQFPMLLI+GRIFNT+KK    KKKN+
Sbjct: 141 KEINGCDHEFEVKEHHFPSGYLGLDLKNYEKEDAQFPMLLISGRIFNTEKK-RMMKKKNK 200

Query: 181 LQETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDK 240
           LQET FNGD+K N +   ASARYWV FEQK+SSQIGFQVMLGQPSYE RQ+AHSRG F++
Sbjct: 201 LQETSFNGDVKCNSKVLSASARYWVFFEQKSSSQIGFQVMLGQPSYEHRQIAHSRGGFNR 260

Query: 241 FKFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKE 300
            KFRLHRL+KRK EW+WSL KLKG VRV SSE+E+E LR+AEEFE FNRVC TY+SEEKE
Sbjct: 261 LKFRLHRLRKRKFEWHWSLTKLKGFVRVPSSEKEVEVLRAAEEFEAFNRVCLTYSSEEKE 320

Query: 301 RFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY 360
           RFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANL+SYRAGGDWSTTYAPSPFY
Sbjct: 321 RFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLISYRAGGDWSTTYAPSPFY 380

Query: 361 MTSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPP 420
           MTS+MRSLYLEGYEYS+FDLTKNDRVQIQIHGNS+QG ILHGNSPSELIERFT+TIGRPP
Sbjct: 381 MTSKMRSLYLEGYEYSIFDLTKNDRVQIQIHGNSVQGRILHGNSPSELIERFTETIGRPP 440

Query: 421 ELPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWE 480
           ELPGWIISGAVVGMQGGT+ VR+IWD+LK ++VPISAFWLQDWVGQRETVIGSQLWWNWE
Sbjct: 441 ELPGWIISGAVVGMQGGTNVVRKIWDELKAHEVPISAFWLQDWVGQRETVIGSQLWWNWE 500

Query: 481 VDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNG 540
           VD TRY GWKQLIKDLGA+HIKVMTYCNPCLAPTDEKQNRRRNLYEEAK LGILIKKKNG
Sbjct: 501 VDATRYSGWKQLIKDLGARHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKALGILIKKKNG 560

Query: 541 EPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCE 600
           EPYMVPNTAFDVGMLDLTHPNTSSWFK+ILQEMV+DGVRGWMADFGEGLPVDATLYSG E
Sbjct: 561 EPYMVPNTAFDVGMLDLTHPNTSSWFKKILQEMVNDGVRGWMADFGEGLPVDATLYSG-E 620

Query: 601 DPIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWE 660
           DPI AHNRYPEIWAQ+NREF DEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWE
Sbjct: 621 DPITAHNRYPEIWAQINREFVDEWKSKLVGKEKEDPEEALVFFMRAGFRNSPKWGMLFWE 680

Query: 661 GDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWME 720
           GDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWME
Sbjct: 681 GDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWME 740

Query: 721 LNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPV 780
           LNAFTT+FRTHEGNKPSCNSQFYS+DRTL+QFARFAKVYSAWKFYR+QLVKEAA++GLPV
Sbjct: 741 LNAFTTVFRTHEGNKPSCNSQFYSSDRTLSQFARFAKVYSAWKFYRIQLVKEAAERGLPV 800

Query: 781 CRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE 840
           CRHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKNNV AYFPLG++SSWQHIWTGE
Sbjct: 801 CRHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNNVNAYFPLGDNSSWQHIWTGE 860

Query: 841 LYTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           +Y K GCE+KVDAPVGYPAVFIKVGSIVGETF+RNLK +NIL
Sbjct: 861 VYAKLGCEIKVDAPVGYPAVFIKVGSIVGETFIRNLKMFNIL 900

BLAST of MS016986 vs. ExPASy TrEMBL
Match: A0A5A7SLB8 (Sulfoquinovosidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold34G002350 PE=3 SV=1)

HSP 1 Score: 1628.6 bits (4216), Expect = 0.0e+00
Identity = 778/882 (88.21%), Postives = 825/882 (93.54%), Query Frame = 0

Query: 1   MTNLKITKKHHIHFNNPFPSAPTSLPSVEGDLSANFQALPAIKVFSIGQDFQLLWRFENG 60
           MTNLKITKKHHIH NNPFPS PTS P ++G+LSANFQ L + K FSIG+DFQLLWR +NG
Sbjct: 1   MTNLKITKKHHIHLNNPFPSPPTSFPLLQGELSANFQVLSSYKFFSIGKDFQLLWRSDNG 60

Query: 61  GSLSIYHLSQPTRSIWSTIPGQAFVSAAMVETEVEESRGSFAVKDGAVHLVCNHQTVDDI 120
           GSLSIYHLS PTRSIWSTI GQAFVSAAMVETEVEESRGSFAVKDGAVHL+CNHQT+DDI
Sbjct: 61  GSLSIYHLSDPTRSIWSTISGQAFVSAAMVETEVEESRGSFAVKDGAVHLICNHQTIDDI 120

Query: 121 RVINGWDHELEVKDHHFPSGYLGLDQKMHLK-DAQFPMLLINGRIFNTKKKMTRRKKKNR 180
           + ING DHELEVK+HHFPSGYLGLD K + K DA+FPMLLI+GRIFNT++K    KKKN+
Sbjct: 121 KEINGCDHELEVKEHHFPSGYLGLDLKNYEKEDARFPMLLISGRIFNTERK-KMMKKKNK 180

Query: 181 LQETGFNGDLKYNPRAPPASARYWVLFEQKNSSQIGFQVMLGQPSYECRQMAHSRGRFDK 240
           LQET FNGD+K N +   ASARYW+LFEQK+SSQIGFQVMLGQPSYE RQ+AHSRG F++
Sbjct: 181 LQETSFNGDVKCNSKVLSASARYWLLFEQKSSSQIGFQVMLGQPSYEYRQIAHSRGGFNR 240

Query: 241 FKFRLHRLKKRKVEWYWSLAKLKGCVRVSSSEEEMEGLRSAEEFEGFNRVCFTYTSEEKE 300
            KFR HRL+KRK EW WSL KLKG VRV SSEEEME LR+AEEFE FNR C TY+SEEKE
Sbjct: 241 LKFRWHRLRKRKFEWRWSLTKLKGFVRVCSSEEEMEVLRAAEEFEAFNRACLTYSSEEKE 300

Query: 301 RFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY 360
           RFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY
Sbjct: 301 RFFGFGEQFSHMDFKGKRVPIFVQEQGIGRGDQPITFAANLVSYRAGGDWSTTYAPSPFY 360

Query: 361 MTSRMRSLYLEGYEYSVFDLTKNDRVQIQIHGNSIQGWILHGNSPSELIERFTDTIGRPP 420
           MTS+MRSLYLEGYEYS+FDLTKNDRVQIQIHGNSIQG ILHGNSPSELIE FT+TIGRPP
Sbjct: 361 MTSKMRSLYLEGYEYSIFDLTKNDRVQIQIHGNSIQGRILHGNSPSELIECFTETIGRPP 420

Query: 421 ELPGWIISGAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQDWVGQRETVIGSQLWWNWE 480
           ELPGWIISGAVVGMQGGT+ VR+IWD+LK ++VPISAFWLQDWVGQRETVIGSQLWWNWE
Sbjct: 421 ELPGWIISGAVVGMQGGTNIVRKIWDELKAHEVPISAFWLQDWVGQRETVIGSQLWWNWE 480

Query: 481 VDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKELGILIKKKNG 540
           VD TRY GWKQLIKDLGA+HIKVMTYCNPCLAPTDEKQNRRRNLYEEAK LGIL+KKKNG
Sbjct: 481 VDATRYSGWKQLIKDLGARHIKVMTYCNPCLAPTDEKQNRRRNLYEEAKALGILVKKKNG 540

Query: 541 EPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSGCE 600
           EPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSG E
Sbjct: 541 EPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVDDGVRGWMADFGEGLPVDATLYSG-E 600

Query: 601 DPIAAHNRYPEIWAQMNREFADEWKSKLVGKEKEDPHEALVFFMRAGFRNSPKWGMLFWE 660
           DPI AHNRYPEIWAQ+NREF DEWKSKLVGKEKEDP EALVFFMRAGFRNSPKWGMLFWE
Sbjct: 601 DPITAHNRYPEIWAQINREFVDEWKSKLVGKEKEDPQEALVFFMRAGFRNSPKWGMLFWE 660

Query: 661 GDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWME 720
           GDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWME
Sbjct: 661 GDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWME 720

Query: 721 LNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPV 780
           LNAFTT+FRTHEGNKPSCNSQFYSN+RTL+QFARFAKVYSAWKFYR+QLVKEAAQ+GLPV
Sbjct: 721 LNAFTTVFRTHEGNKPSCNSQFYSNNRTLSQFARFAKVYSAWKFYRIQLVKEAAQRGLPV 780

Query: 781 CRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGE 840
           CRHLFVHYPEDEYVLTL HQQFLVGSEILVVPVLDKGKN VKAYFPL +SSSWQHIWTGE
Sbjct: 781 CRHLFVHYPEDEYVLTLGHQQFLVGSEILVVPVLDKGKNYVKAYFPLDDSSSWQHIWTGE 840

Query: 841 LYTKPGCEVKVDAPVGYPAVFIKVGSIVGETFVRNLKNYNIL 882
           +Y K GCE+KVDAPVGYPAVFIKVGSIVGETF+RNLK +NIL
Sbjct: 841 VYAKLGCEIKVDAPVGYPAVFIKVGSIVGETFIRNLKMFNIL 880

BLAST of MS016986 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.8e-24
Identity = 127/533 (23.83%), Postives = 215/533 (40.34%), Query Frame = 0

Query: 353 YAPSPFYMTSRMRSLYLEGYEYSVFDLT--------KNDRVQIQIHGNSIQGWILHGNSP 412
           Y   P YM   +R++  + Y +SV  L         + D +  ++ G     +   G SP
Sbjct: 211 YGSHPVYMD--LRNVSGKAYAHSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSP 270

Query: 413 SELIERFTDTIGRPPELPGWIISGAVVGM-QGGTDAVRQIWDDLKVYKVPISAFWLQDWV 472
             +++++T  IGRP  +P W +              V+ + D+ +  K+P+   W     
Sbjct: 271 LNVVDQYTSLIGRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIW----- 330

Query: 473 GQRETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNL 532
              + + G   + ++ +D   +   K L+  L   H   M Y    +       N    +
Sbjct: 331 NDADYMDG---YKDFTLDLVNFPHAK-LLSFLDRIHKMGMKYV--VIKDPGIGVNASYGV 390

Query: 533 YEEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSW-------FKEILQ------ 592
           Y+      + IK + G+P++       V   D  +P T SW       F E++       
Sbjct: 391 YQRGMASDVFIKYE-GKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIRRFHELVPIDGLWI 450

Query: 593 EMVDDGVRGWMADFG-EGLPVDATLYSGCEDPIAAHNRY--PEIWAQMNREFADEWKSKL 652
           +M +    G  A  G + +P  A  Y+G  +   AH+ Y   E  A      A + K   
Sbjct: 451 DMNEINATGHKASLGFKTIPTSAYHYNGVRE-YDAHSIYGFSEAIATHKALLAVQGKRPF 510

Query: 653 VGKEKEDPHEALVFFMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGY 712
           +               R+ F  S ++    W GD   +WQ+   ++ +++ +L+ G+ G 
Sbjct: 511 I-------------LSRSTFVGSGQYA-AHWTGDNQGTWQS---LQVSISTMLNFGIFGV 570

Query: 713 AFNHSDIGGYCAVNLPFIKYRRSEELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRT 772
               SDI G+             EEL  RW+E+ AF    R H        + +Y+  + 
Sbjct: 571 PMVGSDICGFFPPT--------PEELCNRWIEVGAFYPFSRDH--------ADYYAPRKE 630

Query: 773 LTQFARFAKVYSAWKFYRVQLVK-------EAAQKGLPVCRHLFVHYPEDEYVLTLSHQQ 832
           L Q+   A+        R +L+        EA   G P+ R LF  +PE      LS +Q
Sbjct: 631 LYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMSGAPIARPLFFSFPEFTECYGLS-KQ 690

Query: 833 FLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIW---------TGELYTKP 845
           FL+GS +++ PVL++GK  V+A FP G   SW H++          G L+T P
Sbjct: 691 FLLGSSLMISPVLEQGKTQVEALFPPG---SWYHMFDMTQVVVSKNGRLFTLP 691

BLAST of MS016986 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 108.2 bits (269), Expect = 3.4e-23
Identity = 129/553 (23.33%), Postives = 217/553 (39.24%), Query Frame = 0

Query: 353 YAPSPFYMTSR-MRSLYLEGYEYSVFDLTKN--------DRVQIQIHGNSIQGWILHGNS 412
           Y   PFYM  R  +     G  + V  L  N         R+   + G  I  ++  G S
Sbjct: 230 YGSHPFYMDVRGSKGNEEAGTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPS 289

Query: 413 PSELIERFTDTIGRPPELPGWIIS--GAVVGMQGGTDAVRQIWDDLKVYKVPISAFWLQ- 472
           P  ++ ++T+ IGRP  +P W         G +  +D +  + D      +P+   W   
Sbjct: 290 PEMVMNQYTELIGRPAPMPYWSFGFHQCRYGYKNVSD-LEYVVDGYAKAGIPLEVMWTDI 349

Query: 473 DWV-GQRETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNR 532
           D++ G ++  +      N+  D       +  +  L     K +   +P +       + 
Sbjct: 350 DYMDGYKDFTLDPV---NFPEDK-----MQSFVDTLHKNGQKYVLILDPGIG-----VDS 409

Query: 533 RRNLYEEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSS-WFKEI--LQEMVD-D 592
               Y    E  + I K+NGEPY+       V   D  +P  ++ W  EI   QE++  D
Sbjct: 410 SYGTYNRGMEADVFI-KRNGEPYLGEVWPGKVYFPDFLNPAAATFWSNEIKMFQEILPLD 469

Query: 593 GVRGWMADFGEGLPVDATLYSGCEDPIAAHNRYPEIWAQMNR----------EFADEWKS 652
           G+   M +    +    +  S  +DP    N   +     N+            ++    
Sbjct: 470 GLWIDMNELSNFITSPLSSGSSLDDPPYKINNSGDKRPINNKTVPATSIHFGNISEYDAH 529

Query: 653 KLVG-KEKEDPHEALV--------FFMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAV 712
            L G  E +  H+A+V           R+ F +S K+    W GD    W   + +  ++
Sbjct: 530 NLYGLLEAKATHQAVVDITGKRPFILSRSTFVSSGKY-TAHWTGDNAAKW---EDLAYSI 589

Query: 713 TGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEELLLRWMELNAFTTIFRTHEGNKPS 772
            G+L+ GL G     +DI G+         +  +EEL  RW++L AF    R H  +  +
Sbjct: 590 PGILNFGLFGIPMVGADICGF--------SHDTTEELCRRWIQLGAFYPFARDH-SSLGT 649

Query: 773 CNSQFYSNDRTLTQFARFAKVYSAWKFYRVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTL 832
              + Y  D   +   +   +      +   L+ EA   G P+ R LF  +P+D     +
Sbjct: 650 ARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGNPIARPLFFSFPQDTKTYEI 709

Query: 833 SHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSSWQHIWTGELYT--KPGCEVKVDAPV 868
              QFL+G  I+V P L +G   V AYFP G   +W  ++          G  V++D P 
Sbjct: 710 D-SQFLIGKSIMVSPALKQGAVAVDAYFPAG---NWFDLFNYSFAVGGDSGKHVRLDTPA 750

BLAST of MS016986 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 107.5 bits (267), Expect = 5.7e-23
Identity = 132/578 (22.84%), Postives = 228/578 (39.45%), Query Frame = 0

Query: 353 YAPSPFYMTSRMRSLYLEGYEYSVFDLTKN--------DRVQIQIHGNSIQGWILHGNSP 412
           Y   P YM   +R++  + Y ++V  L  N        D +  ++ G     + + G SP
Sbjct: 214 YGSHPMYMD--LRNVGGKAYAHAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSP 273

Query: 413 SELIERFTDTIGRPPELPGWIISGAVVGM-QGGTDAVRQIWDDLKVYKVPISAFWLQD-- 472
             +++++T  IGRP  +P W +              V  + D+ K  K+P+   W  D  
Sbjct: 274 LNVVDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDH 333

Query: 473 WVGQRETVIGSQLWWNWEVDTTRYCGWKQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRR 532
             G ++  +    +        +   +   I  +G ++I +    +P +       N   
Sbjct: 334 MDGHKDFTLNPVAY-----PRAKLLAFLDKIHKIGMKYIVIN---DPGIG-----VNASY 393

Query: 533 NLYEEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSWFKEILQEMVD----DGV 592
             ++ A    + IK + G+P++       V   D  +P T SW+ + ++   D    DG+
Sbjct: 394 GTFQRAMAADVFIKYE-GKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGL 453

Query: 593 ------------------RGWMADFGEG------LPVDATLYSGCEDPIAAHNRYPEIWA 652
                              G     GEG      L       +  +DP    N    + A
Sbjct: 454 WIDMNEVSNFCSGLCTIPEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINA-TGVVA 513

Query: 653 QMNREFADEWKSKLVGKEKEDPHEALVF--------------------FMRAGFRNSPKW 712
            +  +      +   G  + D H    F                      R+ F  S ++
Sbjct: 514 PVGFKTIATSATHYNGVREYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQY 573

Query: 713 GMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAFNHSDIGGYCAVNLPFIKYRRSEEL 772
               W GD   +WQ+   ++ +++ +L+ G+ G     SDI G+          + +EEL
Sbjct: 574 A-AHWTGDNQGTWQS---LQVSISTMLNFGIFGVPMVGSDICGFYP--------QPTEEL 633

Query: 773 LLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRTLTQFARFAKVYSAWKF--YRVQLVKE 832
             RW+E+ AF    R H  N  S   + Y  D T+   AR A +   +K   +   L  E
Sbjct: 634 CNRWIEVGAFYPFSRDH-ANYYSPRQELYQWD-TVADSARNA-LGMRYKILPFLYTLNYE 693

Query: 833 AAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVGSEILVVPVLDKGKNNVKAYFPLGESSS 868
           A   G P+ R LF  +PE       S +QFL+GS  ++ PVL++GK  V+A FP G   S
Sbjct: 694 AHMTGAPIARPLFFSFPEYTECYGNS-RQFLLGSSFMISPVLEQGKTEVEALFPPG---S 753

BLAST of MS016986 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 99.4 bits (246), Expect = 1.6e-20
Identity = 106/420 (25.24%), Postives = 182/420 (43.33%), Query Frame = 0

Query: 474 LWWNWE-VDTTRYCGW--------KQLIKDLGAQHIKVMTYCNPCLAPTDEKQNRRRNLY 533
           LW + E  D  RY  W        +++ K L A+  K++T  +P +     K++    L+
Sbjct: 396 LWLDIEHTDGKRYFTWDSVLFPHPEEMQKKLAAKGRKMVTIVDPHI-----KRDDSYFLH 455

Query: 534 EEAKELGILIKKKNGEPYMVPNTAFDVGMLDLTHPNTSSW------FKEILQEMVDDGVR 593
           +EA ++G  +K  +G+ +           +D+  P    W      +K  +       + 
Sbjct: 456 KEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGS--TPSLY 515

Query: 594 GWMADFGE---------GLPVDATLYSGCEDPIAAHNRYPEIWAQMNREFADEWKSKLVG 653
            W  D  E          +P DA L+ G  +    HN Y   +   +   +D    +  G
Sbjct: 516 TW-NDMNEPSVFNGPEVTMPRDA-LHVGGVEHREVHNAYGYYF---HMATSDGLVMREEG 575

Query: 654 KEKEDPHEALVFFMRAGFRNSPKWGMLFWEGDQMVSWQANDGIKSAVTGLLSSGLSGYAF 713
           K++           RA F  + ++G + W GD    W   + ++ ++  +L+ GL+G  F
Sbjct: 576 KDRP------FVLSRAIFPGTQRYGAI-WTGDNTAEW---EHLRVSIPMILTLGLTGITF 635

Query: 714 NHSDIGGYCAVNLPFIKYRRSEELLLRWMELNAFTTIFRTHEGNKPSCNSQFYSNDRTLT 773
           + +DIGG+     P        ELL+RW ++ A+   FR H  +       +   +R  T
Sbjct: 636 SGADIGGFFGNPEP--------ELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERN-T 695

Query: 774 QFARFAKVYSAWKF--YRVQLVKEAAQKGLPVCRHLFVHYPEDEYVLTLSHQQFLVGSEI 833
           +  R A +++ +    Y   L +EA   G+PV R L++ +P+DE   + + + F+VGS +
Sbjct: 696 ELMRDA-IHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFS-NDEAFMVGSGL 755

Query: 834 LVVPVLDKGKNNVKAYFPLGESSSWQHIWTGELYTKPGCEVKVDAPVGYPAVFIKVGSIV 868
           LV  V  KG      Y P  E  SW  +  G+ Y   G   K+DAP      F K G+I+
Sbjct: 756 LVQGVYTKGTTQASVYLPGKE--SWYDLRNGKTYV-GGKTHKMDAPEESIPAFQKAGTII 779

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153908.10.0e+0099.32uncharacterized protein LOC111021314 isoform X1 [Momordica charantia][more]
XP_038882634.10.0e+0090.92sulfoquinovosidase-like [Benincasa hispida][more]
KAG6603308.10.0e+0089.22hypothetical protein SDJN03_03917, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022932849.10.0e+0088.99uncharacterized protein LOC111439390 isoform X1 [Cucurbita moschata][more]
XP_022967711.10.0e+0088.54uncharacterized protein LOC111467157 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P321383.0e-10936.49Sulfoquinovosidase OS=Escherichia coli (strain K12) OX=83333 GN=yihQ PE=1 SV=3[more]
Q9F2342.1e-3824.05Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1[more]
P967931.4e-3424.19Alpha-xylosidase XylQ OS=Lactiplantibacillus pentosus OX=1589 GN=xylQ PE=1 SV=1[more]
B3PEE61.6e-3322.18Oligosaccharide 4-alpha-D-glucosyltransferase OS=Cellvibrio japonicus (strain Ue... [more]
D2PPM77.3e-3123.981,3-alpha-isomaltosidase OS=Kribbella flavida (strain DSM 17836 / JCM 10339 / NB... [more]
Match NameE-valueIdentityDescription
A0A6J1DIT60.0e+0099.32uncharacterized protein LOC111021314 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1EY610.0e+0088.99uncharacterized protein LOC111439390 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1HSX10.0e+0088.54uncharacterized protein LOC111467157 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0L3I80.0e+0087.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G430880 PE=3 SV=1[more]
A0A5A7SLB80.0e+0088.21Sulfoquinovosidase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT3G45940.11.8e-2423.83Glycosyl hydrolases family 31 protein [more]
AT5G11720.13.4e-2323.33Glycosyl hydrolases family 31 protein [more]
AT1G68560.15.7e-2322.84alpha-xylosidase 1 [more]
AT5G63840.11.6e-2025.24Glycosyl hydrolases family 31 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.40.1760glycosyl hydrolase (family 31)coord: 239..401
e-value: 3.1E-20
score: 74.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 403..809
e-value: 4.0E-83
score: 281.5
NoneNo IPR availablePANTHERPTHR46959SULFOQUINOVOSIDASEcoord: 39..880
NoneNo IPR availableCDDcd14752GH31_Ncoord: 286..416
e-value: 3.1445E-22
score: 90.708
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 778..862
IPR000322Glycoside hydrolase family 31PFAMPF01055Glyco_hydro_31coord: 398..867
e-value: 1.6E-65
score: 222.1
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 810..862
e-value: 4.1E-5
score: 25.5
IPR044112Sulfoquinovosidase YihQ-likeCDDcd06594GH31_glucosidase_YihQcoord: 416..755
e-value: 0.0
score: 525.611
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 406..776
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 287..404

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016986.1MS016986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds