MS016969 (gene) Bitter gourd (TR) v1

Overview
NameMS016969
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontubulin-folding cofactor B
Locationscaffold197: 64981 .. 68376 (-)
RNA-Seq ExpressionMS016969
SyntenyMS016969
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCCGATTGCAGGAGATCGAAGCTGATGAATCTGTACTCTTGCGCGTAACACATGCCAATCTCAAGTCCTTCACTTCTGATGTTCGTTTTTCCCTTCAGGTATTTCCATTTTCCCAAAGATCGAACGTTATTTTAGTGTTCGTCGCATCAATTGCCGATTGCCCATTATGGGTTTTGTTTAAAAATTGGTTTTGGGTGTCTTAAATTGTAGATGAGCATAGAATCTGTCAAGGAAAAGCTGTGGAAAAAATGTGGTACTTCTGTAAACTCCATGTGCCTTGAACTCTATGATGACTCCGGTGCTAAAATTTCTGATTTGACGGATAATTCCTTGCCCTTGGGATTTTATTCCCCCCTAGATGGGTACTTCTTCTCCCATTTTCTTTCAATGGGTTCTTTACTAATTTTTACACTGCATTTGCTTTGTTTTCCACTCTGCAAATGGTAATTTATATCCCACTGTTGATGAGAAATTTTAATATGTGGATTGTTTCTTTGAAAAGGTACCGGCTACATATTATTGATCTTGACCCCTCATCAGTTACTTCTGGTGGTTGGCTTGAAGATACCTCACTAGTGGAGAAGTACCAGATTTCTGAAGAAGCTTACGATAAACGTGATGGTTAGTTTCTTGCATAGCTTCCCCTGCTATTAGAAAGCTTGTATGTAATTGCCTCGATTGCTTGTCGAATAGGAAGCTCTCTCTGCACTGAAGGACCCCTCCGCCACCAATGGGGTATTGTGAGTCAGAGCCAAGAATTATAGTATAAACAGGCTTAGATTTTGGTCTTAACAATATGAGGTTGATAGCTATTTTCCAAAATTTTATTGAATCTGCATTTTCTTACGACATGAAGCAGCTGCTATTTAGAAAGCTTGTGGAATGTTAAATGTGTGTGCTTAATTTTTCAAAGAATGTGGGAGAACTTCTTTGAGCCAGTAAGCAGCGATTTTGTGGGAAGTGTGGCTCAAATGAGATAGGAGATTTTTCTCTGGGTAAGAGAGGATTACGGATTAGCTGTAGGAAGTAATTGTTTTTCATCCTTCTTAGTGTCTTAGATCTAAATACTTTTGTAAATATTTTCTTTCCTCTATACAAACCAATTGAGATCTTCAGATTGTTTTTCTGCAATAGCTACCTCTTGTATATTTCAACGAAATAGTTCATCATGAAAAGGTATTGTGCATAATTTACCTGAACTAGTTCATCAGGAAAAGGTATTGTGCATAATTTACCTGAAATAGTTCATCATGAAAAGGTATTGTGCATAATTTACCTACTTTTGGATTTTTTTTCAGATACATTCCGAAAATTCAAGGAAAAATTGGCATCCCAGAACCCATCAGCTTTTGAAAGTAAAGTGAGCCTTCTGTATCCTACTTTTGTATTTAACAATTTCCATAGAAACCTTGTTCATCACTAGTGAATTGTAAATTTGATGTAGAGGCTACCAGTACTTTAGTTAAGTGGAGCTGTGTAAGTGCCATCTCTTAGGATCCTTGAAACCTGGTTTTCATCATGCATTATTGTGAAAATCAGATGCATTTCCATCTCCGTACTTGTCATTTTTGGTCTCTATTTACTGACCAGCCATAAATTTACAGATATCTGATACCTACATGGAAGAACTCTGTGCAAATATCAAGGTTAGTTACCTGCTTTAGATGTGTATAACGTATGTATTCTTTAAAACCTACGAGAATCCTTATTTTGAACAGTTAGAACTTTCATGCTTAAAGTTTTTTGGACGAGACTTCATGTTAACCTATGATTTCCTGGAGACAGTTCGGAAAGATTTTAGAATAGTGAAATATTTGTTTCTTGGTCTTCGCACAGTAATTTTTCAGTCATACATATCTATGTTCTGCTCTGATAACTTTTGTTCTGTCAAGTCATCTCTAAGTTTCCTTGAACAAATCCATGGTTTAAGCAGTCCTTTAAATTTTGGCCTGATAGTTCTAATAAATGCCCCTCTTCTCTTCTTTGTTCGTCCTGAATCGGAACATTTTACTCAATGTCGACTTCAGATAGTTAGCATAAATCTTGCGTCCAGAAATTCTTTCATGGGGGAAGTACTCAACTGCTCCTTCAGAGTTCAGACCTCCCTTTTAGTCGCAGTTTGTGCTAAACTTTGACCTTTTTCATAATTTAGGTAGGTGATAGATGTGAAATTGAACCAGGGGAGAAGAGGGGTGTTGTAAAATTTGTTGGCCGGGCTGAATCGCTTGCTCCTGGATTTTGGGTTGGAGTTCAATATGATGAACCATTGGGGAAAAACGATGGCATGTAAGAAAGCCTTTCACTCTCTTCCGCCTGTGATTAATTTGTTTTTCCTTATATTTTTTTTTCGTTATTCCTTTTGAGTAACTCTAGTACTTCGATAATCACAAATGCACACAAAAAAGTTTCTCTTTGAGAGGGACAAAATATACTACGGACTTTGAAACATATATTTTATGATGACAACCTGCCTCAATTTGGGTAAGTTGTTTCATGTGGCTGTTCTGTTACAGAACAATTTTCACTCTGATCATTCTTAAATTCTTTAGGTTTCAACCTTGTCTACTACTTCTTGGGTCATTGTTTGATAAAGTTGTCATCTAAGAAGCACAGACCCGGGACACATCCACGGTGACACACCAATTTCTAAAAATGTAGGACATGAACATGTCCGGGTCATGTTATTTTTTAAAAAAATCTTTTAGATAATATATGTGTATATATTGATGTAGTAATACTTTTAAATGAATTAACCATACAAATTGAATATAACAAAACAAAATGTGCTTCGTTAATACATAAATGACGTAGTAGAGGGAGGGCAGTCAAAAGTGGAGGCGAAGGCAAAGAGCAATTGGAGGGAGGGAGCATTTTTACGAGAATAGTTCTCCTTTGTTTTTTTCCTTTGTTTTTTTCCTTCTCTAAATTATTTCTTTTTTAGAGGATTTTTAGTGCTTGATGGGCTGGCTGGCTGACCTTTTGTTTTTCATTTGGTTTCTTTTAAGCGGGCTTATATTTTTCTTTAAATTTCATGTAAATTTCATATGGTTTGTCCTTGGTGTGTCCACCACATGTCCAAAATATTTTTAAAAAAGAAAGAAAAAGACACATAGTTTCGTGTGTCGGACATGTGTCCATGCTTCCTAAGTTATCCTCATTCATTCGGGTTGACAGATCAGAATTGCGTTAGAATCCAGCTTTTTGTCGATTCGATAAAGTGGTTCTTCCCAGTTTATTGTTTATGCTGCACTCTTCAGAGTTTTTTATCACATGTTGTGTATACTGAACTAGTGAAACTCGGCAGGGTAAAAGGGATCCGCTATTTTGATTGTCCTCCTCTTCATGGTGCGATGGTTAGGCCAGACAAAGTGAAGGTTTGTTTTGCAGCT

mRNA sequence

ATGGCGTCCCGATTGCAGGAGATCGAAGCTGATGAATCTGTACTCTTGCGCGTAACACATGCCAATCTCAAGTCCTTCACTTCTGATGTTCGTTTTTCCCTTCAGATGAGCATAGAATCTGTCAAGGAAAAGCTGTGGAAAAAATGTGGTACTTCTGTAAACTCCATGTGCCTTGAACTCTATGATGACTCCGGTGCTAAAATTTCTGATTTGACGGATAATTCCTTGCCCTTGGGATTTTATTCCCCCCTAGATGGGTACCGGCTACATATTATTGATCTTGACCCCTCATCAGTTACTTCTGGTGGTTGGCTTGAAGATACCTCACTAGTGGAGAAGTACCAGATTTCTGAAGAAGCTTACGATAAACGTGATGATACATTCCGAAAATTCAAGGAAAAATTGGCATCCCAGAACCCATCAGCTTTTGAAAGTAAAATATCTGATACCTACATGGAAGAACTCTGTGCAAATATCAAGGTAGGTGATAGATGTGAAATTGAACCAGGGGAGAAGAGGGGTGTTGTAAAATTTGTTGGCCGGGCTGAATCGCTTGCTCCTGGATTTTGGGTTGGAGTTCAATATGATGAACCATTGGGGAAAAACGATGGCATGGTAAAAGGGATCCGCTATTTTGATTGTCCTCCTCTTCATGGTGCGATGGTTAGGCCAGACAAAGTGAAGGTTTGTTTTGCAGCT

Coding sequence (CDS)

ATGGCGTCCCGATTGCAGGAGATCGAAGCTGATGAATCTGTACTCTTGCGCGTAACACATGCCAATCTCAAGTCCTTCACTTCTGATGTTCGTTTTTCCCTTCAGATGAGCATAGAATCTGTCAAGGAAAAGCTGTGGAAAAAATGTGGTACTTCTGTAAACTCCATGTGCCTTGAACTCTATGATGACTCCGGTGCTAAAATTTCTGATTTGACGGATAATTCCTTGCCCTTGGGATTTTATTCCCCCCTAGATGGGTACCGGCTACATATTATTGATCTTGACCCCTCATCAGTTACTTCTGGTGGTTGGCTTGAAGATACCTCACTAGTGGAGAAGTACCAGATTTCTGAAGAAGCTTACGATAAACGTGATGATACATTCCGAAAATTCAAGGAAAAATTGGCATCCCAGAACCCATCAGCTTTTGAAAGTAAAATATCTGATACCTACATGGAAGAACTCTGTGCAAATATCAAGGTAGGTGATAGATGTGAAATTGAACCAGGGGAGAAGAGGGGTGTTGTAAAATTTGTTGGCCGGGCTGAATCGCTTGCTCCTGGATTTTGGGTTGGAGTTCAATATGATGAACCATTGGGGAAAAACGATGGCATGGTAAAAGGGATCCGCTATTTTGATTGTCCTCCTCTTCATGGTGCGATGGTTAGGCCAGACAAAGTGAAGGTTTGTTTTGCAGCT

Protein sequence

MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLELYDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVGRAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKVCFAA
Homology
BLAST of MS016969 vs. NCBI nr
Match: XP_022153874.1 (tubulin-folding cofactor B [Momordica charantia])

HSP 1 Score: 465.7 bits (1197), Expect = 2.4e-127
Identity = 228/229 (99.56%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL
Sbjct: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA
Sbjct: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCE+EPGEKRGVVKFVG
Sbjct: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEVEPGEKRGVVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 229

BLAST of MS016969 vs. NCBI nr
Match: XP_008455690.1 (PREDICTED: tubulin-folding cofactor B isoform X1 [Cucumis melo])

HSP 1 Score: 450.3 bits (1157), Expect = 1.0e-122
Identity = 218/233 (93.56%), Postives = 227/233 (97.42%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLW+KCGTSVNSMCLEL
Sbjct: 1   MASRLQQIEKDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWRKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSG+KISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEA
Sbjct: 61  YDDSGSKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           YDKRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRC++EPGEKRGVVKFVG
Sbjct: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKVCFAA 234
           RAESLAPGFWVGVQYDEPLGKNDG VKGI YFDC PLHGAMVRPDKVKV F+A
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGTVKGIHYFDCSPLHGAMVRPDKVKVQFSA 233

BLAST of MS016969 vs. NCBI nr
Match: KAG6595702.1 (Tubulin-folding cofactor B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 449.9 bits (1156), Expect = 1.3e-122
Identity = 218/229 (95.20%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLWKKCGTSVNSMCLEL
Sbjct: 1   MASRLQQIETDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWKKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSGAKISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA
Sbjct: 61  YDDSGAKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           Y+KRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRCE+EPG+ RG VKFVG
Sbjct: 121 YEKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCEVEPGKMRGSVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESLAPGFWVGVQYDEPLGKNDG VKGIRYFDCPPLHGAMVRPDKVKV
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGKVKGIRYFDCPPLHGAMVRPDKVKV 229

BLAST of MS016969 vs. NCBI nr
Match: XP_023517149.1 (tubulin-folding cofactor B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 449.1 bits (1154), Expect = 2.3e-122
Identity = 218/229 (95.20%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLWKKCGTSVNSMCLEL
Sbjct: 1   MASRLQQIETDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWKKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSGAKISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA
Sbjct: 61  YDDSGAKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           Y+KRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKV DRCE+EPG+ RG VKFVG
Sbjct: 121 YEKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVADRCEVEPGKIRGSVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 229

BLAST of MS016969 vs. NCBI nr
Match: KAG7027664.1 (Tubulin-folding cofactor B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 448.4 bits (1152), Expect = 3.9e-122
Identity = 217/228 (95.18%), Postives = 223/228 (97.81%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLWKKCGTSVNSMCLEL
Sbjct: 1   MASRLQQIETDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWKKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSGAKISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA
Sbjct: 61  YDDSGAKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           Y+KRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRCE+EPG+ RG VKFVG
Sbjct: 121 YEKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCEVEPGKMRGSVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVK 229
           RAESLAPGFWVGVQYDEPLGKNDG VKGIRYFDCPPLHGAMVRPDKVK
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGKVKGIRYFDCPPLHGAMVRPDKVK 228

BLAST of MS016969 vs. ExPASy Swiss-Prot
Match: Q67Z52 (Tubulin-folding cofactor B OS=Arabidopsis thaliana OX=3702 GN=TFCB PE=1 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 2.5e-100
Identity = 171/229 (74.67%), Postives = 201/229 (87.77%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MA+   ++E D+SV L +THANLKSF++D RFS QMS+E+VKEKLWKKCGTSVNSM LEL
Sbjct: 1   MATSRLQLEGDDSVHLHITHANLKSFSADARFSPQMSVEAVKEKLWKKCGTSVNSMALEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSG+K++ L+D+S PLGF+SP DG+RLHIIDLDPSSVT+GGWLEDTSLVEKY ISEE 
Sbjct: 61  YDDSGSKVAVLSDDSRPLGFFSPFDGFRLHIIDLDPSSVTTGGWLEDTSLVEKYNISEED 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           Y KR D+FRKFKEK  SQNP A E+K  + YME+LCANIKVGDRC++EPGEKRG+VK+VG
Sbjct: 121 YAKRTDSFRKFKEKRVSQNPVAAEAKTKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESL PG+WVG+QYDEPLGK+DGMVKG R+F+CP L G MVRPDKVKV
Sbjct: 181 RAESLGPGYWVGIQYDEPLGKHDGMVKGTRFFECPRLQGGMVRPDKVKV 229

BLAST of MS016969 vs. ExPASy Swiss-Prot
Match: Q20728 (Tubulin-specific chaperone B OS=Caenorhabditis elegans OX=6239 GN=tbcb-1 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-37
Identity = 90/213 (42.25%), Postives = 124/213 (58.22%), Query Frame = 0

Query: 22  NLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLELYDDSGAKISDLTDNSLPLGFY 81
           N   F  + ++   MS+  +K+KL    GT+V+SM ++L+D       +LTD +  L   
Sbjct: 12  NATDFPMEKKYPAGMSLNDLKKKLELVVGTTVDSMRIQLFDGDDQLKGELTDGAKSLKDL 71

Query: 82  SPLDGYRLHIIDLDPSSVTSGGW-LEDTSLVEKYQISEEAYDKRDDTFRKFKEKLASQNP 141
              DGYR+H +D     VT G    +D S+VEKY++S++ Y KR D+ R +K+K+  +  
Sbjct: 72  GVRDGYRIHAVD-----VTGGNEDFKDESMVEKYEMSDDTYGKRTDSVRAWKKKMQEEQG 131

Query: 142 SAFE-SKISDTYMEELCANIKVGDRCEIEPG---EKRGVVKFVGRAESLAPGFWVGVQYD 201
           SA      SD   EE   NI VG+RCE+  G    +RG V +VG A     G WVGV+YD
Sbjct: 132 SAAPMENESDKLNEEAAKNIMVGNRCEVTVGAQMARRGEVAYVG-ATKFKEGVWVGVKYD 191

Query: 202 EPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           EP+GKNDG V G+RYFDC P +G  VRP  VKV
Sbjct: 192 EPVGKNDGSVAGVRYFDCDPKYGGFVRPVDVKV 218

BLAST of MS016969 vs. ExPASy Swiss-Prot
Match: Q9D1E6 (Tubulin-folding cofactor B OS=Mus musculus OX=10090 GN=Tbcb PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 6.0e-33
Identity = 89/221 (40.27%), Postives = 122/221 (55.20%), Query Frame = 0

Query: 21  ANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLELYDDSGAKISDLTDNSLPLGF 80
           ++L SF S+ R+S  ++I   K KL    G+  + M LELY       S L      LG 
Sbjct: 17  SSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQEDALLGS 76

Query: 81  YSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYDKRDDTFRKF--------- 140
           Y   DG R+H+I  D S V  G + ED S VEKY+IS EAY++R +T R F         
Sbjct: 77  YPVDDGCRIHVI--DHSGVRLGEY-EDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPY 136

Query: 141 KEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGE---KRGVVKFVGRAESLAPG 200
            E+L +Q  +    ++S+   +   + I VG RCE+   +   +RG V +VG  +   PG
Sbjct: 137 NEELRAQQEAEAAQRLSEEKAQ--ASAISVGSRCEVRAPDHSLRRGTVMYVGLTD-FKPG 196

Query: 201 FWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           +WVGV+YDEPLGKNDG V G RYF+C   +GA V+P  V V
Sbjct: 197 YWVGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSAVTV 231

BLAST of MS016969 vs. ExPASy Swiss-Prot
Match: Q5E951 (Tubulin-folding cofactor B OS=Bos taurus OX=9913 GN=TBCB PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.8e-33
Identity = 88/219 (40.18%), Postives = 124/219 (56.62%), Query Frame = 0

Query: 21  ANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLELYDDSGAKISDLTDNSLPLGF 80
           ++L SF S  R+S  +++   K KL    G+  + M LELY         L  +   LG 
Sbjct: 17  SSLNSFRSQKRYSRSLTVAEFKCKLQLVVGSPASCMELELYGPDDKFCCKLDQDDALLGS 76

Query: 81  YSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYDKRDDTFRKF--KEKLASQ 140
           Y   DG R+H+ID   + +   G  ED S VEKY+IS+EAY++R D+ R F  + KL   
Sbjct: 77  YPVDDGCRIHVIDHSGARL---GEYEDISKVEKYEISQEAYEQRQDSIRSFLKRNKLGRF 136

Query: 141 NP---SAFESKISDTYMEE--LCANIKVGDRCEIE-PGE--KRGVVKFVGRAESLAPGFW 200
           N    +  E++ S   +EE    + I VG RCE+  PG+  +RG V +VG  +   PG+W
Sbjct: 137 NEEERAQQEAENSQRLIEEEAQASTIPVGSRCEVRTPGQPPRRGTVMYVGLTD-FKPGYW 196

Query: 201 VGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           +G++YDEPLGKNDG V G RYF+C   +GA V+P  V V
Sbjct: 197 IGIRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPSVVTV 231

BLAST of MS016969 vs. ExPASy Swiss-Prot
Match: Q99426 (Tubulin-folding cofactor B OS=Homo sapiens OX=9606 GN=TBCB PE=1 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-32
Identity = 89/219 (40.64%), Postives = 121/219 (55.25%), Query Frame = 0

Query: 21  ANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLELYDDSGAKISDLTDNSLPLGF 80
           ++L +F S+ R+S  ++I   K KL    G+  + M LELY       S L      LG 
Sbjct: 17  SSLNTFRSEKRYSRSLTIAEFKCKLELLVGSPASCMELELYGVDDKFYSKLDQEDALLGS 76

Query: 81  YSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEAYDKRDDTFRKF--KEKLASQ 140
           Y   DG R+H+ID   + +   G  ED S VEKY IS+EAYD+R DT R F  + KL   
Sbjct: 77  YPVDDGCRIHVIDHSGARL---GEYEDVSRVEKYTISQEAYDQRQDTVRSFLKRSKLGRY 136

Query: 141 NP---SAFESKISDTYMEE--LCANIKVGDRCEIE---PGEKRGVVKFVGRAESLAPGFW 200
           N    +  E++ +    EE    ++I VG RCE+       +RG V +VG  +   PG+W
Sbjct: 137 NEEERAQQEAEAAQRLAEEKAQASSIPVGSRCEVRAAGQSPRRGTVMYVGLTD-FKPGYW 196

Query: 201 VGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           +GV+YDEPLGKNDG V G RYF+C   +GA V+P  V V
Sbjct: 197 IGVRYDEPLGKNDGSVNGKRYFECQAKYGAFVKPAVVTV 231

BLAST of MS016969 vs. ExPASy TrEMBL
Match: A0A6J1DKD2 (tubulin-folding cofactor B OS=Momordica charantia OX=3673 GN=LOC111021286 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 228/229 (99.56%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL
Sbjct: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA
Sbjct: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCE+EPGEKRGVVKFVG
Sbjct: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEVEPGEKRGVVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 229

BLAST of MS016969 vs. ExPASy TrEMBL
Match: A0A1S3C118 (tubulin-folding cofactor B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495804 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.0e-123
Identity = 218/233 (93.56%), Postives = 227/233 (97.42%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLW+KCGTSVNSMCLEL
Sbjct: 1   MASRLQQIEKDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWRKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSG+KISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEA
Sbjct: 61  YDDSGSKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           YDKRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRC++EPGEKRGVVKFVG
Sbjct: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKVCFAA 234
           RAESLAPGFWVGVQYDEPLGKNDG VKGI YFDC PLHGAMVRPDKVKV F+A
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGTVKGIHYFDCSPLHGAMVRPDKVKVQFSA 233

BLAST of MS016969 vs. ExPASy TrEMBL
Match: A0A6J1HTU0 (tubulin-folding cofactor B OS=Cucurbita maxima OX=3661 GN=LOC111466096 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.9e-122
Identity = 218/229 (95.20%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLWKKCGTSVNSMCLEL
Sbjct: 1   MASRLQQIETDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWKKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSGAKISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA
Sbjct: 61  YDDSGAKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           Y+KRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRCE+EPG+ RG VKFVG
Sbjct: 121 YEKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCEVEPGKIRGSVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
            AESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV
Sbjct: 181 WAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 229

BLAST of MS016969 vs. ExPASy TrEMBL
Match: A0A1S3C1L1 (tubulin-folding cofactor B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495804 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.2e-122
Identity = 216/229 (94.32%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLW+KCGTSVNSMCLEL
Sbjct: 1   MASRLQQIEKDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWRKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSG+KISDLTDNS+PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEA
Sbjct: 61  YDDSGSKISDLTDNSIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           YDKRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRC++EPGEKRGVVKFVG
Sbjct: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESLAPGFWVGVQYDEPLGKNDG VKGI YFDC PLHGAMVRPDKVKV
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGTVKGIHYFDCSPLHGAMVRPDKVKV 229

BLAST of MS016969 vs. ExPASy TrEMBL
Match: A0A0A0L346 (CAP-Gly domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G354120 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.5e-122
Identity = 216/233 (92.70%), Postives = 225/233 (96.57%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MASRLQ+IE DESVLLRVTHANLKSFTSDVRFSLQMS+ESVKEKLW+KCGTSVNSMCLEL
Sbjct: 1   MASRLQQIEKDESVLLRVTHANLKSFTSDVRFSLQMSVESVKEKLWRKCGTSVNSMCLEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSG+KISDLTDN +PLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEK+QISEEA
Sbjct: 61  YDDSGSKISDLTDNCIPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKFQISEEA 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           YDKRDDTFRKFKEKLASQNPSAFESKISD YMEELCANIKVGDRC++EPGEKRGVVKFVG
Sbjct: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDNYMEELCANIKVGDRCQVEPGEKRGVVKFVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKVCFAA 234
           RAESLAPGFWVGVQYDEPLGKNDG VKGI YFDC P HGAMVRPDKVKV F+A
Sbjct: 181 RAESLAPGFWVGVQYDEPLGKNDGTVKGIHYFDCSPFHGAMVRPDKVKVRFSA 233

BLAST of MS016969 vs. TAIR 10
Match: AT3G10220.1 (tubulin folding cofactor B )

HSP 1 Score: 366.3 bits (939), Expect = 1.8e-101
Identity = 171/229 (74.67%), Postives = 201/229 (87.77%), Query Frame = 0

Query: 1   MASRLQEIEADESVLLRVTHANLKSFTSDVRFSLQMSIESVKEKLWKKCGTSVNSMCLEL 60
           MA+   ++E D+SV L +THANLKSF++D RFS QMS+E+VKEKLWKKCGTSVNSM LEL
Sbjct: 1   MATSRLQLEGDDSVHLHITHANLKSFSADARFSPQMSVEAVKEKLWKKCGTSVNSMALEL 60

Query: 61  YDDSGAKISDLTDNSLPLGFYSPLDGYRLHIIDLDPSSVTSGGWLEDTSLVEKYQISEEA 120
           YDDSG+K++ L+D+S PLGF+SP DG+RLHIIDLDPSSVT+GGWLEDTSLVEKY ISEE 
Sbjct: 61  YDDSGSKVAVLSDDSRPLGFFSPFDGFRLHIIDLDPSSVTTGGWLEDTSLVEKYNISEED 120

Query: 121 YDKRDDTFRKFKEKLASQNPSAFESKISDTYMEELCANIKVGDRCEIEPGEKRGVVKFVG 180
           Y KR D+FRKFKEK  SQNP A E+K  + YME+LCANIKVGDRC++EPGEKRG+VK+VG
Sbjct: 121 YAKRTDSFRKFKEKRVSQNPVAAEAKTKENYMEDLCANIKVGDRCQVEPGEKRGMVKYVG 180

Query: 181 RAESLAPGFWVGVQYDEPLGKNDGMVKGIRYFDCPPLHGAMVRPDKVKV 230
           RAESL PG+WVG+QYDEPLGK+DGMVKG R+F+CP L G MVRPDKVKV
Sbjct: 181 RAESLGPGYWVGIQYDEPLGKHDGMVKGTRFFECPRLQGGMVRPDKVKV 229

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153874.12.4e-12799.56tubulin-folding cofactor B [Momordica charantia][more]
XP_008455690.11.0e-12293.56PREDICTED: tubulin-folding cofactor B isoform X1 [Cucumis melo][more]
KAG6595702.11.3e-12295.20Tubulin-folding cofactor B, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023517149.12.3e-12295.20tubulin-folding cofactor B [Cucurbita pepo subsp. pepo][more]
KAG7027664.13.9e-12295.18Tubulin-folding cofactor B [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q67Z522.5e-10074.67Tubulin-folding cofactor B OS=Arabidopsis thaliana OX=3702 GN=TFCB PE=1 SV=1[more]
Q207281.4e-3742.25Tubulin-specific chaperone B OS=Caenorhabditis elegans OX=6239 GN=tbcb-1 PE=1 SV... [more]
Q9D1E66.0e-3340.27Tubulin-folding cofactor B OS=Mus musculus OX=10090 GN=Tbcb PE=1 SV=2[more]
Q5E9517.8e-3340.18Tubulin-folding cofactor B OS=Bos taurus OX=9913 GN=TBCB PE=2 SV=1[more]
Q994261.0e-3240.64Tubulin-folding cofactor B OS=Homo sapiens OX=9606 GN=TBCB PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DKD21.1e-12799.56tubulin-folding cofactor B OS=Momordica charantia OX=3673 GN=LOC111021286 PE=4 S... [more]
A0A1S3C1185.0e-12393.56tubulin-folding cofactor B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495804 PE... [more]
A0A6J1HTU01.9e-12295.20tubulin-folding cofactor B OS=Cucurbita maxima OX=3661 GN=LOC111466096 PE=4 SV=1[more]
A0A1S3C1L14.2e-12294.32tubulin-folding cofactor B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495804 PE... [more]
A0A0A0L3465.5e-12292.70CAP-Gly domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G354120 PE=... [more]
Match NameE-valueIdentityDescription
AT3G10220.11.8e-10174.67tubulin folding cofactor B [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000938CAP Gly-rich domainSMARTSM01052CAP_GLY_2coord: 161..228
e-value: 7.0E-32
score: 121.9
IPR000938CAP Gly-rich domainPFAMPF01302CAP_GLYcoord: 161..227
e-value: 1.3E-23
score: 82.7
IPR000938CAP Gly-rich domainPROSITEPS50245CAP_GLY_2coord: 181..223
score: 17.382498
IPR000626Ubiquitin-like domainPFAMPF14560Ubiquitin_2coord: 14..98
e-value: 8.2E-27
score: 93.5
NoneNo IPR availableGENE3D3.10.20.90coord: 13..97
e-value: 5.5E-30
score: 105.6
NoneNo IPR availablePANTHERPTHR18916:SF76TUBULIN FOLDING COFACTOR Bcoord: 13..229
NoneNo IPR availablePANTHERPTHR18916DYNACTIN 1-RELATED MICROTUBULE-BINDINGcoord: 13..229
NoneNo IPR availableCDDcd01789Ubl_TBCBcoord: 14..93
e-value: 2.14243E-31
score: 108.426
IPR036859CAP Gly-rich domain superfamilyGENE3D2.30.30.190coord: 155..232
e-value: 3.3E-28
score: 99.2
IPR036859CAP Gly-rich domain superfamilySUPERFAMILY74924Cap-Gly domaincoord: 103..229
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 10..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016969.1MS016969.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding