MS016964 (gene) Bitter gourd (TR) v1

Overview
NameMS016964
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPyrroline-5-carboxylate reductase
Locationscaffold197: 28069 .. 32090 (-)
RNA-Seq ExpressionMS016964
SyntenyMS016964
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTCCTTCCCATTTCAATGGATGCTTACAGATTAGGGTTCATCGGAGCTGGAAAAATGGCTGAGAGTATAGCAAGGGGCATTGTCCAATCTGGCCTCTTACCTCCTTCTCGAATCTGTACGGCTGTTCACTCCAACCCTAATCGCCGCATTGCCTTCGAGTCCTTCGGTGTTCGAGTTCTTCCGAGGAACGACAACGTATGATTATTATGATCCACACTTCGTTCTCTGTTTTTTGGCTCCTCGTTTTTCCAGTCTCCGTTCAACTTCGTTCTGTTCCTAACCAGAGCTGTTCTTTTTCTTCTTCTTTTTTGCTTATGATTGCTTATTATCAGGTAGTTGAAGAGAGTGATGTGGTTATTTTCTCTGTTAAACCTCAAGTTGGTACGTTCTCATTTATTGCTGCCCTATTTTCCCCATTTATTTATTCATTCACATTATCTTCTTGAGAAATGTTTGAAAGGATTAGTAACAATATCTCTGGAGTCTGGATGATTTGAAAGAAGCCCTGGTGATTTGTATCTCTTGTTGTTGTTTTTTTTTGGCCCTCTCAACATGCAGGATTGTTATGCTTTCTTGTTTGATTTATTGGGTTTATAATGAACGTCGTTTGAAGAACTCGTGCTTAAGAAGTTTGTTTTCTCCATCAATTTAAGTCTGCGAACTTTAAATTGTTTCTGACAGCAGCGCTCCTGGCAAAGACTTGAGATCTCTTAGTCATACCAGCTTAGAGGTCATGAATTCGAACCTTAGGATGTGCTTAATAACAAAAACCTTTGACGTAACTCCAGTCCAGGCCTTGGGACGGACACGGGTGCCCCTTGATATCGGGGAGCAAAGCTCCAACTCTCGGTTATAAAAAAAAAAAATCTGACAGCTTATATTGGTTGCAGTTCAGAATGTGGTGTTGAAGCTGAGGCCTCTGCTCACGGGAAAGAAGCTTTTGGTTTCAGTTGCTGCTGGGGTGAAATTGAAGGATCTGCAGGTTCTTGATTTCAATAACTTGTTCTTTTCTTTGGACTTCCAGCTGGATGAGATCTTTTTAAGTTGAGTGTTGTGTGGGAAAATAGTAGCCACCGGGATTATAATGCAAATGGCTTCACTTATCATGTCATATTTGGGGCTTCTCTTTAAATATTTTGCTAGAAATCTATGGAATGAAGCAGTTTTTGCAATCTGCTTTTACTTTTACTGTGTACTAAGTAAAGGGACGTTAATGCTTTTATAGTCTCCTCCATTTGCTAAAAAGTTTCAACTTAATCACATTATTATTCTCTGGATTTTTCCTTTTCTACATACAAATTGCACTTGAAGTAATGCTTTATACATTTAGGATTGGGCAGGTCATAACCGATTTATTAGAGTTATGCCAAATACTCCAGCTGCTGTTGGTGAGGCTGCATCAGGTATTATCTTTTCCTCTTATGTTTAGTGTCATTCTACACCCCAAATCTGTTTCCAGACGGCTGAAATAGGTGCTACCTGTTAGAATGTGAAAAAAGATATATCATATAGGCATCTTAGAACTCTTAAGATATCAAATAAAAACAAAATAAAAAATAAATGGTAAATGGTAAATGGTAAATGGTAAATAGGTAACAGTATTTCTTTTAACAAAAATAATGAAAATAATTTAAGGATTAGGGTTAATTAAGAGGGAATGGATTCAAACTCCATGATGACTACCTGCCATAGGAATTACTAATTCTTTTGTGTTTCCCGGCACCAAAAAAAAAAAAAAAATTAAGGATGAAGTTCTATTTTTTGGTCTCCTTTTACCTAGATAATGCAGCTTCATGAGATTTGAAGCTTTTTCTCTTCAAATATATCCATGTCTGTGCAGAATTGGGTTTCTTTAACTTTCTGGTTGAGAATTTGGTTTACTTCTTATCAAGAAAAAGAATTTGGTTTGCTTACTCAAAAGCTTCATCCAAGCCAGATCAAGCTTGCAAAATAAATTCATTAACTTCATTACATGATGTAGAAGAAACATTTTGAGATTTCCAATTAATCTTCGTGCTCGGTGCATTGCATGGAAAGTACAAAAATGAACTAATAAAAAATGTCTATTCATGTTAAAAAAAAAAGAATGAAAAATTCTGTAATTTACTTGTGTTTTACTAGTTATGAGCTTGGGAGAAGGTGCAACAAAGGAGGATGGCCATATAATAGCTAAATTATTTGGATCTGTGGGTAAGATATGGGAAGCTGATGAGAAGTTATTTGATGCAATCACTGGTCTGAGGTACGTAACATGTTGTGAGAGATGAATCCCACACTTCCAAGTTAGTAGAGCTTAATACCCAAAGTTCTCTTCTATCTGCTGGCTCTTAATTATTTTAGGAGCATTCTTTTGTTGGGTATACAATTAAGATAAATGTTAGGATATTATTAGAATTTTTTTGTTAGGATATTGGGATATTATTAGAGTATTTTGAGCATATTAGTCATTAGCTAGGAGTTTGTTAGAGAACTGTTTATAAATAAAATAATTAGAGACGAGCAGATTTTTGAGATAGTCTTTGGGAGATAACTAGGCCTCTTGAATGGTTAGTAAACCTTTGTAATTTTCTTTCTAAATATCAACAGCATATTTCTATTAAATTTCTTGTCAATTTTTAGTTCCTATCCGCATTCTGCTGCTAATAGATACTCGTAAATGTAAATGGAAATATGCAGTGGTAGTGGGCCAGCATACATATTCCTGGCAATTGAAGCTTTGGCTGATGGAGGAGTTGCTGCAGGTCTACCACGAGAACTTGCAATGGGTCTAGCTTCTCAAACTGTGAGTCAAATTCATATGCTTTATTTTTATGGGTAAAGTAGTTAATCCTGTATACGATATAGGCATACCGCGACTGCACTGAAGAAACTAGTAATAGAAATACCCTTTCTTCCATGATGCGAGGTGACTCTAAGGCAACAAATTTATCGCTTGACTAAAAGCAAGAGATTCAAAAATAGCATCACCCCAGTGAAGAATGAAGATTATAGACTCCTTTCATTAAAAAAAAAAGAAAAGAAACATTATGCATACTTGTTTGCACATTTTATAACATTGGAGACCATAAATAACAGTATAGCTGTCCTGTCATATCTTAGCATTTTGTTTTACAATGTGAATACTATGATCTGAGAAATGATCAATCTTGCTGAGCCTTAAAGAAGAAAAAGATCAAACTTGCCCTTTGATGTCAAGTAAAAGGAGATAAAAAAAACATTCCTAAAATGAAAAGTAGCTGAAACTATAATGATATGATGCTTGCGTCTGAACTTTGTACCGTCTGAAAGATGCCATGTAGAATATGAAGATGATAAACGGCAGGTTGTGTAAGGATTTTATTGAAATTTATAGACCTTTATCTTCACTAAAAATCAATCACTAGTCATTAACCCATCTGGTTTCCCTATACAATGAACTCTAGGGTGGTATGCGAAGGACGATATTAGGAGGTCGTTATGGTGACTGATGCACATTCCGAAATTTAGCACTTAGATGTGTAGTGTCTCATCTAATGCAAATAGAAGTCTAAAGTTGTACATTCTTCTTCCCCTTGCTGTATTAATTGACAAATCTGATCCACAAATCAAAGTCCACTTGTTATTGCTTTGCTCATAAAGTGGAAAAAGAAATTCTCTGCCATGTATACATCACATCTAGGATTCCCAATCCTTGTTTGGATCTTGTTACCTTTGCAATACCAATCAGTTTTTCAGTACAAAAAACAAAGACCTTGAAGTCAAACTATGATCATCTAGCAAGGAAAATCGGAATCAGTATTTATACTCTGATTTTATCAGTTGGAACTAACTGATTCAGAATTTGTTTACAGGTGTTAGGAGCGGCCTCCATGGTCACCAAAACTGGGAAGCACCCTGGACAGCTCAAAGATGATGTTACATCGCCTGGTGGGACTACCATTGCTGGTATCCATGAATTAGAGAAGGGAGGACTCCGCGGCATGTTTATGAACGCTGTCGTTGCTGCTGCTAAGCGGAGCAAAGAACTTTCCCCAAAG

mRNA sequence

ATGGAGGTCCTTCCCATTTCAATGGATGCTTACAGATTAGGGTTCATCGGAGCTGGAAAAATGGCTGAGAGTATAGCAAGGGGCATTGTCCAATCTGGCCTCTTACCTCCTTCTCGAATCTGTACGGCTGTTCACTCCAACCCTAATCGCCGCATTGCCTTCGAGTCCTTCGGTGTTCGAGTTCTTCCGAGGAACGACAACGTAGTTGAAGAGAGTGATGTGGTTATTTTCTCTGTTAAACCTCAAGTTGTTCAGAATGTGGTGTTGAAGCTGAGGCCTCTGCTCACGGGAAAGAAGCTTTTGGTTTCAGTTGCTGCTGGGGTGAAATTGAAGGATCTGCAGGATTGGGCAGGTCATAACCGATTTATTAGAGTTATGCCAAATACTCCAGCTGCTGTTGGTGAGGCTGCATCAGTTATGAGCTTGGGAGAAGGTGCAACAAAGGAGGATGGCCATATAATAGCTAAATTATTTGGATCTGTGGGTAAGATATGGGAAGCTGATGAGAAGTTATTTGATGCAATCACTGGTCTGAGTGGTAGTGGGCCAGCATACATATTCCTGGCAATTGAAGCTTTGGCTGATGGAGGAGTTGCTGCAGGTCTACCACGAGAACTTGCAATGGGTCTAGCTTCTCAAACTGTGTTAGGAGCGGCCTCCATGGTCACCAAAACTGGGAAGCACCCTGGACAGCTCAAAGATGATGTTACATCGCCTGGTGGGACTACCATTGCTGGTATCCATGAATTAGAGAAGGGAGGACTCCGCGGCATGTTTATGAACGCTGTCGTTGCTGCTGCTAAGCGGAGCAAAGAACTTTCCCCAAAG

Coding sequence (CDS)

ATGGAGGTCCTTCCCATTTCAATGGATGCTTACAGATTAGGGTTCATCGGAGCTGGAAAAATGGCTGAGAGTATAGCAAGGGGCATTGTCCAATCTGGCCTCTTACCTCCTTCTCGAATCTGTACGGCTGTTCACTCCAACCCTAATCGCCGCATTGCCTTCGAGTCCTTCGGTGTTCGAGTTCTTCCGAGGAACGACAACGTAGTTGAAGAGAGTGATGTGGTTATTTTCTCTGTTAAACCTCAAGTTGTTCAGAATGTGGTGTTGAAGCTGAGGCCTCTGCTCACGGGAAAGAAGCTTTTGGTTTCAGTTGCTGCTGGGGTGAAATTGAAGGATCTGCAGGATTGGGCAGGTCATAACCGATTTATTAGAGTTATGCCAAATACTCCAGCTGCTGTTGGTGAGGCTGCATCAGTTATGAGCTTGGGAGAAGGTGCAACAAAGGAGGATGGCCATATAATAGCTAAATTATTTGGATCTGTGGGTAAGATATGGGAAGCTGATGAGAAGTTATTTGATGCAATCACTGGTCTGAGTGGTAGTGGGCCAGCATACATATTCCTGGCAATTGAAGCTTTGGCTGATGGAGGAGTTGCTGCAGGTCTACCACGAGAACTTGCAATGGGTCTAGCTTCTCAAACTGTGTTAGGAGCGGCCTCCATGGTCACCAAAACTGGGAAGCACCCTGGACAGCTCAAAGATGATGTTACATCGCCTGGTGGGACTACCATTGCTGGTATCCATGAATTAGAGAAGGGAGGACTCCGCGGCATGTTTATGAACGCTGTCGTTGCTGCTGCTAAGCGGAGCAAAGAACTTTCCCCAAAG

Protein sequence

MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVRVLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHNRFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSGSGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPGGTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK
Homology
BLAST of MS016964 vs. NCBI nr
Match: XP_022153943.1 (pyrroline-5-carboxylate reductase [Momordica charantia])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR
Sbjct: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 276

BLAST of MS016964 vs. NCBI nr
Match: XP_038881291.1 (pyrroline-5-carboxylate reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 505.4 bits (1300), Expect = 3.2e-139
Identity = 260/276 (94.20%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPIS+DAYRLGFIGAGKMAESIA+GIVQSGLLPPSRI TAVHSNP+RRIAFESFGV+
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVQ 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDVVI SVKPQVV+NVVLKLRPLL+GKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQVVKNVVLKLRPLLSGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGA KEDG ++AKLFGSVGKIWEADEK FDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKIWEADEKFFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKRS++LSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSQDLSPK 276

BLAST of MS016964 vs. NCBI nr
Match: XP_004142647.1 (pyrroline-5-carboxylate reductase [Cucumis sativus] >KGN54523.1 hypothetical protein Csa_012959 [Cucumis sativus])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 259/276 (93.84%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPIS+DAYRLGFIGAGKMAESIA+GIVQSGLLPPSRI TAVHSNP+RRIAFESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDVVI SVKPQVV+NVVLKLRPLL+GKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQVVKNVVLKLRPLLSGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGA KEDG ++AKLFGSVGKIWEA+EK FDA+TGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKIWEAEEKYFDAVTGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +ELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of MS016964 vs. NCBI nr
Match: XP_008455688.1 (PREDICTED: pyrroline-5-carboxylate reductase [Cucumis melo] >KAA0062733.1 pyrroline-5-carboxylate reductase [Cucumis melo var. makuwa] >TYK22531.1 pyrroline-5-carboxylate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 259/276 (93.84%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPIS+DAYRLGFIGAGKMAESIA+GIVQSGLLPPSRI TAVHSNP+RRIAFESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDVVI SVKPQVV+NVVLKLRPLL+GKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQVVKNVVLKLRPLLSGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RF+RVMPNTPAAVGEAASVMSLGEGA KEDG ++AKLFGSVGKIWEA+EK FDAITGLSG
Sbjct: 121 RFVRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKIWEAEEKYFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +ELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of MS016964 vs. NCBI nr
Match: XP_022925170.1 (pyrroline-5-carboxylate reductase-like [Cucurbita moschata] >KAG6595699.1 Pyrroline-5-carboxylate reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027661.1 Pyrroline-5-carboxylate reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.5 bits (1264), Expect = 4.7e-135
Identity = 253/276 (91.67%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+LPIS+D+Y LGFIGAGKMAESIA+GIVQSGLLP SRI TAVHSNP+R IAFESFGVR
Sbjct: 1   MEILPISVDSYNLGFIGAGKMAESIAKGIVQSGLLPASRISTAVHSNPSRGIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDV+IFSVKPQVV+NVVLKLRP L+ KKLLVSVAAGVKL+DLQ+WAGHN
Sbjct: 61  VLPKNDNVVEESDVIIFSVKPQVVKNVVLKLRPQLSRKKLLVSVAAGVKLQDLQEWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGA KEDG +IAKLFGSVGKIWEADEKLFDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGALKEDGQLIAKLFGSVGKIWEADEKLFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +EL PK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGRELGPK 276

BLAST of MS016964 vs. ExPASy Swiss-Prot
Match: P17817 (Pyrroline-5-carboxylate reductase OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.1e-117
Identity = 218/274 (79.56%), Postives = 246/274 (89.78%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+ PI  ++Y LGFIGAGKMAESIARG V+SG+LPPSRI TAVH N  RR AFESFGV 
Sbjct: 1   MEIFPIPAESYTLGFIGAGKMAESIARGAVRSGVLPPSRIRTAVHFNLARRGAFESFGVT 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP ND+VV ESDVV+ SVKPQ+V++VV KL PLLT  KLLVSVAAG KLKDLQ+WAG++
Sbjct: 61  VLPSNDDVVRESDVVVLSVKPQLVKDVVSKLTPLLTKHKLLVSVAAGTKLKDLQEWAGND 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVG+AASVMSLG  AT+EDG+IIA+LFGS+GKIW+A+EK FDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGQAASVMSLGGSATEEDGNIIAQLFGSIGKIWKAEEKYFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAY++LAIEALADGGVAAGLPR+L++ LASQTVLGAASMV++TGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYVYLAIEALADGGVAAGLPRDLSLSLASQTVLGAASMVSQTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELS 275
           GTTI GIHELE GG RG  MNAVVAAAKRS+ELS
Sbjct: 241 GTTITGIHELENGGFRGTLMNAVVAAAKRSRELS 274

BLAST of MS016964 vs. ExPASy Swiss-Prot
Match: Q04708 (Pyrroline-5-carboxylate reductase OS=Pisum sativum OX=3888 GN=PROC PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 1.8e-116
Identity = 215/274 (78.47%), Postives = 246/274 (89.78%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+LPI  D+Y LGFIGAGKMAESIA+G  +SG+LP SRI TA HSNP+RR AFES G+ 
Sbjct: 1   MEILPILSDSYTLGFIGAGKMAESIAKGASRSGVLPSSRIVTA-HSNPSRRAAFESIGIT 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VL  ND+VV  S+VV+FSVKPQ+V++VVLKL+PLLT  KLLVSVAAG+KLKDLQ+WAGH 
Sbjct: 61  VLSSNDDVVRASNVVVFSVKPQLVKDVVLKLKPLLTKDKLLVSVAAGIKLKDLQEWAGHE 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVG+AASVMSLG  AT+ED ++I++LFGS+GKIW+AD+K FDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGQAASVMSLGGAATEEDANLISQLFGSIGKIWKADDKFFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYI+LAIEALADGGVAAGLPR+LA+ LASQTVLGAASM T +GKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATLSGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELS 275
           GTTIAG+HELEKGG RG  MNAVVAAAKRS+ELS
Sbjct: 241 GTTIAGVHELEKGGFRGTLMNAVVAAAKRSRELS 273

BLAST of MS016964 vs. ExPASy Swiss-Prot
Match: O04016 (Pyrroline-5-carboxylate reductase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc23110 PE=2 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 3.3e-115
Identity = 214/270 (79.26%), Postives = 241/270 (89.26%), Query Frame = 0

Query: 4   LPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVRVLP 63
           +PI  D Y+LGFIGAGKMAESIARG+V+SG+LP SRI TA H    RR AFESFGV+VL 
Sbjct: 7   VPIPTDTYKLGFIGAGKMAESIARGVVKSGVLPASRIRTA-HLGSARRDAFESFGVKVLD 66

Query: 64  RNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHNRFI 123
           RND VVE+SDV+IFSVKPQ+V+ VVL+LRPLL+ K+LLVS+ AGVKLK+L+DWAGH+RFI
Sbjct: 67  RNDQVVEDSDVIIFSVKPQIVKEVVLQLRPLLSEKQLLVSIVAGVKLKELEDWAGHSRFI 126

Query: 124 RVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSGSGP 183
           RVMPNTPAAVGEAASVMSLG  AT EDG +I KLFG++GKIW+ADEKLFDA+TGLSGSGP
Sbjct: 127 RVMPNTPAAVGEAASVMSLGATATGEDGELITKLFGAIGKIWKADEKLFDAVTGLSGSGP 186

Query: 184 AYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPGGTT 243
           AYIFLAIEALADGGVAAGLPRELA+GLASQTVLGAASMV + GKHPGQLKDDV S GGTT
Sbjct: 187 AYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVARGGKHPGQLKDDVASAGGTT 246

Query: 244 IAGIHELEKGGLRGMFMNAVVAAAKRSKEL 274
           IAGIHELEKGG RG  MNAVV+A KRS+E+
Sbjct: 247 IAGIHELEKGGFRGTLMNAVVSATKRSQEI 275

BLAST of MS016964 vs. ExPASy Swiss-Prot
Match: P54904 (Pyrroline-5-carboxylate reductase OS=Arabidopsis thaliana OX=3702 GN=PROC1 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 9.7e-115
Identity = 211/274 (77.01%), Postives = 243/274 (88.69%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+LPI  +++++GFIGAGKMAESIARG+V SG+LPP+RICTAVHSN NRR  FESFGV 
Sbjct: 1   MEILPIPAESFKVGFIGAGKMAESIARGVVASGVLPPNRICTAVHSNLNRRDVFESFGVN 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           V   ++ VV+ESDVVIFSVKPQVV+  V +L+  L+  K+LVSVAAG+KL DLQ+W+G +
Sbjct: 61  VFSTSEEVVKESDVVIFSVKPQVVKKAVTELKSKLSKNKILVSVAAGIKLNDLQEWSGQD 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLG GAT+EDG I+A LFG+VGKI +ADEK+FDA+TGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKILKADEKMFDAVTGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELA+ LASQTVLGAA+MV+KTGKHPG LKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELALSLASQTVLGAATMVSKTGKHPGVLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELS 275
           GTTIAG+HELEKG  R   MNAVVAAAKRS+ELS
Sbjct: 241 GTTIAGVHELEKGSFRATLMNAVVAAAKRSRELS 274

BLAST of MS016964 vs. ExPASy Swiss-Prot
Match: Q9HH99 (Pyrroline-5-carboxylate reductase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=proC PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 5.6e-62
Identity = 129/270 (47.78%), Postives = 179/270 (66.30%), Query Frame = 0

Query: 8   MDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVRVLPRNDN 67
           M+  ++GFIGAGKM  ++ +G +++G++ P  I  +    P  +      G+RV   N  
Sbjct: 1   MENQKIGFIGAGKMGSALMQGTIKAGIVTPENIGASDVYEPFLKDLQAKLGIRVSTDNAV 60

Query: 68  VVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQD-WAGHNRFIRVM 127
           +V ESD++I +VKPQ + +V+  L+  +T +KL++S+AAGV L   +D      R +RVM
Sbjct: 61  IVRESDILILAVKPQTLSSVLSNLKNEITSEKLVISIAAGVPLSTYEDALLEGTRVVRVM 120

Query: 128 PNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSGSGPAYI 187
           PN  A V EAAS ++ G+ AT ED     ++F +VG   +  E L DA+TGLSGSGPA+I
Sbjct: 121 PNIAATVSEAASGIAPGKNATPEDLKAALEIFSAVGTAVQVPESLMDAVTGLSGSGPAFI 180

Query: 188 FLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPGGTTIAG 247
           F  IEA+ADG V  G+ R+ A+ LA+QTVLGAA M  +TG HPG+LKD VTSP GTTI G
Sbjct: 181 FPVIEAMADGAVLEGMDRKSALTLAAQTVLGAAKMALETGMHPGELKDMVTSPAGTTIQG 240

Query: 248 IHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           IH LE+ G+R  FMNAV+ A++RSKEL  K
Sbjct: 241 IHSLEEAGIRAAFMNAVIRASERSKELGKK 270

BLAST of MS016964 vs. ExPASy TrEMBL
Match: A0A6J1DIW6 (Pyrroline-5-carboxylate reductase OS=Momordica charantia OX=3673 GN=LOC111021333 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.0e-148
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR
Sbjct: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 276

BLAST of MS016964 vs. ExPASy TrEMBL
Match: A0A5D3DFQ4 (Pyrroline-5-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold523G00130 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.4e-139
Identity = 259/276 (93.84%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPIS+DAYRLGFIGAGKMAESIA+GIVQSGLLPPSRI TAVHSNP+RRIAFESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDVVI SVKPQVV+NVVLKLRPLL+GKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQVVKNVVLKLRPLLSGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RF+RVMPNTPAAVGEAASVMSLGEGA KEDG ++AKLFGSVGKIWEA+EK FDAITGLSG
Sbjct: 121 RFVRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKIWEAEEKYFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +ELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of MS016964 vs. ExPASy TrEMBL
Match: A0A0A0KYH5 (Pyrroline-5-carboxylate reductase OS=Cucumis sativus OX=3659 GN=Csa_4G354630 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.4e-139
Identity = 259/276 (93.84%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPIS+DAYRLGFIGAGKMAESIA+GIVQSGLLPPSRI TAVHSNP+RRIAFESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDVVI SVKPQVV+NVVLKLRPLL+GKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQVVKNVVLKLRPLLSGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGA KEDG ++AKLFGSVGKIWEA+EK FDA+TGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKIWEAEEKYFDAVTGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +ELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of MS016964 vs. ExPASy TrEMBL
Match: A0A1S3C257 (Pyrroline-5-carboxylate reductase OS=Cucumis melo OX=3656 GN=LOC103495803 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.4e-139
Identity = 259/276 (93.84%), Postives = 270/276 (97.83%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           MEVLPIS+DAYRLGFIGAGKMAESIA+GIVQSGLLPPSRI TAVHSNP+RRIAFESFGVR
Sbjct: 1   MEVLPISIDAYRLGFIGAGKMAESIAKGIVQSGLLPPSRISTAVHSNPSRRIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDVVI SVKPQVV+NVVLKLRPLL+GKKLLVSVAAGVKLKDLQDWAGHN
Sbjct: 61  VLPKNDNVVEESDVVILSVKPQVVKNVVLKLRPLLSGKKLLVSVAAGVKLKDLQDWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RF+RVMPNTPAAVGEAASVMSLGEGA KEDG ++AKLFGSVGKIWEA+EK FDAITGLSG
Sbjct: 121 RFVRVMPNTPAAVGEAASVMSLGEGAIKEDGQLVAKLFGSVGKIWEAEEKYFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +ELSPK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGQELSPK 276

BLAST of MS016964 vs. ExPASy TrEMBL
Match: A0A6J1EH59 (Pyrroline-5-carboxylate reductase OS=Cucurbita moschata OX=3662 GN=LOC111432490 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.3e-135
Identity = 253/276 (91.67%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+LPIS+D+Y LGFIGAGKMAESIA+GIVQSGLLP SRI TAVHSNP+R IAFESFGVR
Sbjct: 1   MEILPISVDSYNLGFIGAGKMAESIAKGIVQSGLLPASRISTAVHSNPSRGIAFESFGVR 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           VLP+NDNVVEESDV+IFSVKPQVV+NVVLKLRP L+ KKLLVSVAAGVKL+DLQ+WAGHN
Sbjct: 61  VLPKNDNVVEESDVIIFSVKPQVVKNVVLKLRPQLSRKKLLVSVAAGVKLQDLQEWAGHN 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLGEGA KEDG +IAKLFGSVGKIWEADEKLFDAITGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGEGALKEDGQLIAKLFGSVGKIWEADEKLFDAITGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELSPK 277
           GTTIAGIHELEKGGLRGMFMNAVVAAAKR +EL PK
Sbjct: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRGRELGPK 276

BLAST of MS016964 vs. TAIR 10
Match: AT5G14800.1 (pyrroline-5- carboxylate (P5C) reductase )

HSP 1 Score: 414.5 bits (1064), Expect = 6.9e-116
Identity = 211/274 (77.01%), Postives = 243/274 (88.69%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+LPI  +++++GFIGAGKMAESIARG+V SG+LPP+RICTAVHSN NRR  FESFGV 
Sbjct: 1   MEILPIPAESFKVGFIGAGKMAESIARGVVASGVLPPNRICTAVHSNLNRRDVFESFGVN 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           V   ++ VV+ESDVVIFSVKPQVV+  V +L+  L+  K+LVSVAAG+KL DLQ+W+G +
Sbjct: 61  VFSTSEEVVKESDVVIFSVKPQVVKKAVTELKSKLSKNKILVSVAAGIKLNDLQEWSGQD 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLG GAT+EDG I+A LFG+VGKI +ADEK+FDA+TGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKILKADEKMFDAVTGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTVLGAASMVTKTGKHPGQLKDDVTSPG 240
           SGPAYIFLAIEALADGGVAAGLPRELA+ LASQTVLGAA+MV+KTGKHPG LKDDVTSPG
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELALSLASQTVLGAATMVSKTGKHPGVLKDDVTSPG 240

Query: 241 GTTIAGIHELEKGGLRGMFMNAVVAAAKRSKELS 275
           GTTIAG+HELEKG  R   MNAVVAAAKRS+ELS
Sbjct: 241 GTTIAGVHELEKGSFRATLMNAVVAAAKRSRELS 274

BLAST of MS016964 vs. TAIR 10
Match: AT5G14800.2 (pyrroline-5- carboxylate (P5C) reductase )

HSP 1 Score: 319.7 bits (818), Expect = 2.3e-87
Identity = 162/215 (75.35%), Postives = 190/215 (88.37%), Query Frame = 0

Query: 1   MEVLPISMDAYRLGFIGAGKMAESIARGIVQSGLLPPSRICTAVHSNPNRRIAFESFGVR 60
           ME+LPI  +++++GFIGAGKMAESIARG+V SG+LPP+RICTAVHSN NRR  FESFGV 
Sbjct: 1   MEILPIPAESFKVGFIGAGKMAESIARGVVASGVLPPNRICTAVHSNLNRRDVFESFGVN 60

Query: 61  VLPRNDNVVEESDVVIFSVKPQVVQNVVLKLRPLLTGKKLLVSVAAGVKLKDLQDWAGHN 120
           V   ++ VV+ESDVVIFSVKPQVV+  V +L+  L+  K+LVSVAAG+KL DLQ+W+G +
Sbjct: 61  VFSTSEEVVKESDVVIFSVKPQVVKKAVTELKSKLSKNKILVSVAAGIKLNDLQEWSGQD 120

Query: 121 RFIRVMPNTPAAVGEAASVMSLGEGATKEDGHIIAKLFGSVGKIWEADEKLFDAITGLSG 180
           RFIRVMPNTPAAVGEAASVMSLG GAT+EDG I+A LFG+VGKI +ADEK+FDA+TGLSG
Sbjct: 121 RFIRVMPNTPAAVGEAASVMSLGTGATEEDGAIVAMLFGAVGKILKADEKMFDAVTGLSG 180

Query: 181 SGPAYIFLAIEALADGGVAAGLPRELAMGLASQTV 216
           SGPAYIFLAIEALADGGVAAGLPRELA+ LASQTV
Sbjct: 181 SGPAYIFLAIEALADGGVAAGLPRELALSLASQTV 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153943.11.9e-147100.00pyrroline-5-carboxylate reductase [Momordica charantia][more]
XP_038881291.13.2e-13994.20pyrroline-5-carboxylate reductase isoform X1 [Benincasa hispida][more]
XP_004142647.17.1e-13993.84pyrroline-5-carboxylate reductase [Cucumis sativus] >KGN54523.1 hypothetical pro... [more]
XP_008455688.17.1e-13993.84PREDICTED: pyrroline-5-carboxylate reductase [Cucumis melo] >KAA0062733.1 pyrrol... [more]
XP_022925170.14.7e-13591.67pyrroline-5-carboxylate reductase-like [Cucurbita moschata] >KAG6595699.1 Pyrrol... [more]
Match NameE-valueIdentityDescription
P178172.1e-11779.56Pyrroline-5-carboxylate reductase OS=Glycine max OX=3847 PE=2 SV=1[more]
Q047081.8e-11678.47Pyrroline-5-carboxylate reductase OS=Pisum sativum OX=3888 GN=PROC PE=2 SV=1[more]
O040163.3e-11579.26Pyrroline-5-carboxylate reductase OS=Actinidia chinensis var. chinensis OX=15908... [more]
P549049.7e-11577.01Pyrroline-5-carboxylate reductase OS=Arabidopsis thaliana OX=3702 GN=PROC1 PE=2 ... [more]
Q9HH995.6e-6247.78Pyrroline-5-carboxylate reductase OS=Methanosarcina acetivorans (strain ATCC 353... [more]
Match NameE-valueIdentityDescription
A0A6J1DIW69.0e-148100.00Pyrroline-5-carboxylate reductase OS=Momordica charantia OX=3673 GN=LOC111021333... [more]
A0A5D3DFQ43.4e-13993.84Pyrroline-5-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0KYH53.4e-13993.84Pyrroline-5-carboxylate reductase OS=Cucumis sativus OX=3659 GN=Csa_4G354630 PE=... [more]
A0A1S3C2573.4e-13993.84Pyrroline-5-carboxylate reductase OS=Cucumis melo OX=3656 GN=LOC103495803 PE=3 S... [more]
A0A6J1EH592.3e-13591.67Pyrroline-5-carboxylate reductase OS=Cucurbita moschata OX=3662 GN=LOC111432490 ... [more]
Match NameE-valueIdentityDescription
AT5G14800.16.9e-11677.01pyrroline-5- carboxylate (P5C) reductase [more]
AT5G14800.22.3e-8775.35pyrroline-5- carboxylate (P5C) reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029036Pyrroline-5-carboxylate reductase, dimerisation domainPFAMPF14748P5CR_dimercoord: 169..273
e-value: 7.5E-43
score: 145.1
IPR000304Pyrroline-5-carboxylate reductaseTIGRFAMTIGR00112TIGR00112coord: 13..273
e-value: 1.5E-89
score: 298.1
IPR000304Pyrroline-5-carboxylate reductasePIRSFPIRSF000193P5CRcoord: 10..276
e-value: 2.2E-91
score: 303.5
IPR000304Pyrroline-5-carboxylate reductasePANTHERPTHR11645PYRROLINE-5-CARBOXYLATE REDUCTASEcoord: 8..274
IPR000304Pyrroline-5-carboxylate reductasePROSITEPS00521P5CRcoord: 229..251
IPR000304Pyrroline-5-carboxylate reductaseHAMAPMF_01925P5C_reductasecoord: 11..273
score: 27.366169
IPR028939Pyrroline-5-carboxylate reductase, catalytic, N-terminalPFAMPF03807F420_oxidoredcoord: 12..107
e-value: 1.2E-18
score: 67.5
NoneNo IPR availableGENE3D1.10.3730.10coord: 172..275
e-value: 6.5E-45
score: 153.9
NoneNo IPR availableGENE3D3.40.50.720coord: 1..171
e-value: 2.8E-61
score: 208.1
NoneNo IPR availablePANTHERPTHR11645:SF0PYRROLINE-5-CARBOXYLATE REDUCTASE 2coord: 8..274
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 12..166
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 168..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016964.1MS016964.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006561 proline biosynthetic process
molecular_function GO:0004735 pyrroline-5-carboxylate reductase activity