Homology
BLAST of MS016963 vs. NCBI nr
Match:
XP_022153905.1 (triacylglycerol lipase SDP1 [Momordica charantia])
HSP 1 Score: 1678.7 bits (4346), Expect = 0.0e+00
Identity = 845/851 (99.29%), Postives = 847/851 (99.53%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL
Sbjct: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQI KLIKEYINEVSTQLRLVCDSDSEELLLEEKLS MHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQISKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSXMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFFDDSWHSLQFFDQMG
Sbjct: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFDDSWHSLQFFDQMG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLFEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600
Query: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660
SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS
Sbjct: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660
Query: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720
ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS
Sbjct: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720
Query: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780
PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS
Sbjct: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780
Query: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNSEQ 840
HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPK NPVNNSEQ
Sbjct: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKRNPVNNSEQ 840
Query: 841 QDGRDQVFMDT 852
QDGRDQVFMDT
Sbjct: 841 QDGRDQVFMDT 851
BLAST of MS016963 vs. NCBI nr
Match:
XP_038881740.1 (triacylglycerol lipase SDP1 [Benincasa hispida])
HSP 1 Score: 1558.5 bits (4034), Expect = 0.0e+00
Identity = 789/854 (92.39%), Postives = 816/854 (95.55%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
M+ISNEASVGSFSIGPSTI+GRT+AFRILFCKSV QLR Q+FR+LLNIIYRF+ALV PIL
Sbjct: 1 MEISNEASVGSFSIGPSTIIGRTVAFRILFCKSVLQLRHQLFRVLLNIIYRFKALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNL PEEG G+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLAPEEGPGTSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQILELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQILELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAER--AAAAAAASHSPSIPVKF 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAER AAAAAAAS+ P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAAAASYGPVIPVKF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
SASRRIPSWNCIARENSTGSLEEEYLAD+ STHHQG+GGS GAGSSGR+ RTHR MFDGS
Sbjct: 601 SASRRIPSWNCIARENSTGSLEEEYLADITSTHHQGVGGSTGAGSSGRLLRTHRGMFDGS 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDD NRGLVANS+ Q++GGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDFNRGLVANSNVLQTIGGSQNS 720
Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
QSPRTMSERSS+SP+FD R+LSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPEFDARELSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780
Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
RS DSETQNSEVECLQIDCSEK IDASS SD GNEE ATP G QE PSPKSNPVN+S
Sbjct: 781 RSQDSETQNSEVECLQIDCSEKYIDASSASDYDGNEEDATPEGVLQE--PSPKSNPVNHS 840
Query: 841 EQQ-DGRDQVFMDT 852
EQQ DG D + +D+
Sbjct: 841 EQQLDGHDHISIDS 852
BLAST of MS016963 vs. NCBI nr
Match:
XP_008457586.1 (PREDICTED: triacylglycerol lipase SDP1 [Cucumis melo])
HSP 1 Score: 1548.9 bits (4009), Expect = 0.0e+00
Identity = 786/854 (92.04%), Postives = 814/854 (95.32%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRFRALV PIL
Sbjct: 1 MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRYQLFRVLLNVIYRFRALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
SASRRIPSWNCIARENSTGSLEEEYLAD+ TH+QG+GGSIG GSSGRM RTHRNMFDGS
Sbjct: 601 SASRRIPSWNCIARENSTGSLEEEYLADITLTHNQGVGGSIGTGSSGRMLRTHRNMFDGS 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+ Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVLQTVGGSQNS 720
Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
QSPRTMSERSS+SPDFD R+LSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDPRELSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780
Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
RSHDSETQNSEVECLQ+D SE+ +DASS SD GNEE ATP G QE + +N VN S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESLTI--NNLVNRS 840
Query: 841 -EQQDGRDQVFMDT 852
EQQDG D + MD+
Sbjct: 841 EEQQDGHDHISMDS 852
BLAST of MS016963 vs. NCBI nr
Match:
XP_004142709.1 (triacylglycerol lipase SDP1 [Cucumis sativus] >KGN54525.1 hypothetical protein Csa_012774 [Cucumis sativus])
HSP 1 Score: 1543.1 bits (3994), Expect = 0.0e+00
Identity = 781/854 (91.45%), Postives = 815/854 (95.43%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRF+ALV PIL
Sbjct: 1 MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRHQLFRVLLNVIYRFKALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP++NESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKLNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
SA+RRIPSWNCIARENSTGSLEEEYL D+ TH+QG GGSIGAGSSGRM RTHR++FDGS
Sbjct: 601 SAARRIPSWNCIARENSTGSLEEEYLTDITLTHNQGSGGSIGAGSSGRMLRTHRSIFDGS 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+ Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVVQTVGGSQNS 720
Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
QSPRTMSERSS+SPDFD R+LS RVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDARELSTRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780
Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
RSHDSETQNSEVECLQ+D SE+ +DASS SD GNEE ATP G QE + P +N VN+S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESL--PVNNSVNHS 840
Query: 841 -EQQDGRDQVFMDT 852
EQQDG D++ MD+
Sbjct: 841 EEQQDGHDRISMDS 852
BLAST of MS016963 vs. NCBI nr
Match:
KAG6603329.1 (Triacylglycerol lipase SDP1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 780/857 (91.02%), Postives = 815/857 (95.10%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISN+ASVGSFSIGPSTIVGRT+AFRILFCKSVSQLR Q+FR+LL+IIYR +ALV PIL
Sbjct: 1 MDISNDASVGSFSIGPSTIVGRTVAFRILFCKSVSQLRHQIFRVLLHIIYRLKALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYD ELVRNKLQELR RRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDVELVRNKLQELRRRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIP+LIKEYINEVSTQLRLVCDSDSEELLLEEKL+FMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPRLIKEYINEVSTQLRLVCDSDSEELLLEEKLAFMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVE+KLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVENKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGE+VPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGELVPYHPPFNLDPEEGSGSSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPM+QLKELFNVNHFIVSQANPHIAPLLRMKEF RA+GGNFAAKLAHLVEM
Sbjct: 421 WRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRMKEFIRAYGGNFAAKLAHLVEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGFRLGGIA+LFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFRLGGIARLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA----SHSPSIPV 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA SH PSIPV
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATGTTSHGPSIPV 600
Query: 601 KFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFD 660
KFSASRRIPSWNCIARENSTGSLEEEYL D +THHQG+GGSIGAGSS RM RTHR+MF+
Sbjct: 601 KFSASRRIPSWNCIARENSTGSLEEEYLTDTLTTHHQGVGGSIGAGSSSRMLRTHRSMFE 660
Query: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQ 720
GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLN+GLVANS+ Q VG SQ
Sbjct: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNKGLVANSNVFQPVGSSQ 720
Query: 721 NSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTL 780
NSQSPRTMSERSS+SPDFD R+LSNRVSSSILL EGDFLQPERIPNGIVFN+VKKE+LTL
Sbjct: 721 NSQSPRTMSERSSESPDFDARELSNRVSSSILLNEGDFLQPERIPNGIVFNIVKKEDLTL 780
Query: 781 ASRSHDSETQNSEVECLQIDCSEKDIDASSVSDC-GGNEEYATPHGCFQEPIPSPKSNPV 840
+RSHDSETQNSEVECLQIDCS+K IDASS SD GNEE TP C QE SP+++
Sbjct: 781 TNRSHDSETQNSEVECLQIDCSDKYIDASSASDFDDGNEEDVTPKACLQE--SSPENDLA 840
Query: 841 NNSEQ-QDGRDQVFMDT 852
N+SEQ DGRDQV MD+
Sbjct: 841 NHSEQLHDGRDQVSMDS 855
BLAST of MS016963 vs. ExPASy Swiss-Prot
Match:
Q9LZA6 (Triacylglycerol lipase SDP1 OS=Arabidopsis thaliana OX=3702 GN=SDP1 PE=1 SV=1)
HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 605/823 (73.51%), Postives = 699/823 (84.93%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISNEASV FSIGPS+I+GRTIAFR+LFC+S+SQLR+ +FR LL+ RF+ V P +
Sbjct: 1 MDISNEASVDPFSIGPSSIMGRTIAFRVLFCRSMSQLRRDLFRFLLHWFLRFKLTVSPFV 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW HPRNPQGILA+VTIIAF+LKRYTNVK +AE+AYRRKFWRNMMR+ALTYEEWAHAAKM
Sbjct: 61 SWFHPRNPQGILAVVTIIAFVLKRYTNVKIKAEMAYRRKFWRNMMRTALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
L+KETP+MNES+LYDEELV+NKLQELRHRRQEGSLRDI+F MRADL RNLGNMCN ELHK
Sbjct: 121 LEKETPKMNESDLYDEELVKNKLQELRHRRQEGSLRDIMFCMRADLVRNLGNMCNSELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQ+P+ IKEYI+EVSTQLR+VC+SDSEEL LEEKLSFMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQVPRHIKEYIDEVSTQLRMVCNSDSEELSLEEKLSFMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVV+TLVEHKLLPRIIAGSSVGSI+CAVVA+R+WPELQSFF++S HSLQFFDQ+G
Sbjct: 241 GAFHVGVVRTLVEHKLLPRIIAGSSVGSIICAVVASRSWPELQSFFENSLHSLQFFDQLG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
G+F++V+RVM QGA+H+IRQLQ MLR LTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GVFSIVKRVMTQGALHDIRQLQCMLRNLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGS-GSSAR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+RSGEIVPYHPPFNLDPE G+ SS R
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRSGEIVPYHPPFNLDPEVGTKSSSGR 420
Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
RWRDGSLE+DLPM+QLKELFNVNHFIVSQANPHIAPLLR+K+ RA+GG FAAKLAHLVE
Sbjct: 421 RWRDGSLEVDLPMMQLKELFNVNHFIVSQANPHIAPLLRLKDLVRAYGGRFAAKLAHLVE 480
Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
MEVKHRCNQ+LELGF LGG+AKLFAQ+WEGDVTVVMPATLAQYSKIIQNPTH++LQK+AN
Sbjct: 481 MEVKHRCNQVLELGFPLGGLAKLFAQEWEGDVTVVMPATLAQYSKIIQNPTHVELQKAAN 540
Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAAS-HSPSIPVKF 600
QGRRCTWEKLSAIK+NCGIELALD+ VAILNHMRRLK+SAERAA A ++S H + +F
Sbjct: 541 QGRRCTWEKLSAIKSNCGIELALDDSVAILNHMRRLKKSAERAATATSSSHHGLASTTRF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
+ASRRIPSWN +ARENSTGSL++ L + H SSGR S
Sbjct: 601 NASRRIPSWNVLARENSTGSLDD--LVTDNNLH----------ASSGRNL---------S 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLD-DLNRGLVANSSAHQSVGGSQN 720
DSE+E+++L++WTR+GGPLMRTASANKFIDFVQ+LD+D L RG ++ ++ G
Sbjct: 661 DSETESVELSSWTRTGGPLMRTASANKFIDFVQSLDIDIALVRGFSSSPNSPAVPPGGSF 720
Query: 721 SQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLA 780
+ SPR+++ S + + +L +SSI +TEGD LQPER NG V NVVK+ENL +
Sbjct: 721 TPSPRSIAAHSDIESNSNSNNLGTS-TSSITVTEGDLLQPERTSNGFVLNVVKRENLGMP 780
Query: 781 SRSHDSETQNSEV-ECLQIDCSEKDIDASSVS----DCGGNEE 816
S + QN+E+ E +Q+D EK++D SSVS D NEE
Sbjct: 781 SIGN----QNTELPESVQLDIPEKEMDCSSVSEHEEDDNDNEE 797
BLAST of MS016963 vs. ExPASy Swiss-Prot
Match:
Q9M1I6 (Triacylglycerol lipase SDP1L OS=Arabidopsis thaliana OX=3702 GN=SDP1L PE=1 SV=1)
HSP 1 Score: 1071.2 bits (2769), Expect = 6.0e-312
Identity = 565/824 (68.57%), Postives = 656/824 (79.61%), Query Frame = 0
Query: 1 MDISNEASVGSFS-IGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPI 60
MDISNEA V +FS IGP+TI+GRTIA RILFC SVS R +VFRIL + R L+ P
Sbjct: 1 MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPF 60
Query: 61 LSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAK 120
+S +HPRNPQGIL MVT +AFLL RYT++K +AE+AYRRKFWRNMMR+ALTYEEW+HAAK
Sbjct: 61 VSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAK 120
Query: 121 MLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELH 180
MLDKETP++NE++L+D ELV NKL EL+HRR EGSLRDIIF MRADL RNLGNMCNPELH
Sbjct: 121 MLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELH 180
Query: 181 KGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGAS 240
KGRL +P+LIKEYI+EVSTQLR+VCD D+EEL LEEKLSFMHETRHA+GRTALLLSGGAS
Sbjct: 181 KGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGAS 240
Query: 241 LGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQM 300
LGAFH GVVKTLVEHKLLPRIIAGSSVGS+MCAVV TR+WPELQSFF+ SWH+LQFFDQM
Sbjct: 241 LGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQM 300
Query: 301 GGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPR 360
GGIFT V+RVM QGAVHEIR LQ LR LT+NLTFQEAYD+TGRILGITVCS RKHEPPR
Sbjct: 301 GGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPR 360
Query: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSAR 420
CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+R+GEIVPYHPPFNLDPEEGS +S R
Sbjct: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGS-ASVR 420
Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
RWRDGSLE+DLPMIQLKELFNVNHFIVSQANPHIAP LRMKEF RA GG FAAKLA L E
Sbjct: 421 RWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAE 480
Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
MEVKHRCNQ+LELG L +A LFAQ+WEGDVT+VMPAT +QY KIIQNP+++++QK+AN
Sbjct: 481 MEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAAN 540
Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSP------S 600
QGRRCTWEKL+ IKAN GIELALDECV +LNHMRRLKRSAERAAA +A S SP +
Sbjct: 541 QGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLA 600
Query: 601 IPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRN 660
+F+AS+RIPSWNCIAR+NS+GS++++ LA+ AS +Q I +G+G + +
Sbjct: 601 GTNRFNASKRIPSWNCIARQNSSGSVDDDVLAE-ASRLYQHI--VVGSGRNSNRTSNLSH 660
Query: 661 MFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVG 720
+D + SE ++ + WTRSGGPLMRT SA F D+VQNLD D + +S + S+
Sbjct: 661 TYD-AGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQ---IRASENDSIV 720
Query: 721 GSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEN 780
+ +S S SI +TEGD+LQ R NG V N+V+ EN
Sbjct: 721 AASSS-------------------------SHSITVTEGDYLQTGRTHNGFVLNLVRGEN 780
Query: 781 LTLASRSHDSETQNSEV----ECLQIDCSEKDI--DASSVSDCG 812
L + S DS+ + SE+ E +Q+D EKDI SS S+ G
Sbjct: 781 LRMNSEPEDSQNE-SEIPETPESVQLDSPEKDIIDGESSASEDG 790
BLAST of MS016963 vs. ExPASy Swiss-Prot
Match:
P36165 (Triacylglycerol lipase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGL4 PE=1 SV=1)
HSP 1 Score: 253.1 bits (645), Expect = 1.1e-65
Identity = 165/492 (33.54%), Postives = 262/492 (53.25%), Query Frame = 0
Query: 108 ALTYEEWAHAAKMLDKETPRMN-----ESNLYDEELVRNKLQELRHRRQEGSLRDIIFWM 167
A+++EEW A LD T + ES LYD +L+++ +R R + +++ +
Sbjct: 155 AMSFEEWCSAGARLDDLTGKTEWKQKLESPLYDYKLIKDLTSRMREERLNRNYAQLLYII 214
Query: 168 RADLFRNLGNMCNPELHK-GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMH 227
R + RNLGNM N L++ + LI EY+ E L + +SD ++ L L +
Sbjct: 215 RTNWVRNLGNMGNVNLYRHSHVGTKYLIDEYMMESRLALESLMESDLDDSYL---LGILQ 274
Query: 228 ETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPE 287
+TR GRTAL+LSGG + G FH GV+ TL E LLPR+I+GSS G+I+ ++++ E
Sbjct: 275 QTRRNIGRTALVLSGGGTFGLFHIGVLGTLFELDLLPRVISGSSAGAIVASILSVHHKEE 334
Query: 288 ----LQSFFDDSWHSLQFFDQMG---GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTF 347
L D ++ + Q + + R G + + L + + +LTF
Sbjct: 335 IPVLLNHILDKEFNIFKDDKQKSESENLLIKISRFFKNGTWFDNKHLVNTMIEFLGDLTF 394
Query: 348 QEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKN 407
+EAY+ TG+IL ITV E PR LN LT+P+V+IWSAV ASC+ PG+F + L K+
Sbjct: 395 REAYNRTGKILNITVSPASLFEQPRLLNNLTAPNVLIWSAVCASCSLPGIFPSSPLYEKD 454
Query: 408 -RSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHI 467
++GE P+ +GSS+ ++ DGS++ DLP+ +L E+FNV+H I Q N H+
Sbjct: 455 PKTGERKPW-----------TGSSSVKFVDGSVDNDLPISRLSEMFNVDHIIACQVNIHV 514
Query: 468 APLLRMKEFFRAFGG----NFAAKLAHLVEMEVKHRCNQ---ILELGFRLGGIAK----- 527
P L++ GG F+A+L + N+ ILE+G + GIAK
Sbjct: 515 FPFLKLS--LSCVGGEIEDEFSARLKQNLSSIYNFMANEAIHILEIGSEM-GIAKNALTK 574
Query: 528 ---LFAQDWEGDVTVVMP-ATLAQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAIKANCG 570
+ +Q + GD+T++ L + +++ NPT L + G + TW K+S I+ +CG
Sbjct: 575 LRSVLSQQYSGDITILPDMCMLFRIKELLSNPTKEFLLREITNGAKATWPKVSIIQNHCG 629
BLAST of MS016963 vs. ExPASy Swiss-Prot
Match:
Q12043 (Triacylglycerol lipase 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGL5 PE=1 SV=1)
HSP 1 Score: 238.0 bits (606), Expect = 3.8e-61
Identity = 159/497 (31.99%), Postives = 251/497 (50.50%), Query Frame = 0
Query: 106 RSALTYEEWAHAAKMLD-----KETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIF 165
+ A++Y++W A LD E ++ES+LY+ +L+++ +RH R +++
Sbjct: 53 KHAISYDQWNDIASRLDDLTGLSEWKTIDESSLYNYKLLQDLTIRMRHLRTTHDYHRLLY 112
Query: 166 WMRADLFRNLGNMCNPELHK-GRLQIPKLIKEYINEVSTQL-RLVCDSDSEELLLEEKLS 225
+R RNLGNM N L++ ++I +Y+ E L L+ S+ + L L
Sbjct: 113 LIRTKWVRNLGNMNNVNLYRHSHTGTKQIIHDYLEESQAVLTALIHQSNMNDHYL---LG 172
Query: 226 FMHETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRA 285
+ +TR GRTAL+LSGG++ G FH GV+ L E L+P++I+GSS G+I+ ++
Sbjct: 173 ILQQTRRNIGRTALVLSGGSTFGLFHIGVLAALFESDLMPKVISGSSAGAIVASIFCVHT 232
Query: 286 WPELQS------------FFDDSWHSLQFFDQMGGIFTVVRRVMIQGAVHEIRQLQIMLR 345
E+ S F DD+ S + + R G + L +
Sbjct: 233 TQEIPSLLTNVLNMEFNIFNDDNSKS-----PNENLLIKISRFCQNGTWFNNQPLINTML 292
Query: 346 QLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFE 405
NLTF+EAY+ TG+IL ITV +E P+ LN LT+P+V+IWSAV ASC+ PG+F
Sbjct: 293 SFLGNLTFREAYNKTGKILNITVSPASIYEQPKLLNNLTAPNVLIWSAVCASCSLPGVFP 352
Query: 406 AQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIV 465
+ L K+ + NL S ++ DGS++ D+P+ +L E+FNV+H I
Sbjct: 353 STPLFEKDPHTGKIKEWGATNLH------LSNMKFMDGSVDNDMPISRLSEMFNVDHIIA 412
Query: 466 SQANPHIAPLLRM----------KEFFRAFGGNFAAKLAHLVEMEVKHRCNQILELGFR- 525
Q N H+ PLL+ KE F N K+ E H + + EL F
Sbjct: 413 CQVNIHVFPLLKFSNTCVGGEIEKEITARF-RNQVTKIFKFFSDETIHFLDILKELEFHP 472
Query: 526 --LGGIAKLFAQDWEGDVTVVMPATL-AQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAI 570
+ + LF Q + G+VT++ ++ Q+ ++++NP+ L L G R TW K+S I
Sbjct: 473 YLMTKLKHLFLQQYSGNVTILPDLSMVGQFHEVLKNPSQLFLLHQTTLGARATWPKISMI 532
BLAST of MS016963 vs. ExPASy Swiss-Prot
Match:
Q9Y827 (Triacylglycerol lipase ptl3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ptl3 PE=3 SV=1)
HSP 1 Score: 235.7 bits (600), Expect = 1.9e-60
Identity = 162/489 (33.13%), Postives = 244/489 (49.90%), Query Frame = 0
Query: 105 MRSALTYEEWAHAAKMLD-----KETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDII 164
M A +YE W AA+ LD + ES+ YD LV ++L ELR R + R ++
Sbjct: 10 MEYASSYETWLEAAEKLDVIEGKYQWREQKESDEYDYVLVESRLHELRRHRLSKNTRLLL 69
Query: 165 FWMRADLFRNLGNMCNPELHK-GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLS 224
+R + R+ NM N L+ KLI E+I EV L + ++ +L L+EK++
Sbjct: 70 GLLRNSVARDFANMDNSRLYNYAHSGTKKLIDEFIQEVLMCLTYL--EETPDLSLDEKIT 129
Query: 225 FMHETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRA 284
+ G TAL+LSGG + G H GV+++L E L+P+II GSS G+I+ A R
Sbjct: 130 EFSRLKLTTGNTALILSGGGTFGMTHIGVLQSLHEQGLVPKIICGSSAGAIVACAAAVRN 189
Query: 285 WPELQSFFDDSWHS--LQFFDQMG----GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNL 344
E Q +H+ L F + V++ +G V +I L+ +++ L +
Sbjct: 190 -KEEQEILLRQFHTGDLSVFTDPNAAPPSVIQSVKQYFTRGCVLDISHLERVMKLLIGDF 249
Query: 345 TFQEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMA 404
TFQEAYD +G IL +TV E P LNY+T+P+V++WSAV A+C+ P LF+ L
Sbjct: 250 TFQEAYDRSGYILNVTVSCGSLFEMPSLLNYITAPNVLVWSAVVATCSVPFLFKRATLWE 309
Query: 405 KNRSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPH 464
++ P + + A W DGS++ D+P +L ELF+VNHFIVSQ N H
Sbjct: 310 RD----------PLTREVSAFCVTDAPLWMDGSVDNDIPHAKLTELFHVNHFIVSQVNFH 369
Query: 465 IAPLLRMKEFFRAFGGNFAAKLAHLVEMEVKHRCNQILELGFRLGGIAKL---FAQDWEG 524
I P + M + K L EV ELG KL Q + G
Sbjct: 370 IVPFI-MDPTSHNWVERCCKKAIDLAAQEVSLTFRLFAELGIFSVLFTKLQSVITQKYSG 429
Query: 525 DVTVVMPATLAQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAIKANCGIELALDECVAIL 579
D+T++ + +K+I+NPT L +A +G+R TW K+ + +C IE+ +
Sbjct: 430 DITIIPRLNYREVNKVIKNPTPSFLLDAATRGKRGTWTKVPVTRNHCAIEILIAAA---- 479
BLAST of MS016963 vs. ExPASy TrEMBL
Match:
A0A6J1DI61 (triacylglycerol lipase SDP1 OS=Momordica charantia OX=3673 GN=LOC111021312 PE=4 SV=1)
HSP 1 Score: 1678.7 bits (4346), Expect = 0.0e+00
Identity = 845/851 (99.29%), Postives = 847/851 (99.53%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL
Sbjct: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQI KLIKEYINEVSTQLRLVCDSDSEELLLEEKLS MHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQISKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSXMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFFDDSWHSLQFFDQMG
Sbjct: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFDDSWHSLQFFDQMG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLFEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600
Query: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660
SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS
Sbjct: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660
Query: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720
ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS
Sbjct: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720
Query: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780
PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS
Sbjct: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780
Query: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNSEQ 840
HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPK NPVNNSEQ
Sbjct: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKRNPVNNSEQ 840
Query: 841 QDGRDQVFMDT 852
QDGRDQVFMDT
Sbjct: 841 QDGRDQVFMDT 851
BLAST of MS016963 vs. ExPASy TrEMBL
Match:
A0A1S3C753 (triacylglycerol lipase SDP1 OS=Cucumis melo OX=3656 GN=LOC103497252 PE=4 SV=1)
HSP 1 Score: 1548.9 bits (4009), Expect = 0.0e+00
Identity = 786/854 (92.04%), Postives = 814/854 (95.32%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRFRALV PIL
Sbjct: 1 MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRYQLFRVLLNVIYRFRALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
SASRRIPSWNCIARENSTGSLEEEYLAD+ TH+QG+GGSIG GSSGRM RTHRNMFDGS
Sbjct: 601 SASRRIPSWNCIARENSTGSLEEEYLADITLTHNQGVGGSIGTGSSGRMLRTHRNMFDGS 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+ Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVLQTVGGSQNS 720
Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
QSPRTMSERSS+SPDFD R+LSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDPRELSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780
Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
RSHDSETQNSEVECLQ+D SE+ +DASS SD GNEE ATP G QE + +N VN S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESLTI--NNLVNRS 840
Query: 841 -EQQDGRDQVFMDT 852
EQQDG D + MD+
Sbjct: 841 EEQQDGHDHISMDS 852
BLAST of MS016963 vs. ExPASy TrEMBL
Match:
A0A0A0KY40 (PNPLA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G358630 PE=4 SV=1)
HSP 1 Score: 1543.1 bits (3994), Expect = 0.0e+00
Identity = 781/854 (91.45%), Postives = 815/854 (95.43%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRF+ALV PIL
Sbjct: 1 MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRHQLFRVLLNVIYRFKALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP++NESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKLNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
SA+RRIPSWNCIARENSTGSLEEEYL D+ TH+QG GGSIGAGSSGRM RTHR++FDGS
Sbjct: 601 SAARRIPSWNCIARENSTGSLEEEYLTDITLTHNQGSGGSIGAGSSGRMLRTHRSIFDGS 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+ Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVVQTVGGSQNS 720
Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
QSPRTMSERSS+SPDFD R+LS RVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDARELSTRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780
Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
RSHDSETQNSEVECLQ+D SE+ +DASS SD GNEE ATP G QE + P +N VN+S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESL--PVNNSVNHS 840
Query: 841 -EQQDGRDQVFMDT 852
EQQDG D++ MD+
Sbjct: 841 EEQQDGHDRISMDS 852
BLAST of MS016963 vs. ExPASy TrEMBL
Match:
A0A6J1HVX3 (triacylglycerol lipase SDP1-like OS=Cucurbita maxima OX=3661 GN=LOC111467158 PE=4 SV=1)
HSP 1 Score: 1531.2 bits (3963), Expect = 0.0e+00
Identity = 772/856 (90.19%), Postives = 810/856 (94.63%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISN+ASVGSFSIGPSTIVGRT+AFRILFCKSVSQLR Q+FR+LL+IIYR +ALV PIL
Sbjct: 1 MDISNDASVGSFSIGPSTIVGRTVAFRILFCKSVSQLRHQIFRVLLHIIYRLKALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SWMHPRNPQGILAMVTIIAFLLKRYT+VKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWMHPRNPQGILAMVTIIAFLLKRYTSVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKETP+MNESNLYD ELVRNKLQELR RRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDVELVRNKLQELRRRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKL+FMHETRH FGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLAFMHETRHTFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVE+KLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVENKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGE+VPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGELVPYHPPFNLDPEEGSGTSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPM+QLKELFNVNHFIVSQANPHIAPLLRMKEF RA+GGNFAAKLAHLVEM
Sbjct: 421 WRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRMKEFIRAYGGNFAAKLAHLVEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGFRLGGIA+LFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFRLGGIARLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA----SHSPSIPV 600
GRRCTWEKLSAIKANCGIELALDECV ILNHMRRLKRSAERAAAAAAA SH PSIPV
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVTILNHMRRLKRSAERAAAAAAATGTTSHGPSIPV 600
Query: 601 KFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFD 660
KFSASRRIPSWNCIARENSTGSLEEEYL D +THHQG+GGSIGAGSS RM RTHR+MF+
Sbjct: 601 KFSASRRIPSWNCIARENSTGSLEEEYLTDTPTTHHQGVGGSIGAGSSSRMLRTHRSMFE 660
Query: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQ 720
GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLN+GLVANS+ Q +GGSQ
Sbjct: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNKGLVANSNVFQLIGGSQ 720
Query: 721 NSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTL 780
NSQSPRTMSERSS+SPDFD R+LSNRVSSSILL EGDFLQPER PNGIVFN+VKKE+LTL
Sbjct: 721 NSQSPRTMSERSSESPDFDTRELSNRVSSSILLNEGDFLQPERKPNGIVFNIVKKEDLTL 780
Query: 781 ASRSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVN 840
+RSHDSETQNSEVECLQIDCS+K IDASS SD E TP C +E SP+++ N
Sbjct: 781 TNRSHDSETQNSEVECLQIDCSDKYIDASSASDFDDGNEDVTPKACLKE--SSPENDLAN 840
Query: 841 NSEQ-QDGRDQVFMDT 852
+SEQ DGRDQV MD+
Sbjct: 841 HSEQLHDGRDQVSMDS 854
BLAST of MS016963 vs. ExPASy TrEMBL
Match:
A0A6J1EY02 (triacylglycerol lipase SDP1-like OS=Cucurbita moschata OX=3662 GN=LOC111439408 PE=4 SV=1)
HSP 1 Score: 1531.2 bits (3963), Expect = 0.0e+00
Identity = 775/857 (90.43%), Postives = 813/857 (94.87%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISN+ASVGSFSIGPSTIVGRT+AFRILFCKSVSQLR Q+FR+LL+IIYR +ALV PIL
Sbjct: 1 MDISNDASVGSFSIGPSTIVGRTVAFRILFCKSVSQLRHQIFRVLLHIIYRLKALVAPIL 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
LDKET +MNESNLYD ELVRNKLQELR RRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETLKMNESNLYDVELVRNKLQELRRRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQIP+LIKEYINEVSTQLRLVCDSDSEEL LEEKL+FMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPRLIKEYINEVSTQLRLVCDSDSEELFLEEKLAFMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVVKTLVE+KLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVENKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGE+VPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGELVPYHPPFNLDPEEGSGSSARR 420
Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
WRDGSLEIDLPM+QLKELFNVNHFIVSQANPHIAPLLRMKEF RA+GGNFAAKLAHLVEM
Sbjct: 421 WRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRMKEFIRAYGGNFAAKLAHLVEM 480
Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
EVKHRCNQ+LELGFRLGGIA+LFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFRLGGIARLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA----SHSPSIPV 600
GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA SH PSIPV
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATGTTSHGPSIPV 600
Query: 601 KFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFD 660
KFSASRRIPSWNCIARENSTGSLEEEYL D ++THHQG+GGSIGAGSS RM RTHR+MF+
Sbjct: 601 KFSASRRIPSWNCIARENSTGSLEEEYLTDTSTTHHQGVGGSIGAGSSSRMLRTHRSMFE 660
Query: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQ 720
GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLN+GLVANS+ Q VG SQ
Sbjct: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNKGLVANSNVFQPVGSSQ 720
Query: 721 NSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTL 780
NSQSPRTMSERSS+SPD D R+LSNRVSSSILL EGDFLQPERIPNGIVFN+VKKE+LTL
Sbjct: 721 NSQSPRTMSERSSESPDVDARELSNRVSSSILLNEGDFLQPERIPNGIVFNIVKKEDLTL 780
Query: 781 ASRSHDSETQNSEVECLQIDCSEKDIDASSVSDC-GGNEEYATPHGCFQEPIPSPKSNPV 840
+RSHDSETQNSEVECLQIDCS+K IDASS SD GNEE TP C QE +P+++
Sbjct: 781 TNRSHDSETQNSEVECLQIDCSDKYIDASSASDFDDGNEEDVTPKACLQE--SAPENDLA 840
Query: 841 NNSEQ-QDGRDQVFMDT 852
N+SEQ DG+DQV MD+
Sbjct: 841 NHSEQLHDGQDQVSMDS 855
BLAST of MS016963 vs. TAIR 10
Match:
AT5G04040.1 (Patatin-like phospholipase family protein )
HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 605/823 (73.51%), Postives = 699/823 (84.93%), Query Frame = 0
Query: 1 MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
MDISNEASV FSIGPS+I+GRTIAFR+LFC+S+SQLR+ +FR LL+ RF+ V P +
Sbjct: 1 MDISNEASVDPFSIGPSSIMGRTIAFRVLFCRSMSQLRRDLFRFLLHWFLRFKLTVSPFV 60
Query: 61 SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
SW HPRNPQGILA+VTIIAF+LKRYTNVK +AE+AYRRKFWRNMMR+ALTYEEWAHAAKM
Sbjct: 61 SWFHPRNPQGILAVVTIIAFVLKRYTNVKIKAEMAYRRKFWRNMMRTALTYEEWAHAAKM 120
Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
L+KETP+MNES+LYDEELV+NKLQELRHRRQEGSLRDI+F MRADL RNLGNMCN ELHK
Sbjct: 121 LEKETPKMNESDLYDEELVKNKLQELRHRRQEGSLRDIMFCMRADLVRNLGNMCNSELHK 180
Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
GRLQ+P+ IKEYI+EVSTQLR+VC+SDSEEL LEEKLSFMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQVPRHIKEYIDEVSTQLRMVCNSDSEELSLEEKLSFMHETRHAFGRTALLLSGGASL 240
Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
GAFH GVV+TLVEHKLLPRIIAGSSVGSI+CAVVA+R+WPELQSFF++S HSLQFFDQ+G
Sbjct: 241 GAFHVGVVRTLVEHKLLPRIIAGSSVGSIICAVVASRSWPELQSFFENSLHSLQFFDQLG 300
Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
G+F++V+RVM QGA+H+IRQLQ MLR LTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GVFSIVKRVMTQGALHDIRQLQCMLRNLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGS-GSSAR 420
LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+RSGEIVPYHPPFNLDPE G+ SS R
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRSGEIVPYHPPFNLDPEVGTKSSSGR 420
Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
RWRDGSLE+DLPM+QLKELFNVNHFIVSQANPHIAPLLR+K+ RA+GG FAAKLAHLVE
Sbjct: 421 RWRDGSLEVDLPMMQLKELFNVNHFIVSQANPHIAPLLRLKDLVRAYGGRFAAKLAHLVE 480
Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
MEVKHRCNQ+LELGF LGG+AKLFAQ+WEGDVTVVMPATLAQYSKIIQNPTH++LQK+AN
Sbjct: 481 MEVKHRCNQVLELGFPLGGLAKLFAQEWEGDVTVVMPATLAQYSKIIQNPTHVELQKAAN 540
Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAAS-HSPSIPVKF 600
QGRRCTWEKLSAIK+NCGIELALD+ VAILNHMRRLK+SAERAA A ++S H + +F
Sbjct: 541 QGRRCTWEKLSAIKSNCGIELALDDSVAILNHMRRLKKSAERAATATSSSHHGLASTTRF 600
Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
+ASRRIPSWN +ARENSTGSL++ L + H SSGR S
Sbjct: 601 NASRRIPSWNVLARENSTGSLDD--LVTDNNLH----------ASSGRNL---------S 660
Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLD-DLNRGLVANSSAHQSVGGSQN 720
DSE+E+++L++WTR+GGPLMRTASANKFIDFVQ+LD+D L RG ++ ++ G
Sbjct: 661 DSETESVELSSWTRTGGPLMRTASANKFIDFVQSLDIDIALVRGFSSSPNSPAVPPGGSF 720
Query: 721 SQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLA 780
+ SPR+++ S + + +L +SSI +TEGD LQPER NG V NVVK+ENL +
Sbjct: 721 TPSPRSIAAHSDIESNSNSNNLGTS-TSSITVTEGDLLQPERTSNGFVLNVVKRENLGMP 780
Query: 781 SRSHDSETQNSEV-ECLQIDCSEKDIDASSVS----DCGGNEE 816
S + QN+E+ E +Q+D EK++D SSVS D NEE
Sbjct: 781 SIGN----QNTELPESVQLDIPEKEMDCSSVSEHEEDDNDNEE 797
BLAST of MS016963 vs. TAIR 10
Match:
AT3G57140.1 (sugar-dependent 1-like )
HSP 1 Score: 1071.2 bits (2769), Expect = 4.3e-313
Identity = 565/824 (68.57%), Postives = 656/824 (79.61%), Query Frame = 0
Query: 1 MDISNEASVGSFS-IGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPI 60
MDISNEA V +FS IGP+TI+GRTIA RILFC SVS R +VFRIL + R L+ P
Sbjct: 1 MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPF 60
Query: 61 LSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAK 120
+S +HPRNPQGIL MVT +AFLL RYT++K +AE+AYRRKFWRNMMR+ALTYEEW+HAAK
Sbjct: 61 VSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAK 120
Query: 121 MLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELH 180
MLDKETP++NE++L+D ELV NKL EL+HRR EGSLRDIIF MRADL RNLGNMCNPELH
Sbjct: 121 MLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELH 180
Query: 181 KGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGAS 240
KGRL +P+LIKEYI+EVSTQLR+VCD D+EEL LEEKLSFMHETRHA+GRTALLLSGGAS
Sbjct: 181 KGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGAS 240
Query: 241 LGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQM 300
LGAFH GVVKTLVEHKLLPRIIAGSSVGS+MCAVV TR+WPELQSFF+ SWH+LQFFDQM
Sbjct: 241 LGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQM 300
Query: 301 GGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPR 360
GGIFT V+RVM QGAVHEIR LQ LR LT+NLTFQEAYD+TGRILGITVCS RKHEPPR
Sbjct: 301 GGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPR 360
Query: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSAR 420
CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+R+GEIVPYHPPFNLDPEEGS +S R
Sbjct: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGS-ASVR 420
Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
RWRDGSLE+DLPMIQLKELFNVNHFIVSQANPHIAP LRMKEF RA GG FAAKLA L E
Sbjct: 421 RWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAE 480
Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
MEVKHRCNQ+LELG L +A LFAQ+WEGDVT+VMPAT +QY KIIQNP+++++QK+AN
Sbjct: 481 MEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAAN 540
Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSP------S 600
QGRRCTWEKL+ IKAN GIELALDECV +LNHMRRLKRSAERAAA +A S SP +
Sbjct: 541 QGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLA 600
Query: 601 IPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRN 660
+F+AS+RIPSWNCIAR+NS+GS++++ LA+ AS +Q I +G+G + +
Sbjct: 601 GTNRFNASKRIPSWNCIARQNSSGSVDDDVLAE-ASRLYQHI--VVGSGRNSNRTSNLSH 660
Query: 661 MFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVG 720
+D + SE ++ + WTRSGGPLMRT SA F D+VQNLD D + +S + S+
Sbjct: 661 TYD-AGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQ---IRASENDSIV 720
Query: 721 GSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEN 780
+ +S S SI +TEGD+LQ R NG V N+V+ EN
Sbjct: 721 AASSS-------------------------SHSITVTEGDYLQTGRTHNGFVLNLVRGEN 780
Query: 781 LTLASRSHDSETQNSEV----ECLQIDCSEKDI--DASSVSDCG 812
L + S DS+ + SE+ E +Q+D EKDI SS S+ G
Sbjct: 781 LRMNSEPEDSQNE-SEIPETPESVQLDSPEKDIIDGESSASEDG 790
BLAST of MS016963 vs. TAIR 10
Match:
AT3G57140.2 (sugar-dependent 1-like )
HSP 1 Score: 1071.2 bits (2769), Expect = 4.3e-313
Identity = 565/824 (68.57%), Postives = 656/824 (79.61%), Query Frame = 0
Query: 1 MDISNEASVGSFS-IGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPI 60
MDISNEA V +FS IGP+TI+GRTIA RILFC SVS R +VFRIL + R L+ P
Sbjct: 1 MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPF 60
Query: 61 LSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAK 120
+S +HPRNPQGIL MVT +AFLL RYT++K +AE+AYRRKFWRNMMR+ALTYEEW+HAAK
Sbjct: 61 VSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAK 120
Query: 121 MLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELH 180
MLDKETP++NE++L+D ELV NKL EL+HRR EGSLRDIIF MRADL RNLGNMCNPELH
Sbjct: 121 MLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELH 180
Query: 181 KGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGAS 240
KGRL +P+LIKEYI+EVSTQLR+VCD D+EEL LEEKLSFMHETRHA+GRTALLLSGGAS
Sbjct: 181 KGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGAS 240
Query: 241 LGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQM 300
LGAFH GVVKTLVEHKLLPRIIAGSSVGS+MCAVV TR+WPELQSFF+ SWH+LQFFDQM
Sbjct: 241 LGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQM 300
Query: 301 GGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPR 360
GGIFT V+RVM QGAVHEIR LQ LR LT+NLTFQEAYD+TGRILGITVCS RKHEPPR
Sbjct: 301 GGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPR 360
Query: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSAR 420
CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+R+GEIVPYHPPFNLDPEEGS +S R
Sbjct: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGS-ASVR 420
Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
RWRDGSLE+DLPMIQLKELFNVNHFIVSQANPHIAP LRMKEF RA GG FAAKLA L E
Sbjct: 421 RWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAE 480
Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
MEVKHRCNQ+LELG L +A LFAQ+WEGDVT+VMPAT +QY KIIQNP+++++QK+AN
Sbjct: 481 MEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAAN 540
Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSP------S 600
QGRRCTWEKL+ IKAN GIELALDECV +LNHMRRLKRSAERAAA +A S SP +
Sbjct: 541 QGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLA 600
Query: 601 IPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRN 660
+F+AS+RIPSWNCIAR+NS+GS++++ LA+ AS +Q I +G+G + +
Sbjct: 601 GTNRFNASKRIPSWNCIARQNSSGSVDDDVLAE-ASRLYQHI--VVGSGRNSNRTSNLSH 660
Query: 661 MFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVG 720
+D + SE ++ + WTRSGGPLMRT SA F D+VQNLD D + +S + S+
Sbjct: 661 TYD-AGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQ---IRASENDSIV 720
Query: 721 GSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEN 780
+ +S S SI +TEGD+LQ R NG V N+V+ EN
Sbjct: 721 AASSS-------------------------SHSITVTEGDYLQTGRTHNGFVLNLVRGEN 780
Query: 781 LTLASRSHDSETQNSEV----ECLQIDCSEKDI--DASSVSDCG 812
L + S DS+ + SE+ E +Q+D EKDI SS S+ G
Sbjct: 781 LRMNSEPEDSQNE-SEIPETPESVQLDSPEKDIIDGESSASEDG 790
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022153905.1 | 0.0e+00 | 99.29 | triacylglycerol lipase SDP1 [Momordica charantia] | [more] |
XP_038881740.1 | 0.0e+00 | 92.39 | triacylglycerol lipase SDP1 [Benincasa hispida] | [more] |
XP_008457586.1 | 0.0e+00 | 92.04 | PREDICTED: triacylglycerol lipase SDP1 [Cucumis melo] | [more] |
XP_004142709.1 | 0.0e+00 | 91.45 | triacylglycerol lipase SDP1 [Cucumis sativus] >KGN54525.1 hypothetical protein C... | [more] |
KAG6603329.1 | 0.0e+00 | 91.02 | Triacylglycerol lipase SDP1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
Match Name | E-value | Identity | Description | |
Q9LZA6 | 0.0e+00 | 73.51 | Triacylglycerol lipase SDP1 OS=Arabidopsis thaliana OX=3702 GN=SDP1 PE=1 SV=1 | [more] |
Q9M1I6 | 6.0e-312 | 68.57 | Triacylglycerol lipase SDP1L OS=Arabidopsis thaliana OX=3702 GN=SDP1L PE=1 SV=1 | [more] |
P36165 | 1.1e-65 | 33.54 | Triacylglycerol lipase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... | [more] |
Q12043 | 3.8e-61 | 31.99 | Triacylglycerol lipase 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... | [more] |
Q9Y827 | 1.9e-60 | 33.13 | Triacylglycerol lipase ptl3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DI61 | 0.0e+00 | 99.29 | triacylglycerol lipase SDP1 OS=Momordica charantia OX=3673 GN=LOC111021312 PE=4 ... | [more] |
A0A1S3C753 | 0.0e+00 | 92.04 | triacylglycerol lipase SDP1 OS=Cucumis melo OX=3656 GN=LOC103497252 PE=4 SV=1 | [more] |
A0A0A0KY40 | 0.0e+00 | 91.45 | PNPLA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G358630 PE=4 ... | [more] |
A0A6J1HVX3 | 0.0e+00 | 90.19 | triacylglycerol lipase SDP1-like OS=Cucurbita maxima OX=3661 GN=LOC111467158 PE=... | [more] |
A0A6J1EY02 | 0.0e+00 | 90.43 | triacylglycerol lipase SDP1-like OS=Cucurbita moschata OX=3662 GN=LOC111439408 P... | [more] |