MS016963 (gene) Bitter gourd (TR) v1

Overview
NameMS016963
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontriacylglycerol lipase SDP1
Locationscaffold197: 15059 .. 18247 (-)
RNA-Seq ExpressionMS016963
SyntenyMS016963
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACATTAGTAATGAGGCCAGTGTTGGTTCCTTTTCTATTGGGCCATCGACCATTGTTGGTCGAACAATTGCTTTTAGAATATTGTTTTGCAAATCAGTGTCCCAATTGAGGCAACAAGTGTTCCGTATTTTGCTAAATATCATCTATAGATTTAGGGCCCTTGTACCACCCATACTATCATGGATGCATCCTAGGAATCCACAAGGGATATTGGCAATGGTGACGATTATTGCCTTTTTGTTGAAACGATACACTAATGTCAAAGAGAGGGCCGAATTGGCTTACCGGAGGAAATTTTGGAGAAACATGATGAGATCTGCCTTGACCTATGAGGAGTGGGCTCATGCTGCTAAAATGCTTGACAAAGAGACCCCAAGAATGAACGAGTCGAACCTCTATGACGAAGAGTTGGTGAGGAACAAGCTTCAGGAGCTTCGCCACCGTCGCCAAGAGGGATCTCTCAGAGATATTATATTTTGGATGAGAGCTGATCTCTTCCGAAATCTTGGCAATATGTGCAACCCCGAGCTACACAAGGGTAGGCTTCAAATTCCCAAACTCATAAAGGAGTACATAAATGAGGTTTCAACTCAGTTGAGGTTGGTCTGTGACTCGGACTCGGAGGAACTGTTATTGGAAGAGAAGCTCTCGTTCATGCATGAAACGAGGCATGCATTTGGAAGGACTGCGCTGCTCTTGAGCGGGGGTGCTTCGCTTGGAGCATTTCATGCAGGAGTGGTTAAAACTCTGGTAGAACATAAACTTTTGCCCAGAATAATTGCTGGTTCTAGTGTAGGATCCATCATGTGTGCTGTTGTTGCCACCAGGGCTTGGCCGGAACTACAAAGCTTTTTCGATGATTCTTGGCATTCATTACAGTTTTTCGACCAGATGGGTGGCATTTTTACTGTGGTAAGGAGGGTAATGATACAAGGTGCTGTTCACGAGATTCGGCAATTACAAATTATGTTAAGGCAGCTCACTAGCAACCTAACATTTCAGGAAGCTTATGACATGACAGGTAGAATCCTTGGGATAACAGTTTGTTCCCCGAGGAAGCACGAGCCGCCTAGATGTCTTAACTACTTAACATCTCCTCATGTTGTGATATGGAGTGCTGTAACGGCATCTTGTGCATTCCCTGGCCTTTTCGAGGCACAAGAACTAATGGCCAAGAACAGAAGCGGAGAGATTGTTCCCTATCATCCACCGTTTAATCTGGATCCTGAGGAGGGCTCAGGCTCATCGGCACGTCGTTGGAGAGATGGCAGCTTGGAGATTGATTTGCCGATGATTCAGTTGAAAGAATTGTTCAATGTTAACCATTTCATTGTCAGTCAGGCAAATCCCCATATTGCACCATTGTTGCGAATGAAAGAATTCTTCAGAGCTTTTGGGGGAAATTTTGCTGCCAAGGTATGACAACGTGTGCTCAGGATATTGTTTTGTATCTTTTCTGACTACTGTTCTTTTTTCCATGCGTTGTATGTCCGAAACAAACCACTATACTAATCTTTTTGACAAAACTATAACTTACAGAATGAAATGCTTGTGTTGGAGAAGGGATTGTTTGTTTTCTGATTCTAATTAACTTCTGTGCACATTTAGTTAAAGATATCCTTGGTTTTCCTCCTTTTTATGAGTATTCAAATGTTTCCATATATATCAGAATACATTGGTTACTAAATTAAATTTTTTCATTGACTGCTATGGATATTATCCTTTTTTTTCTCAAAATAACTTCATTTTTGTGCACTCTAGAAACACATATTTCTCATACTCTCTCTCTCTCTCTCTCTCTCTCTCTTACTATTCATCAGTTTTATGGATAGTTGGATAAGTATGAGTATTATTTATCCAGTCTGTCATTTGTAATTTCTCATGGTTGACGTTTGGCTTTGTAGCTTGCTCATCTCGTTGAAATGGAGGTAAAGCATAGATGCAACCAGATTTTAGAACTCGGTTTCCGGTTGGGAGGGATTGCGAAACTTTTTGCTCAAGATTGGGAAGGAGACGTCACAGTCGTCATGCCTGCCACTCTTGCTCAGGTATTTTCTCGTTACAAGACACTACAAATTTCAATGGTTCATAAGAATGGTCAAATGGTACAATGTTAGATTAAAACAAATAAAGCATAGCCAAGTTTTAAAACAATAACTGCGTGTCGATGCTAAAGAACTTTTTTTTTGGTTACAGTACTCGAAAATTATACAAAATCCTACACATTTGGATCTTCAGAAGTCAGCGAACCAAGGTAGGAGGTGCACCTGGGAGAAGCTATCAGCAATAAAAGCCAACTGTGGCATTGAGCTCGCATTGGATGAATGTGTTGCAATTCTCAACCATATGCGGAGGCTGAAACGGAGCGCTGAAAGGGCTGCTGCTGCCGCTGCCGCCTCACACAGCCCTTCGATCCCAGTCAAGTTCAGTGCTTCAAGAAGAATTCCCTCTTGGAACTGCATTGCACGAGAAAACTCAACTGGTTCGCTCGAAGAGGAATACCTCGCCGATGTTGCTTCAACGCACCATCAAGGTATTGGTGGATCGATAGGTGCAGGATCCTCTGGCAGGATGTTTCGAACCCACCGTAACATGTTTGACGGTAGTGATAGCGAATCTGAGAATATCGATTTGAATACTTGGACTAGATCTGGTGGGCCATTGATGAGAACAGCATCAGCTAATAAGTTCATAGACTTTGTTCAAAATTTGGATCTTGATGATCTCAACAGAGGTTTGGTAGCTAATTCCAGTGCACATCAGTCAGTTGGTGGTAGTCAAAATTCTCAAAGCCCCCGAACCATGTCCGAGCGCAGTTCAGACAGCCCTGATTTCGACATGAGGGATCTTAGTAATCGAGTTTCTTCAAGCATTCTTTTAACTGAAGGAGACTTTTTGCAGCCTGAAAGAATACCAAACGGGATTGTGTTCAACGTTGTAAAGAAGGAAAATCTGACTTTGGCGAGTAGGAGCCATGATTCAGAAACTCAAAACAGCGAAGTCGAATGCTTGCAAATCGACTGTTCGGAAAAAGATATCGATGCTAGCTCGGTATCTGATTGTGGTGGCAATGAAGAATATGCCACACCACACGGCTGCTTTCAGGAACCAATACCATCTCCAAAAAGTAATCCAGTCAATAATTCCGAGCAGCAAGATGGTCGAGATCAAGTATTTATGGATACT

mRNA sequence

ATGGACATTAGTAATGAGGCCAGTGTTGGTTCCTTTTCTATTGGGCCATCGACCATTGTTGGTCGAACAATTGCTTTTAGAATATTGTTTTGCAAATCAGTGTCCCAATTGAGGCAACAAGTGTTCCGTATTTTGCTAAATATCATCTATAGATTTAGGGCCCTTGTACCACCCATACTATCATGGATGCATCCTAGGAATCCACAAGGGATATTGGCAATGGTGACGATTATTGCCTTTTTGTTGAAACGATACACTAATGTCAAAGAGAGGGCCGAATTGGCTTACCGGAGGAAATTTTGGAGAAACATGATGAGATCTGCCTTGACCTATGAGGAGTGGGCTCATGCTGCTAAAATGCTTGACAAAGAGACCCCAAGAATGAACGAGTCGAACCTCTATGACGAAGAGTTGGTGAGGAACAAGCTTCAGGAGCTTCGCCACCGTCGCCAAGAGGGATCTCTCAGAGATATTATATTTTGGATGAGAGCTGATCTCTTCCGAAATCTTGGCAATATGTGCAACCCCGAGCTACACAAGGGTAGGCTTCAAATTCCCAAACTCATAAAGGAGTACATAAATGAGGTTTCAACTCAGTTGAGGTTGGTCTGTGACTCGGACTCGGAGGAACTGTTATTGGAAGAGAAGCTCTCGTTCATGCATGAAACGAGGCATGCATTTGGAAGGACTGCGCTGCTCTTGAGCGGGGGTGCTTCGCTTGGAGCATTTCATGCAGGAGTGGTTAAAACTCTGGTAGAACATAAACTTTTGCCCAGAATAATTGCTGGTTCTAGTGTAGGATCCATCATGTGTGCTGTTGTTGCCACCAGGGCTTGGCCGGAACTACAAAGCTTTTTCGATGATTCTTGGCATTCATTACAGTTTTTCGACCAGATGGGTGGCATTTTTACTGTGGTAAGGAGGGTAATGATACAAGGTGCTGTTCACGAGATTCGGCAATTACAAATTATGTTAAGGCAGCTCACTAGCAACCTAACATTTCAGGAAGCTTATGACATGACAGGTAGAATCCTTGGGATAACAGTTTGTTCCCCGAGGAAGCACGAGCCGCCTAGATGTCTTAACTACTTAACATCTCCTCATGTTGTGATATGGAGTGCTGTAACGGCATCTTGTGCATTCCCTGGCCTTTTCGAGGCACAAGAACTAATGGCCAAGAACAGAAGCGGAGAGATTGTTCCCTATCATCCACCGTTTAATCTGGATCCTGAGGAGGGCTCAGGCTCATCGGCACGTCGTTGGAGAGATGGCAGCTTGGAGATTGATTTGCCGATGATTCAGTTGAAAGAATTGTTCAATGTTAACCATTTCATTGTCAGTCAGGCAAATCCCCATATTGCACCATTGTTGCGAATGAAAGAATTCTTCAGAGCTTTTGGGGGAAATTTTGCTGCCAAGCTTGCTCATCTCGTTGAAATGGAGGTAAAGCATAGATGCAACCAGATTTTAGAACTCGGTTTCCGGTTGGGAGGGATTGCGAAACTTTTTGCTCAAGATTGGGAAGGAGACGTCACAGTCGTCATGCCTGCCACTCTTGCTCAGTACTCGAAAATTATACAAAATCCTACACATTTGGATCTTCAGAAGTCAGCGAACCAAGGTAGGAGGTGCACCTGGGAGAAGCTATCAGCAATAAAAGCCAACTGTGGCATTGAGCTCGCATTGGATGAATGTGTTGCAATTCTCAACCATATGCGGAGGCTGAAACGGAGCGCTGAAAGGGCTGCTGCTGCCGCTGCCGCCTCACACAGCCCTTCGATCCCAGTCAAGTTCAGTGCTTCAAGAAGAATTCCCTCTTGGAACTGCATTGCACGAGAAAACTCAACTGGTTCGCTCGAAGAGGAATACCTCGCCGATGTTGCTTCAACGCACCATCAAGGTATTGGTGGATCGATAGGTGCAGGATCCTCTGGCAGGATGTTTCGAACCCACCGTAACATGTTTGACGGTAGTGATAGCGAATCTGAGAATATCGATTTGAATACTTGGACTAGATCTGGTGGGCCATTGATGAGAACAGCATCAGCTAATAAGTTCATAGACTTTGTTCAAAATTTGGATCTTGATGATCTCAACAGAGGTTTGGTAGCTAATTCCAGTGCACATCAGTCAGTTGGTGGTAGTCAAAATTCTCAAAGCCCCCGAACCATGTCCGAGCGCAGTTCAGACAGCCCTGATTTCGACATGAGGGATCTTAGTAATCGAGTTTCTTCAAGCATTCTTTTAACTGAAGGAGACTTTTTGCAGCCTGAAAGAATACCAAACGGGATTGTGTTCAACGTTGTAAAGAAGGAAAATCTGACTTTGGCGAGTAGGAGCCATGATTCAGAAACTCAAAACAGCGAAGTCGAATGCTTGCAAATCGACTGTTCGGAAAAAGATATCGATGCTAGCTCGGTATCTGATTGTGGTGGCAATGAAGAATATGCCACACCACACGGCTGCTTTCAGGAACCAATACCATCTCCAAAAAGTAATCCAGTCAATAATTCCGAGCAGCAAGATGGTCGAGATCAAGTATTTATGGATACT

Coding sequence (CDS)

ATGGACATTAGTAATGAGGCCAGTGTTGGTTCCTTTTCTATTGGGCCATCGACCATTGTTGGTCGAACAATTGCTTTTAGAATATTGTTTTGCAAATCAGTGTCCCAATTGAGGCAACAAGTGTTCCGTATTTTGCTAAATATCATCTATAGATTTAGGGCCCTTGTACCACCCATACTATCATGGATGCATCCTAGGAATCCACAAGGGATATTGGCAATGGTGACGATTATTGCCTTTTTGTTGAAACGATACACTAATGTCAAAGAGAGGGCCGAATTGGCTTACCGGAGGAAATTTTGGAGAAACATGATGAGATCTGCCTTGACCTATGAGGAGTGGGCTCATGCTGCTAAAATGCTTGACAAAGAGACCCCAAGAATGAACGAGTCGAACCTCTATGACGAAGAGTTGGTGAGGAACAAGCTTCAGGAGCTTCGCCACCGTCGCCAAGAGGGATCTCTCAGAGATATTATATTTTGGATGAGAGCTGATCTCTTCCGAAATCTTGGCAATATGTGCAACCCCGAGCTACACAAGGGTAGGCTTCAAATTCCCAAACTCATAAAGGAGTACATAAATGAGGTTTCAACTCAGTTGAGGTTGGTCTGTGACTCGGACTCGGAGGAACTGTTATTGGAAGAGAAGCTCTCGTTCATGCATGAAACGAGGCATGCATTTGGAAGGACTGCGCTGCTCTTGAGCGGGGGTGCTTCGCTTGGAGCATTTCATGCAGGAGTGGTTAAAACTCTGGTAGAACATAAACTTTTGCCCAGAATAATTGCTGGTTCTAGTGTAGGATCCATCATGTGTGCTGTTGTTGCCACCAGGGCTTGGCCGGAACTACAAAGCTTTTTCGATGATTCTTGGCATTCATTACAGTTTTTCGACCAGATGGGTGGCATTTTTACTGTGGTAAGGAGGGTAATGATACAAGGTGCTGTTCACGAGATTCGGCAATTACAAATTATGTTAAGGCAGCTCACTAGCAACCTAACATTTCAGGAAGCTTATGACATGACAGGTAGAATCCTTGGGATAACAGTTTGTTCCCCGAGGAAGCACGAGCCGCCTAGATGTCTTAACTACTTAACATCTCCTCATGTTGTGATATGGAGTGCTGTAACGGCATCTTGTGCATTCCCTGGCCTTTTCGAGGCACAAGAACTAATGGCCAAGAACAGAAGCGGAGAGATTGTTCCCTATCATCCACCGTTTAATCTGGATCCTGAGGAGGGCTCAGGCTCATCGGCACGTCGTTGGAGAGATGGCAGCTTGGAGATTGATTTGCCGATGATTCAGTTGAAAGAATTGTTCAATGTTAACCATTTCATTGTCAGTCAGGCAAATCCCCATATTGCACCATTGTTGCGAATGAAAGAATTCTTCAGAGCTTTTGGGGGAAATTTTGCTGCCAAGCTTGCTCATCTCGTTGAAATGGAGGTAAAGCATAGATGCAACCAGATTTTAGAACTCGGTTTCCGGTTGGGAGGGATTGCGAAACTTTTTGCTCAAGATTGGGAAGGAGACGTCACAGTCGTCATGCCTGCCACTCTTGCTCAGTACTCGAAAATTATACAAAATCCTACACATTTGGATCTTCAGAAGTCAGCGAACCAAGGTAGGAGGTGCACCTGGGAGAAGCTATCAGCAATAAAAGCCAACTGTGGCATTGAGCTCGCATTGGATGAATGTGTTGCAATTCTCAACCATATGCGGAGGCTGAAACGGAGCGCTGAAAGGGCTGCTGCTGCCGCTGCCGCCTCACACAGCCCTTCGATCCCAGTCAAGTTCAGTGCTTCAAGAAGAATTCCCTCTTGGAACTGCATTGCACGAGAAAACTCAACTGGTTCGCTCGAAGAGGAATACCTCGCCGATGTTGCTTCAACGCACCATCAAGGTATTGGTGGATCGATAGGTGCAGGATCCTCTGGCAGGATGTTTCGAACCCACCGTAACATGTTTGACGGTAGTGATAGCGAATCTGAGAATATCGATTTGAATACTTGGACTAGATCTGGTGGGCCATTGATGAGAACAGCATCAGCTAATAAGTTCATAGACTTTGTTCAAAATTTGGATCTTGATGATCTCAACAGAGGTTTGGTAGCTAATTCCAGTGCACATCAGTCAGTTGGTGGTAGTCAAAATTCTCAAAGCCCCCGAACCATGTCCGAGCGCAGTTCAGACAGCCCTGATTTCGACATGAGGGATCTTAGTAATCGAGTTTCTTCAAGCATTCTTTTAACTGAAGGAGACTTTTTGCAGCCTGAAAGAATACCAAACGGGATTGTGTTCAACGTTGTAAAGAAGGAAAATCTGACTTTGGCGAGTAGGAGCCATGATTCAGAAACTCAAAACAGCGAAGTCGAATGCTTGCAAATCGACTGTTCGGAAAAAGATATCGATGCTAGCTCGGTATCTGATTGTGGTGGCAATGAAGAATATGCCACACCACACGGCTGCTTTCAGGAACCAATACCATCTCCAAAAAGTAATCCAGTCAATAATTCCGAGCAGCAAGATGGTCGAGATCAAGTATTTATGGATACT

Protein sequence

MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPILSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKMLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHKGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMGGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEMEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNSEQQDGRDQVFMDT
Homology
BLAST of MS016963 vs. NCBI nr
Match: XP_022153905.1 (triacylglycerol lipase SDP1 [Momordica charantia])

HSP 1 Score: 1678.7 bits (4346), Expect = 0.0e+00
Identity = 845/851 (99.29%), Postives = 847/851 (99.53%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL
Sbjct: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQI KLIKEYINEVSTQLRLVCDSDSEELLLEEKLS MHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQISKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSXMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFFDDSWHSLQFFDQMG
Sbjct: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFDDSWHSLQFFDQMG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLFEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600

Query: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660
           SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS
Sbjct: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660

Query: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720
           ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS
Sbjct: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720

Query: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780
           PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS
Sbjct: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780

Query: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNSEQ 840
           HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPK NPVNNSEQ
Sbjct: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKRNPVNNSEQ 840

Query: 841 QDGRDQVFMDT 852
           QDGRDQVFMDT
Sbjct: 841 QDGRDQVFMDT 851

BLAST of MS016963 vs. NCBI nr
Match: XP_038881740.1 (triacylglycerol lipase SDP1 [Benincasa hispida])

HSP 1 Score: 1558.5 bits (4034), Expect = 0.0e+00
Identity = 789/854 (92.39%), Postives = 816/854 (95.55%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           M+ISNEASVGSFSIGPSTI+GRT+AFRILFCKSV QLR Q+FR+LLNIIYRF+ALV PIL
Sbjct: 1   MEISNEASVGSFSIGPSTIIGRTVAFRILFCKSVLQLRHQLFRVLLNIIYRFKALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNL PEEG G+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLAPEEGPGTSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQILELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQILELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAER--AAAAAAASHSPSIPVKF 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAER  AAAAAAAS+ P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAAAASYGPVIPVKF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           SASRRIPSWNCIARENSTGSLEEEYLAD+ STHHQG+GGS GAGSSGR+ RTHR MFDGS
Sbjct: 601 SASRRIPSWNCIARENSTGSLEEEYLADITSTHHQGVGGSTGAGSSGRLLRTHRGMFDGS 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
           DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDD NRGLVANS+  Q++GGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDFNRGLVANSNVLQTIGGSQNS 720

Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
           QSPRTMSERSS+SP+FD R+LSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPEFDARELSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780

Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
           RS DSETQNSEVECLQIDCSEK IDASS SD  GNEE ATP G  QE  PSPKSNPVN+S
Sbjct: 781 RSQDSETQNSEVECLQIDCSEKYIDASSASDYDGNEEDATPEGVLQE--PSPKSNPVNHS 840

Query: 841 EQQ-DGRDQVFMDT 852
           EQQ DG D + +D+
Sbjct: 841 EQQLDGHDHISIDS 852

BLAST of MS016963 vs. NCBI nr
Match: XP_008457586.1 (PREDICTED: triacylglycerol lipase SDP1 [Cucumis melo])

HSP 1 Score: 1548.9 bits (4009), Expect = 0.0e+00
Identity = 786/854 (92.04%), Postives = 814/854 (95.32%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRFRALV PIL
Sbjct: 1   MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRYQLFRVLLNVIYRFRALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA  ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           SASRRIPSWNCIARENSTGSLEEEYLAD+  TH+QG+GGSIG GSSGRM RTHRNMFDGS
Sbjct: 601 SASRRIPSWNCIARENSTGSLEEEYLADITLTHNQGVGGSIGTGSSGRMLRTHRNMFDGS 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
           DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+  Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVLQTVGGSQNS 720

Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
           QSPRTMSERSS+SPDFD R+LSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDPRELSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780

Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
           RSHDSETQNSEVECLQ+D SE+ +DASS SD  GNEE ATP G  QE +    +N VN S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESLTI--NNLVNRS 840

Query: 841 -EQQDGRDQVFMDT 852
            EQQDG D + MD+
Sbjct: 841 EEQQDGHDHISMDS 852

BLAST of MS016963 vs. NCBI nr
Match: XP_004142709.1 (triacylglycerol lipase SDP1 [Cucumis sativus] >KGN54525.1 hypothetical protein Csa_012774 [Cucumis sativus])

HSP 1 Score: 1543.1 bits (3994), Expect = 0.0e+00
Identity = 781/854 (91.45%), Postives = 815/854 (95.43%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRF+ALV PIL
Sbjct: 1   MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRHQLFRVLLNVIYRFKALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP++NESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKLNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA  ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           SA+RRIPSWNCIARENSTGSLEEEYL D+  TH+QG GGSIGAGSSGRM RTHR++FDGS
Sbjct: 601 SAARRIPSWNCIARENSTGSLEEEYLTDITLTHNQGSGGSIGAGSSGRMLRTHRSIFDGS 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
           DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+  Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVVQTVGGSQNS 720

Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
           QSPRTMSERSS+SPDFD R+LS RVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDARELSTRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780

Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
           RSHDSETQNSEVECLQ+D SE+ +DASS SD  GNEE ATP G  QE +  P +N VN+S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESL--PVNNSVNHS 840

Query: 841 -EQQDGRDQVFMDT 852
            EQQDG D++ MD+
Sbjct: 841 EEQQDGHDRISMDS 852

BLAST of MS016963 vs. NCBI nr
Match: KAG6603329.1 (Triacylglycerol lipase SDP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 780/857 (91.02%), Postives = 815/857 (95.10%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISN+ASVGSFSIGPSTIVGRT+AFRILFCKSVSQLR Q+FR+LL+IIYR +ALV PIL
Sbjct: 1   MDISNDASVGSFSIGPSTIVGRTVAFRILFCKSVSQLRHQIFRVLLHIIYRLKALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYD ELVRNKLQELR RRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDVELVRNKLQELRRRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIP+LIKEYINEVSTQLRLVCDSDSEELLLEEKL+FMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPRLIKEYINEVSTQLRLVCDSDSEELLLEEKLAFMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVE+KLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVENKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGE+VPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGELVPYHPPFNLDPEEGSGSSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPM+QLKELFNVNHFIVSQANPHIAPLLRMKEF RA+GGNFAAKLAHLVEM
Sbjct: 421 WRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRMKEFIRAYGGNFAAKLAHLVEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGFRLGGIA+LFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFRLGGIARLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA----SHSPSIPV 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA    SH PSIPV
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATGTTSHGPSIPV 600

Query: 601 KFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFD 660
           KFSASRRIPSWNCIARENSTGSLEEEYL D  +THHQG+GGSIGAGSS RM RTHR+MF+
Sbjct: 601 KFSASRRIPSWNCIARENSTGSLEEEYLTDTLTTHHQGVGGSIGAGSSSRMLRTHRSMFE 660

Query: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQ 720
           GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLN+GLVANS+  Q VG SQ
Sbjct: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNKGLVANSNVFQPVGSSQ 720

Query: 721 NSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTL 780
           NSQSPRTMSERSS+SPDFD R+LSNRVSSSILL EGDFLQPERIPNGIVFN+VKKE+LTL
Sbjct: 721 NSQSPRTMSERSSESPDFDARELSNRVSSSILLNEGDFLQPERIPNGIVFNIVKKEDLTL 780

Query: 781 ASRSHDSETQNSEVECLQIDCSEKDIDASSVSDC-GGNEEYATPHGCFQEPIPSPKSNPV 840
            +RSHDSETQNSEVECLQIDCS+K IDASS SD   GNEE  TP  C QE   SP+++  
Sbjct: 781 TNRSHDSETQNSEVECLQIDCSDKYIDASSASDFDDGNEEDVTPKACLQE--SSPENDLA 840

Query: 841 NNSEQ-QDGRDQVFMDT 852
           N+SEQ  DGRDQV MD+
Sbjct: 841 NHSEQLHDGRDQVSMDS 855

BLAST of MS016963 vs. ExPASy Swiss-Prot
Match: Q9LZA6 (Triacylglycerol lipase SDP1 OS=Arabidopsis thaliana OX=3702 GN=SDP1 PE=1 SV=1)

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 605/823 (73.51%), Postives = 699/823 (84.93%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISNEASV  FSIGPS+I+GRTIAFR+LFC+S+SQLR+ +FR LL+   RF+  V P +
Sbjct: 1   MDISNEASVDPFSIGPSSIMGRTIAFRVLFCRSMSQLRRDLFRFLLHWFLRFKLTVSPFV 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW HPRNPQGILA+VTIIAF+LKRYTNVK +AE+AYRRKFWRNMMR+ALTYEEWAHAAKM
Sbjct: 61  SWFHPRNPQGILAVVTIIAFVLKRYTNVKIKAEMAYRRKFWRNMMRTALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           L+KETP+MNES+LYDEELV+NKLQELRHRRQEGSLRDI+F MRADL RNLGNMCN ELHK
Sbjct: 121 LEKETPKMNESDLYDEELVKNKLQELRHRRQEGSLRDIMFCMRADLVRNLGNMCNSELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQ+P+ IKEYI+EVSTQLR+VC+SDSEEL LEEKLSFMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQVPRHIKEYIDEVSTQLRMVCNSDSEELSLEEKLSFMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVV+TLVEHKLLPRIIAGSSVGSI+CAVVA+R+WPELQSFF++S HSLQFFDQ+G
Sbjct: 241 GAFHVGVVRTLVEHKLLPRIIAGSSVGSIICAVVASRSWPELQSFFENSLHSLQFFDQLG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           G+F++V+RVM QGA+H+IRQLQ MLR LTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GVFSIVKRVMTQGALHDIRQLQCMLRNLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGS-GSSAR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+RSGEIVPYHPPFNLDPE G+  SS R
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRSGEIVPYHPPFNLDPEVGTKSSSGR 420

Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
           RWRDGSLE+DLPM+QLKELFNVNHFIVSQANPHIAPLLR+K+  RA+GG FAAKLAHLVE
Sbjct: 421 RWRDGSLEVDLPMMQLKELFNVNHFIVSQANPHIAPLLRLKDLVRAYGGRFAAKLAHLVE 480

Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
           MEVKHRCNQ+LELGF LGG+AKLFAQ+WEGDVTVVMPATLAQYSKIIQNPTH++LQK+AN
Sbjct: 481 MEVKHRCNQVLELGFPLGGLAKLFAQEWEGDVTVVMPATLAQYSKIIQNPTHVELQKAAN 540

Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAAS-HSPSIPVKF 600
           QGRRCTWEKLSAIK+NCGIELALD+ VAILNHMRRLK+SAERAA A ++S H  +   +F
Sbjct: 541 QGRRCTWEKLSAIKSNCGIELALDDSVAILNHMRRLKKSAERAATATSSSHHGLASTTRF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           +ASRRIPSWN +ARENSTGSL++  L    + H           SSGR           S
Sbjct: 601 NASRRIPSWNVLARENSTGSLDD--LVTDNNLH----------ASSGRNL---------S 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLD-DLNRGLVANSSAHQSVGGSQN 720
           DSE+E+++L++WTR+GGPLMRTASANKFIDFVQ+LD+D  L RG  ++ ++     G   
Sbjct: 661 DSETESVELSSWTRTGGPLMRTASANKFIDFVQSLDIDIALVRGFSSSPNSPAVPPGGSF 720

Query: 721 SQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLA 780
           + SPR+++  S    + +  +L    +SSI +TEGD LQPER  NG V NVVK+ENL + 
Sbjct: 721 TPSPRSIAAHSDIESNSNSNNLGTS-TSSITVTEGDLLQPERTSNGFVLNVVKRENLGMP 780

Query: 781 SRSHDSETQNSEV-ECLQIDCSEKDIDASSVS----DCGGNEE 816
           S  +    QN+E+ E +Q+D  EK++D SSVS    D   NEE
Sbjct: 781 SIGN----QNTELPESVQLDIPEKEMDCSSVSEHEEDDNDNEE 797

BLAST of MS016963 vs. ExPASy Swiss-Prot
Match: Q9M1I6 (Triacylglycerol lipase SDP1L OS=Arabidopsis thaliana OX=3702 GN=SDP1L PE=1 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 6.0e-312
Identity = 565/824 (68.57%), Postives = 656/824 (79.61%), Query Frame = 0

Query: 1   MDISNEASVGSFS-IGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPI 60
           MDISNEA V +FS IGP+TI+GRTIA RILFC SVS  R +VFRIL   +   R L+ P 
Sbjct: 1   MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPF 60

Query: 61  LSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAK 120
           +S +HPRNPQGIL MVT +AFLL RYT++K +AE+AYRRKFWRNMMR+ALTYEEW+HAAK
Sbjct: 61  VSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAK 120

Query: 121 MLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELH 180
           MLDKETP++NE++L+D ELV NKL EL+HRR EGSLRDIIF MRADL RNLGNMCNPELH
Sbjct: 121 MLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELH 180

Query: 181 KGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGAS 240
           KGRL +P+LIKEYI+EVSTQLR+VCD D+EEL LEEKLSFMHETRHA+GRTALLLSGGAS
Sbjct: 181 KGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGAS 240

Query: 241 LGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQM 300
           LGAFH GVVKTLVEHKLLPRIIAGSSVGS+MCAVV TR+WPELQSFF+ SWH+LQFFDQM
Sbjct: 241 LGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQM 300

Query: 301 GGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPR 360
           GGIFT V+RVM QGAVHEIR LQ  LR LT+NLTFQEAYD+TGRILGITVCS RKHEPPR
Sbjct: 301 GGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPR 360

Query: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSAR 420
           CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+R+GEIVPYHPPFNLDPEEGS +S R
Sbjct: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGS-ASVR 420

Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
           RWRDGSLE+DLPMIQLKELFNVNHFIVSQANPHIAP LRMKEF RA GG FAAKLA L E
Sbjct: 421 RWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAE 480

Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
           MEVKHRCNQ+LELG  L  +A LFAQ+WEGDVT+VMPAT +QY KIIQNP+++++QK+AN
Sbjct: 481 MEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAAN 540

Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSP------S 600
           QGRRCTWEKL+ IKAN GIELALDECV +LNHMRRLKRSAERAAA +A S SP      +
Sbjct: 541 QGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLA 600

Query: 601 IPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRN 660
              +F+AS+RIPSWNCIAR+NS+GS++++ LA+ AS  +Q I   +G+G +        +
Sbjct: 601 GTNRFNASKRIPSWNCIARQNSSGSVDDDVLAE-ASRLYQHI--VVGSGRNSNRTSNLSH 660

Query: 661 MFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVG 720
            +D + SE ++ +   WTRSGGPLMRT SA  F D+VQNLD  D  +     +S + S+ 
Sbjct: 661 TYD-AGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQ---IRASENDSIV 720

Query: 721 GSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEN 780
            + +S                         S SI +TEGD+LQ  R  NG V N+V+ EN
Sbjct: 721 AASSS-------------------------SHSITVTEGDYLQTGRTHNGFVLNLVRGEN 780

Query: 781 LTLASRSHDSETQNSEV----ECLQIDCSEKDI--DASSVSDCG 812
           L + S   DS+ + SE+    E +Q+D  EKDI    SS S+ G
Sbjct: 781 LRMNSEPEDSQNE-SEIPETPESVQLDSPEKDIIDGESSASEDG 790

BLAST of MS016963 vs. ExPASy Swiss-Prot
Match: P36165 (Triacylglycerol lipase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGL4 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 1.1e-65
Identity = 165/492 (33.54%), Postives = 262/492 (53.25%), Query Frame = 0

Query: 108 ALTYEEWAHAAKMLDKETPRMN-----ESNLYDEELVRNKLQELRHRRQEGSLRDIIFWM 167
           A+++EEW  A   LD  T +       ES LYD +L+++    +R  R   +   +++ +
Sbjct: 155 AMSFEEWCSAGARLDDLTGKTEWKQKLESPLYDYKLIKDLTSRMREERLNRNYAQLLYII 214

Query: 168 RADLFRNLGNMCNPELHK-GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMH 227
           R +  RNLGNM N  L++   +    LI EY+ E    L  + +SD ++  L   L  + 
Sbjct: 215 RTNWVRNLGNMGNVNLYRHSHVGTKYLIDEYMMESRLALESLMESDLDDSYL---LGILQ 274

Query: 228 ETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPE 287
           +TR   GRTAL+LSGG + G FH GV+ TL E  LLPR+I+GSS G+I+ ++++     E
Sbjct: 275 QTRRNIGRTALVLSGGGTFGLFHIGVLGTLFELDLLPRVISGSSAGAIVASILSVHHKEE 334

Query: 288 ----LQSFFDDSWHSLQFFDQMG---GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTF 347
               L    D  ++  +   Q      +   + R    G   + + L   + +   +LTF
Sbjct: 335 IPVLLNHILDKEFNIFKDDKQKSESENLLIKISRFFKNGTWFDNKHLVNTMIEFLGDLTF 394

Query: 348 QEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKN 407
           +EAY+ TG+IL ITV      E PR LN LT+P+V+IWSAV ASC+ PG+F +  L  K+
Sbjct: 395 REAYNRTGKILNITVSPASLFEQPRLLNNLTAPNVLIWSAVCASCSLPGIFPSSPLYEKD 454

Query: 408 -RSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHI 467
            ++GE  P+           +GSS+ ++ DGS++ DLP+ +L E+FNV+H I  Q N H+
Sbjct: 455 PKTGERKPW-----------TGSSSVKFVDGSVDNDLPISRLSEMFNVDHIIACQVNIHV 514

Query: 468 APLLRMKEFFRAFGG----NFAAKLAHLVEMEVKHRCNQ---ILELGFRLGGIAK----- 527
            P L++       GG     F+A+L   +        N+   ILE+G  + GIAK     
Sbjct: 515 FPFLKLS--LSCVGGEIEDEFSARLKQNLSSIYNFMANEAIHILEIGSEM-GIAKNALTK 574

Query: 528 ---LFAQDWEGDVTVVMP-ATLAQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAIKANCG 570
              + +Q + GD+T++     L +  +++ NPT   L +    G + TW K+S I+ +CG
Sbjct: 575 LRSVLSQQYSGDITILPDMCMLFRIKELLSNPTKEFLLREITNGAKATWPKVSIIQNHCG 629

BLAST of MS016963 vs. ExPASy Swiss-Prot
Match: Q12043 (Triacylglycerol lipase 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGL5 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.8e-61
Identity = 159/497 (31.99%), Postives = 251/497 (50.50%), Query Frame = 0

Query: 106 RSALTYEEWAHAAKMLD-----KETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIF 165
           + A++Y++W   A  LD      E   ++ES+LY+ +L+++    +RH R       +++
Sbjct: 53  KHAISYDQWNDIASRLDDLTGLSEWKTIDESSLYNYKLLQDLTIRMRHLRTTHDYHRLLY 112

Query: 166 WMRADLFRNLGNMCNPELHK-GRLQIPKLIKEYINEVSTQL-RLVCDSDSEELLLEEKLS 225
            +R    RNLGNM N  L++       ++I +Y+ E    L  L+  S+  +  L   L 
Sbjct: 113 LIRTKWVRNLGNMNNVNLYRHSHTGTKQIIHDYLEESQAVLTALIHQSNMNDHYL---LG 172

Query: 226 FMHETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRA 285
            + +TR   GRTAL+LSGG++ G FH GV+  L E  L+P++I+GSS G+I+ ++     
Sbjct: 173 ILQQTRRNIGRTALVLSGGSTFGLFHIGVLAALFESDLMPKVISGSSAGAIVASIFCVHT 232

Query: 286 WPELQS------------FFDDSWHSLQFFDQMGGIFTVVRRVMIQGAVHEIRQLQIMLR 345
             E+ S            F DD+  S         +   + R    G     + L   + 
Sbjct: 233 TQEIPSLLTNVLNMEFNIFNDDNSKS-----PNENLLIKISRFCQNGTWFNNQPLINTML 292

Query: 346 QLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFE 405
               NLTF+EAY+ TG+IL ITV     +E P+ LN LT+P+V+IWSAV ASC+ PG+F 
Sbjct: 293 SFLGNLTFREAYNKTGKILNITVSPASIYEQPKLLNNLTAPNVLIWSAVCASCSLPGVFP 352

Query: 406 AQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIV 465
           +  L  K+     +      NL        S  ++ DGS++ D+P+ +L E+FNV+H I 
Sbjct: 353 STPLFEKDPHTGKIKEWGATNLH------LSNMKFMDGSVDNDMPISRLSEMFNVDHIIA 412

Query: 466 SQANPHIAPLLRM----------KEFFRAFGGNFAAKLAHLVEMEVKHRCNQILELGFR- 525
            Q N H+ PLL+           KE    F  N   K+      E  H  + + EL F  
Sbjct: 413 CQVNIHVFPLLKFSNTCVGGEIEKEITARF-RNQVTKIFKFFSDETIHFLDILKELEFHP 472

Query: 526 --LGGIAKLFAQDWEGDVTVVMPATL-AQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAI 570
             +  +  LF Q + G+VT++   ++  Q+ ++++NP+ L L      G R TW K+S I
Sbjct: 473 YLMTKLKHLFLQQYSGNVTILPDLSMVGQFHEVLKNPSQLFLLHQTTLGARATWPKISMI 532

BLAST of MS016963 vs. ExPASy Swiss-Prot
Match: Q9Y827 (Triacylglycerol lipase ptl3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ptl3 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.9e-60
Identity = 162/489 (33.13%), Postives = 244/489 (49.90%), Query Frame = 0

Query: 105 MRSALTYEEWAHAAKMLD-----KETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDII 164
           M  A +YE W  AA+ LD      +     ES+ YD  LV ++L ELR  R   + R ++
Sbjct: 10  MEYASSYETWLEAAEKLDVIEGKYQWREQKESDEYDYVLVESRLHELRRHRLSKNTRLLL 69

Query: 165 FWMRADLFRNLGNMCNPELHK-GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLS 224
             +R  + R+  NM N  L+        KLI E+I EV   L  +   ++ +L L+EK++
Sbjct: 70  GLLRNSVARDFANMDNSRLYNYAHSGTKKLIDEFIQEVLMCLTYL--EETPDLSLDEKIT 129

Query: 225 FMHETRHAFGRTALLLSGGASLGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRA 284
                +   G TAL+LSGG + G  H GV+++L E  L+P+II GSS G+I+    A R 
Sbjct: 130 EFSRLKLTTGNTALILSGGGTFGMTHIGVLQSLHEQGLVPKIICGSSAGAIVACAAAVRN 189

Query: 285 WPELQSFFDDSWHS--LQFFDQMG----GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNL 344
             E Q      +H+  L  F         +   V++   +G V +I  L+ +++ L  + 
Sbjct: 190 -KEEQEILLRQFHTGDLSVFTDPNAAPPSVIQSVKQYFTRGCVLDISHLERVMKLLIGDF 249

Query: 345 TFQEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMA 404
           TFQEAYD +G IL +TV      E P  LNY+T+P+V++WSAV A+C+ P LF+   L  
Sbjct: 250 TFQEAYDRSGYILNVTVSCGSLFEMPSLLNYITAPNVLVWSAVVATCSVPFLFKRATLWE 309

Query: 405 KNRSGEIVPYHPPFNLDPEEGSGSSARRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPH 464
           ++          P   +      + A  W DGS++ D+P  +L ELF+VNHFIVSQ N H
Sbjct: 310 RD----------PLTREVSAFCVTDAPLWMDGSVDNDIPHAKLTELFHVNHFIVSQVNFH 369

Query: 465 IAPLLRMKEFFRAFGGNFAAKLAHLVEMEVKHRCNQILELGFRLGGIAKL---FAQDWEG 524
           I P + M      +      K   L   EV        ELG       KL     Q + G
Sbjct: 370 IVPFI-MDPTSHNWVERCCKKAIDLAAQEVSLTFRLFAELGIFSVLFTKLQSVITQKYSG 429

Query: 525 DVTVVMPATLAQYSKIIQNPTHLDLQKSANQGRRCTWEKLSAIKANCGIELALDECVAIL 579
           D+T++      + +K+I+NPT   L  +A +G+R TW K+   + +C IE+ +       
Sbjct: 430 DITIIPRLNYREVNKVIKNPTPSFLLDAATRGKRGTWTKVPVTRNHCAIEILIAAA---- 479

BLAST of MS016963 vs. ExPASy TrEMBL
Match: A0A6J1DI61 (triacylglycerol lipase SDP1 OS=Momordica charantia OX=3673 GN=LOC111021312 PE=4 SV=1)

HSP 1 Score: 1678.7 bits (4346), Expect = 0.0e+00
Identity = 845/851 (99.29%), Postives = 847/851 (99.53%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL
Sbjct: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQI KLIKEYINEVSTQLRLVCDSDSEELLLEEKLS MHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQISKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSXMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFFDDSWHSLQFFDQMG
Sbjct: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFDDSWHSLQFFDQMG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLFEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSPSIPVKFSA 600

Query: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660
           SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS
Sbjct: 601 SRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGSDS 660

Query: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720
           ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS
Sbjct: 661 ESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNSQS 720

Query: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780
           PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS
Sbjct: 721 PRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLASRS 780

Query: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNSEQ 840
           HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPK NPVNNSEQ
Sbjct: 781 HDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKRNPVNNSEQ 840

Query: 841 QDGRDQVFMDT 852
           QDGRDQVFMDT
Sbjct: 841 QDGRDQVFMDT 851

BLAST of MS016963 vs. ExPASy TrEMBL
Match: A0A1S3C753 (triacylglycerol lipase SDP1 OS=Cucumis melo OX=3656 GN=LOC103497252 PE=4 SV=1)

HSP 1 Score: 1548.9 bits (4009), Expect = 0.0e+00
Identity = 786/854 (92.04%), Postives = 814/854 (95.32%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRFRALV PIL
Sbjct: 1   MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRYQLFRVLLNVIYRFRALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA  ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           SASRRIPSWNCIARENSTGSLEEEYLAD+  TH+QG+GGSIG GSSGRM RTHRNMFDGS
Sbjct: 601 SASRRIPSWNCIARENSTGSLEEEYLADITLTHNQGVGGSIGTGSSGRMLRTHRNMFDGS 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
           DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+  Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVLQTVGGSQNS 720

Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
           QSPRTMSERSS+SPDFD R+LSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDPRELSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780

Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
           RSHDSETQNSEVECLQ+D SE+ +DASS SD  GNEE ATP G  QE +    +N VN S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESLTI--NNLVNRS 840

Query: 841 -EQQDGRDQVFMDT 852
            EQQDG D + MD+
Sbjct: 841 EEQQDGHDHISMDS 852

BLAST of MS016963 vs. ExPASy TrEMBL
Match: A0A0A0KY40 (PNPLA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G358630 PE=4 SV=1)

HSP 1 Score: 1543.1 bits (3994), Expect = 0.0e+00
Identity = 781/854 (91.45%), Postives = 815/854 (95.43%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           M+ISNEASVGSFSIGPSTIVGRTIAFRILFCKSV QLR Q+FR+LLN+IYRF+ALV PIL
Sbjct: 1   MEISNEASVGSFSIGPSTIVGRTIAFRILFCKSVLQLRHQLFRVLLNVIYRFKALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW+HPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWIHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP++NESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKLNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSF+HETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFLHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVEHKLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQ+G
Sbjct: 241 GAFHTGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQLG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGTSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEF RA GGNFAAKLAHL EM
Sbjct: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFIRACGGNFAAKLAHLAEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGF LGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFPLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA--ASHSPSIPVKF 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAA  ASH P IPVKF
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATASHGPPIPVKF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           SA+RRIPSWNCIARENSTGSLEEEYL D+  TH+QG GGSIGAGSSGRM RTHR++FDGS
Sbjct: 601 SAARRIPSWNCIARENSTGSLEEEYLTDITLTHNQGSGGSIGAGSSGRMLRTHRSIFDGS 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQNS 720
           DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANS+  Q+VGGSQNS
Sbjct: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSNVVQTVGGSQNS 720

Query: 721 QSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLAS 780
           QSPRTMSERSS+SPDFD R+LS RVSSSILLTEGDFLQPERIPNGIVFNVVKKE+LTL S
Sbjct: 721 QSPRTMSERSSESPDFDARELSTRVSSSILLTEGDFLQPERIPNGIVFNVVKKEDLTLTS 780

Query: 781 RSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVNNS 840
           RSHDSETQNSEVECLQ+D SE+ +DASS SD  GNEE ATP G  QE +  P +N VN+S
Sbjct: 781 RSHDSETQNSEVECLQVDRSEQYVDASSASDYDGNEEDATPKGFLQESL--PVNNSVNHS 840

Query: 841 -EQQDGRDQVFMDT 852
            EQQDG D++ MD+
Sbjct: 841 EEQQDGHDRISMDS 852

BLAST of MS016963 vs. ExPASy TrEMBL
Match: A0A6J1HVX3 (triacylglycerol lipase SDP1-like OS=Cucurbita maxima OX=3661 GN=LOC111467158 PE=4 SV=1)

HSP 1 Score: 1531.2 bits (3963), Expect = 0.0e+00
Identity = 772/856 (90.19%), Postives = 810/856 (94.63%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISN+ASVGSFSIGPSTIVGRT+AFRILFCKSVSQLR Q+FR+LL+IIYR +ALV PIL
Sbjct: 1   MDISNDASVGSFSIGPSTIVGRTVAFRILFCKSVSQLRHQIFRVLLHIIYRLKALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SWMHPRNPQGILAMVTIIAFLLKRYT+VKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWMHPRNPQGILAMVTIIAFLLKRYTSVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKETP+MNESNLYD ELVRNKLQELR RRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETPKMNESNLYDVELVRNKLQELRRRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKL+FMHETRH FGRTALLLSGGASL
Sbjct: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLAFMHETRHTFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVE+KLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVENKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGE+VPYHPPFNLDPEEGSG+SARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGELVPYHPPFNLDPEEGSGTSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPM+QLKELFNVNHFIVSQANPHIAPLLRMKEF RA+GGNFAAKLAHLVEM
Sbjct: 421 WRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRMKEFIRAYGGNFAAKLAHLVEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGFRLGGIA+LFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFRLGGIARLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA----SHSPSIPV 600
           GRRCTWEKLSAIKANCGIELALDECV ILNHMRRLKRSAERAAAAAAA    SH PSIPV
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVTILNHMRRLKRSAERAAAAAAATGTTSHGPSIPV 600

Query: 601 KFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFD 660
           KFSASRRIPSWNCIARENSTGSLEEEYL D  +THHQG+GGSIGAGSS RM RTHR+MF+
Sbjct: 601 KFSASRRIPSWNCIARENSTGSLEEEYLTDTPTTHHQGVGGSIGAGSSSRMLRTHRSMFE 660

Query: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQ 720
           GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLN+GLVANS+  Q +GGSQ
Sbjct: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNKGLVANSNVFQLIGGSQ 720

Query: 721 NSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTL 780
           NSQSPRTMSERSS+SPDFD R+LSNRVSSSILL EGDFLQPER PNGIVFN+VKKE+LTL
Sbjct: 721 NSQSPRTMSERSSESPDFDTRELSNRVSSSILLNEGDFLQPERKPNGIVFNIVKKEDLTL 780

Query: 781 ASRSHDSETQNSEVECLQIDCSEKDIDASSVSDCGGNEEYATPHGCFQEPIPSPKSNPVN 840
            +RSHDSETQNSEVECLQIDCS+K IDASS SD     E  TP  C +E   SP+++  N
Sbjct: 781 TNRSHDSETQNSEVECLQIDCSDKYIDASSASDFDDGNEDVTPKACLKE--SSPENDLAN 840

Query: 841 NSEQ-QDGRDQVFMDT 852
           +SEQ  DGRDQV MD+
Sbjct: 841 HSEQLHDGRDQVSMDS 854

BLAST of MS016963 vs. ExPASy TrEMBL
Match: A0A6J1EY02 (triacylglycerol lipase SDP1-like OS=Cucurbita moschata OX=3662 GN=LOC111439408 PE=4 SV=1)

HSP 1 Score: 1531.2 bits (3963), Expect = 0.0e+00
Identity = 775/857 (90.43%), Postives = 813/857 (94.87%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISN+ASVGSFSIGPSTIVGRT+AFRILFCKSVSQLR Q+FR+LL+IIYR +ALV PIL
Sbjct: 1   MDISNDASVGSFSIGPSTIVGRTVAFRILFCKSVSQLRHQIFRVLLHIIYRLKALVAPIL 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM
Sbjct: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           LDKET +MNESNLYD ELVRNKLQELR RRQEGSLRDIIFWMRADLFRNLGNMCNPELHK
Sbjct: 121 LDKETLKMNESNLYDVELVRNKLQELRRRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQIP+LIKEYINEVSTQLRLVCDSDSEEL LEEKL+FMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQIPRLIKEYINEVSTQLRLVCDSDSEELFLEEKLAFMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVVKTLVE+KLLPRIIAGSSVGSIMCAVVATR+WPELQSFF+DSWHSLQFFDQMG
Sbjct: 241 GAFHTGVVKTLVENKLLPRIIAGSSVGSIMCAVVATRSWPELQSFFEDSWHSLQFFDQMG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           GIFTVVRRVMIQGAVHEIRQLQ+MLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GIFTVVRRVMIQGAVHEIRQLQMMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSARR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGE+VPYHPPFNLDPEEGSGSSARR
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGELVPYHPPFNLDPEEGSGSSARR 420

Query: 421 WRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVEM 480
           WRDGSLEIDLPM+QLKELFNVNHFIVSQANPHIAPLLRMKEF RA+GGNFAAKLAHLVEM
Sbjct: 421 WRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRMKEFIRAYGGNFAAKLAHLVEM 480

Query: 481 EVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540
           EVKHRCNQ+LELGFRLGGIA+LFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ
Sbjct: 481 EVKHRCNQVLELGFRLGGIARLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSANQ 540

Query: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA----SHSPSIPV 600
           GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAA    SH PSIPV
Sbjct: 541 GRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAATGTTSHGPSIPV 600

Query: 601 KFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFD 660
           KFSASRRIPSWNCIARENSTGSLEEEYL D ++THHQG+GGSIGAGSS RM RTHR+MF+
Sbjct: 601 KFSASRRIPSWNCIARENSTGSLEEEYLTDTSTTHHQGVGGSIGAGSSSRMLRTHRSMFE 660

Query: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVGGSQ 720
           GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLN+GLVANS+  Q VG SQ
Sbjct: 661 GSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNKGLVANSNVFQPVGSSQ 720

Query: 721 NSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTL 780
           NSQSPRTMSERSS+SPD D R+LSNRVSSSILL EGDFLQPERIPNGIVFN+VKKE+LTL
Sbjct: 721 NSQSPRTMSERSSESPDVDARELSNRVSSSILLNEGDFLQPERIPNGIVFNIVKKEDLTL 780

Query: 781 ASRSHDSETQNSEVECLQIDCSEKDIDASSVSDC-GGNEEYATPHGCFQEPIPSPKSNPV 840
            +RSHDSETQNSEVECLQIDCS+K IDASS SD   GNEE  TP  C QE   +P+++  
Sbjct: 781 TNRSHDSETQNSEVECLQIDCSDKYIDASSASDFDDGNEEDVTPKACLQE--SAPENDLA 840

Query: 841 NNSEQ-QDGRDQVFMDT 852
           N+SEQ  DG+DQV MD+
Sbjct: 841 NHSEQLHDGQDQVSMDS 855

BLAST of MS016963 vs. TAIR 10
Match: AT5G04040.1 (Patatin-like phospholipase family protein )

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 605/823 (73.51%), Postives = 699/823 (84.93%), Query Frame = 0

Query: 1   MDISNEASVGSFSIGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPIL 60
           MDISNEASV  FSIGPS+I+GRTIAFR+LFC+S+SQLR+ +FR LL+   RF+  V P +
Sbjct: 1   MDISNEASVDPFSIGPSSIMGRTIAFRVLFCRSMSQLRRDLFRFLLHWFLRFKLTVSPFV 60

Query: 61  SWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAKM 120
           SW HPRNPQGILA+VTIIAF+LKRYTNVK +AE+AYRRKFWRNMMR+ALTYEEWAHAAKM
Sbjct: 61  SWFHPRNPQGILAVVTIIAFVLKRYTNVKIKAEMAYRRKFWRNMMRTALTYEEWAHAAKM 120

Query: 121 LDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELHK 180
           L+KETP+MNES+LYDEELV+NKLQELRHRRQEGSLRDI+F MRADL RNLGNMCN ELHK
Sbjct: 121 LEKETPKMNESDLYDEELVKNKLQELRHRRQEGSLRDIMFCMRADLVRNLGNMCNSELHK 180

Query: 181 GRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGASL 240
           GRLQ+P+ IKEYI+EVSTQLR+VC+SDSEEL LEEKLSFMHETRHAFGRTALLLSGGASL
Sbjct: 181 GRLQVPRHIKEYIDEVSTQLRMVCNSDSEELSLEEKLSFMHETRHAFGRTALLLSGGASL 240

Query: 241 GAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQMG 300
           GAFH GVV+TLVEHKLLPRIIAGSSVGSI+CAVVA+R+WPELQSFF++S HSLQFFDQ+G
Sbjct: 241 GAFHVGVVRTLVEHKLLPRIIAGSSVGSIICAVVASRSWPELQSFFENSLHSLQFFDQLG 300

Query: 301 GIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360
           G+F++V+RVM QGA+H+IRQLQ MLR LTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC
Sbjct: 301 GVFSIVKRVMTQGALHDIRQLQCMLRNLTSNLTFQEAYDMTGRILGITVCSPRKHEPPRC 360

Query: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGS-GSSAR 420
           LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+RSGEIVPYHPPFNLDPE G+  SS R
Sbjct: 361 LNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRSGEIVPYHPPFNLDPEVGTKSSSGR 420

Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
           RWRDGSLE+DLPM+QLKELFNVNHFIVSQANPHIAPLLR+K+  RA+GG FAAKLAHLVE
Sbjct: 421 RWRDGSLEVDLPMMQLKELFNVNHFIVSQANPHIAPLLRLKDLVRAYGGRFAAKLAHLVE 480

Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
           MEVKHRCNQ+LELGF LGG+AKLFAQ+WEGDVTVVMPATLAQYSKIIQNPTH++LQK+AN
Sbjct: 481 MEVKHRCNQVLELGFPLGGLAKLFAQEWEGDVTVVMPATLAQYSKIIQNPTHVELQKAAN 540

Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAAS-HSPSIPVKF 600
           QGRRCTWEKLSAIK+NCGIELALD+ VAILNHMRRLK+SAERAA A ++S H  +   +F
Sbjct: 541 QGRRCTWEKLSAIKSNCGIELALDDSVAILNHMRRLKKSAERAATATSSSHHGLASTTRF 600

Query: 601 SASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRNMFDGS 660
           +ASRRIPSWN +ARENSTGSL++  L    + H           SSGR           S
Sbjct: 601 NASRRIPSWNVLARENSTGSLDD--LVTDNNLH----------ASSGRNL---------S 660

Query: 661 DSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLD-DLNRGLVANSSAHQSVGGSQN 720
           DSE+E+++L++WTR+GGPLMRTASANKFIDFVQ+LD+D  L RG  ++ ++     G   
Sbjct: 661 DSETESVELSSWTRTGGPLMRTASANKFIDFVQSLDIDIALVRGFSSSPNSPAVPPGGSF 720

Query: 721 SQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKENLTLA 780
           + SPR+++  S    + +  +L    +SSI +TEGD LQPER  NG V NVVK+ENL + 
Sbjct: 721 TPSPRSIAAHSDIESNSNSNNLGTS-TSSITVTEGDLLQPERTSNGFVLNVVKRENLGMP 780

Query: 781 SRSHDSETQNSEV-ECLQIDCSEKDIDASSVS----DCGGNEE 816
           S  +    QN+E+ E +Q+D  EK++D SSVS    D   NEE
Sbjct: 781 SIGN----QNTELPESVQLDIPEKEMDCSSVSEHEEDDNDNEE 797

BLAST of MS016963 vs. TAIR 10
Match: AT3G57140.1 (sugar-dependent 1-like )

HSP 1 Score: 1071.2 bits (2769), Expect = 4.3e-313
Identity = 565/824 (68.57%), Postives = 656/824 (79.61%), Query Frame = 0

Query: 1   MDISNEASVGSFS-IGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPI 60
           MDISNEA V +FS IGP+TI+GRTIA RILFC SVS  R +VFRIL   +   R L+ P 
Sbjct: 1   MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPF 60

Query: 61  LSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAK 120
           +S +HPRNPQGIL MVT +AFLL RYT++K +AE+AYRRKFWRNMMR+ALTYEEW+HAAK
Sbjct: 61  VSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAK 120

Query: 121 MLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELH 180
           MLDKETP++NE++L+D ELV NKL EL+HRR EGSLRDIIF MRADL RNLGNMCNPELH
Sbjct: 121 MLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELH 180

Query: 181 KGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGAS 240
           KGRL +P+LIKEYI+EVSTQLR+VCD D+EEL LEEKLSFMHETRHA+GRTALLLSGGAS
Sbjct: 181 KGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGAS 240

Query: 241 LGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQM 300
           LGAFH GVVKTLVEHKLLPRIIAGSSVGS+MCAVV TR+WPELQSFF+ SWH+LQFFDQM
Sbjct: 241 LGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQM 300

Query: 301 GGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPR 360
           GGIFT V+RVM QGAVHEIR LQ  LR LT+NLTFQEAYD+TGRILGITVCS RKHEPPR
Sbjct: 301 GGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPR 360

Query: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSAR 420
           CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+R+GEIVPYHPPFNLDPEEGS +S R
Sbjct: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGS-ASVR 420

Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
           RWRDGSLE+DLPMIQLKELFNVNHFIVSQANPHIAP LRMKEF RA GG FAAKLA L E
Sbjct: 421 RWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAE 480

Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
           MEVKHRCNQ+LELG  L  +A LFAQ+WEGDVT+VMPAT +QY KIIQNP+++++QK+AN
Sbjct: 481 MEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAAN 540

Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSP------S 600
           QGRRCTWEKL+ IKAN GIELALDECV +LNHMRRLKRSAERAAA +A S SP      +
Sbjct: 541 QGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLA 600

Query: 601 IPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRN 660
              +F+AS+RIPSWNCIAR+NS+GS++++ LA+ AS  +Q I   +G+G +        +
Sbjct: 601 GTNRFNASKRIPSWNCIARQNSSGSVDDDVLAE-ASRLYQHI--VVGSGRNSNRTSNLSH 660

Query: 661 MFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVG 720
            +D + SE ++ +   WTRSGGPLMRT SA  F D+VQNLD  D  +     +S + S+ 
Sbjct: 661 TYD-AGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQ---IRASENDSIV 720

Query: 721 GSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEN 780
            + +S                         S SI +TEGD+LQ  R  NG V N+V+ EN
Sbjct: 721 AASSS-------------------------SHSITVTEGDYLQTGRTHNGFVLNLVRGEN 780

Query: 781 LTLASRSHDSETQNSEV----ECLQIDCSEKDI--DASSVSDCG 812
           L + S   DS+ + SE+    E +Q+D  EKDI    SS S+ G
Sbjct: 781 LRMNSEPEDSQNE-SEIPETPESVQLDSPEKDIIDGESSASEDG 790

BLAST of MS016963 vs. TAIR 10
Match: AT3G57140.2 (sugar-dependent 1-like )

HSP 1 Score: 1071.2 bits (2769), Expect = 4.3e-313
Identity = 565/824 (68.57%), Postives = 656/824 (79.61%), Query Frame = 0

Query: 1   MDISNEASVGSFS-IGPSTIVGRTIAFRILFCKSVSQLRQQVFRILLNIIYRFRALVPPI 60
           MDISNEA V +FS IGP+TI+GRTIA RILFC SVS  R +VFRIL   +   R L+ P 
Sbjct: 1   MDISNEAGVDAFSIIGPTTIIGRTIAVRILFCNSVSIFRHKVFRILKFFLRGGRVLLSPF 60

Query: 61  LSWMHPRNPQGILAMVTIIAFLLKRYTNVKERAELAYRRKFWRNMMRSALTYEEWAHAAK 120
           +S +HPRNPQGIL MVT +AFLL RYT++K +AE+AYRRKFWRNMMR+ALTYEEW+HAAK
Sbjct: 61  VSLLHPRNPQGILVMVTTMAFLLNRYTSLKAKAEMAYRRKFWRNMMRAALTYEEWSHAAK 120

Query: 121 MLDKETPRMNESNLYDEELVRNKLQELRHRRQEGSLRDIIFWMRADLFRNLGNMCNPELH 180
           MLDKETP++NE++L+D ELV NKL EL+HRR EGSLRDIIF MRADL RNLGNMCNPELH
Sbjct: 121 MLDKETPKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCMRADLVRNLGNMCNPELH 180

Query: 181 KGRLQIPKLIKEYINEVSTQLRLVCDSDSEELLLEEKLSFMHETRHAFGRTALLLSGGAS 240
           KGRL +P+LIKEYI+EVSTQLR+VCD D+EEL LEEKLSFMHETRHA+GRTALLLSGGAS
Sbjct: 181 KGRLHVPRLIKEYIDEVSTQLRMVCDMDTEELSLEEKLSFMHETRHAYGRTALLLSGGAS 240

Query: 241 LGAFHAGVVKTLVEHKLLPRIIAGSSVGSIMCAVVATRAWPELQSFFDDSWHSLQFFDQM 300
           LGAFH GVVKTLVEHKLLPRIIAGSSVGS+MCAVV TR+WPELQSFF+ SWH+LQFFDQM
Sbjct: 241 LGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWPELQSFFEGSWHALQFFDQM 300

Query: 301 GGIFTVVRRVMIQGAVHEIRQLQIMLRQLTSNLTFQEAYDMTGRILGITVCSPRKHEPPR 360
           GGIFT V+RVM QGAVHEIR LQ  LR LT+NLTFQEAYD+TGRILGITVCS RKHEPPR
Sbjct: 301 GGIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLTFQEAYDITGRILGITVCSLRKHEPPR 360

Query: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGEIVPYHPPFNLDPEEGSGSSAR 420
           CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK+R+GEIVPYHPPFNLDPEEGS +S R
Sbjct: 361 CLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRTGEIVPYHPPFNLDPEEGS-ASVR 420

Query: 421 RWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRMKEFFRAFGGNFAAKLAHLVE 480
           RWRDGSLE+DLPMIQLKELFNVNHFIVSQANPHIAP LRMKEF RA GG FAAKLA L E
Sbjct: 421 RWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHIAPFLRMKEFVRACGGRFAAKLAQLAE 480

Query: 481 MEVKHRCNQILELGFRLGGIAKLFAQDWEGDVTVVMPATLAQYSKIIQNPTHLDLQKSAN 540
           MEVKHRCNQ+LELG  L  +A LFAQ+WEGDVT+VMPAT +QY KIIQNP+++++QK+AN
Sbjct: 481 MEVKHRCNQVLELGLPLREVASLFAQEWEGDVTIVMPATFSQYLKIIQNPSNVEIQKAAN 540

Query: 541 QGRRCTWEKLSAIKANCGIELALDECVAILNHMRRLKRSAERAAAAAAASHSP------S 600
           QGRRCTWEKL+ IKAN GIELALDECV +LNHMRRLKRSAERAAA +A S SP      +
Sbjct: 541 QGRRCTWEKLAVIKANFGIELALDECVTVLNHMRRLKRSAERAAAFSAISSSPPSKHLLA 600

Query: 601 IPVKFSASRRIPSWNCIARENSTGSLEEEYLADVASTHHQGIGGSIGAGSSGRMFRTHRN 660
              +F+AS+RIPSWNCIAR+NS+GS++++ LA+ AS  +Q I   +G+G +        +
Sbjct: 601 GTNRFNASKRIPSWNCIARQNSSGSVDDDVLAE-ASRLYQHI--VVGSGRNSNRTSNLSH 660

Query: 661 MFDGSDSESENIDLNTWTRSGGPLMRTASANKFIDFVQNLDLDDLNRGLVANSSAHQSVG 720
            +D + SE ++ +   WTRSGGPLMRT SA  F D+VQNLD  D  +     +S + S+ 
Sbjct: 661 TYD-AGSECDSPEAEDWTRSGGPLMRTNSAQMFTDYVQNLDAVDPEQ---IRASENDSIV 720

Query: 721 GSQNSQSPRTMSERSSDSPDFDMRDLSNRVSSSILLTEGDFLQPERIPNGIVFNVVKKEN 780
            + +S                         S SI +TEGD+LQ  R  NG V N+V+ EN
Sbjct: 721 AASSS-------------------------SHSITVTEGDYLQTGRTHNGFVLNLVRGEN 780

Query: 781 LTLASRSHDSETQNSEV----ECLQIDCSEKDI--DASSVSDCG 812
           L + S   DS+ + SE+    E +Q+D  EKDI    SS S+ G
Sbjct: 781 LRMNSEPEDSQNE-SEIPETPESVQLDSPEKDIIDGESSASEDG 790

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153905.10.0e+0099.29triacylglycerol lipase SDP1 [Momordica charantia][more]
XP_038881740.10.0e+0092.39triacylglycerol lipase SDP1 [Benincasa hispida][more]
XP_008457586.10.0e+0092.04PREDICTED: triacylglycerol lipase SDP1 [Cucumis melo][more]
XP_004142709.10.0e+0091.45triacylglycerol lipase SDP1 [Cucumis sativus] >KGN54525.1 hypothetical protein C... [more]
KAG6603329.10.0e+0091.02Triacylglycerol lipase SDP1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LZA60.0e+0073.51Triacylglycerol lipase SDP1 OS=Arabidopsis thaliana OX=3702 GN=SDP1 PE=1 SV=1[more]
Q9M1I66.0e-31268.57Triacylglycerol lipase SDP1L OS=Arabidopsis thaliana OX=3702 GN=SDP1L PE=1 SV=1[more]
P361651.1e-6533.54Triacylglycerol lipase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... [more]
Q120433.8e-6131.99Triacylglycerol lipase 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c... [more]
Q9Y8271.9e-6033.13Triacylglycerol lipase ptl3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... [more]
Match NameE-valueIdentityDescription
A0A6J1DI610.0e+0099.29triacylglycerol lipase SDP1 OS=Momordica charantia OX=3673 GN=LOC111021312 PE=4 ... [more]
A0A1S3C7530.0e+0092.04triacylglycerol lipase SDP1 OS=Cucumis melo OX=3656 GN=LOC103497252 PE=4 SV=1[more]
A0A0A0KY400.0e+0091.45PNPLA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G358630 PE=4 ... [more]
A0A6J1HVX30.0e+0090.19triacylglycerol lipase SDP1-like OS=Cucurbita maxima OX=3661 GN=LOC111467158 PE=... [more]
A0A6J1EY020.0e+0090.43triacylglycerol lipase SDP1-like OS=Cucurbita moschata OX=3662 GN=LOC111439408 P... [more]
Match NameE-valueIdentityDescription
AT5G04040.10.0e+0073.51Patatin-like phospholipase family protein [more]
AT3G57140.14.3e-31368.57sugar-dependent 1-like [more]
AT3G57140.24.3e-31368.57sugar-dependent 1-like [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 311..331
NoneNo IPR availableGENE3D3.40.1090.10Cytosolic phospholipase A2 catalytic domaincoord: 220..310
e-value: 2.1E-12
score: 49.2
NoneNo IPR availableGENE3D3.40.1090.10Cytosolic phospholipase A2 catalytic domaincoord: 311..493
e-value: 3.3E-9
score: 38.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 804..851
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 706..727
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 829..844
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 706..738
NoneNo IPR availablePANTHERPTHR14226NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTERcoord: 2..721
NoneNo IPR availablePANTHERPTHR14226:SF72TRIACYLGLYCEROL LIPASE-RELATEDcoord: 2..721
NoneNo IPR availableCDDcd07231Pat_SDP1-likecoord: 162..570
e-value: 0.0
score: 580.563
IPR021771Triacylglycerol lipase, N-terminalPFAMPF11815DUF3336coord: 96..225
e-value: 5.0E-28
score: 97.6
IPR002641Patatin-like phospholipase domainPFAMPF01734Patatincoord: 232..424
e-value: 1.8E-15
score: 57.7
IPR002641Patatin-like phospholipase domainPROSITEPS51635PNPLAcoord: 232..436
score: 39.913486
IPR016035Acyl transferase/acyl hydrolase/lysophospholipaseSUPERFAMILY52151FabD/lysophospholipase-likecoord: 228..575

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016963.1MS016963.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004806 triglyceride lipase activity