MS016959 (gene) Bitter gourd (TR) v1

Overview
NameMS016959
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCysteine synthase
Locationscaffold976: 296690 .. 301899 (+)
RNA-Seq ExpressionMS016959
SyntenyMS016959
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCGCTGCTTCGAGAATTTTGCTGAAGCAGAGAGCCGCGATTTGGTCCGGCAGTTCCACGGTGAGGAAATTGTTTTCCACTCAAACGGTTCTCGATTCTCCTTCTTTCGCTCAGCGGCTCAGAGATCTGCCCAAGGATCTTCCTGGAACCAGTATCAGACGAGATGCTGCTCATGTAAATTTCTCAATCCCTTTCCGTTTCATTCTCTGATCCGTTCTTGCTTTTTCCTTTCCAAATTTCTGGTTTCTCTGATGCCTCCCGATGAGCTTCGAAATTACGCTTCCTCAAGTTCTTCTTCTTCCTTCGATTTTGATTTTCTTTTTCTTCGGATTTCTATGGAAGTTTTACCATCGAGCTCACGAGTCACGAGAGAGATTAGTTTTATTATTTTTTTAATGATTTTCTTTTTTTTTTTAATTAATTTGACGGGCTGGTCCAAGGGATATTACTTTTTTATTTTCTGGCCGTTGACACAGTTCTTAATCATGAGAAAACGCCGTTCAAAATGAATGGAAAAAAGAGTTATTATTATTTATGATTATTTTTATTTTTATTTTTTTTACAAATGTACCAAATAGTTCTCTAATACTTTTGGTAATTTCTTAAAAAGAAAAGTTAAAATTTAACAGATGACGTCTGATTTAAAAAAATTGATGTCTGCTTTAACTGTATACATATAAAAAAAGATTTTGTTAACAAATGGGTTGAATTTTTATGCGTATGAGTAGCGTGTAGGTGATACGTTAATGTATCCAAATATGTTAATACATCAAATTTTTTTTTAATCAAAGTTTTTATGGAATGACTTAATTAAAGATTCTAATGATTAATCTTTTTTTCTTTATTAAGGCTAAGATTGAGAATTTTAAAAAATTTTTGATAAAATTGAAAATTACATAAGGTATATATATGTGTGTATTTAAACCATTGTTTATTTAGTCAATCGATAAAACAGCTACTATACTTTACTTGTACGCCTTTAAAATATTAATATTTCTAAATAATTCTGTTATTTATGTTATTTTTAGTTTCTGCATGAATTCGAACCTCTAACATTTTGAATGGATAATATATATCTTAACCAATTGAAATATTGATCAAATTTAGACAAATTATTGAATGATACTTTTATAATGTTGAATGAGATCTTTATAATGTGTTTGACTCCTGATCATTGAATGTAGATCTGTAAATTTCTATACCCCTAATAATCATTAGATATAAATTCGATTTATTTTGTAGTTTCTATCTCACACATCCAAAACTATATAAAACTACCGGCCACGTTGGGACGACCTCTTCCAATAATTTTTCAAAAAAACTATTAAATGATATTCGTAGTAAAACTTTCTGTTCTGTTTCTCAAACAAAAAGGAAAAAGAAAATCTCTCATGTAGATTTATTTTAATATTACGTAGATCTATTTAGTAATTTTAATATATTTTTAACAATATTTTGAGATGGTTAGGGGACATTCAACAAGTAAAAAGAAAAGAAAAATCACAATTTCACAAACCACATCCTGTAATGAAAAAATTTAAAATAAAACTAACCCTGAGGGGAAAAAAGAAATTTCACTCCATATTATATATATGTATTTCACAACTTTTCAATTTTCTAATATAGCATAAGCTTTTTTTAGTGTTTACATTGAGTTTGGGTTCGATTTATATTAATGTTATTTTTGTAAATTTTCGAACTTTCTGCTATATTTGAAAATACATATATATTTTTTTTCTGGCTATTTATGTTAATATGAATCTAATGTTCTTCCAAATCTTCATTTTCATTTTCATTTTTTCAGCTCATTGGCAAAACTCCAATAGTATATCTTAACAAAGTCACTGAAGACTGTGGAGCTTATGTTGCTGTGAAGCAAGAGATGATGCAGCCTACTTCCAGCATTAAAGACAGGTTCCCACTCTATTCATGATTGAGCTAAAGATTTTCTAAGGAAGGAGCAAAAATTAGAGAAAAACTACACAATTTTAAATACTATATCTATACTTTTTGAATTACAACAAATTAAGAGGGTAAATTGCCCTGCCTTTCCTTTGTTGCTCCTGACTCTGTATCTTTGATCACTTTCATTTCATCATCAGACCGGCTTTCGCGATGATCACCGATGCTGAAAAGAAAGGTTTGATTACTCCAGGAAAGGTCAGCTCTATCAGCACTTAGATTGCAACATTTTGCTGTATTTTATACTTGGGATACTTTTCAACAAAACTTGTTTTTTCTGAACTTCACTCTGTTTTACGATGTAGACGACATTGATCGAACCAACGTCGGGAAATATGGGGATCAGTATGGCCTTTATGGCTACCATGAAGGGCTACAAAATGGTTCTAACTATGCCTTCTTATACAAGCTTGGAGAGGAGAGTTACAATGAGAGCATTTGGAGCTGACTTGATCCTCACTGATCCAACTAAAGGGATGGGAGGAACTGTCAAGAAGGCTTATGATCTTTTGGAATCCACACCCAATGGTTTCATGCTCCAACAATTCTCAAACCCTGCCAATACCCAGGTAATAATCTAATCCATTTTTCTTGGGGACTGTTATATTTTTGGATAAGTGAATTTAGCTGAAGTGGTCTAATGATTTTGTAGGAAGTGGTGTTTGGTTCACAAAATGTTTGATTTCAAAAGTGATCATAGAACTATCAAATTACTAAAACCCACCTCTCAAATAGTAACTATTTTCTAAGGGCCCATTTGGAACGTTCTTGTTTCTAGTTTATTGTTTACACTTTTATTATGAAACCTAAGTCTTTTGTAACTAATTATGTTTCTTGTTTCTCATTTTTTAGAAACGTTCTCAAAATTGTATAGAAATTTTGGAAACAATAAAAACAAGTTTCTTGTTTCTGCTTCCGTTTCTAATTTGTTATAAAATATAAATATAAAATAGTTTGATTTGTTTAATATTAAAAATGAGAAAACAGAAACAGGAAACAGAAATGTTAACAAACGGGCAATGATTCTTGGTCTATCACTCAACCAACAAGTGATTGATTTGGAATAGAATCATTTTGAAGTGATTTTGGACTACATTGTTATCAGACATCATTATTTTTTAAAAAAACATGCCCTTAGTTTCTATGTCTATTTTTCCAAATTATATTCGTCTAGCTCGATCCTAAGACGATTATTACAAAACGGAAAAAAACAGGTACATTTTGAAACGACTGGCCCAGAGATATGGGAGGATACAAATGGACAAGTTGACATCTTTGTGATGGGAATTGGCAGTGGAGGGACTGTCTCTGGGGTTGGACAGTATCTGAAATCTCGAAATCCCAATGTTAAGGTATGTGATTGACTATAAACTTTTGTTACCATAGAACACATATTAGAATTCATTGCCAATGGCTGAGTTTTTTTCTTCTTTACTGAAGATTTATGGAGTTGAGCCAGCTGAAAGCAATGTATTGAATGGTGGTAAACCAGGTATCATTTTATCGCTCCTTCGACGAAAATTCTCATCTTCTCCTCCTGAAAATGTCATTAGTTACGATAACTCTGACTCTAATCGAGGAAAACTGATCGCCTATGAAGTATAGACGATTTAGTTTAGGTAACTTGTCCATGTCTCAAACATTAAATGGAAAAAATTGAATAATGACAGGTAGGCTCGTGTGGAATACCATGGATAGACATAAATAGTAATACTACTGACAAAAATAATAAATTTTGAGAATGAAATATATGGCATATACTTTAAATTTCTTTCTGATATAGAAAAAAAGTATACTTAACATCACTGTGCCGTGTTGTGTCGTGTCGTGCTGGTTTGGGGATAACTAATGGACTGATCAGTCTACTTTGATAGCTACCTTTGTGGTAAAGATACAACTAATGGGGACTGGTTTTGTCAATTGGATTAGGTCCTCATCATATTACTGGCAATGGGGTTGGATTCAAGCCAGACATATTGGATATGGATGTAATGGATAAAGTTCTTGAAGTAAGTTGCTGCTAAAAAAGCCCATTTGGCTACAATTTTATTCTTTACTTTTTACATTATTGATATTTTTTCTTTTAAGAAAGTTCTTCTCTACGCATCGATGAGCAGGTGTTTTACTGACTTTTTGATGTGATGTATTTTTATTAGTCTATTTCTATTGTGTTTAATAGATCAATTAAGTGATTTGAATAAAAATTTTGAGACAATAAAAATAAAAATAGACCAATGAAAATACGTCATTGTCAAAAAGTCAGTAGGATATTATAATGACCAGAAGTGCTGTTCTATTTTTTCTCCCTAAAAACTAGATTTGAAAACAGCTAGTAATCAAATTTAGTTTCTTAAAGATAAAGTTGCGTTATCGAAGGGCTCTAAGGCTCCGTTAGATAACAATTTCATCTTTAGTTTTCTCGCAATTTATTTAGCATGCCTATTTCGATTCTAGAAAAATACCTTTCGAAAAAGAAATAAAGTTTTTGAAAACACCTTTTTTTTTTTTTTTTTTTTAGTTTTAAAAATTGACTTAGATTTTGAAAACATCCTTAAAAAGTAGATAATGAAAAATAGATGGGTAAAGTAGTATTTAAAAGCTTACTTTTCAAAAGCAGAGAATGAAAAACAAATTGCATTTTTCGTCTTGTGGATTCTATTCGTGTTTACATGTTATCAATAAACTTGGCAGGTAAGCAGTGAAGATGCTGTAAATATGGCCAGAGAATTGGCAGTGAAGGAGGGGCTAATGGTAAAAACAAAAATGAAAAGAAAACTCTCTTTTTCCTTCAAAACCATCTCATAAAACTTGATTGAAACAACATTTTTGCATCTTCCCCCCTTTTGTCTAAGATCATAGTTTCTCTTTTCTTTATCTTGATCATGCAGGTAGGAATATCGTCAGGGGCCAATACCGTCGCGGCGCTAAGGCTTGCGAGATTACCCGAAAACAAAGGCAAACTTATTGTGGTAAGCAAAAGCTTTACTCTCCTACATTCTTAAACAATCTCTAGATCCAAATCCATATTACATAAGATGAAGATCATTACTAGTTGTGAATGTCTTAAGTCATTTTACGAAGATGGTTCATCTTCGATATCAAGATAAGAAGTCGGTATAAGTTGTTATTTGGCTGCATTTTTTCAGACAATTCATCCAAGTTTTGGGGAGAGATACTTGTCATCTGTTCTGTTCCAAGAACTGAGGAATGAAGCTGAGAACATGCAACCGGTCTCAGTTGAC

mRNA sequence

ATGGCCGCCGCTGCTTCGAGAATTTTGCTGAAGCAGAGAGCCGCGATTTGGTCCGGCAGTTCCACGGTGAGGAAATTGTTTTCCACTCAAACGGTTCTCGATTCTCCTTCTTTCGCTCAGCGGCTCAGAGATCTGCCCAAGGATCTTCCTGGAACCAGTATCAGACGAGATGCTGCTCATCTCATTGGCAAAACTCCAATAGTATATCTTAACAAAGTCACTGAAGACTGTGGAGCTTATGTTGCTGTGAAGCAAGAGATGATGCAGCCTACTTCCAGCATTAAAGACAGACCGGCTTTCGCGATGATCACCGATGCTGAAAAGAAAGGTTTGATTACTCCAGGAAAGACGACATTGATCGAACCAACGTCGGGAAATATGGGGATCAGTATGGCCTTTATGGCTACCATGAAGGGCTACAAAATGGTTCTAACTATGCCTTCTTATACAAGCTTGGAGAGGAGAGTTACAATGAGAGCATTTGGAGCTGACTTGATCCTCACTGATCCAACTAAAGGGATGGGAGGAACTGTCAAGAAGGCTTATGATCTTTTGGAATCCACACCCAATGGTTTCATGCTCCAACAATTCTCAAACCCTGCCAATACCCAGGTACATTTTGAAACGACTGGCCCAGAGATATGGGAGGATACAAATGGACAAGTTGACATCTTTGTGATGGGAATTGGCAGTGGAGGGACTGTCTCTGGGGTTGGACAGTATCTGAAATCTCGAAATCCCAATGTTAAGATTTATGGAGTTGAGCCAGCTGAAAGCAATGTATTGAATGGTGGTAAACCAGGTCCTCATCATATTACTGGCAATGGGGTTGGATTCAAGCCAGACATATTGGATATGGATGTAATGGATAAAGTTCTTGAAGTAAGCAGTGAAGATGCTGTAAATATGGCCAGAGAATTGGCAGTGAAGGAGGGGCTAATGGTAGGAATATCGTCAGGGGCCAATACCGTCGCGGCGCTAAGGCTTGCGAGATTACCCGAAAACAAAGGCAAACTTATTGTGACAATTCATCCAAGTTTTGGGGAGAGATACTTGTCATCTGTTCTGTTCCAAGAACTGAGGAATGAAGCTGAGAACATGCAACCGGTCTCAGTTGAC

Coding sequence (CDS)

ATGGCCGCCGCTGCTTCGAGAATTTTGCTGAAGCAGAGAGCCGCGATTTGGTCCGGCAGTTCCACGGTGAGGAAATTGTTTTCCACTCAAACGGTTCTCGATTCTCCTTCTTTCGCTCAGCGGCTCAGAGATCTGCCCAAGGATCTTCCTGGAACCAGTATCAGACGAGATGCTGCTCATCTCATTGGCAAAACTCCAATAGTATATCTTAACAAAGTCACTGAAGACTGTGGAGCTTATGTTGCTGTGAAGCAAGAGATGATGCAGCCTACTTCCAGCATTAAAGACAGACCGGCTTTCGCGATGATCACCGATGCTGAAAAGAAAGGTTTGATTACTCCAGGAAAGACGACATTGATCGAACCAACGTCGGGAAATATGGGGATCAGTATGGCCTTTATGGCTACCATGAAGGGCTACAAAATGGTTCTAACTATGCCTTCTTATACAAGCTTGGAGAGGAGAGTTACAATGAGAGCATTTGGAGCTGACTTGATCCTCACTGATCCAACTAAAGGGATGGGAGGAACTGTCAAGAAGGCTTATGATCTTTTGGAATCCACACCCAATGGTTTCATGCTCCAACAATTCTCAAACCCTGCCAATACCCAGGTACATTTTGAAACGACTGGCCCAGAGATATGGGAGGATACAAATGGACAAGTTGACATCTTTGTGATGGGAATTGGCAGTGGAGGGACTGTCTCTGGGGTTGGACAGTATCTGAAATCTCGAAATCCCAATGTTAAGATTTATGGAGTTGAGCCAGCTGAAAGCAATGTATTGAATGGTGGTAAACCAGGTCCTCATCATATTACTGGCAATGGGGTTGGATTCAAGCCAGACATATTGGATATGGATGTAATGGATAAAGTTCTTGAAGTAAGCAGTGAAGATGCTGTAAATATGGCCAGAGAATTGGCAGTGAAGGAGGGGCTAATGGTAGGAATATCGTCAGGGGCCAATACCGTCGCGGCGCTAAGGCTTGCGAGATTACCCGAAAACAAAGGCAAACTTATTGTGACAATTCATCCAAGTTTTGGGGAGAGATACTTGTCATCTGTTCTGTTCCAAGAACTGAGGAATGAAGCTGAGAACATGCAACCGGTCTCAGTTGAC

Protein sequence

MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAHLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQELRNEAENMQPVSVD
Homology
BLAST of MS016959 vs. NCBI nr
Match: XP_022157695.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Momordica charantia])

HSP 1 Score: 729.6 bits (1882), Expect = 1.4e-206
Identity = 372/373 (99.73%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 60
           MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH
Sbjct: 1   MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 60

Query: 61  LIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 120
           LIGKTPIVYLNKVTEDCGAYVAVKQE+MQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI
Sbjct: 61  LIGKTPIVYLNKVTEDCGAYVAVKQEIMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 120

Query: 121 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 180
           EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK
Sbjct: 121 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 180

Query: 181 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 240
           AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ
Sbjct: 181 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 240

Query: 241 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 300
           YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA
Sbjct: 241 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 300

Query: 301 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 360
           VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL
Sbjct: 301 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 360

Query: 361 RNEAENMQPVSVD 374
           RNEAENMQPVSVD
Sbjct: 361 RNEAENMQPVSVD 373

BLAST of MS016959 vs. NCBI nr
Match: XP_022959417.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucurbita moschata] >KAG6575182.1 L-3-cyanoalanine synthase 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 694.1 bits (1790), Expect = 6.5e-196
Identity = 356/374 (95.19%), Postives = 365/374 (97.59%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGS-STVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AASR L+KQRAAI SG+ S++RKLFSTQTV DS SFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTE CG YVAVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGA+LI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240
           KAYDLLESTP+GFMLQQFSNPANTQVHF+TTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANTQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300
           QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED
Sbjct: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300

Query: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360
           AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE
Sbjct: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360

Query: 361 LRNEAENMQPVSVD 374
           LR EAENMQPVSVD
Sbjct: 361 LRKEAENMQPVSVD 374

BLAST of MS016959 vs. NCBI nr
Match: XP_023006594.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 693.3 bits (1788), Expect = 1.1e-195
Identity = 356/374 (95.19%), Postives = 365/374 (97.59%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGS-STVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AASR L+KQRAAI SG+ S++RKLFSTQTV DS SFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSMRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTE CG YVAVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGA+LI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240
           KAYDLLESTP+GFMLQQFSNPANTQVHF+TTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANTQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300
           QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED
Sbjct: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300

Query: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360
           AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE
Sbjct: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360

Query: 361 LRNEAENMQPVSVD 374
           LR EAENMQPVSVD
Sbjct: 361 LRKEAENMQPVSVD 374

BLAST of MS016959 vs. NCBI nr
Match: XP_023549039.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.8 bits (1784), Expect = 3.2e-195
Identity = 355/374 (94.92%), Postives = 365/374 (97.59%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGS-STVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AASR L+KQRAAI SG+ S++RKLFSTQTV DS SFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSMRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKV+E CG YVAVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVSEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGA+LI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240
           KAYDLLESTP+GFMLQQFSNPANTQVHF+TTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANTQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300
           QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED
Sbjct: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300

Query: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360
           AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE
Sbjct: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360

Query: 361 LRNEAENMQPVSVD 374
           LR EAENMQPVSVD
Sbjct: 361 LRKEAENMQPVSVD 374

BLAST of MS016959 vs. NCBI nr
Match: XP_038875773.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 690.3 bits (1780), Expect = 9.4e-195
Identity = 354/374 (94.65%), Postives = 363/374 (97.06%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWS-GSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60
           MAAAA R L+KQ+AAI S G STVRKLFSTQTVL+S SF QRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MAAAALRSLVKQKAAISSDGCSTVRKLFSTQTVLESSSFVQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120
           HLIGKTP+VYLNKVTE CGAY+AVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPMVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPAFAMINDAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMAT+KGYKMVLTMPSYTSLERRVTMRAFGA+LILTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATLKGYKMVLTMPSYTSLERRVTMRAFGAELILTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240
           KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300
           QYLKS+NPNVKIYGVEPAESNVLNGGKPGPH ITGNGVGFKPDILDMDVMDKVLEVSSED
Sbjct: 241 QYLKSKNPNVKIYGVEPAESNVLNGGKPGPHQITGNGVGFKPDILDMDVMDKVLEVSSED 300

Query: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360
           AVNMARELAVKEGLMVGISSGANT+AALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE
Sbjct: 301 AVNMARELAVKEGLMVGISSGANTIAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360

Query: 361 LRNEAENMQPVSVD 374
           LR EAENMQPVSVD
Sbjct: 361 LRKEAENMQPVSVD 374

BLAST of MS016959 vs. ExPASy Swiss-Prot
Match: Q1KLZ2 (L-3-cyanoalanine synthase 1, mitochondrial OS=Malus domestica OX=3750 GN=CAS1 PE=1 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.1e-174
Identity = 306/373 (82.04%), Postives = 340/373 (91.15%), Query Frame = 0

Query: 4   AASRILLKQRAAIWSGSSTVRKLFST---QTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 63
           AA R  LK+R+++    +  RKLFST   Q + DSPSFAQR+R+LPKDLPGT I+   + 
Sbjct: 2   AALRSFLKKRSSVLCNGAMRRKLFSTEVSQPLTDSPSFAQRIRNLPKDLPGTQIKTQVSQ 61

Query: 64  LIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 123
           LIG+TPIVYLNKVTE CGAY+AVKQEM QPT+SIKDRPA +MI DAE+KGLITPG+T LI
Sbjct: 62  LIGRTPIVYLNKVTEGCGAYIAVKQEMFQPTASIKDRPALSMINDAEEKGLITPGETILI 121

Query: 124 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 183
           EPTSGNMGISMAFMA M+GYKMVLTMPSYTSLERRV MR FGADLILTDPTKGMGGTVKK
Sbjct: 122 EPTSGNMGISMAFMAAMRGYKMVLTMPSYTSLERRVCMRCFGADLILTDPTKGMGGTVKK 181

Query: 184 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 243
           AYDLLESTPN +MLQQFSNPANT+VHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ
Sbjct: 182 AYDLLESTPNAYMLQQFSNPANTKVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 241

Query: 244 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 303
           YLKS+NPNV+IYGVEPAESNVLNGGKPGPH I GNGVGFKPDILD+D++++V+EV+SEDA
Sbjct: 242 YLKSKNPNVQIYGVEPAESNVLNGGKPGPHSIMGNGVGFKPDILDLDMLERVIEVTSEDA 301

Query: 304 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 363
           VNMAR+LA+KEGLMVGISSGANTVAA+ LA+ PENKGKLIVT+HPSFGERYLSSVLFQEL
Sbjct: 302 VNMARQLALKEGLMVGISSGANTVAAMELAKKPENKGKLIVTVHPSFGERYLSSVLFQEL 361

Query: 364 RNEAENMQPVSVD 374
           R EAENMQPV+VD
Sbjct: 362 RQEAENMQPVAVD 374

BLAST of MS016959 vs. ExPASy Swiss-Prot
Match: Q1KLZ1 (L-3-cyanoalanine synthase 2, mitochondrial OS=Malus domestica OX=3750 GN=CAS2 PE=1 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 1.4e-172
Identity = 306/374 (81.82%), Postives = 338/374 (90.37%), Query Frame = 0

Query: 4   AASRILLKQRAAIWSGSSTVR-KLFS---TQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 63
           AA R  LK+R+++      VR +LFS   +Q + DSPSFAQR+ +LPKDLP T I+   +
Sbjct: 2   AALRSFLKKRSSVLCNGGAVRHRLFSAQVSQPIPDSPSFAQRIGNLPKDLPATQIKTQVS 61

Query: 64  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 123
            LIG+TPIVYLNKVTE CGA++AVKQEM QPT+SIKDRPA +MI DAE+KGLITPG+TTL
Sbjct: 62  QLIGRTPIVYLNKVTEGCGAFIAVKQEMFQPTASIKDRPALSMINDAEEKGLITPGETTL 121

Query: 124 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 183
           IEPTSGNMGISMAFMA MKGYKMVLTMPSYTSLERRV MR FGADLILTDPTKGMGGTVK
Sbjct: 122 IEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVCMRCFGADLILTDPTKGMGGTVK 181

Query: 184 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 243
           KAYDLLESTPN FMLQQFSNPANT+VHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 182 KAYDLLESTPNAFMLQQFSNPANTKVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 241

Query: 244 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 303
           QYLKS+NPNV+IYGVEPAESNVLNGGKPGPH ITGNGVGFKPDILDMD+M++V+EV SED
Sbjct: 242 QYLKSKNPNVQIYGVEPAESNVLNGGKPGPHSITGNGVGFKPDILDMDMMERVIEVRSED 301

Query: 304 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 363
           AVNMAR+LA+KEGLMVGISSGANTVAA+ LA+ PENKGKLIVT+H SFGERYLSSVLFQ+
Sbjct: 302 AVNMARQLALKEGLMVGISSGANTVAAMELAKKPENKGKLIVTVHASFGERYLSSVLFQD 361

Query: 364 LRNEAENMQPVSVD 374
           LR EAENMQPV+VD
Sbjct: 362 LRQEAENMQPVAVD 375

BLAST of MS016959 vs. ExPASy Swiss-Prot
Match: Q76MX2 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial OS=Solanum tuberosum OX=4113 GN=PCAS-1 PE=1 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 7.5e-171
Identity = 297/350 (84.86%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 24  RKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAHLIGKTPIVYLNKVTEDCGAYVAV 83
           R+ +S+++     SFAQRLRDLPK LPGT+I+   + LIGKTP+VYLNKV+E CGAY+AV
Sbjct: 7   RRFYSSES-----SFAQRLRDLPKYLPGTNIKTQVSQLIGKTPLVYLNKVSEGCGAYIAV 66

Query: 84  KQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMV 143
           KQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTLIEPTSGNMGISMAFMA MKGYKM+
Sbjct: 67  KQEMMQPTSSIKDRPAFAMINDAEKKGLITPGKTTLIEPTSGNMGISMAFMAAMKGYKMI 126

Query: 144 LTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKKAYDLLESTPNGFMLQQFSNPANT 203
           LTMPSYTSLERRVTMRAFGADL+ TDPTKGMGGT+KKAYDLLESTPN +MLQQFSNPANT
Sbjct: 127 LTMPSYTSLERRVTMRAFGADLVTTDPTKGMGGTIKKAYDLLESTPNAYMLQQFSNPANT 186

Query: 204 QVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLN 263
           Q HFETTGPEIWEDT G VDIFVMGIGSGGTVSGVGQYLKS+NPNVKIYG+EP ESNVLN
Sbjct: 187 QAHFETTGPEIWEDTQGNVDIFVMGIGSGGTVSGVGQYLKSKNPNVKIYGIEPTESNVLN 246

Query: 264 GGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANT 323
           GG PGPH ITGNGVGFKPDILDMDVM++VL VSSE++VNMARELA+KEGLMVGISSGANT
Sbjct: 247 GGNPGPHEITGNGVGFKPDILDMDVMEEVLMVSSEESVNMARELALKEGLMVGISSGANT 306

Query: 324 VAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQELRNEAENMQPVSVD 374
           VAALRLA  PENKGKLIVTIHPSFGERYLSSVL++++R EA+NMQPVSVD
Sbjct: 307 VAALRLANRPENKGKLIVTIHPSFGERYLSSVLYEDIRKEAQNMQPVSVD 351

BLAST of MS016959 vs. ExPASy Swiss-Prot
Match: Q9S757 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase C1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CYSC1 PE=1 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 1.5e-166
Identity = 298/373 (79.89%), Postives = 332/373 (89.01%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 60
           MA+ + R+L  +R  I   S TVRKLFST   + SPSFAQRLRDLPKD P T+ +RDA+ 
Sbjct: 1   MASVSRRLL--RRETIPCFSHTVRKLFST---VGSPSFAQRLRDLPKDFPSTNAKRDASL 60

Query: 61  LIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 120
           LIGKTP+V+LNKVTE C AYVA KQE  QPT SIKDRPA AMI DAEKK LI PGKTTLI
Sbjct: 61  LIGKTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLI 120

Query: 121 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 180
           EPTSGNMGIS+AFMA MKGY++++TMPSYTSLERRVTMR+FGA+L+LTDP KGMGGTVKK
Sbjct: 121 EPTSGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKK 180

Query: 181 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 240
           AYDLL+STP+ FM QQF+NPANTQ+HF+TTGPEIWEDT G VDIFVMGIGSGGTVSGVG+
Sbjct: 181 AYDLLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGR 240

Query: 241 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 300
           YLKS+NPNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP+ILDMDVM+ VLEVSSEDA
Sbjct: 241 YLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEILDMDVMESVLEVSSEDA 300

Query: 301 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 360
           + MARELA+KEGLMVGISSGANTVAA+RLA++PENKGKLIVTIH SFGERYLSSVLF EL
Sbjct: 301 IKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGERYLSSVLFDEL 360

Query: 361 RNEAENMQPVSVD 374
           R EAE M+PVSVD
Sbjct: 361 RKEAEEMKPVSVD 368

BLAST of MS016959 vs. ExPASy Swiss-Prot
Match: Q9FS29 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial OS=Solanum tuberosum OX=4113 GN=PCAS-2 PE=1 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 2.1e-160
Identity = 281/325 (86.46%), Postives = 304/325 (93.54%), Query Frame = 0

Query: 49  LPGTSIRRDAAHLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEK 108
           LP T+I+ + + LIGKTP+VYL KVTE CGAY+AVKQEM QPTSSIKDRPA AMI DAEK
Sbjct: 23  LPHTNIKSEVSQLIGKTPMVYLKKVTEGCGAYIAVKQEMFQPTSSIKDRPALAMINDAEK 82

Query: 109 KGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILT 168
           KGLI+P KTTLIEPTSGNMGISMAFMA MKGYKMVLTMPSYTS+ERRVTMRAFGADLILT
Sbjct: 83  KGLISPEKTTLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVTMRAFGADLILT 142

Query: 169 DPTKGMGGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMG 228
           DPTKGMGGTVKKAY+LLESTPN FMLQQFSNPANTQVHF+TTGPEIWE+T G VDIFVMG
Sbjct: 143 DPTKGMGGTVKKAYELLESTPNAFMLQQFSNPANTQVHFDTTGPEIWEETLGNVDIFVMG 202

Query: 229 IGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDV 288
           IGSGGTV+GVG YLKS+NPNVKIYG+EP ESN+LNGGKPGPHHITGNGVG KPDI+DMD+
Sbjct: 203 IGSGGTVTGVGLYLKSKNPNVKIYGLEPTESNILNGGKPGPHHITGNGVGSKPDIVDMDL 262

Query: 289 MDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFG 348
           M++VL VSSEDAVNMARELAVKEGLMVGISSGANTVAALRLA+ PENKGKLIVT+H SFG
Sbjct: 263 MEEVLMVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLAQKPENKGKLIVTVHASFG 322

Query: 349 ERYLSSVLFQELRNEAENMQPVSVD 374
           ERYLSSVL+Q+LR EAENMQPVSVD
Sbjct: 323 ERYLSSVLYQDLRKEAENMQPVSVD 347

BLAST of MS016959 vs. ExPASy TrEMBL
Match: A0A6J1DTT8 (Cysteine synthase OS=Momordica charantia OX=3673 GN=LOC111024353 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 6.8e-207
Identity = 372/373 (99.73%), Postives = 373/373 (100.00%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 60
           MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH
Sbjct: 1   MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 60

Query: 61  LIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 120
           LIGKTPIVYLNKVTEDCGAYVAVKQE+MQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI
Sbjct: 61  LIGKTPIVYLNKVTEDCGAYVAVKQEIMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 120

Query: 121 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 180
           EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK
Sbjct: 121 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 180

Query: 181 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 240
           AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ
Sbjct: 181 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 240

Query: 241 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 300
           YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA
Sbjct: 241 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 300

Query: 301 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 360
           VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL
Sbjct: 301 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 360

Query: 361 RNEAENMQPVSVD 374
           RNEAENMQPVSVD
Sbjct: 361 RNEAENMQPVSVD 373

BLAST of MS016959 vs. ExPASy TrEMBL
Match: A0A6J1H4G6 (Cysteine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460401 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.2e-196
Identity = 356/374 (95.19%), Postives = 365/374 (97.59%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGS-STVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AASR L+KQRAAI SG+ S++RKLFSTQTV DS SFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTE CG YVAVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGA+LI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240
           KAYDLLESTP+GFMLQQFSNPANTQVHF+TTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANTQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300
           QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED
Sbjct: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300

Query: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360
           AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE
Sbjct: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360

Query: 361 LRNEAENMQPVSVD 374
           LR EAENMQPVSVD
Sbjct: 361 LRKEAENMQPVSVD 374

BLAST of MS016959 vs. ExPASy TrEMBL
Match: A0A6J1L2L7 (Cysteine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499275 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 5.4e-196
Identity = 356/374 (95.19%), Postives = 365/374 (97.59%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGS-STVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AASR L+KQRAAI SG+ S++RKLFSTQTV DS SFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSMRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTE CG YVAVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGA+LI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240
           KAYDLLESTP+GFMLQQFSNPANTQVHF+TTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANTQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVG 240

Query: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300
           QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED
Sbjct: 241 QYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSED 300

Query: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360
           AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE
Sbjct: 301 AVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQE 360

Query: 361 LRNEAENMQPVSVD 374
           LR EAENMQPVSVD
Sbjct: 361 LRKEAENMQPVSVD 374

BLAST of MS016959 vs. ExPASy TrEMBL
Match: A0A0A0K9Z6 (Cysteine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G004600 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.3e-193
Identity = 350/375 (93.33%), Postives = 363/375 (96.80%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWS-GSSTVRKLFSTQTVLD-SPSFAQRLRDLPKDLPGTSIRRDA 60
           MAAAA R L+KQ+A I S G STVRK FSTQTV + S SFAQRLRDLPKDLPGTS+RRDA
Sbjct: 1   MAAAALRTLVKQKAGISSDGCSTVRKFFSTQTVFESSSSFAQRLRDLPKDLPGTSVRRDA 60

Query: 61  AHLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTT 120
           AHLIGKTP+VYLNKVTE CGAY+AVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTT
Sbjct: 61  AHLIGKTPLVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPAFAMINDAEKKGLITPGKTT 120

Query: 121 LIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTV 180
           LIEPTSGNMGISMAFMAT+KGYKMVLTMPSYTSLERRVTMRAFGA+LILTDPTKGMGGTV
Sbjct: 121 LIEPTSGNMGISMAFMATLKGYKMVLTMPSYTSLERRVTMRAFGAELILTDPTKGMGGTV 180

Query: 181 KKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGV 240
           KKAYDLLESTP+GFMLQQF+NPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGV
Sbjct: 181 KKAYDLLESTPDGFMLQQFANPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGV 240

Query: 241 GQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSE 300
           GQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSS+
Sbjct: 241 GQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSD 300

Query: 301 DAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQ 360
           DAVNMARELAVKEGLMVGISSGANTVAALRLA+LPENKGKLIVT+HPSFGERYLSSVLFQ
Sbjct: 301 DAVNMARELAVKEGLMVGISSGANTVAALRLAKLPENKGKLIVTVHPSFGERYLSSVLFQ 360

Query: 361 ELRNEAENMQPVSVD 374
           ELRNEAENMQPVSVD
Sbjct: 361 ELRNEAENMQPVSVD 375

BLAST of MS016959 vs. ExPASy TrEMBL
Match: A0A5D3BW04 (Cysteine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001170 PE=3 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 1.2e-192
Identity = 351/379 (92.61%), Postives = 363/379 (95.78%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSG--SSTVRKLFSTQTVLD----SPSFAQRLRDLPKDLPGTSI 60
           MAAAA R L+KQ+A I S   S+TVRKLFSTQTVLD    S SFAQRLRDLPKDLPGTS+
Sbjct: 1   MAAAALRSLVKQKAGISSDGCSTTVRKLFSTQTVLDSSSSSSSFAQRLRDLPKDLPGTSV 60

Query: 61  RRDAAHLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITP 120
           RRDAAHLIGKTP+VYLNKVTE CGAY+AVKQEMMQPTSSIKDRPAFAMI DAEKKGLITP
Sbjct: 61  RRDAAHLIGKTPLVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPAFAMINDAEKKGLITP 120

Query: 121 GKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGM 180
           G+TTLIEPTSGNMGISMAFMAT+KGYKMVLTMPSYTSLERRVTMRAFGA+LILTDPTKGM
Sbjct: 121 GQTTLIEPTSGNMGISMAFMATLKGYKMVLTMPSYTSLERRVTMRAFGAELILTDPTKGM 180

Query: 181 GGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGT 240
           GGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNG VDIFVMGIGSGGT
Sbjct: 181 GGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGLVDIFVMGIGSGGT 240

Query: 241 VSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLE 300
           VSGVGQYLKSRNPNVKIYGVEPAESN+LNGGKPGPHHITGNGVGFKPDILDMDVMDKVLE
Sbjct: 241 VSGVGQYLKSRNPNVKIYGVEPAESNILNGGKPGPHHITGNGVGFKPDILDMDVMDKVLE 300

Query: 301 VSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSS 360
           VSSEDAVNMARELAVKEGLMVGISSGANTVAALRLA+LPENKGKLIVT+HPSFGERYLSS
Sbjct: 301 VSSEDAVNMARELAVKEGLMVGISSGANTVAALRLAKLPENKGKLIVTVHPSFGERYLSS 360

Query: 361 VLFQELRNEAENMQPVSVD 374
           VLFQELR EAENMQPVSVD
Sbjct: 361 VLFQELRKEAENMQPVSVD 379

BLAST of MS016959 vs. TAIR 10
Match: AT3G61440.1 (cysteine synthase C1 )

HSP 1 Score: 587.0 bits (1512), Expect = 1.0e-167
Identity = 298/373 (79.89%), Postives = 332/373 (89.01%), Query Frame = 0

Query: 1   MAAAASRILLKQRAAIWSGSSTVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAAH 60
           MA+ + R+L  +R  I   S TVRKLFST   + SPSFAQRLRDLPKD P T+ +RDA+ 
Sbjct: 1   MASVSRRLL--RRETIPCFSHTVRKLFST---VGSPSFAQRLRDLPKDFPSTNAKRDASL 60

Query: 61  LIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKGLITPGKTTLI 120
           LIGKTP+V+LNKVTE C AYVA KQE  QPT SIKDRPA AMI DAEKK LI PGKTTLI
Sbjct: 61  LIGKTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLI 120

Query: 121 EPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKK 180
           EPTSGNMGIS+AFMA MKGY++++TMPSYTSLERRVTMR+FGA+L+LTDP KGMGGTVKK
Sbjct: 121 EPTSGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKK 180

Query: 181 AYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQ 240
           AYDLL+STP+ FM QQF+NPANTQ+HF+TTGPEIWEDT G VDIFVMGIGSGGTVSGVG+
Sbjct: 181 AYDLLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGR 240

Query: 241 YLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDA 300
           YLKS+NPNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP+ILDMDVM+ VLEVSSEDA
Sbjct: 241 YLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEILDMDVMESVLEVSSEDA 300

Query: 301 VNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQEL 360
           + MARELA+KEGLMVGISSGANTVAA+RLA++PENKGKLIVTIH SFGERYLSSVLF EL
Sbjct: 301 IKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGERYLSSVLFDEL 360

Query: 361 RNEAENMQPVSVD 374
           R EAE M+PVSVD
Sbjct: 361 RKEAEEMKPVSVD 368

BLAST of MS016959 vs. TAIR 10
Match: AT3G61440.2 (cysteine synthase C1 )

HSP 1 Score: 474.9 bits (1221), Expect = 5.8e-134
Identity = 232/272 (85.29%), Postives = 256/272 (94.12%), Query Frame = 0

Query: 102 MITDAEKKGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAF 161
           MI DAEKK LI PGKTTLIEPTSGNMGIS+AFMA MKGY++++TMPSYTSLERRVTMR+F
Sbjct: 1   MIADAEKKKLIIPGKTTLIEPTSGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSF 60

Query: 162 GADLILTDPTKGMGGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQ 221
           GA+L+LTDP KGMGGTVKKAYDLL+STP+ FM QQF+NPANTQ+HF+TTGPEIWEDT G 
Sbjct: 61  GAELVLTDPAKGMGGTVKKAYDLLDSTPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGN 120

Query: 222 VDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKP 281
           VDIFVMGIGSGGTVSGVG+YLKS+NPNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP
Sbjct: 121 VDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKP 180

Query: 282 DILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIV 341
           +ILDMDVM+ VLEVSSEDA+ MARELA+KEGLMVGISSGANTVAA+RLA++PENKGKLIV
Sbjct: 181 EILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIV 240

Query: 342 TIHPSFGERYLSSVLFQELRNEAENMQPVSVD 374
           TIH SFGERYLSSVLF ELR EAE M+PVSVD
Sbjct: 241 TIHASFGERYLSSVLFDELRKEAEEMKPVSVD 272

BLAST of MS016959 vs. TAIR 10
Match: AT3G61440.3 (cysteine synthase C1 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.5e-121
Identity = 210/247 (85.02%), Postives = 234/247 (94.74%), Query Frame = 0

Query: 127 MGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKKAYDLLE 186
           MGIS+AFMA MKGY++++TMPSYTSLERRVTMR+FGA+L+LTDP KGMGGTVKKAYDLL+
Sbjct: 1   MGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYDLLD 60

Query: 187 STPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRN 246
           STP+ FM QQF+NPANTQ+HF+TTGPEIWEDT G VDIFVMGIGSGGTVSGVG+YLKS+N
Sbjct: 61  STPDAFMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLKSKN 120

Query: 247 PNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVNMARE 306
           PNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP+ILDMDVM+ VLEVSSEDA+ MARE
Sbjct: 121 PNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEILDMDVMESVLEVSSEDAIKMARE 180

Query: 307 LAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQELRNEAEN 366
           LA+KEGLMVGISSGANTVAA+RLA++PENKGKLIVTIH SFGERYLSSVLF ELR EAE 
Sbjct: 181 LALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGERYLSSVLFDELRKEAEE 240

Query: 367 MQPVSVD 374
           M+PVSVD
Sbjct: 241 MKPVSVD 247

BLAST of MS016959 vs. TAIR 10
Match: AT2G43750.1 (O-acetylserine (thiol) lyase B )

HSP 1 Score: 407.9 bits (1047), Expect = 8.7e-114
Identity = 190/319 (59.56%), Postives = 253/319 (79.31%), Query Frame = 0

Query: 51  GTSIRRDAAHLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKG 110
           G +I  +AA LIGKTP+VYLN V + C A VA K E+M+P  S+KDR  ++MITDAE+KG
Sbjct: 72  GLNIADNAAQLIGKTPMVYLNNVVKGCVASVAAKLEIMEPCCSVKDRIGYSMITDAEEKG 131

Query: 111 LITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDP 170
           LITPGK+ L+E TSGN GI +AF+A  KGYK++LTMP+  SLERRV +RAFGA+L+LT+P
Sbjct: 132 LITPGKSVLVESTSGNTGIGLAFIAASKGYKLILTMPASMSLERRVLLRAFGAELVLTEP 191

Query: 171 TKGMGGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIG 230
            KGM G ++KA ++L+ TPN +MLQQF NPAN ++H+ETTGPEIWEDT G++DI V GIG
Sbjct: 192 AKGMTGAIQKAEEILKKTPNSYMLQQFDNPANPKIHYETTGPEIWEDTRGKIDILVAGIG 251

Query: 231 SGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMD 290
           +GGT++GVG+++K R P +K+ GVEP ES +L+GGKPGPH I G G GF P  LD+ ++D
Sbjct: 252 TGGTITGVGRFIKERKPELKVIGVEPTESAILSGGKPGPHKIQGIGAGFVPKNLDLAIVD 311

Query: 291 KVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGER 350
           + + +SSE+A+  +++LA++EGL+VGISSGA   AA+++A+ PEN GKLI  + PSFGER
Sbjct: 312 EYIAISSEEAIETSKQLALQEGLLVGISSGAAAAAAIQVAKRPENAGKLIAVVFPSFGER 371

Query: 351 YLSSVLFQELRNEAENMQP 370
           YLS+ LFQ +R E E MQP
Sbjct: 372 YLSTQLFQSIREECEQMQP 390

BLAST of MS016959 vs. TAIR 10
Match: AT2G43750.2 (O-acetylserine (thiol) lyase B )

HSP 1 Score: 407.9 bits (1047), Expect = 8.7e-114
Identity = 190/319 (59.56%), Postives = 253/319 (79.31%), Query Frame = 0

Query: 51  GTSIRRDAAHLIGKTPIVYLNKVTEDCGAYVAVKQEMMQPTSSIKDRPAFAMITDAEKKG 110
           G +I  +AA LIGKTP+VYLN V + C A VA K E+M+P  S+KDR  ++MITDAE+KG
Sbjct: 72  GLNIADNAAQLIGKTPMVYLNNVVKGCVASVAAKLEIMEPCCSVKDRIGYSMITDAEEKG 131

Query: 111 LITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDP 170
           LITPGK+ L+E TSGN GI +AF+A  KGYK++LTMP+  SLERRV +RAFGA+L+LT+P
Sbjct: 132 LITPGKSVLVESTSGNTGIGLAFIAASKGYKLILTMPASMSLERRVLLRAFGAELVLTEP 191

Query: 171 TKGMGGTVKKAYDLLESTPNGFMLQQFSNPANTQVHFETTGPEIWEDTNGQVDIFVMGIG 230
            KGM G ++KA ++L+ TPN +MLQQF NPAN ++H+ETTGPEIWEDT G++DI V GIG
Sbjct: 192 AKGMTGAIQKAEEILKKTPNSYMLQQFDNPANPKIHYETTGPEIWEDTRGKIDILVAGIG 251

Query: 231 SGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMD 290
           +GGT++GVG+++K R P +K+ GVEP ES +L+GGKPGPH I G G GF P  LD+ ++D
Sbjct: 252 TGGTITGVGRFIKERKPELKVIGVEPTESAILSGGKPGPHKIQGIGAGFVPKNLDLAIVD 311

Query: 291 KVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGER 350
           + + +SSE+A+  +++LA++EGL+VGISSGA   AA+++A+ PEN GKLI  + PSFGER
Sbjct: 312 EYIAISSEEAIETSKQLALQEGLLVGISSGAAAAAAIQVAKRPENAGKLIAVVFPSFGER 371

Query: 351 YLSSVLFQELRNEAENMQP 370
           YLS+ LFQ +R E E MQP
Sbjct: 372 YLSTQLFQSIREECEQMQP 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157695.11.4e-20699.73bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Momor... [more]
XP_022959417.16.5e-19695.19bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucur... [more]
XP_023006594.11.1e-19595.19bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucur... [more]
XP_023549039.13.2e-19594.92bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucur... [more]
XP_038875773.19.4e-19594.65bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Benin... [more]
Match NameE-valueIdentityDescription
Q1KLZ21.1e-17482.04L-3-cyanoalanine synthase 1, mitochondrial OS=Malus domestica OX=3750 GN=CAS1 PE... [more]
Q1KLZ11.4e-17281.82L-3-cyanoalanine synthase 2, mitochondrial OS=Malus domestica OX=3750 GN=CAS2 PE... [more]
Q76MX27.5e-17184.86Bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial OS=Sol... [more]
Q9S7571.5e-16679.89Bifunctional L-3-cyanoalanine synthase/cysteine synthase C1, mitochondrial OS=Ar... [more]
Q9FS292.1e-16086.46Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial OS=Sol... [more]
Match NameE-valueIdentityDescription
A0A6J1DTT86.8e-20799.73Cysteine synthase OS=Momordica charantia OX=3673 GN=LOC111024353 PE=3 SV=1[more]
A0A6J1H4G63.2e-19695.19Cysteine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460401 PE=3 SV=1[more]
A0A6J1L2L75.4e-19695.19Cysteine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499275 PE=3 SV=1[more]
A0A0A0K9Z63.3e-19393.33Cysteine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G004600 PE=3 SV=1[more]
A0A5D3BW041.2e-19292.61Cysteine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001... [more]
Match NameE-valueIdentityDescription
AT3G61440.11.0e-16779.89cysteine synthase C1 [more]
AT3G61440.25.8e-13485.29cysteine synthase C1 [more]
AT3G61440.31.5e-12185.02cysteine synthase C1 [more]
AT2G43750.18.7e-11459.56O-acetylserine (thiol) lyase B [more]
AT2G43750.28.7e-11459.56O-acetylserine (thiol) lyase B [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 61..353
e-value: 8.4E-124
score: 414.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 96..198
e-value: 8.4E-124
score: 414.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 56..361
IPR005859Cysteine synthase CysKTIGRFAMTIGR01139TIGR01139coord: 60..357
e-value: 1.0E-124
score: 413.6
IPR005856Cysteine synthaseTIGRFAMTIGR01136TIGR01136coord: 60..357
e-value: 2.9E-123
score: 409.0
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 61..345
e-value: 1.6E-66
score: 224.7
IPR031111L-3-cyanoalanine synthase, mitochondrialPANTHERPTHR10314:SF80BIFUNCTIONAL L-3-CYANOALANINE SYNTHASE/CYSTEINE SYNTHASE C1, MITOCHONDRIALcoord: 21..372
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 21..372
NoneNo IPR availableCDDcd01561CBS_likecoord: 63..353
e-value: 2.16001E-144
score: 408.826
IPR001216Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment sitePROSITEPS00901CYS_SYNTHASEcoord: 84..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS016959.1MS016959.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019499 cyanide metabolic process
biological_process GO:0006535 cysteine biosynthetic process from serine
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004124 cysteine synthase activity
molecular_function GO:0050017 L-3-cyanoalanine synthase activity