MS016951 (gene) Bitter gourd (TR) v1

BLAST of MS016951 vs. NCBI nr
Match: XP_022157677.1 (U-box domain-containing protein 33-like [Momordica charantia])

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 722/810 (89.14%), Postives = 750/810 (92.59%), Query Frame = 0

Query: 7   VVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEVKA 66
           VVYVAVGKDIK+C+LNLVYA Q+SGGRRICILH+HEP KMI I   K PASSMKEEEV A
Sbjct: 16  VVYVAVGKDIKECKLNLVYAAQNSGGRRICILHIHEPPKMIRISGKKVPASSMKEEEVNA 75

Query: 67  YRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAAAD 126
            RELERQD HKILDGFLLLC  AGARAEKLYFES+K+K+G+V+LISL GIR+L+MGAAAD
Sbjct: 76  CRELERQDIHKILDGFLLLCRVAGARAEKLYFESDKIKEGLVKLISLCGIRKLVMGAAAD 135

Query: 127 KHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADI----PLLSP 186
           KHYS RM KIRSRKA YVHL APAFCQIQFVCKG L  IREACPRE+HA+I    PL  P
Sbjct: 136 KHYSTRMKKIRSRKAMYVHLKAPAFCQIQFVCKGMLIRIREACPREAHANIPLPLPLPLP 195

Query: 187 QTHN-INDASRRPVQSAQFNGSRIDSPSRL----SISNSMSDASGTWSPFEHLYSTSSYS 246
           QT N IN AS R VQS Q NGSR D+PS +    SISNSM DA G+WSPFEH YSTSS S
Sbjct: 196 QTQNIINGASWRSVQSGQSNGSRGDTPSIVLETRSISNSMLDA-GSWSPFEHPYSTSSCS 255

Query: 247 GCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQA 306
           GC++V SSRTEEDEYGLGL+ PL++ DL PN   P+L    GF+QD SVDDSLYI+LEQA
Sbjct: 256 GCINVPSSRTEEDEYGLGLNLPLQMKDLTPNLYSPNLL---GFEQDESVDDSLYIRLEQA 315

Query: 307 ITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGEL 366
           ITD ANA+++AF+EA RR KAEKEL DAI RVKDSEALYAGESRERKEIEAALSKERGEL
Sbjct: 316 ITDAANAKREAFKEATRRIKAEKELGDAIRRVKDSEALYAGESRERKEIEAALSKERGEL 375

Query: 367 DNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELL 426
           DNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELL
Sbjct: 376 DNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELL 435

Query: 427 RQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYK 486
           RQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYK
Sbjct: 436 RQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYK 495

Query: 487 GLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANG 546
           GLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANG
Sbjct: 496 GLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANG 555

Query: 547 SLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIP 606
           SLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIP
Sbjct: 556 SLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIP 615

Query: 607 KLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLH 666
           KLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLH
Sbjct: 616 KLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLH 675

Query: 667 EVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMR 726
           EVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMR
Sbjct: 676 EVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMR 735

Query: 727 ASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT 786
           ASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT
Sbjct: 736 ASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT 795

Query: 787 SPMTNLRLDHRNLVPNRALRSAIQEWLHQP 808
           SPMTNLRLDHRNLVPNRALRSAIQEWLHQP
Sbjct: 796 SPMTNLRLDHRNLVPNRALRSAIQEWLHQP 821

BLAST of MS016951 vs. NCBI nr
Match: XP_038907095.1 (U-box domain-containing protein 33-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 631/819 (77.05%), Postives = 704/819 (85.96%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS+   VVYVAVG DIK C+LNLVYAI+ SGGR+ICILHVHEPAK+IPIL TKF ASSMK
Sbjct: 3   GSSEEDVVYVAVGNDIKACKLNLVYAIKQSGGRKICILHVHEPAKLIPILGTKFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           EE+V AYRELERQDA+KILDGFLLLCHQAGARAEKLYFES+K+KKGIVEL+SL GIRRLI
Sbjct: 63  EEKVNAYRELERQDANKILDGFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRRLI 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK++SR+MTKIRSRKA YVHLNAPAFCQIQFVC GQL  IREACP+E HADI L 
Sbjct: 123 MGAAADKYHSRKMTKIRSRKAMYVHLNAPAFCQIQFVCNGQLIRIREACPQEPHADISLP 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ NIN AS RPVQS QFNG  IDSPS    RL+IS+++ + SGTWS FEH YSTS  
Sbjct: 183 SPQSQNINGASWRPVQSGQFNGRIIDSPSIGMERLTISDAILNTSGTWSAFEHSYSTSPS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGCMDVASSRTEEDE+ LGL SP +L + APN+SPP L    GFQQDGS DDSLYIQLE+
Sbjct: 243 SGCMDVASSRTEEDEFELGLDSPRQLTNFAPNSSPPHLL---GFQQDGSADDSLYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           AITD ANAR++AFREA++R KAE EL  AI R K +EALYA E R RKE E A SKER E
Sbjct: 303 AITDAANARREAFREALKRAKAEIELGHAIRRAKVAEALYAEEFRGRKETEEARSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           LD+VKNQVNEM +ELQIARNKGLKLENQIAESD MVKELE+KILSAIELLHNYKNDRDEL
Sbjct: 363 LDDVKNQVNEMTKELQIARNKGLKLENQIAESDEMVKELEQKILSAIELLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKE+ D+RTRQ +A  +HSA    EFSFSEIAEATRKFDPSLKI + T+G +Y
Sbjct: 423 LKQRDEALKELNDIRTRQVEARIQHSAH---EFSFSEIAEATRKFDPSLKIVEGTNGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPL-AFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFA 540
           KGLLYNTEV+IKML SH+LQNP   F+REVDVL+KLRHPNI TLIG C E+CILVYDYF 
Sbjct: 483 KGLLYNTEVSIKMLCSHNLQNPEDEFQREVDVLSKLRHPNIATLIGVCPEACILVYDYFP 542

Query: 541 NGSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNY 600
           NG+LEDRLACKDNSSPLSWKTRIRIAT+LCSAL+F+H+NK+C++IH D+ P+N+LLD NY
Sbjct: 543 NGNLEDRLACKDNSSPLSWKTRIRIATELCSALIFIHSNKVCKIIHGDMKPSNVLLDANY 602

Query: 601 IPKLAGFGNCHFLPNDETTSVRADI------ESNHESPLTTKLDVFSFGIVLLRLLTGKS 660
           +PKLAGFG CHFLP+DE +S   D+      + NHE PLTT+LDVFSFG++LL LLTG+S
Sbjct: 603 VPKLAGFGTCHFLPHDEKSSYNEDLSAGYDAKGNHEFPLTTELDVFSFGMILLSLLTGQS 662

Query: 661 DMMIKLLHEVQFAID--GRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLAS 720
              ++L  +VQF I+   RKLKDVLDPRAGDWPFVQAEQLAQLA RCCNTNSM RPDL S
Sbjct: 663 --YLRLKEDVQFIINIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTNSMYRPDLVS 722

Query: 721 DVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEAL 780
           DVWRVLEPMRASCG S SVCL  G+QQ+QPPPYFICPIFQEVMEDPHVAADGFTYEAEAL
Sbjct: 723 DVWRVLEPMRASCGGSQSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEAL 782

Query: 781 RGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           RGWLDSGHDTSPMTNLRLDH+NLVPNRALRS IQEWL Q
Sbjct: 783 RGWLDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 813

BLAST of MS016951 vs. NCBI nr
Match: XP_023547655.1 (U-box domain-containing protein 33-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 613/816 (75.12%), Postives = 702/816 (86.03%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS G+ VVY+AVG D+ +C LNLVYAI+H GGRRICILHVHEPAK+IP L T+F ASSMK
Sbjct: 3   GSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICILHVHEPAKLIPFLGTQFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           E+EV AYRE ERQDA+KILDGFL LCHQAG RAEKLY ES+K+KKGIVEL+SL GIRRLI
Sbjct: 63  EKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYVESDKIKKGIVELVSLHGIRRLI 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRS+KA YVHL APAFCQIQFVC GQL   REACP+E+H DIP  
Sbjct: 123 MGAAADKYYSRKMTKIRSKKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPP 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ N+N A  RPV+S QFNGSRI SPS    RLSIS  +S+ SGT SPFE  YS SS 
Sbjct: 183 SPQSQNVNGACWRPVESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGC+D ASSRTEEDEYG+GLSSPL L D+APN+SP  LS   GFQQD S DD LYIQLE+
Sbjct: 243 SGCLDAASSRTEEDEYGVGLSSPLLLKDVAPNSSPLHLS---GFQQDESADDILYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           +IT+ ANA+++AFREA++R +AE EL +AI R K  E LYAGE+RERK++E ALSKER E
Sbjct: 303 SITEAANAKREAFREAVKRAQAENELGNAIFRAKFYEDLYAGEARERKDVEEALSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           L+NVKNQVN+MMEELQ++RN+GL+LENQIA SD MVKELE+KILSAIELLHNYKNDRD L
Sbjct: 363 LENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDVL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKEV+D+RTRQ +A  +H+AQ+FSEFSFSEIAEATRKFDPSLKIG+ THG +Y
Sbjct: 423 LKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV IKM  SHSLQNP+ F+REVDVL+ LRHPNI TLIG C E+C+LVYDYF N
Sbjct: 483 KGLLYNTEVCIKMFGSHSLQNPVEFQREVDVLSILRHPNIATLIGVCPEACVLVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           GSLEDRLACKDNSSPLSWKTRIRIA++LCSAL+F+H+NK+C++IH DL P N+LLD NY+
Sbjct: 543 GSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYV 602

Query: 601 PKLAGFGNCHFLPNDETTSVRADI------ESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG+CHFLP+DE  S   +I      +SNHE PLTTKLDVFSFG+VLL LLTGKS 
Sbjct: 603 PKLAGFGSCHFLPHDEKLSYNENIPAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSY 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
           + +K   +VQFA++ RKLK+VLDPRAGDWPFVQAEQLAQLA RCCN NSM RPDL SDVW
Sbjct: 663 LRMK--EDVQFAVEERKLKNVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPM+ASCG S+SV L  G+ Q+QPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGH+TSPMTNLRL+H+NLVPNRALRS IQEWLHQ
Sbjct: 783 LDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 813

BLAST of MS016951 vs. NCBI nr
Match: XP_022959086.1 (U-box domain-containing protein 33-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 610/816 (74.75%), Postives = 702/816 (86.03%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS G+ VVY+AVG D+ +C LNLVYAI+H GGRRIC++HVHEPAK+IP L T+F ASSMK
Sbjct: 3   GSCGDDVVYIAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPFLGTQFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           E+EV AYRE ERQDA+KILDGFL LCHQAG  AEKLYFES+K+KKGIVEL+SL GIRRLI
Sbjct: 63  EKEVNAYRERERQDANKILDGFLFLCHQAGVWAEKLYFESDKIKKGIVELVSLHGIRRLI 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRSRKA YVHL APAFCQIQFVC GQL   REACP+E+H D+P  
Sbjct: 123 MGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDVPPP 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ N+N A  RP++S QFNGSRI SPS    RLSIS  +S+ SGT SPFE  YS SS 
Sbjct: 183 SPQSQNVNGACWRPMESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGC+D ASSRTEEDEYG+GL+SPL L D+APN+SP  LS   GFQQD S DD LYIQLE+
Sbjct: 243 SGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSSPLHLS---GFQQDESADDILYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           +IT+ ANA+++AFREA++R +AE EL +AI R K  E LYAGE+RERK++E ALSKER E
Sbjct: 303 SITEAANAKREAFREAVKRAQAENELENAIFRAKFYEDLYAGEARERKDVEEALSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           L+NVKNQVN+MMEELQ++RN+GL+LENQIA SD MVKELE+KILSAIELLHNYKNDRDEL
Sbjct: 363 LENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKEV+D+RTRQ +A  +H+AQ+FSEFSFSEIAEATRKFDPSLKIG+ THG +Y
Sbjct: 423 LKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV IKM  SH+LQNP+ F+REVDVL+ LRHPNI TLIG C E+CILVYDYF N
Sbjct: 483 KGLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRHPNIATLIGVCPEACILVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           GSLEDRLACKDNSSPLSWKTRIRIA++LCSAL+F+H+NK+C++IH DL P N+LLD NY+
Sbjct: 543 GSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYV 602

Query: 601 PKLAGFGNCHFLPND------ETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG+CHFLP+D      E  S +  ++SN E PLTTKLDVFSFG+VLL LLTGKS 
Sbjct: 603 PKLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSY 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
           + +K   +VQFAI+ RKLKDVLDPRAGDWPFVQAEQLAQLA RCCN NSM RPDL SDVW
Sbjct: 663 LRMK--EDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPM+ASCG S+SV L  G+ Q+QPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGH+TSPMTNLRL+H+NLVPNRALRS IQEWLHQ
Sbjct: 783 LDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 813

BLAST of MS016951 vs. NCBI nr
Match: KAG7013740.1 (U-box domain-containing protein 33 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 609/816 (74.63%), Postives = 701/816 (85.91%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS G+ VVY+AVG D+ +C LNLVYAI+H GGRRIC++HVHEPAK+IP L T+F ASSMK
Sbjct: 3   GSCGDDVVYMAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPFLGTQFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           E+EV AYRE ERQDA+ ILDGFL LCHQAG  AEKLYFES+K+KKGIVEL+SL GIRRLI
Sbjct: 63  EKEVNAYRERERQDANNILDGFLFLCHQAGVWAEKLYFESDKIKKGIVELVSLHGIRRLI 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRSRKA YVHL APAFCQIQFVC GQL   REACP+E+H D+P  
Sbjct: 123 MGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDVPPP 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ N+N A  RP++S QFNGSRI SPS    RLSIS  +S+ SGT SPFE  YS SS 
Sbjct: 183 SPQSQNVNGACWRPMESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGC+D ASSRTEEDEYG+GL+SPL L D+APN+SP  LS   GFQQD S DD LYIQLE+
Sbjct: 243 SGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSSPLHLS---GFQQDESADDILYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           +IT+ ANA+++AFREA++R +AE EL +AI R K  E LYAGE+RERK++E ALSKER E
Sbjct: 303 SITEAANAKREAFREAVKRAQAENELGNAIFRAKFYEDLYAGEARERKDVEEALSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           L+NVKNQVN+MMEELQ++RN+GL+LENQIA SD MVKELE+KILSAIELLHNYKNDRDEL
Sbjct: 363 LENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKEV+D+RTRQ +A  +H+AQ+FSEFSFSEIAEATRKFDPSLKIG+ THG +Y
Sbjct: 423 LKQRDEALKEVDDIRTRQVEARSKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV IKM  SH+LQNP+ F+REVDVL+ LRHPNI TLIG C E+CILVYDYF N
Sbjct: 483 KGLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRHPNIATLIGVCPEACILVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           GSLEDRLACKDNSSPLSWKTRIRIA++LCSAL+F+H+NK+C++IH DL P N+LLD NY+
Sbjct: 543 GSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYV 602

Query: 601 PKLAGFGNCHFLPND------ETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG+CHFLP+D      E  S +  ++SN E PLTTKLDVFSFG+VLL LLTGKS 
Sbjct: 603 PKLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSY 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
           + +K   +VQFAI+ RKLKDVLDPRAGDWPFVQAEQLAQLA RCCN NSM RPDL SDVW
Sbjct: 663 LRMK--EDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPM+ASCG S+SV L  G+ Q+QPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGH+TSPMTNLRL+H+NLVPNRALRS IQEWLHQ
Sbjct: 783 LDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 813

BLAST of MS016951 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 734.9 bits (1896), Expect = 9.4e-211
Identity = 402/819 (49.08%), Postives = 545/819 (66.54%), Query Frame = 0

Query: 8   VYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEVKAY 67
           ++VAV K +   +  L++A+Q++GG++IC++HVH+P++MIP++  KFP  ++KEEEV+ +
Sbjct: 47  IFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEVRVF 106

Query: 68  RELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAAADK 127
           RE ER+  H ILD +L +C Q G RAEK++ E E ++ GIV+LIS LGIR+L+MGAAAD+
Sbjct: 107 REKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAAADR 166

Query: 128 HYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIRE----------ACPRESHADI 187
           HYSRRMT ++SRKA +V   AP  CQI F CKG L   RE          A PR S +  
Sbjct: 167 HYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSISAS 226

Query: 188 PLLSPQTHNINDASRRPVQSAQFNGS---RIDSPSRLSISNSMSDASGTWSPFEHLYSTS 247
            LL  QT +  ++  + +   Q   S    + + S    S  +SD S      E     S
Sbjct: 227 DLL--QTFSTPESEHQHISRVQSTDSVQQLVSNGSSTEQSGRVSDGSLNTDEEERESDGS 286

Query: 248 SYSGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQL 307
             +G   V SS         G SSP         +S PD            VDDS  +++
Sbjct: 287 EVTGSATVMSS---------GHSSP---------SSFPD-----------GVDDSFNVKI 346

Query: 308 EQAITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKER 367
            +A ++  +++++AF E +RR KAEK   DAI R K SE+ Y+ E + RK+ E A++KE+
Sbjct: 347 RKATSEAHSSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEK 406

Query: 368 GELDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRD 427
                +KN+   +MEELQ A  +   LE+QIA+SD  +++L +K+  A++LL   +++R+
Sbjct: 407 ERFITIKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDERE 466

Query: 428 ELLRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGI 487
           EL  +RD AL+E E++R+  E +T +   Q F++FSFSEI EAT  FD +LKIG+  +G 
Sbjct: 467 ELQTERDRALREAEELRSHAETSTLQ-LPQYFTDFSFSEIEEATNHFDSTLKIGEGGYGS 526

Query: 488 IYKGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYF 547
           IY GLL +T+VAIKML  +S Q P+ +++EVDVL+K+RHPNI+TLIGAC E   LVY+Y 
Sbjct: 527 IYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEYL 586

Query: 548 ANGSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGN 607
             GSLEDRL CKDNS PLSW+ R+RIAT++C+ALVFLH+NK   ++H DL PANILLD N
Sbjct: 587 PGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPANILLDSN 646

Query: 608 YIPKLAGFGNCHFLPNDETTSVRADI---------ESNHESPLTTKLDVFSFGIVLLRLL 667
            + KL+ FG C  L  + + SVR D+         E++    LT K DV+SFGI+LLRLL
Sbjct: 647 LVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGIILLRLL 706

Query: 668 TGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDL 727
           TG+    +++ +EV++A+D   L D+LDP AGDWPFVQAEQLA+LA RCC T S  RPDL
Sbjct: 707 TGRP--ALRISNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLALRCCETVSENRPDL 766

Query: 728 ASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAE 787
            ++VWRVLEPMRAS G S S  LG  E ++  PPYFICPIFQEVM+DPHVAADGFTYEAE
Sbjct: 767 GTEVWRVLEPMRASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQDPHVAADGFTYEAE 826

Query: 788 ALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL 805
           A+R WLDS HDTSPMTN++L H +L+ N ALRSAIQEWL
Sbjct: 827 AIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEWL 830

BLAST of MS016951 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.3e-135
Identity = 289/806 (35.86%), Postives = 465/806 (57.69%), Query Frame = 0

Query: 5   NHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEV 64
           +  ++VAV +D++  +  +++A ++  G++IC+L+VH  A+       K    S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 65  KAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAA 124
           K    +E+    ++++ +L L  +   + +KL    + +++ IVELI+   I+ L+MGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 125 ADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLLSPQT 184
           +DKHYS +MT ++S+KA +V   AP  C I F+CKG L   R +   +   +   + P  
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRAS--NDDSNNRQTMPPLV 193

Query: 185 HNINDASRRPVQSAQFNGSRIDSPSRLSISNSMSDASGTWSPFEHLYSTSSYSGCMDVAS 244
              +D   R  +S +   S +    R   S    D        E               S
Sbjct: 194 QLDSDNETR--KSEKLESSYMRRRLRYWRSLLEQDGEKDTGQLEREKVEPRAPPLFSSGS 253

Query: 245 SRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQAITDVANA 304
           S +  +  G    SP  +     NTS  +       +++G V   ++ + ++A+ D+  +
Sbjct: 254 SSSFGEPVGPEPVSPELVDSDTLNTSNVEEK-----EREGDVARKVH-RYDKAMHDIGQS 313

Query: 305 RKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGELDNVKNQV 364
            +  + EA ++ + +    +A+ + K  E L   ES +RK +E  L KE+ E+  V  Q 
Sbjct: 314 DRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVIEQN 373

Query: 365 NEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELLRQRDEAL 424
           N  M+ELQ+ + + LKLE+Q+ +   + KE  +K  +A+ELL +++  RDE+    + A+
Sbjct: 374 NGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHENAV 433

Query: 425 KEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYKGLLYNTE 484
           KEV  +R   +  TGE S     ++SF EI EAT +FDPS K+G+  +G +YKG L + +
Sbjct: 434 KEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQHLQ 493

Query: 485 VAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLA 544
           VA+KML S+   N   F R V++L+++RHPN++TL+GAC ES  L+Y Y  NGSLED  +
Sbjct: 494 VAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFS 553

Query: 545 CKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGN 604
            ++N   LSW++RIRIA+++CSAL+FLH+N  C +IH +L P+ ILLD N + K+  +G 
Sbjct: 554 SENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGI 613

Query: 605 CHFLPNDETTSVRADIESNH--ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFA 664
              +P D        ++ ++     +T + D+++FGI+LL+LLT +   +  +L +V+ A
Sbjct: 614 SQLIPIDGLDKSDPHVDPHYFVSREMTLESDIYAFGIILLQLLTRRP--VSGILRDVKCA 673

Query: 665 IDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGV 724
           ++   +  VLD  AGDWP  + ++LA +A RCC  N M RPDLA  V R ++ M+A   V
Sbjct: 674 LENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDLAV-VLRFIDRMKAP-EV 733

Query: 725 SLSVCLGSGEQQL--QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPM 784
             S       Q +  +PP +++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHDTSPM
Sbjct: 734 PSSETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPM 793

Query: 785 TNLRLDHRNLVPNRALRSAIQEWLHQ 807
           TNL+++  NL+PN AL  AIQ+W +Q
Sbjct: 794 TNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of MS016951 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.3e-111
Identity = 232/537 (43.20%), Postives = 327/537 (60.89%), Query Frame = 0

Query: 283 DGSVDDSLYIQLEQAITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRE 342
           D  ++D L  +L++++ + A  +K+A  E ++R ++E+         +  E LY  + ++
Sbjct: 288 DLHLNDDLCNKLQKSMDEAAVLKKEASDERLKRIESER-------LARTLEDLYLSQVQQ 347

Query: 343 RKEIEAALSKERGELDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSA 402
           RKE E +LS+ + E + +K Q +E+  ELQ    +   L  Q+++S    +E  + +LS 
Sbjct: 348 RKETEESLSRVQQEFEQLKIQQDEVTVELQRVNEQNENLLGQLSDS----REHFEWLLS- 407

Query: 403 IELLHNYKNDRDELLRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFD 462
                    + D+LLR+RD A++EVE++R ++          +  EFS SE+  AT  F 
Sbjct: 408 ---------EHDQLLRERDNAVREVEELRQKRGQMLSVLVTAMHCEFSSSEVESATENFS 467

Query: 463 PSLKIGDSTHGIIYKGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGA 522
            SLKIG+   G +YKG+L N  VAIK+LR  SLQ    F +EV +L+++RHP+++TL+GA
Sbjct: 468 NSLKIGEGGFGCVYKGILRNMTVAIKVLRPDSLQGQSQFEQEVSILSRVRHPHLVTLLGA 527

Query: 523 CSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHC 582
           CSES  LVY++  NGSLED L C D    L+W+ RIRI  ++CSAL+FLH NK   V+H 
Sbjct: 528 CSESSTLVYEFLPNGSLEDFLMCSDKRQTLTWQARIRIIAEICSALIFLHKNKPHPVVHG 587

Query: 583 DLNPANILLDGNYIPKLAGFGNCHFLPNDET-------------TSVRADIESNHESPLT 642
           DL PANILL  N + KL+ FG    L    T             T +  D E      LT
Sbjct: 588 DLKPANILLGVNLVSKLSDFGISRLLIQSSTNNTTLYRTMHPVGTPLYMDPEFLSTGELT 647

Query: 643 TKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQ 702
            + DV+SFGIV+LRLLTGK  + IK +  V+ A++   L  V+D   G+WP +  EQLA 
Sbjct: 648 PQSDVYSFGIVVLRLLTGKPPVGIKNI--VEDAMEKGDLNSVIDTSVGEWPHLHIEQLAY 707

Query: 703 LAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEV 762
           LA RC   +   RPDL+ +VW ++E +R    +S      S + Q  PP YFICPI Q++
Sbjct: 708 LALRCTELSRRCRPDLSGEVWAIVEAIR-DAALSSPSSSRSAQDQNSPPSYFICPISQDI 767

Query: 763 MEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           M+DPH+AADGFTYEAEA+R WL +GHDTSPMTNL L+H  L+PNRALRSAIQEWL Q
Sbjct: 768 MDDPHIAADGFTYEAEAIRSWLCNGHDTSPMTNLLLEHEELIPNRALRSAIQEWLQQ 800

BLAST of MS016951 vs. ExPASy Swiss-Prot
Match: Q0DR28 (U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB57 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 4.3e-86
Identity = 169/400 (42.25%), Postives = 238/400 (59.50%), Query Frame = 0

Query: 414 DELLRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHG 473
           D+L  +RD A+++V ++R        + S  +  +FS  ++ +AT  F  + ++GD+ +G
Sbjct: 120 DQLKHERDNAVQQVNELR--------DQSTHIILDFSRKDMEQATEHFKNAREVGDTEYG 179

Query: 474 IIYKGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDY 533
             YKG+++N +V IK+  S  L     F++EV +L + RHPNI+T IG CSE   LVY++
Sbjct: 180 HTYKGMIHNMKVLIKLSSSQKL-----FQQEVSILRQWRHPNIITFIGVCSEVSALVYEW 239

Query: 534 FANGSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDG 593
             NG+LEDR+ C +NS+PLSW  R +I  ++C AL+FLH+NK   ++H DL P NIL+D 
Sbjct: 240 LPNGNLEDRIICTNNSAPLSWYNRTQIIGEICCALLFLHSNKSTALVHGDLRPCNILIDA 299

Query: 594 NYIPKLAGFGNCH-------FLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLT 653
           NY  K+  FG  +       F PN        D E N    LTT  DV+S G+++LRLLT
Sbjct: 300 NYRSKICNFGMSNLFLQLGTFPPNLTARLPYMDPEFNTTGELTTLSDVYSLGVIILRLLT 359

Query: 654 GKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLA 713
           G     + L  +V  A+    L  ++D  AGDWP+++A+QLA +   C      KRPDL 
Sbjct: 360 GMPP--LTLSEKVAEALGSDSLHLLIDKSAGDWPYIEAKQLALIGLSCTGMTRKKRPDLL 419

Query: 714 SDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEA 773
           ++VW V+EP+      +    L S       P  FICPI  E+M+DP VA+DGFTYEAEA
Sbjct: 420 NEVWIVIEPLTRKPPAATWPYLQSASGDSSVPAAFICPISMEIMKDPQVASDGFTYEAEA 479

Query: 774 LRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           +R W D G   SPMTNL L + NLVPNR LRS I  +L Q
Sbjct: 480 IRCWFDRGISRSPMTNLALPNLNLVPNRVLRSFIHGYLQQ 504

BLAST of MS016951 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 300.8 bits (769), Expect = 4.6e-80
Identity = 238/813 (29.27%), Postives = 392/813 (48.22%), Query Frame = 0

Query: 37  ILHVHEPAKMIPI-LRTKFPASSMKEEEVKAYRELERQDAHKILDGFLLLCHQAGARAEK 96
           +LH+H     +P  +    P S ++++ V AYR+     + ++L  +  L  +     E 
Sbjct: 53  LLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEV 112

Query: 97  LYFESEKVKKGIVELISLLGIRRLIMGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQ 156
           L  ES+ V   I E ++   I R+++G ++   +SR     ++   + +    P FC + 
Sbjct: 113 LVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSR-----KADICSVISALMPNFCTVY 172

Query: 157 FVCKGQLTCIREACPRESHADIPLLSPQTHNINDASRRPVQSAQFNGSRIDSPSRLSISN 216
            V KG+L+C+R   P +S  +  +         D S R   S+  +G   DS   +S ++
Sbjct: 173 VVSKGKLSCVR---PSDSDGNATIR-------EDGSERTNSSSGSSGPTSDSSDVMSSAH 232

Query: 217 SMSDASGTWSPFEHLYSTSSYSG----CMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSP 276
             S +     P   +    + +G     M+ +S  ++E       +   R +     +S 
Sbjct: 233 D-SQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEARDVSSINRSST 292

Query: 277 PDLSVWNGFQQDGSVDDSLYIQLEQAITDVANARK----------QAFREAMRRGKAEKE 336
              S W   ++D       Y + ++A++  ++ R+                    +A ++
Sbjct: 293 DTTSRWTPRRRD-------YEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHSRASQQ 352

Query: 337 LADAIDRVKDSEALYAGESRERKEIEAALSKERGELDNVKNQVNEMMEELQIARNK---- 396
            ++  D +  SE  Y   +  +  +   + K R EL +V+        E   A  K    
Sbjct: 353 ASNMSDAL--SEQSY---TDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGEL 412

Query: 397 -GLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRD----------ELLRQRDEALKE 456
              +LE  I   +L +KE E + L+  E  +  K  RD          E+ ++R+   K 
Sbjct: 413 NQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKS 472

Query: 457 VEDMRTRQEDATGEHSAQL-FSEFSFSEIAEATRKFDPSLKIGDSTHGIIYKGLLYNTEV 516
             D + +++      S QL +  F++ EI  AT  F   LKIG   +G +YK  L++T  
Sbjct: 473 ARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTA 532

Query: 517 AIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLAC 576
            +K+L+S   Q    F++E+++L+K+RHP+++ L+GAC E   LVY+Y  NGSLEDRL  
Sbjct: 533 VVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQ 592

Query: 577 KDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFG-- 636
            +NS PL W  R RIA ++ +ALVFLH +K   +IH DL PANILLD N++ K+   G  
Sbjct: 593 VNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLS 652

Query: 637 --------NCHFLPNDETTSVRA----DIESNHESPLTTKLDVFSFGIVLLRLLTGKSDM 696
                   +  F    +T+ V      D E      +++K D++SFG++LL+LLT K   
Sbjct: 653 TMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKP-- 712

Query: 697 MIKLLHEVQFAIDGR-KLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 756
            I L H V+ A+D   +   +LD +AG+WP  +  +LA LA  C       RPDL   + 
Sbjct: 713 AIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQIL 772

Query: 757 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 804
             LE ++     + +   G      QPP +FICP+ ++VM +P VAADG+TY+  A+  W
Sbjct: 773 PALENLKKVAEKARNSFSG---VSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEW 831

BLAST of MS016951 vs. ExPASy TrEMBL
Match: A0A6J1DV42 (U-box domain-containing protein 33-like OS=Momordica charantia OX=3673 GN=LOC111024334 PE=4 SV=1)

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 722/810 (89.14%), Postives = 750/810 (92.59%), Query Frame = 0

Query: 7   VVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEVKA 66
           VVYVAVGKDIK+C+LNLVYA Q+SGGRRICILH+HEP KMI I   K PASSMKEEEV A
Sbjct: 16  VVYVAVGKDIKECKLNLVYAAQNSGGRRICILHIHEPPKMIRISGKKVPASSMKEEEVNA 75

Query: 67  YRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAAAD 126
            RELERQD HKILDGFLLLC  AGARAEKLYFES+K+K+G+V+LISL GIR+L+MGAAAD
Sbjct: 76  CRELERQDIHKILDGFLLLCRVAGARAEKLYFESDKIKEGLVKLISLCGIRKLVMGAAAD 135

Query: 127 KHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADI----PLLSP 186
           KHYS RM KIRSRKA YVHL APAFCQIQFVCKG L  IREACPRE+HA+I    PL  P
Sbjct: 136 KHYSTRMKKIRSRKAMYVHLKAPAFCQIQFVCKGMLIRIREACPREAHANIPLPLPLPLP 195

Query: 187 QTHN-INDASRRPVQSAQFNGSRIDSPSRL----SISNSMSDASGTWSPFEHLYSTSSYS 246
           QT N IN AS R VQS Q NGSR D+PS +    SISNSM DA G+WSPFEH YSTSS S
Sbjct: 196 QTQNIINGASWRSVQSGQSNGSRGDTPSIVLETRSISNSMLDA-GSWSPFEHPYSTSSCS 255

Query: 247 GCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQA 306
           GC++V SSRTEEDEYGLGL+ PL++ DL PN   P+L    GF+QD SVDDSLYI+LEQA
Sbjct: 256 GCINVPSSRTEEDEYGLGLNLPLQMKDLTPNLYSPNLL---GFEQDESVDDSLYIRLEQA 315

Query: 307 ITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGEL 366
           ITD ANA+++AF+EA RR KAEKEL DAI RVKDSEALYAGESRERKEIEAALSKERGEL
Sbjct: 316 ITDAANAKREAFKEATRRIKAEKELGDAIRRVKDSEALYAGESRERKEIEAALSKERGEL 375

Query: 367 DNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELL 426
           DNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELL
Sbjct: 376 DNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELL 435

Query: 427 RQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYK 486
           RQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYK
Sbjct: 436 RQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYK 495

Query: 487 GLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANG 546
           GLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANG
Sbjct: 496 GLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANG 555

Query: 547 SLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIP 606
           SLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIP
Sbjct: 556 SLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIP 615

Query: 607 KLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLH 666
           KLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLH
Sbjct: 616 KLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLH 675

Query: 667 EVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMR 726
           EVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMR
Sbjct: 676 EVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMR 735

Query: 727 ASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT 786
           ASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT
Sbjct: 736 ASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDT 795

Query: 787 SPMTNLRLDHRNLVPNRALRSAIQEWLHQP 808
           SPMTNLRLDHRNLVPNRALRSAIQEWLHQP
Sbjct: 796 SPMTNLRLDHRNLVPNRALRSAIQEWLHQP 821

BLAST of MS016951 vs. ExPASy TrEMBL
Match: A0A6J1H3V9 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 610/816 (74.75%), Postives = 702/816 (86.03%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS G+ VVY+AVG D+ +C LNLVYAI+H GGRRIC++HVHEPAK+IP L T+F ASSMK
Sbjct: 3   GSCGDDVVYIAVGNDVNECRLNLVYAIKHLGGRRICVVHVHEPAKLIPFLGTQFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           E+EV AYRE ERQDA+KILDGFL LCHQAG  AEKLYFES+K+KKGIVEL+SL GIRRLI
Sbjct: 63  EKEVNAYRERERQDANKILDGFLFLCHQAGVWAEKLYFESDKIKKGIVELVSLHGIRRLI 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRSRKA YVHL APAFCQIQFVC GQL   REACP+E+H D+P  
Sbjct: 123 MGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDVPPP 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ N+N A  RP++S QFNGSRI SPS    RLSIS  +S+ SGT SPFE  YS SS 
Sbjct: 183 SPQSQNVNGACWRPMESGQFNGSRIQSPSIVLERLSISKLVSNDSGTSSPFERSYSPSSS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGC+D ASSRTEEDEYG+GL+SPL L D+APN+SP  LS   GFQQD S DD LYIQLE+
Sbjct: 243 SGCLDAASSRTEEDEYGVGLNSPLLLKDVAPNSSPLHLS---GFQQDESADDILYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           +IT+ ANA+++AFREA++R +AE EL +AI R K  E LYAGE+RERK++E ALSKER E
Sbjct: 303 SITEAANAKREAFREAVKRAQAENELENAIFRAKFYEDLYAGEARERKDVEEALSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           L+NVKNQVN+MMEELQ++RN+GL+LENQIA SD MVKELE+KILSAIELLHNYKNDRDEL
Sbjct: 363 LENVKNQVNKMMEELQVSRNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKEV+D+RTRQ +A  +H+AQ+FSEFSFSEIAEATRKFDPSLKIG+ THG +Y
Sbjct: 423 LKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV IKM  SH+LQNP+ F+REVDVL+ LRHPNI TLIG C E+CILVYDYF N
Sbjct: 483 KGLLYNTEVCIKMFGSHNLQNPVEFQREVDVLSILRHPNIATLIGVCPEACILVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           GSLEDRLACKDNSSPLSWKTRIRIA++LCSAL+F+H+NK+C++IH DL P N+LLD NY+
Sbjct: 543 GSLEDRLACKDNSSPLSWKTRIRIASELCSALIFIHSNKICKIIHGDLKPLNVLLDANYV 602

Query: 601 PKLAGFGNCHFLPND------ETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG+CHFLP+D      E  S +  ++SN E PLTTKLDVFSFG+VLL LLTGKS 
Sbjct: 603 PKLAGFGSCHFLPHDEKLSYNENISAKYGVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSY 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
           + +K   +VQFAI+ RKLKDVLDPRAGDWPFVQAEQLAQLA RCCN NSM RPDL SDVW
Sbjct: 663 LRMK--EDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPM+ASCG S+SV L  G+ Q+QPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGH+TSPMTNLRL+H+NLVPNRALRS IQEWLHQ
Sbjct: 783 LDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 813

BLAST of MS016951 vs. ExPASy TrEMBL
Match: A0A6J1L2C9 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111499216 PE=4 SV=1)

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 605/816 (74.14%), Postives = 696/816 (85.29%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS G+ VVY+AVG D+ +C+LNLVYA++HSGGRRICILHVHEPAK+IP L T+F ASSMK
Sbjct: 3   GSCGDDVVYMAVGNDVNECKLNLVYALKHSGGRRICILHVHEPAKLIPFLGTQFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           E+EV AYRE ERQDA+KILDGFL LCHQAG RAEKLYFES+K+KKGIVEL+SL GIRRLI
Sbjct: 63  EKEVNAYRERERQDANKILDGFLFLCHQAGVRAEKLYFESDKIKKGIVELVSLHGIRRLI 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRSRKA YVHL APAFCQIQFVC GQL   REACP+E+H DIP  
Sbjct: 123 MGAAADKYYSRKMTKIRSRKAMYVHLKAPAFCQIQFVCNGQLIRTREACPQEAHVDIPPP 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ N+N A  RPV+  QFNGSRI SPS    RLSIS  + + SGT SP E  YS SS 
Sbjct: 183 SPQSQNVNGACWRPVEPGQFNGSRIQSPSIVLERLSISKLVLNDSGTSSPSERSYSPSSS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGC+D A SRTEED YG+GL+SPL L D+APN+SP  L    GFQQD S DD LYIQLE+
Sbjct: 243 SGCLDAACSRTEEDAYGVGLNSPLLLKDVAPNSSPLHLL---GFQQDESADDILYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           +IT+ ANA+++AFREA++R +AE EL +AI R K  E LYAGE+RERK++E ALSKER E
Sbjct: 303 SITEAANAKREAFREAVKRAQAENELGNAIFRAKFYEDLYAGEARERKDVEEALSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
            +NVKNQVN+MMEELQ+++N+GL+LENQIA SD MVKELE+KILSAIELLHNYKNDRDEL
Sbjct: 363 FENVKNQVNKMMEELQVSQNRGLELENQIAGSDQMVKELEQKILSAIELLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKEV+D+RTRQ +A  +H+AQ+FSEFSFSEIAEATRKFDPSLKIG+ THG +Y
Sbjct: 423 LKQRDEALKEVDDIRTRQVEARTKHTAQVFSEFSFSEIAEATRKFDPSLKIGEGTHGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV IKM  SH+LQNP+ F+REVD L+ +RHPNI TLIG C E+CILVYDYF N
Sbjct: 483 KGLLYNTEVCIKMFGSHNLQNPVEFQREVDALSIMRHPNIATLIGVCPEACILVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           GSLEDRLACKDNSSPLSWKTRIRIA+ LCSAL+F+H+NK+C++IH DL P N+LLD NY+
Sbjct: 543 GSLEDRLACKDNSSPLSWKTRIRIASDLCSALIFIHSNKICKIIHGDLKPLNVLLDANYV 602

Query: 601 PKLAGFGNCHFLPND------ETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG+CH LP+D      E  S +  ++SNHE PLTTKLDVFSFG+VLL LLTGKS 
Sbjct: 603 PKLAGFGSCHLLPHDEKLSYNENISAKYGVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSY 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
           + +K   +VQFAI+ RKLKDVLDPRAGDWPFVQAEQLAQLA RCCN NSM RPDL SDVW
Sbjct: 663 LRMK--EDVQFAIEERKLKDVLDPRAGDWPFVQAEQLAQLALRCCNRNSMYRPDLVSDVW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPM+ASCG S+SV L  G+ Q+QPPPYF+CPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMKASCGGSVSVSLSFGDLQVQPPPYFLCPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGH+TSPMTNLRL+H+NLVPNRALRS IQEWLHQ
Sbjct: 783 LDSGHNTSPMTNLRLEHQNLVPNRALRSVIQEWLHQ 813

BLAST of MS016951 vs. ExPASy TrEMBL
Match: A0A0A0KD01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 617/816 (75.61%), Postives = 688/816 (84.31%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS+G  +VYVAVG DI +C+LNLVYAI+HSGGRRICILHVHEPAK IPIL TKF ASSMK
Sbjct: 3   GSSGEDLVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSMK 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           EEEV AYRELERQDA KILD FLLLCHQAGARAEKLYFES+K+KKGIVEL+SL GIR L+
Sbjct: 63  EEEVNAYRELERQDADKILDVFLLLCHQAGARAEKLYFESDKIKKGIVELVSLHGIRSLV 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRSRKA YVHLNA A CQIQFVCKGQL  IREACP+E+HADI   
Sbjct: 123 MGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQETHADISPS 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ NIN  S R  Q   FNG   +SP+    RL+IS ++S+ASG  SPFE  Y+TS  
Sbjct: 183 SPQSQNINGVSWRTEQFGLFNGRISNSPTIVMERLTISETISNASG--SPFERSYATSPS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SG MDVASSRTEED YGLGL+SPL L + A  TSPP  S   GFQQDGS DDSLYIQLE+
Sbjct: 243 SGYMDVASSRTEEDAYGLGLNSPLLLTNFATKTSPPHFS---GFQQDGSADDSLYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           AI D ANAR++AFREA++R KAEKEL +AI   K SE LYA ESR RK+ E  LSKER E
Sbjct: 303 AIADAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKDAEEELSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           LD+VKNQVNEMM+ELQIARN GLKLENQIA+SD MVKELE+KILSAIELLHNYKNDRDEL
Sbjct: 363 LDDVKNQVNEMMKELQIARNNGLKLENQIAQSDEMVKELEQKILSAIELLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEALKE++D+RTRQ +A  +HSAQL SEFSFSEI EATRKFDPSLKI    +G +Y
Sbjct: 423 LKQRDEALKELDDIRTRQVEAMSQHSAQLISEFSFSEIVEATRKFDPSLKIVTDANGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV+IKML SH+LQNP+ F+REVD L+KLRHPNI TLIG C E+CILVYDYF N
Sbjct: 483 KGLLYNTEVSIKMLCSHNLQNPVDFQREVDALSKLRHPNIATLIGVCPEACILVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           G+LEDRLACKDNSSPLSWKTRI IATQLCSAL+F+H+NK+C+ +H D+ P+NILLD NY+
Sbjct: 543 GNLEDRLACKDNSSPLSWKTRILIATQLCSALIFIHSNKICKTVHGDMKPSNILLDANYV 602

Query: 601 PKLAGFGNCHFLPND------ETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG CHFL +D      E  SV  D + NHE PLTT+LD++SFG VLL LLTGKS 
Sbjct: 603 PKLAGFGTCHFLTHDEKLSYNENLSVGYDAKRNHEFPLTTELDIYSFGTVLLSLLTGKSY 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
           + +K   +VQ AI  RKLKDVLDPRAGDWPFVQAEQLAQLA RCC+T+SM RPDL SDVW
Sbjct: 663 LRMK--EDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCDTDSMYRPDLVSDVW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPMRASCG SLSVCL  G+QQ+QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGHDTSPMTNLRLDH+NLVPNRALRS IQEWL Q
Sbjct: 783 LDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 811

BLAST of MS016951 vs. ExPASy TrEMBL
Match: A0A5D3BV84 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001100 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 609/816 (74.63%), Postives = 689/816 (84.44%), Query Frame = 0

Query: 1   GSTGNHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMK 60
           GS G   VYVAVG DI +C+LNLVYAI+HSGGRRICILHVHEPAK IPIL TKF ASSM+
Sbjct: 3   GSGGEDWVYVAVGNDINECKLNLVYAIKHSGGRRICILHVHEPAKFIPILGTKFLASSME 62

Query: 61  EEEVKAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLI 120
           EEEV AYRELERQDA+KILD FLLLCHQAGA+AEKLYFES+K+KKGIVEL+SL GIR L+
Sbjct: 63  EEEVNAYRELERQDANKILDVFLLLCHQAGAQAEKLYFESDKIKKGIVELVSLHGIRSLV 122

Query: 121 MGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLL 180
           MGAAADK+YSR+MTKIRSRKA YVHLNA A CQIQFVCKGQL  IREACP+E+HADI   
Sbjct: 123 MGAAADKYYSRKMTKIRSRKAMYVHLNAAASCQIQFVCKGQLIRIREACPQEAHADISPS 182

Query: 181 SPQTHNINDASRRPVQSAQFNGSRIDSPS----RLSISNSMSDASGTWSPFEHLYSTSSY 240
           SPQ+ NIN  S R  Q  QFNG   +SPS    RL+IS ++S+ASGTWSPFE  Y+TS  
Sbjct: 183 SPQSQNINGVSWRTDQFGQFNGRISNSPSIVMERLTISETISNASGTWSPFERSYATSPS 242

Query: 241 SGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQ 300
           SGC DVASSR+EED YGLGLSSPLRL + APNTSP      +GFQQDG+ +DSLYIQLE+
Sbjct: 243 SGCTDVASSRSEEDSYGLGLSSPLRLTNFAPNTSPH----LSGFQQDGNANDSLYIQLEK 302

Query: 301 AITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGE 360
           AITD ANAR++AFREA++R KAEKEL +AI   K SE LYA ESR RKE E ALSKER E
Sbjct: 303 AITDAANARREAFREALKRAKAEKELDEAICWAKVSETLYAEESRGRKEAEEALSKEREE 362

Query: 361 LDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDEL 420
           LDNVKNQVNEMM++LQIA+N  LKLENQIA+SD MVKELE+KILSAI+LLHNYKNDRDEL
Sbjct: 363 LDNVKNQVNEMMKDLQIAQNNELKLENQIAQSDEMVKELEQKILSAIQLLHNYKNDRDEL 422

Query: 421 LRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIY 480
           L+QRDEAL+E++D+R RQ +A  + SAQL SEFSFSEI EATRKFDPSLKI +  +G +Y
Sbjct: 423 LKQRDEALQELDDIRKRQVEARSQRSAQLISEFSFSEIVEATRKFDPSLKIVEGENGSMY 482

Query: 481 KGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFAN 540
           KGLLYNTEV+IKML SH+LQNP+ F+REVD L+KLRHPN+ TLIG C E+CILVYDYF N
Sbjct: 483 KGLLYNTEVSIKMLCSHNLQNPVEFQREVDALSKLRHPNLATLIGVCPEACILVYDYFPN 542

Query: 541 GSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYI 600
           G+LEDRLACKDNSSPLSWKTRI IAT LCSAL+F+H+NK+C+++H D+ P+N+LLD NY+
Sbjct: 543 GNLEDRLACKDNSSPLSWKTRILIATDLCSALIFIHSNKICKIVHGDMKPSNVLLDANYV 602

Query: 601 PKLAGFGNCHFLPNDETT------SVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSD 660
           PKLAGFG CHFL +DE +      SV  D   NHE PLTT  DV+SFG+VLL LLTG+S 
Sbjct: 603 PKLAGFGICHFLTHDEKSSYNENLSVGHDAMGNHEFPLTTAFDVYSFGMVLLSLLTGQS- 662

Query: 661 MMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 720
             +++  +VQ AI  RKLKDVLDPRAGDWPFVQAEQLAQLA RCCNT+S  RPDL SD W
Sbjct: 663 -YLRMEEDVQIAIKKRKLKDVLDPRAGDWPFVQAEQLAQLALRCCNTDSTYRPDLVSDAW 722

Query: 721 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 780
           RVLEPMRASCG SLSVCL  G+QQ+QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW
Sbjct: 723 RVLEPMRASCGGSLSVCLSFGDQQVQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 782

Query: 781 LDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           LDSGHDTSPMTNLRLDH+NLVPNRALRS IQEWL Q
Sbjct: 783 LDSGHDTSPMTNLRLDHQNLVPNRALRSVIQEWLQQ 812

BLAST of MS016951 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 734.9 bits (1896), Expect = 6.7e-212
Identity = 402/819 (49.08%), Postives = 545/819 (66.54%), Query Frame = 0

Query: 8   VYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEVKAY 67
           ++VAV K +   +  L++A+Q++GG++IC++HVH+P++MIP++  KFP  ++KEEEV+ +
Sbjct: 47  IFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEVRVF 106

Query: 68  RELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAAADK 127
           RE ER+  H ILD +L +C Q G RAEK++ E E ++ GIV+LIS LGIR+L+MGAAAD+
Sbjct: 107 REKEREKVHMILDDYLRICQQRGVRAEKMFIEMESIENGIVQLISELGIRKLVMGAAADR 166

Query: 128 HYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIRE----------ACPRESHADI 187
           HYSRRMT ++SRKA +V   AP  CQI F CKG L   RE          A PR S +  
Sbjct: 167 HYSRRMTDLKSRKAIFVRREAPTLCQIWFTCKGYLIHTREATMDDTESEYASPRPSISAS 226

Query: 188 PLLSPQTHNINDASRRPVQSAQFNGS---RIDSPSRLSISNSMSDASGTWSPFEHLYSTS 247
            LL  QT +  ++  + +   Q   S    + + S    S  +SD S      E     S
Sbjct: 227 DLL--QTFSTPESEHQHISRVQSTDSVQQLVSNGSSTEQSGRVSDGSLNTDEEERESDGS 286

Query: 248 SYSGCMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQL 307
             +G   V SS         G SSP         +S PD            VDDS  +++
Sbjct: 287 EVTGSATVMSS---------GHSSP---------SSFPD-----------GVDDSFNVKI 346

Query: 308 EQAITDVANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKER 367
            +A ++  +++++AF E +RR KAEK   DAI R K SE+ Y+ E + RK+ E A++KE+
Sbjct: 347 RKATSEAHSSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEK 406

Query: 368 GELDNVKNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRD 427
                +KN+   +MEELQ A  +   LE+QIA+SD  +++L +K+  A++LL   +++R+
Sbjct: 407 ERFITIKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDERE 466

Query: 428 ELLRQRDEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGI 487
           EL  +RD AL+E E++R+  E +T +   Q F++FSFSEI EAT  FD +LKIG+  +G 
Sbjct: 467 ELQTERDRALREAEELRSHAETSTLQ-LPQYFTDFSFSEIEEATNHFDSTLKIGEGGYGS 526

Query: 488 IYKGLLYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYF 547
           IY GLL +T+VAIKML  +S Q P+ +++EVDVL+K+RHPNI+TLIGAC E   LVY+Y 
Sbjct: 527 IYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEYL 586

Query: 548 ANGSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGN 607
             GSLEDRL CKDNS PLSW+ R+RIAT++C+ALVFLH+NK   ++H DL PANILLD N
Sbjct: 587 PGGSLEDRLTCKDNSPPLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPANILLDSN 646

Query: 608 YIPKLAGFGNCHFLPNDETTSVRADI---------ESNHESPLTTKLDVFSFGIVLLRLL 667
            + KL+ FG C  L  + + SVR D+         E++    LT K DV+SFGI+LLRLL
Sbjct: 647 LVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGIILLRLL 706

Query: 668 TGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDL 727
           TG+    +++ +EV++A+D   L D+LDP AGDWPFVQAEQLA+LA RCC T S  RPDL
Sbjct: 707 TGRP--ALRISNEVKYALDNGTLNDLLDPLAGDWPFVQAEQLARLALRCCETVSENRPDL 766

Query: 728 ASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAE 787
            ++VWRVLEPMRAS G S S  LG  E ++  PPYFICPIFQEVM+DPHVAADGFTYEAE
Sbjct: 767 GTEVWRVLEPMRASSGGSSSFHLGRNEHRI-APPYFICPIFQEVMQDPHVAADGFTYEAE 826

Query: 788 ALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL 805
           A+R WLDS HDTSPMTN++L H +L+ N ALRSAIQEWL
Sbjct: 827 AIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEWL 830

BLAST of MS016951 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 9.3e-137
Identity = 289/806 (35.86%), Postives = 465/806 (57.69%), Query Frame = 0

Query: 5   NHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEV 64
           +  ++VAV +D++  +  +++A ++  G++IC+L+VH  A+       K    S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 65  KAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAA 124
           K    +E+    ++++ +L L  +   + +KL    + +++ IVELI+   I+ L+MGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 125 ADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADIPLLSPQT 184
           +DKHYS +MT ++S+KA +V   AP  C I F+CKG L   R +   +   +   + P  
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRAS--NDDSNNRQTMPPLV 193

Query: 185 HNINDASRRPVQSAQFNGSRIDSPSRLSISNSMSDASGTWSPFEHLYSTSSYSGCMDVAS 244
              +D   R  +S +   S +    R   S    D        E               S
Sbjct: 194 QLDSDNETR--KSEKLESSYMRRRLRYWRSLLEQDGEKDTGQLEREKVEPRAPPLFSSGS 253

Query: 245 SRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQAITDVANA 304
           S +  +  G    SP  +     NTS  +       +++G V   ++ + ++A+ D+  +
Sbjct: 254 SSSFGEPVGPEPVSPELVDSDTLNTSNVEEK-----EREGDVARKVH-RYDKAMHDIGQS 313

Query: 305 RKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGELDNVKNQV 364
            +  + EA ++ + +    +A+ + K  E L   ES +RK +E  L KE+ E+  V  Q 
Sbjct: 314 DRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMVIEQN 373

Query: 365 NEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELLRQRDEAL 424
           N  M+ELQ+ + + LKLE+Q+ +   + KE  +K  +A+ELL +++  RDE+    + A+
Sbjct: 374 NGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDHENAV 433

Query: 425 KEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYKGLLYNTE 484
           KEV  +R   +  TGE S     ++SF EI EAT +FDPS K+G+  +G +YKG L + +
Sbjct: 434 KEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNLQHLQ 493

Query: 485 VAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLA 544
           VA+KML S+   N   F R V++L+++RHPN++TL+GAC ES  L+Y Y  NGSLED  +
Sbjct: 494 VAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFS 553

Query: 545 CKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGN 604
            ++N   LSW++RIRIA+++CSAL+FLH+N  C +IH +L P+ ILLD N + K+  +G 
Sbjct: 554 SENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGI 613

Query: 605 CHFLPNDETTSVRADIESNH--ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFA 664
              +P D        ++ ++     +T + D+++FGI+LL+LLT +   +  +L +V+ A
Sbjct: 614 SQLIPIDGLDKSDPHVDPHYFVSREMTLESDIYAFGIILLQLLTRRP--VSGILRDVKCA 673

Query: 665 IDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGV 724
           ++   +  VLD  AGDWP  + ++LA +A RCC  N M RPDLA  V R ++ M+A   V
Sbjct: 674 LENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDLAV-VLRFIDRMKAP-EV 733

Query: 725 SLSVCLGSGEQQL--QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPM 784
             S       Q +  +PP +++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHDTSPM
Sbjct: 734 PSSETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPM 793

Query: 785 TNLRLDHRNLVPNRALRSAIQEWLHQ 807
           TNL+++  NL+PN AL  AIQ+W +Q
Sbjct: 794 TNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of MS016951 vs. TAIR 10
Match: AT3G49060.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 462.6 bits (1189), Expect = 6.4e-130
Identity = 281/810 (34.69%), Postives = 459/810 (56.67%), Query Frame = 0

Query: 5   NHVVYVAVGKDIKDCELNLVYAIQHSGGRRICILHVHEPAKMIPILRTKFPASSMKEEEV 64
           +  ++VAV +D++  +  +++A ++  G++IC+L+VH  A+       K    S K+ +V
Sbjct: 14  DETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDV 73

Query: 65  KAYRELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAA 124
           K    +E+    ++++ +L L  +   + +KL    + +++ IVELI+   I+ L+MGAA
Sbjct: 74  KVIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAA 133

Query: 125 ADKHYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREACPRESHADI--PLLSP 184
           +DKHYS +MT ++S+KA +V   AP  C I F+CKG L   R +    ++     PL+  
Sbjct: 134 SDKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIFTRASNDDSNNRQTMPPLVQL 193

Query: 185 QTHNINDASRRPVQSAQFNGSRI--DSPSRLSISNSMSDASGTWSPFEHLYSTSSYSGCM 244
            + N    S +   S      R       ++++  + +D        E            
Sbjct: 194 DSDNETRKSEKLESSYMRRRLRYWRSLLEQVNLLQNCADGEKDTGQLEREKVEPRAPPLF 253

Query: 245 DVASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQAITD 304
              SS +  +  G    SP  +     NTS  +       +++G V   ++ + ++A+ D
Sbjct: 254 SSGSSSSFGEPVGPEPVSPELVDSDTLNTSNVEEK-----EREGDVARKVH-RYDKAMHD 313

Query: 305 VANARKQAFREAMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGELDNV 364
           +  + +  + EA ++ + +    +A+ + K  E L   ES +RK +E  L KE+ E+  V
Sbjct: 314 IGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLEKEKLEVKMV 373

Query: 365 KNQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELLRQR 424
             Q N  M+ELQ+ + + LKLE+Q+ +   + KE  +K  +A+ELL +++  RDE+    
Sbjct: 374 IEQNNGFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMELLKSFRQKRDEIRIDH 433

Query: 425 DEALKEVEDMRTRQEDATGEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYKGLL 484
           + A+KEV  +R   +  TGE S     ++SF EI EAT +FDPS K+G+  +G +YKG L
Sbjct: 434 ENAVKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDPSWKLGEGKYGSVYKGNL 493

Query: 485 YNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLE 544
            + +VA                  V++L+++RHPN++TL+GAC ES  L+Y Y  NGSLE
Sbjct: 494 QHLQVA------------------VEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLE 553

Query: 545 DRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLA 604
           D  + ++N   LSW++RIRIA+++CSAL+FLH+N  C +IH +L P+ ILLD N + K+ 
Sbjct: 554 DCFSSENNVPALSWESRIRIASEICSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKIN 613

Query: 605 GFGNCHFLPNDETTSVRADIESNH--ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHE 664
            +G    +P D        ++ ++     +T + D+++FGI+LL+LLT +   +  +L +
Sbjct: 614 DYGISQLIPIDGLDKSDPHVDPHYFVSREMTLESDIYAFGIILLQLLTRRP--VSGILRD 673

Query: 665 VQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRA 724
           V+ A++   +  VLD  AGDWP  + ++LA +A RCC  N M RPDLA  V R ++ M+A
Sbjct: 674 VKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKNPMNRPDLAV-VLRFIDRMKA 733

Query: 725 SCGVSLSVCLGSGEQQL--QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHD 784
              V  S       Q +  +PP +++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHD
Sbjct: 734 P-EVPSSETSSYANQNVPRRPPSHYLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHD 793

Query: 785 TSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           TSPMTNL+++  NL+PN AL  AIQ+W +Q
Sbjct: 794 TSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 794

BLAST of MS016951 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 300.8 bits (769), Expect = 3.2e-81
Identity = 238/813 (29.27%), Postives = 392/813 (48.22%), Query Frame = 0

Query: 37  ILHVHEPAKMIPI-LRTKFPASSMKEEEVKAYRELERQDAHKILDGFLLLCHQAGARAEK 96
           +LH+H     +P  +    P S ++++ V AYR+     + ++L  +  L  +     E 
Sbjct: 53  LLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEV 112

Query: 97  LYFESEKVKKGIVELISLLGIRRLIMGAAADKHYSRRMTKIRSRKATYVHLNAPAFCQIQ 156
           L  ES+ V   I E ++   I R+++G ++   +SR     ++   + +    P FC + 
Sbjct: 113 LVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSFFSR-----KADICSVISALMPNFCTVY 172

Query: 157 FVCKGQLTCIREACPRESHADIPLLSPQTHNINDASRRPVQSAQFNGSRIDSPSRLSISN 216
            V KG+L+C+R   P +S  +  +         D S R   S+  +G   DS   +S ++
Sbjct: 173 VVSKGKLSCVR---PSDSDGNATIR-------EDGSERTNSSSGSSGPTSDSSDVMSSAH 232

Query: 217 SMSDASGTWSPFEHLYSTSSYSG----CMDVASSRTEEDEYGLGLSSPLRLIDLAPNTSP 276
             S +     P   +    + +G     M+ +S  ++E       +   R +     +S 
Sbjct: 233 D-SQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEARDVSSINRSST 292

Query: 277 PDLSVWNGFQQDGSVDDSLYIQLEQAITDVANARK----------QAFREAMRRGKAEKE 336
              S W   ++D       Y + ++A++  ++ R+                    +A ++
Sbjct: 293 DTTSRWTPRRRD-------YEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHSRASQQ 352

Query: 337 LADAIDRVKDSEALYAGESRERKEIEAALSKERGELDNVKNQVNEMMEELQIARNK---- 396
            ++  D +  SE  Y   +  +  +   + K R EL +V+        E   A  K    
Sbjct: 353 ASNMSDAL--SEQSY---TDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGEL 412

Query: 397 -GLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRD----------ELLRQRDEALKE 456
              +LE  I   +L +KE E + L+  E  +  K  RD          E+ ++R+   K 
Sbjct: 413 NQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKS 472

Query: 457 VEDMRTRQEDATGEHSAQL-FSEFSFSEIAEATRKFDPSLKIGDSTHGIIYKGLLYNTEV 516
             D + +++      S QL +  F++ EI  AT  F   LKIG   +G +YK  L++T  
Sbjct: 473 ARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTA 532

Query: 517 AIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLAC 576
            +K+L+S   Q    F++E+++L+K+RHP+++ L+GAC E   LVY+Y  NGSLEDRL  
Sbjct: 533 VVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQ 592

Query: 577 KDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFG-- 636
            +NS PL W  R RIA ++ +ALVFLH +K   +IH DL PANILLD N++ K+   G  
Sbjct: 593 VNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLS 652

Query: 637 --------NCHFLPNDETTSVRA----DIESNHESPLTTKLDVFSFGIVLLRLLTGKSDM 696
                   +  F    +T+ V      D E      +++K D++SFG++LL+LLT K   
Sbjct: 653 TMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKP-- 712

Query: 697 MIKLLHEVQFAIDGR-KLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVW 756
            I L H V+ A+D   +   +LD +AG+WP  +  +LA LA  C       RPDL   + 
Sbjct: 713 AIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQIL 772

Query: 757 RVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGW 804
             LE ++     + +   G      QPP +FICP+ ++VM +P VAADG+TY+  A+  W
Sbjct: 773 PALENLKKVAEKARNSFSG---VSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEW 831

BLAST of MS016951 vs. TAIR 10
Match: AT5G61560.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 283.5 bits (724), Expect = 5.3e-76
Identity = 216/769 (28.09%), Postives = 371/769 (48.24%), Query Frame = 0

Query: 68  RELERQDAHKILDGFLLLCHQAGARAEKLYFESEKVKKGIVELISLLGIRRLIMGAAADK 127
           ++++R+    +L    +  H+   + + +  ES+ +   I + +   GI  L++GA++  
Sbjct: 65  KDVDRKTREMLLPSRDMFVHRE-VQLDIMVLESDDIADAISKAVQDHGISELVIGASSSI 124

Query: 128 HYSRRMTKIRSRKATYVHLNAPAFCQIQFVCKGQLTCIREA--CPRESHAD---IPLLSP 187
            +S ++   RS  ++ +    P FC +  + KG+L  +R++      S AD       S 
Sbjct: 125 IFSWKLK--RSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSS 184

Query: 188 QTHNINDASRRPVQSAQFNGSRIDSPSRLSISNSMSDASGTWSPFEHLYSTSSYSGCMDV 247
            +H+++  S     S      RI + + ++     +       P  H ++    +G +DV
Sbjct: 185 DSHSVSSTSSHQFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNR---AGSLDV 244

Query: 248 ASSRTEEDEYGLGLSSPLRLIDLAPNTSPPDLSVWNGFQQDGSVDDSLYIQLEQAITDVA 307
             S+       L      R           D+S W   Q + +   S Y       +D  
Sbjct: 245 DESKL------LNQKGFYRTSSSGIGYGGSDISSWRSSQMEEASSSSTY-------SDPT 304

Query: 308 NARKQAFRE-AMRRGKAEKELADAIDRVKDSEALYAGESRERKEIEAALSKERGELDNVK 367
           ++  Q  ++  + + K E      +  V  SE + A  S++ +++    S+E   L N+ 
Sbjct: 305 SSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDA--SKKMQDLNQRRSEEATRLKNLT 364

Query: 368 NQVNEMMEELQIARNKGLKLENQIAESDLMVKELEKKILSAIELLHNYKNDRDELLRQRD 427
            +  E  E +++ R +    EN   E++L+ + +E++    +E                +
Sbjct: 365 IREEEADEVVEMERERQEDAEN---EAELVRECIERETEERLE---------------AE 424

Query: 428 EALKEVEDMRTRQEDAT--GEHSAQLFSEFSFSEIAEATRKFDPSLKIGDSTHGIIYKGL 487
              +EV   + R EDA   G    Q + +F + EI EAT  F   LKIG   +G +Y+  
Sbjct: 425 ARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCN 484

Query: 488 LYNTEVAIKMLRSHSLQNPLAFRREVDVLNKLRHPNIMTLIGACSESCILVYDYFANGSL 547
           L++T VA+K+L S        F +E+++L+K+RHP+++ L+GAC E   LVY+Y  NGSL
Sbjct: 485 LHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSL 544

Query: 548 EDRLACK------DNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDG 607
           E+RL  +          PL W  R RIA ++ SAL FLHTN+   ++H DL PANILLD 
Sbjct: 545 EERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDR 604

Query: 608 NYIPKLAGFGNCHFLPNDET-------------TSVRADIESNHESPLTTKLDVFSFGIV 667
           N + K+   G    +  D +             T    D E      +T + D+++FGI+
Sbjct: 605 NNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGII 664

Query: 668 LLRLLTGKSDMMIKLLHEVQFAIDGR--KLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTN 727
           LL+L+T +S M   L H ++ A+  +  K  ++LD  AGDWP  +A+++  +  RC    
Sbjct: 665 LLQLVTARSAM--GLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMR 724

Query: 728 SMKRPDLASDVWRVLEPMRASCGVSLSVCLGS-GEQQLQPPPYFICPIFQEVMEDPHVAA 787
              RPDL  ++  VLE ++    ++ ++   +  +     P +F CPI ++VME+P VA+
Sbjct: 725 KRDRPDLGKEILPVLERLKEVASIARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVAS 784

Query: 788 DGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQ 807
           DG+TYE  A++ WL   H  SPMT+L     +L+PN +L SAI+EW  Q
Sbjct: 785 DGYTYEKRAIKEWLQKNH-KSPMTDLPFPSDSLLPNHSLLSAIKEWRSQ 791