MS016870 (gene) Bitter gourd (TR) v1

BLAST of MS016870 vs. NCBI nr
Match: XP_022146365.1 (putative B3 domain-containing protein At2g27410 [Momordica charantia])

HSP 1 Score: 241.9 bits (616), Expect = 6.0e-60
Identity = 152/280 (54.29%), Postives = 190/280 (67.86%), Query Frame = 0

Query: 1   MGMDMI---EGLLPELSFPDSTHGVCNIPRKRR---SSSSAEES-----KPEDEA----- 60
           MGM  +   E L   + F D T+G+ +IP++RR   SSSSAE S     KP   A     
Sbjct: 1   MGMGTVVELEALKMGICFSDLTNGI-SIPQRRRTGSSSSSAETSETNKPKPSSSATAPMS 60

Query: 61  MLEAARILMAMANG-----GFCNNEIELNNRKRKKPPLALTKKQI--RKKRRKEIKLPST 120
           M+EAA +L+AMA        FC +E+E N+RKRK+ P++ ++ ++  RKK+RK ++LP T
Sbjct: 61  MVEAAAVLLAMAYSATDVEAFCKSELEKNSRKRKRDPVSKSEPEMKQRKKKRKAMELPVT 120

Query: 121 PEMPAATRDRIAEIGGYGIQLVI----QHTDLNKNHGRLSLPTKKLRVY-FATEQERKLL 180
           PEMPAA RDRI EIGGYGIQLVI    Q+TD+NKNHGRLSLP KKLR++ F TE+ERKLL
Sbjct: 121 PEMPAAMRDRIVEIGGYGIQLVIQKQLQNTDMNKNHGRLSLPAKKLRIHDFVTEEERKLL 180

Query: 181 YEHEKKKKKKKKKKKKKKKGMEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEE 240
            +H              KKGM+V I+DP L+ER I LKKWKIGSGD YCLMT WNS VEE
Sbjct: 181 NQH---------SNNNNKKGMDVPIIDPDLKERRICLKKWKIGSGDLYCLMTQWNSFVEE 240

Query: 241 SALRSGDFIQLWCFRKNCFDSETQTIRV---RLCFAMLKL 250
           +ALRSGDFIQLW FRK+ FD   +T ++   RL  AM+KL
Sbjct: 241 TALRSGDFIQLWSFRKDEFDEAEETEKISTSRLWLAMVKL 270

BLAST of MS016870 vs. NCBI nr
Match: XP_038875653.1 (B3 domain-containing protein At1g05920-like [Benincasa hispida])

HSP 1 Score: 216.9 bits (551), Expect = 2.1e-52
Identity = 144/257 (56.03%), Postives = 166/257 (64.59%), Query Frame = 0

Query: 12  ELSFPDSTHGVCNIPRKRRSSSS-------AEESKPEDEAMLEAARILMAMANG-----G 71
           EL  PD  + +C IPR+R SS++       AE SKP   +MLEAA +LMA+AN       
Sbjct: 4   ELGLPD-LNKLC-IPRRRSSSAAGKKEQHLAESSKP-PISMLEAAVVLMAIANSVSDVES 63

Query: 72  FCNNEIEL--NNRKRKKPP-LALTKKQIRKKRRKEIKLPSTPEMPAATRDRIAEIGGYGI 131
           FCN E++   N  KRK PP  A TKK  RKKR ++ K PS PEMPAA RDRI E+GGY I
Sbjct: 64  FCNCELKKYENTHKRKNPPTTAATKKPPRKKRARQSKFPSIPEMPAAMRDRIVEMGGYEI 123

Query: 132 QLVI----QHTDLNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKKKKKKG 191
            LVI    Q TDLNKN GRLSLP KKL   FATE+ER+LL + E           K KKG
Sbjct: 124 NLVIQKQLQDTDLNKNLGRLSLPQKKLLFDFATEEERRLLSQQE----------NKNKKG 183

Query: 192 MEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFRKNCFD 250
           + VVIMD VL ER I LKKWKIGSGD YCLMT WN+ V+E  L+SGD IQLW FRK   D
Sbjct: 184 LNVVIMDHVLEERNICLKKWKIGSGDVYCLMTQWNAFVKERGLKSGDHIQLWSFRK---D 243

BLAST of MS016870 vs. NCBI nr
Match: XP_038876237.1 (putative B3 domain-containing protein At3g24850 [Benincasa hispida])

HSP 1 Score: 206.1 bits (523), Expect = 3.6e-49
Identity = 139/265 (52.45%), Postives = 160/265 (60.38%), Query Frame = 0

Query: 1   MGM-DMIEGLLPELSFPDSTHGVCNIPRKRRSSSSA---EESKPEDE---AMLEAARILM 60
           MGM   +E     L  PD       IPR+RR SS A   E+ K E E    +LEAA IL+
Sbjct: 1   MGMWKTVESFRMGLDLPDLNE--IYIPRRRRGSSGAGKKEQPKTETELPLTVLEAAAILI 60

Query: 61  AMANGGFCNNEIELNNRKRKKP----PLALTKKQIRKKRRKEIKLPSTPEMPAATRDRIA 120
            MAN         +N  KRKKP    P  +TKKQ RKK+ K+ K P   EMP A RDRI 
Sbjct: 61  EMANFVSDVEPQNINTHKRKKPPTPTPTTMTKKQ-RKKKAKQSKYPWITEMPVAMRDRIV 120

Query: 121 EIGGYGIQLVI----QHTDLNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKK 180
           E+GGYGI LVI    Q TDLNKN GRLSLP +KL   F TE E+ LL + E         
Sbjct: 121 EMGGYGINLVIQKQLQDTDLNKNQGRLSLPAEKLLFDFTTEDEKILLSQRE--------- 180

Query: 181 KKKKKKGMEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWC 240
             KKKKGM V+IMD VL ER I LK WKIGSGD YCLMT WNS VEE+ L+SG+ IQ+WC
Sbjct: 181 -NKKKKGMNVLIMDDVLEERRICLKMWKIGSGDVYCLMTQWNSFVEETGLKSGEHIQIWC 240

Query: 241 FRKNCFDSETQTIRVRLCFAMLKLH 251
           FRK   D E +    RLCFAM+KL+
Sbjct: 241 FRK---DDEFEA--SRLCFAMVKLN 247

BLAST of MS016870 vs. NCBI nr
Match: XP_023540870.1 (putative B3 domain-containing protein At3g24850 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206.1 bits (523), Expect = 3.6e-49
Identity = 127/262 (48.47%), Postives = 170/262 (64.89%), Query Frame = 0

Query: 12  ELSFPDSTHGVCNIPRKRRSSSSAEESKPEDEAMLEAARILMAMANGGF----------- 71
           E+SF D   GV +IPR++R SS +  +K    +M+E A IL+A +N              
Sbjct: 9   EISFLDLDLGV-HIPRRKRGSSGSAITK-RSISMMECATILIAFSNSVTHVLPSAYLPVR 68

Query: 72  CNNEIELNNRK---------RKKPPLALTKKQIRKKRRKEIKLPSTPEMPAATRDRIAEI 131
             N   + +RK         ++KP   +T+++ RKKR K+   P+TP+MPAA RDRI E+
Sbjct: 69  TTNTKPITDRKPISERKPITKRKP---ITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEM 128

Query: 132 GGYGIQLVI----QHTDLNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKK 191
           G Y I+L I    Q TD+NKNHGRLS P KKL+V FATE+ER++L          K+++ 
Sbjct: 129 GAYQIELAIQKQLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVL----------KQQEN 188

Query: 192 KKKKGMEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFR 250
           K KKGM V+I+DP+LRE  I LK WKIGSGD YCLMT WNSLVE++  +SGD +QLW FR
Sbjct: 189 KGKKGMNVMIVDPLLRESSICLKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFR 248

BLAST of MS016870 vs. NCBI nr
Match: XP_022959448.1 (putative B3 domain-containing protein At3g24850 [Cucurbita moschata])

HSP 1 Score: 205.7 bits (522), Expect = 4.7e-49
Identity = 125/259 (48.26%), Postives = 165/259 (63.71%), Query Frame = 0

Query: 12  ELSFPDSTHGVCNIPRKRRSSSSAEESKPEDEAMLEAARILMAMA--------------- 71
           ++SF D   GV +IPR++R SS +  +K    +M+E A IL+A +               
Sbjct: 9   DISFLDLDLGV-HIPRRKRGSSGSAITK-RSISMMECATILIAFSNSVTHVLPSAYLPLR 68

Query: 72  --NGGFCNNEIELNNRKRKKPPLALTKKQIRKKRRKEIKLPSTPEMPAATRDRIAEIGGY 131
             NG    N   +  RK       +T+++ RKKR K+   P+TP+MPAA RDRI E+G Y
Sbjct: 69  TTNGKPITNTKPITKRKPITERKPITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAY 128

Query: 132 GIQLVI----QHTDLNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKKKKK 191
            IQL I    Q TD+NKNHGRLS P KKL+V FATE+ER++L          K+++ K K
Sbjct: 129 QIQLAIQKQLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVL----------KQQENKGK 188

Query: 192 KGMEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFRKNC 250
           KGM V+I+DP+LRE  I LK WKIGSGD YCLMT WNSLVE++  +SGD +QLW FRK+ 
Sbjct: 189 KGMNVMIVDPLLRESSICLKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFRKDE 248

BLAST of MS016870 vs. ExPASy Swiss-Prot
Match: Q8RV83 (B3 domain-containing protein At2g31720 OS=Arabidopsis thaliana OX=3702 GN=ARF70 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-04
Identity = 41/160 (25.62%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 73  KKPPLALTKKQIRKKRRKEIKLPSTPEMPAA-TRDRIAEIGGYGIQL----VIQHTDLNK 132
           K+P   ++ K +R   RK + +      P     D + E+ G   +L    V+ ++D+++
Sbjct: 103 KRPQKVVSNKPVR--NRKPVIVTDEDRTPTEWLIDVMREVNGMDAKLIFVKVLPNSDVDE 162

Query: 133 NHGRLSLPTKK-LRVYFATEQERKLLYEHEKKKKKKKKKKKKKKKGMEVVIMDPVLRERP 192
              RL +P K+ L + F  E+E + +  H        KK     KG +V++++    +R 
Sbjct: 163 LQTRLMMPWKQILDMDFLNEEELEKIDRHH-------KKISASDKGADVIVVNSKGLQRK 222

Query: 193 IWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCF 227
           + LK+W + S   Y L   WN +V ++ L+ G  +++W F
Sbjct: 223 LKLKRWDMTSTSNYVLGLGWNKVVTDNILQRGTRLRIWSF 253

BLAST of MS016870 vs. ExPASy TrEMBL
Match: A0A6J1CX35 (putative B3 domain-containing protein At2g27410 OS=Momordica charantia OX=3673 GN=LOC111015593 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 2.9e-60
Identity = 152/280 (54.29%), Postives = 190/280 (67.86%), Query Frame = 0

Query: 1   MGMDMI---EGLLPELSFPDSTHGVCNIPRKRR---SSSSAEES-----KPEDEA----- 60
           MGM  +   E L   + F D T+G+ +IP++RR   SSSSAE S     KP   A     
Sbjct: 1   MGMGTVVELEALKMGICFSDLTNGI-SIPQRRRTGSSSSSAETSETNKPKPSSSATAPMS 60

Query: 61  MLEAARILMAMANG-----GFCNNEIELNNRKRKKPPLALTKKQI--RKKRRKEIKLPST 120
           M+EAA +L+AMA        FC +E+E N+RKRK+ P++ ++ ++  RKK+RK ++LP T
Sbjct: 61  MVEAAAVLLAMAYSATDVEAFCKSELEKNSRKRKRDPVSKSEPEMKQRKKKRKAMELPVT 120

Query: 121 PEMPAATRDRIAEIGGYGIQLVI----QHTDLNKNHGRLSLPTKKLRVY-FATEQERKLL 180
           PEMPAA RDRI EIGGYGIQLVI    Q+TD+NKNHGRLSLP KKLR++ F TE+ERKLL
Sbjct: 121 PEMPAAMRDRIVEIGGYGIQLVIQKQLQNTDMNKNHGRLSLPAKKLRIHDFVTEEERKLL 180

Query: 181 YEHEKKKKKKKKKKKKKKKGMEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEE 240
            +H              KKGM+V I+DP L+ER I LKKWKIGSGD YCLMT WNS VEE
Sbjct: 181 NQH---------SNNNNKKGMDVPIIDPDLKERRICLKKWKIGSGDLYCLMTQWNSFVEE 240

Query: 241 SALRSGDFIQLWCFRKNCFDSETQTIRV---RLCFAMLKL 250
           +ALRSGDFIQLW FRK+ FD   +T ++   RL  AM+KL
Sbjct: 241 TALRSGDFIQLWSFRKDEFDEAEETEKISTSRLWLAMVKL 270

BLAST of MS016870 vs. ExPASy TrEMBL
Match: A0A6J1H5Z0 (putative B3 domain-containing protein At3g24850 OS=Cucurbita moschata OX=3662 GN=LOC111460418 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 2.3e-49
Identity = 125/259 (48.26%), Postives = 165/259 (63.71%), Query Frame = 0

Query: 12  ELSFPDSTHGVCNIPRKRRSSSSAEESKPEDEAMLEAARILMAMA--------------- 71
           ++SF D   GV +IPR++R SS +  +K    +M+E A IL+A +               
Sbjct: 9   DISFLDLDLGV-HIPRRKRGSSGSAITK-RSISMMECATILIAFSNSVTHVLPSAYLPLR 68

Query: 72  --NGGFCNNEIELNNRKRKKPPLALTKKQIRKKRRKEIKLPSTPEMPAATRDRIAEIGGY 131
             NG    N   +  RK       +T+++ RKKR K+   P+TP+MPAA RDRI E+G Y
Sbjct: 69  TTNGKPITNTKPITKRKPITERKPITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAY 128

Query: 132 GIQLVI----QHTDLNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKKKKK 191
            IQL I    Q TD+NKNHGRLS P KKL+V FATE+ER++L          K+++ K K
Sbjct: 129 QIQLAIQKQLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVL----------KQQENKGK 188

Query: 192 KGMEVVIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFRKNC 250
           KGM V+I+DP+LRE  I LK WKIGSGD YCLMT WNSLVE++  +SGD +QLW FRK+ 
Sbjct: 189 KGMNVMIVDPLLRESSICLKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFRKDE 248

BLAST of MS016870 vs. ExPASy TrEMBL
Match: A0A6J1JKC5 (B3 domain-containing protein At2g32645-like OS=Cucurbita maxima OX=3661 GN=LOC111487692 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.9e-48
Identity = 124/243 (51.03%), Postives = 162/243 (66.67%), Query Frame = 0

Query: 12  ELSFPDSTHGVCNIPRKRRSSSSAEESKPEDEAMLEAARILMAMANG-GFCNNEIELNNR 71
           E+SF     GV +IPR++R SS +  +K    +M+E A IL+A +N          L  R
Sbjct: 9   EISFLGLDLGV-HIPRRKRGSSGSAITK-HSISMMECATILIAFSNSVTHVLPSAYLPLR 68

Query: 72  KRKKPPLALTKKQIRKKRRKEIKLPSTPEMPAATRDRIAEIGGYGIQLVI----QHTDLN 131
                P  +T+++ RKKR K+   P+TP+MPAA RDRI E+G Y IQL I    Q TD+N
Sbjct: 69  TTNTKP--ITERKPRKKRCKQTPFPTTPDMPAAMRDRIGEMGAYQIQLAIQKQLQDTDMN 128

Query: 132 KNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKKKKKKGMEVVIMDPVLRERP 191
           KNHGRLS P KKL+V FATE+ER++L          K+++   KKGM V+I+DP+LRE  
Sbjct: 129 KNHGRLSFPAKKLKVDFATEEERRVL----------KQQENGGKKGMNVMIVDPLLRESS 188

Query: 192 IWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFRKNCFDSETQTIRVRLCFAM 250
           I LK WKIGSGD YCLMT WNSLVE++  +SGD +QLW FRK+ F++ETQT+  RLCFAM
Sbjct: 189 ICLKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQLWSFRKDEFEAETQTMVSRLCFAM 237

BLAST of MS016870 vs. ExPASy TrEMBL
Match: A0A6J1FG19 (B3 domain-containing protein At2g31420-like OS=Cucurbita moschata OX=3662 GN=LOC111445162 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 5.0e-44
Identity = 119/245 (48.57%), Postives = 148/245 (60.41%), Query Frame = 0

Query: 12  ELSFPDSTHGVCNIPRKRRSSSS---AEESKPEDEAMLEAARILMAMANGGFCNNEIELN 71
           E+S PD       IP++RR S S    E+ K + E  L+AA IL+ ++       E   N
Sbjct: 2   EVSIPDLNQ--IYIPKRRRGSCSIQRREQIKEDTEPALQAASILIYLSKAVSDIEEKISN 61

Query: 72  NRKRKKPPLALTKKQIRKKRRKEIKLPSTPEMPAATRDRIAEIGGYGIQLVI----QHTD 131
            RKRK PP   TK  +RKK  K+ + PSTPEM A  R+RI E+GG  IQLV+    QHTD
Sbjct: 62  TRKRKTPP---TKAPVRKKMLKKTEYPSTPEMSAGLRERIVEMGGSQIQLVVQKQLQHTD 121

Query: 132 LNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKKKKKKGMEVVIMDPVLRE 191
           ++KNHGRLSLP  KLR  F TE+ER+ L EH+           K K+GM VVI+D V  E
Sbjct: 122 VSKNHGRLSLPINKLRFDFTTEEERRFLNEHD----------NKNKRGMNVVIVDDVGGE 181

Query: 192 RPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFRKNCFDSETQTIRVRLCF 250
             I LKKWKIGS D YCLMT WN+LVE + LR+GD IQLW FRK+           +LC 
Sbjct: 182 SNICLKKWKIGSSDTYCLMTQWNALVEATGLRAGDHIQLWSFRKD---------EAQLCL 222

BLAST of MS016870 vs. ExPASy TrEMBL
Match: A0A5A7VC33 (Putative B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005250 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 7.2e-43
Identity = 120/253 (47.43%), Postives = 159/253 (62.85%), Query Frame = 0

Query: 9   LLPELSFPDSTHGVCNIPRKRRSSSSAEESKPEDE----AMLEAARILMAMANGGFCNNE 68
           ++PE        G  +IPR+++S S   ESK EDE    +M+EAA+IL+AMANGGFC  +
Sbjct: 9   MIPEAHSLVLIDGGVHIPRRKKSGS--VESKKEDEGASISMVEAAKILLAMANGGFCERK 68

Query: 69  IELNN--RKRKKPPLALTKKQ--IRKKRRKEIKL-PSTPEMPAATRDRIAEIGGYGIQLV 128
            + +   ++ KK P A  +K+    K+R+K ++L  S PEMPA+ RDRI   GGY IQLV
Sbjct: 69  RKKSPTVKQSKKSPTAKQRKKSPSEKQRKKSMELEESFPEMPASMRDRIVGNGGYEIQLV 128

Query: 129 IQ----HTDLNKNHGRLSLPTKKLRVYFATEQERKLLYEHEKKKKKKKKKKKKKKKGMEV 188
           IQ     +D+++NHGRL LP  K++  F  E+ER +L E       +   KKK KKG+EV
Sbjct: 129 IQKQLEESDVSRNHGRLCLPATKVKTEFVREEERNILEE-------ENNGKKKNKKGLEV 188

Query: 189 VIMDPVLRERPIWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCFRKNCFDSET 248
            +M   LRE  + LKKWKIGSG  YCLM NWN  VEE+ LRSGD+IQLW FR N      
Sbjct: 189 GVMGDDLRESSMCLKKWKIGSGKFYCLMKNWNCFVEENGLRSGDYIQLWSFRNN------ 245

BLAST of MS016870 vs. TAIR 10
Match: AT2G31720.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 48.5 bits (114), Expect = 9.0e-06
Identity = 41/160 (25.62%), Postives = 78/160 (48.75%), Query Frame = 0

Query: 73  KKPPLALTKKQIRKKRRKEIKLPSTPEMPAA-TRDRIAEIGGYGIQL----VIQHTDLNK 132
           K+P   ++ K +R   RK + +      P     D + E+ G   +L    V+ ++D+++
Sbjct: 103 KRPQKVVSNKPVR--NRKPVIVTDEDRTPTEWLIDVMREVNGMDAKLIFVKVLPNSDVDE 162

Query: 133 NHGRLSLPTKK-LRVYFATEQERKLLYEHEKKKKKKKKKKKKKKKGMEVVIMDPVLRERP 192
              RL +P K+ L + F  E+E + +  H        KK     KG +V++++    +R 
Sbjct: 163 LQTRLMMPWKQILDMDFLNEEELEKIDRHH-------KKISASDKGADVIVVNSKGLQRK 222

Query: 193 IWLKKWKIGSGDPYCLMTNWNSLVEESALRSGDFIQLWCF 227
           + LK+W + S   Y L   WN +V ++ L+ G  +++W F
Sbjct: 223 LKLKRWDMTSTSNYVLGLGWNKVVTDNILQRGTRLRIWSF 253