MS016858 (gene) Bitter gourd (TR) v1

Overview


Name	MS016858
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	zingipain-2 isoform X1
Location	scaffold9_1: 525672 .. 527800 (+)
RNA-Seq Expression	MS016858
Synteny	MS016858

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCGGAGCTGTTCGAAATCTGGTGCGCGGAACATGGGAAAACGTATTCCTCCGCCGAAGAAAAGCTGTACAGGTTCGGTGTTTTTGCCGATAACTATGATTTTGTTTCTCATCACAATAATTTGGGCGATTCTTCTTATGCCCTATCTCTCAATGCTTTTGCGGATCTTACGCACCACGAGTTCAAGGCTTCTCGCCTCGGGTTTTCCCTTTCCGCTGCTTTGCGGAACTCGCAGCCGGTTCCGCCGCAGGAACCGTCGCCTCCGCTGGATGTTCCTGATTCGTTAGATTGGAGGAAAGAAGGAGCAGTGACGGCTGTCAAGGATCAAGGAAGCTGCGGTATTTTTATATGCTATGTTTTTCCTTTTTTTTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCAGGAAAAAAAAAAGAAAAAAAAAAAGAAAGAAACCCTAGGGTCAATTTTGATGATCACAATGCATTATATGACCTGGGAATCCCTGAAAAGGGGTAAATTAGCTTTAGAATATTGCGGTTGCTCTTTCAAATCCATTCCAAGAAGCCATTTGTAGTGGGAATTGTGCGTGGAATTCTTCTGTTTCTTGAAACAGAACATCATTTTATTTAATGCATTACTTTTGAGGTGCCGAAGTTATGCTGAAGCTTTGCTCATCTAAATGGGGGCAAACTGATGGCTCTTGTTTTCAGGTGCTTGTTGGTCTTTCGCAGCAACAGGAGCTATTGAAGGAATCAATAAAATTAAAACGGGGTCTCTTATCAGTCTCTCTGAACAAGAATTAGTTGATTGTGACAGATTGCACAACTCTGGCTGCGCAGGAGGACTTATGGATTATGCATACCAATTTGTGATCACTAACCATGGGATTGACACGGAGGACGATTATCCATACCAAGGTCGTGATGGATCGTGTCGTAAGGACAAGGTAATAATTCATTTGTCATTTTGAGGGAAGTGCTTTATAAACATATGGATGAAAATAACGTATTTTATTTAGTCTCGTCATGCCTACTATCACTAGTTATTTCGGTTTTTTACTAGCAGTTTAACTTTTAACTATAACCTCAGCTTTATTTATCGGTCTAAACAAGATACGACTTATTTGATTGAAATGAATTGAATGCGCAATTCTAAAAAAAGAAACATTTCTGTGTCTTCCTCATTCATAAGAACAGGCTTGAAACTGTTAATAATACTTCTTTTACTTTTTTGAGTTGTTGGATCGGATAAGTTGTTCAAACCTTTTGATCTCTACCTGAACCGATAAGAAAAAAAGATCGCTTCTTATGGACTAGTTGATAATAATTCTTATATAATCCATGGCCATTTATAAATTGAAAATGCTATGCTAGGATCACAACAATTGCTTGCTGACTTGCATTTTGTGTCTATTGAACTTGCAGCTGAAGAGGAATGTTGTGACCATTGATGGCTACATTGATGTTGCTCCCAGTGATGAGAAAAGATTGCGGGAAGCCGTAGCAGTTCAACCTGTGAGTGTTGGTATATGTGGCAGTGAAAGAGCCTTTCAATTATACTCAAAGGTCGGTCATATCTCAACTGTAGTTTGATATTGTTCCGCTCGTTATATGTGAAGTTATTACAAAATCAAGAGCTGTATTTACTTGATAGGGAATTTTCTCCGGCCCGTGTTCAACTTCTCTGGATCATGCTGTGTTGATTGTAGGATATGGATCAGAAAACGGAGTTGATTATTGGATTGTGAAGAACTCATGGGGTAAAAGTTGGGGAATGAATGGTTATATGCACATGCAGCGAAACAGTGGCAATTCCGAAGGTGTTTGCGGAATCAACTTGCTTGCCTCATATCCAACTAAAACTAGTCCCAACCCTCCTCCCTCCCCTCCTCCAGGTCCAACAAAATGTAGTCTTCTTACTAGCTGTGCTGCTGGGGAGACCTGTTGTTGTGCAAAGAAATTTCTCGGCCTTTGTTTATCATGGAAATGTTGCGGATTGAGCTCTGCCATATGTTGCAAGGACGGTCGTCATTGTTGCCCCTTCGATTACCCAATTTGCGATATGAAGAGGAGTCTATGTCTC

mRNA sequence

CTTTCGGAGCTGTTCGAAATCTGGTGCGCGGAACATGGGAAAACGTATTCCTCCGCCGAAGAAAAGCTGTACAGGTTCGGTGTTTTTGCCGATAACTATGATTTTGTTTCTCATCACAATAATTTGGGCGATTCTTCTTATGCCCTATCTCTCAATGCTTTTGCGGATCTTACGCACCACGAGTTCAAGGCTTCTCGCCTCGGGTTTTCCCTTTCCGCTGCTTTGCGGAACTCGCAGCCGGTTCCGCCGCAGGAACCGTCGCCTCCGCTGGATGTTCCTGATTCGTTAGATTGGAGGAAAGAAGGAGCAGTGACGGCTGTCAAGGATCAAGGAAGCTGCGGTGCTTGTTGGTCTTTCGCAGCAACAGGAGCTATTGAAGGAATCAATAAAATTAAAACGGGGTCTCTTATCAGTCTCTCTGAACAAGAATTAGTTGATTGTGACAGATTGCACAACTCTGGCTGCGCAGGAGGACTTATGGATTATGCATACCAATTTGTGATCACTAACCATGGGATTGACACGGAGGACGATTATCCATACCAAGGTCGTGATGGATCGTGTCGTAAGGACAAGCTGAAGAGGAATGTTGTGACCATTGATGGCTACATTGATGTTGCTCCCAGTGATGAGAAAAGATTGCGGGAAGCCGTAGCAGTTCAACCTGTGAGTGTTGGTATATGTGGCAGTGAAAGAGCCTTTCAATTATACTCAAAGGGAATTTTCTCCGGCCCGTGTTCAACTTCTCTGGATCATGCTGTGTTGATTGTAGGATATGGATCAGAAAACGGAGTTGATTATTGGATTGTGAAGAACTCATGGGGTAAAAGTTGGGGAATGAATGGTTATATGCACATGCAGCGAAACAGTGGCAATTCCGAAGGTGTTTGCGGAATCAACTTGCTTGCCTCATATCCAACTAAAACTAGTCCCAACCCTCCTCCCTCCCCTCCTCCAGGTCCAACAAAATGTAGTCTTCTTACTAGCTGTGCTGCTGGGGAGACCTGTTGTTGTGCAAAGAAATTTCTCGGCCTTTGTTTATCATGGAAATGTTGCGGATTGAGCTCTGCCATATGTTGCAAGGACGGTCGTCATTGTTGCCCCTTCGATTACCCAATTTGCGATATGAAGAGGAGTCTATGTCTC

Coding sequence (CDS)

CTTTCGGAGCTGTTCGAAATCTGGTGCGCGGAACATGGGAAAACGTATTCCTCCGCCGAAGAAAAGCTGTACAGGTTCGGTGTTTTTGCCGATAACTATGATTTTGTTTCTCATCACAATAATTTGGGCGATTCTTCTTATGCCCTATCTCTCAATGCTTTTGCGGATCTTACGCACCACGAGTTCAAGGCTTCTCGCCTCGGGTTTTCCCTTTCCGCTGCTTTGCGGAACTCGCAGCCGGTTCCGCCGCAGGAACCGTCGCCTCCGCTGGATGTTCCTGATTCGTTAGATTGGAGGAAAGAAGGAGCAGTGACGGCTGTCAAGGATCAAGGAAGCTGCGGTGCTTGTTGGTCTTTCGCAGCAACAGGAGCTATTGAAGGAATCAATAAAATTAAAACGGGGTCTCTTATCAGTCTCTCTGAACAAGAATTAGTTGATTGTGACAGATTGCACAACTCTGGCTGCGCAGGAGGACTTATGGATTATGCATACCAATTTGTGATCACTAACCATGGGATTGACACGGAGGACGATTATCCATACCAAGGTCGTGATGGATCGTGTCGTAAGGACAAGCTGAAGAGGAATGTTGTGACCATTGATGGCTACATTGATGTTGCTCCCAGTGATGAGAAAAGATTGCGGGAAGCCGTAGCAGTTCAACCTGTGAGTGTTGGTATATGTGGCAGTGAAAGAGCCTTTCAATTATACTCAAAGGGAATTTTCTCCGGCCCGTGTTCAACTTCTCTGGATCATGCTGTGTTGATTGTAGGATATGGATCAGAAAACGGAGTTGATTATTGGATTGTGAAGAACTCATGGGGTAAAAGTTGGGGAATGAATGGTTATATGCACATGCAGCGAAACAGTGGCAATTCCGAAGGTGTTTGCGGAATCAACTTGCTTGCCTCATATCCAACTAAAACTAGTCCCAACCCTCCTCCCTCCCCTCCTCCAGGTCCAACAAAATGTAGTCTTCTTACTAGCTGTGCTGCTGGGGAGACCTGTTGTTGTGCAAAGAAATTTCTCGGCCTTTGTTTATCATGGAAATGTTGCGGATTGAGCTCTGCCATATGTTGCAAGGACGGTCGTCATTGTTGCCCCTTCGATTACCCAATTTGCGATATGAAGAGGAGTCTATGTCTC

Protein sequence

LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHHEFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFAATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYPYQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGINLLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICCKDGRHCCPFDYPICDMKRSLCL

Homology

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BLAST of MS016858 vs. NCBI nr
Match: XP_022144360.1 (zingipain-2 isoform X1 [Momordica charantia])

HSP 1 Score: 802.4 bits (2071), Expect = 1.7e-228
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH
Sbjct: 28  LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 87

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA
Sbjct: 88  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 147

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP
Sbjct: 148 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 207

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG
Sbjct: 208 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 267

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN
Sbjct: 268 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 327

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
           LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC
Sbjct: 328 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 387

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICDMKRSLCL
Sbjct: 388 KDGRHCCPFDYPICDMKRSLCL 409

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BLAST of MS016858 vs. NCBI nr
Match: XP_008444761.1 (PREDICTED: zingipain-2 [Cucumis melo])

HSP 1 Score: 716.5 bits (1848), Expect = 1.3e-202
Identity = 335/382 (87.70%), Postives = 359/382 (93.98%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR  VFADNY+FV+HHNNLG+SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFVTHHNNLGNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S ALRN +PV PQEPS P DVPDSLDWRK+GAVTAVKDQGSCGACWSF+
Sbjct: 85  EFKVSRLGF--SPALRNFRPVLPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSCGACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGIN+I TGSLIS+SEQEL+DCDR +NSGC GGLMDYAYQFVI+NHGIDTEDDYP
Sbjct: 145 ATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVISNHGIDTEDDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQGRDGSCRKDKL+RNVVTIDGY D+ P+DE +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYMHMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
            LASYPTKTSPNPPPSPPPGPTKCS+LTSCAAGETCCCAKKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 KLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCCAKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 404

[Close]

BLAST of MS016858 vs. NCBI nr
Match: KAA0065198.1 (zingipain-2 [Cucumis melo var. makuwa])

HSP 1 Score: 709.9 bits (1831), Expect = 1.2e-200
Identity = 334/382 (87.43%), Postives = 358/382 (93.72%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR  VFADNY+FV+HHNNLG+SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFVTHHNNLGNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S ALRN +PV PQEPS P DVPDSLDWRK+GAVTAVKDQGSC ACWSF+
Sbjct: 85  EFKVSRLGF--SPALRNFRPVLPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSC-ACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGIN+I TGSLIS+SEQEL+DCDR +NSGC GGLMDYAYQFVI+NHGIDTEDDYP
Sbjct: 145 ATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVISNHGIDTEDDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQGRDGSCRKDKL+RNVVTIDGY D+ P+DE +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYMHMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
            LASYPTKTSPNPPPSPPPGPTKCS+LTSCAAGETCCCAKKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 KLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCCAKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 403

[Close]

BLAST of MS016858 vs. NCBI nr
Match: XP_004152671.1 (zingipain-2 [Cucumis sativus] >KGN62639.1 hypothetical protein Csa_022234 [Cucumis sativus])

HSP 1 Score: 707.6 bits (1825), Expect = 5.8e-200
Identity = 331/382 (86.65%), Postives = 357/382 (93.46%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR GVFADNY+FV+HHNNL +SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNLDNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S ALRN +PV PQEPS P DVPDSLDWRK+GAVTAVKDQGSCGACWSF+
Sbjct: 85  EFKVSRLGF--SPALRNFRPVLPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSCGACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGA+EGIN+I TGSLISLSEQEL+DCDR +NSGC GGLMDYAYQFVI+NHGIDTE+DYP
Sbjct: 145 ATGAMEGINQIMTGSLISLSEQELIDCDRSYNSGCGGGLMDYAYQFVISNHGIDTENDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQ RDGSCRKDKL+RNVVTIDGY D+  +DE +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQARDGSCRKDKLQRNVVTIDGYADIPSNDEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYMHMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
            LASYPTKT+PNPPPSPPPGPTKCS+LTSCAAGETCCCAKKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 KLASYPTKTNPNPPPSPPPGPTKCSILTSCAAGETCCCAKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 404

[Close]

BLAST of MS016858 vs. NCBI nr
Match: XP_038885598.1 (zingipain-2 [Benincasa hispida])

HSP 1 Score: 701.8 bits (1810), Expect = 3.2e-198
Identity = 328/382 (85.86%), Postives = 355/382 (92.93%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR  VFADNY+FV+HHN+LG+SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFVTHHNSLGNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S+A RN + VPPQEPS P DVPDSLDWRK+GAVTAVKDQGSCGACWSF+
Sbjct: 85  EFKVSRLGF--SSASRNLRRVPPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSCGACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGIN+I TGSLISLSEQEL+DCDR +NSGC GGLMDYAYQFVI NHGIDTE+DYP
Sbjct: 145 ATGAIEGINQIMTGSLISLSEQELIDCDRSYNSGCGGGLMDYAYQFVIQNHGIDTENDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQG DGSCRKDKLKRN VTIDGY D+ P++E +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQGHDGSCRKDKLKRNAVTIDGYTDIPPNNEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYM MQRNSG+SEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMLMQRNSGSSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
           +LASYPTKTSPNPPPSPPPGPTKCS+LTSCAAGETCCC KKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 MLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCCTKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 404

[Close]

BLAST of MS016858 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.6e-115
Identity = 201/382 (52.62%), Postives = 257/382 (67.28%), Query Frame = 0


Query: 4   LFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHHEFK 63
           ++E W  E+ K Y+   EK  RF +F DN  FV  H+++ + +Y + L  FADLT+ EF+
Sbjct: 42  MYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFR 101

Query: 64  ASRLGFSLSAALRNSQPVPPQEPSPPL--DVPDSLDWRKEGAVTAVKDQGSCGACWSFAA 123
           A  L   +    R   PV  ++    +   +PD++DWR +GAV  VKDQGSCG+CW+F+A
Sbjct: 102 AIYLRSKME---RTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSA 161

Query: 124 TGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYPY 183
            GA+EGIN+IKTG LISLSEQELVDCD  +N GC GGLMDYA++F+I N GIDTE+DYPY
Sbjct: 162 IGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPY 221

Query: 184 QGRD-GSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 243
              D   C  DK    VVTIDGY DV  +DEK L++A+A QP+SV I    RAFQLY+ G
Sbjct: 222 IATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSG 281

Query: 244 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 303
           +F+G C TSLDH V+ VGYGSE G DYWIV+NSWG +WG +GY  ++RN   S G CG+ 
Sbjct: 282 VFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVA 341

Query: 304 LLASYPTKTSPNPPPSPP-PGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAIC 363
           ++ASYPTK+S + PP PP P P  C    +C A  TCCC  ++ G C SW CC   SA C
Sbjct: 342 MMASYPTKSSGSNPPKPPAPSPVVCDKSNTCPAKSTCCCLYEYNGKCYSWGCCPYESATC 401

Query: 364 CKDGRHCCPFDYPICDMKRSLC 382
           C DG  CCP  YP+CD+K + C
Sbjct: 402 CDDGSSCCPQSYPVCDLKANTC 420

[Close]

BLAST of MS016858 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 402.9 bits (1034), Expect = 4.0e-111
Identity = 200/389 (51.41%), Postives = 255/389 (65.55%), Query Frame = 0


Query: 4   LFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDS---SYALSLNAFADLTHH 63
           L+  W AEHGK+Y++  E+  R+  F DN  ++  HN   D+   S+ L LN FADLT+ 
Sbjct: 39  LYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNE 98

Query: 64  EFKASRLGFSLSAALRNSQPVPPQEPSPPLD-VPDSLDWRKEGAVTAVKDQGSCGACWSF 123
           E++ + LG  L    R  + V  +  +   + +P+S+DWR +GAV  +KDQG CG+CW+F
Sbjct: 99  EYRDTYLG--LRNKPRRERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQGGCGSCWAF 158

Query: 124 AATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDY 183
           +A  A+EGIN+I TG LISLSEQELVDCD  +N GC GGLMDYA+ F+I N GIDTEDDY
Sbjct: 159 SAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINNGGIDTEDDY 218

Query: 184 PYQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSK 243
           PY+G+D  C  ++    VVTID Y DV P+ E  L++AVA QPVSV I    RAFQLYS 
Sbjct: 219 PYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAGGRAFQLYSS 278

Query: 244 GIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGI 303
           GIF+G C T+LDH V  VGYG+ENG DYWIV+NSWGKSWG +GY+ M+RN   S G CGI
Sbjct: 279 GIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNIKASSGKCGI 338

Query: 304 NLLASYPTKTSPNP------PPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCG 363
            +  SYP K   NP      PPSP P PT C    +C    TCCC  ++   C +W CC 
Sbjct: 339 AVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCCCIYEYGKYCYAWGCCP 398

Query: 364 LSSAICCKDGRHCCPFDYPICDMKRSLCL 383
           L  A CC D   CCP +YPIC++++  CL
Sbjct: 399 LEGATCCDDHYSCCPHEYPICNVQQGTCL 425

[Close]

BLAST of MS016858 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-109
Identity = 195/387 (50.39%), Postives = 248/387 (64.08%), Query Frame = 0


Query: 4   LFEIWCAEHGKTYS--SAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHHE 63
           ++E W  +HGK  S  S  EK  RF +F DN  FV  HN   + SY L L  FADLT+ E
Sbjct: 49  IYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNE-KNLSYRLGLTRFADLTNDE 108

Query: 64  FKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFAA 123
           +++  LG  +         +   E     ++P+S+DWRK+GAV  VKDQG CG+CW+F+ 
Sbjct: 109 YRSKYLGAKMEKKGERRTSL-RYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFST 168

Query: 124 TGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYPY 183
            GA+EGIN+I TG LI+LSEQELVDCD  +N GC GGLMDYA++F+I N GIDT+ DYPY
Sbjct: 169 IGAVEGINQIVTGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPY 228

Query: 184 QGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKGI 243
           +G DG+C + +    VVTID Y DV    E+ L++AVA QP+S+ I    RAFQLY  GI
Sbjct: 229 KGVDGTCDQIRKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGI 288

Query: 244 FSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGINL 303
           F G C T LDH V+ VGYG+ENG DYWIV+NSWGKSWG +GY+ M RN  +S G CGI +
Sbjct: 289 FDGSCGTQLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAI 348

Query: 304 LASYPTKTSPNP------PPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLS 363
             SYP K   NP      PPSP   PT+C    +C    TCCC  ++   C +W CC L 
Sbjct: 349 EPSYPIKNGENPPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLE 408

Query: 364 SAICCKDGRHCCPFDYPICDMKRSLCL 383
           +A CC D   CCP +YP+CD+ +  CL
Sbjct: 409 AATCCDDNYSCCPHEYPVCDLDQGTCL 433

[Close]

BLAST of MS016858 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.9e-109
Identity = 200/393 (50.89%), Postives = 244/393 (62.09%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSS----AEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFAD 60
           +  ++E W  EHGK   +      EK  RF +F DN  F+  HN   + SY L L  FAD
Sbjct: 46  VERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNT-KNLSYKLGLTRFAD 105

Query: 61  LTHHEFKASRLGFS-LSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGA 120
           LT+ E+++  LG       L+ S     +       +PDS+DWRKEGAV  VKDQGSCG+
Sbjct: 106 LTNEEYRSMYLGAKPTKRVLKTSDRYQARVGDA---LPDSVDWRKEGAVADVKDQGSCGS 165

Query: 121 CWSFAATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDT 180
           CW+F+  GA+EGINKI TG LISLSEQELVDCD  +N GC GGLMDYA++F+I N GIDT
Sbjct: 166 CWAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDT 225

Query: 181 EDDYPYQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQ 240
           E DYPY+  DG C +++    VVTID Y DV  + E  L++A+A QP+SV I    RAFQ
Sbjct: 226 EADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQ 285

Query: 241 LYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEG 300
           LYS G+F G C T LDH V+ VGYG+ENG DYWIV+NSWG  WG +GY+ M RN     G
Sbjct: 286 LYSSGVFDGLCGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTG 345

Query: 301 VCGINLLASYPTKTSPNP------PPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSW 360
            CGI + ASYP K   NP      PPSP   PT C    SC    TCCC  K+   C  W
Sbjct: 346 KCGIAMEASYPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGW 405

Query: 361 KCCGLSSAICCKDGRHCCPFDYPICDMKRSLCL 383
            CC L +A CC D   CCP +YP+CD+ R  CL
Sbjct: 406 GCCPLEAATCCDDNSSCCPHEYPVCDVNRGTCL 434

[Close]

BLAST of MS016858 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 374.4 bits (960), Expect = 1.5e-102
Identity = 195/394 (49.49%), Postives = 249/394 (63.20%), Query Frame = 0


Query: 5   FEIWCAEHGKTYSSA--EEKLYRFGVFADNYDFVSHHNNLGD--SSYALSLNAFADLTHH 64
           +++W AE+G    +A   E   RF VF DN  FV  HN   D    + L +N FADLT+ 
Sbjct: 52  YDLWLAENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNE 111

Query: 65  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 124
           EF+A+ LG     A R+             ++P+S+DWR++GAV  VK+QG CG+CW+F+
Sbjct: 112 EFRATFLG--AKVAERSRAAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFS 171

Query: 125 ATGAIEGINKIKTGSLISLSEQELVDCD-RLHNSGCAGGLMDYAYQFVITNHGIDTEDDY 184
           A   +E IN++ TG +I+LSEQELV+C     NSGC GGLMD A+ F+I N GIDTEDDY
Sbjct: 172 AVSTVESINQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDY 231

Query: 185 PYQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSK 244
           PY+  DG C  ++    VV+IDG+ DV  +DEK L++AVA QPVSV I    R FQLY  
Sbjct: 232 PYKAVDGKCDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHS 291

Query: 245 GIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGI 304
           G+FSG C TSLDH V+ VGYG++NG DYWIV+NSWG  WG +GY+ M+RN   + G CGI
Sbjct: 292 GVFSGRCGTSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNINVTTGKCGI 351

Query: 305 NLLASYPTKTSPNPP---PSPPPGPTK---------CSLLTSCAAGETCCCAKKFLGLCL 364
            ++ASYPTK+  NPP   P+PP  PT          C    SC AG TCCCA  F  LCL
Sbjct: 352 AMMASYPTKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCL 411

Query: 365 SWKCCGLSSAICCKDGRHCCPFDYPICDMKRSLC 382
            W CC +  A CCKD   CCP DYP+C+ +   C
Sbjct: 412 VWGCCPVEGATCCKDHASCCPPDYPVCNTRAGTC 443

[Close]

BLAST of MS016858 vs. ExPASy TrEMBL
Match: A0A6J1CRE7 (zingipain-2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014064 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 8.4e-229
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH
Sbjct: 28  LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 87

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA
Sbjct: 88  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 147

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP
Sbjct: 148 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 207

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG
Sbjct: 208 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 267

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN
Sbjct: 268 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 327

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
           LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC
Sbjct: 328 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 387

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICDMKRSLCL
Sbjct: 388 KDGRHCCPFDYPICDMKRSLCL 409

[Close]

BLAST of MS016858 vs. ExPASy TrEMBL
Match: A0A1S3BBY8 (zingipain-2 OS=Cucumis melo OX=3656 GN=LOC103488009 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.1e-203
Identity = 335/382 (87.70%), Postives = 359/382 (93.98%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR  VFADNY+FV+HHNNLG+SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFVTHHNNLGNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S ALRN +PV PQEPS P DVPDSLDWRK+GAVTAVKDQGSCGACWSF+
Sbjct: 85  EFKVSRLGF--SPALRNFRPVLPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSCGACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGIN+I TGSLIS+SEQEL+DCDR +NSGC GGLMDYAYQFVI+NHGIDTEDDYP
Sbjct: 145 ATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVISNHGIDTEDDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQGRDGSCRKDKL+RNVVTIDGY D+ P+DE +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYMHMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
            LASYPTKTSPNPPPSPPPGPTKCS+LTSCAAGETCCCAKKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 KLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCCAKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 404

[Close]

BLAST of MS016858 vs. ExPASy TrEMBL
Match: A0A5A7VC45 (Zingipain-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005350 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.7e-201
Identity = 334/382 (87.43%), Postives = 358/382 (93.72%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR  VFADNY+FV+HHNNLG+SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLSVFADNYEFVTHHNNLGNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S ALRN +PV PQEPS P DVPDSLDWRK+GAVTAVKDQGSC ACWSF+
Sbjct: 85  EFKVSRLGF--SPALRNFRPVLPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSC-ACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGIN+I TGSLIS+SEQEL+DCDR +NSGC GGLMDYAYQFVI+NHGIDTEDDYP
Sbjct: 145 ATGAIEGINQIMTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVISNHGIDTEDDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQGRDGSCRKDKL+RNVVTIDGY D+ P+DE +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQGRDGSCRKDKLQRNVVTIDGYTDIPPNDEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYMHMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
            LASYPTKTSPNPPPSPPPGPTKCS+LTSCAAGETCCCAKKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 KLASYPTKTSPNPPPSPPPGPTKCSILTSCAAGETCCCAKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 403

[Close]

BLAST of MS016858 vs. ExPASy TrEMBL
Match: A0A0A0LNP7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362510 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 2.8e-200
Identity = 331/382 (86.65%), Postives = 357/382 (93.46%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR GVFADNY+FV+HHNNL +SSY LSLN++ADLTHH
Sbjct: 25  VSELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNLDNSSYTLSLNSYADLTHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK SRLGF  S ALRN +PV PQEPS P DVPDSLDWRK+GAVTAVKDQGSCGACWSF+
Sbjct: 85  EFKVSRLGF--SPALRNFRPVLPQEPSLPRDVPDSLDWRKKGAVTAVKDQGSCGACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGA+EGIN+I TGSLISLSEQEL+DCDR +NSGC GGLMDYAYQFVI+NHGIDTE+DYP
Sbjct: 145 ATGAMEGINQIMTGSLISLSEQELIDCDRSYNSGCGGGLMDYAYQFVISNHGIDTENDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQ RDGSCRKDKL+RNVVTIDGY D+  +DE +L +AVA QPVSVGICGSERAFQLYSKG
Sbjct: 205 YQARDGSCRKDKLQRNVVTIDGYADIPSNDEGKLLQAVAAQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGM+GYMHMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMDGYMHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
            LASYPTKT+PNPPPSPPPGPTKCS+LTSCAAGETCCCAKKFLGLCLSWKCCGLSSA+CC
Sbjct: 325 KLASYPTKTNPNPPPSPPPGPTKCSILTSCAAGETCCCAKKFLGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD  R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTDRNLCL 404

[Close]

BLAST of MS016858 vs. ExPASy TrEMBL
Match: A0A6J1GH92 (zingipain-2 OS=Cucurbita moschata OX=3662 GN=LOC111454188 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.6e-198
Identity = 325/382 (85.08%), Postives = 358/382 (93.72%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELFEIWC EHGK+YSSAEEKLYR GVFADNY+FV+HHNN G+SSY LSLNA+AD+THH
Sbjct: 25  ISELFEIWCTEHGKSYSSAEEKLYRLGVFADNYEFVTHHNNQGNSSYTLSLNAYADITHH 84

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFKA+RLG  LS+ALR+S+PV PQEP    DVP+SLDWRK+GAVTAVKDQGSCGACWSF+
Sbjct: 85  EFKAARLG--LSSALRSSRPVSPQEPYLHRDVPESLDWRKKGAVTAVKDQGSCGACWSFS 144

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGAIEGIN+I+TGSLIS+SEQEL+DCDR +NSGC GGLMDYAYQFVI NHGIDTEDDYP
Sbjct: 145 ATGAIEGINQIRTGSLISVSEQELIDCDRSYNSGCGGGLMDYAYQFVIKNHGIDTEDDYP 204

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           +QGRDGSC KDKL R VVTIDGY DV P++E++L +AVA+QPVSVGICGSERAFQLYSKG
Sbjct: 205 FQGRDGSCHKDKLNRKVVTIDGYSDVPPNNEEKLLQAVAIQPVSVGICGSERAFQLYSKG 264

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGK WGM+GY+HMQRNSGNSEGVCGIN
Sbjct: 265 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKRWGMDGYIHMQRNSGNSEGVCGIN 324

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
           +LASYPTKTSPNPPPSPPPGPTKCS LTSCAAGETCCCAK+F GLCLSWKCCGLSSA+CC
Sbjct: 325 MLASYPTKTSPNPPPSPPPGPTKCSFLTSCAAGETCCCAKEFFGLCLSWKCCGLSSAVCC 384

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCPFDYPICD +R+LCL
Sbjct: 385 KDGRHCCPFDYPICDTQRNLCL 404

[Close]

BLAST of MS016858 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 596.7 bits (1537), Expect = 1.4e-170
Identity = 277/382 (72.51%), Postives = 320/382 (83.77%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           +SELF+ WC +HGKTY S EE+  R  +F DN+DFV+ HN + +++Y+LSLNAFADLTHH
Sbjct: 28  ISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLNAFADLTHH 87

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFKASRLG S+SA          Q     + VPDS+DWRK+GAVT VKDQGSCGACWSF+
Sbjct: 88  EFKASRLGLSVSAP-SVIMASKGQSLGGSVKVPDSVDWRKKGAVTNVKDQGSCGACWSFS 147

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
           ATGA+EGIN+I TG LISLSEQEL+DCD+ +N+GC GGLMDYA++FVI NHGIDTE DYP
Sbjct: 148 ATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVIKNHGIDTEKDYP 207

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           YQ RDG+C+KDKLK+ VVTID Y  V  +DEK L EAVA QPVSVGICGSERAFQLYS G
Sbjct: 208 YQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSERAFQLYSSG 267

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           IFSGPCSTSLDHAVLIVGYGS+NGVDYWIVKNSWGKSWGM+G+MHMQRN+ NS+GVCGIN
Sbjct: 268 IFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTENSDGVCGIN 327

Query: 301 LLASYPTKTSPNPPPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAICC 360
           +LASYP KT PNPPP  PPGPTKC+L T C++GETCCCA++  GLC SWKCC + SA+CC
Sbjct: 328 MLASYPIKTHPNPPPPSPPGPTKCNLFTYCSSGETCCCARELFGLCFSWKCCEIESAVCC 387

Query: 361 KDGRHCCPFDYPICDMKRSLCL 383
           KDGRHCCP DYP+CD  RSLCL
Sbjct: 388 KDGRHCCPHDYPVCDTTRSLCL 408

[Close]

BLAST of MS016858 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 3.3e-116
Identity = 201/382 (52.62%), Postives = 257/382 (67.28%), Query Frame = 0


Query: 4   LFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHHEFK 63
           ++E W  E+ K Y+   EK  RF +F DN  FV  H+++ + +Y + L  FADLT+ EF+
Sbjct: 42  MYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFR 101

Query: 64  ASRLGFSLSAALRNSQPVPPQEPSPPL--DVPDSLDWRKEGAVTAVKDQGSCGACWSFAA 123
           A  L   +    R   PV  ++    +   +PD++DWR +GAV  VKDQGSCG+CW+F+A
Sbjct: 102 AIYLRSKME---RTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSA 161

Query: 124 TGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYPY 183
            GA+EGIN+IKTG LISLSEQELVDCD  +N GC GGLMDYA++F+I N GIDTE+DYPY
Sbjct: 162 IGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPY 221

Query: 184 QGRD-GSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 243
              D   C  DK    VVTIDGY DV  +DEK L++A+A QP+SV I    RAFQLY+ G
Sbjct: 222 IATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSG 281

Query: 244 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 303
           +F+G C TSLDH V+ VGYGSE G DYWIV+NSWG +WG +GY  ++RN   S G CG+ 
Sbjct: 282 VFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVA 341

Query: 304 LLASYPTKTSPNPPPSPP-PGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLSSAIC 363
           ++ASYPTK+S + PP PP P P  C    +C A  TCCC  ++ G C SW CC   SA C
Sbjct: 342 MMASYPTKSSGSNPPKPPAPSPVVCDKSNTCPAKSTCCCLYEYNGKCYSWGCCPYESATC 401

Query: 364 CKDGRHCCPFDYPICDMKRSLC 382
           C DG  CCP  YP+CD+K + C
Sbjct: 402 CDDGSSCCPQSYPVCDLKANTC 420

[Close]

BLAST of MS016858 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.1e-110
Identity = 195/387 (50.39%), Postives = 248/387 (64.08%), Query Frame = 0


Query: 4   LFEIWCAEHGKTYS--SAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHHE 63
           ++E W  +HGK  S  S  EK  RF +F DN  FV  HN   + SY L L  FADLT+ E
Sbjct: 49  IYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNE-KNLSYRLGLTRFADLTNDE 108

Query: 64  FKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFAA 123
           +++  LG  +         +   E     ++P+S+DWRK+GAV  VKDQG CG+CW+F+ 
Sbjct: 109 YRSKYLGAKMEKKGERRTSL-RYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFST 168

Query: 124 TGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYPY 183
            GA+EGIN+I TG LI+LSEQELVDCD  +N GC GGLMDYA++F+I N GIDT+ DYPY
Sbjct: 169 IGAVEGINQIVTGDLITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPY 228

Query: 184 QGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKGI 243
           +G DG+C + +    VVTID Y DV    E+ L++AVA QP+S+ I    RAFQLY  GI
Sbjct: 229 KGVDGTCDQIRKNAKVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGI 288

Query: 244 FSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGINL 303
           F G C T LDH V+ VGYG+ENG DYWIV+NSWGKSWG +GY+ M RN  +S G CGI +
Sbjct: 289 FDGSCGTQLDHGVVAVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAI 348

Query: 304 LASYPTKTSPNP------PPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSWKCCGLS 363
             SYP K   NP      PPSP   PT+C    +C    TCCC  ++   C +W CC L 
Sbjct: 349 EPSYPIKNGENPPNPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLE 408

Query: 364 SAICCKDGRHCCPFDYPICDMKRSLCL 383
           +A CC D   CCP +YP+CD+ +  CL
Sbjct: 409 AATCCDDNYSCCPHEYPVCDLDQGTCL 433

[Close]

BLAST of MS016858 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.1e-110
Identity = 200/393 (50.89%), Postives = 244/393 (62.09%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSS----AEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFAD 60
           +  ++E W  EHGK   +      EK  RF +F DN  F+  HN   + SY L L  FAD
Sbjct: 46  VERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNT-KNLSYKLGLTRFAD 105

Query: 61  LTHHEFKASRLGFS-LSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGA 120
           LT+ E+++  LG       L+ S     +       +PDS+DWRKEGAV  VKDQGSCG+
Sbjct: 106 LTNEEYRSMYLGAKPTKRVLKTSDRYQARVGDA---LPDSVDWRKEGAVADVKDQGSCGS 165

Query: 121 CWSFAATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDT 180
           CW+F+  GA+EGINKI TG LISLSEQELVDCD  +N GC GGLMDYA++F+I N GIDT
Sbjct: 166 CWAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDT 225

Query: 181 EDDYPYQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQ 240
           E DYPY+  DG C +++    VVTID Y DV  + E  L++A+A QP+SV I    RAFQ
Sbjct: 226 EADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQ 285

Query: 241 LYSKGIFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEG 300
           LYS G+F G C T LDH V+ VGYG+ENG DYWIV+NSWG  WG +GY+ M RN     G
Sbjct: 286 LYSSGVFDGLCGTELDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTG 345

Query: 301 VCGINLLASYPTKTSPNP------PPSPPPGPTKCSLLTSCAAGETCCCAKKFLGLCLSW 360
            CGI + ASYP K   NP      PPSP   PT C    SC    TCCC  K+   C  W
Sbjct: 346 KCGIAMEASYPIKKGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGW 405

Query: 361 KCCGLSSAICCKDGRHCCPFDYPICDMKRSLCL 383
            CC L +A CC D   CCP +YP+CD+ R  CL
Sbjct: 406 GCCPLEAATCCDDNSSCCPHEYPVCDVNRGTCL 434

[Close]

BLAST of MS016858 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 338.6 bits (867), Expect = 6.6e-93
Identity = 168/309 (54.37%), Postives = 206/309 (66.67%), Query Frame = 0


Query: 1   LSELFEIWCAEHGKTYSSAEEKLYRFGVFADNYDFVSHHNNLGDSSYALSLNAFADLTHH 60
           L ELFE W +EH K Y S EEK++RF VF +N   +   NN   +SY L LN FADLTH 
Sbjct: 47  LLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNN-EINSYWLGLNEFADLTHE 106

Query: 61  EFKASRLGFSLSAALRNSQPVPPQEPSPPLDVPDSLDWRKEGAVTAVKDQGSCGACWSFA 120
           EFK   LG +     R  QP          D+P S+DWRK+GAV  VKDQG CG+CW+F+
Sbjct: 107 EFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFS 166

Query: 121 ATGAIEGINKIKTGSLISLSEQELVDCDRLHNSGCAGGLMDYAYQFVITNHGIDTEDDYP 180
              A+EGIN+I TG+L SLSEQEL+DCD   NSGC GGLMDYA+Q++I+  G+  EDDYP
Sbjct: 167 TVAAVEGINQITTGNLSSLSEQELIDCDTTFNSGCNGGLMDYAFQYIISTGGLHKEDDYP 226

Query: 181 YQGRDGSCRKDKLKRNVVTIDGYIDVAPSDEKRLREAVAVQPVSVGICGSERAFQLYSKG 240
           Y   +G C++ K     VTI GY DV  +D++ L +A+A QPVSV I  S R FQ Y  G
Sbjct: 227 YLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGG 286

Query: 241 IFSGPCSTSLDHAVLIVGYGSENGVDYWIVKNSWGKSWGMNGYMHMQRNSGNSEGVCGIN 300
           +F+G C T LDH V  VGYGS  G DY IVKNSWG  WG  G++ M+RN+G  EG+CGIN
Sbjct: 287 VFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGIN 346

Query: 301 LLASYPTKT 310
            +ASYPTKT
Sbjct: 347 KMASYPTKT 354

The following BLAST results are available for this feature:

BLAST of MS016858 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144360.1	1.7e-228	100.00	zingipain-2 isoform X1 [Momordica charantia]	[more]
XP_008444761.1	1.3e-202	87.70	PREDICTED: zingipain-2 [Cucumis melo]	[more]
KAA0065198.1	1.2e-200	87.43	zingipain-2 [Cucumis melo var. makuwa]	[more]
XP_004152671.1	5.8e-200	86.65	zingipain-2 [Cucumis sativus] >KGN62639.1 hypothetical protein Csa_022234 [Cucum...	[more]
XP_038885598.1	3.2e-198	85.86	zingipain-2 [Benincasa hispida]	[more]

BLAST of MS016858 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT78	4.6e-115	52.62	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25776	4.0e-111	51.41	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
P43297	2.9e-109	50.39	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	2.9e-109	50.89	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25777	1.5e-102	49.49	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]

BLAST of MS016858 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CRE7	8.4e-229	100.00	zingipain-2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014064 PE=3 SV=1	[more]
A0A1S3BBY8	6.1e-203	87.70	zingipain-2 OS=Cucumis melo OX=3656 GN=LOC103488009 PE=3 SV=1	[more]
A0A5A7VC45	5.7e-201	87.43	Zingipain-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005350 PE...	[more]
A0A0A0LNP7	2.8e-200	86.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G362510 PE=3 SV=1	[more]
A0A6J1GH92	2.6e-198	85.08	zingipain-2 OS=Cucurbita moschata OX=3662 GN=LOC111454188 PE=3 SV=1	[more]

BLAST of MS016858 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09850.1	1.4e-170	72.51	xylem bark cysteine peptidase 3	[more]
AT3G19390.1	3.3e-116	52.62	Granulin repeat cysteine protease family protein	[more]
AT1G47128.1	2.1e-110	50.39	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	2.1e-110	50.89	Granulin repeat cysteine protease family protein	[more]
AT4G35350.1	6.6e-93	54.37	xylem cysteine peptidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 252..262 score: 62.56 coord: 110..125 score: 55.66 coord: 267..273 score: 79.08
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 92..307 e-value: 9.6E-120 score: 413.8
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 92..306 e-value: 5.0E-81 score: 271.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 5..62 e-value: 2.5E-23 score: 93.5
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 5..62 e-value: 8.0E-16 score: 58.2
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 324..381 e-value: 1.0E-21 score: 88.2
IPR000118	Granulin	PFAM	PF00396	Granulin	coord: 335..381 e-value: 6.8E-10 score: 39.2
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 1..309 e-value: 7.9E-115 score: 385.7
None	No IPR available	PANTHER	PTHR12411:SF414	OS05G0508300 PROTEIN	coord: 2..334
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 2..334
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 321..354
IPR037277	Granulin superfamily	GENE3D	2.10.25.160	Granulin	coord: 321..382 e-value: 2.1E-9 score: 39.6
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 267..286
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 250..260
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 110..121
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 93..306 e-value: 4.60984E-107 score: 311.48
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 3..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016858.1	MS016858.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity