MS016836 (gene) Bitter gourd (TR) v1

BLAST of MS016836 vs. NCBI nr
Match: XP_022144159.1 (cell wall protein RBR3 [Momordica charantia])

HSP 1 Score: 869.4 bits (2245), Expect = 1.6e-248
Identity = 488/489 (99.80%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 42  MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK 101
           MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK
Sbjct: 1   MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK 60

Query: 102 DASKPSSPAVAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRP 161
           DASKPSSPAVAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRP
Sbjct: 61  DASKPSSPAVAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRP 120

Query: 162 QASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPK 221
           QASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPK
Sbjct: 121 QASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPK 180

Query: 222 GRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDP 281
           GRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDP
Sbjct: 181 GRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDP 240

Query: 282 SLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAF 341
           SLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAF
Sbjct: 241 SLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAF 300

Query: 342 PKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTE 401
           PKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTE
Sbjct: 301 PKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTE 360

Query: 402 IEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKS 461
           IEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKS
Sbjct: 361 IEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKS 420

Query: 462 EEISESLNTQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSN 521
           EEISESL+TQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSN
Sbjct: 421 EEISESLSTQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSN 480

Query: 522 CKGKDGEIM 531
           CKGKDGEIM
Sbjct: 481 CKGKDGEIM 489

BLAST of MS016836 vs. NCBI nr
Match: XP_023002262.1 (cell wall protein RBR3-like [Cucurbita maxima])

HSP 1 Score: 441.8 bits (1135), Expect = 8.3e-120
Identity = 324/605 (53.55%), Postives = 390/605 (64.46%), Query Frame = 0

Query: 1   ENKEEAQTSASAADTVSTIRHQPVQSPKIKSEDSSSSSC------ETMPPSQSKKESQVH 60
           E  +EA+TS SAADTV  ++H P+QS + K E S   S       ETM PS+S K+++VH
Sbjct: 31  EPTDEAETSNSAADTVPYMQHLPLQSHETKPELSPLESAQAPERSETMLPSKSHKKAKVH 90

Query: 61  SHSPSYSRAKNQPRIASKSPSLLKASP--TVASNKSPS---------------------- 120
           S   S+SRAK Q R A+K PS  K +P  +V+SNKSP+                      
Sbjct: 91  SQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSHDASKPSSSAGKVS 150

Query: 121 --------------------------SPASGKASPSKDASKPSSPAVAAPRPRISSVPPS 180
                                     SP +GKA PS+DAS+PSS A AAPR  I S PPS
Sbjct: 151 PSHDTSKLSSPAGKGKVSPSRDTSMPSPPAGKAFPSRDASQPSSAAAAAPRSHIRSKPPS 210

Query: 181 PSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASSTARSPSQANSKQQPSK--- 240
           PSQTSS+NHL+ K TS QS+LKADSQPSS SR AF PQASS  RSPS  NS+QQPSK   
Sbjct: 211 PSQTSSKNHLHSKQTS-QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKAS 270

Query: 241 ----------KTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKS 300
                     K TAQSTS Q T   A   DQTT  V SH A++S +AR K +E+Q QTKS
Sbjct: 271 RVQSPSHLSSKATAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRARCKSKENQVQTKS 330

Query: 301 KQSPKS-------KASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLE 360
           KQSPK        KASK+QP+  EE  SKNTS P SN++ SE P   D++IENG +PSLE
Sbjct: 331 KQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPLSNEDFSEIPIIIDETIENGPEPSLE 390

Query: 361 SQAESKEDEETK------EKATNA----SATSTLISSSESSSPYEEPEDRDSQKKELMGE 420
           SQ ES+E +E K      EK TNA    ++ S LI+S+E +SP+ EPE+ DSQ++  M  
Sbjct: 391 SQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPF-EPENSDSQQEGTM-- 450

Query: 421 GDISKAFPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQ 480
            D+ KAF  L I Y  EENPKSF TL GDNKG SM+L+SG+  +ESSIHI R+Y+S+PD+
Sbjct: 451 EDLPKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLISGEATKESSIHIHRQYKSDPDK 510

Query: 481 SPKSSTEIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKF 520
            P+SST+IEGN N +T +DS+T EDPPL LYIN N QGINNS+LSNSSFTE NPG +LKF
Sbjct: 511 VPESSTDIEGNSNEETPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKF 570

BLAST of MS016836 vs. NCBI nr
Match: XP_022951875.1 (cell wall protein RBR3-like [Cucurbita moschata])

HSP 1 Score: 441.0 bits (1133), Expect = 1.4e-119
Identity = 327/599 (54.59%), Postives = 392/599 (65.44%), Query Frame = 0

Query: 1   ENKEEAQTSASAADTVSTIRHQPVQSPKIKSEDSSSSSCETMPPSQSKKESQVHSHSPSY 60
           E  +EA+TS SAADTV  ++H P  SP ++S  +   S ETM PS+S K+++VHS   S+
Sbjct: 31  EPTDEAETSNSAADTVPYMQHLPELSP-LESAQAPERS-ETMLPSKSHKKAKVHSQPSSH 90

Query: 61  SRAKNQPRIASKSPSLLKASP--TVASNKSP----------------------------- 120
           SRAK Q R A+K PS  K +P  +V+SNKSP                             
Sbjct: 91  SRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTS 150

Query: 121 --SSPA-----------------SGKASPSKDASKPSSPAVAAPRPRISSVPPSPSQTSS 180
             SSPA                 +GKA PS+DAS+PSS A AAPR +I S PPSPSQTSS
Sbjct: 151 KLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSS 210

Query: 181 RNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASSTARSPSQANSKQQPSKKT------- 240
           +NH   KPTS QS+LKADSQPSS SR AF PQASS  RSPS  NS+QQPSKK        
Sbjct: 211 KNHPQSKPTS-QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPS 270

Query: 241 ------TAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKSKQSPKS 300
                 TAQSTS Q T   A   DQTT  V SH A++S +AR K RE+Q QTKSKQSPK 
Sbjct: 271 HLSSKPTAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRARCKRRENQVQTKSKQSPKP 330

Query: 301 -------KASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLESQAESK 360
                  KASK+QP+  EE  SKNTS P S+Q+ SE P   D++IENG + SLESQ ES+
Sbjct: 331 DLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIIIDETIENGPETSLESQTESQ 390

Query: 361 EDEETK------EKATNA----SATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKA 420
           E +E K      EK TNA    ++ S LI+S+E +SP+ EPE+ DSQ++  M   D+SKA
Sbjct: 391 ESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPF-EPENSDSQQEGTM--EDLSKA 450

Query: 421 FPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSST 480
           F  L I Y  EENPKSF TL GDNKG SM+LLSG+  +ESSIHI R+Y+S+PD+ P+SST
Sbjct: 451 FQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESSIHIHRQYKSDPDKGPESST 510

Query: 481 EIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTK 520
           +IEGN N +T +DS+T EDPPL LYIN N QGINNS+LSNSSFTE NPG +LKF  + TK
Sbjct: 511 DIEGNSNEETPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTK 570

BLAST of MS016836 vs. NCBI nr
Match: XP_023537866.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.0 bits (1107), Expect = 1.5e-116
Identity = 322/599 (53.76%), Postives = 388/599 (64.77%), Query Frame = 0

Query: 1   ENKEEAQTSASAADTVSTIRHQPVQSPKIKSEDSSSSSCETMPPSQSKKESQVHSHSPSY 60
           E  +EA+TS SAADTV  ++H P  SP ++S  +  +S ETM PS+S K+++VHS   S+
Sbjct: 31  EPTDEAETSNSAADTVPYMQHLPELSP-LESAQAPETS-ETMLPSKSHKKAKVHSQPSSH 90

Query: 61  SRAKNQPRIASKSPSLLKASP--TVASNKSP----------------------------- 120
           SRAK Q R A+K PS  K +P  +V+SNKSP                             
Sbjct: 91  SRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTS 150

Query: 121 --SSPA-----------------SGKASPSKDASKPSSPAVAAPRPRISSVPPSPSQTSS 180
             SSPA                 +GKA PS+DAS+PSS A AAPR +I S PPSPSQTSS
Sbjct: 151 KLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSS 210

Query: 181 RNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASSTARSPSQANSKQQPSKKT------- 240
           +NH   KPTS QS+LKADSQPSS SR A  PQASS  RSPS  NS+QQPSKK        
Sbjct: 211 KNHPQSKPTS-QSRLKADSQPSSPSRPALSPQASSIPRSPSHENSRQQPSKKASRVQSPS 270

Query: 241 ------TAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKSKQSPKS 300
                 TAQSTS Q T   A   DQTT  V SH A++S +AR K RE+Q QTKSKQSPK 
Sbjct: 271 HLSSKPTAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRARCKSRENQVQTKSKQSPKP 330

Query: 301 -------KASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLESQAESK 360
                  KASK+QP+  EE  SKNTS P S+Q+ SE P   D++IENG + SLESQ ES+
Sbjct: 331 DLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIIIDETIENGPETSLESQTESQ 390

Query: 361 EDEETK------EKATNA----SATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKA 420
           E +E K      EK TNA    ++ S LI+S+E +SP+ EPE+ DSQ++  M   D+SKA
Sbjct: 391 ESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPF-EPENSDSQQEGTM--EDLSKA 450

Query: 421 FPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSST 480
           F  L I Y  EENPKSF TL GDNKG SM+LLSG+  +ESSIHI R+Y+S+PD+ P+SST
Sbjct: 451 FQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESSIHIHRQYKSDPDKGPESST 510

Query: 481 EIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTK 520
           +IEGN N +T +DS+T EDPPL LYIN N QGINNS+LSNSSFTE NPG +L F  + TK
Sbjct: 511 DIEGNSNEETPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLNFVPQQTK 570

BLAST of MS016836 vs. NCBI nr
Match: KAG6585587.1 (hypothetical protein SDJN03_18320, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 430.6 bits (1106), Expect = 1.9e-116
Identity = 324/600 (54.00%), Postives = 391/600 (65.17%), Query Frame = 0

Query: 1   ENKEEAQTSASAADTVSTIRHQPVQSPKIKSEDSSSSSCETMPPSQSKKESQVHSHSPSY 60
           E  +EA+TS SAADTV  ++H P  SP ++S  +   S ETM PS+S K+++VHS   S+
Sbjct: 31  EPTDEAETSNSAADTVPYMQHPPELSP-LESAQAPERS-ETMLPSKSHKKAKVHSQPSSH 90

Query: 61  SRAKNQPRIASKSPSLLKASP--TVASNKSP----------------------------- 120
           SRAK Q R A+K PS  K +P  +V+SNKSP                             
Sbjct: 91  SRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTS 150

Query: 121 --SSPA-----------------SGKASPSKDASKPSSPAVAAPRPRISSVPPSPSQTSS 180
             SSPA                 +GKA PS+DAS+PSS A AAPR +I S PPSPSQTSS
Sbjct: 151 KLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSS 210

Query: 181 RNHLNPKPTSKQSKLKADS-QPSSSSRSAFRPQASSTARSPSQANSKQQPSKKT------ 240
           +NH   KPTS QS+LKADS QPSS S  AF PQASS  RSPS  NS+QQPSKK       
Sbjct: 211 KNHPQSKPTS-QSRLKADSPQPSSPSWPAFSPQASSIPRSPSHENSRQQPSKKASRVQSP 270

Query: 241 -------TAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKSKQSPK 300
                  TAQSTS Q T   A  EDQTT  V SH A++S  AR K +E+Q QTKSKQSPK
Sbjct: 271 SHLSSKPTAQSTSQQLTESPATIEDQTTKRVVSHPADQSPGARCKIKENQVQTKSKQSPK 330

Query: 301 S-------KASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLESQAES 360
                   KASK+QP+  EE  SKNTS P S+Q+ SE P   D++ ENG +PSLESQ ES
Sbjct: 331 PDLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIIIDETNENGPEPSLESQTES 390

Query: 361 KEDEETK------EKATNA----SATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISK 420
           +E +E K      EK TNA    ++ S LI+S+E +SP+ EPE+ DSQ++  M   D+SK
Sbjct: 391 QESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPF-EPENSDSQQEGTM--EDLSK 450

Query: 421 AFPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSS 480
           AF  L I Y  EENPKSF TL GDNKG SM+LLSG+  +ES+IHI R+Y+S+PD+ P+SS
Sbjct: 451 AFQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESAIHIHRQYKSDPDKVPESS 510

Query: 481 TEIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPT 520
           T+IEGN N +T +DS+T EDPPL LYIN N QGINNS+LSNSSFTE NPG +LKF  + T
Sbjct: 511 TDIEGNSNEETPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQT 570

BLAST of MS016836 vs. ExPASy TrEMBL
Match: A0A6J1CRH0 (cell wall protein RBR3 OS=Momordica charantia OX=3673 GN=LOC111013918 PE=4 SV=1)

HSP 1 Score: 869.4 bits (2245), Expect = 7.8e-249
Identity = 488/489 (99.80%), Postives = 489/489 (100.00%), Query Frame = 0

Query: 42  MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK 101
           MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK
Sbjct: 1   MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK 60

Query: 102 DASKPSSPAVAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRP 161
           DASKPSSPAVAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRP
Sbjct: 61  DASKPSSPAVAAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRP 120

Query: 162 QASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPK 221
           QASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPK
Sbjct: 121 QASSTARSPSQANSKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPK 180

Query: 222 GRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDP 281
           GRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDP
Sbjct: 181 GRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDP 240

Query: 282 SLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAF 341
           SLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAF
Sbjct: 241 SLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAF 300

Query: 342 PKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTE 401
           PKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTE
Sbjct: 301 PKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTE 360

Query: 402 IEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKS 461
           IEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKS
Sbjct: 361 IEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKS 420

Query: 462 EEISESLNTQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSN 521
           EEISESL+TQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSN
Sbjct: 421 EEISESLSTQKAKYIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSN 480

Query: 522 CKGKDGEIM 531
           CKGKDGEIM
Sbjct: 481 CKGKDGEIM 489

BLAST of MS016836 vs. ExPASy TrEMBL
Match: A0A6J1KJ10 (cell wall protein RBR3-like OS=Cucurbita maxima OX=3661 GN=LOC111496164 PE=4 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 4.0e-120
Identity = 324/605 (53.55%), Postives = 390/605 (64.46%), Query Frame = 0

Query: 1   ENKEEAQTSASAADTVSTIRHQPVQSPKIKSEDSSSSSC------ETMPPSQSKKESQVH 60
           E  +EA+TS SAADTV  ++H P+QS + K E S   S       ETM PS+S K+++VH
Sbjct: 31  EPTDEAETSNSAADTVPYMQHLPLQSHETKPELSPLESAQAPERSETMLPSKSHKKAKVH 90

Query: 61  SHSPSYSRAKNQPRIASKSPSLLKASP--TVASNKSPS---------------------- 120
           S   S+SRAK Q R A+K PS  K +P  +V+SNKSP+                      
Sbjct: 91  SQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSHDASKPSSSAGKVS 150

Query: 121 --------------------------SPASGKASPSKDASKPSSPAVAAPRPRISSVPPS 180
                                     SP +GKA PS+DAS+PSS A AAPR  I S PPS
Sbjct: 151 PSHDTSKLSSPAGKGKVSPSRDTSMPSPPAGKAFPSRDASQPSSAAAAAPRSHIRSKPPS 210

Query: 181 PSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASSTARSPSQANSKQQPSK--- 240
           PSQTSS+NHL+ K TS QS+LKADSQPSS SR AF PQASS  RSPS  NS+QQPSK   
Sbjct: 211 PSQTSSKNHLHSKQTS-QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKAS 270

Query: 241 ----------KTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKS 300
                     K TAQSTS Q T   A   DQTT  V SH A++S +AR K +E+Q QTKS
Sbjct: 271 RVQSPSHLSSKATAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRARCKSKENQVQTKS 330

Query: 301 KQSPKS-------KASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLE 360
           KQSPK        KASK+QP+  EE  SKNTS P SN++ SE P   D++IENG +PSLE
Sbjct: 331 KQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPLSNEDFSEIPIIIDETIENGPEPSLE 390

Query: 361 SQAESKEDEETK------EKATNA----SATSTLISSSESSSPYEEPEDRDSQKKELMGE 420
           SQ ES+E +E K      EK TNA    ++ S LI+S+E +SP+ EPE+ DSQ++  M  
Sbjct: 391 SQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPF-EPENSDSQQEGTM-- 450

Query: 421 GDISKAFPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQ 480
            D+ KAF  L I Y  EENPKSF TL GDNKG SM+L+SG+  +ESSIHI R+Y+S+PD+
Sbjct: 451 EDLPKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLISGEATKESSIHIHRQYKSDPDK 510

Query: 481 SPKSSTEIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKF 520
            P+SST+IEGN N +T +DS+T EDPPL LYIN N QGINNS+LSNSSFTE NPG +LKF
Sbjct: 511 VPESSTDIEGNSNEETPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKF 570

BLAST of MS016836 vs. ExPASy TrEMBL
Match: A0A6J1GK50 (cell wall protein RBR3-like OS=Cucurbita moschata OX=3662 GN=LOC111454611 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 6.8e-120
Identity = 327/599 (54.59%), Postives = 392/599 (65.44%), Query Frame = 0

Query: 1   ENKEEAQTSASAADTVSTIRHQPVQSPKIKSEDSSSSSCETMPPSQSKKESQVHSHSPSY 60
           E  +EA+TS SAADTV  ++H P  SP ++S  +   S ETM PS+S K+++VHS   S+
Sbjct: 31  EPTDEAETSNSAADTVPYMQHLPELSP-LESAQAPERS-ETMLPSKSHKKAKVHSQPSSH 90

Query: 61  SRAKNQPRIASKSPSLLKASP--TVASNKSP----------------------------- 120
           SRAK Q R A+K PS  K +P  +V+SNKSP                             
Sbjct: 91  SRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSHDASKPSSSAGKVSPSHDTS 150

Query: 121 --SSPA-----------------SGKASPSKDASKPSSPAVAAPRPRISSVPPSPSQTSS 180
             SSPA                 +GKA PS+DAS+PSS A AAPR +I S PPSPSQTSS
Sbjct: 151 KLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAAAAPRSQIRSKPPSPSQTSS 210

Query: 181 RNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASSTARSPSQANSKQQPSKKT------- 240
           +NH   KPTS QS+LKADSQPSS SR AF PQASS  RSPS  NS+QQPSKK        
Sbjct: 211 KNHPQSKPTS-QSRLKADSQPSSPSRPAFSPQASSIPRSPSHENSRQQPSKKASRVQSPS 270

Query: 241 ------TAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKSKQSPKS 300
                 TAQSTS Q T   A   DQTT  V SH A++S +AR K RE+Q QTKSKQSPK 
Sbjct: 271 HLSSKPTAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRARCKRRENQVQTKSKQSPKP 330

Query: 301 -------KASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLESQAESK 360
                  KASK+QP+  EE  SKNTS P S+Q+ SE P   D++IENG + SLESQ ES+
Sbjct: 331 DLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIIIDETIENGPETSLESQTESQ 390

Query: 361 EDEETK------EKATNA----SATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKA 420
           E +E K      EK TNA    ++ S LI+S+E +SP+ EPE+ DSQ++  M   D+SKA
Sbjct: 391 ESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPF-EPENSDSQQEGTM--EDLSKA 450

Query: 421 FPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSST 480
           F  L I Y  EENPKSF TL GDNKG SM+LLSG+  +ESSIHI R+Y+S+PD+ P+SST
Sbjct: 451 FQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESSIHIHRQYKSDPDKGPESST 510

Query: 481 EIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTK 520
           +IEGN N +T +DS+T EDPPL LYIN N QGINNS+LSNSSFTE NPG +LKF  + TK
Sbjct: 511 DIEGNSNEETPQDSKTEEDPPLELYININVQGINNSLLSNSSFTENNPGIKLKFVPQQTK 570

BLAST of MS016836 vs. ExPASy TrEMBL
Match: A0A5A7VAN0 (Flocculation protein FLO11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005610 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 1.4e-88
Identity = 311/763 (40.76%), Postives = 389/763 (50.98%), Query Frame = 0

Query: 4   EEAQTSASAADTVSTIRHQPVQSPKIKSED------SSSSSCETMPPSQSKKESQVHSHS 63
           +E+++SAS ADT   IRHQP QSP+IK E+       ++   ETMPPS+S KE ++HS  
Sbjct: 34  DESESSASQADTAPNIRHQPDQSPEIKPEEPPLATAQAAERSETMPPSKSHKEGKIHSQL 93

Query: 64  PSYSRAKNQPRIASKSPSLLKASP--TVASNKSPSSPASGKASPSKDASKPSSPA----- 123
            + SRAKN+ R ASK  S L A P   +ASNK PS+  SGK S S+D+SKPSSPA     
Sbjct: 94  STNSRAKNRSRTASKPSSPLNAIPQSPLASNKYPST--SGKGSKSQDSSKPSSPAGKVFS 153

Query: 124 --------------VAAPRPRISS-VPPSPSQTSSRNHLNPKPTSK-------------- 183
                         VAA  PRI+S    S SQ S++ H + KPTSK              
Sbjct: 154 PSQDASSKPSSPATVAATAPRIASKASSSSSQASNKKHPSSKPTSKLRFKADSQPSSPSR 213

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 214 SAFPSQDPSMPPQSLSQEKSRQQASEKSSRVQSPSHFSRKPTTQSTSKQPVESPATIGIQ 273

Query: 244 ---------QSKLKADSQPSSSSRSAF--------------------------------- 303
                    QS+ K DSQPS SSRS F                                 
Sbjct: 274 SHPNSKAPSQSRFKTDSQPSPSSRSTFPSQDFSMPPRSPSHENSRQQPSDKTSGVQSPSH 333

Query: 304 ---RPQASSTA------------------------------------------------- 363
              +P A ST+                                                 
Sbjct: 334 SSRKPTAQSTSKQPIESPATIGIQHHPNLKPSSQSRFKAESRPSSSSKSKFPSQDSSMPP 393

Query: 364 RSPSQANSKQQPSKKT-------------TAQSTSLQHTAFSAATEDQTTNGVTSHSANK 423
           RSPSQ NS Q PS+KT             TA STS Q    +A+  DQTT+G+ S  A  
Sbjct: 394 RSPSQENSLQPPSEKTSRVQSPSNLSRKPTAPSTSQQPIESTASIGDQTTDGILSDPATP 453

Query: 424 SSQARPKGRESQSQTKSKQSPKS-------KASKNQPQIKEELT----------SKNTSN 483
           S +A P   E Q Q KSK+SP+        KASKNQ   KEELT          SKNTSN
Sbjct: 454 SPKAIPTSGEIQIQAKSKKSPEPNVKPVELKASKNQNDTKEELTSKNETKEELASKNTSN 513

Query: 484 PQSNQNSSENPTRSDQSIENGQDPSLESQAESKEDEETKEKATNA----SATSTLISSSE 531
           P S+++SSENPT+SD+++E G D SLESQ ESKE +E   K TNA    ++ STLI+SS+
Sbjct: 514 PHSDEDSSENPTQSDETVERGLDSSLESQTESKETKEDGGKTTNALQAKASRSTLITSSK 573

BLAST of MS016836 vs. ExPASy TrEMBL
Match: A0A6J1K9X9 (probable serine/threonine-protein kinase samkC OS=Cucurbita maxima OX=3661 GN=LOC111492428 PE=4 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 3.8e-86
Identity = 256/537 (47.67%), Postives = 307/537 (57.17%), Query Frame = 0

Query: 4   EEAQTSASAADTVSTIRHQPVQSPKIKSED------SSSSSCETMPPSQSKKESQVHSHS 63
           +EA+TS+S ADTV  IRHQ VQSP+IK E             ETM P      + V S S
Sbjct: 24  DEAETSSSLADTVPNIRHQSVQSPEIKPEQPPLAPAQPPERTETM-PMPMPPPANVRSKS 83

Query: 64  PSYSRAKNQPRIASKSPSLLKASP--TVASNKSPSSPASGKASPSKDA-SKPSSPAVAAP 123
           PS SRAKNQ R  SK PS  K +P  +V SNKSP     GKASPS++A SKP SPA  AP
Sbjct: 84  PSQSRAKNQTRPVSKPPSPSKTTPQSSVDSNKSPL--VLGKASPSQEASSKPPSPAATAP 143

Query: 124 RPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASSTARSPSQAN 183
           R RI+S  PSPS  +S           QS+ KADSQP SSSRSAF PQ            
Sbjct: 144 RRRIASKSPSPSSQAS-----------QSRFKADSQPPSSSRSAFSPQ------------ 203

Query: 184 SKQQPSKKTTAQSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKSKQS 243
                        TSL+                                           
Sbjct: 204 -------------TSLE------------------------------------------- 263

Query: 244 PKSKASKNQPQIKEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLESQAESKEDEE 303
                  NQP+ KE+LTSKN SNP SN+NSS+ P +SDQ+IENG + S ESQ +SKE +E
Sbjct: 264 -------NQPETKEDLTSKNNSNPHSNKNSSKTPIQSDQTIENGLETSPESQEQSKETKE 323

Query: 304 TKEKATNASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAFPKLKINYSGEENP 363
             EK                          DS+++E M EG ++KAF KL I YS +ENP
Sbjct: 324 NLEK--------------------------DSKQEESM-EG-LAKAFQKLNIKYSDKENP 383

Query: 364 KSFITLIGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPKSSTEIEGNFNHKTTEDS 423
           KSF TLIG+NKG SM L SG+   +SSIHI R+Y++NP Q  KS TE+EGNF HKT +DS
Sbjct: 384 KSFTTLIGNNKGASMLLHSGEPKADSSIHIHRQYKNNPGQIAKSFTEMEGNFKHKTPQDS 434

Query: 424 RTVEDPPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKSEEISESLNTQKAK 483
           RT E+P  +LYIN N QG+NNSI+ +SSFTE +PG  LKF REPTKS++  +        
Sbjct: 444 RTEENPAQKLYININVQGMNNSIMLDSSFTENDPGINLKFPREPTKSKDELQ-------- 434

Query: 484 YIAKPAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYS-NCKGKDGEIM 531
            +AKPAER+T++P VRRRCLRGL MESSDSE +NPEKPRRHGCRYS  CKGK  E +
Sbjct: 504 -VAKPAERVTHEPAVRRRCLRGLLMESSDSEVDNPEKPRRHGCRYSRGCKGKKVETL 434

BLAST of MS016836 vs. TAIR 10
Match: AT1G75260.1 (oxidoreductases, acting on NADH or NADPH )

HSP 1 Score: 84.3 bits (207), Expect = 3.1e-16
Identity = 154/530 (29.06%), Postives = 236/530 (44.53%), Query Frame = 0

Query: 26  SPKIKSEDSSSSSCETMPPS----------QSKKESQVHSHSPSYSRAKNQ------PRI 85
           SP   S  +SS S    PPS          Q  K       SPS SR+++          
Sbjct: 15  SPSRISSGTSSPSPPPTPPSRPPFRPAGIAQPSKSETKPKASPSLSRSRSNVAALAASSS 74

Query: 86  ASKSPSLLKASPTVASNKSPSSPASGKASPSKDASKPSSPAVAAPRPRISSVPPSPSQTS 145
           AS+ PSL  A+PT  + +  ++  SG  S   D+ +     VA         PP  ++  
Sbjct: 75  ASQLPSLGAATPTRLAKQ--TNQQSGSPSKKLDSLRMEEQKVAT-----KEKPPGETEKI 134

Query: 146 SRNHLNPKPTSKQSKLKADSQPSSSSRSAFRPQASST--ARSPSQANSKQQP--SKKTTA 205
           +  +++P         + +         A + Q  +T  AR   Q N K  P  S K +A
Sbjct: 135 AEENISPVKEKPPIGARPEEHLEQKETEAVQEQGRNTEAARLVVQENKKVLPEGSGKKSA 194

Query: 206 QSTSLQHTAFSAATEDQTTNGVTSHSANKSSQARPKGRESQSQTKSKQSPKSKASKNQPQ 265
            +   Q          Q    V      +  +A       Q Q KSK++ K    + +  
Sbjct: 195 ANQGQQKIEEIEKIALQERKKVLHDDGVQKLEA------DQGQQKSKETEKLALQETKRS 254

Query: 266 I----KEELTSKNTSNPQSNQNSSENPTRSDQSIENGQDPSLESQAESKEDEETKEKATN 325
           +    +E+ T   T+    +  ++   TR  + +     P  +++ +++ +  T +   N
Sbjct: 255 LQAVGREDATRSKTTR---HMAAASETTRGPRDL-----PEKKTETQNRTEIPTDD---N 314

Query: 326 ASATSTLISSSESSSPYEEPEDRDSQKKELMGEGDISKAFPKLKINYS-GEENPKSFITL 385
              T   ++S+  +      E   S  +++  + DI     KL    S G++   S  TL
Sbjct: 315 HQKTKGALTSNLGNPRVTNREGSSSMSRKI--KEDIRDGISKLTWGKSNGDDKSVSVYTL 374

Query: 386 IGDNKGTSMNLLSGKGNRESSIHIRREYQSNPDQSPK-SSTEIEGNFNHKTTEDSRTVED 445
            G+NKG +M + S K  ++  +HIRR Y+SNPD+S   ++TE E        +D    E+
Sbjct: 375 TGENKGATMGIGSEKDKKDGEVHIRRGYRSNPDESSNTTATETE------NPKDDEAEEE 434

Query: 446 PPLRLYINNNTQGINNSILSNSSFTERNPGARLKFFREPTKSEEISESLNTQKAK-YIAK 505
                YIN NTQGINNSI+  SS +E +PG  + F  E  K E I    N ++ K     
Sbjct: 435 ASFTAYINGNTQGINNSIVVESSVSENDPGVHMSFKFEILKKEVIYPPENVEEKKPETVT 494

Query: 506 PAERLTYKPTVRRRCLRGLFMESSDSEPENPEKPRRHGCRYSNCKGKDGE 529
             ++L  +P VRRRCLRGL  ESS+SEP+NP KPRRHGCR++ CK KD E
Sbjct: 495 VTKKLKNEPRVRRRCLRGLLAESSESEPDNPLKPRRHGCRFT-CKDKDIE 511