MS016834 (gene) Bitter gourd (TR) v1

Overview


Name	MS016834
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA polymerase epsilon catalytic subunit A, putative
Location	scaffold9_1: 340972 .. 341619 (-)
RNA-Seq Expression	MS016834
Synteny	MS016834

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCTCTAATGGCCGGATGGAACTCTCCCGTCCATGATCCTAAATCAGGTATTTGAAATGAATTTCTGTTCGTATTTTAACCGATTCATGAACTATTCATCGCATTGTTGTTTGCTTATCGCGATGGATGTTGATCGTGTGTGTAATTACCGCGAATTTTCAGTCAAGTTCAAGAGGAATCGATCGCTTACCAAAGAGGAAATCGAAGCTTACTGGAGATCGAAGAGGAAGTTGGAGGAAGAGCATCTCAGAGCGATTTCTATGCCGGTGGATCCTCATGTTGATCGCGATGTGGTCGGAATAGATTACAAAAGATCGAGTTCGTTTCCGCCTGCGAACGGCACGGCGGAAGAAGTATTTGTGAATCGGAGCGATTTCGAGAGGAAAAACCTAGAGAAATTGAAGCCAAATGACTGGTAAATTTAAGTCGTGTACCACTCGATCGTCGATTTTAGTGTTTGAAATTCAGGAACGAAATTAATAATAATAATTAAACCAGAGGTCATGTGCGGTTTAATTTCTGTTTTTTGTGCAGGTGGATGAGGAGCAACTCGGCTTTTCTGAATGAACCGCCGGTCCTCGACGGCGCTGCCAAAGCTTACGCGGCCTCGTTCAAGTTGAATAACAGTAACGGAATCGGTGCT

mRNA sequence

ATGGGTTCTCTAATGGCCGGATGGAACTCTCCCGTCCATGATCCTAAATCAGTCAAGTTCAAGAGGAATCGATCGCTTACCAAAGAGGAAATCGAAGCTTACTGGAGATCGAAGAGGAAGTTGGAGGAAGAGCATCTCAGAGCGATTTCTATGCCGGTGGATCCTCATGTTGATCGCGATGTGGTCGGAATAGATTACAAAAGATCGAGTTCGTTTCCGCCTGCGAACGGCACGGCGGAAGAAGTATTTGTGAATCGGAGCGATTTCGAGAGGAAAAACCTAGAGAAATTGAAGCCAAATGACTGGTGGATGAGGAGCAACTCGGCTTTTCTGAATGAACCGCCGGTCCTCGACGGCGCTGCCAAAGCTTACGCGGCCTCGTTCAAGTTGAATAACAGTAACGGAATCGGTGCT

Coding sequence (CDS)

ATGGGTTCTCTAATGGCCGGATGGAACTCTCCCGTCCATGATCCTAAATCAGTCAAGTTCAAGAGGAATCGATCGCTTACCAAAGAGGAAATCGAAGCTTACTGGAGATCGAAGAGGAAGTTGGAGGAAGAGCATCTCAGAGCGATTTCTATGCCGGTGGATCCTCATGTTGATCGCGATGTGGTCGGAATAGATTACAAAAGATCGAGTTCGTTTCCGCCTGCGAACGGCACGGCGGAAGAAGTATTTGTGAATCGGAGCGATTTCGAGAGGAAAAACCTAGAGAAATTGAAGCCAAATGACTGGTGGATGAGGAGCAACTCGGCTTTTCTGAATGAACCGCCGGTCCTCGACGGCGCTGCCAAAGCTTACGCGGCCTCGTTCAAGTTGAATAACAGTAACGGAATCGGTGCT

Protein sequence

MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRDVVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGAAKAYAASFKLNNSNGIGA

Homology

[Close]

BLAST of MS016834 vs. NCBI nr
Match: XP_022144322.1 (uncharacterized protein LOC111014033 [Momordica charantia])

HSP 1 Score: 277.3 bits (708), Expect = 7.0e-71
Identity = 135/138 (97.83%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLMAGWNSPV DPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD
Sbjct: 1   MGSLMAGWNSPVQDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
           VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWW RSNSAFLNEPPVLDGA
Sbjct: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWTRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASFKLNNSNGIGA 139
           AKAYAASFKLN+SNGIGA
Sbjct: 121 AKAYAASFKLNHSNGIGA 138

[Close]

BLAST of MS016834 vs. NCBI nr
Match: XP_038886573.1 (uncharacterized protein LOC120076743 [Benincasa hispida])

HSP 1 Score: 208.4 bits (529), Expect = 4.0e-50
Identity = 104/146 (71.23%), Postives = 117/146 (80.14%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM+GWNSPV DPKSVKFKRNRSLTK+EIEAYW+SK+K EEEHLRAISMPVD ++DR+
Sbjct: 1   MGSLMSGWNSPVLDPKSVKFKRNRSLTKDEIEAYWKSKKKSEEEHLRAISMPVDRYIDRE 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFPPA   + E     +D ER+NLEKLK   WW RSNSAFLNEPPVLDGA
Sbjct: 61  TVGIDYKRSSSFPPAKDVSAEAEFAENDLERENLEKLKKIGWWTRSNSAFLNEPPVLDGA 120

Query: 121 AKAYA--------ASFKLNNSNGIGA 139
            K YA        AS KLN+SNG+G+
Sbjct: 121 TKGYASLYGVADVASCKLNHSNGVGS 146

[Close]

BLAST of MS016834 vs. NCBI nr
Match: XP_023002545.1 (uncharacterized protein LOC111496358 [Cucurbita maxima])

HSP 1 Score: 206.1 bits (523), Expect = 2.0e-49
Identity = 104/128 (81.25%), Postives = 108/128 (84.38%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GWNSPV DPKSVKFKRNRSLTKEEIEAYW+SKRKLEEEHLRAISMP    VDRD
Sbjct: 1   MGSLMGGWNSPVLDPKSVKFKRNRSLTKEEIEAYWKSKRKLEEEHLRAISMP----VDRD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFP A G AE+     SD ERKNLEKLK N WW RSNSAFLNEPPVLDGA
Sbjct: 61  TVGIDYKRSSSFPAAIGAAEKEVFAESDLERKNLEKLKKNGWWRRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASF 129
           AKAYA+ +
Sbjct: 121 AKAYASQY 124

[Close]

BLAST of MS016834 vs. NCBI nr
Match: KAG6585589.1 (hypothetical protein SDJN03_18322, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020504.1 hypothetical protein SDJN02_17189, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 205.7 bits (522), Expect = 2.6e-49
Identity = 104/128 (81.25%), Postives = 108/128 (84.38%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GWNSPV DPKSVKFKRNRSLTKEEIEAYW+SKRKLEEEHLRAISMP    VDRD
Sbjct: 1   MGSLMGGWNSPVLDPKSVKFKRNRSLTKEEIEAYWKSKRKLEEEHLRAISMP----VDRD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFP A G AE+     SD ERKNLEKLK N WW RSNSAFLNEPPVLDGA
Sbjct: 61  TVGIDYKRSSSFPAAIGKAEKEVFAESDLERKNLEKLKKNGWWRRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASF 129
           AKAYA+ +
Sbjct: 121 AKAYASQY 124

[Close]

BLAST of MS016834 vs. NCBI nr
Match: XP_023536775.1 (uncharacterized protein LOC111798054 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.8 bits (517), Expect = 9.9e-49
Identity = 102/128 (79.69%), Postives = 107/128 (83.59%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GWNSPV DPKSVKFKRNRSLTKEEIEAYW+SKRKLEEEHLRA+SMP    VDRD
Sbjct: 1   MGSLMGGWNSPVLDPKSVKFKRNRSLTKEEIEAYWKSKRKLEEEHLRAVSMP----VDRD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFP A G AE+     SD ERKNLE LK N WW RSNSAFLNEPPVLDGA
Sbjct: 61  TVGIDYKRSSSFPAAIGKAEKEVFTESDLERKNLEMLKKNGWWRRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASF 129
           AKAYA+ +
Sbjct: 121 AKAYASQY 124

[Close]

BLAST of MS016834 vs. ExPASy TrEMBL
Match: A0A6J1CTD0 (uncharacterized protein LOC111014033 OS=Momordica charantia OX=3673 GN=LOC111014033 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.4e-71
Identity = 135/138 (97.83%), Postives = 136/138 (98.55%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLMAGWNSPV DPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD
Sbjct: 1   MGSLMAGWNSPVQDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
           VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWW RSNSAFLNEPPVLDGA
Sbjct: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWTRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASFKLNNSNGIGA 139
           AKAYAASFKLN+SNGIGA
Sbjct: 121 AKAYAASFKLNHSNGIGA 138

[Close]

BLAST of MS016834 vs. ExPASy TrEMBL
Match: A0A6J1KP90 (uncharacterized protein LOC111496358 OS=Cucurbita maxima OX=3661 GN=LOC111496358 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 9.6e-50
Identity = 104/128 (81.25%), Postives = 108/128 (84.38%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GWNSPV DPKSVKFKRNRSLTKEEIEAYW+SKRKLEEEHLRAISMP    VDRD
Sbjct: 1   MGSLMGGWNSPVLDPKSVKFKRNRSLTKEEIEAYWKSKRKLEEEHLRAISMP----VDRD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFP A G AE+     SD ERKNLEKLK N WW RSNSAFLNEPPVLDGA
Sbjct: 61  TVGIDYKRSSSFPAAIGAAEKEVFAESDLERKNLEKLKKNGWWRRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASF 129
           AKAYA+ +
Sbjct: 121 AKAYASQY 124

[Close]

BLAST of MS016834 vs. ExPASy TrEMBL
Match: A0A6J1GI71 (uncharacterized protein LOC111454424 OS=Cucurbita moschata OX=3662 GN=LOC111454424 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 8.2e-49
Identity = 103/128 (80.47%), Postives = 107/128 (83.59%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GWNSPV DPKSVKFKRNRSLTKEEIEAYW+SKRKLEEEHLRAISMP    VD D
Sbjct: 1   MGSLMGGWNSPVLDPKSVKFKRNRSLTKEEIEAYWKSKRKLEEEHLRAISMP----VDGD 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFP A G AE+     SD ERKNLEKLK N WW RSNSAFLNEPPVLDGA
Sbjct: 61  TVGIDYKRSSSFPAAIGKAEKEVFAESDLERKNLEKLKKNGWWRRSNSAFLNEPPVLDGA 120

Query: 121 AKAYAASF 129
           AKAYA+ +
Sbjct: 121 AKAYASQY 124

[Close]

BLAST of MS016834 vs. ExPASy TrEMBL
Match: A0A0A0LRI3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361750 PE=4 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.8e-46
Identity = 101/147 (68.71%), Postives = 114/147 (77.55%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GWNSPV DPKSVKFKRNRSLTKEEIEAYW+SKRKLE+EHLRA+S+PVD   D +
Sbjct: 1   MGSLMGGWNSPVLDPKSVKFKRNRSLTKEEIEAYWKSKRKLEKEHLRAVSLPVDDRYDCE 60

Query: 61  VVGIDYKRSSSFPPA-NGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDG 120
            VG+DYKRSSSFPPA +  A E     +  +RKNLE+LK   WW RSNSAFLNEPPVLDG
Sbjct: 61  TVGLDYKRSSSFPPAKDDVAVEAKFVENKMDRKNLEELKKIGWWTRSNSAFLNEPPVLDG 120

Query: 121 AAKAY--------AASFKLNNSNGIGA 139
           A+KA          AS KLN+SNG+GA
Sbjct: 121 ASKACVSLYGVADVASCKLNHSNGVGA 147

[Close]

BLAST of MS016834 vs. ExPASy TrEMBL
Match: A0A5A7VD66 (Putative DNA polymerase epsilon catalytic subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005630 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 8.4e-46
Identity = 101/146 (69.18%), Postives = 115/146 (78.77%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRKLEEEHLRAISMPVDPHVDRD 60
           MGSLM GW+SPV DPKSVKFKRNRSLTKEEI+AYW+SKRKLEEEHLRAIS+PVD + DR+
Sbjct: 1   MGSLMGGWSSPVLDPKSVKFKRNRSLTKEEIDAYWKSKRKLEEEHLRAISLPVDRY-DRE 60

Query: 61  VVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDGA 120
            VGIDYKRSSSFPPA   A E     +  +RKNLE+L+   WW RSNSAFLNEPPVLDGA
Sbjct: 61  KVGIDYKRSSSFPPARDVAVESKFVENKLDRKNLEELEKVGWWTRSNSAFLNEPPVLDGA 120

Query: 121 AKAY--------AASFKLNNSNGIGA 139
           +KA          AS KLN++NG+GA
Sbjct: 121 SKACVSLYGVADVASCKLNHNNGVGA 145

[Close]

BLAST of MS016834 vs. TAIR 10
Match: AT1G19530.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation, anaerobic respiration; LOCATED IN: cellular_component unknown; EXPRESSED IN: leaf apex, inflorescence meristem, hypocotyl, root, flower; EXPRESSED DURING: petal differentiation and expansion stage; Has 47 Blast hits to 47 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 47; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 77.8 bits (190), Expect = 7.6e-15
Identity = 48/131 (36.64%), Postives = 73/131 (55.73%), Query Frame = 0


Query: 1   MGSLMAGWNSPVHDPKSVKFKRNRSLTKEEIEAYWRSKRK-LEEEHLRAISMPVDPHVDR 60
           MGSLM+GW+S V DPKSV  +R +SLT+EEI+ +W++K+K  EEEH++A S  V      
Sbjct: 1   MGSLMSGWDSRVRDPKSV--RRCKSLTREEIDTFWKTKKKNEEEEHVQAFSKLV------ 60

Query: 61  DVVGIDYKRSSSFPPANGTAEEVFVNRSDFERKNLEKLKPNDWWMRSNSAFLNEPPVLDG 120
                  + + S      + +++F N+S          K + WW ++  AFLNEP   +G
Sbjct: 61  -----TQEGAQSQAKEKKSVDDLFENQS----------KSSGWWRKTYWAFLNEPREEEG 108

Query: 121 AAKAYAASFKL 131
               Y + FK+
Sbjct: 121 RPNNYVSQFKV 108

The following BLAST results are available for this feature:

BLAST of MS016834 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144322.1	7.0e-71	97.83	uncharacterized protein LOC111014033 [Momordica charantia]	[more]
XP_038886573.1	4.0e-50	71.23	uncharacterized protein LOC120076743 [Benincasa hispida]	[more]
XP_023002545.1	2.0e-49	81.25	uncharacterized protein LOC111496358 [Cucurbita maxima]	[more]
KAG6585589.1	2.6e-49	81.25	hypothetical protein SDJN03_18322, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023536775.1	9.9e-49	79.69	uncharacterized protein LOC111798054 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS016834 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS016834 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTD0	3.4e-71	97.83	uncharacterized protein LOC111014033 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1KP90	9.6e-50	81.25	uncharacterized protein LOC111496358 OS=Cucurbita maxima OX=3661 GN=LOC111496358...	[more]
A0A6J1GI71	8.2e-49	80.47	uncharacterized protein LOC111454424 OS=Cucurbita moschata OX=3662 GN=LOC1114544...	[more]
A0A0A0LRI3	3.8e-46	68.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361750 PE=4 SV=1	[more]
A0A5A7VD66	8.4e-46	69.18	Putative DNA polymerase epsilon catalytic subunit A OS=Cucumis melo var. makuwa ...	[more]

BLAST of MS016834 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19530.1	7.6e-15	36.64	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33872	DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A	coord: 1..132
None	No IPR available	PANTHER	PTHR33872:SF2	DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A	coord: 1..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016834.1	MS016834.1	mRNA