MS016823 (gene) Bitter gourd (TR) v1

Overview


Name	MS016823
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	B3 domain-containing protein At2g31420-like
Location	scaffold9_1: 262587 .. 263018 (+)
RNA-Seq Expression	MS016823
Synteny	MS016823

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAGCACCGCTAAGGGATCGCATCGCTGAACTTGGCGGCTACGACATCCAAATAGTGATCCAAAAGCGATTGCAGGACACTGACCTTAATCGGAACCATGGTCGATTGTCAATTCCGATTAAGAAGCTCCTTAATGATTTCGCTACAGAGGAGGAGAAGAAGGTGTTGGACCAACAAGACGAACATCAAAAGAACAAAAGTGGAGTGAGCGCGCTGATTCTTGATCCTTGTTTGCGAGAGAGCGTCATTCATGTGAAGAAGTGGAAGATCGGAAGCCGCGAAATCTACTGCTTGGTGTCTCAGTGGAACGCTTTGGCGCAACAAAATTCCTTGGAAAGTGGAGACTACATCCAACTTTGGAGTTTTAGAAAGAATAATGGAACATCCCCACCTCAACTTTGCTTCGCATTGGTCAAGCTCGACATATCT

mRNA sequence

ATGCCAGCACCGCTAAGGGATCGCATCGCTGAACTTGGCGGCTACGACATCCAAATAGTGATCCAAAAGCGATTGCAGGACACTGACCTTAATCGGAACCATGGTCGATTGTCAATTCCGATTAAGAAGCTCCTTAATGATTTCGCTACAGAGGAGGAGAAGAAGGTGTTGGACCAACAAGACGAACATCAAAAGAACAAAAGTGGAGTGAGCGCGCTGATTCTTGATCCTTGTTTGCGAGAGAGCGTCATTCATGTGAAGAAGTGGAAGATCGGAAGCCGCGAAATCTACTGCTTGGTGTCTCAGTGGAACGCTTTGGCGCAACAAAATTCCTTGGAAAGTGGAGACTACATCCAACTTTGGAGTTTTAGAAAGAATAATGGAACATCCCCACCTCAACTTTGCTTCGCATTGGTCAAGCTCGACATATCT

Coding sequence (CDS)

ATGCCAGCACCGCTAAGGGATCGCATCGCTGAACTTGGCGGCTACGACATCCAAATAGTGATCCAAAAGCGATTGCAGGACACTGACCTTAATCGGAACCATGGTCGATTGTCAATTCCGATTAAGAAGCTCCTTAATGATTTCGCTACAGAGGAGGAGAAGAAGGTGTTGGACCAACAAGACGAACATCAAAAGAACAAAAGTGGAGTGAGCGCGCTGATTCTTGATCCTTGTTTGCGAGAGAGCGTCATTCATGTGAAGAAGTGGAAGATCGGAAGCCGCGAAATCTACTGCTTGGTGTCTCAGTGGAACGCTTTGGCGCAACAAAATTCCTTGGAAAGTGGAGACTACATCCAACTTTGGAGTTTTAGAAAGAATAATGGAACATCCCCACCTCAACTTTGCTTCGCATTGGTCAAGCTCGACATATCT

Protein sequence

MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQDEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQLWSFRKNNGTSPPQLCFALVKLDIS

Homology

[Close]

BLAST of MS016823 vs. NCBI nr
Match: XP_022144152.1 (B3 domain-containing protein At2g31420-like [Momordica charantia])

HSP 1 Score: 267.7 bits (683), Expect = 5.8e-68
Identity = 128/144 (88.89%), Postives = 136/144 (94.44%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MPA LRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDF TEEEK++LDQQ
Sbjct: 52  MPAXLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFTTEEEKQLLDQQ 111

Query: 61  DEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQL 120
           DEHQ NK+GV ALILDPCLRES IH+KKWKIGSREIYCLVSQWN+LAQ+NSLESGD+IQL
Sbjct: 112 DEHQNNKNGVXALILDPCLRESTIHLKKWKIGSREIYCLVSQWNSLAQKNSLESGDFIQL 171

Query: 121 WSFRKNNGTSPPQLCFALVKLDIS 145
           WSFRKN+GT  P LCFALVKLDIS
Sbjct: 172 WSFRKNSGTPTPHLCFALVKLDIS 195

[Close]

BLAST of MS016823 vs. NCBI nr
Match: XP_022144153.1 (uncharacterized protein LOC111013910 [Momordica charantia])

HSP 1 Score: 222.6 bits (566), Expect = 2.1e-54
Identity = 110/113 (97.35%), Postives = 110/113 (97.35%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MPA LRDRIAELGGYDIQ VIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ
Sbjct: 49  MPALLRDRIAELGGYDIQXVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 108

Query: 61  DEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLE 114
           DEHQKNKSGVSALILDPCLRES IHVKKWKIGSREIYCLVSQWNALAQQNSLE
Sbjct: 109 DEHQKNKSGVSALILDPCLRESAIHVKKWKIGSREIYCLVSQWNALAQQNSLE 161

[Close]

BLAST of MS016823 vs. NCBI nr
Match: XP_008445565.1 (PREDICTED: B3 domain-containing protein At3g25182-like [Cucumis melo] >KAA0065235.1 B3 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 186.8 bits (473), Expect = 1.3e-43
Identity = 96/154 (62.34%), Postives = 114/154 (74.03%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           M A LRDRI EL G+DIQ V QKRLQDTD+N+NHGRL IP KKL NDFA+EEEK++L Q 
Sbjct: 93  MSAMLRDRIDELDGFDIQYVTQKRLQDTDMNKNHGRLLIPFKKLRNDFASEEEKQLLKQH 152

Query: 61  D-EHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQ 120
           D E  KNK+G++ LI+DP LR   I +K WK+G  + YCL+S+WN+  Q+NSLESGDYIQ
Sbjct: 153 DQEDLKNKNGMTVLIIDPQLRNRHIELKMWKVGQHDSYCLMSKWNSFVQENSLESGDYIQ 212

Query: 121 LWSFRKNN------GTSPP---QLCFALVKLDIS 145
           LWSFR NN       +  P    LCFALVKLDIS
Sbjct: 213 LWSFRSNNIFIVGHQSQVPVNSNLCFALVKLDIS 246

[Close]

BLAST of MS016823 vs. NCBI nr
Match: XP_004152777.2 (putative B3 domain-containing protein At2g27410 [Cucumis sativus] >KAE8652107.1 hypothetical protein Csa_022429 [Cucumis sativus])

HSP 1 Score: 184.9 bits (468), Expect = 5.0e-43
Identity = 92/154 (59.74%), Postives = 111/154 (72.08%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MP  LRDRI ELGG++IQ   QKRLQDTD+N+NHGRL IP KKL+NDFA+EEEK++L Q 
Sbjct: 98  MPQMLRDRIEELGGFNIQFATQKRLQDTDMNKNHGRLLIPFKKLVNDFASEEEKRLLKQH 157

Query: 61  D-EHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQ 120
           D E  KNK+G++ LI+DP LR   I +K WK+G  E YCL+S+WN   ++NSLESGDYIQ
Sbjct: 158 DQEDLKNKNGMTVLIIDPQLRNRQIGLKMWKVGQHESYCLMSKWNLFVKENSLESGDYIQ 217

Query: 121 LWSFRKNNG---------TSPPQLCFALVKLDIS 145
           LWSFR +N               LCFA VKLDIS
Sbjct: 218 LWSFRSDNAFIVGHEAQLPINSNLCFAFVKLDIS 251

[Close]

BLAST of MS016823 vs. NCBI nr
Match: KAG6598443.1 (B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 183.3 bits (464), Expect = 1.4e-42
Identity = 88/147 (59.86%), Postives = 112/147 (76.19%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MPA LR+RI  +GG D+Q+V QK+LQ++D N+NHGRL IP KKL+NDFATEEE +++ Q+
Sbjct: 49  MPAALRERIEAMGGTDVQLVTQKQLQESDRNKNHGRLLIPTKKLMNDFATEEEAELMMQE 108

Query: 61  DEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQL 120
           +  ++ + G+   I+D  LRESVI +K WKIGS   YCL S+WN+ A+QNSLESGDYIQL
Sbjct: 109 EVDRRRRIGMQVAIIDSALRESVIGLKLWKIGSGHNYCLASRWNSFAEQNSLESGDYIQL 168

Query: 121 WSFRKNNG---TSPPQLCFALVKLDIS 145
           WSFR NN     +   LCFALVKLDIS
Sbjct: 169 WSFRSNNNAFIVNDHSLCFALVKLDIS 195

[Close]

BLAST of MS016823 vs. ExPASy Swiss-Prot
Match: Q5RM09 (B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05920 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 8.6e-14
Identity = 44/133 (33.08%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 13  GGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVS 72
           G  D +++I+K L   D++    RLSIPI  ++ NDF T +E +++D+ +   +   GV+
Sbjct: 176 GAGDPKLIIEKNLDSNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDEDEITNEGNMGVA 235

Query: 73  ALILDPCLRESVIHVKKWKI-----GSREIYCLVSQWNALAQQNSLESGDYIQLWSFRKN 132
           A ++D   ++  +  K+W +      S   + L  +W+ + + N L+ GD I LWSFR N
Sbjct: 236 AFLVDQRTKKWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRSN 295

Query: 133 NGTSPPQLCFALV 140
           +      LCFALV
Sbjct: 296 D-----ILCFALV 303

[Close]

BLAST of MS016823 vs. ExPASy Swiss-Prot
Match: Q9LSF7 (B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25182 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-13
Identity = 45/129 (34.88%), Postives = 72/129 (55.81%), Query Frame = 0


Query: 16  DIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVSALI 75
           D +++ +K L  TD+N    RLS+P   LL NDF T  E +++D+  ++ K K GV A++
Sbjct: 212 DAKLIFEKTLFVTDVNPTQNRLSMPFNNLLRNDFLTSVESRIIDKDIKNDK-KIGVGAIL 271

Query: 76  LDPCLRESVIHVKKWKIGSRE-----IYCLVSQWNALAQQNSLESGDYIQLWSFRKNNGT 135
           +D   ++  + +K+W++          Y L+  WN + + N L+ GD I LWSFR     
Sbjct: 272 VDQRCKKWGVMLKRWEMKKESGKGSWNYNLICGWNDIVEANGLKEGDNISLWSFR----- 331

Query: 136 SPPQLCFAL 139
           S   LCFA+
Sbjct: 332 SCGILCFAM 334

[Close]

BLAST of MS016823 vs. ExPASy Swiss-Prot
Match: Q9XIP5 (Putative B3 domain-containing protein At2g27410 OS=Arabidopsis thaliana OX=3702 GN=At2g27410 PE=3 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 5.6e-13
Identity = 45/131 (34.35%), Postives = 72/131 (54.96%), Query Frame = 0


Query: 11  ELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLN-DFATEEEKKVLDQQDEHQKNKSG 70
           E  GY+ +++  ++L  TDL +   RLS+P K++   DF TE+E +++  ++  +   +G
Sbjct: 80  EENGYNPKLISTRQLYKTDLKKTEARLSVPFKQVKTPDFLTEDETRII-HENAMKIRDNG 139

Query: 71  VSALILDPCLRESVIHVKKWKIGSREIYCLVSQW-NAL-AQQNSLESGDYIQLWSFRKNN 130
           V    +DP L + V+ ++KWK+    IY  V  W N L A +   +  D   LWSFR   
Sbjct: 140 VPVNFVDPELNKHVLELRKWKMKGNWIYVFVKGWKNVLDACKTLFKEDDVYPLWSFRSGT 199

Query: 131 GTSPPQLCFAL 139
           G    +LCFAL
Sbjct: 200 G----KLCFAL 205

[Close]

BLAST of MS016823 vs. ExPASy Swiss-Prot
Match: Q9FLV7 (B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24050 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 4.7e-12
Identity = 40/115 (34.78%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 16  DIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVSALI 75
           D +++ +K L  TD+N    RLS+P   LL NDF T  E  +++ +D +  NK GV A++
Sbjct: 215 DAKLIFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIIIN-EDINNNNKIGVGAIL 274

Query: 76  LDPCLRESVIHVKKWKIGSRE-----IYCLVSQWNALAQQNSLESGDYIQLWSFR 125
           +D   ++  + +K+W++          Y L+  WN + + N L+ GD I LWSFR
Sbjct: 275 VDQRCKKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLKEGDNITLWSFR 328

[Close]

BLAST of MS016823 vs. ExPASy Swiss-Prot
Match: Q9LRX6 (Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 GN=At3g24850 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 8.0e-12
Identity = 42/129 (32.56%), Postives = 69/129 (53.49%), Query Frame = 0


Query: 16  DIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVSALI 75
           D +++ +K L  TD+N    RLS+P   LL NDF T  E +++ ++D +   K GV A++
Sbjct: 225 DAKLIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRII-KEDINNNKKIGVGAIL 284

Query: 76  LDPCLRESVIHVKKWKIGSRE-----IYCLVSQWNALAQQNSLESGDYIQLWSFRKNNGT 135
           +D    +  + +K+W++          Y L+  WN + + N L+ GD I +WSFR     
Sbjct: 285 VDQRSVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRCRG-- 344

Query: 136 SPPQLCFAL 139
               LCFA+
Sbjct: 345 ---VLCFAM 347

[Close]

BLAST of MS016823 vs. ExPASy TrEMBL
Match: A0A6J1CSV9 (B3 domain-containing protein At2g31420-like OS=Momordica charantia OX=3673 GN=LOC111013909 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.8e-68
Identity = 128/144 (88.89%), Postives = 136/144 (94.44%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MPA LRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDF TEEEK++LDQQ
Sbjct: 52  MPAXLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFTTEEEKQLLDQQ 111

Query: 61  DEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQL 120
           DEHQ NK+GV ALILDPCLRES IH+KKWKIGSREIYCLVSQWN+LAQ+NSLESGD+IQL
Sbjct: 112 DEHQNNKNGVXALILDPCLRESTIHLKKWKIGSREIYCLVSQWNSLAQKNSLESGDFIQL 171

Query: 121 WSFRKNNGTSPPQLCFALVKLDIS 145
           WSFRKN+GT  P LCFALVKLDIS
Sbjct: 172 WSFRKNSGTPTPHLCFALVKLDIS 195

[Close]

BLAST of MS016823 vs. ExPASy TrEMBL
Match: A0A6J1CSJ5 (uncharacterized protein LOC111013910 OS=Momordica charantia OX=3673 GN=LOC111013910 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.0e-54
Identity = 110/113 (97.35%), Postives = 110/113 (97.35%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MPA LRDRIAELGGYDIQ VIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ
Sbjct: 49  MPALLRDRIAELGGYDIQXVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 108

Query: 61  DEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLE 114
           DEHQKNKSGVSALILDPCLRES IHVKKWKIGSREIYCLVSQWNALAQQNSLE
Sbjct: 109 DEHQKNKSGVSALILDPCLRESAIHVKKWKIGSREIYCLVSQWNALAQQNSLE 161

[Close]

BLAST of MS016823 vs. ExPASy TrEMBL
Match: A0A5A7VHB3 (B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005760 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 6.3e-44
Identity = 96/154 (62.34%), Postives = 114/154 (74.03%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           M A LRDRI EL G+DIQ V QKRLQDTD+N+NHGRL IP KKL NDFA+EEEK++L Q 
Sbjct: 93  MSAMLRDRIDELDGFDIQYVTQKRLQDTDMNKNHGRLLIPFKKLRNDFASEEEKQLLKQH 152

Query: 61  D-EHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQ 120
           D E  KNK+G++ LI+DP LR   I +K WK+G  + YCL+S+WN+  Q+NSLESGDYIQ
Sbjct: 153 DQEDLKNKNGMTVLIIDPQLRNRHIELKMWKVGQHDSYCLMSKWNSFVQENSLESGDYIQ 212

Query: 121 LWSFRKNN------GTSPP---QLCFALVKLDIS 145
           LWSFR NN       +  P    LCFALVKLDIS
Sbjct: 213 LWSFRSNNIFIVGHQSQVPVNSNLCFALVKLDIS 246

[Close]

BLAST of MS016823 vs. ExPASy TrEMBL
Match: A0A1S3BCJ5 (B3 domain-containing protein At3g25182-like OS=Cucumis melo OX=3656 GN=LOC103488545 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 6.3e-44
Identity = 96/154 (62.34%), Postives = 114/154 (74.03%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           M A LRDRI EL G+DIQ V QKRLQDTD+N+NHGRL IP KKL NDFA+EEEK++L Q 
Sbjct: 93  MSAMLRDRIDELDGFDIQYVTQKRLQDTDMNKNHGRLLIPFKKLRNDFASEEEKQLLKQH 152

Query: 61  D-EHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQ 120
           D E  KNK+G++ LI+DP LR   I +K WK+G  + YCL+S+WN+  Q+NSLESGDYIQ
Sbjct: 153 DQEDLKNKNGMTVLIIDPQLRNRHIELKMWKVGQHDSYCLMSKWNSFVQENSLESGDYIQ 212

Query: 121 LWSFRKNN------GTSPP---QLCFALVKLDIS 145
           LWSFR NN       +  P    LCFALVKLDIS
Sbjct: 213 LWSFRSNNIFIVGHQSQVPVNSNLCFALVKLDIS 246

[Close]

BLAST of MS016823 vs. ExPASy TrEMBL
Match: A0A6J1H5Z0 (putative B3 domain-containing protein At3g24850 OS=Cucurbita moschata OX=3662 GN=LOC111460418 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.7e-42
Identity = 87/147 (59.18%), Postives = 112/147 (76.19%), Query Frame = 0


Query: 1   MPAPLRDRIAELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLNDFATEEEKKVLDQQ 60
           MPA +RDRI E+G Y IQ+ IQK+LQDTD+N+NHGRLS P KKL  DFATEEE++VL QQ
Sbjct: 112 MPAAMRDRIGEMGAYQIQLAIQKQLQDTDMNKNHGRLSFPAKKLKVDFATEEERRVLKQQ 171

Query: 61  DEHQKNKSGVSALILDPCLRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQL 120
           +   K K G++ +I+DP LRES I +K WKIGS + YCL++QWN+L +QN  +SGD++QL
Sbjct: 172 E--NKGKKGMNVMIVDPLLRESSICLKLWKIGSGDSYCLMTQWNSLVEQNGFKSGDHVQL 231

Query: 121 WSFRKN-----NGTSPPQLCFALVKLD 143
           WSFRK+       T   +LCFA+VKLD
Sbjct: 232 WSFRKDELEAETQTMVSRLCFAMVKLD 256

[Close]

BLAST of MS016823 vs. TAIR 10
Match: AT1G05920.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 78.2 bits (191), Expect = 6.1e-15
Identity = 44/133 (33.08%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 13  GGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVS 72
           G  D +++I+K L   D++    RLSIPI  ++ NDF T +E +++D+ +   +   GV+
Sbjct: 176 GAGDPKLIIEKNLDSNDVDPRQNRLSIPINTVIQNDFLTLDESRLIDEDEITNEGNMGVA 235

Query: 73  ALILDPCLRESVIHVKKWKI-----GSREIYCLVSQWNALAQQNSLESGDYIQLWSFRKN 132
           A ++D   ++  +  K+W +      S   + L  +W+ + + N L+ GD I LWSFR N
Sbjct: 236 AFLVDQRTKKWNMGFKQWFMTTDSGSSYWSFVLRGEWSNVVETNGLKEGDKISLWSFRSN 295

Query: 133 NGTSPPQLCFALV 140
           +      LCFALV
Sbjct: 296 D-----ILCFALV 303

[Close]

BLAST of MS016823 vs. TAIR 10
Match: AT2G27410.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 75.5 bits (184), Expect = 3.9e-14
Identity = 45/131 (34.35%), Postives = 72/131 (54.96%), Query Frame = 0


Query: 11  ELGGYDIQIVIQKRLQDTDLNRNHGRLSIPIKKLLN-DFATEEEKKVLDQQDEHQKNKSG 70
           E  GY+ +++  ++L  TDL +   RLS+P K++   DF TE+E +++  ++  +   +G
Sbjct: 80  EENGYNPKLISTRQLYKTDLKKTEARLSVPFKQVKTPDFLTEDETRII-HENAMKIRDNG 139

Query: 71  VSALILDPCLRESVIHVKKWKIGSREIYCLVSQW-NAL-AQQNSLESGDYIQLWSFRKNN 130
           V    +DP L + V+ ++KWK+    IY  V  W N L A +   +  D   LWSFR   
Sbjct: 140 VPVNFVDPELNKHVLELRKWKMKGNWIYVFVKGWKNVLDACKTLFKEDDVYPLWSFRSGT 199

Query: 131 GTSPPQLCFAL 139
           G    +LCFAL
Sbjct: 200 G----KLCFAL 205

[Close]

BLAST of MS016823 vs. TAIR 10
Match: AT5G24050.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 72.4 bits (176), Expect = 3.3e-13
Identity = 40/115 (34.78%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 16  DIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVSALI 75
           D +++ +K L  TD+N    RLS+P   LL NDF T  E  +++ +D +  NK GV A++
Sbjct: 215 DAKLIFEKTLFVTDVNPTKNRLSMPFNNLLRNDFLTSVESIIIN-EDINNNNKIGVGAIL 274

Query: 76  LDPCLRESVIHVKKWKIGSRE-----IYCLVSQWNALAQQNSLESGDYIQLWSFR 125
           +D   ++  + +K+W++          Y L+  WN + + N L+ GD I LWSFR
Sbjct: 275 VDQRCKKWGVMLKRWEMKKESGKGSWSYNLICGWNDIVEANRLKEGDNITLWSFR 328

[Close]

BLAST of MS016823 vs. TAIR 10
Match: AT3G24850.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 71.6 bits (174), Expect = 5.7e-13
Identity = 42/129 (32.56%), Postives = 69/129 (53.49%), Query Frame = 0


Query: 16  DIQIVIQKRLQDTDLNRNHGRLSIPIKKLL-NDFATEEEKKVLDQQDEHQKNKSGVSALI 75
           D +++ +K L  TD+N    RLS+P   LL NDF T  E +++ ++D +   K GV A++
Sbjct: 225 DAKLIFEKTLFVTDINPTQNRLSMPFNNLLQNDFLTSVESRII-KEDINNNKKIGVGAIL 284

Query: 76  LDPCLRESVIHVKKWKIGSRE-----IYCLVSQWNALAQQNSLESGDYIQLWSFRKNNGT 135
           +D    +  + +K+W++          Y L+  WN + + N L+ GD I +WSFR     
Sbjct: 285 VDQRSVKWGVMLKRWELKKESGKGSWNYNLICGWNDVVEANGLKEGDNISVWSFRCRG-- 344

Query: 136 SPPQLCFAL 139
               LCFA+
Sbjct: 345 ---VLCFAM 347

[Close]

BLAST of MS016823 vs. TAIR 10
Match: AT2G24670.1 (Domain of unknown function (DUF313) )

HSP 1 Score: 68.6 bits (166), Expect = 4.8e-12
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 20  VIQKRLQDTDLNRNHGRLSIPIKKLLN-DFATEEEKKVLDQQDEHQKNKSGVSALILDPC 79
           +I K L  TD++ +  RLSIP+ +++  +F   EEK+ + ++D ++  K GV A+++D  
Sbjct: 132 IIDKELFQTDVDPHQSRLSIPVSQIVELEFLNHEEKRAI-EEDANRVRKEGVDAILVDSH 191

Query: 80  LRESVIHVKKWKIGSREIYCLVSQWNALAQQNSLESGDYIQLWSFRKNNGTSPPQLCFAL 139
           LRE  ++++   +    +Y LV+ WN +     LE    I+LWSF  ++      L FAL
Sbjct: 192 LREFPVNLRLRDMRGILLYNLVAGWNQVVTDCLLEENTNIRLWSFHADD-----TLYFAL 248

Query: 140 VKL 142
           V L
Sbjct: 252 VPL 248

The following BLAST results are available for this feature:

BLAST of MS016823 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144152.1	5.8e-68	88.89	B3 domain-containing protein At2g31420-like [Momordica charantia]	[more]
XP_022144153.1	2.1e-54	97.35	uncharacterized protein LOC111013910 [Momordica charantia]	[more]
XP_008445565.1	1.3e-43	62.34	PREDICTED: B3 domain-containing protein At3g25182-like [Cucumis melo] >KAA006523...	[more]
XP_004152777.2	5.0e-43	59.74	putative B3 domain-containing protein At2g27410 [Cucumis sativus] >KAE8652107.1 ...	[more]
KAG6598443.1	1.4e-42	59.86	B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS016823 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5RM09	8.6e-14	33.08	B3 domain-containing protein At1g05920 OS=Arabidopsis thaliana OX=3702 GN=At1g05...	[more]
Q9LSF7	1.9e-13	34.88	B3 domain-containing protein At3g25182 OS=Arabidopsis thaliana OX=3702 GN=At3g25...	[more]
Q9XIP5	5.6e-13	34.35	Putative B3 domain-containing protein At2g27410 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FLV7	4.7e-12	34.78	B3 domain-containing protein At5g24050 OS=Arabidopsis thaliana OX=3702 GN=At5g24...	[more]
Q9LRX6	8.0e-12	32.56	Putative B3 domain-containing protein At3g24850 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MS016823 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CSV9	2.8e-68	88.89	B3 domain-containing protein At2g31420-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1CSJ5	1.0e-54	97.35	uncharacterized protein LOC111013910 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A5A7VHB3	6.3e-44	62.34	B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A1S3BCJ5	6.3e-44	62.34	B3 domain-containing protein At3g25182-like OS=Cucumis melo OX=3656 GN=LOC103488...	[more]
A0A6J1H5Z0	2.7e-42	59.18	putative B3 domain-containing protein At3g24850 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MS016823 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05920.1	6.1e-15	33.08	Domain of unknown function (DUF313)	[more]
AT2G27410.1	3.9e-14	34.35	Domain of unknown function (DUF313)	[more]
AT5G24050.1	3.3e-13	34.78	Domain of unknown function (DUF313)	[more]
AT3G24850.1	5.7e-13	32.56	Domain of unknown function (DUF313)	[more]
AT2G24670.1	4.8e-12	33.33	Domain of unknown function (DUF313)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005508	B3 domain-containing protein At2g31720-like	PFAM	PF03754	DUF313	coord: 9..106 e-value: 5.6E-21 score: 74.9
IPR005508	B3 domain-containing protein At2g31720-like	PANTHER	PTHR31541	B3 DOMAIN PLANT PROTEIN-RELATED	coord: 2..141
IPR015300	DNA-binding pseudobarrel domain superfamily	GENE3D	2.40.330.10		coord: 14..136 e-value: 4.8E-14 score: 53.9
IPR015300	DNA-binding pseudobarrel domain superfamily	SUPERFAMILY	101936	DNA-binding pseudobarrel domain	coord: 20..126
None	No IPR available	PANTHER	PTHR31541:SF25	DOMAIN PROTEIN, PUTATIVE (DUF313)-RELATED	coord: 2..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS016823.1	MS016823.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003677	DNA binding